AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0914290


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0914290 MtrunA17_Chr6g0469571 95.968 124 5 0 1 124 1 124 3.62e-75 218
Msa0914290 MtrunA17_Chr6g0469551 94.355 124 7 0 1 124 1 124 1.31e-74 217
Msa0914290 MtrunA17_Chr6g0469591 94.355 124 7 0 1 124 1 124 1.70e-73 214
Msa0914290 MtrunA17_Chr6g0469681 61.240 129 43 3 1 124 1 127 6.57e-39 127
Msa0914290 MtrunA17_Chr1g0202541 57.037 135 46 2 1 123 1 135 1.75e-37 124
Msa0914290 MtrunA17_Chr7g0233231 56.481 108 45 1 1 108 1 106 4.41e-37 122
Msa0914290 MtrunA17_Chr6g0469651 56.716 134 47 2 1 124 1 133 1.49e-36 121
Msa0914290 MtrunA17_Chr7g0233211 56.075 107 45 1 1 107 1 105 5.69e-36 119
Msa0914290 MtrunA17_Chr1g0202561 52.174 138 51 2 1 123 1 138 1.06e-35 119
Msa0914290 MtrunA17_Chr7g0233251 56.140 114 45 2 1 111 1 112 2.14e-35 117
Msa0914290 MtrunA17_Chr7g0233191 56.075 107 45 1 1 107 1 105 4.54e-35 117
Msa0914290 MtrunA17_Chr7g0233201 56.075 107 45 1 1 107 1 105 9.55e-35 116
Msa0914290 MtrunA17_Chr6g0469541 57.944 107 43 2 1 107 1 105 1.55e-34 115
Msa0914290 MtrunA17_Chr6g0469671 78.571 70 15 0 1 70 1 70 1.57e-34 115
Msa0914290 MtrunA17_Chr1g0202581 55.556 135 44 3 1 123 1 131 5.13e-34 115
Msa0914290 MtrunA17_Chr7g0233181 54.717 106 46 1 1 106 1 104 7.06e-34 113
Msa0914290 MtrunA17_Chr8g0387471 49.618 131 55 3 1 124 1 127 1.39e-33 113
Msa0914290 MtrunA17_Chr1g0204291 52.593 135 48 4 1 123 1 131 2.26e-32 110
Msa0914290 MtrunA17_Chr1g0202501 49.254 134 54 3 1 124 1 130 4.01e-32 110
Msa0914290 MtrunA17_Chr7g0248671 61.765 68 26 0 1 68 1 68 3.15e-24 91.3
Msa0914290 MtrunA17_Chr8g0348361 67.647 68 22 0 1 68 1 68 1.44e-15 68.2
Msa0914290 MtrunA17_Chr3g0120541 41.975 81 43 4 3 79 5 85 8.07e-13 60.5
Msa0914290 MtrunA17_Chr5g0404641 30.719 153 74 4 3 123 4 156 2.34e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0914290 AT1G01490.1 67.606 71 22 1 1 70 1 71 1.32e-25 95.1
Msa0914290 AT1G01490.2 67.606 71 22 1 1 70 1 71 1.32e-25 95.1
Msa0914290 AT1G01490.4 67.143 70 22 1 2 70 29 98 4.92e-25 94.4
Msa0914290 AT5G52740.1 55.714 70 29 1 1 70 1 68 4.14e-18 74.3
Msa0914290 AT5G52760.1 43.269 104 49 4 5 105 7 103 1.40e-16 70.9
Msa0914290 AT5G52750.1 45.455 66 36 0 2 67 4 69 1.93e-13 62.8
Msa0914290 AT5G23760.1 52.174 69 32 1 3 70 4 72 2.95e-11 56.2
Msa0914290 AT1G63960.2 35.714 70 44 1 1 69 11 80 6.14e-11 56.6
Msa0914290 AT1G63950.2 30.400 125 77 3 1 124 1 116 6.36e-11 55.8

Find 27 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACAACAATTCTGATTTAT+TGG 0.145112 6_2:+27772592 Msa0914290:five_prime_UTR
AAGACAGTCTCTGGCCTTTC+AGG 0.146342 6_2:+27772794 Msa0914290:CDS
GACAAGAAATTAACCTTAAT+TGG 0.151973 6_2:+27773435 Msa0914290:CDS
