AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0879010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0879010 MtrunA17_Chr6g0461701 95.946 148 6 0 1 148 165 312 2.60e-91 267
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0879010 AT5G17570.1 63.014 146 49 1 1 146 164 304 2.63e-56 179
Msa0879010 AT5G17570.2 63.014 146 49 1 1 146 164 304 3.17e-56 178
Msa0879010 AT3G03500.2 47.260 146 37 2 1 146 130 235 1.86e-32 115
Msa0879010 AT3G03500.1 47.260 146 37 2 1 146 164 269 1.97e-32 116

Find 34 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAATTCTTATTGTTATTA+TGG 0.169330 6_1:+20516218 Msa0879010:five_prime_UTR
CGTAGATTCTCTTTCTCTAA+TGG 0.276541 6_1:+20516254 Msa0879010:five_prime_UTR
TCTCTTGACAATGTGCTTCA+TGG 0.320207 6_1:+20516695 Msa0879010:CDS
CTATTTAGGTCTCTTCTGAT+AGG 0.353528 6_1:+20516594 Msa0879010:intron
TATGTCACTAAAAGCTAATA+AGG 0.393389 6_1:+20516463 Msa0879010:CDS
AGAATAAGCGAACTGCATTC+TGG 0.394096 6_1:-20517720 None:intergenic
GTTATCCTTCATTCTTACCT+AGG 0.423711 6_1:+20516377 Msa0879010:five_prime_UTR
TTCGACTTTGGTAGTGCATC+AGG 0.424489 6_1:-20516638 None:intergenic
CTCTTCTGATAGGATGTTGT+TGG 0.443450 6_1:+20516604 Msa0879010:CDS
GTTCGCTTATTCTCCTATGA+AGG 0.470681 6_1:+20517730 Msa0879010:CDS
AGAGAATCTATGTTCGACTT+TGG 0.477960 6_1:-20516650 None:intergenic
TTCTAAATGTTCCAGGTCTG+TGG 0.496419 6_1:+20516337 Msa0879010:five_prime_UTR
TATGCTAGAGATTACAAAAG+AGG 0.500520 6_1:+20517678 Msa0879010:CDS
TTACCTAGGTTCAGCTGAGA+TGG 0.504944 6_1:+20516391 Msa0879010:exon
GGATGGTTGAGTGTATCTTT+CGG 0.517161 6_1:-20516797 None:intergenic
GTAACATCGAAGCATCAGTT+AGG 0.519550 6_1:-20516775 None:intergenic
GAAAGATACACTCAACCATC+CGG 0.523721 6_1:+20516799 Msa0879010:CDS
AGGATAACACCAGCAGGAAA+AGG 0.528313 6_1:-20516362 None:intergenic
TCGAAGCATCAGTTAGGACT+TGG 0.529353 6_1:-20516769 None:intergenic
CGTTATGAATGTTAGCCGGA+TGG 0.532725 6_1:-20516814 None:intergenic
AGGACTTGGGACGAATTAGC+GGG 0.533334 6_1:-20516755 None:intergenic
GGCGAAGAAAATGCTGAAGA+TGG 0.551302 6_1:+20516484 Msa0879010:CDS
GAATGAAGGATAACACCAGC+AGG 0.557862 6_1:-20516368 None:intergenic
TAGGACTTGGGACGAATTAG+CGG 0.558442 6_1:-20516756 None:intergenic
CTTTCTCTAATGGAATGCAA+TGG 0.561664 6_1:+20516264 Msa0879010:five_prime_UTR
TCTAAATGTTCCAGGTCTGT+GGG 0.580026 6_1:+20516338 Msa0879010:five_prime_UTR
CTGAACCTAGGTAAGAATGA+AGG 0.581265 6_1:-20516382 None:intergenic
AGGAAAAGGTCCCACAGACC+TGG 0.582898 6_1:-20516348 None:intergenic
GGGAGAAGAATCAGAAGTTG+AGG 0.586811 6_1:-20516735 None:intergenic
GCACCAAGCTTTGAAAACTC+AGG 0.593705 6_1:-20516416 None:intergenic
CTTACGTTATGAATGTTAGC+CGG 0.594181 6_1:-20516818 None:intergenic
GAACCATCTCAGCTGAACCT+AGG 0.613905 6_1:-20516394 None:intergenic
CGAAGCATCAGTTAGGACTT+GGG 0.625448 6_1:-20516768 None:intergenic
