AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1092990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1092990 MtrunA17_Chr2g0308161 72.131 61 12 1 60 115 19 79 1.54e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACCATTTCTTGAAGTT+GGG 0.352117 7_3:+11785139 None:intergenic
TTTCAACCATTTCTTGAAGT+TGG 0.356872 7_3:+11785138 None:intergenic
CTAGTCCCAACTTCAAGAAA+TGG 0.372385 7_3:-11785144 Msa1092990:intron
AAGCAATTGGATGTAGAAAC+TGG 0.389575 7_3:-11785272 Msa1092990:CDS
ATGGGAAAGGAGTGTGTACC+AGG 0.471236 7_3:-11785080 Msa1092990:intron
CTTGAGGACAACATAGGTGA+TGG 0.475620 7_3:-11784631 Msa1092990:CDS
ACTTCAAGAAATGGTTGAAA+AGG 0.483448 7_3:-11785135 Msa1092990:CDS
AGGAAGTAGAGGAAAGCAAT+TGG 0.498254 7_3:-11785285 Msa1092990:CDS
ATGAAATTCAAGAGGATGAT+CGG 0.537624 7_3:-11784558 Msa1092990:CDS
GAATTTCTTGAGGACAACAT+AGG 0.539954 7_3:-11784637 Msa1092990:CDS
TCAAGAAATGGTTGAAAAGG+AGG 0.541538 7_3:-11785132 Msa1092990:CDS
TTGAGGACAACATAGGTGAT+GGG 0.545907 7_3:-11784630 Msa1092990:CDS
CGCATATCATGAATTTCTTG+AGG 0.553600 7_3:-11784647 Msa1092990:CDS
GAAACTCGACAAGGAAGTAG+AGG 0.581711 7_3:-11785296 Msa1092990:CDS
TATATATGATGAATTTCAAG+AGG 0.613332 7_3:-11784593 Msa1092990:CDS
TCTAGATGATGAAATTCAAG+AGG 0.613572 7_3:-11784566 Msa1092990:CDS
CATAGGTGATGGGTTTCAGA+AGG 0.615780 7_3:-11784620 Msa1092990:CDS
CATAGATGAGGAATTTCAAG+AGG 0.630357 7_3:-11784674 Msa1092990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGTAAAAATTTAAAGTTA+CGG - chr7_3:11784622-11784641 Msa1092990:CDS 10.0%
!!! TATGTAAAAATTTAAAGTTA+CGG - chr7_3:11784622-11784641 Msa1092990:CDS 10.0%
!! AAATTGTGTTTAACTAATAT+AGG - chr7_3:11784646-11784665 Msa1092990:CDS 15.0%
!! AAATTGTGTTTAACTAATAT+AGG - chr7_3:11784646-11784665 Msa1092990:CDS 15.0%
!! AAGTTGAAGAAACTTAAAAA+TGG - chr7_3:11784900-11784919 Msa1092990:intron 20.0%
!! AGTTGAAGAAACTTAAAAAT+GGG - chr7_3:11784901-11784920 Msa1092990:intron 20.0%
!!! TTTTATTTTCATATGCAACA+AGG - chr7_3:11785094-11785113 Msa1092990:CDS 20.0%
!!! AATTATTTTCATATGCAACA+AGG - chr7_3:11785136-11785155 Msa1092990:CDS 20.0%
!! TATATATGATGAATTTCAAG+AGG - chr7_3:11785267-11785286 Msa1092990:intron 20.0%
!! AAGTTGAAGAAACTTAAAAA+TGG - chr7_3:11784900-11784919 Msa1092990:intron 20.0%
!! AGTTGAAGAAACTTAAAAAT+GGG - chr7_3:11784901-11784920 Msa1092990:intron 20.0%
!!! TTTTATTTTCATATGCAACA+AGG - chr7_3:11785094-11785113 Msa1092990:CDS 20.0%
!!! AATTATTTTCATATGCAACA+AGG - chr7_3:11785136-11785155 Msa1092990:CDS 20.0%
!! TATATATGATGAATTTCAAG+AGG - chr7_3:11785267-11785286 Msa1092990:intron 20.0%
! TTGTGTTTAACTAATATAGG+CGG - chr7_3:11784649-11784668 Msa1092990:CDS 25.0%
! TTGTAATGATAGAATTAGGA+AGG - chr7_3:11784925-11784944 Msa1092990:intron 25.0%
! TGTAATGATAGAATTAGGAA+GGG - chr7_3:11784926-11784945 Msa1092990:intron 25.0%
! CTTTGAATCAACAATATCTA+CGG - chr7_3:11785058-11785077 Msa1092990:intron 25.0%
! TTGTGTTTAACTAATATAGG+CGG - chr7_3:11784649-11784668 Msa1092990:CDS 25.0%
! TTGTAATGATAGAATTAGGA+AGG - chr7_3:11784925-11784944 Msa1092990:intron 25.0%
