AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0870320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0870320 MtrunA17_Chr6g0451831 96.842 95 3 0 1 95 1 95 1.99e-65 191
Msa0870320 MtrunA17_Chr2g0334081 43.103 58 31 1 10 65 1 58 1.82e-13 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0870320 AT5G43150.1 43.750 64 36 0 4 67 1 64 8.43e-13 58.9

Find 47 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAAACATCTACAATTTA+AGG 0.057799 6_1:+4710125 Msa0870320:three_prime_UTR
TATTTCTCTTCACAGAAATT+AGG 0.147148 6_1:+4709640 Msa0870320:three_prime_UTR
AAGACTTCACATCATTTCTT+AGG 0.245992 6_1:-4709441 None:intergenic
CGAGACTGTATTGAAGAATA+TGG 0.303346 6_1:+4708357 Msa0870320:exon
AAATTACCAAAATTCCAAAA+TGG 0.317249 6_1:+4709952 Msa0870320:three_prime_UTR
ATTTCTCTTCACAGAAATTA+GGG 0.322081 6_1:+4709641 Msa0870320:three_prime_UTR
ATTACTTGAAATTGTTCAAA+TGG 0.339768 6_1:+4709566 Msa0870320:CDS
AATGAATCATTATCAGAGTT+TGG 0.349611 6_1:+4709508 Msa0870320:CDS
GTGATCCGGGGAGAAAATTT+CGG 0.381711 6_1:+4710058 Msa0870320:three_prime_UTR
CAGCTCAATTGTTTCGTAGT+TGG 0.393155 6_1:+4709735 Msa0870320:three_prime_UTR
TTCTCTTTATTCATGATTGA+AGG 0.411458 6_1:-4709671 None:intergenic
ATAGACTCACACACATGATT+AGG 0.412062 6_1:-4709620 None:intergenic
CATTCTATCAAGTACATAGT+AGG 0.414534 6_1:-4710331 None:intergenic
TCAATCATGAATAAAGAGAA+TGG 0.441154 6_1:+4709674 Msa0870320:three_prime_UTR
CAATCATGAATAAAGAGAAT+GGG 0.445925 6_1:+4709675 Msa0870320:three_prime_UTR
TTACTTGAAATTGTTCAAAT+GGG 0.455771 6_1:+4709567 Msa0870320:CDS
ACAATTTAGTTCGTGCGAAA+AGG 0.463261 6_1:-4710018 None:intergenic
AAAATCTGAGACACAATTCA+TGG 0.464808 6_1:-4710249 None:intergenic
ACTCAAAGACTAATGTGATC+CGG 0.466511 6_1:+4710044 Msa0870320:three_prime_UTR
GTTGTAAGAATCAAGAACTT+TGG 0.472962 6_1:+4709923 Msa0870320:three_prime_UTR
CTCAAAGACTAATGTGATCC+GGG 0.475469 6_1:+4710045 Msa0870320:three_prime_UTR
GAAGATGAAATTCTCTCGGC+AGG 0.497923 6_1:+4708381 Msa0870320:CDS
GAAATCCAAAACCTTCGAAA+AGG 0.500736 6_1:-4708430 None:intergenic
AATTCTAAAACGTGTGGCAA+TGG 0.520815 6_1:-4708452 None:intergenic
TTCGTGCGAAAAGGTTCTAT+AGG 0.523082 6_1:-4710009 None:intergenic
TAAGGGTGGCTACATCAAAT+CGG 0.544196 6_1:-4709855 None:intergenic
AATCATCAATTTAGTCCTTG+AGG 0.549847 6_1:+4709901 Msa0870320:three_prime_UTR
TCTTGATTCTTACAACCTCA+AGG 0.555331 6_1:-4709916 None:intergenic
TCATTATCAGAGTTTGGTGG+TGG 0.560367 6_1:+4709514 Msa0870320:CDS
TGAAATTGTTCAAATGGGCA+AGG 0.567577 6_1:+4709572 Msa0870320:CDS