TGACATAAGAAATCCAGAGA+AGG 0.627443 6_1:-20516447 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATTAAAAAAAACATAT+GGG - chr6_1:20516208-20516227 None:intergenic 10.0%
!! TAAGAATTAAAAAAAACATA+TGG - chr6_1:20516209-20516228 None:intergenic 10.0%
!!! TTTAATTCTTATTGTTATTA+TGG + chr6_1:20516218-20516237 Msa0879010:five_prime_UTR 10.0%
!! GTAAAAGTAAATTTAAAAAA+TGG - chr6_1:20517492-20517511 None:intergenic 10.0%
!! AAGAATTAAAAAAAACATAT+GGG - chr6_1:20516208-20516227 None:intergenic 10.0%
!! TAAGAATTAAAAAAAACATA+TGG - chr6_1:20516209-20516228 None:intergenic 10.0%
!!! TTTAATTCTTATTGTTATTA+TGG + chr6_1:20516218-20516237 Msa0879010:five_prime_UTR 10.0%
!! GTAAAAGTAAATTTAAAAAA+TGG - chr6_1:20517492-20517511 None:intergenic 10.0%
!! TCTTATATTCTATTCTATTT+AGG + chr6_1:20516580-20516599 Msa0879010:intron 15.0%
!!! AGAAAATGCAATTTTTTTTT+TGG - chr6_1:20517103-20517122 None:intergenic 15.0%
!! TAAATTTAAAAAATGGGAAA+CGG - chr6_1:20517485-20517504 None:intergenic 15.0%
!! TTCAAAAATAAACAAATTAC+AGG + chr6_1:20517764-20517783 Msa0879010:exon 15.0%
!! TCTTATATTCTATTCTATTT+AGG + chr6_1:20516580-20516599 Msa0879010:intron 15.0%
!!! AGAAAATGCAATTTTTTTTT+TGG - chr6_1:20517103-20517122 None:intergenic 15.0%
!! TAAATTTAAAAAATGGGAAA+CGG - chr6_1:20517485-20517504 None:intergenic 15.0%
!! TTCAAAAATAAACAAATTAC+AGG + chr6_1:20517764-20517783 Msa0879010:exon 15.0%
!!! TGGAACATTTAGAATTTTTA+AGG - chr6_1:20516331-20516350 None:intergenic 20.0%
!! TTAAAAATTCTAAATGTTCC+AGG + chr6_1:20516330-20516349 Msa0879010:five_prime_UTR 20.0%
!!! AAGAGATGATTATTTTGAAA+GGG - chr6_1:20516876-20516895 None:intergenic 20.0%
!!! TAAGAGATGATTATTTTGAA+AGG - chr6_1:20516877-20516896 None:intergenic 20.0%
!!! AAATGCAATTTTTTTTTTGG+TGG - chr6_1:20517100-20517119 None:intergenic 20.0%
!!! CATCTTAAATTGCAATTTTA+GGG + chr6_1:20517330-20517349 Msa0879010:intron 20.0%
!!! TGGAACATTTAGAATTTTTA+AGG - chr6_1:20516331-20516350 None:intergenic 20.0%
!! TTAAAAATTCTAAATGTTCC+AGG + chr6_1:20516330-20516349 Msa0879010:five_prime_UTR 20.0%
!!! AAGAGATGATTATTTTGAAA+GGG - chr6_1:20516876-20516895 None:intergenic 20.0%
!!! TAAGAGATGATTATTTTGAA+AGG - chr6_1:20516877-20516896 None:intergenic 20.0%
!!! AAATGCAATTTTTTTTTTGG+TGG - chr6_1:20517100-20517119 None:intergenic 20.0%
!!! CATCTTAAATTGCAATTTTA+GGG + chr6_1:20517330-20517349 Msa0879010:intron 20.0%
! TATGTCACTAAAAGCTAATA+AGG + chr6_1:20516463-20516482 Msa0879010:CDS 25.0%
! TCTTGATATAGTTTCATGTA+TGG + chr6_1:20517179-20517198 Msa0879010:intron 25.0%
!! AGCATTACATTTTCATACTT+CGG - chr6_1:20517286-20517305 None:intergenic 25.0%
! CCTAAAATTGCAATTTAAGA+TGG - chr6_1:20517332-20517351 None:intergenic 25.0%
!! CCATCTTAAATTGCAATTTT+AGG + chr6_1:20517329-20517348 Msa0879010:intron 25.0%
