AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0936030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0936030 MtrunA17_Chr6g0451831 97.895 95 2 0 1 95 1 95 1.13e-66 195
Msa0936030 MtrunA17_Chr2g0334081 43.103 58 31 1 10 65 1 58 1.42e-13 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0936030 AT5G43150.1 45.312 64 35 0 4 67 1 64 4.16e-14 62.4

Find 48 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAAACATCTACAATTTA+AGG 0.057799 6_3:+6636879 Msa0936030:three_prime_UTR
TATTTCTCTTCACAGAAATT+AGG 0.147148 6_3:+6636394 Msa0936030:three_prime_UTR
CTATGTACTGTCGGTGTAAA+AGG 0.209307 6_3:+6637090 Msa0936030:three_prime_UTR
AAGACTTCACATCATTTCTT+AGG 0.245992 6_3:-6636192 None:intergenic
ATTTCCATTGCCACACGTTT+TGG 0.291787 6_3:+6635081 Msa0936030:CDS
GAAATCCAAAACCTTCTAAA+AGG 0.302858 6_3:-6635063 None:intergenic
CGAGACTGTATTGAAGAATA+TGG 0.303346 6_3:+6634990 Msa0936030:exon
AAATTAACAAAATTCCAAAA+TGG 0.316346 6_3:+6636706 Msa0936030:three_prime_UTR
ATTTCTCTTCACAGAAATTA+GGG 0.322081 6_3:+6636395 Msa0936030:three_prime_UTR
AATGAATCATTATCAGAGTT+TGG 0.349611 6_3:+6636259 Msa0936030:CDS
AATCATGAATAAAGAGAATA+GGG 0.352912 6_3:+6636430 Msa0936030:three_prime_UTR
ATTACTTGAAATTATTCAAA+TGG 0.354776 6_3:+6636317 Msa0936030:CDS
CAGCTCAATTGTTTCATAGT+TGG 0.366825 6_3:+6636489 Msa0936030:three_prime_UTR
GTGATCCGGGGAGAAAATTT+CGG 0.381711 6_3:+6636812 Msa0936030:three_prime_UTR
CAATCATGAATAAAGAGAAT+AGG 0.388432 6_3:+6636429 Msa0936030:three_prime_UTR
TAAGGGTGGCTACATCAAAT+TGG 0.395071 6_3:-6636609 None:intergenic
TTACTTGAAATTATTCAAAT+GGG 0.457800 6_3:+6636318 Msa0936030:CDS
ACAATTTAGTTCGTGCGAAA+AGG 0.463261 6_3:-6636772 None:intergenic
AAAATCTGAGACACAATTCA+TGG 0.464808 6_3:-6637003 None:intergenic
ACTCAAAGACTAATGTGATC+CGG 0.466511 6_3:+6636798 Msa0936030:three_prime_UTR
GTTGTAAGAATCAAGAACTT+TGG 0.472962 6_3:+6636677 Msa0936030:three_prime_UTR
CTCAAAGACTAATGTGATCC+GGG 0.475469 6_3:+6636799 Msa0936030:three_prime_UTR
TTACACCGACAGTACATAGT+AGG 0.477017 6_3:-6637086 None:intergenic
GAAGATGAAATTCTCTCGGC+AGG 0.497923 6_3:+6635014 Msa0936030:CDS
GACTCACACACATGATTAGT+AGG 0.504425 6_3:-6636371 None:intergenic
TTCGTGCGAAAAGGTTCTAT+AGG 0.523082 6_3:-6636763 None:intergenic
TGAAATTATTCAAATGGGCA+AGG 0.527275 6_3:+6636323 Msa0936030:CDS
AATTCCAAAACGTGTGGCAA+TGG 0.529168 6_3:-6635085 None:intergenic
AATCATCAATTTAGTCCTTG+AGG 0.549847 6_3:+6636655 Msa0936030:three_prime_UTR
TCTTGATTCTTACAACCTCA+AGG 0.555331 6_3:-6636670 None:intergenic
TCATTATCAGAGTTTGGTGG+TGG 0.560367 6_3:+6636265 Msa0936030:CDS
ATGAAGATATTCGTGTGATG+TGG 0.570449 6_3:+6636221 Msa0936030:CDS
CATATTCTTCAATACAGTCT+CGG 0.572911 6_3:-6634989 None:intergenic
