Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0883360 | KEH22422.1 | 81.111 | 90 | 12 | 1 | 21 | 105 | 1 | 90 | 2.81e-42 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0883360 | A0A072TYC8 | 81.111 | 90 | 12 | 1 | 21 | 105 | 1 | 90 | 1.34e-42 | 144 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0211680 | Msa0883360 | 0.803368 | 3.605089e-49 | -8.615850e-47 |
| Msa0883360 | Msa1092990 | 0.802202 | 6.275542e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAATCCGTCTTTCCTCCT+TGG | 0.333569 | 6_1:+34345405 | None:intergenic |
| GTACAATGGTGGTGCACATT+TGG | 0.354447 | 6_1:+34345473 | None:intergenic |
| GTGTACTCTTGGGTAGAAAA+AGG | 0.362117 | 6_1:+34345574 | None:intergenic |
| GGACATTTGGCTCCGTTTAG+AGG | 0.375402 | 6_1:+34345149 | None:intergenic |
| GGAGCCAAATGTCCCACTAT+TGG | 0.387751 | 6_1:-34345140 | Msa0883360:CDS |
| AGGCCAAGTGCCTTGTTCAT+AGG | 0.407251 | 6_1:+34345594 | None:intergenic |
| TTGTCCAATAGTGGGACATT+TGG | 0.415604 | 6_1:+34345136 | None:intergenic |
| TACCCAAGAGTACACCTAAC+TGG | 0.429425 | 6_1:-34345566 | Msa0883360:CDS |
| TGACCAGTTAGGTGTACTCT+TGG | 0.434583 | 6_1:+34345563 | None:intergenic |
| TGGGGTATATTGTCCAATAG+TGG | 0.449512 | 6_1:+34345127 | None:intergenic |
| CTCCGTTTAGAGGTGAAATC+CGG | 0.472012 | 6_1:+34345159 | None:intergenic |
| AATCCGTCTTTCCTCCTTGG+TGG | 0.472043 | 6_1:+34345408 | None:intergenic |
| GACCAGTTAGGTGTACTCTT+GGG | 0.480674 | 6_1:+34345564 | None:intergenic |
| GGTGGGGTATTGCCCAATGG+TGG | 0.482387 | 6_1:+34345107 | None:intergenic |
| ATCCGGATTTCACCTCTAAA+CGG | 0.486816 | 6_1:-34345161 | Msa0883360:CDS |
| CACCTAACTGGTCAAAGCTC+TGG | 0.493259 | 6_1:-34345554 | Msa0883360:CDS |
| TTGGGCAATACCCCACCATT+GGG | 0.499746 | 6_1:-34345101 | Msa0883360:CDS |
| GGACAATATACCCCACCATT+GGG | 0.500116 | 6_1:-34345119 | Msa0883360:CDS |
| ATATGCAAATGCTCATGATC+CGG | 0.505122 | 6_1:-34345178 | Msa0883360:CDS |
| CAATGGTGGTGCACATTTGG+AGG | 0.509649 | 6_1:+34345476 | None:intergenic |
| ATTGGGCAATACCCCACCAT+TGG | 0.512359 | 6_1:-34345102 | Msa0883360:CDS |
| TGGACAATATACCCCACCAT+TGG | 0.516563 | 6_1:-34345120 | Msa0883360:CDS |
| ATCCAGAGCTTTGACCAGTT+AGG | 0.527272 | 6_1:+34345552 | None:intergenic |
| GTGGGGTATTGCCCAATGGT+GGG | 0.528545 | 6_1:+34345108 | None:intergenic |
| AGGAGTTATTACCCAATGGT+GGG | 0.534167 | 6_1:+34345090 | None:intergenic |
| TAGGAGTTATTACCCAATGG+TGG | 0.534991 | 6_1:+34345089 | None:intergenic |
| TTTCCTATGAACAAGGCACT+TGG | 0.548981 | 6_1:-34345597 | Msa0883360:exon |
| GGGGTATATTGTCCAATAGT+GGG | 0.560249 | 6_1:+34345128 | None:intergenic |
| GGAGTTATTACCCAATGGTG+GGG | 0.575053 | 6_1:+34345091 | None:intergenic |
| AACAAGGCTAATACCAGTGT+CGG | 0.575550 | 6_1:-34345510 | Msa0883360:CDS |
| TGGGGTATTGCCCAATGGTG+GGG | 0.583347 | 6_1:+34345109 | None:intergenic |
| CCTCCTTGGTGGTGACGCCG+AGG | 0.608392 | 6_1:+34345419 | None:intergenic |
| AATGGTGGGGTATTGCCCAA+TGG | 0.615658 | 6_1:+34345104 | None:intergenic |
| GGCTTGATATATTCCGACAC+TGG | 0.616970 | 6_1:+34345497 | None:intergenic |
| CCTTGGTGGTGACGCCGAGG+AGG | 0.637667 | 6_1:+34345422 | None:intergenic |
| TCACCACCAAGGAGGAAAGA+CGG | 0.644508 | 6_1:-34345411 | Msa0883360:CDS |