! TCCATGTCATTATATTCATT+CGG - chr6_1:20517557-20517576 None:intergenic 25.0%
!!! CAGAATGATGTTTTAAAGAT+CGG - chr6_1:20517603-20517622 None:intergenic 25.0%
!! CTTGATTTCATTTTACATAC+AGG + chr6_1:20517636-20517655 Msa0879010:intron 25.0%
!!! AAGTAATTTTGATCCTTCAT+AGG - chr6_1:20517746-20517765 None:intergenic 25.0%
! TATGTCACTAAAAGCTAATA+AGG + chr6_1:20516463-20516482 Msa0879010:CDS 25.0%
! TCTTGATATAGTTTCATGTA+TGG + chr6_1:20517179-20517198 Msa0879010:intron 25.0%
!! AGCATTACATTTTCATACTT+CGG - chr6_1:20517286-20517305 None:intergenic 25.0%
! CCTAAAATTGCAATTTAAGA+TGG - chr6_1:20517332-20517351 None:intergenic 25.0%
!! CCATCTTAAATTGCAATTTT+AGG + chr6_1:20517329-20517348 Msa0879010:intron 25.0%
! TCCATGTCATTATATTCATT+CGG - chr6_1:20517557-20517576 None:intergenic 25.0%
!!! CAGAATGATGTTTTAAAGAT+CGG - chr6_1:20517603-20517622 None:intergenic 25.0%
!! CTTGATTTCATTTTACATAC+AGG + chr6_1:20517636-20517655 Msa0879010:intron 25.0%
!!! AAGTAATTTTGATCCTTCAT+AGG - chr6_1:20517746-20517765 None:intergenic 25.0%
! GATTCTCTTTTCTTTGTTGA+AGG + chr6_1:20516665-20516684 Msa0879010:CDS 30.0%
AAAGGGAAAAGTGACAATAA+AGG - chr6_1:20516859-20516878 None:intergenic 30.0%
CATCTCTTATATCATCAACT+TGG + chr6_1:20516888-20516907 Msa0879010:intron 30.0%
! GGGAAAAAAAAATGGTTTTC+TGG + chr6_1:20516968-20516987 Msa0879010:intron 30.0%
GACAATGCATAAAATATGCA+AGG + chr6_1:20517052-20517071 Msa0879010:intron 30.0%
!!! TGCAATTTTTTTTTTGGTGG+CGG - chr6_1:20517097-20517116 None:intergenic 30.0%
!!! TAGGTTTTGAAAGCTTCAAA+GGG - chr6_1:20517126-20517145 None:intergenic 30.0%
GATATAGTTTCATGTATGGA+AGG + chr6_1:20517183-20517202 Msa0879010:intron 30.0%
! TTTGAAACAAATTCACCAGT+AGG + chr6_1:20517209-20517228 Msa0879010:intron 30.0%
ATGAGAACAACTTTCCTTAT+GGG - chr6_1:20517243-20517262 None:intergenic 30.0%
! GCATTACATTTTCATACTTC+GGG - chr6_1:20517285-20517304 None:intergenic 30.0%
!! AATTGCAATTTTAGGGATGA+AGG + chr6_1:20517337-20517356 Msa0879010:intron 30.0%
CCATGTCATTATATTCATTC+GGG - chr6_1:20517556-20517575 None:intergenic 30.0%
TATGCTAGAGATTACAAAAG+AGG + chr6_1:20517678-20517697 Msa0879010:CDS 30.0%
! GATTCTCTTTTCTTTGTTGA+AGG + chr6_1:20516665-20516684 Msa0879010:CDS 30.0%
AAAGGGAAAAGTGACAATAA+AGG - chr6_1:20516859-20516878 None:intergenic 30.0%
CATCTCTTATATCATCAACT+TGG + chr6_1:20516888-20516907 Msa0879010:intron 30.0%
! GGGAAAAAAAAATGGTTTTC+TGG + chr6_1:20516968-20516987 Msa0879010:intron 30.0%
GACAATGCATAAAATATGCA+AGG + chr6_1:20517052-20517071 Msa0879010:intron 30.0%
!!! TGCAATTTTTTTTTTGGTGG+CGG - chr6_1:20517097-20517116 None:intergenic 30.0%
!!! TAGGTTTTGAAAGCTTCAAA+GGG - chr6_1:20517126-20517145 None:intergenic 30.0%
GATATAGTTTCATGTATGGA+AGG + chr6_1:20517183-20517202 Msa0879010:intron 30.0%
! TTTGAAACAAATTCACCAGT+AGG + chr6_1:20517209-20517228 Msa0879010:intron 30.0%
