AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0853710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTATGTGACTTTCTCTTT+TGG 0.274538 5_4:+45669457 Msa0853710:five_prime_UTR
CGTTGATTGCCCAACATTAG+AGG 0.310931 5_4:+45668954 Msa0853710:five_prime_UTR
ATTAAAGCAGCTGCTAAAAC+TGG 0.345489 5_4:+45669941 Msa0853710:intron
ATTAGAGAGCCTACCAATTC+AGG 0.345911 5_4:-45669326 None:intergenic
TAAAGGATTGCGATGCTTGC+CGG 0.362783 5_4:+45668891 None:intergenic
TTTCTTGGTAGTCTCTTTGT+AGG 0.369841 5_4:+45669400 Msa0853710:intron
AGAGGGAACAGGGGAGGATT+GGG 0.384073 5_4:+45668987 Msa0853710:five_prime_UTR
GAACGATGCAAAGAGGGAAC+AGG 0.385790 5_4:+45668976 Msa0853710:five_prime_UTR
TGCCACATTTCCACCTGAAT+TGG 0.386401 5_4:+45669313 Msa0853710:five_prime_UTR
CAGTTCTCATTCTTCGGAAA+GGG 0.386986 5_4:-45669772 None:intergenic
TTGCATCGTTCCTCTAATGT+TGG 0.397864 5_4:-45668964 None:intergenic
TGCATCGTTCCTCTAATGTT+GGG 0.398540 5_4:-45668963 None:intergenic
AAGAGGGAACAGGGGAGGAT+TGG 0.409457 5_4:+45668986 Msa0853710:five_prime_UTR
GCTCTCCAGTTCTCATTCTT+CGG 0.411506 5_4:-45669778 None:intergenic
CGAGGCTCATGAACAGTTCA+TGG 0.414050 5_4:+45671495 Msa0853710:CDS
ATAGGTAATGAACAGTTAGA+TGG 0.426662 5_4:+45670714 Msa0853710:CDS
CCAAAGTCAAGAGACATTAG+AGG 0.440154 5_4:-45669428 None:intergenic
CCTCTAATGTCTCTTGACTT+TGG 0.442600 5_4:+45669428 Msa0853710:five_prime_UTR
CCAGTTCTCATTCTTCGGAA+AGG 0.445257 5_4:-45669773 None:intergenic
TATGATAAAAGCCAAGCTTC+CGG 0.445918 5_4:+45670031 Msa0853710:CDS
CCTTTCCGAAGAATGAGAAC+TGG 0.464369 5_4:+45669773 Msa0853710:five_prime_UTR
ATGTACTTGAAGTGAATATC+CGG 0.482581 5_4:-45670050 None:intergenic
TTATTTGTATATTTCCCCTA+AGG 0.501306 5_4:-45669824 None:intergenic
AACTGTTCATGAGCCTCGAC+TGG 0.502751 5_4:-45671490 None:intergenic
AAATATACAAATAATTGTGC+AGG 0.513258 5_4:+45669833 Msa0853710:five_prime_UTR
TTCACTTCAAGTACATTGAA+AGG 0.527519 5_4:+45670057 Msa0853710:CDS
AATATTGATACAAACTCAGT+TGG 0.540842 5_4:+45671460 Msa0853710:CDS
GAAGTGAATATCCGGAAGCT+TGG 0.545117 5_4:-45670042 None:intergenic
AACGATGCAAAGAGGGAACA+GGG 0.568607 5_4:+45668977 Msa0853710:five_prime_UTR
ATGGAACAGCAGCGGCATGT+TGG 0.580537 5_4:+45671514 Msa0853710:CDS
TACCAATTCAGGTGGAAATG+TGG 0.602730 5_4:-45669315 None:intergenic
AGTTGGTTCTGAACCAGTCG+AGG 0.608872 5_4:+45671477 Msa0853710:CDS
TTAGAGGAACGATGCAAAGA+GGG 0.615804 5_4:+45668970 Msa0853710:five_prime_UTR
GTGAGGTGTCGAATACCCTC+CGG 0.623261 5_4:-45668910 None:intergenic
AGGATTGCGATGCTTGCCGG+AGG 0.627574 5_4:+45668894 None:intergenic
ATTAGAGGAACGATGCAAAG+AGG 0.636912 5_4:+45668969 Msa0853710:five_prime_UTR
AAAGAGAAAGTCACATAACA+AGG 0.638639 5_4:-45669454 None:intergenic
