AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0842630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0842630 MtrunA17_Chr5g0413131 82.112 464 27 3 1 409 1 463 0.0 763
Msa0842630 MtrunA17_Chr8g0368161 58.929 448 109 8 18 409 20 448 1.98e-172 489
Msa0842630 MtrunA17_Chr4g0058771 54.945 455 137 6 23 409 27 481 2.12e-168 480
Msa0842630 MtrunA17_Chr3g0117271 60.526 380 93 5 86 409 118 496 2.80e-162 465
Msa0842630 MtrunA17_Chr2g0314461 57.143 385 111 4 77 409 24 406 2.91e-151 434
Msa0842630 MtrunA17_Chr3g0129891 55.754 391 140 10 23 408 17 379 3.09e-144 415
Msa0842630 MtrunA17_Chr2g0314471 51.716 408 140 6 57 409 1 406 4.28e-141 408
Msa0842630 MtrunA17_Chr2g0314451 51.232 406 143 5 58 409 1 405 1.28e-137 399
Msa0842630 MtrunA17_Chr8g0368291 47.229 415 157 8 47 409 1 405 3.56e-125 367
Msa0842630 MtrunA17_Chr1g0189961 45.803 417 138 7 81 409 35 451 2.84e-121 359
Msa0842630 MtrunA17_Chr8g0368341 51.351 333 119 5 119 409 4 335 1.91e-111 330
Msa0842630 MtrunA17_Chr8g0380511 47.895 380 124 9 102 408 37 415 1.34e-110 331
Msa0842630 MtrunA17_Chr2g0309251 46.354 384 128 10 102 409 45 426 5.36e-102 309
Msa0842630 MtrunA17_Chr7g0274951 70.619 194 56 1 216 408 246 439 1.48e-98 301
Msa0842630 MtrunA17_Chr7g0274951 32.663 199 121 3 62 248 15 212 9.37e-25 105
Msa0842630 MtrunA17_Chr8g0368181 70.466 193 55 2 218 409 2 193 6.60e-95 282
Msa0842630 MtrunA17_Chr8g0368241 62.264 212 73 2 199 409 209 414 1.83e-92 284
Msa0842630 MtrunA17_Chr8g0368241 53.804 184 77 5 47 228 1 178 3.80e-53 182
Msa0842630 MtrunA17_Chr2g0326371 47.634 317 103 7 148 409 52 360 4.09e-85 263
Msa0842630 MtrunA17_Chr8g0368201 63.212 193 68 3 219 409 129 320 1.33e-83 258
Msa0842630 MtrunA17_Chr8g0368201 60.345 58 22 1 163 220 5 61 2.94e-13 70.1
Msa0842630 MtrunA17_Chr8g0368221 48.097 289 69 4 47 285 1 258 2.50e-79 245
Msa0842630 MtrunA17_Chr8g0368361 54.639 194 86 1 218 409 2 195 2.44e-74 230
Msa0842630 MtrunA17_Chr8g0368281 53.368 193 88 2 218 408 2 194 1.70e-67 213
Msa0842630 MtrunA17_Chr8g0368331 51.031 194 92 3 219 409 93 286 6.65e-67 214
Msa0842630 MtrunA17_Chr8g0368351 54.301 186 66 5 20 204 17 184 2.34e-58 191
Msa0842630 MtrunA17_Chr8g0368271 58.228 158 60 3 47 203 1 153 5.17e-55 180
Msa0842630 MtrunA17_Chr8g0368231 66.400 125 41 1 286 409 8 132 5.32e-55 178
Msa0842630 MtrunA17_Chr1g0151871 35.862 290 137 6 167 409 40 327 2.53e-52 177
Msa0842630 MtrunA17_Chr6g0453411 40.254 236 126 5 177 409 152 375 1.64e-50 174
Msa0842630 MtrunA17_Chr6g0453411 44.565 92 44 3 118 203 8 98 1.12e-15 78.2
Msa0842630 MtrunA17_Chr8g0368261 61.417 127 46 3 285 409 4 129 4.05e-48 160
Msa0842630 MtrunA17_Chr8g0368321 53.020 149 59 3 61 207 7 146 3.56e-44 151
Msa0842630 MtrunA17_Chr8g0368381 56.637 113 49 0 218 330 2 114 5.62e-43 147
Msa0842630 MtrunA17_Chr3g0135401 38.362 232 129 3 177 405 126 346 2.09e-42 152
Msa0842630 MtrunA17_Chr3g0135381 43.077 195 100 4 216 404 243 432 5.39e-41 151
Msa0842630 MtrunA17_Chr3g0135381 46.296 108 51 2 102 203 52 158 1.38e-22 99.4
Msa0842630 MtrunA17_Chr3g0135371 35.865 237 137 4 177 409 210 435 7.41e-40 147
Msa0842630 MtrunA17_Chr3g0135371 39.216 153 80 4 70 215 25 171 1.25e-22 99.4
Msa0842630 MtrunA17_Chr8g0368171 52.381 147 61 5 47 192 1 139 1.48e-38 137
Msa0842630 MtrunA17_Chr3g0135441 37.433 187 105 4 225 404 45 226 1.35e-34 128
Msa0842630 MtrunA17_Chr3g0138811 28.934 394 198 16 67 405 20 386 2.32e-33 129
Msa0842630 MtrunA17_Chr8g0339871 38.889 198 108 8 216 403 43 237 3.43e-30 116
Msa0842630 MtrunA17_Chr8g0391201 37.245 196 112 7 216 403 43 235 3.23e-28 111
Msa0842630 MtrunA17_Chr2g0302691 28.736 348 172 13 116 403 37 368 9.36e-26 107
Msa0842630 MtrunA17_Chr3g0135431 36.735 196 103 6 62 249 14 196 1.75e-24 104
Msa0842630 MtrunA17_Chr8g0353941 37.433 187 108 6 223 405 1 182 2.15e-24 99.4
Msa0842630 MtrunA17_Chr3g0135451 35.802 162 74 5 62 203 8 159 1.08e-22 95.1
Msa0842630 MtrunA17_Chr8g0353971 37.647 170 99 5 238 403 4 170 2.14e-22 93.6
Msa0842630 MtrunA17_Chr2g0302651 35.795 176 102 7 237 403 23 196 2.15e-21 91.7
Msa0842630 MtrunA17_Chr5g0445181 28.163 245 115 8 149 344 65 297 2.41e-18 85.1
Msa0842630 MtrunA17_Chr5g0434771 37.931 145 84 5 244 386 24 164 4.04e-18 82.0
Msa0842630 MtrunA17_Chr8g0353961 39.423 104 58 3 102 201 33 135 4.31e-17 78.2
Msa0842630 MtrunA17_Chr8g0353931 36.449 107 57 3 102 201 33 135 8.06e-15 72.8
Msa0842630 MtrunA17_Chr5g0426971 33.077 130 79 3 235 362 90 213 3.00e-14 72.4
Msa0842630 MtrunA17_Chr8g0368191 46.667 75 37 3 60 133 5 77 2.05e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0842630 AT1G23340.2 62.438 402 95 4 64 409 8 409 0.0 510
Msa0842630 AT1G23340.1 62.438 402 95 4 64 409 8 409 0.0 510
Msa0842630 AT1G10750.1 65.172 379 75 4 87 409 90 467 4.08e-177 502
Msa0842630 AT1G70550.1 64.211 380 81 3 85 409 86 465 8.83e-177 501
Msa0842630 AT5G50150.1 62.069 377 87 3 89 409 44 420 8.40e-167 474
Msa0842630 AT5G18460.1 47.870 399 131 8 86 408 32 429 1.43e-120 357
Msa0842630 AT1G55360.1 44.076 422 152 9 61 409 12 422 2.23e-110 331
Msa0842630 AT3G13510.1 45.526 380 134 9 102 409 41 419 5.11e-106 319
Msa0842630 AT5G56530.2 44.855 379 136 8 102 408 42 419 1.73e-102 310
Msa0842630 AT5G56530.1 44.855 379 136 8 102 408 42 419 1.73e-102 310
Msa0842630 AT2G44220.1 40.831 409 173 9 64 409 1 403 2.14e-97 297
Msa0842630 AT2G44210.1 61.735 196 73 1 216 409 220 415 1.54e-86 269
Msa0842630 AT2G44210.1 38.519 135 64 4 102 218 34 167 8.13e-20 91.3
Msa0842630 AT2G44210.2 61.735 196 73 1 216 409 250 445 2.00e-86 270
Msa0842630 AT2G44210.2 32.121 165 63 5 102 218 34 197 4.30e-15 77.0
Msa0842630 AT2G44240.1 40.379 369 158 7 102 409 35 402 7.50e-85 265