ATGAAGATATTCGTGTGATG+TGG 0.570449 6_1:+4709470 Msa0870320:CDS
CATATTCTTCAATACAGTCT+CGG 0.572911 6_1:-4708356 None:intergenic
TTTACGAATTCTAAAACGTG+TGG 0.573490 6_1:-4708458 None:intergenic
TGGAGAAGATGAAATTCTCT+CGG 0.589361 6_1:+4708377 Msa0870320:CDS
TCTACAAAAGCAATACATGA+AGG 0.590440 6_1:-4710225 None:intergenic
TCTCTCGGCAGGTGGAGTTG+TGG 0.592580 6_1:+4708392 Msa0870320:CDS
AATCATGAATAAAGAGAATG+GGG 0.601705 6_1:+4709676 Msa0870320:three_prime_UTR
TGAAGATATTCGTGTGATGT+GGG 0.602380 6_1:+4709471 Msa0870320:CDS
GATGAAATTCTCTCGGCAGG+TGG 0.607611 6_1:+4708384 Msa0870320:CDS
ATGGGGTTTCATGTTGAACA+TGG 0.629590 6_1:+4709693 Msa0870320:three_prime_UTR
ATACAAAACCAAGAGATACA+AGG 0.637518 6_1:-4710273 None:intergenic
TTCTTCAATACAGTCTCGGT+AGG 0.640844 6_1:-4708352 None:intergenic
GAATCATTATCAGAGTTTGG+TGG 0.650506 6_1:+4709511 Msa0870320:CDS
TCATCAGTAGGAATCAAACT+CGG 0.659777 6_1:-4709794 None:intergenic
GGGTGGCTACATCAAATCGG+TGG 0.663595 6_1:-4709852 None:intergenic
ATAAGCATTGTGAAGTGTAG+TGG 0.668618 6_1:+4708313 Msa0870320:five_prime_UTR
TCAAAGACTAATGTGATCCG+GGG 0.689237 6_1:+4710046 Msa0870320:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTACTATTAATTGTTG+TGG + chr6_1:4708274-4708293 Msa0870320:five_prime_UTR 15.0%
!!! TTGAAATAAACTTGTTAATT+TGG + chr6_1:4708825-4708844 Msa0870320:intron 15.0%
!!! TGAAATAAACTTGTTAATTT+GGG + chr6_1:4708826-4708845 Msa0870320:intron 15.0%
!!! TTCACATTGAATATTTTTTT+AGG + chr6_1:4709009-4709028 Msa0870320:intron 15.0%
!!! TTTTATTTCAAAAACCATTT+TGG - chr6_1:4709969-4709988 None:intergenic 15.0%
!!! TTTTTTACTATTAATTGTTG+TGG + chr6_1:4708274-4708293 Msa0870320:five_prime_UTR 15.0%
!!! TTGAAATAAACTTGTTAATT+TGG + chr6_1:4708825-4708844 Msa0870320:intron 15.0%
!!! TGAAATAAACTTGTTAATTT+GGG + chr6_1:4708826-4708845 Msa0870320:intron 15.0%
!!! TTCACATTGAATATTTTTTT+AGG + chr6_1:4709009-4709028 Msa0870320:intron 15.0%
!!! TTTTATTTCAAAAACCATTT+TGG - chr6_1:4709969-4709988 None:intergenic 15.0%
!!! CTAAGATATTTTTCATTAGA+CGG + chr6_1:4708192-4708211 Msa0870320:five_prime_UTR 20.0%
!!! TAGTTTTATCAAAAGAAAGA+TGG + chr6_1:4708720-4708739 Msa0870320:intron 20.0%
!!! GTTTAATTTTAATACACTGA+CGG + chr6_1:4708897-4708916 Msa0870320:intron 20.0%
!! TATCCACATATAATTAATCA+TGG - chr6_1:4708951-4708970 None:intergenic 20.0%
!!! TCATCAAAATCAAGATTTTA+AGG - chr6_1:4709080-4709099 None:intergenic 20.0%
!!! TTGATTTTGATGATTATTTG+TGG + chr6_1:4709087-4709106 Msa0870320:intron 20.0%
!! TTGGAATAAACATGTTAATT+TGG + chr6_1:4709334-4709353 Msa0870320:intron 20.0%