AACAGTTCATGGAACAGCAG+CGG 0.639450 5_4:+45671506 Msa0853710:CDS
TCAGAAGCTCAAGAGAAGTG+AGG 0.641140 5_4:-45668927 None:intergenic
ATGCAAAGAGGGAACAGGGG+AGG 0.644478 5_4:+45668981 Msa0853710:five_prime_UTR
AGAGAGCCTACCAATTCAGG+TGG 0.651833 5_4:-45669323 None:intergenic
ACGATGCAAAGAGGGAACAG+GGG 0.655349 5_4:+45668978 Msa0853710:five_prime_UTR
GGATTGCGATGCTTGCCGGA+GGG 0.660517 5_4:+45668895 None:intergenic
ATGTGTAGAGAATGTGACAT+GGG 0.663555 5_4:+45669972 Msa0853710:five_prime_UTR
AATGTGTAGAGAATGTGACA+TGG 0.746081 5_4:+45669971 Msa0853710:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATATGAATTTATACA+CGG - chr5_4:45671379-45671398 None:intergenic 10.0%
!!! TTTTTTTTTAATTTTGCTTC+TGG + chr5_4:45669288-45669307 Msa0853710:intron 15.0%
!! GAAATATAATCTTAGAAAAA+TGG - chr5_4:45670142-45670161 None:intergenic 15.0%
!! ACTAAAATGAGAAAATAATA+TGG - chr5_4:45670328-45670347 None:intergenic 15.0%
!! AAAAATTGTTTATAAGGAAT+TGG + chr5_4:45670913-45670932 Msa0853710:intron 15.0%
!!! GATCAACGAATATATATTTT+TGG - chr5_4:45669030-45669049 None:intergenic 20.0%
!! ACAGTAAAAAAGAATAAAGA+CGG - chr5_4:45669358-45669377 None:intergenic 20.0%
!!! TGAATATCCCTTTTTTATTT+GGG + chr5_4:45669541-45669560 Msa0853710:intron 20.0%
!! AAATATACAAATAATTGTGC+AGG + chr5_4:45669833-45669852 Msa0853710:five_prime_UTR 20.0%
!! CTTATTCATACCAAAAAAAA+TGG + chr5_4:45670654-45670673 Msa0853710:intron 20.0%
!!! GTTATTTGAGGTATTAAAAA+AGG + chr5_4:45670761-45670780 Msa0853710:intron 20.0%
!! GAGCAAAAAAATTGTTTATA+AGG + chr5_4:45670907-45670926 Msa0853710:intron 20.0%
!! GAGTAAGTAATAATTGAATA+TGG + chr5_4:45670939-45670958 Msa0853710:intron 20.0%
!!! AGTAAAATTGATTTTTGCGA+TGG - chr5_4:45669064-45669083 None:intergenic 25.0%
!! ATCGCAAAAATCAATTTTAC+TGG + chr5_4:45669063-45669082 Msa0853710:intron 25.0%
! TTGAAATCCAAACATAAGAA+GGG - chr5_4:45669104-45669123 None:intergenic 25.0%
! AAAATATCTTTCATGTACTC+TGG - chr5_4:45669197-45669216 None:intergenic 25.0%
! AAGGATAAATGTTCTAATTC+AGG + chr5_4:45669232-45669251 Msa0853710:intron 25.0%
! TAAAAAAGAATAAAGACGGA+GGG - chr5_4:45669354-45669373 None:intergenic 25.0%
! CAAATAAAAAAGGGATATTC+AGG - chr5_4:45669542-45669561 None:intergenic 25.0%
!!! CTGAATATCCCTTTTTTATT+TGG + chr5_4:45669540-45669559 Msa0853710:intron 25.0%
!!! GAATATCCCTTTTTTATTTG+GGG + chr5_4:45669542-45669561 Msa0853710:intron 25.0%
! TTTCACAAAGTTGAGAAATA+TGG - chr5_4:45669618-45669637 None:intergenic 25.0%
! TATTTCTCAACTTTGTGAAA+AGG + chr5_4:45669618-45669637 Msa0853710:intron 25.0%
!! ATCTTTTATTGGTAAACCTT+AGG + chr5_4:45669808-45669827 Msa0853710:five_prime_UTR 25.0%
