AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0952360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0952360 MtrunA17_Chr6g0469571 95.161 124 6 0 1 124 1 124 3.54e-74 216
Msa0952360 MtrunA17_Chr6g0469551 93.548 124 8 0 1 124 1 124 1.25e-73 214
Msa0952360 MtrunA17_Chr6g0469591 93.548 124 8 0 1 124 1 124 1.28e-72 212
Msa0952360 MtrunA17_Chr6g0469681 61.240 129 43 3 1 124 1 127 9.85e-39 126
Msa0952360 MtrunA17_Chr1g0202541 57.037 135 46 2 1 123 1 135 5.05e-37 122
Msa0952360 MtrunA17_Chr7g0233231 56.481 108 45 1 1 108 1 106 5.67e-37 121
Msa0952360 MtrunA17_Chr6g0469651 56.716 134 47 2 1 124 1 133 3.23e-36 120
Msa0952360 MtrunA17_Chr7g0233211 56.075 107 45 1 1 107 1 105 7.40e-36 119
Msa0952360 MtrunA17_Chr7g0233251 57.009 107 44 1 1 107 1 105 3.31e-35 117
Msa0952360 MtrunA17_Chr1g0202561 53.237 139 48 3 1 123 1 138 3.97e-35 118
Msa0952360 MtrunA17_Chr7g0233191 56.075 107 45 1 1 107 1 105 5.59e-35 116
Msa0952360 MtrunA17_Chr1g0202581 54.815 135 45 3 1 123 1 131 9.98e-35 116
Msa0952360 MtrunA17_Chr7g0233201 56.075 107 45 1 1 107 1 105 1.33e-34 115
Msa0952360 MtrunA17_Chr6g0469541 57.944 107 43 2 1 107 1 105 1.82e-34 115
Msa0952360 MtrunA17_Chr6g0469671 78.571 70 15 0 1 70 1 70 1.83e-34 115
Msa0952360 MtrunA17_Chr7g0233181 54.717 106 46 1 1 106 1 104 9.07e-34 113
Msa0952360 MtrunA17_Chr8g0387471 49.618 131 55 3 1 124 1 127 2.74e-33 112
Msa0952360 MtrunA17_Chr1g0202501 47.761 134 56 2 1 124 1 130 2.18e-31 108
Msa0952360 MtrunA17_Chr1g0204291 51.852 135 49 4 1 123 1 131 2.33e-31 108
Msa0952360 MtrunA17_Chr7g0248671 61.765 68 26 0 1 68 1 68 2.92e-24 91.3
Msa0952360 MtrunA17_Chr8g0348361 67.647 68 22 0 1 68 1 68 1.21e-15 68.6
Msa0952360 MtrunA17_Chr3g0120541 41.975 81 43 4 3 79 5 85 8.70e-13 60.5
Msa0952360 MtrunA17_Chr5g0404641 30.719 153 74 4 3 123 4 156 4.45e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0952360 AT1G01490.1 67.606 71 22 1 1 70 1 71 1.20e-25 95.5
Msa0952360 AT1G01490.2 67.606 71 22 1 1 70 1 71 1.20e-25 95.5
Msa0952360 AT1G01490.4 67.143 70 22 1 2 70 29 98 4.82e-25 94.4
Msa0952360 AT5G52740.1 55.714 70 29 1 1 70 1 68 6.52e-18 73.9
Msa0952360 AT5G52760.1 43.269 104 49 4 5 105 7 103 1.34e-16 70.9
Msa0952360 AT5G52750.1 45.455 66 36 0 2 67 4 69 2.74e-13 62.4
Msa0952360 AT5G23760.1 52.174 69 32 1 3 70 4 72 2.86e-11 56.6
Msa0952360 AT1G63960.2 35.714 70 44 1 1 69 11 80 6.41e-11 56.6
Msa0952360 AT1G63950.2 30.400 125 77 3 1 124 1 116 6.78e-11 55.8

Find 27 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACAACAATTCTGATTTAT+TGG 0.145112 6_3:+46605281 Msa0952360:five_prime_UTR
