AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1336950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1336950 MtrunA17_Chr6g0451831 93.684 95 6 0 1 95 1 95 2.70e-63 186
Msa1336950 MtrunA17_Chr2g0334081 41.379 58 32 1 10 65 1 58 6.86e-13 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1336950 AT5G43150.1 42.188 64 37 0 4 67 1 64 1.86e-12 58.2

Find 49 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAAACATCTACAATTTA+AGG 0.057799 tig0001334:+71939 Msa1336950:three_prime_UTR
TATTTCTCTTCACAGAAATT+AGG 0.147148 tig0001334:+71454 Msa1336950:three_prime_UTR
ATTTATTTCAGAAACCATTT+TGG 0.186799 tig0001334:-71780 None:intergenic
AAGACTTCACATCATTTCTT+AGG 0.245992 tig0001334:-71252 None:intergenic
CGAGACTGTATTGAAGAATA+TGG 0.303346 tig0001334:+70169 Msa1336950:exon
AAATTAACAAAATTCCAAAA+TGG 0.316346 tig0001334:+71766 Msa1336950:three_prime_UTR
ATTTCTCTTCACAGAAATTA+GGG 0.322081 tig0001334:+71455 Msa1336950:three_prime_UTR
AATGAATCATTATCAGAGTT+TGG 0.349611 tig0001334:+71319 Msa1336950:CDS
AGTACTTGAAATTATTCAAA+TGG 0.355186 tig0001334:+71377 Msa1336950:CDS
GTGATCCGGGGAGAAAATTT+CGG 0.381711 tig0001334:+71872 Msa1336950:three_prime_UTR
CAGCTCAATTGTTTCGTAGT+TGG 0.393155 tig0001334:+71549 Msa1336950:three_prime_UTR
TAAGGGTGGCTACATCAAAT+TGG 0.395071 tig0001334:-71669 None:intergenic
TTCTCTTTATTCATGATTGA+AGG 0.411458 tig0001334:-71485 None:intergenic
TATTCTACCAAGTACATAGT+AGG 0.428178 tig0001334:-72146 None:intergenic
TCAATCATGAATAAAGAGAA+TGG 0.441154 tig0001334:+71488 Msa1336950:three_prime_UTR
CAATCATGAATAAAGAGAAT+GGG 0.445925 tig0001334:+71489 Msa1336950:three_prime_UTR
GTACTTGAAATTATTCAAAT+GGG 0.452163 tig0001334:+71378 Msa1336950:CDS
ACAATTTAGTTCGTGCGAAA+AGG 0.463261 tig0001334:-71832 None:intergenic
AAAATCTGAGACACAATTCA+TGG 0.464808 tig0001334:-72063 None:intergenic
ACTCAAAGACTAATGTGATC+CGG 0.466511 tig0001334:+71858 Msa1336950:three_prime_UTR
GTTGTAAGAATCAAGAACTT+TGG 0.472962 tig0001334:+71737 Msa1336950:three_prime_UTR
CTCAAAGACTAATGTGATCC+GGG 0.475469 tig0001334:+71859 Msa1336950:three_prime_UTR
GAAGATGAAATTCTCTCGGC+AGG 0.497923 tig0001334:+70193 Msa1336950:CDS
GAAATCCAAAACCTTCGAAA+AGG 0.500736 tig0001334:-70242 None:intergenic
GACTCACACACATGATTAGT+AGG 0.504425 tig0001334:-71431 None:intergenic
AATTCTAAAACGTGTGGCAA+TGG 0.520815 tig0001334:-70264 None:intergenic
TTCGTGCGAAAAGGTTCTAT+AGG 0.523082 tig0001334:-71823 None:intergenic
TGAAATTATTCAAATGGGCA+AGG 0.527275 tig0001334:+71383 Msa1336950:CDS
TATCTTACCTACTATGTACT+TGG 0.546238 tig0001334:+72139 Msa1336950:three_prime_UTR