GGGTGGCTACATCAAATTGG+TGG 0.578105 6_3:-6636606 None:intergenic
TGGAGAAGATGAAATTCTCT+CGG 0.589361 6_3:+6635010 Msa0936030:CDS
TCTACAAAAGCAATACATGA+AGG 0.590440 6_3:-6636979 None:intergenic
ACATAAACATTGTGAAGTAG+TGG 0.592269 6_3:+6634946 Msa0936030:five_prime_UTR
TCTCTCGGCAGGTGGAGTTG+TGG 0.592580 6_3:+6635025 Msa0936030:CDS
TGAAGATATTCGTGTGATGT+GGG 0.602380 6_3:+6636222 Msa0936030:CDS
GATGAAATTCTCTCGGCAGG+TGG 0.607611 6_3:+6635017 Msa0936030:CDS
TTTACGAATTCCAAAACGTG+TGG 0.611706 6_3:-6635091 None:intergenic
ATACAAAACCAAGAGATACA+AGG 0.637518 6_3:-6637027 None:intergenic
ATAGGGTTTCATGTTGAACA+TGG 0.640427 6_3:+6636447 Msa0936030:three_prime_UTR
TTCTTCAATACAGTCTCGGT+AGG 0.640844 6_3:-6634985 None:intergenic
GAATCATTATCAGAGTTTGG+TGG 0.650506 6_3:+6636262 Msa0936030:CDS
TCTTACCTACTATGTACTGT+CGG 0.656678 6_3:+6637081 Msa0936030:three_prime_UTR
TCATCAGTAGGAATCAAACT+CGG 0.659777 6_3:-6636548 None:intergenic
TCAAAGACTAATGTGATCCG+GGG 0.689237 6_3:+6636800 Msa0936030:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAATAATATAGACT+TGG - chr6_3:6634858-6634877 None:intergenic 10.0%
!!! TAAAAAAGAAAAAGATTTTA+AGG - chr6_3:6635708-6635727 None:intergenic 10.0%
!! AAAAAATAATAATATAGACT+TGG - chr6_3:6634858-6634877 None:intergenic 10.0%
!!! TAAAAAAGAAAAAGATTTTA+AGG - chr6_3:6635708-6635727 None:intergenic 10.0%
!!! TTTTTTACTATTAATTGTTG+TGG + chr6_3:6634897-6634916 Msa0936030:five_prime_UTR 15.0%
!! ATTACTTGAAATTATTCAAA+TGG + chr6_3:6636317-6636336 Msa0936030:CDS 15.0%
!! TTACTTGAAATTATTCAAAT+GGG + chr6_3:6636318-6636337 Msa0936030:CDS 15.0%
!! AAATTAACAAAATTCCAAAA+TGG + chr6_3:6636706-6636725 Msa0936030:three_prime_UTR 15.0%
!!! TTTTTTACTATTAATTGTTG+TGG + chr6_3:6634897-6634916 Msa0936030:five_prime_UTR 15.0%
!! ATTACTTGAAATTATTCAAA+TGG + chr6_3:6636317-6636336 Msa0936030:CDS 15.0%
!! TTACTTGAAATTATTCAAAT+GGG + chr6_3:6636318-6636337 Msa0936030:CDS 15.0%
!! AAATTAACAAAATTCCAAAA+TGG + chr6_3:6636706-6636725 Msa0936030:three_prime_UTR 15.0%
!!! CTTAGATATTTTTCATTAGA+CGG + chr6_3:6634820-6634839 Msa0936030:five_prime_UTR 20.0%
!! GTAATAATTCAAAACATGTT+TGG + chr6_3:6635118-6635137 Msa0936030:intron 20.0%
!! GTGAATTATTGTAACATATT+TGG + chr6_3:6635286-6635305 Msa0936030:intron 20.0%
!!! TTTTATCAAAATAGTGATTC+TGG + chr6_3:6635351-6635370 Msa0936030:intron 20.0%
!! TTGAAATAACCTTGTTAATT+TGG + chr6_3:6635440-6635459 Msa0936030:intron 20.0%
!! ATCAAATCACTCAAAATATT+TGG - chr6_3:6635603-6635622 None:intergenic 20.0%
!!! TCACATTGAATATTCTTTTT+AGG + chr6_3:6635636-6635655 Msa0936030:intron 20.0%
!!! AATCTTTTTCTTTTTTATGC+CGG + chr6_3:6635711-6635730 Msa0936030:intron 20.0%