AAGACAGTCTCTGGCCTTTC+AGG 0.146342 6_3:+46605483 Msa0952360:CDS
GACAAGAAATTAACCTTAAT+TGG 0.151973 6_3:+46606126 Msa0952360:CDS
TTATATTTCATATGATTTAT+TGG 0.157338 6_3:+46606415 Msa0952360:three_prime_UTR
TGGATCTGTGTCTCCAATTA+AGG 0.276564 6_3:-46606139 None:intergenic
ATTTGATTCTTCTTCACATC+AGG 0.383077 6_3:-46606255 None:intergenic
TAAAAGAAGAGCTAGGGTAT+TGG 0.399065 6_3:-46605252 None:intergenic
ATATTCCTGTGTTTCAAGAA+TGG 0.407778 6_3:+46606335 Msa0952360:CDS
TAAAATTTAAAAGAAGAGCT+AGG 0.408169 6_3:-46605259 None:intergenic
AGTTTACTTACTACATGTAC+TGG 0.438622 6_3:-46606159 None:intergenic
AAAAGCTTTATTGAAAATGA+TGG 0.441720 6_3:+46605309 Msa0952360:exon
GTTACAGAAACTAGTATTGA+AGG 0.451235 6_3:+46605422 Msa0952360:intron
TAGTAAGTAAACTAAGGAAA+TGG 0.475788 6_3:+46606169 Msa0952360:CDS
ATGCTGAAATAGTTTCTGTT+GGG 0.476513 6_3:+46606196 Msa0952360:CDS
AAAGCTATGAAGACAGTCTC+TGG 0.487579 6_3:+46605474 Msa0952360:CDS
AAAATTTAAAAGAAGAGCTA+GGG 0.497403 6_3:-46605258 None:intergenic
CATGCTGAAATAGTTTCTGT+TGG 0.500770 6_3:+46606195 Msa0952360:CDS
TACATGTAGTAAGTAAACTA+AGG 0.515410 6_3:+46606163 Msa0952360:CDS
CAGCATATGACACATCCAAC+AGG 0.518380 6_3:-46606366 None:intergenic
TTGTTCCATTCTTGAAACAC+AGG 0.547137 6_3:-46606340 None:intergenic
CAGGAATATTGAATATATTG+TGG 0.566124 6_3:-46606321 None:intergenic
TTATTGGATTTGCAACATGT+TGG 0.572098 6_3:+46606431 Msa0952360:three_prime_UTR
CATGTAGTAATAATGAGGAT+AGG 0.591628 6_3:-46606292 None:intergenic
ACATATTGACATTACCTGAA+AGG 0.607504 6_3:-46605497 None:intergenic
AGAATGGAACAAGATCCTGT+TGG 0.628668 6_3:+46606351 Msa0952360:CDS
TGTTGCATGTAGTAATAATG+AGG 0.650568 6_3:-46606297 None:intergenic
ACAGAAACTAGTATTGAAGG+TGG 0.658613 6_3:+46605425 Msa0952360:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTTAAAAAATTGAAAAA+TGG - chr6_3:46605761-46605780 None:intergenic 10.0%
!! TAATAATATATTTATCTACA+CGG + chr6_3:46605955-46605974 Msa0952360:intron 10.0%
!! TTATATTTCATATGATTTAT+TGG + chr6_3:46606415-46606434 Msa0952360:three_prime_UTR 10.0%
!! TTTGTTAAAAAATTGAAAAA+TGG - chr6_3:46605761-46605780 None:intergenic 10.0%
!! TAATAATATATTTATCTACA+CGG + chr6_3:46605955-46605974 Msa0952360:intron 10.0%
!! TTATATTTCATATGATTTAT+TGG + chr6_3:46606415-46606434 Msa0952360:three_prime_UTR 10.0%
!! AATAATGCATGATTAAAATA+TGG - chr6_3:46605627-46605646 None:intergenic 15.0%
!!! AATTTTAGCAAAAAACTTAA+AGG - chr6_3:46606056-46606075 None:intergenic 15.0%
!! AATAATGCATGATTAAAATA+TGG - chr6_3:46605627-46605646 None:intergenic 15.0%
!!! AATTTTAGCAAAAAACTTAA+AGG - chr6_3:46606056-46606075 None:intergenic 15.0%