AATCATCAATTTAGTCCTTG+AGG 0.549847 tig0001334:+71715 Msa1336950:three_prime_UTR
TCTTGATTCTTACAACCTCA+AGG 0.555331 tig0001334:-71730 None:intergenic
TCATTATCAGAGTTTGGTGG+TGG 0.560367 tig0001334:+71325 Msa1336950:CDS
CATATTCTTCAATACAGTCT+CGG 0.572911 tig0001334:-70168 None:intergenic
TTTACGAATTCTAAAACGTG+TGG 0.573490 tig0001334:-70270 None:intergenic
ATGACGATATTCGTGTGATG+TGG 0.576669 tig0001334:+71281 Msa1336950:CDS
GGGTGGCTACATCAAATTGG+TGG 0.578105 tig0001334:-71666 None:intergenic
TGGAGAAGATGAAATTCTCT+CGG 0.589361 tig0001334:+70189 Msa1336950:CDS
TCTACAAAAGCAATACATGA+AGG 0.590440 tig0001334:-72039 None:intergenic
TCTCTCGGCAGGTGGAGTTG+TGG 0.592580 tig0001334:+70204 Msa1336950:CDS
AATCATGAATAAAGAGAATG+GGG 0.601705 tig0001334:+71490 Msa1336950:three_prime_UTR
GATGAAATTCTCTCGGCAGG+TGG 0.607611 tig0001334:+70196 Msa1336950:CDS
ATGGGGTTTCATGTTGAACA+TGG 0.629590 tig0001334:+71507 Msa1336950:three_prime_UTR
ATACAAAACCAAGAGATACA+AGG 0.637518 tig0001334:-72087 None:intergenic
TTCTTCAATACAGTCTCGGT+AGG 0.640844 tig0001334:-70164 None:intergenic
GAATCATTATCAGAGTTTGG+TGG 0.650506 tig0001334:+71322 Msa1336950:CDS
TGACGATATTCGTGTGATGT+GGG 0.650750 tig0001334:+71282 Msa1336950:CDS
TCATCAGTAGGAATCAAACT+CGG 0.659777 tig0001334:-71608 None:intergenic
ATAAGCATTGTGAAGTGTAG+TGG 0.668618 tig0001334:+70125 Msa1336950:five_prime_UTR
TCAAAGACTAATGTGATCCG+GGG 0.689237 tig0001334:+71860 Msa1336950:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTACTATTAATTGTTG+TGG + tig0001334:70081-70100 Msa1336950:five_prime_UTR 15.0%
!!! TTCACATTGAATATTTTTTT+AGG + tig0001334:70820-70839 Msa1336950:intron 15.0%
!! AAATTAACAAAATTCCAAAA+TGG + tig0001334:71766-71785 Msa1336950:three_prime_UTR 15.0%
!!! TATTTTACTATTAATTGTTG+TGG + tig0001334:70081-70100 Msa1336950:five_prime_UTR 15.0%
!!! TTCACATTGAATATTTTTTT+AGG + tig0001334:70820-70839 Msa1336950:intron 15.0%
!! AAATTAACAAAATTCCAAAA+TGG + tig0001334:71766-71785 Msa1336950:three_prime_UTR 15.0%
!!! CTAAGATATTTTTCATTAGA+CGG + tig0001334:70000-70019 Msa1336950:five_prime_UTR 20.0%
!!! TAGTTTTATCAAAAGAAAGA+TGG + tig0001334:70531-70550 Msa1336950:intron 20.0%
!!! TTGAAATAAACTTGTTAAGT+TGG + tig0001334:70636-70655 Msa1336950:intron 20.0%
!!! TGAAATAAACTTGTTAAGTT+GGG + tig0001334:70637-70656 Msa1336950:intron 20.0%
!!! GTTTAATTTTAATACACTGA+CGG + tig0001334:70708-70727 Msa1336950:intron 20.0%
!! TATCCACATATAATTAATCA+TGG - tig0001334:70762-70781 None:intergenic 20.0%
!!! TCATCAAAATCAAGATTTTA+AGG - tig0001334:70891-70910 None:intergenic 20.0%
!!! TTGATTTTGATGATTATTTG+TGG + tig0001334:70898-70917 Msa1336950:intron 20.0%