!!! TCTTCAAAATCAAGATTTTA+AGG - chr6_3:6635779-6635798 None:intergenic 20.0%
!!! TCATCAAAATCAAGATTTTA+AGG - chr6_3:6635831-6635850 None:intergenic 20.0%
!!! TTGATTTTGATGATTATTTG+TGG + chr6_3:6635838-6635857 Msa0936030:intron 20.0%
!! TTGGAATAAACATGTTAATT+TGG + chr6_3:6636085-6636104 Msa0936030:intron 20.0%
!!! TGAATTTTCTTGATTTTGTT+TGG - chr6_3:6636298-6636317 None:intergenic 20.0%
!! AATCATGAATAAAGAGAATA+GGG + chr6_3:6636430-6636449 Msa0936030:three_prime_UTR 20.0%
!!! GTTATTTTCATATACGATAA+GGG - chr6_3:6636629-6636648 None:intergenic 20.0%
!!! AGTTATTTTCATATACGATA+AGG - chr6_3:6636630-6636649 None:intergenic 20.0%
!!! TTGGAATTTTGTTAATTTCA+AGG - chr6_3:6636704-6636723 None:intergenic 20.0%
!!! TTTTATTTCAGAAACCATTT+TGG - chr6_3:6636723-6636742 None:intergenic 20.0%
!!! TTTTGAATTTTGTCAATTTC+AGG + chr6_3:6636848-6636867 Msa0936030:three_prime_UTR 20.0%
!!! TTTGAATTTTGTCAATTTCA+GGG + chr6_3:6636849-6636868 Msa0936030:three_prime_UTR 20.0%
!! TTTCAAACATCTACAATTTA+AGG + chr6_3:6636879-6636898 Msa0936030:three_prime_UTR 20.0%
!!! CTTAGATATTTTTCATTAGA+CGG + chr6_3:6634820-6634839 Msa0936030:five_prime_UTR 20.0%
!! GTAATAATTCAAAACATGTT+TGG + chr6_3:6635118-6635137 Msa0936030:intron 20.0%
!! GTGAATTATTGTAACATATT+TGG + chr6_3:6635286-6635305 Msa0936030:intron 20.0%
!!! TTTTATCAAAATAGTGATTC+TGG + chr6_3:6635351-6635370 Msa0936030:intron 20.0%
!! TTGAAATAACCTTGTTAATT+TGG + chr6_3:6635440-6635459 Msa0936030:intron 20.0%
!! ATCAAATCACTCAAAATATT+TGG - chr6_3:6635603-6635622 None:intergenic 20.0%
!!! TCACATTGAATATTCTTTTT+AGG + chr6_3:6635636-6635655 Msa0936030:intron 20.0%
!!! AATCTTTTTCTTTTTTATGC+CGG + chr6_3:6635711-6635730 Msa0936030:intron 20.0%
!!! TCTTCAAAATCAAGATTTTA+AGG - chr6_3:6635779-6635798 None:intergenic 20.0%
!!! TCATCAAAATCAAGATTTTA+AGG - chr6_3:6635831-6635850 None:intergenic 20.0%
!!! TTGATTTTGATGATTATTTG+TGG + chr6_3:6635838-6635857 Msa0936030:intron 20.0%
!! TTGGAATAAACATGTTAATT+TGG + chr6_3:6636085-6636104 Msa0936030:intron 20.0%
!!! TGAATTTTCTTGATTTTGTT+TGG - chr6_3:6636298-6636317 None:intergenic 20.0%
!! AATCATGAATAAAGAGAATA+GGG + chr6_3:6636430-6636449 Msa0936030:three_prime_UTR 20.0%
!!! GTTATTTTCATATACGATAA+GGG - chr6_3:6636629-6636648 None:intergenic 20.0%
!!! AGTTATTTTCATATACGATA+AGG - chr6_3:6636630-6636649 None:intergenic 20.0%
!!! TTGGAATTTTGTTAATTTCA+AGG - chr6_3:6636704-6636723 None:intergenic 20.0%
!!! TTTTATTTCAGAAACCATTT+TGG - chr6_3:6636723-6636742 None:intergenic 20.0%
!!! TTTTGAATTTTGTCAATTTC+AGG + chr6_3:6636848-6636867 Msa0936030:three_prime_UTR 20.0%
!!! TTTGAATTTTGTCAATTTCA+GGG + chr6_3:6636849-6636868 Msa0936030:three_prime_UTR 20.0%