Msa0842630 AT2G17750.1 38.108 370 162 11 102 408 30 395 1.87e-82 258
Msa0842630 AT3G48230.1 55.224 201 87 3 211 409 174 373 1.72e-71 229
Msa0842630 AT5G19170.1 37.027 370 167 8 94 403 2 365 2.71e-69 224
Msa0842630 AT1G23340.3 42.553 282 97 6 64 285 8 284 2.28e-61 201
Msa0842630 AT2G19360.1 50.754 199 97 1 211 408 227 425 2.27e-60 202
Msa0842630 AT2G19360.1 34.066 182 101 6 102 272 35 208 1.59e-21 96.3
Msa0842630 AT1G70550.2 68.657 134 41 1 85 218 86 218 6.98e-60 196
Msa0842630 AT2G44250.2 49.010 202 98 4 211 408 125 325 4.04e-57 190
Msa0842630 AT2G20170.1 32.754 403 194 13 64 403 8 396 7.99e-57 192
Msa0842630 AT2G20170.2 32.754 403 194 13 64 403 8 396 7.99e-57 192
Msa0842630 AT2G44250.1 49.010 202 98 4 211 408 208 408 9.91e-57 192
Msa0842630 AT2G44250.1 51.402 107 43 3 102 203 38 140 3.97e-19 89.0
Msa0842630 AT5G25960.1 37.243 341 173 8 102 409 25 357 2.88e-56 189
Msa0842630 AT2G20170.3 33.420 386 197 13 64 403 8 379 6.10e-56 189
Msa0842630 AT1G10750.2 63.636 132 47 1 87 218 90 220 3.99e-54 183
Msa0842630 AT4G23370.1 31.398 379 190 11 91 403 27 401 4.56e-51 177
Msa0842630 AT5G25950.1 42.424 198 109 3 216 409 236 432 7.27e-45 161
Msa0842630 AT5G25950.1 33.333 192 101 6 50 231 8 182 1.02e-14 75.9
Msa0842630 AT4G23360.1 30.189 371 184 11 100 403 37 399 4.00e-44 158
Msa0842630 AT4G23365.1 29.589 365 189 9 102 403 39 398 6.37e-44 158
Msa0842630 AT4G23355.1 30.208 384 194 11 86 403 23 398 5.55e-43 155
Msa0842630 AT2G35250.1 31.231 333 162 10 123 405 24 339 2.60e-36 136
Msa0842630 AT4G15050.1 29.412 374 175 14 102 403 38 394 7.37e-34 130
Msa0842630 AT4G23390.1 39.594 197 109 6 214 403 204 397 6.01e-33 128
Msa0842630 AT4G17505.1 28.955 335 168 12 114 409 18 321 1.16e-32 125
Msa0842630 AT2G27320.1 31.410 312 158 11 106 375 15 312 3.51e-30 119
Msa0842630 AT1G10190.1 28.218 404 203 16 70 403 8 394 4.30e-29 117
Msa0842630 AT4G23380.1 35.714 196 111 7 214 403 212 398 4.29e-28 114
Msa0842630 AT4G23380.1 33.884 121 73 3 92 208 32 149 2.52e-14 74.7
Msa0842630 AT4G23350.1 32.653 196 123 5 214 403 208 400 3.63e-27 112
Msa0842630 AT5G46810.1 27.566 341 182 11 126 403 11 349 1.10e-24 104
Msa0842630 AT5G46820.1 28.070 342 189 13 117 403 8 347 1.35e-24 104
Msa0842630 AT4G23373.1 31.818 198 93 4 100 259 34 227 2.18e-23 99.8
Msa0842630 AT4G10220.1 25.974 385 190 15 89 403 39 398 1.30e-21 96.3
Msa0842630 AT5G25410.1 24.425 348 197 11 110 403 28 363 1.57e-21 95.5
Msa0842630 AT4G17860.1 28.487 337 168 15 126 403 29 351 1.98e-20 92.4
Msa0842630 AT4G23080.1 26.582 395 185 19 89 405 39 406 2.08e-20 92.8
Msa0842630 AT5G05030.1 25.464 377 200 15 89 405 4 359 2.46e-20 92.0
Msa0842630 AT5G60380.1 25.449 334 183 13 126 403 44 367 3.45e-19 89.0
Msa0842630 AT5G46200.1 31.959 194 122 7 216 403 216 405 1.12e-18 87.8
Msa0842630 AT5G25415.1 31.373 204 116 7 216 405 139 332 2.09e-18 86.3
Msa0842630 AT4G10210.1 23.333 390 202 13 47 366 1 363 1.42e-16 81.3
Msa0842630 AT5G11660.1 30.102 196 125 5 216 405 115 304 1.26e-15 77.4
Msa0842630 AT4G15053.1 29.245 212 128 9 203 403 201 401 1.67e-15 78.2
Msa0842630 AT4G15053.1 34.615 130 78 3 70 196 12 137 2.51e-14 74.7
Msa0842630 AT2G38255.1 29.474 190 86 6 126 274 14 196 2.69e-15 75.1
Msa0842630 AT5G04937.1 30.256 195 110 7 216 403 23 198 2.27e-13 69.3
Msa0842630 AT5G37520.1 30.114 176 107 9 235 404 33 198 1.16e-12 67.0
Msa0842630 AT2G24950.1 33.696 92 61 0 102 193 56 147 2.06e-12 68.9
Msa0842630 AT5G36680.1 29.954 217 128 9 204 408 127 331 2.99e-12 67.8
Msa0842630 AT5G36770.1 29.954 217 128 9 204 408 127 331 2.99e-12 67.8

Find 120 sgRNAs with CRISPR-Local

Find 315 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGGTTTAAAAGTGTTAT+TGG 0.175466 5_4:+19771664 None:intergenic
ACCCTAGATTCTTTACTTAT+TGG 0.178065 5_4:-19769752 Msa0842630:CDS
GGAAATTGGTGGCTTGAATT+TGG 0.193828 5_4:-19767580 Msa0842630:CDS
TAATACCCTTGTCAAATCTA+AGG 0.197945 5_4:-19767348 Msa0842630:CDS
TTTGATCATCCTATGTTAAA+AGG 0.226362 5_4:-19771393 Msa0842630:CDS
TTGGAAAAGCATCATACTTT+AGG 0.235413 5_4:-19767402 Msa0842630:CDS
TGCTACAATTTATTGTGTTC+TGG 0.247960 5_4:-19769559 Msa0842630:CDS
CCTTGTATGTCATAGCAATT+TGG 0.252357 5_4:+19767310 None:intergenic
AGCCACCAATTTCCATGCTT+TGG 0.286320 5_4:+19767589 None:intergenic
TGTTCCGTTGTTCTTCTGAT+TGG 0.289858 5_4:+19771137 None:intergenic
TTGATATTAGCTTGCTCATT+TGG 0.296514 5_4:-19769462 Msa0842630:CDS
TCTCCAACTTCTTCATATAC+TGG 0.302096 5_4:-19769493 Msa0842630:CDS
AGGATCCAAAGCATGGAAAT+TGG 0.304845 5_4:-19767594 Msa0842630:CDS
CACTAATCTAGGCATTTATA+AGG 0.305785 5_4:-19772203 Msa0842630:five_prime_UTR
GAAGGGCATTCAAGGTGCTT+GGG 0.313719 5_4:-19767133 Msa0842630:three_prime_UTR
CAAAACCTTCTCCAGCAAAA+TGG 0.320633 5_4:+19767422 None:intergenic
TTAAAAGTGTTATTGGCAAC+AGG 0.326767 5_4:+19771671 None:intergenic
TGAATTTGGGTCCGGAATAT+TGG 0.327218 5_4:-19767566 Msa0842630:CDS
ACCTGAATTGTCTTAATAGA+AGG 0.330187 5_4:+19771587 None:intergenic
AAGGAGGAATAAATAATGTT+TGG 0.330732 5_4:-19767291 Msa0842630:CDS
AGGAGGAATAAATAATGTTT+GGG 0.337415 5_4:-19767290 Msa0842630:CDS
GGACATTTCACATTTCTTCC+TGG 0.340362 5_4:+19767244 None:intergenic
CCTTGCCACAATCAAAATCT+AGG 0.342060 5_4:-19771911 Msa0842630:CDS
AGTTTGAACAATTCTTCCTT+TGG 0.342698 5_4:+19771647 None:intergenic
CATGCAAGTATGGTACAATT+TGG 0.342749 5_4:-19767502 Msa0842630:CDS
CTGATTGGAATTGTTCCTTC+CGG 0.346847 5_4:+19771152 None:intergenic
CAGGTCAGCCCTGAGCTCTA+TGG 0.355105 5_4:-19769783 Msa0842630:CDS
GCTAGTTCTATCAATACATT+TGG 0.356680 5_4:-19771101 Msa0842630:CDS
GAAATTGGTGGCTTGAATTT+GGG 0.362311 5_4:-19767579 Msa0842630:CDS
ACCTTCTATTAAGACAATTC+AGG 0.364032 5_4:-19771588 Msa0842630:intron
CTTAAGGGATCATGCAAGTA+TGG 0.364508 5_4:-19767512 Msa0842630:CDS