! TGTAATGATAGAATTAGGAA+GGG - chr7_3:11784926-11784945 Msa1092990:intron 25.0%
! CTTTGAATCAACAATATCTA+CGG - chr7_3:11785058-11785077 Msa1092990:intron 25.0%
!!! ATGTTTTTTGAAACTCGACA+AGG - chr7_3:11784555-11784574 Msa1092990:CDS 30.0%
TTCAACCATTTCTTGAAGTT+GGG + chr7_3:11784724-11784743 None:intergenic 30.0%
TTTCAACCATTTCTTGAAGT+TGG + chr7_3:11784725-11784744 None:intergenic 30.0%
ACTTCAAGAAATGGTTGAAA+AGG - chr7_3:11784725-11784744 Msa1092990:CDS 30.0%
!!! GAAGCTTTTAAAGCTGTTAT+GGG - chr7_3:11784762-11784781 Msa1092990:intron 30.0%
!!! TTTTAAAGCTGTTATGGGAA+AGG - chr7_3:11784767-11784786 Msa1092990:intron 30.0%
CAAAACAAATGTTCTACTTG+CGG - chr7_3:11784956-11784975 Msa1092990:intron 30.0%
!!! ATTTGGTTTTTATTTGTGCG+AGG - chr7_3:11785007-11785026 Msa1092990:intron 30.0%
!! CGTAGATATTGTTGATTCAA+AGG + chr7_3:11785060-11785079 None:intergenic 30.0%
TGAAAAAGATGACATAGATG+AGG - chr7_3:11785174-11785193 Msa1092990:intron 30.0%
TCTAGATGATGAAATTCAAG+AGG - chr7_3:11785294-11785313 Msa1092990:CDS 30.0%
ATGAAATTCAAGAGGATGAT+CGG - chr7_3:11785302-11785321 Msa1092990:CDS 30.0%
!!! ATGTTTTTTGAAACTCGACA+AGG - chr7_3:11784555-11784574 Msa1092990:CDS 30.0%
TTCAACCATTTCTTGAAGTT+GGG + chr7_3:11784724-11784743 None:intergenic 30.0%
TTTCAACCATTTCTTGAAGT+TGG + chr7_3:11784725-11784744 None:intergenic 30.0%
ACTTCAAGAAATGGTTGAAA+AGG - chr7_3:11784725-11784744 Msa1092990:CDS 30.0%
!!! GAAGCTTTTAAAGCTGTTAT+GGG - chr7_3:11784762-11784781 Msa1092990:intron 30.0%
!!! TTTTAAAGCTGTTATGGGAA+AGG - chr7_3:11784767-11784786 Msa1092990:intron 30.0%
CAAAACAAATGTTCTACTTG+CGG - chr7_3:11784956-11784975 Msa1092990:intron 30.0%
!!! ATTTGGTTTTTATTTGTGCG+AGG - chr7_3:11785007-11785026 Msa1092990:intron 30.0%
!! CGTAGATATTGTTGATTCAA+AGG + chr7_3:11785060-11785079 None:intergenic 30.0%
TGAAAAAGATGACATAGATG+AGG - chr7_3:11785174-11785193 Msa1092990:intron 30.0%
TCTAGATGATGAAATTCAAG+AGG - chr7_3:11785294-11785313 Msa1092990:CDS 30.0%
ATGAAATTCAAGAGGATGAT+CGG - chr7_3:11785302-11785321 Msa1092990:CDS 30.0%
AAGCAATTGGATGTAGAAAC+TGG - chr7_3:11784588-11784607 Msa1092990:CDS 35.0%
TCAAGAAATGGTTGAAAAGG+AGG - chr7_3:11784728-11784747 Msa1092990:CDS 35.0%
!!! CGAAGCTTTTAAAGCTGTTA+TGG - chr7_3:11784761-11784780 Msa1092990:intron 35.0%
GGGCTTGTAATGATAGAATT+AGG - chr7_3:11784921-11784940 Msa1092990:intron 35.0%
!! GCGTGTTTTACTTGTTGATT+TGG - chr7_3:11784990-11785009 Msa1092990:intron 35.0%
CATAGATGAGGAATTTCAAG+AGG - chr7_3:11785186-11785205 Msa1092990:intron 35.0%
CGCATATCATGAATTTCTTG+AGG - chr7_3:11785213-11785232 Msa1092990:intron 35.0%
GAATTTCTTGAGGACAACAT+AGG - chr7_3:11785223-11785242 Msa1092990:intron 35.0%
AAGCAATTGGATGTAGAAAC+TGG - chr7_3:11784588-11784607 Msa1092990:CDS 35.0%
TCAAGAAATGGTTGAAAAGG+AGG - chr7_3:11784728-11784747 Msa1092990:CDS 35.0%
!!! CGAAGCTTTTAAAGCTGTTA+TGG - chr7_3:11784761-11784780 Msa1092990:intron 35.0%
GGGCTTGTAATGATAGAATT+AGG - chr7_3:11784921-11784940 Msa1092990:intron 35.0%
!! GCGTGTTTTACTTGTTGATT+TGG - chr7_3:11784990-11785009 Msa1092990:intron 35.0%