!! TTTCAAACATCTACAATTTA+AGG + chr6_3:6636879-6636898 Msa0936030:three_prime_UTR 20.0%
!!! GGTCTTTTTTCTGTATTTTT+TGG + chr6_3:6635139-6635158 Msa0936030:intron 25.0%
! TTGGTTATAAAGTACATGTT+TGG + chr6_3:6635158-6635177 Msa0936030:intron 25.0%
! ATTTCTTGTCCAAATTAACA+AGG - chr6_3:6635452-6635471 None:intergenic 25.0%
! TCTTCAAAAATCAGATAGAA+CGG - chr6_3:6635479-6635498 None:intergenic 25.0%
!!! TTCTATCTGATTTTTGAAGA+TGG + chr6_3:6635479-6635498 Msa0936030:intron 25.0%
!!! ATTGAATATTCTTTTTAGGC+CGG + chr6_3:6635640-6635659 Msa0936030:intron 25.0%
! AAGGATTTGATTCTTATACA+CGG - chr6_3:6635737-6635756 None:intergenic 25.0%
! CTGAATCAATAATCTTCTAA+AGG - chr6_3:6635756-6635775 None:intergenic 25.0%
! TGAATTGACAATGAATTTAG+CGG - chr6_3:6636018-6636037 None:intergenic 25.0%
! AAACAATGAGTTACTGAAAT+TGG + chr6_3:6636147-6636166 Msa0936030:intron 25.0%
! AATGAATCATTATCAGAGTT+TGG + chr6_3:6636259-6636278 Msa0936030:CDS 25.0%
! TATTTCTCTTCACAGAAATT+AGG + chr6_3:6636394-6636413 Msa0936030:three_prime_UTR 25.0%
! ATTTCTCTTCACAGAAATTA+GGG + chr6_3:6636395-6636414 Msa0936030:three_prime_UTR 25.0%
! CAATCATGAATAAAGAGAAT+AGG + chr6_3:6636429-6636448 Msa0936030:three_prime_UTR 25.0%
!!! GAAAATAATGTGTAGTTTTG+TGG + chr6_3:6636922-6636941 Msa0936030:three_prime_UTR 25.0%
!!! GGTCTTTTTTCTGTATTTTT+TGG + chr6_3:6635139-6635158 Msa0936030:intron 25.0%
! TTGGTTATAAAGTACATGTT+TGG + chr6_3:6635158-6635177 Msa0936030:intron 25.0%
! ATTTCTTGTCCAAATTAACA+AGG - chr6_3:6635452-6635471 None:intergenic 25.0%
! TCTTCAAAAATCAGATAGAA+CGG - chr6_3:6635479-6635498 None:intergenic 25.0%
!!! TTCTATCTGATTTTTGAAGA+TGG + chr6_3:6635479-6635498 Msa0936030:intron 25.0%
!!! ATTGAATATTCTTTTTAGGC+CGG + chr6_3:6635640-6635659 Msa0936030:intron 25.0%
! AAGGATTTGATTCTTATACA+CGG - chr6_3:6635737-6635756 None:intergenic 25.0%
! CTGAATCAATAATCTTCTAA+AGG - chr6_3:6635756-6635775 None:intergenic 25.0%
! TGAATTGACAATGAATTTAG+CGG - chr6_3:6636018-6636037 None:intergenic 25.0%
! AAACAATGAGTTACTGAAAT+TGG + chr6_3:6636147-6636166 Msa0936030:intron 25.0%
! AATGAATCATTATCAGAGTT+TGG + chr6_3:6636259-6636278 Msa0936030:CDS 25.0%
! TATTTCTCTTCACAGAAATT+AGG + chr6_3:6636394-6636413 Msa0936030:three_prime_UTR 25.0%
! ATTTCTCTTCACAGAAATTA+GGG + chr6_3:6636395-6636414 Msa0936030:three_prime_UTR 25.0%
! CAATCATGAATAAAGAGAAT+AGG + chr6_3:6636429-6636448 Msa0936030:three_prime_UTR 25.0%
!!! GAAAATAATGTGTAGTTTTG+TGG + chr6_3:6636922-6636941 Msa0936030:three_prime_UTR 25.0%
ACATAAACATTGTGAAGTAG+TGG + chr6_3:6634946-6634965 Msa0936030:five_prime_UTR 30.0%
CATATTCTTCAATACAGTCT+CGG - chr6_3:6634992-6635011 None:intergenic 30.0%
! ATATCATGCATCCTTTTAGA+AGG + chr6_3:6635052-6635071 Msa0936030:CDS 30.0%