ACCTTGAATGCCCTTCAAAA+AGG 0.366393 5_4:+19767140 None:intergenic
TGAGCAAGCTAATATCAAAT+TGG 0.367290 5_4:+19769467 None:intergenic
TGAAGGGCATTCAAGGTGCT+TGG 0.368886 5_4:-19767134 Msa0842630:three_prime_UTR
GTCCAATAAGTAAAGAATCT+AGG 0.379605 5_4:+19769750 None:intergenic
GGCCTAAAACTCATAACAAC+AGG 0.385123 5_4:-19771226 Msa0842630:CDS
CACGATTTCATCACTAATCT+AGG 0.386465 5_4:-19772214 Msa0842630:five_prime_UTR
CAACGGAACAAGACATGTTA+AGG 0.387654 5_4:-19771124 Msa0842630:CDS
TGTACAAGATTGTAAATGAT+TGG 0.389335 5_4:+19771755 None:intergenic
GAATTTGCAAGTTGTGGATT+GGG 0.390025 5_4:-19767380 Msa0842630:CDS
TCATGTACATTAAATAATCT+TGG 0.390780 5_4:-19772338 None:intergenic
GTCACACACTTCAACTCAAA+TGG 0.392629 5_4:-19767452 Msa0842630:CDS
TTTCAGTCATGGAGCTTGTC+GGG 0.407788 5_4:-19771185 Msa0842630:CDS
CCAATACCCTACCAATATTC+CGG 0.408965 5_4:+19767555 None:intergenic
AGGTCAGCCCTGAGCTCTAT+GGG 0.413270 5_4:-19769782 Msa0842630:CDS
GGAATTTGCAAGTTGTGGAT+TGG 0.413386 5_4:-19767381 Msa0842630:CDS
TTAAAATACATGAAAGGGTT+GGG 0.417328 5_4:+19772147 None:intergenic
AACTTGAGCTCTCAGGAGTT+GGG 0.421788 5_4:+19772117 None:intergenic
CTCCAACTTCTTCATATACT+GGG 0.426503 5_4:-19769492 Msa0842630:CDS
GAGTCAAAACTTGTAAAACT+TGG 0.437303 5_4:+19772080 None:intergenic
CAACTTGAGCTCTCAGGAGT+TGG 0.447058 5_4:+19772116 None:intergenic
ACTAACAATAAAATTGCAAT+TGG 0.449628 5_4:-19769526 Msa0842630:CDS
AGTGATGCATATCAAGCAAC+TGG 0.451463 5_4:-19769583 Msa0842630:CDS
TGGTGGCTTGAATTTGGGTC+CGG 0.451727 5_4:-19767574 Msa0842630:CDS
ATAGTATAGTATAGCTTTGG+TGG 0.453727 5_4:-19771946 Msa0842630:five_prime_UTR
CTTTAGGAATTTGCAAGTTG+TGG 0.454834 5_4:-19767386 Msa0842630:CDS
TCTATAGTATAGTATAGCTT+TGG 0.458492 5_4:-19771949 Msa0842630:five_prime_UTR
TGCAGTAATGGTATAATCAA+TGG 0.458539 5_4:+19767035 None:intergenic
ATTAAAATACATGAAAGGGT+TGG 0.461693 5_4:+19772146 None:intergenic
AAATATATGTTGAATTTCAA+AGG 0.465479 5_4:+19772300 None:intergenic
CCGGAATATTGGTAGGGTAT+TGG 0.467009 5_4:-19767555 Msa0842630:CDS
TAACTGTCCCCATAGAGCTC+AGG 0.469376 5_4:+19769774 None:intergenic
AAGAACAAATCCTAATCAAA+AGG 0.479596 5_4:+19767080 None:intergenic
TCCAATAAGTAAAGAATCTA+GGG 0.480244 5_4:+19769751 None:intergenic
AACATAGGATGATCAAAAGC+TGG 0.480414 5_4:+19771399 None:intergenic
GATGATTAAAATACATGAAA+GGG 0.482433 5_4:+19772142 None:intergenic
GAAAGGAAAATTCTCAAGCA+TGG 0.493123 5_4:-19771801 Msa0842630:CDS
ACTAATCTAGGCATTTATAA+GGG 0.499819 5_4:-19772202 Msa0842630:five_prime_UTR
AATACCCTTGTCAAATCTAA+GGG 0.502530 5_4:-19767347 Msa0842630:CDS
TCAATGTGAACTACTTCAAA+CGG 0.503736 5_4:-19771843 Msa0842630:CDS
TATTAGCTTGCTCATTTGGA+AGG 0.505252 5_4:-19769458 Msa0842630:intron
GGATGATTAAAATACATGAA+AGG 0.505297 5_4:+19772141 None:intergenic
AATTTATAGGAGTGTGTACA+AGG 0.510075 5_4:+19772248 None:intergenic
AGTCAAAACTTGTAAAACTT+GGG 0.516771 5_4:+19772081 None:intergenic
TAGGAGTGTGTACAAGGGAA+GGG 0.517712 5_4:+19772254 None:intergenic
TACTGACAACTTGAGCTCTC+AGG 0.522962 5_4:+19772110 None:intergenic
AACGGAACAAGACATGTTAA+GGG 0.533255 5_4:-19771123 Msa0842630:CDS
CATAAGAGTCCATGCAGTAA+TGG 0.534353 5_4:+19767023 None:intergenic
TCACACACTTCAACTCAAAT+GGG 0.534918 5_4:-19767451 Msa0842630:CDS
AGAAATGTGAAATGTCCCTA+AGG 0.537968 5_4:-19767238 Msa0842630:exon
AAGGGCATTCAAGGTGCTTG+GGG 0.539760 5_4:-19767132 Msa0842630:three_prime_UTR
GCAAGTATGGTACAATTTGG+TGG 0.547559 5_4:-19767499 Msa0842630:CDS
AATTATATACTATGCTTCCA+AGG 0.549037 5_4:-19772031 Msa0842630:five_prime_UTR
TTTGGGTCCGGAATATTGGT+AGG 0.551957 5_4:-19767562 Msa0842630:CDS
CCAAATTGCTATGACATACA+AGG 0.552229 5_4:-19767310 Msa0842630:CDS
TAGATTCTTTACTTATTGGA+CGG 0.552867 5_4:-19769748 Msa0842630:intron
AACTGTCCCCATAGAGCTCA+GGG 0.555050 5_4:+19769775 None:intergenic
ATAGGAGTGTGTACAAGGGA+AGG 0.556665 5_4:+19772253 None:intergenic
CTTGTCGGGTGAATCATGTC+CGG 0.562105 5_4:-19771171 Msa0842630:CDS
CATTCTATGATATCACCGTC+AGG 0.565456 5_4:+19771441 None:intergenic
TCCAACTTCTTCATATACTG+GGG 0.571043 5_4:-19769491 Msa0842630:CDS
TATAAGGGCTCCAACCCACA+TGG 0.577082 5_4:-19772187 Msa0842630:five_prime_UTR
ATCCAAAGCATGGAAATTGG+TGG 0.585312 5_4:-19767591 Msa0842630:CDS
TCTTCAATAGAAACAAAGAA+AGG 0.586169 5_4:-19771818 Msa0842630:CDS
GGAGGAATAAATAATGTTTG+GGG 0.587413 5_4:-19767289 Msa0842630:CDS
CACAAATAGCAATGGACATG+AGG 0.590650 5_4:-19771048 Msa0842630:intron
GTGTGCAGGATCCTCCAGAA+AGG 0.596318 5_4:-19771247 Msa0842630:intron
AGGAGTGTGTACAAGGGAAG+GGG 0.599342 5_4:+19772255 None:intergenic
TTGATTATACCATTACTGCA+TGG 0.602877 5_4:-19767032 Msa0842630:three_prime_UTR
TTGGGTCCGGAATATTGGTA+GGG 0.608291 5_4:-19767561 Msa0842630:CDS
GAAATGTGAAATGTCCCTAA+GGG 0.609542 5_4:-19767237 Msa0842630:exon
TAAAAGTGTTATTGGCAACA+GGG 0.614146 5_4:+19771672 None:intergenic
TTGAGCTCTCAGGAGTTGGG+TGG 0.617510 5_4:+19772120 None:intergenic
TAGAACCCTTAGATTTGACA+AGG 0.625722 5_4:+19767342 None:intergenic
ACTTCAACTCAAATGGGTAG+TGG 0.626407 5_4:-19767445 Msa0842630:CDS
AGAAGAGACACAAATAGCAA+TGG 0.629086 5_4:-19771056 Msa0842630:CDS
GTTAAAAGGACAAAGACCAC+TGG 0.629673 5_4:-19771379 Msa0842630:intron
TCGGGTGAATCATGTCCGGA+AGG 0.643411 5_4:-19771167 Msa0842630:CDS
GTCAAATCTAAGGGTTCTAG+CGG 0.646768 5_4:-19767338 Msa0842630:CDS
ATTTATAGGAGTGTGTACAA+GGG 0.650243 5_4:+19772249 None:intergenic
GAAATTGTGAATTCAAGACA+AGG 0.660538 5_4:-19767475 Msa0842630:CDS
ATAAGGGCTCCAACCCACAT+GGG 0.662992 5_4:-19772186 Msa0842630:five_prime_UTR
GAGGAATAAATAATGTTTGG+GGG 0.669410 5_4:-19767288 Msa0842630:CDS
AATTCCAATCAGAAGAACAA+CGG 0.674959 5_4:-19771141 Msa0842630:CDS
CCAACTTCTTCATATACTGG+GGG 0.674996 5_4:-19769490 Msa0842630:CDS
GCCTAAAACTCATAACAACA+GGG 0.692904 5_4:-19771225 Msa0842630:CDS
GGTCAGCCCTGAGCTCTATG+GGG 0.696304 5_4:-19769781 Msa0842630:CDS