TTATATTTCATATGATTTAT+TGG 0.157338 6_2:+27773724 Msa0914290:three_prime_UTR
TGGATCTGTGTCTCCAATTA+AGG 0.276564 6_2:-27773448 None:intergenic
TTATTGGATTTGTAACATAT+TGG 0.298794 6_2:+27773740 Msa0914290:three_prime_UTR
ATTTGATTCTTCTTCACATC+AGG 0.383077 6_2:-27773564 None:intergenic
ATATTCCTCTGTTTCAAGAA+TGG 0.394512 6_2:+27773644 Msa0914290:CDS
TAAAAGAAGAGCTAGGGTAT+TGG 0.399065 6_2:-27772563 None:intergenic
TAAAATTTAAAAGAAGAGCT+AGG 0.408169 6_2:-27772570 None:intergenic
AGTTTACTTACTACATGTAC+TGG 0.438622 6_2:-27773468 None:intergenic
AAAAGCTTTATTGAAAATGA+TGG 0.441720 6_2:+27772620 Msa0914290:exon
GTTACAGAAACTAGTATTGA+AGG 0.451235 6_2:+27772733 Msa0914290:intron
TAGTAAGTAAACTAAGGAAA+TGG 0.475788 6_2:+27773478 Msa0914290:CDS
ATGCTGAAATAGTTTCTGTT+GGG 0.476513 6_2:+27773505 Msa0914290:CDS
AAAGCTATGAAGACAGTCTC+TGG 0.487579 6_2:+27772785 Msa0914290:CDS
AAAATTTAAAAGAAGAGCTA+GGG 0.497403 6_2:-27772569 None:intergenic
CATGCTGAAATAGTTTCTGT+TGG 0.500770 6_2:+27773504 Msa0914290:CDS
TACATGTAGTAAGTAAACTA+AGG 0.515410 6_2:+27773472 Msa0914290:CDS
CAGCATATGACACATCCAAC+AGG 0.518380 6_2:-27773675 None:intergenic
GAGGAATATTGAATATATTG+TGG 0.535752 6_2:-27773630 None:intergenic
TTGTTCCATTCTTGAAACAG+AGG 0.577364 6_2:-27773649 None:intergenic
CATGTAGTAATAATGAGGAT+AGG 0.591628 6_2:-27773601 None:intergenic
ACATATTGACATTACCTGAA+AGG 0.607504 6_2:-27772808 None:intergenic
AGAATGGAACAAGATCCTGT+TGG 0.628668 6_2:+27773660 Msa0914290:CDS
TGTTGCATGTAGTAATAATG+AGG 0.650568 6_2:-27773606 None:intergenic
ACAGAAACTAGTATTGAAGG+TGG 0.658613 6_2:+27772736 Msa0914290:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTTAAAAAATTGAAAAA+TGG - chr6_2:27773072-27773091 None:intergenic 10.0%
!! TAATAATATATTTATCTACA+CGG + chr6_2:27773264-27773283 Msa0914290:intron 10.0%
!! TTATATTTCATATGATTTAT+TGG + chr6_2:27773724-27773743 Msa0914290:three_prime_UTR 10.0%
!! TTTGTTAAAAAATTGAAAAA+TGG - chr6_2:27773072-27773091 None:intergenic 10.0%
!! TAATAATATATTTATCTACA+CGG + chr6_2:27773264-27773283 Msa0914290:intron 10.0%
!! TTATATTTCATATGATTTAT+TGG + chr6_2:27773724-27773743 Msa0914290:three_prime_UTR 10.0%
!! AATAATGCATGATTAAAATA+TGG - chr6_2:27772938-27772957 None:intergenic 15.0%
!!! AATTTTAGCAAAAAACTTAA+AGG - chr6_2:27773365-27773384 None:intergenic 15.0%
!! AACATATTGGAAAAAATAAA+AGG + chr6_2:27773753-27773772 Msa0914290:three_prime_UTR 15.0%
!! AATAATGCATGATTAAAATA+TGG - chr6_2:27772938-27772957 None:intergenic 15.0%
!!! AATTTTAGCAAAAAACTTAA+AGG - chr6_2:27773365-27773384 None:intergenic 15.0%
!! AACATATTGGAAAAAATAAA+AGG + chr6_2:27773753-27773772 Msa0914290:three_prime_UTR 15.0%
!! AAAATTTAAAAGAAGAGCTA+GGG - chr6_2:27772572-27772591 None:intergenic 20.0%