!! ATTACTTGAAATTGTTCAAA+TGG + chr6_1:4709566-4709585 Msa0870320:CDS 20.0%
!! TTACTTGAAATTGTTCAAAT+GGG + chr6_1:4709567-4709586 Msa0870320:CDS 20.0%
!!! GTTATTTTCATATACGATAA+GGG - chr6_1:4709875-4709894 None:intergenic 20.0%
!!! AGTTATTTTCATATACGATA+AGG - chr6_1:4709876-4709895 None:intergenic 20.0%
!! AAATTACCAAAATTCCAAAA+TGG + chr6_1:4709952-4709971 Msa0870320:three_prime_UTR 20.0%
!!! TTTGAATTTTGTCAATTTCA+TGG + chr6_1:4710095-4710114 Msa0870320:three_prime_UTR 20.0%
!! TTTCAAACATCTACAATTTA+AGG + chr6_1:4710125-4710144 Msa0870320:three_prime_UTR 20.0%
!!! CTAAGATATTTTTCATTAGA+CGG + chr6_1:4708192-4708211 Msa0870320:five_prime_UTR 20.0%
!!! TAGTTTTATCAAAAGAAAGA+TGG + chr6_1:4708720-4708739 Msa0870320:intron 20.0%
!!! GTTTAATTTTAATACACTGA+CGG + chr6_1:4708897-4708916 Msa0870320:intron 20.0%
!! TATCCACATATAATTAATCA+TGG - chr6_1:4708951-4708970 None:intergenic 20.0%
!!! TCATCAAAATCAAGATTTTA+AGG - chr6_1:4709080-4709099 None:intergenic 20.0%
!!! TTGATTTTGATGATTATTTG+TGG + chr6_1:4709087-4709106 Msa0870320:intron 20.0%
!! TTGGAATAAACATGTTAATT+TGG + chr6_1:4709334-4709353 Msa0870320:intron 20.0%
!! ATTACTTGAAATTGTTCAAA+TGG + chr6_1:4709566-4709585 Msa0870320:CDS 20.0%
!! TTACTTGAAATTGTTCAAAT+GGG + chr6_1:4709567-4709586 Msa0870320:CDS 20.0%
!!! GTTATTTTCATATACGATAA+GGG - chr6_1:4709875-4709894 None:intergenic 20.0%
!!! AGTTATTTTCATATACGATA+AGG - chr6_1:4709876-4709895 None:intergenic 20.0%
!! AAATTACCAAAATTCCAAAA+TGG + chr6_1:4709952-4709971 Msa0870320:three_prime_UTR 20.0%
!!! TTTGAATTTTGTCAATTTCA+TGG + chr6_1:4710095-4710114 Msa0870320:three_prime_UTR 20.0%
!! TTTCAAACATCTACAATTTA+AGG + chr6_1:4710125-4710144 Msa0870320:three_prime_UTR 20.0%
! TTTGTTACAAAGTACATGTT+TGG + chr6_1:4708525-4708544 Msa0870320:intron 25.0%
! CACGTTAATTGTAACATATT+TGG + chr6_1:4708594-4708613 Msa0870320:intron 25.0%
! GTGAATTGTTGTAACATATT+TGG + chr6_1:4708652-4708671 Msa0870320:intron 25.0%
!!! CATTGAATATTTTTTTAGGC+CGG + chr6_1:4709013-4709032 Msa0870320:intron 25.0%
! TGAATTGACAATGAATTTAG+CGG - chr6_1:4709267-4709286 None:intergenic 25.0%
! AAACAATGAGTTACTGAAAT+TGG + chr6_1:4709396-4709415 Msa0870320:intron 25.0%
! AATGAATCATTATCAGAGTT+TGG + chr6_1:4709508-4709527 Msa0870320:CDS 25.0%
!! TGAATCTTCTTGATTTTGTT+TGG - chr6_1:4709547-4709566 None:intergenic 25.0%
! TATTTCTCTTCACAGAAATT+AGG + chr6_1:4709640-4709659 Msa0870320:three_prime_UTR 25.0%
! ATTTCTCTTCACAGAAATTA+GGG + chr6_1:4709641-4709660 Msa0870320:three_prime_UTR 25.0%