ATGAGAACAACTTTCCTTAT+GGG - chr6_1:20517243-20517262 None:intergenic 30.0%
! GCATTACATTTTCATACTTC+GGG - chr6_1:20517285-20517304 None:intergenic 30.0%
!! AATTGCAATTTTAGGGATGA+AGG + chr6_1:20517337-20517356 Msa0879010:intron 30.0%
CCATGTCATTATATTCATTC+GGG - chr6_1:20517556-20517575 None:intergenic 30.0%
TATGCTAGAGATTACAAAAG+AGG + chr6_1:20517678-20517697 Msa0879010:CDS 30.0%
CGTAGATTCTCTTTCTCTAA+TGG + chr6_1:20516254-20516273 Msa0879010:five_prime_UTR 35.0%
CTTTCTCTAATGGAATGCAA+TGG + chr6_1:20516264-20516283 Msa0879010:five_prime_UTR 35.0%
GTTATCCTTCATTCTTACCT+AGG + chr6_1:20516377-20516396 Msa0879010:five_prime_UTR 35.0%
TGACATAAGAAATCCAGAGA+AGG - chr6_1:20516450-20516469 None:intergenic 35.0%
CTATTTAGGTCTCTTCTGAT+AGG + chr6_1:20516594-20516613 Msa0879010:intron 35.0%
AGAGAATCTATGTTCGACTT+TGG - chr6_1:20516653-20516672 None:intergenic 35.0%
CTTACGTTATGAATGTTAGC+CGG - chr6_1:20516821-20516840 None:intergenic 35.0%
GCTAACATTCATAACGTAAG+TGG + chr6_1:20516821-20516840 Msa0879010:intron 35.0%
TGCATAAAATATGCAAGGTC+TGG + chr6_1:20517057-20517076 Msa0879010:intron 35.0%
GCATAAAATATGCAAGGTCT+GGG + chr6_1:20517058-20517077 Msa0879010:intron 35.0%
CATAAAATATGCAAGGTCTG+GGG + chr6_1:20517059-20517078 Msa0879010:intron 35.0%
!!! CTAGGTTTTGAAAGCTTCAA+AGG - chr6_1:20517127-20517146 None:intergenic 35.0%
TGAGAACAACTTTCCTTATG+GGG - chr6_1:20517242-20517261 None:intergenic 35.0%
CATGAGAACAACTTTCCTTA+TGG - chr6_1:20517244-20517263 None:intergenic 35.0%
AATGCTATCTTCAATGCATG+AGG + chr6_1:20517300-20517319 Msa0879010:intron 35.0%
ATGCTATCTTCAATGCATGA+GGG + chr6_1:20517301-20517320 Msa0879010:intron 35.0%
CCCGAATGAATATAATGACA+TGG + chr6_1:20517553-20517572 Msa0879010:intron 35.0%
CGTAGATTCTCTTTCTCTAA+TGG + chr6_1:20516254-20516273 Msa0879010:five_prime_UTR 35.0%
CTTTCTCTAATGGAATGCAA+TGG + chr6_1:20516264-20516283 Msa0879010:five_prime_UTR 35.0%
GTTATCCTTCATTCTTACCT+AGG + chr6_1:20516377-20516396 Msa0879010:five_prime_UTR 35.0%
TGACATAAGAAATCCAGAGA+AGG - chr6_1:20516450-20516469 None:intergenic 35.0%
CTATTTAGGTCTCTTCTGAT+AGG + chr6_1:20516594-20516613 Msa0879010:intron 35.0%
AGAGAATCTATGTTCGACTT+TGG - chr6_1:20516653-20516672 None:intergenic 35.0%
CTTACGTTATGAATGTTAGC+CGG - chr6_1:20516821-20516840 None:intergenic 35.0%
GCTAACATTCATAACGTAAG+TGG + chr6_1:20516821-20516840 Msa0879010:intron 35.0%
TGCATAAAATATGCAAGGTC+TGG + chr6_1:20517057-20517076 Msa0879010:intron 35.0%
GCATAAAATATGCAAGGTCT+GGG + chr6_1:20517058-20517077 Msa0879010:intron 35.0%
CATAAAATATGCAAGGTCTG+GGG + chr6_1:20517059-20517078 Msa0879010:intron 35.0%
!!! CTAGGTTTTGAAAGCTTCAA+AGG - chr6_1:20517127-20517146 None:intergenic 35.0%
TGAGAACAACTTTCCTTATG+GGG - chr6_1:20517242-20517261 None:intergenic 35.0%