GAAATCCAAAACCTTCTAAA+AGG - chr6_3:6635066-6635085 None:intergenic 30.0%
CACGTTAATTGTAACATGTT+TGG + chr6_3:6635228-6635247 Msa0936030:intron 30.0%
! TTGTAACATGTTTGGAATCA+CGG + chr6_3:6635236-6635255 Msa0936030:intron 30.0%
ATTTGATACTTATACACTGC+CGG - chr6_3:6635662-6635681 None:intergenic 30.0%
CTGAATCAATAATCTTCAAC+AGG - chr6_3:6635685-6635704 None:intergenic 30.0%
TGGTGAGTTTGATGAAATTA+TGG + chr6_3:6635926-6635945 Msa0936030:intron 30.0%
ACATAATCAGAGAGCTATAT+AGG - chr6_3:6636124-6636143 None:intergenic 30.0%
TGAAATTGGAAAAATGTGCA+GGG + chr6_3:6636161-6636180 Msa0936030:intron 30.0%
AAGACTTCACATCATTTCTT+AGG - chr6_3:6636195-6636214 None:intergenic 30.0%
TGAAATTATTCAAATGGGCA+AGG + chr6_3:6636323-6636342 Msa0936030:CDS 30.0%
! TAGTAGGAGTGAGAATTTTA+TGG - chr6_3:6636358-6636377 None:intergenic 30.0%
!!! ATCGAGTTTTTTTCATCAGT+AGG - chr6_3:6636563-6636582 None:intergenic 30.0%
! ATTTTCATATACGATAAGGG+TGG - chr6_3:6636626-6636645 None:intergenic 30.0%
AATCATCAATTTAGTCCTTG+AGG + chr6_3:6636655-6636674 Msa0936030:three_prime_UTR 30.0%
GTTGTAAGAATCAAGAACTT+TGG + chr6_3:6636677-6636696 Msa0936030:three_prime_UTR 30.0%
TCTACAAAAGCAATACATGA+AGG - chr6_3:6636982-6637001 None:intergenic 30.0%
! AAAATCTGAGACACAATTCA+TGG - chr6_3:6637006-6637025 None:intergenic 30.0%
! AGATTTTACCTTGTATCTCT+TGG + chr6_3:6637019-6637038 Msa0936030:three_prime_UTR 30.0%
ATACAAAACCAAGAGATACA+AGG - chr6_3:6637030-6637049 None:intergenic 30.0%
ACATAAACATTGTGAAGTAG+TGG + chr6_3:6634946-6634965 Msa0936030:five_prime_UTR 30.0%
CATATTCTTCAATACAGTCT+CGG - chr6_3:6634992-6635011 None:intergenic 30.0%
! ATATCATGCATCCTTTTAGA+AGG + chr6_3:6635052-6635071 Msa0936030:CDS 30.0%
GAAATCCAAAACCTTCTAAA+AGG - chr6_3:6635066-6635085 None:intergenic 30.0%
CACGTTAATTGTAACATGTT+TGG + chr6_3:6635228-6635247 Msa0936030:intron 30.0%
! TTGTAACATGTTTGGAATCA+CGG + chr6_3:6635236-6635255 Msa0936030:intron 30.0%
ATTTGATACTTATACACTGC+CGG - chr6_3:6635662-6635681 None:intergenic 30.0%
CTGAATCAATAATCTTCAAC+AGG - chr6_3:6635685-6635704 None:intergenic 30.0%
TGGTGAGTTTGATGAAATTA+TGG + chr6_3:6635926-6635945 Msa0936030:intron 30.0%
ACATAATCAGAGAGCTATAT+AGG - chr6_3:6636124-6636143 None:intergenic 30.0%
TGAAATTGGAAAAATGTGCA+GGG + chr6_3:6636161-6636180 Msa0936030:intron 30.0%
AAGACTTCACATCATTTCTT+AGG - chr6_3:6636195-6636214 None:intergenic 30.0%
TGAAATTATTCAAATGGGCA+AGG + chr6_3:6636323-6636342 Msa0936030:CDS 30.0%
! TAGTAGGAGTGAGAATTTTA+TGG - chr6_3:6636358-6636377 None:intergenic 30.0%
!!! ATCGAGTTTTTTTCATCAGT+AGG - chr6_3:6636563-6636582 None:intergenic 30.0%
! ATTTTCATATACGATAAGGG+TGG - chr6_3:6636626-6636645 None:intergenic 30.0%