! TTCTCTTTATTCATGATTGA+AGG - chr6_1:4709674-4709693 None:intergenic 25.0%
! TCAATCATGAATAAAGAGAA+TGG + chr6_1:4709674-4709693 Msa0870320:three_prime_UTR 25.0%
! CAATCATGAATAAAGAGAAT+GGG + chr6_1:4709675-4709694 Msa0870320:three_prime_UTR 25.0%
! AATCATGAATAAAGAGAATG+GGG + chr6_1:4709676-4709695 Msa0870320:three_prime_UTR 25.0%
!!! TTGGAATTTTGGTAATTTCA+AGG - chr6_1:4709950-4709969 None:intergenic 25.0%
!!! CAAAAACCATTTTGGAATTT+TGG - chr6_1:4709961-4709980 None:intergenic 25.0%
!!! GAAAATAATGTGTAGTTTTG+TGG + chr6_1:4710168-4710187 Msa0870320:three_prime_UTR 25.0%
! TTTGTTACAAAGTACATGTT+TGG + chr6_1:4708525-4708544 Msa0870320:intron 25.0%
! CACGTTAATTGTAACATATT+TGG + chr6_1:4708594-4708613 Msa0870320:intron 25.0%
! GTGAATTGTTGTAACATATT+TGG + chr6_1:4708652-4708671 Msa0870320:intron 25.0%
!!! CATTGAATATTTTTTTAGGC+CGG + chr6_1:4709013-4709032 Msa0870320:intron 25.0%
! TGAATTGACAATGAATTTAG+CGG - chr6_1:4709267-4709286 None:intergenic 25.0%
! AAACAATGAGTTACTGAAAT+TGG + chr6_1:4709396-4709415 Msa0870320:intron 25.0%
! AATGAATCATTATCAGAGTT+TGG + chr6_1:4709508-4709527 Msa0870320:CDS 25.0%
!! TGAATCTTCTTGATTTTGTT+TGG - chr6_1:4709547-4709566 None:intergenic 25.0%
! TATTTCTCTTCACAGAAATT+AGG + chr6_1:4709640-4709659 Msa0870320:three_prime_UTR 25.0%
! ATTTCTCTTCACAGAAATTA+GGG + chr6_1:4709641-4709660 Msa0870320:three_prime_UTR 25.0%
! TTCTCTTTATTCATGATTGA+AGG - chr6_1:4709674-4709693 None:intergenic 25.0%
! TCAATCATGAATAAAGAGAA+TGG + chr6_1:4709674-4709693 Msa0870320:three_prime_UTR 25.0%
! CAATCATGAATAAAGAGAAT+GGG + chr6_1:4709675-4709694 Msa0870320:three_prime_UTR 25.0%
! AATCATGAATAAAGAGAATG+GGG + chr6_1:4709676-4709695 Msa0870320:three_prime_UTR 25.0%
!!! TTGGAATTTTGGTAATTTCA+AGG - chr6_1:4709950-4709969 None:intergenic 25.0%
!!! CAAAAACCATTTTGGAATTT+TGG - chr6_1:4709961-4709980 None:intergenic 25.0%
!!! GAAAATAATGTGTAGTTTTG+TGG + chr6_1:4710168-4710187 Msa0870320:three_prime_UTR 25.0%
CATATTCTTCAATACAGTCT+CGG - chr6_1:4708359-4708378 None:intergenic 30.0%
!! TTTACGAATTCTAAAACGTG+TGG - chr6_1:4708461-4708480 None:intergenic 30.0%
!!! CGTTTTAGAATTCGTAAAAC+TGG + chr6_1:4708463-4708482 Msa0870320:CDS 30.0%
AAAAATCAGACAGAACTACA+AGG - chr6_1:4708868-4708887 None:intergenic 30.0%
!! TTCTGTCTGATTTTTGAAGA+TGG + chr6_1:4708873-4708892 Msa0870320:intron 30.0%
! AACAATTTTTACGGCATTAG+AGG + chr6_1:4708924-4708943 Msa0870320:intron 30.0%
GGACCATGATTAATTATATG+TGG + chr6_1:4708945-4708964 Msa0870320:intron 30.0%