AGAACCCTTAGATTTGACAA+GGG 0.704158 5_4:+19767343 None:intergenic
AAATTGTGAATTCAAGACAA+GGG 0.714404 5_4:-19767474 Msa0842630:CDS
AATTGCTATGACATACAAGG+AGG 0.724912 5_4:-19767307 Msa0842630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATAAAAAATAAAAT+TGG + chr5_4:19769945-19769964 None:intergenic 0.0%
!! TTAATAAAAAATAATATTAA+AGG + chr5_4:19770549-19770568 None:intergenic 0.0%
!! TTTATTATATGTATAAGTTA+AGG - chr5_4:19767987-19768006 Msa0842630:intron 10.0%
!! ATAATATTAAAGGTTAAAAA+TGG + chr5_4:19770539-19770558 None:intergenic 10.0%
!!! ATGATAATATATTTTTTTGA+AGG + chr5_4:19770761-19770780 None:intergenic 10.0%
!! AATTCTTAGTTAAAATATTT+AGG - chr5_4:19770872-19770891 Msa0842630:intron 10.0%
!! ATTCTTAGTTAAAATATTTA+GGG - chr5_4:19770873-19770892 Msa0842630:intron 10.0%
!!! TTTGTTATACTATTTTTTTT+AGG - chr5_4:19771694-19771713 Msa0842630:CDS 10.0%
!!! TTTTTTTTTCTTTTTCTTTT+TGG - chr5_4:19772116-19772135 Msa0842630:five_prime_UTR 10.0%
!! AAATATATGTTGAATTTCAA+AGG + chr5_4:19767011-19767030 None:intergenic 15.0%
!! TGCTAAGTTTAAAAATTTAT+AGG + chr5_4:19767076-19767095 None:intergenic 15.0%
!!! TATTTCAAGTTTGATTTTTT+TGG + chr5_4:19768694-19768713 None:intergenic 15.0%
!!! ATCATTTTGAATATAATTCA+AGG + chr5_4:19768780-19768799 None:intergenic 15.0%
!!! AGTATTTTGAATATAATCTA+AGG + chr5_4:19768923-19768942 None:intergenic 15.0%
!! TATACATCAATAAAAATTTG+AGG + chr5_4:19769046-19769065 None:intergenic 15.0%
!! AGATACAAAAATTAAAAACA+TGG + chr5_4:19769472-19769491 None:intergenic 15.0%
!! TATAAAACTCAATAAAGAAA+AGG - chr5_4:19769587-19769606 Msa0842630:intron 15.0%
!! ATAAAACTCAATAAAGAAAA+GGG - chr5_4:19769588-19769607 Msa0842630:intron 15.0%
!!! AAATTGTCAATAATTTTCTA+AGG + chr5_4:19769909-19769928 None:intergenic 15.0%
!!! AATTTAGTTTTACTATTGAT+TGG - chr5_4:19769974-19769993 Msa0842630:intron 15.0%
!!! ATAATTTATAAAACGGATTA+AGG - chr5_4:19770025-19770044 Msa0842630:intron 15.0%
!!! TAATTTATAAAACGGATTAA+GGG - chr5_4:19770026-19770045 Msa0842630:intron 15.0%
!!! TAGATTTGATTTTGTTTATA+CGG - chr5_4:19770698-19770717 Msa0842630:intron 15.0%
!!! TTATTTCTTTCGATTTATAT+TGG + chr5_4:19770808-19770827 None:intergenic 15.0%
!! TTCTTAGTTAAAATATTTAG+GGG - chr5_4:19770874-19770893 Msa0842630:intron 15.0%
!! TTTATTCACTTTAATACTTT+GGG - chr5_4:19771326-19771345 Msa0842630:intron 15.0%
!!! AAATTAAATTCCTTTTGATT+AGG - chr5_4:19772218-19772237 Msa0842630:five_prime_UTR 15.0%
!! GATGATTAAAATACATGAAA+GGG + chr5_4:19767169-19767188 None:intergenic 20.0%
!! AAAAATGAGTATTCTAAGTA+TGG + chr5_4:19767331-19767350 None:intergenic 20.0%
!!! AACAATTTTGTTTTTGTAGA+TGG - chr5_4:19767427-19767446 Msa0842630:CDS 20.0%
!! AAAAAAACAGAGTAAAAACA+GGG + chr5_4:19767781-19767800 None:intergenic 20.0%
!! TATGTATAAGTTAAGGTATA+AGG - chr5_4:19767994-19768013 Msa0842630:intron 20.0%
!! ATGTATAAGTTAAGGTATAA+GGG - chr5_4:19767995-19768014 Msa0842630:intron 20.0%
!! ATGAAAATGAGTATTCATAT+TGG - chr5_4:19768486-19768505 Msa0842630:intron 20.0%
!!! AACAATAAATTTTGAGTAGT+TGG - chr5_4:19768516-19768535 Msa0842630:intron 20.0%
!! ATCACTATAAATAGGAAAAA+AGG + chr5_4:19768567-19768586 None:intergenic 20.0%
!! AAAAAAATTTAATCTCACCA+AGG - chr5_4:19768752-19768771 Msa0842630:intron 20.0%
!! GTACAACAATAAAAACTATT+AGG + chr5_4:19769111-19769130 None:intergenic 20.0%
!!! AATCAATTAAGTTGAAGTAA+TGG + chr5_4:19769317-19769336 None:intergenic 20.0%
!!! AAACATGGAGAAAAATTTTA+TGG + chr5_4:19769457-19769476 None:intergenic 20.0%
!! ACTAACAATAAAATTGCAAT+TGG - chr5_4:19769782-19769801 Msa0842630:CDS 20.0%
!! AACAGATTTCTAAACTATAA+AGG + chr5_4:19770077-19770096 None:intergenic 20.0%
!!! TTAATTTGATCCATCAATAT+TGG + chr5_4:19770133-19770152 None:intergenic 20.0%
!!! AATATTGATGGATCAAATTA+AGG - chr5_4:19770132-19770151 Msa0842630:intron 20.0%
!!! ATATTGATGGATCAAATTAA+GGG - chr5_4:19770133-19770152 Msa0842630:intron 20.0%
!! AGTAAAAGATTCATATCAAA+GGG + chr5_4:19770190-19770209 None:intergenic 20.0%
!! TAGTAAAAGATTCATATCAA+AGG + chr5_4:19770191-19770210 None:intergenic 20.0%
!!! TTTGATATGAATCTTTTACT+AGG - chr5_4:19770190-19770209 Msa0842630:intron 20.0%
!! AAATGATTAAAAATGCTCAA+TGG - chr5_4:19770282-19770301 Msa0842630:intron 20.0%
!!! TTTTTTTTTTTTGTTCCTGT+TGG - chr5_4:19770659-19770678 Msa0842630:intron 20.0%
!!! AAGAATATTTTTTAACCAAC+AGG + chr5_4:19770677-19770696 None:intergenic 20.0%
!!! CTGATTTAAATTAAAGTGTT+TGG + chr5_4:19771005-19771024 None:intergenic 20.0%
!! CTATACTTCTAATATTCTAT+GGG + chr5_4:19771143-19771162 None:intergenic 20.0%
!!! CATAGAATATTAGAAGTATA+GGG - chr5_4:19771142-19771161 Msa0842630:CDS 20.0%
!!! ATAGAATATTAGAAGTATAG+GGG - chr5_4:19771143-19771162 Msa0842630:CDS 20.0%
!! AAGTATTAAAGTGAATAAAG+TGG + chr5_4:19771325-19771344 None:intergenic 20.0%
!! CTTTATTCACTTTAATACTT+TGG - chr5_4:19771325-19771344 Msa0842630:intron 20.0%
!! CAAAACACAAAAATAACAAA+AGG - chr5_4:19771656-19771675 Msa0842630:CDS 20.0%
!!! TTTTTCTTTTTGGCATTTTT+AGG - chr5_4:19772126-19772145 Msa0842630:five_prime_UTR 20.0%
! ACTAATCTAGGCATTTATAA+GGG - chr5_4:19767106-19767125 Msa0842630:three_prime_UTR 25.0%
! TTAAAATACATGAAAGGGTT+GGG + chr5_4:19767164-19767183 None:intergenic 25.0%
! ATTAAAATACATGAAAGGGT+TGG + chr5_4:19767165-19767184 None:intergenic 25.0%
! GGATGATTAAAATACATGAA+AGG + chr5_4:19767170-19767189 None:intergenic 25.0%
! AGTCAAAACTTGTAAAACTT+GGG + chr5_4:19767230-19767249 None:intergenic 25.0%
! AATTATATACTATGCTTCCA+AGG - chr5_4:19767277-19767296 Msa0842630:CDS 25.0%
! TCTATAGTATAGTATAGCTT+TGG - chr5_4:19767359-19767378 Msa0842630:CDS 25.0%
! TCTTCAATAGAAACAAAGAA+AGG - chr5_4:19767490-19767509 Msa0842630:CDS 25.0%
! TGTACAAGATTGTAAATGAT+TGG + chr5_4:19767556-19767575 None:intergenic 25.0%