!! TAAAATTTAAAAGAAGAGCT+AGG - chr6_2:27772573-27772592 None:intergenic 20.0%
!! AAACAACAATTCTGATTTAT+TGG + chr6_2:27772592-27772611 Msa0914290:five_prime_UTR 20.0%
!!! AAAAGCTTTATTGAAAATGA+TGG + chr6_2:27772620-27772639 Msa0914290:exon 20.0%
!!! TAAAATATGGTTACATTTTC+TGG - chr6_2:27772925-27772944 None:intergenic 20.0%
!!! TTTGTGTTTTTCAGTTTATA+AGG + chr6_2:27772975-27772994 Msa0914290:intron 20.0%
!! TTAAACTCATGATATAACAT+TGG + chr6_2:27773335-27773354 Msa0914290:intron 20.0%
!!! GTTTTTTGCTAAAATTGTAT+TGG + chr6_2:27773369-27773388 Msa0914290:intron 20.0%
!! TTATTGGATTTGTAACATAT+TGG + chr6_2:27773740-27773759 Msa0914290:three_prime_UTR 20.0%
!! AAAATTTAAAAGAAGAGCTA+GGG - chr6_2:27772572-27772591 None:intergenic 20.0%
!! TAAAATTTAAAAGAAGAGCT+AGG - chr6_2:27772573-27772592 None:intergenic 20.0%
!! AAACAACAATTCTGATTTAT+TGG + chr6_2:27772592-27772611 Msa0914290:five_prime_UTR 20.0%
!!! AAAAGCTTTATTGAAAATGA+TGG + chr6_2:27772620-27772639 Msa0914290:exon 20.0%
!!! TAAAATATGGTTACATTTTC+TGG - chr6_2:27772925-27772944 None:intergenic 20.0%
!!! TTTGTGTTTTTCAGTTTATA+AGG + chr6_2:27772975-27772994 Msa0914290:intron 20.0%
!! TTAAACTCATGATATAACAT+TGG + chr6_2:27773335-27773354 Msa0914290:intron 20.0%
!!! GTTTTTTGCTAAAATTGTAT+TGG + chr6_2:27773369-27773388 Msa0914290:intron 20.0%
!! TTATTGGATTTGTAACATAT+TGG + chr6_2:27773740-27773759 Msa0914290:three_prime_UTR 20.0%
! TAAATGATGTTCAAATTGTG+AGG - chr6_2:27773023-27773042 None:intergenic 25.0%
!! TGTATTGGTATAATATTTGC+AGG + chr6_2:27773384-27773403 Msa0914290:intron 25.0%
!! GTATTGGTATAATATTTGCA+GGG + chr6_2:27773385-27773404 Msa0914290:intron 25.0%
! TATTGGTATAATATTTGCAG+GGG + chr6_2:27773386-27773405 Msa0914290:intron 25.0%
! GACAAGAAATTAACCTTAAT+TGG + chr6_2:27773435-27773454 Msa0914290:CDS 25.0%
! TACATGTAGTAAGTAAACTA+AGG + chr6_2:27773472-27773491 Msa0914290:CDS 25.0%
! TAGTAAGTAAACTAAGGAAA+TGG + chr6_2:27773478-27773497 Msa0914290:CDS 25.0%
! GAGGAATATTGAATATATTG+TGG - chr6_2:27773633-27773652 None:intergenic 25.0%
! TAAATGATGTTCAAATTGTG+AGG - chr6_2:27773023-27773042 None:intergenic 25.0%
!! TGTATTGGTATAATATTTGC+AGG + chr6_2:27773384-27773403 Msa0914290:intron 25.0%
!! GTATTGGTATAATATTTGCA+GGG + chr6_2:27773385-27773404 Msa0914290:intron 25.0%
! TATTGGTATAATATTTGCAG+GGG + chr6_2:27773386-27773405 Msa0914290:intron 25.0%
! GACAAGAAATTAACCTTAAT+TGG + chr6_2:27773435-27773454 Msa0914290:CDS 25.0%
! TACATGTAGTAAGTAAACTA+AGG + chr6_2:27773472-27773491 Msa0914290:CDS 25.0%
! TAGTAAGTAAACTAAGGAAA+TGG + chr6_2:27773478-27773497 Msa0914290:CDS 25.0%
! GAGGAATATTGAATATATTG+TGG - chr6_2:27773633-27773652 None:intergenic 25.0%
! GTTACAGAAACTAGTATTGA+AGG + chr6_2:27772733-27772752 Msa0914290:intron 30.0%