| CCTCCTCGGCGTCACCACCA+AGG | 0.672490 | 6_1:-34345422 | Msa0883360:CDS |
| TGAAGACGCAAGTACAATGG+TGG | 0.682523 | 6_1:+34345462 | None:intergenic |
| CCTCGGCGTCACCACCAAGG+AGG | 0.711600 | 6_1:-34345419 | Msa0883360:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTATTTGGTTATTTT+TGG | + | chr6_1:34345436-34345455 | None:intergenic | 10.0% |
| !!! | TATAAATTTTCCTATGAACA+AGG | - | chr6_1:34345125-34345144 | Msa0883360:CDS | 20.0% |
| !! | GTACGGACTATTTATTTATT+TGG | + | chr6_1:34345446-34345465 | None:intergenic | 25.0% |
| ! | TGTGAAAAGTTTTCTCAACA+AGG | - | chr6_1:34345203-34345222 | Msa0883360:intron | 30.0% |
| ! | TTTGTGCTATGTTTGTTAGT+TGG | + | chr6_1:34345379-34345398 | None:intergenic | 30.0% |
| ! | TTTAGTCAGGTTGTAACTTA+AGG | + | chr6_1:34345506-34345525 | None:intergenic | 30.0% |
| ! | ACTAGATCATTTTACCTCCT+CGG | - | chr6_1:34345293-34345312 | Msa0883360:intron | 35.0% |
| ACTGAGTAATGTGTCATGTA+CGG | + | chr6_1:34345463-34345482 | None:intergenic | 35.0% | |
| CTAAAATGCGTTATCCCTAT+TGG | - | chr6_1:34345521-34345540 | Msa0883360:CDS | 35.0% | |
| TGCATATAGACTGACCAATA+GGG | + | chr6_1:34345538-34345557 | None:intergenic | 35.0% | |
| TTGCATATAGACTGACCAAT+AGG | + | chr6_1:34345539-34345558 | None:intergenic | 35.0% | |
| ATATGCAAATGCTCATGATC+CGG | - | chr6_1:34345551-34345570 | Msa0883360:CDS | 35.0% | |
| ! | TTTCCTATGAACAAGGCACT+TGG | - | chr6_1:34345132-34345151 | Msa0883360:CDS | 40.0% |
| GTGTACTCTTGGGTAGAAAA+AGG | + | chr6_1:34345158-34345177 | None:intergenic | 40.0% | |
| AACAAGGCTAATACCAGTGT+CGG | - | chr6_1:34345219-34345238 | Msa0883360:intron | 40.0% | |
| AGATGAAGACGCAAGTACAA+TGG | + | chr6_1:34345273-34345292 | None:intergenic | 40.0% | |
| ACGTCATCTGTCCAAAAGTA+AGG | - | chr6_1:34345346-34345365 | Msa0883360:intron | 40.0% | |
| CGTCATCTGTCCAAAAGTAA+GGG | - | chr6_1:34345347-34345366 | Msa0883360:intron | 40.0% | |
| ! | TTGGAGATGACCCTTACTTT+TGG | + | chr6_1:34345360-34345379 | None:intergenic | 40.0% |
| !! | AGGGATAACGCATTTTAGTC+AGG | + | chr6_1:34345519-34345538 | None:intergenic | 40.0% |
| ATCCGGATTTCACCTCTAAA+CGG | - | chr6_1:34345568-34345587 | Msa0883360:CDS | 40.0% | |
| ! | TTGTCCAATAGTGGGACATT+TGG | + | chr6_1:34345596-34345615 | None:intergenic | 40.0% |
| GGGGTATATTGTCCAATAGT+GGG | + | chr6_1:34345604-34345623 | None:intergenic | 40.0% | |
| TGGGGTATATTGTCCAATAG+TGG | + | chr6_1:34345605-34345624 | None:intergenic | 40.0% | |
| ! | TACCCAAGAGTACACCTAAC+TGG | - | chr6_1:34345163-34345182 | Msa0883360:CDS | 45.0% |
| GACCAGTTAGGTGTACTCTT+GGG | + | chr6_1:34345168-34345187 | None:intergenic | 45.0% | |
| TGACCAGTTAGGTGTACTCT+TGG | + | chr6_1:34345169-34345188 | None:intergenic | 45.0% | |
| ! | ATCCAGAGCTTTGACCAGTT+AGG | + | chr6_1:34345180-34345199 | None:intergenic | 45.0% |
| GGCTTGATATATTCCGACAC+TGG | + | chr6_1:34345235-34345254 | None:intergenic | 45.0% | |
| ! | GTACAATGGTGGTGCACATT+TGG | + | chr6_1:34345259-34345278 | None:intergenic | 45.0% |
| TGAAGACGCAAGTACAATGG+TGG | + | chr6_1:34345270-34345289 | None:intergenic | 45.0% | |
| TTCAATCCGTCTTTCCTCCT+TGG | + | chr6_1:34345327-34345346 | None:intergenic | 45.0% | |
