Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0211680 | KEH17167.1 | 90.625 | 96 | 9 | 0 | 1 | 96 | 1 | 96 | 6.10e-35 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0211680 | A0A072TID1 | 90.625 | 96 | 9 | 0 | 1 | 96 | 1 | 96 | 2.91e-35 | 125 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0023610 | Msa0211680 | 0.876525 | 1.302552e-68 | -8.615850e-47 |
| Msa0068630 | Msa0211680 | 0.841279 | 5.231369e-58 | -8.615850e-47 |
| Msa0071340 | Msa0211680 | 0.865998 | 3.931021e-65 | -8.615850e-47 |
| Msa0153200 | Msa0211680 | 0.895517 | 8.894857e-76 | -8.615850e-47 |
| Msa0162580 | Msa0211680 | 0.819800 | 9.601894e-53 | -8.615850e-47 |
| Msa0211680 | Msa0235190 | 0.815338 | 9.729539e-52 | -8.615850e-47 |
| Msa0211680 | Msa0273570 | 0.835685 | 1.452878e-56 | -8.615850e-47 |
| Msa0211680 | Msa0280450 | 0.824056 | 9.924469e-54 | -8.615850e-47 |
| Msa0211680 | Msa0291600 | 0.908643 | 1.365370e-81 | -8.615850e-47 |
| Msa0211680 | Msa0343610 | 0.806011 | 1.011890e-49 | -8.615850e-47 |
| Msa0211680 | Msa0365810 | 0.813738 | 2.199001e-51 | -8.615850e-47 |
| Msa0211680 | Msa0414590 | 0.814856 | 1.245178e-51 | -8.615850e-47 |
| Msa0211680 | Msa0458700 | 0.820458 | 6.786500e-53 | -8.615850e-47 |
| Msa0211680 | Msa0533290 | 0.804001 | 2.663400e-49 | -8.615850e-47 |
| Msa0211680 | Msa0533430 | 0.844894 | 5.699325e-59 | -8.615850e-47 |
| Msa0211680 | Msa0580610 | 0.861320 | 1.114178e-63 | -8.615850e-47 |
| Msa0211680 | Msa0629940 | 0.806834 | 6.785893e-50 | -8.615850e-47 |
| Msa0211680 | Msa0675510 | 0.845405 | 4.146397e-59 | -8.615850e-47 |
| Msa0211680 | Msa0754180 | 0.828743 | 7.587458e-55 | -8.615850e-47 |
| Msa0211680 | Msa0784520 | 0.819133 | 1.362977e-52 | -8.615850e-47 |
| Msa0211680 | Msa0815320 | 0.815207 | 1.040609e-51 | -8.615850e-47 |
| Msa0211680 | Msa0818010 | 0.872838 | 2.338544e-67 | -8.615850e-47 |
| Msa0211680 | Msa0818030 | 0.860405 | 2.112888e-63 | -8.615850e-47 |
| Msa0211680 | Msa0858360 | 0.876704 | 1.130127e-68 | -8.615850e-47 |
| Msa0211680 | Msa0883360 | 0.803368 | 3.605089e-49 | -8.615850e-47 |
| Msa0211680 | Msa0883370 | 0.827402 | 1.595463e-54 | -8.615850e-47 |
| Msa0211680 | Msa0884920 | 0.854186 | 1.455908e-61 | -8.615850e-47 |
| Msa0211680 | Msa0898390 | 0.800577 | 1.350519e-48 | -8.615850e-47 |
| Msa0211680 | Msa0902020 | 0.809777 | 1.599652e-50 | -8.615850e-47 |
| Msa0211680 | Msa0912370 | 0.806827 | 6.807598e-50 | -8.615850e-47 |
| Msa0211680 | Msa0912500 | 0.858359 | 8.694131e-63 | -8.615850e-47 |
| Msa0211680 | Msa0924370 | 0.809556 | 1.785269e-50 | -8.615850e-47 |
| Msa0211680 | Msa0963310 | 0.818684 | 1.723459e-52 | -8.615850e-47 |