AATCATCAATTTAGTCCTTG+AGG + chr6_3:6636655-6636674 Msa0936030:three_prime_UTR 30.0%
GTTGTAAGAATCAAGAACTT+TGG + chr6_3:6636677-6636696 Msa0936030:three_prime_UTR 30.0%
TCTACAAAAGCAATACATGA+AGG - chr6_3:6636982-6637001 None:intergenic 30.0%
! AAAATCTGAGACACAATTCA+TGG - chr6_3:6637006-6637025 None:intergenic 30.0%
! AGATTTTACCTTGTATCTCT+TGG + chr6_3:6637019-6637038 Msa0936030:three_prime_UTR 30.0%
ATACAAAACCAAGAGATACA+AGG - chr6_3:6637030-6637049 None:intergenic 30.0%
CGAGACTGTATTGAAGAATA+TGG + chr6_3:6634990-6635009 Msa0936030:exon 35.0%
TGGAGAAGATGAAATTCTCT+CGG + chr6_3:6635010-6635029 Msa0936030:CDS 35.0%
!! TGCATCCTTTTAGAAGGTTT+TGG + chr6_3:6635058-6635077 Msa0936030:CDS 35.0%
TTTACGAATTCCAAAACGTG+TGG - chr6_3:6635094-6635113 None:intergenic 35.0%
!!! CGTTTTGGAATTCGTAAAAC+TGG + chr6_3:6635096-6635115 Msa0936030:CDS 35.0%
TAGTGATTCTGGTAAACTCA+TGG + chr6_3:6635362-6635381 Msa0936030:intron 35.0%
ATTTGATTCTTATACACGGC+CGG - chr6_3:6635733-6635752 None:intergenic 35.0%
TATAGCTTCTGCTAAAATCG+TGG + chr6_3:6635978-6635997 Msa0936030:intron 35.0%
CAATGAATTTAGCGGAATCA+CGG - chr6_3:6636010-6636029 None:intergenic 35.0%
ACTTGAGTGAATGCAAAGTT+TGG + chr6_3:6636066-6636085 Msa0936030:intron 35.0%
CTGAAATTGGAAAAATGTGC+AGG + chr6_3:6636160-6636179 Msa0936030:intron 35.0%
ATGAAGATATTCGTGTGATG+TGG + chr6_3:6636221-6636240 Msa0936030:CDS 35.0%
TGAAGATATTCGTGTGATGT+GGG + chr6_3:6636222-6636241 Msa0936030:CDS 35.0%
GAATCATTATCAGAGTTTGG+TGG + chr6_3:6636262-6636281 Msa0936030:CDS 35.0%
ATAGGGTTTCATGTTGAACA+TGG + chr6_3:6636447-6636466 Msa0936030:three_prime_UTR 35.0%
CAGCTCAATTGTTTCATAGT+TGG + chr6_3:6636489-6636508 Msa0936030:three_prime_UTR 35.0%
TCATCAGTAGGAATCAAACT+CGG - chr6_3:6636551-6636570 None:intergenic 35.0%
TCTTGATTCTTACAACCTCA+AGG - chr6_3:6636673-6636692 None:intergenic 35.0%
! ACAATTTAGTTCGTGCGAAA+AGG - chr6_3:6636775-6636794 None:intergenic 35.0%
ACTCAAAGACTAATGTGATC+CGG + chr6_3:6636798-6636817 Msa0936030:three_prime_UTR 35.0%
TCTTACCTACTATGTACTGT+CGG + chr6_3:6637081-6637100 Msa0936030:three_prime_UTR 35.0%
CGAGACTGTATTGAAGAATA+TGG + chr6_3:6634990-6635009 Msa0936030:exon 35.0%
TGGAGAAGATGAAATTCTCT+CGG + chr6_3:6635010-6635029 Msa0936030:CDS 35.0%
!! TGCATCCTTTTAGAAGGTTT+TGG + chr6_3:6635058-6635077 Msa0936030:CDS 35.0%
TTTACGAATTCCAAAACGTG+TGG - chr6_3:6635094-6635113 None:intergenic 35.0%
!!! CGTTTTGGAATTCGTAAAAC+TGG + chr6_3:6635096-6635115 Msa0936030:CDS 35.0%
TAGTGATTCTGGTAAACTCA+TGG + chr6_3:6635362-6635381 Msa0936030:intron 35.0%
ATTTGATTCTTATACACGGC+CGG - chr6_3:6635733-6635752 None:intergenic 35.0%
TATAGCTTCTGCTAAAATCG+TGG + chr6_3:6635978-6635997 Msa0936030:intron 35.0%