CATGAGAACAACTTTCCTTA+TGG - chr6_1:20517244-20517263 None:intergenic 35.0%
AATGCTATCTTCAATGCATG+AGG + chr6_1:20517300-20517319 Msa0879010:intron 35.0%
ATGCTATCTTCAATGCATGA+GGG + chr6_1:20517301-20517320 Msa0879010:intron 35.0%
CCCGAATGAATATAATGACA+TGG + chr6_1:20517553-20517572 Msa0879010:intron 35.0%
TTCTAAATGTTCCAGGTCTG+TGG + chr6_1:20516337-20516356 Msa0879010:five_prime_UTR 40.0%
TCTAAATGTTCCAGGTCTGT+GGG + chr6_1:20516338-20516357 Msa0879010:five_prime_UTR 40.0%
CTGAACCTAGGTAAGAATGA+AGG - chr6_1:20516385-20516404 None:intergenic 40.0%
! GTTCCTGAGTTTTCAAAGCT+TGG + chr6_1:20516413-20516432 Msa0879010:CDS 40.0%
!! GGTGCTTATTTTTCCTTCTC+TGG + chr6_1:20516434-20516453 Msa0879010:CDS 40.0%
CTCTTCTGATAGGATGTTGT+TGG + chr6_1:20516604-20516623 Msa0879010:CDS 40.0%
TCTCTTGACAATGTGCTTCA+TGG + chr6_1:20516695-20516714 Msa0879010:CDS 40.0%
! GTAACATCGAAGCATCAGTT+AGG - chr6_1:20516778-20516797 None:intergenic 40.0%
GGATGGTTGAGTGTATCTTT+CGG - chr6_1:20516800-20516819 None:intergenic 40.0%
GAAAGATACACTCAACCATC+CGG + chr6_1:20516799-20516818 Msa0879010:CDS 40.0%
! TTAGTTTGAGCTTGCTCTCT+GGG + chr6_1:20516947-20516966 Msa0879010:intron 40.0%
! GCTCTCTGGGGAAAAAAAAA+TGG + chr6_1:20516960-20516979 Msa0879010:intron 40.0%
!!! ATTTTTTTTTTGGTGGCGGC+CGG - chr6_1:20517093-20517112 None:intergenic 40.0%
TTATGGGGAAAGAAACCTAC+TGG - chr6_1:20517227-20517246 None:intergenic 40.0%
AGTAGGTTTCTTTCCCCATA+AGG + chr6_1:20517226-20517245 Msa0879010:intron 40.0%
!! GCAATTTTAGGGATGAAGGA+TGG + chr6_1:20517341-20517360 Msa0879010:intron 40.0%
TAATGACATGGAAGTCTCAC+TGG + chr6_1:20517565-20517584 Msa0879010:intron 40.0%
!! AAGTCTCACTGGTTTGGTAT+CGG + chr6_1:20517576-20517595 Msa0879010:intron 40.0%
AGAATAAGCGAACTGCATTC+TGG - chr6_1:20517723-20517742 None:intergenic 40.0%
GTTCGCTTATTCTCCTATGA+AGG + chr6_1:20517730-20517749 Msa0879010:CDS 40.0%
TTCTAAATGTTCCAGGTCTG+TGG + chr6_1:20516337-20516356 Msa0879010:five_prime_UTR 40.0%
TCTAAATGTTCCAGGTCTGT+GGG + chr6_1:20516338-20516357 Msa0879010:five_prime_UTR 40.0%
CTGAACCTAGGTAAGAATGA+AGG - chr6_1:20516385-20516404 None:intergenic 40.0%
! GTTCCTGAGTTTTCAAAGCT+TGG + chr6_1:20516413-20516432 Msa0879010:CDS 40.0%
!! GGTGCTTATTTTTCCTTCTC+TGG + chr6_1:20516434-20516453 Msa0879010:CDS 40.0%
CTCTTCTGATAGGATGTTGT+TGG + chr6_1:20516604-20516623 Msa0879010:CDS 40.0%
TCTCTTGACAATGTGCTTCA+TGG + chr6_1:20516695-20516714 Msa0879010:CDS 40.0%
! GTAACATCGAAGCATCAGTT+AGG - chr6_1:20516778-20516797 None:intergenic 40.0%
GGATGGTTGAGTGTATCTTT+CGG - chr6_1:20516800-20516819 None:intergenic 40.0%
GAAAGATACACTCAACCATC+CGG + chr6_1:20516799-20516818 Msa0879010:CDS 40.0%
! TTAGTTTGAGCTTGCTCTCT+GGG + chr6_1:20516947-20516966 Msa0879010:intron 40.0%