!! TCTTTTATTGGTAAACCTTA+GGG + chr5_4:45669809-45669828 Msa0853710:five_prime_UTR 25.0%
! TTATTTGTATATTTCCCCTA+AGG - chr5_4:45669827-45669846 None:intergenic 25.0%
! ATGTAATTGAAGTGAATATC+AGG - chr5_4:45669923-45669942 None:intergenic 25.0%
!! ACATTGAAAGGTAAAGTTTT+TGG + chr5_4:45670069-45670088 Msa0853710:intron 25.0%
! AGATTATATTTCTTGCTTCT+AGG + chr5_4:45670150-45670169 Msa0853710:intron 25.0%
! GAGAAAATAATATGGTCAAA+AGG - chr5_4:45670320-45670339 None:intergenic 25.0%
!!! ATTGAACATATTTTGAGTGA+GGG + chr5_4:45670385-45670404 Msa0853710:intron 25.0%
! CAAAGATATAAATGTGTGTA+AGG + chr5_4:45670411-45670430 Msa0853710:intron 25.0%
!!! CTAAACTGAACCATTTTTTT+TGG - chr5_4:45670667-45670686 None:intergenic 25.0%
!! TTACCATCTTTTAAATGTAC+AGG + chr5_4:45670688-45670707 Msa0853710:intron 25.0%
!!! TTTTAAATGTACAGGTTGAT+AGG + chr5_4:45670696-45670715 Msa0853710:intron 25.0%
!!! TAAACAATTTTTTTGCTCTG+TGG - chr5_4:45670905-45670924 None:intergenic 25.0%
! AATTGAATATGGTTAAACCA+TGG + chr5_4:45670950-45670969 Msa0853710:intron 25.0%
!!! TTATGTGCCACTATAAATTT+TGG + chr5_4:45671017-45671036 Msa0853710:intron 25.0%
!! TTGATACTATGATTTTGCTT+TGG + chr5_4:45671071-45671090 Msa0853710:intron 25.0%
!! TGATACTATGATTTTGCTTT+GGG + chr5_4:45671072-45671091 Msa0853710:intron 25.0%
! TAGCACAATCATGAAATTTA+TGG + chr5_4:45671200-45671219 Msa0853710:intron 25.0%
! AATATTGATACAAACTCAGT+TGG + chr5_4:45671460-45671479 Msa0853710:CDS 25.0%
TGAAATCCAAACATAAGAAG+GGG - chr5_4:45669103-45669122 None:intergenic 30.0%
GTTGAAATCCAAACATAAGA+AGG - chr5_4:45669105-45669124 None:intergenic 30.0%
TTAGAACATTTATCCTTCTG+AGG - chr5_4:45669229-45669248 None:intergenic 30.0%
GTAAAAAAGAATAAAGACGG+AGG - chr5_4:45669355-45669374 None:intergenic 30.0%
AAAGAGAAAGTCACATAACA+AGG - chr5_4:45669457-45669476 None:intergenic 30.0%
! TGTTATGTGACTTTCTCTTT+TGG + chr5_4:45669457-45669476 Msa0853710:five_prime_UTR 30.0%
TTACTGCACTATAAGAAGTA+AGG - chr5_4:45669514-45669533 None:intergenic 30.0%
TCAATGCCCCAAATAAAAAA+GGG - chr5_4:45669551-45669570 None:intergenic 30.0%
TTCAATGCCCCAAATAAAAA+AGG - chr5_4:45669552-45669571 None:intergenic 30.0%
ATCAACTTAAATAGCCCTAA+TGG - chr5_4:45669651-45669670 None:intergenic 30.0%
!! ATTAGGGCTATTTAAGTTGA+TGG + chr5_4:45669650-45669669 Msa0853710:intron 30.0%
CAAAACATTGTTGAATTCGT+TGG + chr5_4:45669715-45669734 Msa0853710:intron 30.0%
! GAGCATGAAAGATCTTTTAT+TGG + chr5_4:45669797-45669816 Msa0853710:five_prime_UTR 30.0%
! CTTTTATTGGTAAACCTTAG+GGG + chr5_4:45669810-45669829 Msa0853710:five_prime_UTR 30.0%
AATTGTGCAGGTAATGTATT+AGG + chr5_4:45669845-45669864 Msa0853710:intron 30.0%