!! TTGGAATAAACATGTTAATT+TGG + tig0001334:71145-71164 Msa1336950:intron 20.0%
!! AGTACTTGAAATTATTCAAA+TGG + tig0001334:71377-71396 Msa1336950:CDS 20.0%
!! GTACTTGAAATTATTCAAAT+GGG + tig0001334:71378-71397 Msa1336950:CDS 20.0%
!!! GTTATTTTCATATACGATAA+GGG - tig0001334:71689-71708 None:intergenic 20.0%
!!! AGTTATTTTCATATACGATA+AGG - tig0001334:71690-71709 None:intergenic 20.0%
!!! TTGGAATTTTGTTAATTTCA+AGG - tig0001334:71764-71783 None:intergenic 20.0%
!!! ATTTATTTCAGAAACCATTT+TGG - tig0001334:71783-71802 None:intergenic 20.0%
!!! TTTTGAATTTTGTCAATTTC+AGG + tig0001334:71908-71927 Msa1336950:three_prime_UTR 20.0%
!!! TTTGAATTTTGTCAATTTCA+GGG + tig0001334:71909-71928 Msa1336950:three_prime_UTR 20.0%
!! TTTCAAACATCTACAATTTA+AGG + tig0001334:71939-71958 Msa1336950:three_prime_UTR 20.0%
!!! CTAAGATATTTTTCATTAGA+CGG + tig0001334:70000-70019 Msa1336950:five_prime_UTR 20.0%
!!! TAGTTTTATCAAAAGAAAGA+TGG + tig0001334:70531-70550 Msa1336950:intron 20.0%
!!! TTGAAATAAACTTGTTAAGT+TGG + tig0001334:70636-70655 Msa1336950:intron 20.0%
!!! TGAAATAAACTTGTTAAGTT+GGG + tig0001334:70637-70656 Msa1336950:intron 20.0%
!!! GTTTAATTTTAATACACTGA+CGG + tig0001334:70708-70727 Msa1336950:intron 20.0%
!! TATCCACATATAATTAATCA+TGG - tig0001334:70762-70781 None:intergenic 20.0%
!!! TCATCAAAATCAAGATTTTA+AGG - tig0001334:70891-70910 None:intergenic 20.0%
!!! TTGATTTTGATGATTATTTG+TGG + tig0001334:70898-70917 Msa1336950:intron 20.0%
!! TTGGAATAAACATGTTAATT+TGG + tig0001334:71145-71164 Msa1336950:intron 20.0%
!! AGTACTTGAAATTATTCAAA+TGG + tig0001334:71377-71396 Msa1336950:CDS 20.0%
!! GTACTTGAAATTATTCAAAT+GGG + tig0001334:71378-71397 Msa1336950:CDS 20.0%
!!! GTTATTTTCATATACGATAA+GGG - tig0001334:71689-71708 None:intergenic 20.0%
!!! AGTTATTTTCATATACGATA+AGG - tig0001334:71690-71709 None:intergenic 20.0%
!!! TTGGAATTTTGTTAATTTCA+AGG - tig0001334:71764-71783 None:intergenic 20.0%
!!! ATTTATTTCAGAAACCATTT+TGG - tig0001334:71783-71802 None:intergenic 20.0%
!!! TTTTGAATTTTGTCAATTTC+AGG + tig0001334:71908-71927 Msa1336950:three_prime_UTR 20.0%
!!! TTTGAATTTTGTCAATTTCA+GGG + tig0001334:71909-71928 Msa1336950:three_prime_UTR 20.0%
!! TTTCAAACATCTACAATTTA+AGG + tig0001334:71939-71958 Msa1336950:three_prime_UTR 20.0%
! TTTGTTACAAAGTACATGTT+TGG + tig0001334:70336-70355 Msa1336950:intron 25.0%
! CACGTTAATTGTAACATATT+TGG + tig0001334:70405-70424 Msa1336950:intron 25.0%
! GTGAATTGTTGTAACATATT+TGG + tig0001334:70463-70482 Msa1336950:intron 25.0%
!!! CATTGAATATTTTTTTAGGC+CGG + tig0001334:70824-70843 Msa1336950:intron 25.0%