!!! CTTTGGTTTAAAAGTGTTAT+TGG + chr5_4:19767647-19767666 None:intergenic 25.0%
!! CAATAACACTTTTAAACCAA+AGG - chr5_4:19767645-19767664 Msa0842630:intron 25.0%
! AAAAAACAGAGTAAAAACAG+GGG + chr5_4:19767780-19767799 None:intergenic 25.0%
! CAAAAAAACAGAGTAAAAAC+AGG + chr5_4:19767782-19767801 None:intergenic 25.0%
! TTTGATCATCCTATGTTAAA+AGG - chr5_4:19767915-19767934 Msa0842630:intron 25.0%
! AAACAAGAACTTCAATTTCT+TGG + chr5_4:19768322-19768341 None:intergenic 25.0%
! ATTGAAACATGAAAAACAGA+CGG + chr5_4:19768446-19768465 None:intergenic 25.0%
! ATTCTGCAATCACTATAAAT+AGG + chr5_4:19768575-19768594 None:intergenic 25.0%
!! ATATAATCTAAGGCTACTTA+AGG + chr5_4:19768913-19768932 None:intergenic 25.0%
! TAGAATATAAACACATGTGT+CGG + chr5_4:19769011-19769030 None:intergenic 25.0%
! ACTCTTAAATGCACTATATT+TGG - chr5_4:19769393-19769412 Msa0842630:intron 25.0%
!! ATGGAGAAAAATTTTATGGT+AGG + chr5_4:19769453-19769472 None:intergenic 25.0%
! TCCAATAAGTAAAGAATCTA+GGG + chr5_4:19769560-19769579 None:intergenic 25.0%
! TAGATTCTTTACTTATTGGA+CGG - chr5_4:19769560-19769579 Msa0842630:CDS 25.0%
! ACAAAAGTCTTCAAATTTCA+TGG - chr5_4:19770216-19770235 Msa0842630:intron 25.0%
! ATTAAAAATGCTCAATGGTA+GGG - chr5_4:19770287-19770306 Msa0842630:intron 25.0%
! CCATATATCACAAAATTCAA+TGG + chr5_4:19770340-19770359 None:intergenic 25.0%
!! CCATTGAATTTTGTGATATA+TGG - chr5_4:19770337-19770356 Msa0842630:intron 25.0%
! GTAGTGCTTAATATTTCTTA+CGG - chr5_4:19770604-19770623 Msa0842630:intron 25.0%
! AATCAAATAGAAACAACGTA+AGG + chr5_4:19770641-19770660 None:intergenic 25.0%
! CCTATACTTCTAATATTCTA+TGG + chr5_4:19771144-19771163 None:intergenic 25.0%
!! CCATAGAATATTAGAAGTAT+AGG - chr5_4:19771141-19771160 Msa0842630:CDS 25.0%
!! GAAGAGAATCTTTAAAAGAA+AGG + chr5_4:19771410-19771429 None:intergenic 25.0%
! TGATGAACAAAACTACAAAA+GGG + chr5_4:19771472-19771491 None:intergenic 25.0%
! ATGATGAACAAAACTACAAA+AGG + chr5_4:19771473-19771492 None:intergenic 25.0%
!! AAGGGTATGTACAAAATAAT+GGG + chr5_4:19771521-19771540 None:intergenic 25.0%
!!! GTTTTTGTTTACACACTTAA+GGG - chr5_4:19771781-19771800 Msa0842630:CDS 25.0%
! AAATTGTGAATTCAAGACAA+GGG - chr5_4:19771834-19771853 Msa0842630:CDS 25.0%
! AAGGAGGAATAAATAATGTT+TGG - chr5_4:19772017-19772036 Msa0842630:five_prime_UTR 25.0%
! AGGAGGAATAAATAATGTTT+GGG - chr5_4:19772018-19772037 Msa0842630:five_prime_UTR 25.0%
!!! TTTTTGGCATTTTTAGGTTT+GGG - chr5_4:19772132-19772151 Msa0842630:five_prime_UTR 25.0%
! AAGAACAAATCCTAATCAAA+AGG + chr5_4:19772231-19772250 None:intergenic 25.0%
! ATTTATAGGAGTGTGTACAA+GGG + chr5_4:19767062-19767081 None:intergenic 30.0%
! AATTTATAGGAGTGTGTACA+AGG + chr5_4:19767063-19767082 None:intergenic 30.0%
CACTAATCTAGGCATTTATA+AGG - chr5_4:19767105-19767124 Msa0842630:three_prime_UTR 30.0%
GAGTCAAAACTTGTAAAACT+TGG + chr5_4:19767231-19767250 None:intergenic 30.0%
! TTTCACTATTTTCAATGCCT+TGG + chr5_4:19767297-19767316 None:intergenic 30.0%
!! ATAGTATAGTATAGCTTTGG+TGG - chr5_4:19767362-19767381 Msa0842630:CDS 30.0%
TCAATGTGAACTACTTCAAA+CGG - chr5_4:19767465-19767484 Msa0842630:CDS 30.0%
!! ATTTTTGAGGAGGAAAAAAC+AGG + chr5_4:19767607-19767626 None:intergenic 30.0%
!! TAAAAGTGTTATTGGCAACA+GGG + chr5_4:19767639-19767658 None:intergenic 30.0%
!! TTAAAAGTGTTATTGGCAAC+AGG + chr5_4:19767640-19767659 None:intergenic 30.0%
AGTTTGAACAATTCTTCCTT+TGG + chr5_4:19767664-19767683 None:intergenic 30.0%
ACCTTCTATTAAGACAATTC+AGG - chr5_4:19767720-19767739 Msa0842630:intron 30.0%
ACCTGAATTGTCTTAATAGA+AGG + chr5_4:19767724-19767743 None:intergenic 30.0%
!!! AATTTTTTTCAGAGTCCTGA+CGG - chr5_4:19767852-19767871 Msa0842630:intron 30.0%
ACTCAATTAGAATTTACCAG+TGG + chr5_4:19767948-19767967 None:intergenic 30.0%
AATTCCAATCAGAAGAACAA+CGG - chr5_4:19768167-19768186 Msa0842630:intron 30.0%
!! GCTAGTTCTATCAATACATT+TGG - chr5_4:19768207-19768226 Msa0842630:intron 30.0%
TTCATTCCAAACAAGACAAA+TGG + chr5_4:19768471-19768490 None:intergenic 30.0%
ATAGTGATTGCAGAATTTCA+TGG - chr5_4:19768579-19768598 Msa0842630:intron 30.0%
!!! CCTAAATTTTTTCCTTGCAA+AGG + chr5_4:19768731-19768750 None:intergenic 30.0%
CCTTTGCAAGGAAAAAATTT+AGG - chr5_4:19768728-19768747 Msa0842630:intron 30.0%
GAATATAATTCAAGGTTCCT+TGG + chr5_4:19768772-19768791 None:intergenic 30.0%
TGTCATACGTGCATATATAT+CGG + chr5_4:19768834-19768853 None:intergenic 30.0%
AAATCAATACTATGGAGCAA+AGG - chr5_4:19769156-19769175 Msa0842630:intron 30.0%
ACCCTAGATTCTTTACTTAT+TGG - chr5_4:19769556-19769575 Msa0842630:CDS 30.0%
GTCCAATAAGTAAAGAATCT+AGG + chr5_4:19769561-19769580 None:intergenic 30.0%
TGCTACAATTTATTGTGTTC+TGG - chr5_4:19769749-19769768 Msa0842630:CDS 30.0%
TGAGCAAGCTAATATCAAAT+TGG + chr5_4:19769844-19769863 None:intergenic 30.0%
!! TTGATATTAGCTTGCTCATT+TGG - chr5_4:19769846-19769865 Msa0842630:intron 30.0%
AATCCAACAACCAATATTGA+TGG - chr5_4:19770120-19770139 Msa0842630:intron 30.0%
GATTAAAAATGCTCAATGGT+AGG - chr5_4:19770286-19770305 Msa0842630:intron 30.0%
! GTTTGTTACTTCCAAAACAA+CGG + chr5_4:19770316-19770335 None:intergenic 30.0%
!!! GTAGTTATTTCTTTTACGTG+GGG + chr5_4:19770580-19770599 None:intergenic 30.0%
!!! GGTAGTTATTTCTTTTACGT+GGG + chr5_4:19770581-19770600 None:intergenic 30.0%
AGAAATATTAAGCACTACGT+GGG + chr5_4:19770602-19770621 None:intergenic 30.0%
AAGAAATATTAAGCACTACG+TGG + chr5_4:19770603-19770622 None:intergenic 30.0%
TAATATTCTATGGGTGTGTT+TGG + chr5_4:19771134-19771153 None:intergenic 30.0%
GTTCATTATTCGAATTCACT+AGG + chr5_4:19771366-19771385 None:intergenic 30.0%
AGAATTAGAAGCTCTTTGTT+GGG + chr5_4:19771439-19771458 None:intergenic 30.0%
! GAAGGGTATGTACAAAATAA+TGG + chr5_4:19771522-19771541 None:intergenic 30.0%