ATGTACTTGAAGTGAATATC+CGG - chr5_4:45670053-45670072 None:intergenic 30.0%
TTCACTTCAAGTACATTGAA+AGG + chr5_4:45670057-45670076 Msa0853710:CDS 30.0%
!!! AAAGGTAAAGTTTTTGGTTG+CGG + chr5_4:45670075-45670094 Msa0853710:intron 30.0%
ACAAATTCTGCTATAGTTGT+TGG + chr5_4:45670181-45670200 Msa0853710:intron 30.0%
CAAATTCTGCTATAGTTGTT+GGG + chr5_4:45670182-45670201 Msa0853710:intron 30.0%
TCTTATTCCCATTGAATCAT+TGG + chr5_4:45670239-45670258 Msa0853710:intron 30.0%
TCTTCAACCAATGATTCAAT+GGG - chr5_4:45670249-45670268 None:intergenic 30.0%
TTCTTCAACCAATGATTCAA+TGG - chr5_4:45670250-45670269 None:intergenic 30.0%
!!! TCATTTTAGTTTTTCTGTGC+AGG + chr5_4:45670338-45670357 Msa0853710:intron 30.0%
!! GATTGAACATATTTTGAGTG+AGG + chr5_4:45670384-45670403 Msa0853710:intron 30.0%
AAGGAGCTTAATCAACTAAT+GGG - chr5_4:45670472-45670491 None:intergenic 30.0%
AGCTTCAATTTGCCAAATTT+AGG + chr5_4:45670525-45670544 Msa0853710:intron 30.0%
GCTTCAATTTGCCAAATTTA+GGG + chr5_4:45670526-45670545 Msa0853710:intron 30.0%
!! ATAAGAAAGCACCCTAAATT+TGG - chr5_4:45670540-45670559 None:intergenic 30.0%
GAACGAAAGTAAATTGTTTC+TGG - chr5_4:45670613-45670632 None:intergenic 30.0%
TTTCGTTCACATGATGTATT+TGG + chr5_4:45670625-45670644 Msa0853710:intron 30.0%
CAACCTGTACATTTAAAAGA+TGG - chr5_4:45670694-45670713 None:intergenic 30.0%
ATAGGTAATGAACAGTTAGA+TGG + chr5_4:45670714-45670733 Msa0853710:CDS 30.0%
!!! GGTTTTGAATATTTTGTGCA+AGG + chr5_4:45670735-45670754 Msa0853710:CDS 30.0%
AGCACATCCAAAATTTATAG+TGG - chr5_4:45671027-45671046 None:intergenic 30.0%
! GATACTATGATTTTGCTTTC+CGG + chr5_4:45671177-45671196 Msa0853710:intron 30.0%
AAATTTCATGATTGTGCTAC+CGG - chr5_4:45671199-45671218 None:intergenic 30.0%
TGAGAGTTATTATGGTACAA+AGG - chr5_4:45671304-45671323 None:intergenic 30.0%
TGCAATTGTGAGAGTTATTA+TGG - chr5_4:45671312-45671331 None:intergenic 30.0%
!!! ATTTGTAGTGTTTTTTCACG+AGG + chr5_4:45671435-45671454 Msa0853710:intron 30.0%
CTATTTCCCCTTCTTATGTT+TGG + chr5_4:45669094-45669113 Msa0853710:intron 35.0%
! TTTTACTGTTCAACCTCAGA+AGG + chr5_4:45669213-45669232 Msa0853710:intron 35.0%
ACAGTGCAGTAACAATTTCT+TGG + chr5_4:45669385-45669404 Msa0853710:intron 35.0%
! TTTCTTGGTAGTCTCTTTGT+AGG + chr5_4:45669400-45669419 Msa0853710:intron 35.0%
!! CTTTCTCTTTTGGCTATCAT+AGG + chr5_4:45669467-45669486 Msa0853710:five_prime_UTR 35.0%
TTGGCTATCATAGGTAAGTT+TGG + chr5_4:45669476-45669495 Msa0853710:intron 35.0%
!! TGGGGCATTGAAATATGTAA+TGG + chr5_4:45669560-45669579 Msa0853710:intron 35.0%
AACTTAAATAGCCCTAATGG+AGG - chr5_4:45669648-45669667 None:intergenic 35.0%
! AATTCGTTGGAGTGATTTTC+AGG + chr5_4:45669728-45669747 Msa0853710:intron 35.0%