! TGAATTGACAATGAATTTAG+CGG - tig0001334:71078-71097 None:intergenic 25.0%
! AAACAATGAGTTACTGAAAT+TGG + tig0001334:71207-71226 Msa1336950:intron 25.0%
! AATGAATCATTATCAGAGTT+TGG + tig0001334:71319-71338 Msa1336950:CDS 25.0%
!! TGAATTTTCTTGATTTTGCT+TGG - tig0001334:71358-71377 None:intergenic 25.0%
! TATTTCTCTTCACAGAAATT+AGG + tig0001334:71454-71473 Msa1336950:three_prime_UTR 25.0%
! ATTTCTCTTCACAGAAATTA+GGG + tig0001334:71455-71474 Msa1336950:three_prime_UTR 25.0%
! TTCTCTTTATTCATGATTGA+AGG - tig0001334:71488-71507 None:intergenic 25.0%
! TCAATCATGAATAAAGAGAA+TGG + tig0001334:71488-71507 Msa1336950:three_prime_UTR 25.0%
! CAATCATGAATAAAGAGAAT+GGG + tig0001334:71489-71508 Msa1336950:three_prime_UTR 25.0%
! AATCATGAATAAAGAGAATG+GGG + tig0001334:71490-71509 Msa1336950:three_prime_UTR 25.0%
!!! GAAAATAATGTGTAGTTTTG+TGG + tig0001334:71982-72001 Msa1336950:three_prime_UTR 25.0%
! TTTGTTACAAAGTACATGTT+TGG + tig0001334:70336-70355 Msa1336950:intron 25.0%
! CACGTTAATTGTAACATATT+TGG + tig0001334:70405-70424 Msa1336950:intron 25.0%
! GTGAATTGTTGTAACATATT+TGG + tig0001334:70463-70482 Msa1336950:intron 25.0%
!!! CATTGAATATTTTTTTAGGC+CGG + tig0001334:70824-70843 Msa1336950:intron 25.0%
! TGAATTGACAATGAATTTAG+CGG - tig0001334:71078-71097 None:intergenic 25.0%
! AAACAATGAGTTACTGAAAT+TGG + tig0001334:71207-71226 Msa1336950:intron 25.0%
! AATGAATCATTATCAGAGTT+TGG + tig0001334:71319-71338 Msa1336950:CDS 25.0%
!! TGAATTTTCTTGATTTTGCT+TGG - tig0001334:71358-71377 None:intergenic 25.0%
! TATTTCTCTTCACAGAAATT+AGG + tig0001334:71454-71473 Msa1336950:three_prime_UTR 25.0%
! ATTTCTCTTCACAGAAATTA+GGG + tig0001334:71455-71474 Msa1336950:three_prime_UTR 25.0%
! TTCTCTTTATTCATGATTGA+AGG - tig0001334:71488-71507 None:intergenic 25.0%
! TCAATCATGAATAAAGAGAA+TGG + tig0001334:71488-71507 Msa1336950:three_prime_UTR 25.0%
! CAATCATGAATAAAGAGAAT+GGG + tig0001334:71489-71508 Msa1336950:three_prime_UTR 25.0%
! AATCATGAATAAAGAGAATG+GGG + tig0001334:71490-71509 Msa1336950:three_prime_UTR 25.0%
!!! GAAAATAATGTGTAGTTTTG+TGG + tig0001334:71982-72001 Msa1336950:three_prime_UTR 25.0%
CATATTCTTCAATACAGTCT+CGG - tig0001334:70171-70190 None:intergenic 30.0%
!! TTTACGAATTCTAAAACGTG+TGG - tig0001334:70273-70292 None:intergenic 30.0%
!!! CGTTTTAGAATTCGTAAAAC+TGG + tig0001334:70275-70294 Msa1336950:CDS 30.0%
AAAAATCAGACAGAACTACA+AGG - tig0001334:70679-70698 None:intergenic 30.0%
!! TTCTGTCTGATTTTTGAAGA+TGG + tig0001334:70684-70703 Msa1336950:intron 30.0%
! GACGGTGTAAACAATTTTTA+CGG + tig0001334:70726-70745 Msa1336950:intron 30.0%