AAAAAGAGGAGTAAGAAAAG+TGG + chr5_4:19771554-19771573 None:intergenic 30.0%
AAGTGAAATGAGTCAAAAAG+AGG + chr5_4:19771568-19771587 None:intergenic 30.0%
!!! TTTTTTTAGGATCCAAAGCA+TGG - chr5_4:19771707-19771726 Msa0842630:CDS 30.0%
AAGTGTGTAAACAAAAACGA+TGG + chr5_4:19771779-19771798 None:intergenic 30.0%
!! CGTTTTTGTTTACACACTTA+AGG - chr5_4:19771780-19771799 Msa0842630:CDS 30.0%
GAAATTGTGAATTCAAGACA+AGG - chr5_4:19771833-19771852 Msa0842630:CDS 30.0%
!! TTGGAAAAGCATCATACTTT+AGG - chr5_4:19771906-19771925 Msa0842630:CDS 30.0%
TAATACCCTTGTCAAATCTA+AGG - chr5_4:19771960-19771979 Msa0842630:five_prime_UTR 30.0%
AATACCCTTGTCAAATCTAA+GGG - chr5_4:19771961-19771980 Msa0842630:five_prime_UTR 30.0%
GGAGGAATAAATAATGTTTG+GGG - chr5_4:19772019-19772038 Msa0842630:five_prime_UTR 30.0%
GAGGAATAAATAATGTTTGG+GGG - chr5_4:19772020-19772039 Msa0842630:five_prime_UTR 30.0%
! TTTTATGCTTCATTCCCTTA+GGG + chr5_4:19772088-19772107 None:intergenic 30.0%
! TTTTTATGCTTCATTCCCTT+AGG + chr5_4:19772089-19772108 None:intergenic 30.0%
!!! CTTTTTGGCATTTTTAGGTT+TGG - chr5_4:19772131-19772150 Msa0842630:five_prime_UTR 30.0%
!!! TTTTTAGGTTTGGGTGATAT+TGG - chr5_4:19772141-19772160 Msa0842630:five_prime_UTR 30.0%
!!! ATATTGGTTCTCCTTTTTGA+AGG - chr5_4:19772157-19772176 Msa0842630:five_prime_UTR 30.0%
!!! TATTGGTTCTCCTTTTTGAA+GGG - chr5_4:19772158-19772177 Msa0842630:five_prime_UTR 30.0%
TGCAGTAATGGTATAATCAA+TGG + chr5_4:19772276-19772295 None:intergenic 30.0%
TTGATTATACCATTACTGCA+TGG - chr5_4:19772276-19772295 Msa0842630:five_prime_UTR 30.0%
CACGATTTCATCACTAATCT+AGG - chr5_4:19767094-19767113 Msa0842630:three_prime_UTR 35.0%
!! TATTCAAGTTTTGCCCATGT+GGG + chr5_4:19767138-19767157 None:intergenic 35.0%
!!! CTAGATTTTGATTGTGGCAA+GGG + chr5_4:19767399-19767418 None:intergenic 35.0%
!!! GTGTTCCTAGATTTTGATTG+TGG + chr5_4:19767405-19767424 None:intergenic 35.0%
GAAAGGAAAATTCTCAAGCA+TGG - chr5_4:19767507-19767526 Msa0842630:CDS 35.0%
!! GTTGTTGTCAATTTTTGAGG+AGG + chr5_4:19767617-19767636 None:intergenic 35.0%
! AGGGTTGTTGTCAATTTTTG+AGG + chr5_4:19767620-19767639 None:intergenic 35.0%
AACATAGGATGATCAAAAGC+TGG + chr5_4:19767912-19767931 None:intergenic 35.0%
! GGTCTTTGTCCTTTTAACAT+AGG + chr5_4:19767927-19767946 None:intergenic 35.0%
! TTATTTTCTTGCATGTGTGC+AGG - chr5_4:19768047-19768066 Msa0842630:intron 35.0%
!!! TTATGAGTTTTAGGCCTTTC+TGG + chr5_4:19768078-19768097 None:intergenic 35.0%
GCCTAAAACTCATAACAACA+GGG - chr5_4:19768083-19768102 Msa0842630:intron 35.0%
! TCCCTGTTGTTATGAGTTTT+AGG + chr5_4:19768087-19768106 None:intergenic 35.0%
AACGGAACAAGACATGTTAA+GGG - chr5_4:19768185-19768204 Msa0842630:intron 35.0%
AGAAGAGACACAAATAGCAA+TGG - chr5_4:19768252-19768271 Msa0842630:intron 35.0%
! CAATGTCCATTTGTCTTGTT+TGG - chr5_4:19768462-19768481 Msa0842630:intron 35.0%
ACTATCACATTACCTTGTGT+AGG - chr5_4:19768658-19768677 Msa0842630:intron 35.0%
CTATCACATTACCTTGTGTA+GGG - chr5_4:19768659-19768678 Msa0842630:intron 35.0%
! TCTGAAGTGTCACTATTGTA+TGG + chr5_4:19768946-19768965 None:intergenic 35.0%
TGTAGACACCTGACATATAT+CGG + chr5_4:19768988-19769007 None:intergenic 35.0%
GTGACTGGAAATCAATACTA+TGG - chr5_4:19769148-19769167 Msa0842630:intron 35.0%
!! ATATTGATTTGCAACACGTG+GGG + chr5_4:19769200-19769219 None:intergenic 35.0%
!! CATATTGATTTGCAACACGT+GGG + chr5_4:19769201-19769220 None:intergenic 35.0%
!! TCATATTGATTTGCAACACG+TGG + chr5_4:19769202-19769221 None:intergenic 35.0%
AAACCTTTGCTTGAAGTGTA+AGG - chr5_4:19769647-19769666 Msa0842630:intron 35.0%
TCTCCAACTTCTTCATATAC+TGG - chr5_4:19769815-19769834 Msa0842630:intron 35.0%
CTCCAACTTCTTCATATACT+GGG - chr5_4:19769816-19769835 Msa0842630:intron 35.0%
TCCAACTTCTTCATATACTG+GGG - chr5_4:19769817-19769836 Msa0842630:intron 35.0%
!! TATTAGCTTGCTCATTTGGA+AGG - chr5_4:19769850-19769869 Msa0842630:intron 35.0%
!! TGTTTTGAGCATATACACGT+TGG - chr5_4:19770093-19770112 Msa0842630:intron 35.0%
! GATCCATCAATATTGGTTGT+TGG + chr5_4:19770126-19770145 None:intergenic 35.0%
CAATCTTCTATCCTTTAGGT+GGG + chr5_4:19770160-19770179 None:intergenic 35.0%
TCAATCTTCTATCCTTTAGG+TGG + chr5_4:19770161-19770180 None:intergenic 35.0%
GGATCAATCTTCTATCCTTT+AGG + chr5_4:19770164-19770183 None:intergenic 35.0%
AAGATTCATATCAAAGGGCA+GGG + chr5_4:19770185-19770204 None:intergenic 35.0%
AAAGATTCATATCAAAGGGC+AGG + chr5_4:19770186-19770205 None:intergenic 35.0%
TTCATGGTCAAAAGCTTCAA+AGG - chr5_4:19770232-19770251 Msa0842630:intron 35.0%
TTGTGATATATGGCTGTGTT+TGG - chr5_4:19770347-19770366 Msa0842630:intron 35.0%
CTATAAGCTCAAAAGCTACT+TGG + chr5_4:19770493-19770512 None:intergenic 35.0%
!!! GGGTAGTTATTTCTTTTACG+TGG + chr5_4:19770582-19770601 None:intergenic 35.0%
CAGAATTAGAAGCTCTTTGT+TGG + chr5_4:19771440-19771459 None:intergenic 35.0%
AAAAGTGGTGAATGTGTGAA+GGG + chr5_4:19771539-19771558 None:intergenic 35.0%
TGAATAGCATCATGTTAGCT+TGG + chr5_4:19771596-19771615 None:intergenic 35.0%
! GAAATTGGTGGCTTGAATTT+GGG - chr5_4:19771729-19771748 Msa0842630:CDS 35.0%
CATGCAAGTATGGTACAATT+TGG - chr5_4:19771806-19771825 Msa0842630:CDS 35.0%
TCACACACTTCAACTCAAAT+GGG - chr5_4:19771857-19771876 Msa0842630:CDS 35.0%
! CTTTAGGAATTTGCAAGTTG+TGG - chr5_4:19771922-19771941 Msa0842630:CDS 35.0%
GAATTTGCAAGTTGTGGATT+GGG - chr5_4:19771928-19771947 Msa0842630:exon 35.0%
AGAACCCTTAGATTTGACAA+GGG + chr5_4:19771968-19771987 None:intergenic 35.0%
TAGAACCCTTAGATTTGACA+AGG + chr5_4:19771969-19771988 None:intergenic 35.0%
CCTTGTATGTCATAGCAATT+TGG + chr5_4:19772001-19772020 None:intergenic 35.0%
CCAAATTGCTATGACATACA+AGG - chr5_4:19771998-19772017 Msa0842630:five_prime_UTR 35.0%
AATTGCTATGACATACAAGG+AGG - chr5_4:19772001-19772020 Msa0842630:five_prime_UTR 35.0%
