AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0888170


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0888170 MtrunA17_Chr6g0469571 95.161 124 6 0 1 124 1 124 8.23e-75 218
Msa0888170 MtrunA17_Chr6g0469551 93.548 124 8 0 1 124 1 124 2.97e-74 216
Msa0888170 MtrunA17_Chr6g0469591 93.548 124 8 0 1 124 1 124 1.34e-73 214
Msa0888170 MtrunA17_Chr7g0233231 57.407 108 44 1 1 108 1 106 1.27e-38 125
Msa0888170 MtrunA17_Chr6g0469681 59.690 129 45 3 1 124 1 127 2.69e-38 125
Msa0888170 MtrunA17_Chr7g0233211 57.009 107 44 1 1 107 1 105 2.03e-37 122
Msa0888170 MtrunA17_Chr1g0202541 56.296 135 47 2 1 123 1 135 5.57e-37 122
Msa0888170 MtrunA17_Chr7g0233251 57.018 114 44 2 1 111 1 112 5.87e-37 121
Msa0888170 MtrunA17_Chr1g0202561 52.899 138 50 2 1 123 1 138 1.24e-36 122
Msa0888170 MtrunA17_Chr7g0233191 57.009 107 44 1 1 107 1 105 2.20e-36 120
Msa0888170 MtrunA17_Chr7g0233201 57.009 107 44 1 1 107 1 105 4.89e-36 119
Msa0888170 MtrunA17_Chr6g0469541 59.813 107 41 2 1 107 1 105 9.94e-36 118
Msa0888170 MtrunA17_Chr6g0469651 55.224 134 49 2 1 124 1 133 1.04e-35 119
Msa0888170 MtrunA17_Chr7g0233181 55.660 106 45 1 1 106 1 104 3.77e-35 117
Msa0888170 MtrunA17_Chr1g0202581 55.556 135 44 3 1 123 1 131 3.07e-34 115
Msa0888170 MtrunA17_Chr6g0469671 78.571 70 15 0 1 70 1 70 4.38e-34 114
Msa0888170 MtrunA17_Chr8g0387471 49.618 131 55 3 1 124 1 127 2.96e-33 112
Msa0888170 MtrunA17_Chr1g0202501 50.000 134 53 3 1 124 1 130 7.32e-33 112
Msa0888170 MtrunA17_Chr1g0204291 51.852 135 49 4 1 123 1 131 1.14e-32 111
Msa0888170 MtrunA17_Chr7g0248671 61.765 68 26 0 1 68 1 68 6.35e-24 90.5
Msa0888170 MtrunA17_Chr8g0348361 39.844 128 52 1 22 124 22 149 5.36e-15 67.0
Msa0888170 MtrunA17_Chr3g0120541 44.444 81 41 4 3 79 5 85 6.00e-14 63.2
Msa0888170 MtrunA17_Chr5g0404641 31.373 153 73 4 3 123 4 156 2.04e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0888170 AT1G01490.1 66.197 71 23 1 1 70 1 71 7.12e-25 93.6
Msa0888170 AT1G01490.2 66.197 71 23 1 1 70 1 71 7.12e-25 93.6
Msa0888170 AT1G01490.4 65.714 70 23 1 2 70 29 98 2.49e-24 92.8
Msa0888170 AT5G52740.1 55.714 70 29 1 1 70 1 68 7.66e-19 76.3
Msa0888170 AT5G52760.1 41.176 102 54 3 5 105 7 103 2.21e-15 67.8
Msa0888170 AT5G52750.1 45.455 66 36 0 2 67 4 69 5.14e-13 62.0
Msa0888170 AT1G63950.2 28.000 125 80 2 1 124 1 116 4.57e-11 56.2

Find 32 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACAGTCTCTGGCCTTTC+AGG 0.146342 6_1:+52352487 Msa0888170:CDS
AACCAACAGCTCTGATTTAT+TGG 0.196653 6_1:+52352288 Msa0888170:five_prime_UTR
AGACTTCAAGTAATTGAATT+TGG 0.223914 6_1:-52353037 None:intergenic
TTGAAGATGAAAAGTGTTAT+AGG 0.257481 6_1:-52352225 None:intergenic
GACAAGAAATTAACCTTAAC+TGG 0.272704 6_1:+52352891 Msa0888170:CDS
TGTATTTCTTATACTTTCAA+TGG 0.370006 6_1:-52353307 None:intergenic