! GCTCTCTGGGGAAAAAAAAA+TGG + chr6_1:20516960-20516979 Msa0879010:intron 40.0%
!!! ATTTTTTTTTTGGTGGCGGC+CGG - chr6_1:20517093-20517112 None:intergenic 40.0%
TTATGGGGAAAGAAACCTAC+TGG - chr6_1:20517227-20517246 None:intergenic 40.0%
AGTAGGTTTCTTTCCCCATA+AGG + chr6_1:20517226-20517245 Msa0879010:intron 40.0%
!! GCAATTTTAGGGATGAAGGA+TGG + chr6_1:20517341-20517360 Msa0879010:intron 40.0%
TAATGACATGGAAGTCTCAC+TGG + chr6_1:20517565-20517584 Msa0879010:intron 40.0%
!! AAGTCTCACTGGTTTGGTAT+CGG + chr6_1:20517576-20517595 Msa0879010:intron 40.0%
AGAATAAGCGAACTGCATTC+TGG - chr6_1:20517723-20517742 None:intergenic 40.0%
GTTCGCTTATTCTCCTATGA+AGG + chr6_1:20517730-20517749 Msa0879010:CDS 40.0%
AGGATAACACCAGCAGGAAA+AGG - chr6_1:20516365-20516384 None:intergenic 45.0%
GAATGAAGGATAACACCAGC+AGG - chr6_1:20516371-20516390 None:intergenic 45.0%
TTACCTAGGTTCAGCTGAGA+TGG + chr6_1:20516391-20516410 Msa0879010:exon 45.0%
!! GCACCAAGCTTTGAAAACTC+AGG - chr6_1:20516419-20516438 None:intergenic 45.0%
GGCGAAGAAAATGCTGAAGA+TGG + chr6_1:20516484-20516503 Msa0879010:CDS 45.0%
!! TTCGACTTTGGTAGTGCATC+AGG - chr6_1:20516641-20516660 None:intergenic 45.0%
GGGAGAAGAATCAGAAGTTG+AGG - chr6_1:20516738-20516757 None:intergenic 45.0%
TAGGACTTGGGACGAATTAG+CGG - chr6_1:20516759-20516778 None:intergenic 45.0%
! CGAAGCATCAGTTAGGACTT+GGG - chr6_1:20516771-20516790 None:intergenic 45.0%
! TCGAAGCATCAGTTAGGACT+TGG - chr6_1:20516772-20516791 None:intergenic 45.0%
CGTTATGAATGTTAGCCGGA+TGG - chr6_1:20516817-20516836 None:intergenic 45.0%
CAGAGAGCAAGCTCAAACTA+AGG - chr6_1:20516948-20516967 None:intergenic 45.0%
! CTTAGTTTGAGCTTGCTCTC+TGG + chr6_1:20516946-20516965 Msa0879010:intron 45.0%
! TAGTTTGAGCTTGCTCTCTG+GGG + chr6_1:20516948-20516967 Msa0879010:intron 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG + chr6_1:20517030-20517049 Msa0879010:intron 45.0%
!!! TTTTTTTTTTGGTGGCGGCC+GGG - chr6_1:20517092-20517111 None:intergenic 45.0%
ACTAGAAGCAGCTGAGAACT+AGG - chr6_1:20517145-20517164 None:intergenic 45.0%
AAACCAACCAGCAAACTAGC+CGG - chr6_1:20517434-20517453 None:intergenic 45.0%
TTAGCCAGACTGCAATACGA+CGG - chr6_1:20517462-20517481 None:intergenic 45.0%
! ACATGGAAGTCTCACTGGTT+TGG + chr6_1:20517570-20517589 Msa0879010:intron 45.0%
AGGATAACACCAGCAGGAAA+AGG - chr6_1:20516365-20516384 None:intergenic 45.0%
GAATGAAGGATAACACCAGC+AGG - chr6_1:20516371-20516390 None:intergenic 45.0%
TTACCTAGGTTCAGCTGAGA+TGG + chr6_1:20516391-20516410 Msa0879010:exon 45.0%
!! GCACCAAGCTTTGAAAACTC+AGG - chr6_1:20516419-20516438 None:intergenic 45.0%
GGCGAAGAAAATGCTGAAGA+TGG + chr6_1:20516484-20516503 Msa0879010:CDS 45.0%
!! TTCGACTTTGGTAGTGCATC+AGG - chr6_1:20516641-20516660 None:intergenic 45.0%
GGGAGAAGAATCAGAAGTTG+AGG - chr6_1:20516738-20516757 None:intergenic 45.0%