ATTTGATTCTTATACACTGC+CGG - chr6_1:4709035-4709054 None:intergenic 30.0%
TGGTGAGTTTGATGAAATTA+TGG + chr6_1:4709175-4709194 Msa0870320:intron 30.0%
ACATAATCAGAGAGCTATAT+AGG - chr6_1:4709373-4709392 None:intergenic 30.0%
TGAAATTGGAAAAATGTGCA+GGG + chr6_1:4709410-4709429 Msa0870320:intron 30.0%
AAGACTTCACATCATTTCTT+AGG - chr6_1:4709444-4709463 None:intergenic 30.0%
! GATTAGGAGTGAGAATTTTA+TGG - chr6_1:4709607-4709626 None:intergenic 30.0%
!!! ATCGAGTTTTTTTCATCAGT+AGG - chr6_1:4709809-4709828 None:intergenic 30.0%
! ATTTTCATATACGATAAGGG+TGG - chr6_1:4709872-4709891 None:intergenic 30.0%
AATCATCAATTTAGTCCTTG+AGG + chr6_1:4709901-4709920 Msa0870320:three_prime_UTR 30.0%
GTTGTAAGAATCAAGAACTT+TGG + chr6_1:4709923-4709942 Msa0870320:three_prime_UTR 30.0%
TCTACAAAAGCAATACATGA+AGG - chr6_1:4710228-4710247 None:intergenic 30.0%
! AAAATCTGAGACACAATTCA+TGG - chr6_1:4710252-4710271 None:intergenic 30.0%
! AGATTTTACCTTGTATCTCT+TGG + chr6_1:4710265-4710284 Msa0870320:three_prime_UTR 30.0%
ATACAAAACCAAGAGATACA+AGG - chr6_1:4710276-4710295 None:intergenic 30.0%
CATTCTATCAAGTACATAGT+AGG - chr6_1:4710334-4710353 None:intergenic 30.0%
CATATTCTTCAATACAGTCT+CGG - chr6_1:4708359-4708378 None:intergenic 30.0%
!! TTTACGAATTCTAAAACGTG+TGG - chr6_1:4708461-4708480 None:intergenic 30.0%
!!! CGTTTTAGAATTCGTAAAAC+TGG + chr6_1:4708463-4708482 Msa0870320:CDS 30.0%
AAAAATCAGACAGAACTACA+AGG - chr6_1:4708868-4708887 None:intergenic 30.0%
!! TTCTGTCTGATTTTTGAAGA+TGG + chr6_1:4708873-4708892 Msa0870320:intron 30.0%
! AACAATTTTTACGGCATTAG+AGG + chr6_1:4708924-4708943 Msa0870320:intron 30.0%
GGACCATGATTAATTATATG+TGG + chr6_1:4708945-4708964 Msa0870320:intron 30.0%
ATTTGATTCTTATACACTGC+CGG - chr6_1:4709035-4709054 None:intergenic 30.0%
TGGTGAGTTTGATGAAATTA+TGG + chr6_1:4709175-4709194 Msa0870320:intron 30.0%
ACATAATCAGAGAGCTATAT+AGG - chr6_1:4709373-4709392 None:intergenic 30.0%
TGAAATTGGAAAAATGTGCA+GGG + chr6_1:4709410-4709429 Msa0870320:intron 30.0%
AAGACTTCACATCATTTCTT+AGG - chr6_1:4709444-4709463 None:intergenic 30.0%
! GATTAGGAGTGAGAATTTTA+TGG - chr6_1:4709607-4709626 None:intergenic 30.0%
!!! ATCGAGTTTTTTTCATCAGT+AGG - chr6_1:4709809-4709828 None:intergenic 30.0%
! ATTTTCATATACGATAAGGG+TGG - chr6_1:4709872-4709891 None:intergenic 30.0%
AATCATCAATTTAGTCCTTG+AGG + chr6_1:4709901-4709920 Msa0870320:three_prime_UTR 30.0%
GTTGTAAGAATCAAGAACTT+TGG + chr6_1:4709923-4709942 Msa0870320:three_prime_UTR 30.0%
TCTACAAAAGCAATACATGA+AGG - chr6_1:4710228-4710247 None:intergenic 30.0%