| Msa0211680 | Msa1006250 | 0.880618 | 4.731981e-70 | -8.615850e-47 |
| Msa0211680 | Msa1027240 | 0.836517 | 8.932123e-57 | -8.615850e-47 |
| Msa0211680 | Msa1048490 | 0.811985 | 5.323877e-51 | -8.615850e-47 |
| Msa0211680 | Msa1048610 | 0.803185 | 3.933148e-49 | -8.615850e-47 |
| Msa0211680 | Msa1100180 | 0.822460 | 2.340897e-53 | -8.615850e-47 |
| Msa0211680 | Msa1131820 | 0.809828 | 1.560077e-50 | -8.615850e-47 |
| Msa0211680 | Msa1178650 | 0.812186 | 4.812442e-51 | -8.615850e-47 |
| Msa0211680 | Msa1178660 | 0.843417 | 1.419324e-58 | -8.615850e-47 |
| Msa0211680 | Msa1182730 | 0.819258 | 1.276723e-52 | -8.615850e-47 |
| Msa0211680 | Msa1187060 | 0.830257 | 3.251390e-55 | -8.615850e-47 |
| Msa0211680 | Msa1196030 | 0.807610 | 4.647918e-50 | -8.615850e-47 |
| Msa0211680 | Msa1287560 | 0.804073 | 2.572663e-49 | -8.615850e-47 |
| Msa0211680 | Msa1287700 | 0.804727 | 1.880087e-49 | -8.615850e-47 |
| Msa0211680 | Msa1288000 | 0.839877 | 1.218094e-57 | -8.615850e-47 |
| Msa0211680 | Msa1347360 | 0.820605 | 6.278630e-53 | -8.615850e-47 |
| Msa0211680 | Msa1359820 | 0.857585 | 1.476767e-62 | -8.615850e-47 |
| Msa0211680 | Msa1406600 | 0.800949 | 1.133873e-48 | -8.615850e-47 |
| Msa0211680 | Msa1423990 | 0.864247 | 1.395293e-64 | -8.615850e-47 |
| Msa0211680 | Msa1444370 | 0.850514 | 1.616838e-60 | -8.615850e-47 |
| Msa0211680 | Msa1463140 | 0.878524 | 2.620135e-69 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATAAGTGTTAAACTTCT+TGG | 0.164387 | 2_1:-53216452 | None:intergenic |
| TTTCTTACCAGCCATAGCTT+TGG | 0.395295 | 2_1:+53216625 | Msa0211680:CDS |
| GAACGAAGCACAAAAGGATA+TGG | 0.440435 | 2_1:+53216420 | Msa0211680:CDS |
| TGTTCTGTTGACATCAGTTA+AGG | 0.466515 | 2_1:-53216648 | None:intergenic |
| GATGTCAACAGAACAGTTGT+TGG | 0.472083 | 2_1:+53216656 | Msa0211680:CDS |
| AAGCACAAAAGGATATGGTT+TGG | 0.477101 | 2_1:+53216425 | Msa0211680:CDS |
| AATCGTGAACGAAGCACAAA+AGG | 0.486280 | 2_1:+53216414 | Msa0211680:CDS |
| GAGCACTTCCTCAACAAGTT+GGG | 0.488474 | 2_1:+53216571 | Msa0211680:CDS |
| CACTTATGAAGATGCTCTCA+TGG | 0.494764 | 2_1:+53216465 | Msa0211680:CDS |
| GGAGCACTTCCTCAACAAGT+TGG | 0.533066 | 2_1:+53216570 | Msa0211680:CDS |
| ATCAGTTAAGGCCAAAGCTA+TGG | 0.533572 | 2_1:-53216636 | None:intergenic |
| AAGTTTCACAGTTGAAGTAG+TGG | 0.566854 | 2_1:+53216679 | Msa0211680:CDS |
| TTTCACAGTTGAAGTAGTGG+TGG | 0.573034 | 2_1:+53216682 | Msa0211680:CDS |
| GTTAAGGCCAAAGCTATGGC+TGG | 0.581908 | 2_1:-53216632 | None:intergenic |
| CTTGTTATCCCAACTTGTTG+AGG | 0.598354 | 2_1:-53216579 | None:intergenic |