! AACAATTTTTACGGCATTAG+AGG + tig0001334:70735-70754 Msa1336950:intron 30.0%
GGACCATGATTAATTATATG+TGG + tig0001334:70756-70775 Msa1336950:intron 30.0%
ATTTGATTCTTATACACTGC+CGG - tig0001334:70846-70865 None:intergenic 30.0%
TGGTGAGTTTGATGAAATTA+TGG + tig0001334:70986-71005 Msa1336950:intron 30.0%
ACATAATCAGAGAGCTATAT+AGG - tig0001334:71184-71203 None:intergenic 30.0%
TGAAATTGGAAAAATGTGCA+GGG + tig0001334:71221-71240 Msa1336950:intron 30.0%
AAGACTTCACATCATTTCTT+AGG - tig0001334:71255-71274 None:intergenic 30.0%
TGAAATTATTCAAATGGGCA+AGG + tig0001334:71383-71402 Msa1336950:CDS 30.0%
! TAGTAGGAGTGAGAATTTTA+TGG - tig0001334:71418-71437 None:intergenic 30.0%
!!! ATCGAGTTTTTTTCATCAGT+AGG - tig0001334:71623-71642 None:intergenic 30.0%
! ATTTTCATATACGATAAGGG+TGG - tig0001334:71686-71705 None:intergenic 30.0%
AATCATCAATTTAGTCCTTG+AGG + tig0001334:71715-71734 Msa1336950:three_prime_UTR 30.0%
GTTGTAAGAATCAAGAACTT+TGG + tig0001334:71737-71756 Msa1336950:three_prime_UTR 30.0%
TCTACAAAAGCAATACATGA+AGG - tig0001334:72042-72061 None:intergenic 30.0%
! AAAATCTGAGACACAATTCA+TGG - tig0001334:72066-72085 None:intergenic 30.0%
! AGATTTTACCTTGTATCTCT+TGG + tig0001334:72079-72098 Msa1336950:three_prime_UTR 30.0%
ATACAAAACCAAGAGATACA+AGG - tig0001334:72090-72109 None:intergenic 30.0%
TATCTTACCTACTATGTACT+TGG + tig0001334:72139-72158 Msa1336950:three_prime_UTR 30.0%
TATTCTACCAAGTACATAGT+AGG - tig0001334:72149-72168 None:intergenic 30.0%
CATATTCTTCAATACAGTCT+CGG - tig0001334:70171-70190 None:intergenic 30.0%
!! TTTACGAATTCTAAAACGTG+TGG - tig0001334:70273-70292 None:intergenic 30.0%
!!! CGTTTTAGAATTCGTAAAAC+TGG + tig0001334:70275-70294 Msa1336950:CDS 30.0%
AAAAATCAGACAGAACTACA+AGG - tig0001334:70679-70698 None:intergenic 30.0%
!! TTCTGTCTGATTTTTGAAGA+TGG + tig0001334:70684-70703 Msa1336950:intron 30.0%
! GACGGTGTAAACAATTTTTA+CGG + tig0001334:70726-70745 Msa1336950:intron 30.0%
! AACAATTTTTACGGCATTAG+AGG + tig0001334:70735-70754 Msa1336950:intron 30.0%
GGACCATGATTAATTATATG+TGG + tig0001334:70756-70775 Msa1336950:intron 30.0%
ATTTGATTCTTATACACTGC+CGG - tig0001334:70846-70865 None:intergenic 30.0%
TGGTGAGTTTGATGAAATTA+TGG + tig0001334:70986-71005 Msa1336950:intron 30.0%
ACATAATCAGAGAGCTATAT+AGG - tig0001334:71184-71203 None:intergenic 30.0%
TGAAATTGGAAAAATGTGCA+GGG + tig0001334:71221-71240 Msa1336950:intron 30.0%
AAGACTTCACATCATTTCTT+AGG - tig0001334:71255-71274 None:intergenic 30.0%
TGAAATTATTCAAATGGGCA+AGG + tig0001334:71383-71402 Msa1336950:CDS 30.0%
! TAGTAGGAGTGAGAATTTTA+TGG - tig0001334:71418-71437 None:intergenic 30.0%