TAGGACTTGGGACGAATTAG+CGG - chr6_1:20516759-20516778 None:intergenic 45.0%
! CGAAGCATCAGTTAGGACTT+GGG - chr6_1:20516771-20516790 None:intergenic 45.0%
! TCGAAGCATCAGTTAGGACT+TGG - chr6_1:20516772-20516791 None:intergenic 45.0%
CGTTATGAATGTTAGCCGGA+TGG - chr6_1:20516817-20516836 None:intergenic 45.0%
CAGAGAGCAAGCTCAAACTA+AGG - chr6_1:20516948-20516967 None:intergenic 45.0%
! CTTAGTTTGAGCTTGCTCTC+TGG + chr6_1:20516946-20516965 Msa0879010:intron 45.0%
! TAGTTTGAGCTTGCTCTCTG+GGG + chr6_1:20516948-20516967 Msa0879010:intron 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG + chr6_1:20517030-20517049 Msa0879010:intron 45.0%
!!! TTTTTTTTTTGGTGGCGGCC+GGG - chr6_1:20517092-20517111 None:intergenic 45.0%
ACTAGAAGCAGCTGAGAACT+AGG - chr6_1:20517145-20517164 None:intergenic 45.0%
AAACCAACCAGCAAACTAGC+CGG - chr6_1:20517434-20517453 None:intergenic 45.0%
TTAGCCAGACTGCAATACGA+CGG - chr6_1:20517462-20517481 None:intergenic 45.0%
! ACATGGAAGTCTCACTGGTT+TGG + chr6_1:20517570-20517589 Msa0879010:intron 45.0%
!! TAAAAGTAAATTTAAAAAAT+GGG - chr6_1:20517491-20517510 None:intergenic 5.0%
!! TAAAAGTAAATTTAAAAAAT+GGG - chr6_1:20517491-20517510 None:intergenic 5.0%
GAACCATCTCAGCTGAACCT+AGG - chr6_1:20516397-20516416 None:intergenic 50.0%
AGGACTTGGGACGAATTAGC+GGG - chr6_1:20516758-20516777 None:intergenic 50.0%
CAACTGAGCTAAGCTCACGA+GGG - chr6_1:20517027-20517046 None:intergenic 50.0%
!!! TTTTTTTTTGGTGGCGGCCG+GGG - chr6_1:20517091-20517110 None:intergenic 50.0%
! CAACGAGTTGAACCGAGAAC+CGG + chr6_1:20517412-20517431 Msa0879010:intron 50.0%
TAGCCAGACTGCAATACGAC+GGG - chr6_1:20517461-20517480 None:intergenic 50.0%
GAACCATCTCAGCTGAACCT+AGG - chr6_1:20516397-20516416 None:intergenic 50.0%
AGGACTTGGGACGAATTAGC+GGG - chr6_1:20516758-20516777 None:intergenic 50.0%
CAACTGAGCTAAGCTCACGA+GGG - chr6_1:20517027-20517046 None:intergenic 50.0%
!!! TTTTTTTTTGGTGGCGGCCG+GGG - chr6_1:20517091-20517110 None:intergenic 50.0%
! CAACGAGTTGAACCGAGAAC+CGG + chr6_1:20517412-20517431 Msa0879010:intron 50.0%
TAGCCAGACTGCAATACGAC+GGG - chr6_1:20517461-20517480 None:intergenic 50.0%
AGGAAAAGGTCCCACAGACC+TGG - chr6_1:20516351-20516370 None:intergenic 55.0%
! TCTGTGGGACCTTTTCCTGC+TGG + chr6_1:20516353-20516372 Msa0879010:five_prime_UTR 55.0%
CCAACTGAGCTAAGCTCACG+AGG - chr6_1:20517028-20517047 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG + chr6_1:20517025-20517044 Msa0879010:intron 55.0%
! AAGGTCTGGGGTTCAAACCC+CGG + chr6_1:20517071-20517090 Msa0879010:intron 55.0%
! CCAGCAAACTAGCCGGTTCT+CGG - chr6_1:20517427-20517446 None:intergenic 55.0%
!! GAACCGGCTAGTTTGCTGGT+TGG + chr6_1:20517428-20517447 Msa0879010:intron 55.0%
AGCCAGACTGCAATACGACG+GGG - chr6_1:20517460-20517479 None:intergenic 55.0%