| TTGTAACTTAAGGCGAGGTG+TGG | + | chr6_1:34345496-34345515 | None:intergenic | 45.0% | |
| ! | TCAGGTTGTAACTTAAGGCG+AGG | + | chr6_1:34345501-34345520 | None:intergenic | 45.0% |
| CTCCGTTTAGAGGTGAAATC+CGG | + | chr6_1:34345573-34345592 | None:intergenic | 45.0% | |
| TGGACAATATACCCCACCAT+TGG | - | chr6_1:34345609-34345628 | Msa0883360:exon | 45.0% | |
| GGACAATATACCCCACCATT+GGG | - | chr6_1:34345610-34345629 | Msa0883360:exon | 45.0% | |
| GGAGTTATTACCCAATGGTG+GGG | + | chr6_1:34345641-34345660 | None:intergenic | 45.0% | |
| ! | AGGCCAAGTGCCTTGTTCAT+AGG | + | chr6_1:34345138-34345157 | None:intergenic | 50.0% |
| CACCTAACTGGTCAAAGCTC+TGG | - | chr6_1:34345175-34345194 | Msa0883360:CDS | 50.0% | |
| ! | CAATGGTGGTGCACATTTGG+AGG | + | chr6_1:34345256-34345275 | None:intergenic | 50.0% |
| TCACCACCAAGGAGGAAAGA+CGG | - | chr6_1:34345318-34345337 | Msa0883360:intron | 50.0% | |
| AATCCGTCTTTCCTCCTTGG+TGG | + | chr6_1:34345324-34345343 | None:intergenic | 50.0% | |
| ! | GGACATTTGGCTCCGTTTAG+AGG | + | chr6_1:34345583-34345602 | None:intergenic | 50.0% |
| GGAGCCAAATGTCCCACTAT+TGG | - | chr6_1:34345589-34345608 | Msa0883360:CDS | 50.0% | |
| !! | AATGGTGGGGTATTGCCCAA+TGG | + | chr6_1:34345628-34345647 | None:intergenic | 50.0% |
| ATTGGGCAATACCCCACCAT+TGG | - | chr6_1:34345627-34345646 | Msa0883360:five_prime_UTR | 50.0% | |
| TTGGGCAATACCCCACCATT+GGG | - | chr6_1:34345628-34345647 | Msa0883360:five_prime_UTR | 50.0% | |
| !! | TGGGGTATTGCCCAATGGTG+GGG | + | chr6_1:34345623-34345642 | None:intergenic | 55.0% |
| !! | GTGGGGTATTGCCCAATGGT+GGG | + | chr6_1:34345624-34345643 | None:intergenic | 55.0% |
| !! | GGTGGGGTATTGCCCAATGG+TGG | + | chr6_1:34345625-34345644 | None:intergenic | 60.0% |
| !! | CCTTGGTGGTGACGCCGAGG+AGG | + | chr6_1:34345310-34345329 | None:intergenic | 70.0% |
| CCTCCTCGGCGTCACCACCA+AGG | - | chr6_1:34345307-34345326 | Msa0883360:intron | 70.0% | |
| !! | CCTCCTTGGTGGTGACGCCG+AGG | + | chr6_1:34345313-34345332 | None:intergenic | 70.0% |
| CCTCGGCGTCACCACCAAGG+AGG | - | chr6_1:34345310-34345329 | Msa0883360:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 34345091 | 34345660 | 34345091 | ID=Msa0883360;Name=Msa0883360 |
| chr6_1 | mRNA | 34345091 | 34345660 | 34345091 | ID=Msa0883360-mRNA-1;Parent=Msa0883360;Name=Msa0883360-mRNA-1;_AED=0.62;_eAED=0.62;_QI=47|0|0|1|0|0|3|0|105 |
| chr6_1 | exon | 34345477 | 34345660 | 34345477 | ID=Msa0883360-mRNA-1:exon:8357;Parent=Msa0883360-mRNA-1 |
| chr6_1 | exon | 34345389 | 34345453 | 34345389 | ID=Msa0883360-mRNA-1:exon:8356;Parent=Msa0883360-mRNA-1 |
| chr6_1 | exon | 34345091 | 34345203 | 34345091 | ID=Msa0883360-mRNA-1:exon:8355;Parent=Msa0883360-mRNA-1 |
| chr6_1 | five_prime_UTR | 34345614 | 34345660 | 34345614 | ID=Msa0883360-mRNA-1:five_prime_utr;Parent=Msa0883360-mRNA-1 |
| chr6_1 | CDS | 34345477 | 34345613 | 34345477 | ID=Msa0883360-mRNA-1:cds;Parent=Msa0883360-mRNA-1 |
| chr6_1 | CDS | 34345389 | 34345453 | 34345389 | ID=Msa0883360-mRNA-1:cds;Parent=Msa0883360-mRNA-1 |
| chr6_1 | CDS | 34345091 | 34345203 | 34345091 | ID=Msa0883360-mRNA-1:cds;Parent=Msa0883360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