ACATATTGACATTACCTGAA+AGG - chr6_2:27772811-27772830 None:intergenic 30.0%
TCAGGTAATGTCAATATGTA+CGG + chr6_2:27772812-27772831 Msa0914290:intron 30.0%
!! GTGTTTTTCAGTTTATAAGG+TGG + chr6_2:27772978-27772997 Msa0914290:intron 30.0%
AGTTTACTTACTACATGTAC+TGG - chr6_2:27773471-27773490 None:intergenic 30.0%
ATGCTGAAATAGTTTCTGTT+GGG + chr6_2:27773505-27773524 Msa0914290:CDS 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG - chr6_2:27773531-27773550 None:intergenic 30.0%
ATTTGATTCTTCTTCACATC+AGG - chr6_2:27773567-27773586 None:intergenic 30.0%
CATGTAGTAATAATGAGGAT+AGG - chr6_2:27773604-27773623 None:intergenic 30.0%
TGTTGCATGTAGTAATAATG+AGG - chr6_2:27773609-27773628 None:intergenic 30.0%
ATATTCCTCTGTTTCAAGAA+TGG + chr6_2:27773644-27773663 Msa0914290:CDS 30.0%
! GTTACAGAAACTAGTATTGA+AGG + chr6_2:27772733-27772752 Msa0914290:intron 30.0%
ACATATTGACATTACCTGAA+AGG - chr6_2:27772811-27772830 None:intergenic 30.0%
TCAGGTAATGTCAATATGTA+CGG + chr6_2:27772812-27772831 Msa0914290:intron 30.0%
!! GTGTTTTTCAGTTTATAAGG+TGG + chr6_2:27772978-27772997 Msa0914290:intron 30.0%
AGTTTACTTACTACATGTAC+TGG - chr6_2:27773471-27773490 None:intergenic 30.0%
ATGCTGAAATAGTTTCTGTT+GGG + chr6_2:27773505-27773524 Msa0914290:CDS 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG - chr6_2:27773531-27773550 None:intergenic 30.0%
ATTTGATTCTTCTTCACATC+AGG - chr6_2:27773567-27773586 None:intergenic 30.0%
CATGTAGTAATAATGAGGAT+AGG - chr6_2:27773604-27773623 None:intergenic 30.0%
TGTTGCATGTAGTAATAATG+AGG - chr6_2:27773609-27773628 None:intergenic 30.0%
ATATTCCTCTGTTTCAAGAA+TGG + chr6_2:27773644-27773663 Msa0914290:CDS 30.0%
TAAAAGAAGAGCTAGGGTAT+TGG - chr6_2:27772566-27772585 None:intergenic 35.0%
! ACAGAAACTAGTATTGAAGG+TGG + chr6_2:27772736-27772755 Msa0914290:intron 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG - chr6_2:27772780-27772799 None:intergenic 35.0%
CATGCTGAAATAGTTTCTGT+TGG + chr6_2:27773504-27773523 Msa0914290:CDS 35.0%
TTGTTCCATTCTTGAAACAG+AGG - chr6_2:27773652-27773671 None:intergenic 35.0%
TAAAAGAAGAGCTAGGGTAT+TGG - chr6_2:27772566-27772585 None:intergenic 35.0%
! ACAGAAACTAGTATTGAAGG+TGG + chr6_2:27772736-27772755 Msa0914290:intron 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG - chr6_2:27772780-27772799 None:intergenic 35.0%
CATGCTGAAATAGTTTCTGT+TGG + chr6_2:27773504-27773523 Msa0914290:CDS 35.0%
TTGTTCCATTCTTGAAACAG+AGG - chr6_2:27773652-27773671 None:intergenic 35.0%
AAAGCTATGAAGACAGTCTC+TGG + chr6_2:27772785-27772804 Msa0914290:CDS 40.0%
ACCGAGGTAGTAAAATGTTG+CGG + chr6_2:27773207-27773226 Msa0914290:intron 40.0%
! TCCGCAACATTTTACTACCT+CGG - chr6_2:27773211-27773230 None:intergenic 40.0%