!! TGGGGGAATTACTTTTACTA+TGG - chr5_4:19772037-19772056 Msa0842630:five_prime_UTR 35.0%
!! GGGAATTACTTTTACTATGG+TGG - chr5_4:19772040-19772059 Msa0842630:five_prime_UTR 35.0%
AGAAATGTGAAATGTCCCTA+AGG - chr5_4:19772070-19772089 Msa0842630:five_prime_UTR 35.0%
GAAATGTGAAATGTCCCTAA+GGG - chr5_4:19772071-19772090 Msa0842630:five_prime_UTR 35.0%
!! GTATTCAAGTTTTGCCCATG+TGG + chr5_4:19767139-19767158 None:intergenic 40.0%
!!! CCTAGATTTTGATTGTGGCA+AGG + chr5_4:19767400-19767419 None:intergenic 40.0%
CCTTGCCACAATCAAAATCT+AGG - chr5_4:19767397-19767416 Msa0842630:CDS 40.0%
AGGAGGAAAAAACAGGACAA+AGG + chr5_4:19767600-19767619 None:intergenic 40.0%
CATTCTATGATATCACCGTC+AGG + chr5_4:19767870-19767889 None:intergenic 40.0%
GTTAAAAGGACAAAGACCAC+TGG - chr5_4:19767929-19767948 Msa0842630:intron 40.0%
GGCCTAAAACTCATAACAAC+AGG - chr5_4:19768082-19768101 Msa0842630:intron 40.0%
! TTTTCAGTCATGGAGCTTGT+CGG - chr5_4:19768122-19768141 Msa0842630:intron 40.0%
CTGATTGGAATTGTTCCTTC+CGG + chr5_4:19768159-19768178 None:intergenic 40.0%
TGTTCCGTTGTTCTTCTGAT+TGG + chr5_4:19768174-19768193 None:intergenic 40.0%
CAACGGAACAAGACATGTTA+AGG - chr5_4:19768184-19768203 Msa0842630:intron 40.0%
CACAAATAGCAATGGACATG+AGG - chr5_4:19768260-19768279 Msa0842630:intron 40.0%
! TTTTTCCTTGCAAAGGCTCA+TGG + chr5_4:19768724-19768743 None:intergenic 40.0%
TGATCACTGACACTTCAGAT+AGG - chr5_4:19768796-19768815 Msa0842630:intron 40.0%
ATAGTGACACTTCAGAGTGA+AGG - chr5_4:19768950-19768969 Msa0842630:intron 40.0%
ACCTGACATATATCGGATGT+CGG + chr5_4:19768981-19769000 None:intergenic 40.0%
ATTGTTGTACAGCATGCAGT+TGG - chr5_4:19769121-19769140 Msa0842630:intron 40.0%
AATTCAGCTTGTCCCAAATG+TGG - chr5_4:19769221-19769240 Msa0842630:intron 40.0%
ATTCAGCTTGTCCCAAATGT+GGG - chr5_4:19769222-19769241 Msa0842630:intron 40.0%
ACCAGAAATGACCCACATTT+GGG + chr5_4:19769236-19769255 None:intergenic 40.0%
AACCAGAAATGACCCACATT+TGG + chr5_4:19769237-19769256 None:intergenic 40.0%
!! TGGGTCATTTCTGGTTCATT+TGG - chr5_4:19769241-19769260 Msa0842630:intron 40.0%
GATCTTAACACCATTGAAGC+TGG - chr5_4:19769268-19769287 Msa0842630:intron 40.0%
TTAACACCATTGAAGCTGGT+TGG - chr5_4:19769272-19769291 Msa0842630:intron 40.0%
CTTCCTTACACTTCAAGCAA+AGG + chr5_4:19769653-19769672 None:intergenic 40.0%
AGTGATGCATATCAAGCAAC+TGG - chr5_4:19769725-19769744 Msa0842630:intron 40.0%
CCAACTTCTTCATATACTGG+GGG - chr5_4:19769818-19769837 Msa0842630:intron 40.0%
AAATTAAGGGTCCCACCTAA+AGG - chr5_4:19770146-19770165 Msa0842630:intron 40.0%
GAAGCTCTTTGTTGGGAAAA+AGG + chr5_4:19771432-19771451 None:intergenic 40.0%
GAAAAGTGGTGAATGTGTGA+AGG + chr5_4:19771540-19771559 None:intergenic 40.0%
! TAGCATCATGTTAGCTTGGT+TGG + chr5_4:19771592-19771611 None:intergenic 40.0%
AGGATCCAAAGCATGGAAAT+TGG - chr5_4:19771714-19771733 Msa0842630:CDS 40.0%
ATCCAAAGCATGGAAATTGG+TGG - chr5_4:19771717-19771736 Msa0842630:CDS 40.0%
! GGAAATTGGTGGCTTGAATT+TGG - chr5_4:19771728-19771747 Msa0842630:CDS 40.0%
TGAATTTGGGTCCGGAATAT+TGG - chr5_4:19771742-19771761 Msa0842630:CDS 40.0%
CCAATACCCTACCAATATTC+CGG + chr5_4:19771756-19771775 None:intergenic 40.0%
CTTAAGGGATCATGCAAGTA+TGG - chr5_4:19771796-19771815 Msa0842630:CDS 40.0%
GCAAGTATGGTACAATTTGG+TGG - chr5_4:19771809-19771828 Msa0842630:CDS 40.0%
GTCACACACTTCAACTCAAA+TGG - chr5_4:19771856-19771875 Msa0842630:CDS 40.0%
ACTTCAACTCAAATGGGTAG+TGG - chr5_4:19771863-19771882 Msa0842630:CDS 40.0%
CAAAACCTTCTCCAGCAAAA+TGG + chr5_4:19771889-19771908 None:intergenic 40.0%
! CATTTTGCTGGAGAAGGTTT+TGG - chr5_4:19771887-19771906 Msa0842630:CDS 40.0%
GGAATTTGCAAGTTGTGGAT+TGG - chr5_4:19771927-19771946 Msa0842630:exon 40.0%
! GTCAAATCTAAGGGTTCTAG+CGG - chr5_4:19771970-19771989 Msa0842630:five_prime_UTR 40.0%
!!! TACTTTTACTATGGTGGACC+AGG - chr5_4:19772046-19772065 Msa0842630:five_prime_UTR 40.0%
GGACATTTCACATTTCTTCC+TGG + chr5_4:19772067-19772086 None:intergenic 40.0%
!!! TCCTTTTTGAAGGGCATTCA+AGG - chr5_4:19772167-19772186 Msa0842630:five_prime_UTR 40.0%
ACCTTGAATGCCCTTCAAAA+AGG + chr5_4:19772171-19772190 None:intergenic 40.0%
CATAAGAGTCCATGCAGTAA+TGG + chr5_4:19772288-19772307 None:intergenic 40.0%
! TAGGAGTGTGTACAAGGGAA+GGG + chr5_4:19767057-19767076 None:intergenic 45.0%
! ATAGGAGTGTGTACAAGGGA+AGG + chr5_4:19767058-19767077 None:intergenic 45.0%
AACTTGAGCTCTCAGGAGTT+GGG + chr5_4:19767194-19767213 None:intergenic 45.0%
TACTGACAACTTGAGCTCTC+AGG + chr5_4:19767201-19767220 None:intergenic 45.0%
!!! TGAGTTTTAGGCCTTTCTGG+AGG + chr5_4:19768075-19768094 None:intergenic 45.0%
! CGAGCGAGAATTTTCAGTCA+TGG - chr5_4:19768112-19768131 Msa0842630:intron 45.0%
TTTCAGTCATGGAGCTTGTC+GGG - chr5_4:19768123-19768142 Msa0842630:intron 45.0%
! GGTCAGATTTTCCCTACACA+AGG + chr5_4:19768673-19768692 None:intergenic 45.0%
AACTACCATGAGCCTTTGCA+AGG - chr5_4:19768716-19768735 Msa0842630:intron 45.0%
ACCGACATCCGATATATGTC+AGG - chr5_4:19768977-19768996 Msa0842630:intron 45.0%
CATGCAGTTGGATATGTGAC+TGG - chr5_4:19769133-19769152 Msa0842630:intron 45.0%
AAAGGCCAGCATAAACGTGT+GGG - chr5_4:19769174-19769193 Msa0842630:intron 45.0%
TCCCAAATGTGGGTCATTTC+TGG - chr5_4:19769232-19769251 Msa0842630:intron 45.0%
CCCCCAGTATATGAAGAAGT+TGG + chr5_4:19769821-19769840 None:intergenic 45.0%
AGCCACCAATTTCCATGCTT+TGG + chr5_4:19771722-19771741 None:intergenic 45.0%
TTTGGGTCCGGAATATTGGT+AGG - chr5_4:19771746-19771765 Msa0842630:CDS 45.0%
TTGGGTCCGGAATATTGGTA+GGG - chr5_4:19771747-19771766 Msa0842630:CDS 45.0%
CCGGAATATTGGTAGGGTAT+TGG - chr5_4:19771753-19771772 Msa0842630:CDS 45.0%
!!! CATTTTTTTTTTTTAAATAT+CGG + chr5_4:19768870-19768889 None:intergenic 5.0%
!!! ATAATTTAATTTAGTTTATT+TGG + chr5_4:19769498-19769517 None:intergenic 5.0%