!!! ATCGAGTTTTTTTCATCAGT+AGG - tig0001334:71623-71642 None:intergenic 30.0%
! ATTTTCATATACGATAAGGG+TGG - tig0001334:71686-71705 None:intergenic 30.0%
AATCATCAATTTAGTCCTTG+AGG + tig0001334:71715-71734 Msa1336950:three_prime_UTR 30.0%
GTTGTAAGAATCAAGAACTT+TGG + tig0001334:71737-71756 Msa1336950:three_prime_UTR 30.0%
TCTACAAAAGCAATACATGA+AGG - tig0001334:72042-72061 None:intergenic 30.0%
! AAAATCTGAGACACAATTCA+TGG - tig0001334:72066-72085 None:intergenic 30.0%
! AGATTTTACCTTGTATCTCT+TGG + tig0001334:72079-72098 Msa1336950:three_prime_UTR 30.0%
ATACAAAACCAAGAGATACA+AGG - tig0001334:72090-72109 None:intergenic 30.0%
TATCTTACCTACTATGTACT+TGG + tig0001334:72139-72158 Msa1336950:three_prime_UTR 30.0%
TATTCTACCAAGTACATAGT+AGG - tig0001334:72149-72168 None:intergenic 30.0%
! ATAAGCATTGTGAAGTGTAG+TGG + tig0001334:70125-70144 Msa1336950:five_prime_UTR 35.0%
CGAGACTGTATTGAAGAATA+TGG + tig0001334:70169-70188 Msa1336950:exon 35.0%
TGGAGAAGATGAAATTCTCT+CGG + tig0001334:70189-70208 Msa1336950:CDS 35.0%
GAAATCCAAAACCTTCGAAA+AGG - tig0001334:70245-70264 None:intergenic 35.0%
!! AATTCTAAAACGTGTGGCAA+TGG - tig0001334:70267-70286 None:intergenic 35.0%
CACGAGTAGTTTGACAAAAT+CGG + tig0001334:70431-70450 Msa1336950:intron 35.0%
GAGTTTAGCAGAATCATGAT+CGG - tig0001334:70557-70576 None:intergenic 35.0%
GCTGAACAATAATCTTCAGA+AGG - tig0001334:70869-70888 None:intergenic 35.0%
TATAGCTTCTGCTAAAATCG+TGG + tig0001334:71038-71057 Msa1336950:intron 35.0%
CAATGAATTTAGCGGAATCA+TGG - tig0001334:71070-71089 None:intergenic 35.0%
ACTTGAGTGAATGCAAAGTT+TGG + tig0001334:71126-71145 Msa1336950:intron 35.0%
CTGAAATTGGAAAAATGTGC+AGG + tig0001334:71220-71239 Msa1336950:intron 35.0%
GAATCATTATCAGAGTTTGG+TGG + tig0001334:71322-71341 Msa1336950:CDS 35.0%
TCATCAGTAGGAATCAAACT+CGG - tig0001334:71611-71630 None:intergenic 35.0%
TCTTGATTCTTACAACCTCA+AGG - tig0001334:71733-71752 None:intergenic 35.0%
! ACAATTTAGTTCGTGCGAAA+AGG - tig0001334:71835-71854 None:intergenic 35.0%
ACTCAAAGACTAATGTGATC+CGG + tig0001334:71858-71877 Msa1336950:three_prime_UTR 35.0%
! ATAAGCATTGTGAAGTGTAG+TGG + tig0001334:70125-70144 Msa1336950:five_prime_UTR 35.0%
CGAGACTGTATTGAAGAATA+TGG + tig0001334:70169-70188 Msa1336950:exon 35.0%
TGGAGAAGATGAAATTCTCT+CGG + tig0001334:70189-70208 Msa1336950:CDS 35.0%
GAAATCCAAAACCTTCGAAA+AGG - tig0001334:70245-70264 None:intergenic 35.0%
!! AATTCTAAAACGTGTGGCAA+TGG - tig0001334:70267-70286 None:intergenic 35.0%
CACGAGTAGTTTGACAAAAT+CGG + tig0001334:70431-70450 Msa1336950:intron 35.0%