! AAAATCTGAGACACAATTCA+TGG - chr6_1:4710252-4710271 None:intergenic 30.0%
! AGATTTTACCTTGTATCTCT+TGG + chr6_1:4710265-4710284 Msa0870320:three_prime_UTR 30.0%
ATACAAAACCAAGAGATACA+AGG - chr6_1:4710276-4710295 None:intergenic 30.0%
CATTCTATCAAGTACATAGT+AGG - chr6_1:4710334-4710353 None:intergenic 30.0%
! ATAAGCATTGTGAAGTGTAG+TGG + chr6_1:4708313-4708332 Msa0870320:five_prime_UTR 35.0%
CGAGACTGTATTGAAGAATA+TGG + chr6_1:4708357-4708376 Msa0870320:exon 35.0%
TGGAGAAGATGAAATTCTCT+CGG + chr6_1:4708377-4708396 Msa0870320:CDS 35.0%
GAAATCCAAAACCTTCGAAA+AGG - chr6_1:4708433-4708452 None:intergenic 35.0%
!! AATTCTAAAACGTGTGGCAA+TGG - chr6_1:4708455-4708474 None:intergenic 35.0%
CACGAGTAGTTTGACAAAAT+CGG + chr6_1:4708620-4708639 Msa0870320:intron 35.0%
GAGTTTAGCAGAATCATGAT+CGG - chr6_1:4708746-4708765 None:intergenic 35.0%
! GACGGTGCAAACAATTTTTA+CGG + chr6_1:4708915-4708934 Msa0870320:intron 35.0%
GCTGAACAATAATCTTCAGA+AGG - chr6_1:4709058-4709077 None:intergenic 35.0%
TATAGCTTCTGCTAAAATCG+TGG + chr6_1:4709227-4709246 Msa0870320:intron 35.0%
CAATGAATTTAGCGGAATCA+TGG - chr6_1:4709259-4709278 None:intergenic 35.0%
ACTTGAGTGAATGCAAAGTT+TGG + chr6_1:4709315-4709334 Msa0870320:intron 35.0%
CTGAAATTGGAAAAATGTGC+AGG + chr6_1:4709409-4709428 Msa0870320:intron 35.0%
ATGAAGATATTCGTGTGATG+TGG + chr6_1:4709470-4709489 Msa0870320:CDS 35.0%
TGAAGATATTCGTGTGATGT+GGG + chr6_1:4709471-4709490 Msa0870320:CDS 35.0%
GAATCATTATCAGAGTTTGG+TGG + chr6_1:4709511-4709530 Msa0870320:CDS 35.0%
TGAAATTGTTCAAATGGGCA+AGG + chr6_1:4709572-4709591 Msa0870320:CDS 35.0%
ATAGACTCACACACATGATT+AGG - chr6_1:4709623-4709642 None:intergenic 35.0%
TCATCAGTAGGAATCAAACT+CGG - chr6_1:4709797-4709816 None:intergenic 35.0%
TCTTGATTCTTACAACCTCA+AGG - chr6_1:4709919-4709938 None:intergenic 35.0%
! ACAATTTAGTTCGTGCGAAA+AGG - chr6_1:4710021-4710040 None:intergenic 35.0%
ACTCAAAGACTAATGTGATC+CGG + chr6_1:4710044-4710063 Msa0870320:three_prime_UTR 35.0%
! ATAAGCATTGTGAAGTGTAG+TGG + chr6_1:4708313-4708332 Msa0870320:five_prime_UTR 35.0%
CGAGACTGTATTGAAGAATA+TGG + chr6_1:4708357-4708376 Msa0870320:exon 35.0%
TGGAGAAGATGAAATTCTCT+CGG + chr6_1:4708377-4708396 Msa0870320:CDS 35.0%
GAAATCCAAAACCTTCGAAA+AGG - chr6_1:4708433-4708452 None:intergenic 35.0%
!! AATTCTAAAACGTGTGGCAA+TGG - chr6_1:4708455-4708474 None:intergenic 35.0%
CACGAGTAGTTTGACAAAAT+CGG + chr6_1:4708620-4708639 Msa0870320:intron 35.0%
GAGTTTAGCAGAATCATGAT+CGG - chr6_1:4708746-4708765 None:intergenic 35.0%