CAATGAATTTAGCGGAATCA+CGG - chr6_3:6636010-6636029 None:intergenic 35.0%
ACTTGAGTGAATGCAAAGTT+TGG + chr6_3:6636066-6636085 Msa0936030:intron 35.0%
CTGAAATTGGAAAAATGTGC+AGG + chr6_3:6636160-6636179 Msa0936030:intron 35.0%
ATGAAGATATTCGTGTGATG+TGG + chr6_3:6636221-6636240 Msa0936030:CDS 35.0%
TGAAGATATTCGTGTGATGT+GGG + chr6_3:6636222-6636241 Msa0936030:CDS 35.0%
GAATCATTATCAGAGTTTGG+TGG + chr6_3:6636262-6636281 Msa0936030:CDS 35.0%
ATAGGGTTTCATGTTGAACA+TGG + chr6_3:6636447-6636466 Msa0936030:three_prime_UTR 35.0%
CAGCTCAATTGTTTCATAGT+TGG + chr6_3:6636489-6636508 Msa0936030:three_prime_UTR 35.0%
TCATCAGTAGGAATCAAACT+CGG - chr6_3:6636551-6636570 None:intergenic 35.0%
TCTTGATTCTTACAACCTCA+AGG - chr6_3:6636673-6636692 None:intergenic 35.0%
! ACAATTTAGTTCGTGCGAAA+AGG - chr6_3:6636775-6636794 None:intergenic 35.0%
ACTCAAAGACTAATGTGATC+CGG + chr6_3:6636798-6636817 Msa0936030:three_prime_UTR 35.0%
TCTTACCTACTATGTACTGT+CGG + chr6_3:6637081-6637100 Msa0936030:three_prime_UTR 35.0%
TTCTTCAATACAGTCTCGGT+AGG - chr6_3:6634988-6635007 None:intergenic 40.0%
! ATTTCCATTGCCACACGTTT+TGG + chr6_3:6635081-6635100 Msa0936030:CDS 40.0%
AATTCCAAAACGTGTGGCAA+TGG - chr6_3:6635088-6635107 None:intergenic 40.0%
TTGTAGCGTGTTTGAATTGC+TGG + chr6_3:6635906-6635925 Msa0936030:intron 40.0%
! TCATTATCAGAGTTTGGTGG+TGG + chr6_3:6636265-6636284 Msa0936030:CDS 40.0%
GACTCACACACATGATTAGT+AGG - chr6_3:6636374-6636393 None:intergenic 40.0%
TAAGGGTGGCTACATCAAAT+TGG - chr6_3:6636612-6636631 None:intergenic 40.0%
TTCGTGCGAAAAGGTTCTAT+AGG - chr6_3:6636766-6636785 None:intergenic 40.0%
CTCAAAGACTAATGTGATCC+GGG + chr6_3:6636799-6636818 Msa0936030:three_prime_UTR 40.0%
TCAAAGACTAATGTGATCCG+GGG + chr6_3:6636800-6636819 Msa0936030:three_prime_UTR 40.0%
! TGTAACCGAAATTTTCTCCC+CGG - chr6_3:6636820-6636839 None:intergenic 40.0%
TTACACCGACAGTACATAGT+AGG - chr6_3:6637089-6637108 None:intergenic 40.0%
TTCTTCAATACAGTCTCGGT+AGG - chr6_3:6634988-6635007 None:intergenic 40.0%
! ATTTCCATTGCCACACGTTT+TGG + chr6_3:6635081-6635100 Msa0936030:CDS 40.0%
AATTCCAAAACGTGTGGCAA+TGG - chr6_3:6635088-6635107 None:intergenic 40.0%
TTGTAGCGTGTTTGAATTGC+TGG + chr6_3:6635906-6635925 Msa0936030:intron 40.0%
! TCATTATCAGAGTTTGGTGG+TGG + chr6_3:6636265-6636284 Msa0936030:CDS 40.0%
GACTCACACACATGATTAGT+AGG - chr6_3:6636374-6636393 None:intergenic 40.0%
TAAGGGTGGCTACATCAAAT+TGG - chr6_3:6636612-6636631 None:intergenic 40.0%
TTCGTGCGAAAAGGTTCTAT+AGG - chr6_3:6636766-6636785 None:intergenic 40.0%
CTCAAAGACTAATGTGATCC+GGG + chr6_3:6636799-6636818 Msa0936030:three_prime_UTR 40.0%
TCAAAGACTAATGTGATCCG+GGG + chr6_3:6636800-6636819 Msa0936030:three_prime_UTR 40.0%