ATATTCCTCTGTTTCAAGAA+TGG 0.394512 6_1:+52353100 Msa0888170:CDS
TAAAAGAAGAGCCAGGGTAT+TGG 0.407517 6_1:-52352259 None:intergenic
TGGATCTGTGTCTCCAGTTA+AGG 0.422934 6_1:-52352904 None:intergenic
ATTTGGTCCTTCTTCACATC+CGG 0.427221 6_1:-52353020 None:intergenic
TTAACAGAAACTAGTATTGA+AGG 0.442263 6_1:+52352426 Msa0888170:intron
AGCTTTATTGAAATTAATGA+TGG 0.446473 6_1:+52352320 Msa0888170:exon
AGCTTACTTACTACATGTAC+TGG 0.457206 6_1:-52352924 None:intergenic
TAAAATTTAAAAGAAGAGCC+AGG 0.474794 6_1:-52352266 None:intergenic
ATGCTGAAATAGTTTCTGTT+GGG 0.476513 6_1:+52352961 Msa0888170:CDS
AAAGCTATGAAGACAGTCTC+TGG 0.487579 6_1:+52352478 Msa0888170:CDS
CATGCTGAAATAGTTTCTGT+TGG 0.500770 6_1:+52352960 Msa0888170:CDS
TAGTAAGTAAGCTAAGGAAA+TGG 0.502836 6_1:+52352934 Msa0888170:CDS
CAGCATATGACACATCCAAC+AGG 0.518380 6_1:-52353131 None:intergenic
ATCTAAGCCGGATGTGAAGA+AGG 0.520357 6_1:+52353013 Msa0888170:CDS
GACCAATAAATCAGAGCTGT+TGG 0.529385 6_1:-52352290 None:intergenic
GAGGAATATTGAATATATTG+TGG 0.535752 6_1:-52353086 None:intergenic
TACATGTAGTAAGTAAGCTA+AGG 0.555076 6_1:+52352928 Msa0888170:CDS
AAAATTTAAAAGAAGAGCCA+GGG 0.576568 6_1:-52352265 None:intergenic
TTGTTCCATTCTTGAAACAG+AGG 0.577364 6_1:-52353105 None:intergenic
GAAGAAAGAAGAATCTAAGC+CGG 0.577768 6_1:+52353001 Msa0888170:CDS
ACAGAAACTAGTATTGAAGG+TGG 0.588633 6_1:+52352429 Msa0888170:intron
ATTCTATAGCTCCAATACCC+TGG 0.617469 6_1:+52352248 Msa0888170:five_prime_UTR
ACATGTCGACATTACCTGAA+AGG 0.624790 6_1:-52352501 None:intergenic
AGAATGGAACAAGATCCTGT+TGG 0.628668 6_1:+52353116 Msa0888170:CDS
GTGTTCAATTGTGCAAACAA+AGG 0.635392 6_1:+52353262 Msa0888170:three_prime_UTR
TGTTGCATATGATAATAATG+AGG 0.657518 6_1:-52353062 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTAATAGTAAAAGA+GGG - chr6_1:52352369-52352388 None:intergenic 10.0%
!!! TGTATTTTCATATTATTTAT+TGG + chr6_1:52353179-52353198 Msa0888170:three_prime_UTR 10.0%
!! AAAAAATTAATAGTAAAAGA+GGG - chr6_1:52352369-52352388 None:intergenic 10.0%
!!! TGTATTTTCATATTATTTAT+TGG + chr6_1:52353179-52353198 Msa0888170:three_prime_UTR 10.0%
!! GAAAAAATTAATAGTAAAAG+AGG - chr6_1:52352370-52352389 None:intergenic 15.0%
!!! TTATTTTGCTAAAATTGTAT+TGG + chr6_1:52352825-52352844 Msa0888170:intron 15.0%
!!! ATTTTTATATTGTTTTTGAG+TGG + chr6_1:52353216-52353235 Msa0888170:three_prime_UTR 15.0%
!! GAAAAAATTAATAGTAAAAG+AGG - chr6_1:52352370-52352389 None:intergenic 15.0%
!!! TTATTTTGCTAAAATTGTAT+TGG + chr6_1:52352825-52352844 Msa0888170:intron 15.0%
!!! ATTTTTATATTGTTTTTGAG+TGG + chr6_1:52353216-52353235 Msa0888170:three_prime_UTR 15.0%
!!! AGCTTTATTGAAATTAATGA+TGG + chr6_1:52352320-52352339 Msa0888170:exon 20.0%
!! TTCACATTATTTAATCTTGA+GGG + chr6_1:52352630-52352649 Msa0888170:intron 20.0%
!! TCAAATTATGACGTAAAAAA+GGG - chr6_1:52352672-52352691 None:intergenic 20.0%