! GACGGTGCAAACAATTTTTA+CGG + chr6_1:4708915-4708934 Msa0870320:intron 35.0%
GCTGAACAATAATCTTCAGA+AGG - chr6_1:4709058-4709077 None:intergenic 35.0%
TATAGCTTCTGCTAAAATCG+TGG + chr6_1:4709227-4709246 Msa0870320:intron 35.0%
CAATGAATTTAGCGGAATCA+TGG - chr6_1:4709259-4709278 None:intergenic 35.0%
ACTTGAGTGAATGCAAAGTT+TGG + chr6_1:4709315-4709334 Msa0870320:intron 35.0%
CTGAAATTGGAAAAATGTGC+AGG + chr6_1:4709409-4709428 Msa0870320:intron 35.0%
ATGAAGATATTCGTGTGATG+TGG + chr6_1:4709470-4709489 Msa0870320:CDS 35.0%
TGAAGATATTCGTGTGATGT+GGG + chr6_1:4709471-4709490 Msa0870320:CDS 35.0%
GAATCATTATCAGAGTTTGG+TGG + chr6_1:4709511-4709530 Msa0870320:CDS 35.0%
TGAAATTGTTCAAATGGGCA+AGG + chr6_1:4709572-4709591 Msa0870320:CDS 35.0%
ATAGACTCACACACATGATT+AGG - chr6_1:4709623-4709642 None:intergenic 35.0%
TCATCAGTAGGAATCAAACT+CGG - chr6_1:4709797-4709816 None:intergenic 35.0%
TCTTGATTCTTACAACCTCA+AGG - chr6_1:4709919-4709938 None:intergenic 35.0%
! ACAATTTAGTTCGTGCGAAA+AGG - chr6_1:4710021-4710040 None:intergenic 35.0%
ACTCAAAGACTAATGTGATC+CGG + chr6_1:4710044-4710063 Msa0870320:three_prime_UTR 35.0%
TTCTTCAATACAGTCTCGGT+AGG - chr6_1:4708355-4708374 None:intergenic 40.0%
! ACATCATGCATCCTTTTCGA+AGG + chr6_1:4708419-4708438 Msa0870320:CDS 40.0%
! TGCATCCTTTTCGAAGGTTT+TGG + chr6_1:4708425-4708444 Msa0870320:CDS 40.0%
TTGTAGCGTGTTTGAATTGC+TGG + chr6_1:4709155-4709174 Msa0870320:intron 40.0%
! TCATTATCAGAGTTTGGTGG+TGG + chr6_1:4709514-4709533 Msa0870320:CDS 40.0%
ATGGGGTTTCATGTTGAACA+TGG + chr6_1:4709693-4709712 Msa0870320:three_prime_UTR 40.0%
CAGCTCAATTGTTTCGTAGT+TGG + chr6_1:4709735-4709754 Msa0870320:three_prime_UTR 40.0%
TAAGGGTGGCTACATCAAAT+CGG - chr6_1:4709858-4709877 None:intergenic 40.0%
TTCGTGCGAAAAGGTTCTAT+AGG - chr6_1:4710012-4710031 None:intergenic 40.0%
CTCAAAGACTAATGTGATCC+GGG + chr6_1:4710045-4710064 Msa0870320:three_prime_UTR 40.0%
TCAAAGACTAATGTGATCCG+GGG + chr6_1:4710046-4710065 Msa0870320:three_prime_UTR 40.0%
! TGTAACCGAAATTTTCTCCC+CGG - chr6_1:4710066-4710085 None:intergenic 40.0%
TTCTTCAATACAGTCTCGGT+AGG - chr6_1:4708355-4708374 None:intergenic 40.0%
! ACATCATGCATCCTTTTCGA+AGG + chr6_1:4708419-4708438 Msa0870320:CDS 40.0%
! TGCATCCTTTTCGAAGGTTT+TGG + chr6_1:4708425-4708444 Msa0870320:CDS 40.0%
TTGTAGCGTGTTTGAATTGC+TGG + chr6_1:4709155-4709174 Msa0870320:intron 40.0%
! TCATTATCAGAGTTTGGTGG+TGG + chr6_1:4709514-4709533 Msa0870320:CDS 40.0%
ATGGGGTTTCATGTTGAACA+TGG + chr6_1:4709693-4709712 Msa0870320:three_prime_UTR 40.0%