GAGTTTAGCAGAATCATGAT+CGG - tig0001334:70557-70576 None:intergenic 35.0%
GCTGAACAATAATCTTCAGA+AGG - tig0001334:70869-70888 None:intergenic 35.0%
TATAGCTTCTGCTAAAATCG+TGG + tig0001334:71038-71057 Msa1336950:intron 35.0%
CAATGAATTTAGCGGAATCA+TGG - tig0001334:71070-71089 None:intergenic 35.0%
ACTTGAGTGAATGCAAAGTT+TGG + tig0001334:71126-71145 Msa1336950:intron 35.0%
CTGAAATTGGAAAAATGTGC+AGG + tig0001334:71220-71239 Msa1336950:intron 35.0%
GAATCATTATCAGAGTTTGG+TGG + tig0001334:71322-71341 Msa1336950:CDS 35.0%
TCATCAGTAGGAATCAAACT+CGG - tig0001334:71611-71630 None:intergenic 35.0%
TCTTGATTCTTACAACCTCA+AGG - tig0001334:71733-71752 None:intergenic 35.0%
! ACAATTTAGTTCGTGCGAAA+AGG - tig0001334:71835-71854 None:intergenic 35.0%
ACTCAAAGACTAATGTGATC+CGG + tig0001334:71858-71877 Msa1336950:three_prime_UTR 35.0%
TTCTTCAATACAGTCTCGGT+AGG - tig0001334:70167-70186 None:intergenic 40.0%
! ACATCATGCATCCTTTTCGA+AGG + tig0001334:70231-70250 Msa1336950:CDS 40.0%
! TGCATCCTTTTCGAAGGTTT+TGG + tig0001334:70237-70256 Msa1336950:CDS 40.0%
TTGTAGCGTGTTTGAATTGC+TGG + tig0001334:70966-70985 Msa1336950:intron 40.0%
ATGACGATATTCGTGTGATG+TGG + tig0001334:71281-71300 Msa1336950:CDS 40.0%
TGACGATATTCGTGTGATGT+GGG + tig0001334:71282-71301 Msa1336950:CDS 40.0%
! TCATTATCAGAGTTTGGTGG+TGG + tig0001334:71325-71344 Msa1336950:CDS 40.0%
GACTCACACACATGATTAGT+AGG - tig0001334:71434-71453 None:intergenic 40.0%
ATGGGGTTTCATGTTGAACA+TGG + tig0001334:71507-71526 Msa1336950:three_prime_UTR 40.0%
CAGCTCAATTGTTTCGTAGT+TGG + tig0001334:71549-71568 Msa1336950:three_prime_UTR 40.0%
TAAGGGTGGCTACATCAAAT+TGG - tig0001334:71672-71691 None:intergenic 40.0%
TTCGTGCGAAAAGGTTCTAT+AGG - tig0001334:71826-71845 None:intergenic 40.0%
CTCAAAGACTAATGTGATCC+GGG + tig0001334:71859-71878 Msa1336950:three_prime_UTR 40.0%
TCAAAGACTAATGTGATCCG+GGG + tig0001334:71860-71879 Msa1336950:three_prime_UTR 40.0%
! TGTAACCGAAATTTTCTCCC+CGG - tig0001334:71880-71899 None:intergenic 40.0%
TTCTTCAATACAGTCTCGGT+AGG - tig0001334:70167-70186 None:intergenic 40.0%
! ACATCATGCATCCTTTTCGA+AGG + tig0001334:70231-70250 Msa1336950:CDS 40.0%
! TGCATCCTTTTCGAAGGTTT+TGG + tig0001334:70237-70256 Msa1336950:CDS 40.0%
TTGTAGCGTGTTTGAATTGC+TGG + tig0001334:70966-70985 Msa1336950:intron 40.0%
ATGACGATATTCGTGTGATG+TGG + tig0001334:71281-71300 Msa1336950:CDS 40.0%
TGACGATATTCGTGTGATGT+GGG + tig0001334:71282-71301 Msa1336950:CDS 40.0%
! TCATTATCAGAGTTTGGTGG+TGG + tig0001334:71325-71344 Msa1336950:CDS 40.0%
GACTCACACACATGATTAGT+AGG - tig0001334:71434-71453 None:intergenic 40.0%