TGGATCTGTGTCTCCAATTA+AGG - chr6_2:27773451-27773470 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG + chr6_2:27773660-27773679 Msa0914290:CDS 40.0%
AAAGCTATGAAGACAGTCTC+TGG + chr6_2:27772785-27772804 Msa0914290:CDS 40.0%
ACCGAGGTAGTAAAATGTTG+CGG + chr6_2:27773207-27773226 Msa0914290:intron 40.0%
! TCCGCAACATTTTACTACCT+CGG - chr6_2:27773211-27773230 None:intergenic 40.0%
TGGATCTGTGTCTCCAATTA+AGG - chr6_2:27773451-27773470 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG + chr6_2:27773660-27773679 Msa0914290:CDS 40.0%
CAGCATATGACACATCCAAC+AGG - chr6_2:27773678-27773697 None:intergenic 45.0%
CAGCATATGACACATCCAAC+AGG - chr6_2:27773678-27773697 None:intergenic 45.0%
! AAGACAGTCTCTGGCCTTTC+AGG + chr6_2:27772794-27772813 Msa0914290:CDS 50.0%
AATCCGCGTAAGTGTAACCG+AGG + chr6_2:27773191-27773210 Msa0914290:intron 50.0%
CTACCTCGGTTACACTTACG+CGG - chr6_2:27773197-27773216 None:intergenic 50.0%
! AAGACAGTCTCTGGCCTTTC+AGG + chr6_2:27772794-27772813 Msa0914290:CDS 50.0%
AATCCGCGTAAGTGTAACCG+AGG + chr6_2:27773191-27773210 Msa0914290:intron 50.0%
CTACCTCGGTTACACTTACG+CGG - chr6_2:27773197-27773216 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 27772507 27773874 27772507 ID=Msa0914290;Name=Msa0914290
chr6_2 mRNA 27772507 27773874 27772507 ID=Msa0914290-mRNA-1;Parent=Msa0914290;Name=Msa0914290-mRNA-1;_AED=0.07;_eAED=0.07;_QI=129|1|1|1|1|1|3|176|124
chr6_2 exon 27772507 27772641 27772507 ID=Msa0914290-mRNA-1:exon:5135;Parent=Msa0914290-mRNA-1
chr6_2 exon 27772740 27772815 27772740 ID=Msa0914290-mRNA-1:exon:5136;Parent=Msa0914290-mRNA-1
chr6_2 exon 27773406 27773874 27773406 ID=Msa0914290-mRNA-1:exon:5137;Parent=Msa0914290-mRNA-1
chr6_2 five_prime_UTR 27772507 27772635 27772507 ID=Msa0914290-mRNA-1:five_prime_utr;Parent=Msa0914290-mRNA-1
chr6_2 CDS 27772636 27772641 27772636 ID=Msa0914290-mRNA-1:cds;Parent=Msa0914290-mRNA-1
chr6_2 CDS 27772740 27772815 27772740 ID=Msa0914290-mRNA-1:cds;Parent=Msa0914290-mRNA-1
chr6_2 CDS 27773406 27773698 27773406 ID=Msa0914290-mRNA-1:cds;Parent=Msa0914290-mRNA-1
chr6_2 three_prime_UTR 27773699 27773874 27773699 ID=Msa0914290-mRNA-1:three_prime_utr;Parent=Msa0914290-mRNA-1
Gene Sequence

>Msa0914290

ATGATGAAACTAGTATTGAAGGTGGATTTACATGATGACAGAACCAAGCAAAAAGCTATGAAGACAGTCTCTGGCCTTTCAGGGGTAGAGTCAGTTTCTGTAGACATGAAAGACAAGAAATTAACCTTAATTGGAGACACAGATCCAGTACATGTAGTAAGTAAACTAAGGAAATGGTGTCATGCTGAAATAGTTTCTGTTGGGCCTGCAAAAGATGAAAAGAAGAAAGAAGAATCTAAGCCTGATGTGAAGAAGAATCAAATTCAATTACTTGAAGCCTATCCTCATTATTACTACATGCAACAACCACAATATATTCAATATTCCTCTGTTTCAAGAATGGAACAAGATCCTGTTGGATGTGTCATATGCTGA

Protein sequence

>Msa0914290

MMKLVLKVDLHDDRTKQKAMKTVSGLSGVESVSVDMKDKKLTLIGDTDPVHVVSKLRKWCHAEIVSVGPAKDEKKKEESKPDVKKNQIQLLEAYPHYYYMQQPQYIQYSSVSRMEQDPVGCVIC*