CAGCTCAATTGTTTCGTAGT+TGG + chr6_1:4709735-4709754 Msa0870320:three_prime_UTR 40.0%
TAAGGGTGGCTACATCAAAT+CGG - chr6_1:4709858-4709877 None:intergenic 40.0%
TTCGTGCGAAAAGGTTCTAT+AGG - chr6_1:4710012-4710031 None:intergenic 40.0%
CTCAAAGACTAATGTGATCC+GGG + chr6_1:4710045-4710064 Msa0870320:three_prime_UTR 40.0%
TCAAAGACTAATGTGATCCG+GGG + chr6_1:4710046-4710065 Msa0870320:three_prime_UTR 40.0%
! TGTAACCGAAATTTTCTCCC+CGG - chr6_1:4710066-4710085 None:intergenic 40.0%
GAAGATGAAATTCTCTCGGC+AGG + chr6_1:4708381-4708400 Msa0870320:CDS 45.0%
GTGATCCGGGGAGAAAATTT+CGG + chr6_1:4710058-4710077 Msa0870320:three_prime_UTR 45.0%
GAAGATGAAATTCTCTCGGC+AGG + chr6_1:4708381-4708400 Msa0870320:CDS 45.0%
GTGATCCGGGGAGAAAATTT+CGG + chr6_1:4710058-4710077 Msa0870320:three_prime_UTR 45.0%
GATGAAATTCTCTCGGCAGG+TGG + chr6_1:4708384-4708403 Msa0870320:CDS 50.0%
GATGAAATTCTCTCGGCAGG+TGG + chr6_1:4708384-4708403 Msa0870320:CDS 50.0%
GGGTGGCTACATCAAATCGG+TGG - chr6_1:4709855-4709874 None:intergenic 55.0%
GGGTGGCTACATCAAATCGG+TGG - chr6_1:4709855-4709874 None:intergenic 55.0%
TCTCTCGGCAGGTGGAGTTG+TGG + chr6_1:4708392-4708411 Msa0870320:CDS 60.0%
TCTCTCGGCAGGTGGAGTTG+TGG + chr6_1:4708392-4708411 Msa0870320:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 4708145 4710380 4708145 ID=Msa0870320;Name=Msa0870320
chr6_1 mRNA 4708145 4710380 4708145 ID=Msa0870320-mRNA-1;Parent=Msa0870320;Name=Msa0870320-mRNA-1;_AED=0.00;_eAED=0.00;_QI=231|1|1|1|1|1|2|771|95
chr6_1 exon 4708145 4708484 4708145 ID=Msa0870320-mRNA-1:exon:1474;Parent=Msa0870320-mRNA-1
chr6_1 exon 4709431 4710380 4709431 ID=Msa0870320-mRNA-1:exon:1475;Parent=Msa0870320-mRNA-1
chr6_1 five_prime_UTR 4708145 4708375 4708145 ID=Msa0870320-mRNA-1:five_prime_utr;Parent=Msa0870320-mRNA-1
chr6_1 CDS 4708376 4708484 4708376 ID=Msa0870320-mRNA-1:cds;Parent=Msa0870320-mRNA-1
chr6_1 CDS 4709431 4709609 4709431 ID=Msa0870320-mRNA-1:cds;Parent=Msa0870320-mRNA-1
chr6_1 three_prime_UTR 4709610 4710380 4709610 ID=Msa0870320-mRNA-1:three_prime_utr;Parent=Msa0870320-mRNA-1
Gene Sequence

>Msa0870320

ATGGAGAAGATGAAATTCTCTCGGCAGGTGGAGTTGTGGCAGAACATCATGCATCCTTTTCGAAGGTTTTGGATTTCCATTGCCACACGTTTTAGAATTCGTAAAACTGGGTTGAAGAACCTAAGAAATGATGTGAAGTCTTGTGAATATGAAGATATTCGTGTGATGTGGGAGATGCTAAACAGAAATGAATCATTATCAGAGTTTGGTGGTGGTAATTCTCCAAACAAAATCAAGAAGATTCATTACTTGAAATTGTTCAAATGGGCAAGGTGTGCTGCTCCATAA
Protein sequence

>Msa0870320

MEKMKFSRQVELWQNIMHPFRRFWISIATRFRIRKTGLKNLRNDVKSCEYEDIRVMWEMLNRNESLSEFGGGNSPNKIKKIHYLKLFKWARCAAP*