ATGGGGTTTCATGTTGAACA+TGG + tig0001334:71507-71526 Msa1336950:three_prime_UTR 40.0%
CAGCTCAATTGTTTCGTAGT+TGG + tig0001334:71549-71568 Msa1336950:three_prime_UTR 40.0%
TAAGGGTGGCTACATCAAAT+TGG - tig0001334:71672-71691 None:intergenic 40.0%
TTCGTGCGAAAAGGTTCTAT+AGG - tig0001334:71826-71845 None:intergenic 40.0%
CTCAAAGACTAATGTGATCC+GGG + tig0001334:71859-71878 Msa1336950:three_prime_UTR 40.0%
TCAAAGACTAATGTGATCCG+GGG + tig0001334:71860-71879 Msa1336950:three_prime_UTR 40.0%
! TGTAACCGAAATTTTCTCCC+CGG - tig0001334:71880-71899 None:intergenic 40.0%
GAAGATGAAATTCTCTCGGC+AGG + tig0001334:70193-70212 Msa1336950:CDS 45.0%
GTGATCCGGGGAGAAAATTT+CGG + tig0001334:71872-71891 Msa1336950:three_prime_UTR 45.0%
GAAGATGAAATTCTCTCGGC+AGG + tig0001334:70193-70212 Msa1336950:CDS 45.0%
GTGATCCGGGGAGAAAATTT+CGG + tig0001334:71872-71891 Msa1336950:three_prime_UTR 45.0%
GATGAAATTCTCTCGGCAGG+TGG + tig0001334:70196-70215 Msa1336950:CDS 50.0%
GGGTGGCTACATCAAATTGG+TGG - tig0001334:71669-71688 None:intergenic 50.0%
GATGAAATTCTCTCGGCAGG+TGG + tig0001334:70196-70215 Msa1336950:CDS 50.0%
GGGTGGCTACATCAAATTGG+TGG - tig0001334:71669-71688 None:intergenic 50.0%
TCTCTCGGCAGGTGGAGTTG+TGG + tig0001334:70204-70223 Msa1336950:CDS 60.0%
TCTCTCGGCAGGTGGAGTTG+TGG + tig0001334:70204-70223 Msa1336950:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0001334 gene 69953 72195 69953 ID=Msa1336950;Name=Msa1336950
tig0001334 mRNA 69953 72195 69953 ID=Msa1336950-mRNA-1;Parent=Msa1336950;Name=Msa1336950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=235|1|1|1|1|1|2|775|95
tig0001334 exon 69953 70296 69953 ID=Msa1336950-mRNA-1:exon:917;Parent=Msa1336950-mRNA-1
tig0001334 exon 71242 72195 71242 ID=Msa1336950-mRNA-1:exon:918;Parent=Msa1336950-mRNA-1
tig0001334 five_prime_UTR 69953 70187 69953 ID=Msa1336950-mRNA-1:five_prime_utr;Parent=Msa1336950-mRNA-1
tig0001334 CDS 70188 70296 70188 ID=Msa1336950-mRNA-1:cds;Parent=Msa1336950-mRNA-1
tig0001334 CDS 71242 71420 71242 ID=Msa1336950-mRNA-1:cds;Parent=Msa1336950-mRNA-1
tig0001334 three_prime_UTR 71421 72195 71421 ID=Msa1336950-mRNA-1:three_prime_utr;Parent=Msa1336950-mRNA-1
Gene Sequence

>Msa1336950

ATGGAGAAGATGAAATTCTCTCGGCAGGTGGAGTTGTGGCAGAACATCATGCATCCTTTTCGAAGGTTTTGGATTTCCATTGCCACACGTTTTAGAATTCGTAAAACTGGGTTGAAGAACCTAAGAAATGATGTGAAGTCTTGTGAATATGACGATATTCGTGTGATGTGGGAGATGCTAAACAGAAATGAATCATTATCAGAGTTTGGTGGTGGTAATTCTCCAAGCAAAATCAAGAAAATTCAGTACTTGAAATTATTCAAATGGGCAAGGTGTGCTGCTCCATAA
Protein sequence

>Msa1336950

MEKMKFSRQVELWQNIMHPFRRFWISIATRFRIRKTGLKNLRNDVKSCEYDDIRVMWEMLNRNESLSEFGGGNSPSKIKKIQYLKLFKWARCAAP*