! ACTTGCTGCTCAAAATGAAA+AGG - chr5_4:45669893-45669912 None:intergenic 35.0%
ATTAAAGCAGCTGCTAAAAC+TGG + chr5_4:45669941-45669960 Msa0853710:intron 35.0%
AATGTGTAGAGAATGTGACA+TGG + chr5_4:45669971-45669990 Msa0853710:five_prime_UTR 35.0%
ATGTGTAGAGAATGTGACAT+GGG + chr5_4:45669972-45669991 Msa0853710:five_prime_UTR 35.0%
TATGATAAAAGCCAAGCTTC+CGG + chr5_4:45670031-45670050 Msa0853710:CDS 35.0%
AGGAGCTTAATCAACTAATG+GGG - chr5_4:45670471-45670490 None:intergenic 35.0%
GAAGGAGCTTAATCAACTAA+TGG - chr5_4:45670473-45670492 None:intergenic 35.0%
CAAATTGAAGCTTCAACCAA+AGG - chr5_4:45670517-45670536 None:intergenic 35.0%
TATAGTGGCACATAATCACT+TGG - chr5_4:45671012-45671031 None:intergenic 35.0%
!!! GTCACTATTGTTGTTTTGCT+CGG + chr5_4:45671262-45671281 Msa0853710:intron 35.0%
!!! TCACTATTGTTGTTTTGCTC+GGG + chr5_4:45671263-45671282 Msa0853710:intron 35.0%
TTGCACACAAAGAATCAGTT+TGG + chr5_4:45671327-45671346 Msa0853710:intron 35.0%
CACTACAAATTTGTGAGAGA+AGG - chr5_4:45671425-45671444 None:intergenic 35.0%
TGCATCGTTCCTCTAATGTT+GGG - chr5_4:45668966-45668985 None:intergenic 40.0%
TTGCATCGTTCCTCTAATGT+TGG - chr5_4:45668967-45668986 None:intergenic 40.0%
ATTAGAGGAACGATGCAAAG+AGG + chr5_4:45668969-45668988 Msa0853710:five_prime_UTR 40.0%
TTAGAGGAACGATGCAAAGA+GGG + chr5_4:45668970-45668989 Msa0853710:five_prime_UTR 40.0%
! GATTTTTGCGATGGTGATTG+AGG - chr5_4:45669055-45669074 None:intergenic 40.0%
TACCAATTCAGGTGGAAATG+TGG - chr5_4:45669318-45669337 None:intergenic 40.0%
ACATTTCCACCTGAATTGGT+AGG + chr5_4:45669317-45669336 Msa0853710:intron 40.0%
ATTAGAGAGCCTACCAATTC+AGG - chr5_4:45669329-45669348 None:intergenic 40.0%
CCAAAGTCAAGAGACATTAG+AGG - chr5_4:45669431-45669450 None:intergenic 40.0%
CCTCTAATGTCTCTTGACTT+TGG + chr5_4:45669428-45669447 Msa0853710:five_prime_UTR 40.0%
TGAAAAGGCTTTCCTCCATT+AGG + chr5_4:45669633-45669652 Msa0853710:intron 40.0%
GAAAAGGCTTTCCTCCATTA+GGG + chr5_4:45669634-45669653 Msa0853710:intron 40.0%
CAGTTCTCATTCTTCGGAAA+GGG - chr5_4:45669775-45669794 None:intergenic 40.0%
TGGTTGAAGAATGTGAGAAG+AGG + chr5_4:45670259-45670278 Msa0853710:intron 40.0%
GGAGCTTAATCAACTAATGG+GGG - chr5_4:45670470-45670489 None:intergenic 40.0%
AATTGTGCAGCACACATTGA+AGG - chr5_4:45670491-45670510 None:intergenic 40.0%
TGTGCAAGGTGAGTTATTTG+AGG + chr5_4:45670749-45670768 Msa0853710:intron 40.0%
! AGGTAGATTTTGCACTTCCA+TGG - chr5_4:45670970-45670989 None:intergenic 40.0%
AATCTACCTCCAGTTGGAAT+TGG + chr5_4:45670981-45671000 Msa0853710:intron 40.0%
TTGCTATATCACATCAGTGC+TGG + chr5_4:45671130-45671149 Msa0853710:intron 40.0%
!!! CACTATTGTTGTTTTGCTCG+GGG + chr5_4:45671264-45671283 Msa0853710:intron 40.0%