| AGTAGAAGAATATGATGCCA+TGG | 0.617826 | 2_1:-53216374 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCAAGAATCTTTCTAAAAA+TGG | + | chr2_1:53216511-53216530 | Msa0211680:intron | 20.0% |
| !!! | TCAAGAATCTTTCTAAAAAT+GGG | + | chr2_1:53216512-53216531 | Msa0211680:intron | 20.0% |
| ! | TTCATAAGTGTTAAACTTCT+TGG | - | chr2_1:53216455-53216474 | None:intergenic | 25.0% |
| ! | TTCACGATTGAAACATTTTC+AGG | - | chr2_1:53216403-53216422 | None:intergenic | 30.0% |
| GTATGATCTTCTATCTACTA+TGG | + | chr2_1:53216534-53216553 | Msa0211680:intron | 30.0% | |
| ! | CTATCTACTATGGTTTTTCA+AGG | + | chr2_1:53216544-53216563 | Msa0211680:intron | 30.0% |
| AAGCACAAAAGGATATGGTT+TGG | + | chr2_1:53216425-53216444 | Msa0211680:CDS | 35.0% | |
| !!! | TACTATGGTTTTTCAAGGCT+AGG | + | chr2_1:53216549-53216568 | Msa0211680:intron | 35.0% |
| TGTTCTGTTGACATCAGTTA+AGG | - | chr2_1:53216651-53216670 | None:intergenic | 35.0% | |
| AAGTTTCACAGTTGAAGTAG+TGG | + | chr2_1:53216679-53216698 | Msa0211680:CDS | 35.0% | |
| AATCGTGAACGAAGCACAAA+AGG | + | chr2_1:53216414-53216433 | Msa0211680:CDS | 40.0% | |
| GAACGAAGCACAAAAGGATA+TGG | + | chr2_1:53216420-53216439 | Msa0211680:CDS | 40.0% | |
| CACTTATGAAGATGCTCTCA+TGG | + | chr2_1:53216465-53216484 | Msa0211680:CDS | 40.0% | |
| !! | CTTGTTATCCCAACTTGTTG+AGG | - | chr2_1:53216582-53216601 | None:intergenic | 40.0% |
| TTTCTTACCAGCCATAGCTT+TGG | + | chr2_1:53216625-53216644 | Msa0211680:CDS | 40.0% | |
| ATCAGTTAAGGCCAAAGCTA+TGG | - | chr2_1:53216639-53216658 | None:intergenic | 40.0% | |
| GATGTCAACAGAACAGTTGT+TGG | + | chr2_1:53216656-53216675 | Msa0211680:CDS | 40.0% | |
| TTTCACAGTTGAAGTAGTGG+TGG | + | chr2_1:53216682-53216701 | Msa0211680:CDS | 40.0% | |
| GAGCACTTCCTCAACAAGTT+GGG | + | chr2_1:53216571-53216590 | Msa0211680:CDS | 45.0% | |
| ! | GGAGCACTTCCTCAACAAGT+TGG | + | chr2_1:53216570-53216589 | Msa0211680:CDS | 50.0% |
| GTTAAGGCCAAAGCTATGGC+TGG | - | chr2_1:53216635-53216654 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 53216376 | 53216751 | 53216376 | ID=Msa0211680;Name=Msa0211680 |
| chr2_1 | mRNA | 53216376 | 53216751 | 53216376 | ID=Msa0211680-mRNA-1;Parent=Msa0211680;Name=Msa0211680-mRNA-1;_AED=0.11;_eAED=0.14;_QI=0|0|0|1|1|1|2|0|98 |
| chr2_1 | exon | 53216376 | 53216486 | 53216376 | ID=Msa0211680-mRNA-1:exon:14799;Parent=Msa0211680-mRNA-1 |
| chr2_1 | exon | 53216566 | 53216751 | 53216566 | ID=Msa0211680-mRNA-1:exon:14800;Parent=Msa0211680-mRNA-1 |
| chr2_1 | CDS | 53216376 | 53216486 | 53216376 | ID=Msa0211680-mRNA-1:cds;Parent=Msa0211680-mRNA-1 |
| chr2_1 | CDS | 53216566 | 53216751 | 53216566 | ID=Msa0211680-mRNA-1:cds;Parent=Msa0211680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