!!! AAATTAAATTATTTTTTTTC+AGG - chr5_4:19769506-19769525 Msa0842630:CDS 5.0%
!!! TATTTTTTATTTTTTTATAG+TGG - chr5_4:19769949-19769968 Msa0842630:intron 5.0%
!! AAGTATTATAATTTATAAAA+CGG - chr5_4:19770018-19770037 Msa0842630:intron 5.0%
!!! TTTATTTCAAAATATTTTTA+TGG + chr5_4:19770835-19770854 None:intergenic 5.0%
!!! TTATTTTATGTTTTTTTTTT+TGG - chr5_4:19771169-19771188 Msa0842630:CDS 5.0%
! AGGAGTGTGTACAAGGGAAG+GGG + chr5_4:19767056-19767075 None:intergenic 50.0%
TATAAGGGCTCCAACCCACA+TGG - chr5_4:19767121-19767140 Msa0842630:three_prime_UTR 50.0%
ATAAGGGCTCCAACCCACAT+GGG - chr5_4:19767122-19767141 Msa0842630:three_prime_UTR 50.0%
! CAAGTTTTGCCCATGTGGGT+TGG + chr5_4:19767134-19767153 None:intergenic 50.0%
CAACTTGAGCTCTCAGGAGT+TGG + chr5_4:19767195-19767214 None:intergenic 50.0%
CTTGTCGGGTGAATCATGTC+CGG - chr5_4:19768137-19768156 Msa0842630:intron 50.0%
CAAAGGCCAGCATAAACGTG+TGG - chr5_4:19769173-19769192 Msa0842630:intron 50.0%
AACTGTCCCCATAGAGCTCA+GGG + chr5_4:19769536-19769555 None:intergenic 50.0%
TAACTGTCCCCATAGAGCTC+AGG + chr5_4:19769537-19769556 None:intergenic 50.0%
! TGGTAGGGTCTCCGTTGTTT+TGG - chr5_4:19770302-19770321 Msa0842630:intron 50.0%
TGGTGGCTTGAATTTGGGTC+CGG - chr5_4:19771734-19771753 Msa0842630:CDS 50.0%
!! ATGGGTAGTGGCCATTTTGC+TGG - chr5_4:19771875-19771894 Msa0842630:CDS 50.0%
!! AGTGGCCATTTTGCTGGAGA+AGG - chr5_4:19771881-19771900 Msa0842630:CDS 50.0%
!! TGAAGGGCATTCAAGGTGCT+TGG - chr5_4:19772174-19772193 Msa0842630:five_prime_UTR 50.0%
!! GAAGGGCATTCAAGGTGCTT+GGG - chr5_4:19772175-19772194 Msa0842630:five_prime_UTR 50.0%
!! AAGGGCATTCAAGGTGCTTG+GGG - chr5_4:19772176-19772195 Msa0842630:five_prime_UTR 50.0%
TTGAGCTCTCAGGAGTTGGG+TGG + chr5_4:19767191-19767210 None:intergenic 55.0%
GTGTGCAGGATCCTCCAGAA+AGG - chr5_4:19768061-19768080 Msa0842630:intron 55.0%
TCGGGTGAATCATGTCCGGA+AGG - chr5_4:19768141-19768160 Msa0842630:intron 55.0%
CACCATTGAAGCTGGTTGGC+AGG - chr5_4:19769276-19769295 Msa0842630:intron 55.0%
TGCCTGCCAACCAGCTTCAA+TGG + chr5_4:19769281-19769300 None:intergenic 55.0%
AGGTCAGCCCTGAGCTCTAT+GGG - chr5_4:19769526-19769545 Msa0842630:CDS 55.0%
TGGGGCCCACACGTTTATGC+TGG + chr5_4:19769182-19769201 None:intergenic 60.0%
CAGGTCAGCCCTGAGCTCTA+TGG - chr5_4:19769525-19769544 Msa0842630:CDS 60.0%
GGTCAGCCCTGAGCTCTATG+GGG - chr5_4:19769527-19769546 Msa0842630:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 19766981 19772349 19766981 ID=Msa0842630;Name=Msa0842630
chr5_4 mRNA 19766981 19772349 19766981 ID=Msa0842630-mRNA-1;Parent=Msa0842630;Name=Msa0842630-mRNA-1;_AED=0.15;_eAED=0.15;_QI=407|0.8|1|1|1|1|6|259|409
chr5_4 exon 19766981 19767614 19766981 ID=Msa0842630-mRNA-1:exon:8226;Parent=Msa0842630-mRNA-1
chr5_4 exon 19769459 19769605 19769459 ID=Msa0842630-mRNA-1:exon:8225;Parent=Msa0842630-mRNA-1
chr5_4 exon 19769749 19769802 19769749 ID=Msa0842630-mRNA-1:exon:8224;Parent=Msa0842630-mRNA-1
chr5_4 exon 19771049 19771261 19771049 ID=Msa0842630-mRNA-1:exon:8223;Parent=Msa0842630-mRNA-1
chr5_4 exon 19771380 19771466 19771380 ID=Msa0842630-mRNA-1:exon:8222;Parent=Msa0842630-mRNA-1
chr5_4 exon 19771589 19772349 19771589 ID=Msa0842630-mRNA-1:exon:8221;Parent=Msa0842630-mRNA-1
chr5_4 five_prime_UTR 19771943 19772349 19771943 ID=Msa0842630-mRNA-1:five_prime_utr;Parent=Msa0842630-mRNA-1
chr5_4 CDS 19771589 19771942 19771589 ID=Msa0842630-mRNA-1:cds;Parent=Msa0842630-mRNA-1
chr5_4 CDS 19771380 19771466 19771380 ID=Msa0842630-mRNA-1:cds;Parent=Msa0842630-mRNA-1
chr5_4 CDS 19771049 19771261 19771049 ID=Msa0842630-mRNA-1:cds;Parent=Msa0842630-mRNA-1
chr5_4 CDS 19769749 19769802 19769749 ID=Msa0842630-mRNA-1:cds;Parent=Msa0842630-mRNA-1
chr5_4 CDS 19769459 19769605 19769459 ID=Msa0842630-mRNA-1:cds;Parent=Msa0842630-mRNA-1
chr5_4 CDS 19767240 19767614 19767240 ID=Msa0842630-mRNA-1:cds;Parent=Msa0842630-mRNA-1
chr5_4 three_prime_UTR 19766981 19767239 19766981 ID=Msa0842630-mRNA-1:three_prime_utr;Parent=Msa0842630-mRNA-1
Gene Sequence

>Msa0842630

ATGTGTTTCCCTTGCCACAATCAAAATCTAGGAACACACAACAATTTTGTTTTTGTAGATGGAAGAAGAAGACAAGATCAATGTGAACTACTTCAAACGGATTCTTCAATAGAAACAAAGAAAGGAAAATTCTCAAGCATGGATTTTAGCTTGTGTTTGACTTCTCCAATCATTTACAATCTTGTACATTTTCTTCTTGTTTTTTCTTTCCTTTGTCCTGTTTTTTCCTCCTCAAAAATTGACAACAACCCTGTTGCCAATAACACTTTTAAACCAAAGGAAGAATTGTTCAAACTAAACATCATAAGAAATCGTCTTCAACAGATCAATAAACCTTCTATTAAGACAATTCAGAGTCCTGACGGTGATATCATAGAATGTGTTGCGTCTCATCAACAACCAGCTTTTGATCATCCTATGTTAAAAGGACAAAGACCACTGGATCCTCCAGAAAGGCCTAAAACTCATAACAACAGGGATGTGTCGAGCGAGAATTTTCAGTCATGGAGCTTGTCGGGTGAATCATGTCCGGAAGGAACAATTCCAATCAGAAGAACAACGGAACAAGACATGTTAAGGGCTAGTTCTATCAATACATTTGGAAGAAAATTAAGAAGACGTGTTAGAAGAGACACAAATAGCAATGGACATGAGGTCAGCCCTGAGCTCTATGGGGACAGTTACCCTAGATTCTTTACTTATTGGACGAGTGATGCATATCAAGCAACTGGATGCTACAATTTATTGTGTTCTGGCTTTGTTCAAACTAACAATAAAATTGCAATTGGTGCTGCAATCTCTCCAACTTCTTCATATACTGGGGGCCAATTTGATATTAGCTTGCTCATTTGGAAGGATCCAAAGCATGGAAATTGGTGGCTTGAATTTGGGTCCGGAATATTGGTAGGGTATTGGCCATCGTTTTTGTTTACACACTTAAGGGATCATGCAAGTATGGTACAATTTGGTGGAGAAATTGTGAATTCAAGACAAGGGTCACACACTTCAACTCAAATGGGTAGTGGCCATTTTGCTGGAGAAGGTTTTGGAAAAGCATCATACTTTAGGAATTTGCAAGTTGTGGATTGGGATAACAATTTAATACCCTTGTCAAATCTAAGGGTTCTAGCGGATCATCCAAATTGCTATGACATACAAGGAGGAATAAATAATGTTTGGGGGAATTACTTTTACTATGGTGGACCAGGAAGAAATGTGAAATGTCCCTAA
Protein sequence

>Msa0842630

MCFPCHNQNLGTHNNFVFVDGRRRQDQCELLQTDSSIETKKGKFSSMDFSLCLTSPIIYNLVHFLLVFSFLCPVFSSSKIDNNPVANNTFKPKEELFKLNIIRNRLQQINKPSIKTIQSPDGDIIECVASHQQPAFDHPMLKGQRPLDPPERPKTHNNRDVSSENFQSWSLSGESCPEGTIPIRRTTEQDMLRASSINTFGRKLRRRVRRDTNSNGHEVSPELYGDSYPRFFTYWTSDAYQATGCYNLLCSGFVQTNNKIAIGAAISPTSSYTGGQFDISLLIWKDPKHGNWWLEFGSGILVGYWPSFLFTHLRDHASMVQFGGEIVNSRQGSHTSTQMGSGHFAGEGFGKASYFRNLQVVDWDNNLIPLSNLRVLADHPNCYDIQGGINNVWGNYFYYGGPGRNVKCP*