!! TTCAAATTATGACGTAAAAA+AGG - chr6_1:52352673-52352692 None:intergenic 20.0%
!! TGTATTTCTTATACTTTCAA+TGG - chr6_1:52353310-52353329 None:intergenic 20.0%
!!! AGCTTTATTGAAATTAATGA+TGG + chr6_1:52352320-52352339 Msa0888170:exon 20.0%
!! TTCACATTATTTAATCTTGA+GGG + chr6_1:52352630-52352649 Msa0888170:intron 20.0%
!! TCAAATTATGACGTAAAAAA+GGG - chr6_1:52352672-52352691 None:intergenic 20.0%
!! TTCAAATTATGACGTAAAAA+AGG - chr6_1:52352673-52352692 None:intergenic 20.0%
!! TGTATTTCTTATACTTTCAA+TGG - chr6_1:52353310-52353329 None:intergenic 20.0%
!!! TTGAAGATGAAAAGTGTTAT+AGG - chr6_1:52352228-52352247 None:intergenic 25.0%
! AAAATTTAAAAGAAGAGCCA+GGG - chr6_1:52352268-52352287 None:intergenic 25.0%
! TAAAATTTAAAAGAAGAGCC+AGG - chr6_1:52352269-52352288 None:intergenic 25.0%
!! TTAACAGAAACTAGTATTGA+AGG + chr6_1:52352426-52352445 Msa0888170:intron 25.0%
!!! ACATGTCTAGTTTCAATTTT+TGG + chr6_1:52352518-52352537 Msa0888170:intron 25.0%
! CTCAAGATTAAATAATGTGA+AGG - chr6_1:52352631-52352650 None:intergenic 25.0%
! CTTCACATTATTTAATCTTG+AGG + chr6_1:52352629-52352648 Msa0888170:intron 25.0%
!! TGTATTGGTATATTATTTGC+AGG + chr6_1:52352840-52352859 Msa0888170:intron 25.0%
!! GTATTGGTATATTATTTGCA+GGG + chr6_1:52352841-52352860 Msa0888170:intron 25.0%
! TATTGGTATATTATTTGCAG+GGG + chr6_1:52352842-52352861 Msa0888170:intron 25.0%
! AGACTTCAAGTAATTGAATT+TGG - chr6_1:52353040-52353059 None:intergenic 25.0%
! TGTTGCATATGATAATAATG+AGG - chr6_1:52353065-52353084 None:intergenic 25.0%
! GAGGAATATTGAATATATTG+TGG - chr6_1:52353089-52353108 None:intergenic 25.0%
!!! TTGAAGATGAAAAGTGTTAT+AGG - chr6_1:52352228-52352247 None:intergenic 25.0%
! AAAATTTAAAAGAAGAGCCA+GGG - chr6_1:52352268-52352287 None:intergenic 25.0%
! TAAAATTTAAAAGAAGAGCC+AGG - chr6_1:52352269-52352288 None:intergenic 25.0%
!! TTAACAGAAACTAGTATTGA+AGG + chr6_1:52352426-52352445 Msa0888170:intron 25.0%
!!! ACATGTCTAGTTTCAATTTT+TGG + chr6_1:52352518-52352537 Msa0888170:intron 25.0%
! CTCAAGATTAAATAATGTGA+AGG - chr6_1:52352631-52352650 None:intergenic 25.0%
! CTTCACATTATTTAATCTTG+AGG + chr6_1:52352629-52352648 Msa0888170:intron 25.0%
!! TGTATTGGTATATTATTTGC+AGG + chr6_1:52352840-52352859 Msa0888170:intron 25.0%
!! GTATTGGTATATTATTTGCA+GGG + chr6_1:52352841-52352860 Msa0888170:intron 25.0%
! TATTGGTATATTATTTGCAG+GGG + chr6_1:52352842-52352861 Msa0888170:intron 25.0%
! AGACTTCAAGTAATTGAATT+TGG - chr6_1:52353040-52353059 None:intergenic 25.0%
! TGTTGCATATGATAATAATG+AGG - chr6_1:52353065-52353084 None:intergenic 25.0%
! GAGGAATATTGAATATATTG+TGG - chr6_1:52353089-52353108 None:intergenic 25.0%
GACAAGAAATTAACCTTAAC+TGG + chr6_1:52352891-52352910 Msa0888170:CDS 30.0%
TACATGTAGTAAGTAAGCTA+AGG + chr6_1:52352928-52352947 Msa0888170:CDS 30.0%
TAGTAAGTAAGCTAAGGAAA+TGG + chr6_1:52352934-52352953 Msa0888170:CDS 30.0%