TCAGAAGCTCAAGAGAAGTG+AGG - chr5_4:45668930-45668949 None:intergenic 45.0%
! CGTTGATTGCCCAACATTAG+AGG + chr5_4:45668954-45668973 Msa0853710:five_prime_UTR 45.0%
AACGATGCAAAGAGGGAACA+GGG + chr5_4:45668977-45668996 Msa0853710:five_prime_UTR 45.0%
!! CTCGTCGTTGTTGACTTCTT+TGG + chr5_4:45669149-45669168 Msa0853710:intron 45.0%
TGCCACATTTCCACCTGAAT+TGG + chr5_4:45669313-45669332 Msa0853710:five_prime_UTR 45.0%
CCAGTTCTCATTCTTCGGAA+AGG - chr5_4:45669776-45669795 None:intergenic 45.0%
CCTTTCCGAAGAATGAGAAC+TGG + chr5_4:45669773-45669792 Msa0853710:five_prime_UTR 45.0%
GCTCTCCAGTTCTCATTCTT+CGG - chr5_4:45669781-45669800 None:intergenic 45.0%
GAAGTGAATATCCGGAAGCT+TGG - chr5_4:45670045-45670064 None:intergenic 45.0%
CAAAAGGACATGCACATGCA+GGG - chr5_4:45670304-45670323 None:intergenic 45.0%
TCAAAAGGACATGCACATGC+AGG - chr5_4:45670305-45670324 None:intergenic 45.0%
GTGCAAAATCTACCTCCAGT+TGG + chr5_4:45670975-45670994 Msa0853710:intron 45.0%
TTGGAAGGACCAATTCCAAC+TGG - chr5_4:45670993-45671012 None:intergenic 45.0%
GTGGCACATAATCACTTGGA+AGG - chr5_4:45671008-45671027 None:intergenic 45.0%
! CAGTTTGGCCTGTTAGCTTA+CGG + chr5_4:45671342-45671361 Msa0853710:intron 45.0%
AACAGTTCATGGAACAGCAG+CGG + chr5_4:45671506-45671525 Msa0853710:CDS 45.0%
GAACGATGCAAAGAGGGAAC+AGG + chr5_4:45668976-45668995 Msa0853710:five_prime_UTR 50.0%
ACGATGCAAAGAGGGAACAG+GGG + chr5_4:45668978-45668997 Msa0853710:five_prime_UTR 50.0%
!! TTCTTTGGAACCCATTCCCC+AGG + chr5_4:45669164-45669183 Msa0853710:intron 50.0%
TGTACTCTGGATCTCTGCCT+GGG - chr5_4:45669184-45669203 None:intergenic 50.0%
ATGTACTCTGGATCTCTGCC+TGG - chr5_4:45669185-45669204 None:intergenic 50.0%
AGAGAGCCTACCAATTCAGG+TGG - chr5_4:45669326-45669345 None:intergenic 50.0%
ATGTGTGTAAGGCTCTGCAG+TGG + chr5_4:45670422-45670441 Msa0853710:intron 50.0%
GTGCTGCACAATTCTGCCTT+TGG + chr5_4:45670498-45670517 Msa0853710:intron 50.0%
GAAGGACCAATTCCAACTGG+AGG - chr5_4:45670990-45671009 None:intergenic 50.0%
! TTTGGCCTGTTAGCTTACGG+TGG + chr5_4:45671345-45671364 Msa0853710:intron 50.0%
GCTTTCCACCGTAAGCTAAC+AGG - chr5_4:45671353-45671372 None:intergenic 50.0%
!! AGTTGGTTCTGAACCAGTCG+AGG + chr5_4:45671477-45671496 Msa0853710:CDS 50.0%
AACTGTTCATGAGCCTCGAC+TGG - chr5_4:45671493-45671512 None:intergenic 50.0%
CGAGGCTCATGAACAGTTCA+TGG + chr5_4:45671495-45671514 Msa0853710:CDS 50.0%
! CGCGACGATGATGTTTTGCT+TGG + chr5_4:45671550-45671569 Msa0853710:CDS 50.0%
!! GTGAGGTGTCGAATACCCTC+CGG - chr5_4:45668913-45668932 None:intergenic 55.0%
ATGCAAAGAGGGAACAGGGG+AGG + chr5_4:45668981-45669000 Msa0853710:five_prime_UTR 55.0%