!! AAAATTTAAAAGAAGAGCTA+GGG - chr6_3:46605261-46605280 None:intergenic 20.0%
!! TAAAATTTAAAAGAAGAGCT+AGG - chr6_3:46605262-46605281 None:intergenic 20.0%
!! AAACAACAATTCTGATTTAT+TGG + chr6_3:46605281-46605300 Msa0952360:five_prime_UTR 20.0%
!!! AAAAGCTTTATTGAAAATGA+TGG + chr6_3:46605309-46605328 Msa0952360:exon 20.0%
!!! TAAAATATGGTTACATTTTC+TGG - chr6_3:46605614-46605633 None:intergenic 20.0%
!!! TTTGTGTTTTTCAGTTTATA+AGG + chr6_3:46605664-46605683 Msa0952360:intron 20.0%
!! TTAAACTCATGATATAACAT+TGG + chr6_3:46606026-46606045 Msa0952360:intron 20.0%
!!! GTTTTTTGCTAAAATTGTAT+TGG + chr6_3:46606060-46606079 Msa0952360:intron 20.0%
!! AACATGTTGGAAAAAATAAA+AGG + chr6_3:46606444-46606463 Msa0952360:three_prime_UTR 20.0%
!! AAAATTTAAAAGAAGAGCTA+GGG - chr6_3:46605261-46605280 None:intergenic 20.0%
!! TAAAATTTAAAAGAAGAGCT+AGG - chr6_3:46605262-46605281 None:intergenic 20.0%
!! AAACAACAATTCTGATTTAT+TGG + chr6_3:46605281-46605300 Msa0952360:five_prime_UTR 20.0%
!!! AAAAGCTTTATTGAAAATGA+TGG + chr6_3:46605309-46605328 Msa0952360:exon 20.0%
!!! TAAAATATGGTTACATTTTC+TGG - chr6_3:46605614-46605633 None:intergenic 20.0%
!!! TTTGTGTTTTTCAGTTTATA+AGG + chr6_3:46605664-46605683 Msa0952360:intron 20.0%
!! TTAAACTCATGATATAACAT+TGG + chr6_3:46606026-46606045 Msa0952360:intron 20.0%
!!! GTTTTTTGCTAAAATTGTAT+TGG + chr6_3:46606060-46606079 Msa0952360:intron 20.0%
!! AACATGTTGGAAAAAATAAA+AGG + chr6_3:46606444-46606463 Msa0952360:three_prime_UTR 20.0%
! TAAATGATGTTCAAATTGTG+AGG - chr6_3:46605712-46605731 None:intergenic 25.0%
!! TGTATTGGTATAATATTTGC+AGG + chr6_3:46606075-46606094 Msa0952360:intron 25.0%
!! GTATTGGTATAATATTTGCA+GGG + chr6_3:46606076-46606095 Msa0952360:intron 25.0%
! TATTGGTATAATATTTGCAG+GGG + chr6_3:46606077-46606096 Msa0952360:intron 25.0%
! GACAAGAAATTAACCTTAAT+TGG + chr6_3:46606126-46606145 Msa0952360:CDS 25.0%
! TACATGTAGTAAGTAAACTA+AGG + chr6_3:46606163-46606182 Msa0952360:CDS 25.0%
! TAGTAAGTAAACTAAGGAAA+TGG + chr6_3:46606169-46606188 Msa0952360:CDS 25.0%
! CAGGAATATTGAATATATTG+TGG - chr6_3:46606324-46606343 None:intergenic 25.0%
! TAAATGATGTTCAAATTGTG+AGG - chr6_3:46605712-46605731 None:intergenic 25.0%
!! TGTATTGGTATAATATTTGC+AGG + chr6_3:46606075-46606094 Msa0952360:intron 25.0%
!! GTATTGGTATAATATTTGCA+GGG + chr6_3:46606076-46606095 Msa0952360:intron 25.0%
! TATTGGTATAATATTTGCAG+GGG + chr6_3:46606077-46606096 Msa0952360:intron 25.0%
! GACAAGAAATTAACCTTAAT+TGG + chr6_3:46606126-46606145 Msa0952360:CDS 25.0%
! TACATGTAGTAAGTAAACTA+AGG + chr6_3:46606163-46606182 Msa0952360:CDS 25.0%