ATGCTGAAATAGTTTCTGTT+GGG + chr6_1:52352961-52352980 Msa0888170:CDS 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG - chr6_1:52352987-52353006 None:intergenic 30.0%
ATATTCCTCTGTTTCAAGAA+TGG + chr6_1:52353100-52353119 Msa0888170:CDS 30.0%
GACAAGAAATTAACCTTAAC+TGG + chr6_1:52352891-52352910 Msa0888170:CDS 30.0%
TACATGTAGTAAGTAAGCTA+AGG + chr6_1:52352928-52352947 Msa0888170:CDS 30.0%
TAGTAAGTAAGCTAAGGAAA+TGG + chr6_1:52352934-52352953 Msa0888170:CDS 30.0%
ATGCTGAAATAGTTTCTGTT+GGG + chr6_1:52352961-52352980 Msa0888170:CDS 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG - chr6_1:52352987-52353006 None:intergenic 30.0%
ATATTCCTCTGTTTCAAGAA+TGG + chr6_1:52353100-52353119 Msa0888170:CDS 30.0%
! AACCAACAGCTCTGATTTAT+TGG + chr6_1:52352288-52352307 Msa0888170:five_prime_UTR 35.0%
AGTAAAAGAGGGAATTCACA+AGG - chr6_1:52352358-52352377 None:intergenic 35.0%
! ACAGAAACTAGTATTGAAGG+TGG + chr6_1:52352429-52352448 Msa0888170:intron 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG - chr6_1:52352473-52352492 None:intergenic 35.0%
AGCTTACTTACTACATGTAC+TGG - chr6_1:52352927-52352946 None:intergenic 35.0%
CATGCTGAAATAGTTTCTGT+TGG + chr6_1:52352960-52352979 Msa0888170:CDS 35.0%
GAAGAAAGAAGAATCTAAGC+CGG + chr6_1:52353001-52353020 Msa0888170:CDS 35.0%
TTGTTCCATTCTTGAAACAG+AGG - chr6_1:52353108-52353127 None:intergenic 35.0%
GTGTTCAATTGTGCAAACAA+AGG + chr6_1:52353262-52353281 Msa0888170:three_prime_UTR 35.0%
! AACCAACAGCTCTGATTTAT+TGG + chr6_1:52352288-52352307 Msa0888170:five_prime_UTR 35.0%
AGTAAAAGAGGGAATTCACA+AGG - chr6_1:52352358-52352377 None:intergenic 35.0%
! ACAGAAACTAGTATTGAAGG+TGG + chr6_1:52352429-52352448 Msa0888170:intron 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG - chr6_1:52352473-52352492 None:intergenic 35.0%
AGCTTACTTACTACATGTAC+TGG - chr6_1:52352927-52352946 None:intergenic 35.0%
CATGCTGAAATAGTTTCTGT+TGG + chr6_1:52352960-52352979 Msa0888170:CDS 35.0%
GAAGAAAGAAGAATCTAAGC+CGG + chr6_1:52353001-52353020 Msa0888170:CDS 35.0%
TTGTTCCATTCTTGAAACAG+AGG - chr6_1:52353108-52353127 None:intergenic 35.0%
GTGTTCAATTGTGCAAACAA+AGG + chr6_1:52353262-52353281 Msa0888170:three_prime_UTR 35.0%
ATTCTATAGCTCCAATACCC+TGG + chr6_1:52352248-52352267 Msa0888170:five_prime_UTR 40.0%
TAAAAGAAGAGCCAGGGTAT+TGG - chr6_1:52352262-52352281 None:intergenic 40.0%
GACCAATAAATCAGAGCTGT+TGG - chr6_1:52352293-52352312 None:intergenic 40.0%
AAAGCTATGAAGACAGTCTC+TGG + chr6_1:52352478-52352497 Msa0888170:CDS 40.0%
ACATGTCGACATTACCTGAA+AGG - chr6_1:52352504-52352523 None:intergenic 40.0%
ATTTGGTCCTTCTTCACATC+CGG - chr6_1:52353023-52353042 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG + chr6_1:52353116-52353135 Msa0888170:CDS 40.0%
ATTCTATAGCTCCAATACCC+TGG + chr6_1:52352248-52352267 Msa0888170:five_prime_UTR 40.0%