AAGAGGGAACAGGGGAGGAT+TGG + chr5_4:45668986-45669005 Msa0853710:five_prime_UTR 55.0%
AGAGGGAACAGGGGAGGATT+GGG + chr5_4:45668987-45669006 Msa0853710:five_prime_UTR 55.0%
TGGATCTCTGCCTGGGGAAT+GGG - chr5_4:45669177-45669196 None:intergenic 55.0%
GTACTCTGGATCTCTGCCTG+GGG - chr5_4:45669183-45669202 None:intergenic 55.0%
ATGGAACAGCAGCGGCATGT+TGG + chr5_4:45671514-45671533 Msa0853710:CDS 55.0%
CTGGATCTCTGCCTGGGGAA+TGG - chr5_4:45669178-45669197 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 45668899 45671582 45668899 ID=Msa0853710;Name=Msa0853710
chr5_4 mRNA 45668899 45671582 45668899 ID=Msa0853710-mRNA-1;Parent=Msa0853710;Name=Msa0853710-mRNA-1;_AED=0.42;_eAED=0.47;_QI=440|0.66|0.71|0.85|0.33|0.28|7|0|82
chr5_4 exon 45668899 45669046 45668899 ID=Msa0853710-mRNA-1:exon:14392;Parent=Msa0853710-mRNA-1
chr5_4 exon 45669292 45669334 45669292 ID=Msa0853710-mRNA-1:exon:14393;Parent=Msa0853710-mRNA-1
chr5_4 exon 45669411 45669488 45669411 ID=Msa0853710-mRNA-1:exon:14394;Parent=Msa0853710-mRNA-1
chr5_4 exon 45669750 45669854 45669750 ID=Msa0853710-mRNA-1:exon:14395;Parent=Msa0853710-mRNA-1
chr5_4 exon 45669951 45670078 45669951 ID=Msa0853710-mRNA-1:exon:14396;Parent=Msa0853710-mRNA-1
chr5_4 exon 45670710 45670756 45670710 ID=Msa0853710-mRNA-1:exon:14397;Parent=Msa0853710-mRNA-1
chr5_4 exon 45671443 45671582 45671443 ID=Msa0853710-mRNA-1:exon:14398;Parent=Msa0853710-mRNA-1
chr5_4 five_prime_UTR 45668899 45669046 45668899 ID=Msa0853710-mRNA-1:five_prime_utr;Parent=Msa0853710-mRNA-1
chr5_4 five_prime_UTR 45669292 45669334 45669292 ID=Msa0853710-mRNA-1:five_prime_utr;Parent=Msa0853710-mRNA-1
chr5_4 five_prime_UTR 45669411 45669488 45669411 ID=Msa0853710-mRNA-1:five_prime_utr;Parent=Msa0853710-mRNA-1
chr5_4 five_prime_UTR 45669750 45669854 45669750 ID=Msa0853710-mRNA-1:five_prime_utr;Parent=Msa0853710-mRNA-1
chr5_4 five_prime_UTR 45669951 45670016 45669951 ID=Msa0853710-mRNA-1:five_prime_utr;Parent=Msa0853710-mRNA-1
chr5_4 CDS 45670017 45670078 45670017 ID=Msa0853710-mRNA-1:cds;Parent=Msa0853710-mRNA-1
chr5_4 CDS 45670710 45670756 45670710 ID=Msa0853710-mRNA-1:cds;Parent=Msa0853710-mRNA-1
chr5_4 CDS 45671443 45671582 45671443 ID=Msa0853710-mRNA-1:cds;Parent=Msa0853710-mRNA-1
Gene Sequence

>Msa0853710

ATGACAAAGAACTGTATGATAAAAGCCAAGCTTCCGGATATTCACTTCAAGTACATTGAAAGGTTGATAGGTAATGAACAGTTAGATGGTTTTGAATATTTTGTGCAAGTGTTTTTTCACGAGGAGAATATTGATACAAACTCAGTTGGTTCTGAACCAGTCGAGGCTCATGAACAGTTCATGGAACAGCAGCGGCATGTTGGTAATGACGAGAATCGCGACGATGATGTTTTGCTTGGTCAAAATTGA
Protein sequence

>Msa0853710

MTKNCMIKAKLPDIHFKYIERLIGNEQLDGFEYFVQVFFHEENIDTNSVGSEPVEAHEQFMEQQRHVGNDENRDDDVLLGQN*