CATAGATGAGGAATTTCAAG+AGG - chr7_3:11785186-11785205 Msa1092990:intron 35.0%
CGCATATCATGAATTTCTTG+AGG - chr7_3:11785213-11785232 Msa1092990:intron 35.0%
GAATTTCTTGAGGACAACAT+AGG - chr7_3:11785223-11785242 Msa1092990:intron 35.0%
AGGAAGTAGAGGAAAGCAAT+TGG - chr7_3:11784575-11784594 Msa1092990:CDS 40.0%
CTAGTCCCAACTTCAAGAAA+TGG - chr7_3:11784716-11784735 Msa1092990:CDS 40.0%
AACACAATCAAAATCCGACC+TGG + chr7_3:11784801-11784820 None:intergenic 40.0%
TTGAGGACAACATAGGTGAT+GGG - chr7_3:11785230-11785249 Msa1092990:intron 40.0%
AGGAAGTAGAGGAAAGCAAT+TGG - chr7_3:11784575-11784594 Msa1092990:CDS 40.0%
CTAGTCCCAACTTCAAGAAA+TGG - chr7_3:11784716-11784735 Msa1092990:CDS 40.0%
AACACAATCAAAATCCGACC+TGG + chr7_3:11784801-11784820 None:intergenic 40.0%
TTGAGGACAACATAGGTGAT+GGG - chr7_3:11785230-11785249 Msa1092990:intron 40.0%
GAAACTCGACAAGGAAGTAG+AGG - chr7_3:11784564-11784583 Msa1092990:CDS 45.0%
CTTGAGGACAACATAGGTGA+TGG - chr7_3:11785229-11785248 Msa1092990:intron 45.0%
!! CATAGGTGATGGGTTTCAGA+AGG - chr7_3:11785240-11785259 Msa1092990:intron 45.0%
GAAACTCGACAAGGAAGTAG+AGG - chr7_3:11784564-11784583 Msa1092990:CDS 45.0%
CTTGAGGACAACATAGGTGA+TGG - chr7_3:11785229-11785248 Msa1092990:intron 45.0%
!! CATAGGTGATGGGTTTCAGA+AGG - chr7_3:11785240-11785259 Msa1092990:intron 45.0%
! ATGGGAAAGGAGTGTGTACC+AGG - chr7_3:11784780-11784799 Msa1092990:intron 50.0%
! GAAAGGAGTGTGTACCAGGT+CGG - chr7_3:11784784-11784803 Msa1092990:intron 50.0%
! ATGGGAAAGGAGTGTGTACC+AGG - chr7_3:11784780-11784799 Msa1092990:intron 50.0%
! GAAAGGAGTGTGTACCAGGT+CGG - chr7_3:11784784-11784803 Msa1092990:intron 50.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 11784555 11785327 11784555 ID=Msa1092990;Name=Msa1092990
chr7_3 mRNA 11784555 11785327 11784555 ID=Msa1092990-mRNA-1;Parent=Msa1092990;Name=Msa1092990-mRNA-1;_AED=0.40;_eAED=0.41;_QI=0|0|0|1|1|1|3|0|117
chr7_3 exon 11785268 11785327 11785268 ID=Msa1092990-mRNA-1:exon:2885;Parent=Msa1092990-mRNA-1
chr7_3 exon 11785081 11785162 11785081 ID=Msa1092990-mRNA-1:exon:2884;Parent=Msa1092990-mRNA-1
chr7_3 exon 11784555 11784766 11784555 ID=Msa1092990-mRNA-1:exon:2883;Parent=Msa1092990-mRNA-1
chr7_3 CDS 11785268 11785327 11785268 ID=Msa1092990-mRNA-1:cds;Parent=Msa1092990-mRNA-1
chr7_3 CDS 11785081 11785162 11785081 ID=Msa1092990-mRNA-1:cds;Parent=Msa1092990-mRNA-1
chr7_3 CDS 11784555 11784766 11784555 ID=Msa1092990-mRNA-1:cds;Parent=Msa1092990-mRNA-1
Gene Sequence

>Msa1092990

ATGTTTTTTGAAACTCGACAAGGAAGTAGAGGAAAGCAATTGGATGTAGAAACTGGAAAATCCCAACTTCAAGAAATGGTTGAAAAGGAGGAAAGTGATATCGAAGCTTTTAAAGCTGTTATGGGAAAGGAGTGTGTACCAGGTATCGATGAAGATGTTGAAAATTATTTTCATATGCAACAAGGTATCGATGAAGATGTTGAAAAAGATGACATAGATGAGGAATTTCAAGAGGAAGACGCATATCATGAATTTCTTGAGGACAACATAGGTGATGGGTTTCAGAAGGATGATATATATGATGAATTTCAAGAGGATGATCTAGATGATGAAATTCAAGAGGATGATCGGTGA

Protein sequence

>Msa1092990

MFFETRQGSRGKQLDVETGKSQLQEMVEKEESDIEAFKAVMGKECVPGIDEDVENYFHMQQGIDEDVEKDDIDEEFQEEDAYHEFLEDNIGDGFQKDDIYDEFQEDDLDDEIQEDDR*