TAAAAGAAGAGCCAGGGTAT+TGG - chr6_1:52352262-52352281 None:intergenic 40.0%
GACCAATAAATCAGAGCTGT+TGG - chr6_1:52352293-52352312 None:intergenic 40.0%
AAAGCTATGAAGACAGTCTC+TGG + chr6_1:52352478-52352497 Msa0888170:CDS 40.0%
ACATGTCGACATTACCTGAA+AGG - chr6_1:52352504-52352523 None:intergenic 40.0%
ATTTGGTCCTTCTTCACATC+CGG - chr6_1:52353023-52353042 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG + chr6_1:52353116-52353135 Msa0888170:CDS 40.0%
TGGATCTGTGTCTCCAGTTA+AGG - chr6_1:52352907-52352926 None:intergenic 45.0%
ATCTAAGCCGGATGTGAAGA+AGG + chr6_1:52353013-52353032 Msa0888170:CDS 45.0%
CAGCATATGACACATCCAAC+AGG - chr6_1:52353134-52353153 None:intergenic 45.0%
TGGATCTGTGTCTCCAGTTA+AGG - chr6_1:52352907-52352926 None:intergenic 45.0%
ATCTAAGCCGGATGTGAAGA+AGG + chr6_1:52353013-52353032 Msa0888170:CDS 45.0%
CAGCATATGACACATCCAAC+AGG - chr6_1:52353134-52353153 None:intergenic 45.0%
!!! TTTATTTTTAAATTGAAAAA+TGG - chr6_1:52352731-52352750 None:intergenic 5.0%
!!! TTTATTTTTAAATTGAAAAA+TGG - chr6_1:52352731-52352750 None:intergenic 5.0%
! AAGACAGTCTCTGGCCTTTC+AGG + chr6_1:52352487-52352506 Msa0888170:CDS 50.0%
! AAGACAGTCTCTGGCCTTTC+AGG + chr6_1:52352487-52352506 Msa0888170:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 52352202 52353338 52352202 ID=Msa0888170;Name=Msa0888170
chr6_1 mRNA 52352202 52353338 52352202 ID=Msa0888170-mRNA-1;Parent=Msa0888170;Name=Msa0888170-mRNA-1;_AED=0.18;_eAED=0.18;_QI=134|1|1|1|1|1|3|184|124
chr6_1 exon 52352202 52352341 52352202 ID=Msa0888170-mRNA-1:exon:10516;Parent=Msa0888170-mRNA-1
chr6_1 exon 52352433 52352508 52352433 ID=Msa0888170-mRNA-1:exon:10517;Parent=Msa0888170-mRNA-1
chr6_1 exon 52352862 52353338 52352862 ID=Msa0888170-mRNA-1:exon:10518;Parent=Msa0888170-mRNA-1
chr6_1 five_prime_UTR 52352202 52352335 52352202 ID=Msa0888170-mRNA-1:five_prime_utr;Parent=Msa0888170-mRNA-1
chr6_1 CDS 52352336 52352341 52352336 ID=Msa0888170-mRNA-1:cds;Parent=Msa0888170-mRNA-1
chr6_1 CDS 52352433 52352508 52352433 ID=Msa0888170-mRNA-1:cds;Parent=Msa0888170-mRNA-1
chr6_1 CDS 52352862 52353154 52352862 ID=Msa0888170-mRNA-1:cds;Parent=Msa0888170-mRNA-1
chr6_1 three_prime_UTR 52353155 52353338 52353155 ID=Msa0888170-mRNA-1:three_prime_utr;Parent=Msa0888170-mRNA-1
Gene Sequence

>Msa0888170

ATGATGAAACTAGTATTGAAGGTGGATTTACATGATGATAGAACCAAGCAAAAAGCTATGAAGACAGTCTCTGGCCTTTCAGGGGTAGAGTCAGTTTCTATAGACATGAAAGACAAGAAATTAACCTTAACTGGAGACACAGATCCAGTACATGTAGTAAGTAAGCTAAGGAAATGGTGTCATGCTGAAATAGTTTCTGTTGGGCCTGCAAAAGATGAAAAGAAGAAAGAAGAATCTAAGCCGGATGTGAAGAAGGACCAAATTCAATTACTTGAAGTCTATCCTCATTATTATCATATGCAACAACCACAATATATTCAATATTCCTCTGTTTCAAGAATGGAACAAGATCCTGTTGGATGTGTCATATGCTGA

Protein sequence

>Msa0888170

MMKLVLKVDLHDDRTKQKAMKTVSGLSGVESVSIDMKDKKLTLTGDTDPVHVVSKLRKWCHAEIVSVGPAKDEKKKEESKPDVKKDQIQLLEVYPHYYHMQQPQYIQYSSVSRMEQDPVGCVIC*