AGGAAAAGGTCCCACAGACC+TGG - chr6_1:20516351-20516370 None:intergenic 55.0%
! TCTGTGGGACCTTTTCCTGC+TGG + chr6_1:20516353-20516372 Msa0879010:five_prime_UTR 55.0%
CCAACTGAGCTAAGCTCACG+AGG - chr6_1:20517028-20517047 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG + chr6_1:20517025-20517044 Msa0879010:intron 55.0%
! AAGGTCTGGGGTTCAAACCC+CGG + chr6_1:20517071-20517090 Msa0879010:intron 55.0%
! CCAGCAAACTAGCCGGTTCT+CGG - chr6_1:20517427-20517446 None:intergenic 55.0%
!! GAACCGGCTAGTTTGCTGGT+TGG + chr6_1:20517428-20517447 Msa0879010:intron 55.0%
AGCCAGACTGCAATACGACG+GGG - chr6_1:20517460-20517479 None:intergenic 55.0%
!! CCGAGAACCGGCTAGTTTGC+TGG + chr6_1:20517424-20517443 Msa0879010:intron 60.0%
ACCCCCGTCGTATTGCAGTC+TGG + chr6_1:20517455-20517474 Msa0879010:intron 60.0%
GCCAGACTGCAATACGACGG+GGG - chr6_1:20517459-20517478 None:intergenic 60.0%
!! CCGAGAACCGGCTAGTTTGC+TGG + chr6_1:20517424-20517443 Msa0879010:intron 60.0%
ACCCCCGTCGTATTGCAGTC+TGG + chr6_1:20517455-20517474 Msa0879010:intron 60.0%
GCCAGACTGCAATACGACGG+GGG - chr6_1:20517459-20517478 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 20516201 20517887 20516201 ID=Msa0879010;Name=Msa0879010
chr6_1 mRNA 20516201 20517887 20516201 ID=Msa0879010-mRNA-1;Parent=Msa0879010;Name=Msa0879010-mRNA-1;_AED=0.06;_eAED=0.06;_QI=209|1|1|1|1|1|3|113|148
chr6_1 exon 20516201 20516505 20516201 ID=Msa0879010-mRNA-1:exon:5575;Parent=Msa0879010-mRNA-1
chr6_1 exon 20516602 20516835 20516602 ID=Msa0879010-mRNA-1:exon:5576;Parent=Msa0879010-mRNA-1
chr6_1 exon 20517658 20517887 20517658 ID=Msa0879010-mRNA-1:exon:5577;Parent=Msa0879010-mRNA-1
chr6_1 five_prime_UTR 20516201 20516409 20516201 ID=Msa0879010-mRNA-1:five_prime_utr;Parent=Msa0879010-mRNA-1
chr6_1 CDS 20516410 20516505 20516410 ID=Msa0879010-mRNA-1:cds;Parent=Msa0879010-mRNA-1
chr6_1 CDS 20516602 20516835 20516602 ID=Msa0879010-mRNA-1:cds;Parent=Msa0879010-mRNA-1
chr6_1 CDS 20517658 20517774 20517658 ID=Msa0879010-mRNA-1:cds;Parent=Msa0879010-mRNA-1
chr6_1 three_prime_UTR 20517775 20517887 20517775 ID=Msa0879010-mRNA-1:three_prime_utr;Parent=Msa0879010-mRNA-1
Gene Sequence

>Msa0879010

ATGGTTCCTGAGTTTTCAAAGCTTGGTGCTTATTTTTCCTTCTCTGGATTTCTTATGTCACTAAAAGCTAATAAGGCGAAGAAAATGCTGAAGATGGTCTCTTCTGATAGGATGTTGTTGGAGACAGATGCACCTGATGCACTACCAAAGTCGAACATAGATTCTCTTTTCTTTGTTGAAGGAGATACTTCTCTTGACAATGTGCTTCATGGACAAAGAACAACTTCATCCTCAACTTCTGATTCTTCTCCCGCTAATTCGTCCCAAGTCCTAACTGATGCTTCGATGTTACCGAAAGATACACTCAACCATCCGGCTAACATTCATAACGTACTTGATTATGTTGCATCTATGCTAGAGATTACAAAAGAGGAACTAGCTGAATTAAGTTACCAGAATGCAGTTCGCTTATTCTCCTATGAAGGATCAAAATTACTTCAAAAATAA
Protein sequence

>Msa0879010

MVPEFSKLGAYFSFSGFLMSLKANKAKKMLKMVSSDRMLLETDAPDALPKSNIDSLFFVEGDTSLDNVLHGQRTTSSSTSDSSPANSSQVLTDASMLPKDTLNHPANIHNVLDYVASMLEITKEELAELSYQNAVRLFSYEGSKLLQK*