! TGTAACCGAAATTTTCTCCC+CGG - chr6_3:6636820-6636839 None:intergenic 40.0%
TTACACCGACAGTACATAGT+AGG - chr6_3:6637089-6637108 None:intergenic 40.0%
GAAGATGAAATTCTCTCGGC+AGG + chr6_3:6635014-6635033 Msa0936030:CDS 45.0%
ACCACGACTAATTACATGCG+TGG + chr6_3:6635553-6635572 Msa0936030:intron 45.0%
!! CGTGGCTTTAGAAGTAGTTG+TGG + chr6_3:6635571-6635590 Msa0936030:intron 45.0%
GTGATCCGGGGAGAAAATTT+CGG + chr6_3:6636812-6636831 Msa0936030:three_prime_UTR 45.0%
GAAGATGAAATTCTCTCGGC+AGG + chr6_3:6635014-6635033 Msa0936030:CDS 45.0%
ACCACGACTAATTACATGCG+TGG + chr6_3:6635553-6635572 Msa0936030:intron 45.0%
!! CGTGGCTTTAGAAGTAGTTG+TGG + chr6_3:6635571-6635590 Msa0936030:intron 45.0%
GTGATCCGGGGAGAAAATTT+CGG + chr6_3:6636812-6636831 Msa0936030:three_prime_UTR 45.0%
GATGAAATTCTCTCGGCAGG+TGG + chr6_3:6635017-6635036 Msa0936030:CDS 50.0%
GCCACGCATGTAATTAGTCG+TGG - chr6_3:6635557-6635576 None:intergenic 50.0%
GGGTGGCTACATCAAATTGG+TGG - chr6_3:6636609-6636628 None:intergenic 50.0%
GATGAAATTCTCTCGGCAGG+TGG + chr6_3:6635017-6635036 Msa0936030:CDS 50.0%
GCCACGCATGTAATTAGTCG+TGG - chr6_3:6635557-6635576 None:intergenic 50.0%
GGGTGGCTACATCAAATTGG+TGG - chr6_3:6636609-6636628 None:intergenic 50.0%
TCTCTCGGCAGGTGGAGTTG+TGG + chr6_3:6635025-6635044 Msa0936030:CDS 60.0%
TCTCTCGGCAGGTGGAGTTG+TGG + chr6_3:6635025-6635044 Msa0936030:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 6634771 6637110 6634771 ID=Msa0936030;Name=Msa0936030
chr6_3 mRNA 6634771 6637110 6634771 ID=Msa0936030-mRNA-1;Parent=Msa0936030;Name=Msa0936030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=238|1|1|1|1|1|2|750|95
chr6_3 exon 6634771 6635117 6634771 ID=Msa0936030-mRNA-1:exon:2100;Parent=Msa0936030-mRNA-1
chr6_3 exon 6636182 6637110 6636182 ID=Msa0936030-mRNA-1:exon:2101;Parent=Msa0936030-mRNA-1
chr6_3 five_prime_UTR 6634771 6635008 6634771 ID=Msa0936030-mRNA-1:five_prime_utr;Parent=Msa0936030-mRNA-1
chr6_3 CDS 6635009 6635117 6635009 ID=Msa0936030-mRNA-1:cds;Parent=Msa0936030-mRNA-1
chr6_3 CDS 6636182 6636360 6636182 ID=Msa0936030-mRNA-1:cds;Parent=Msa0936030-mRNA-1
chr6_3 three_prime_UTR 6636361 6637110 6636361 ID=Msa0936030-mRNA-1:three_prime_utr;Parent=Msa0936030-mRNA-1
Gene Sequence

>Msa0936030

ATGGAGAAGATGAAATTCTCTCGGCAGGTGGAGTTGTGGCAGAATATCATGCATCCTTTTAGAAGGTTTTGGATTTCCATTGCCACACGTTTTGGAATTCGTAAAACTGGGTTGAAGAACCTAAGAAATGATGTGAAGTCTTGTGAATATGAAGATATTCGTGTGATGTGGGAGATGCTAAACAGAAATGAATCATTATCAGAGTTTGGTGGTGGTAATTCTCCAAACAAAATCAAGAAAATTCATTACTTGAAATTATTCAAATGGGCAAGGTGTGCTGCTCCATAA
Protein sequence

>Msa0936030

MEKMKFSRQVELWQNIMHPFRRFWISIATRFGIRKTGLKNLRNDVKSCEYEDIRVMWEMLNRNESLSEFGGGNSPNKIKKIHYLKLFKWARCAAP*