! TAGTAAGTAAACTAAGGAAA+TGG + chr6_3:46606169-46606188 Msa0952360:CDS 25.0%
! CAGGAATATTGAATATATTG+TGG - chr6_3:46606324-46606343 None:intergenic 25.0%
! GTTACAGAAACTAGTATTGA+AGG + chr6_3:46605422-46605441 Msa0952360:intron 30.0%
ACATATTGACATTACCTGAA+AGG - chr6_3:46605500-46605519 None:intergenic 30.0%
TCAGGTAATGTCAATATGTA+CGG + chr6_3:46605501-46605520 Msa0952360:intron 30.0%
!! GTGTTTTTCAGTTTATAAGG+TGG + chr6_3:46605667-46605686 Msa0952360:intron 30.0%
AGTTTACTTACTACATGTAC+TGG - chr6_3:46606162-46606181 None:intergenic 30.0%
ATGCTGAAATAGTTTCTGTT+GGG + chr6_3:46606196-46606215 Msa0952360:CDS 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG - chr6_3:46606222-46606241 None:intergenic 30.0%
ATTTGATTCTTCTTCACATC+AGG - chr6_3:46606258-46606277 None:intergenic 30.0%
CATGTAGTAATAATGAGGAT+AGG - chr6_3:46606295-46606314 None:intergenic 30.0%
TGTTGCATGTAGTAATAATG+AGG - chr6_3:46606300-46606319 None:intergenic 30.0%
ATATTCCTGTGTTTCAAGAA+TGG + chr6_3:46606335-46606354 Msa0952360:CDS 30.0%
TTATTGGATTTGCAACATGT+TGG + chr6_3:46606431-46606450 Msa0952360:three_prime_UTR 30.0%
! GTTACAGAAACTAGTATTGA+AGG + chr6_3:46605422-46605441 Msa0952360:intron 30.0%
ACATATTGACATTACCTGAA+AGG - chr6_3:46605500-46605519 None:intergenic 30.0%
TCAGGTAATGTCAATATGTA+CGG + chr6_3:46605501-46605520 Msa0952360:intron 30.0%
!! GTGTTTTTCAGTTTATAAGG+TGG + chr6_3:46605667-46605686 Msa0952360:intron 30.0%
AGTTTACTTACTACATGTAC+TGG - chr6_3:46606162-46606181 None:intergenic 30.0%
ATGCTGAAATAGTTTCTGTT+GGG + chr6_3:46606196-46606215 Msa0952360:CDS 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG - chr6_3:46606222-46606241 None:intergenic 30.0%
ATTTGATTCTTCTTCACATC+AGG - chr6_3:46606258-46606277 None:intergenic 30.0%
CATGTAGTAATAATGAGGAT+AGG - chr6_3:46606295-46606314 None:intergenic 30.0%
TGTTGCATGTAGTAATAATG+AGG - chr6_3:46606300-46606319 None:intergenic 30.0%
ATATTCCTGTGTTTCAAGAA+TGG + chr6_3:46606335-46606354 Msa0952360:CDS 30.0%
TTATTGGATTTGCAACATGT+TGG + chr6_3:46606431-46606450 Msa0952360:three_prime_UTR 30.0%
TAAAAGAAGAGCTAGGGTAT+TGG - chr6_3:46605255-46605274 None:intergenic 35.0%
! ACAGAAACTAGTATTGAAGG+TGG + chr6_3:46605425-46605444 Msa0952360:intron 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG - chr6_3:46605469-46605488 None:intergenic 35.0%
!!! CGCAACATTTTTTACTACCT+CGG - chr6_3:46605900-46605919 None:intergenic 35.0%
CGAGGTAGTAAAAAATGTTG+CGG + chr6_3:46605898-46605917 Msa0952360:intron 35.0%
CATGCTGAAATAGTTTCTGT+TGG + chr6_3:46606195-46606214 Msa0952360:CDS 35.0%
TTGTTCCATTCTTGAAACAC+AGG - chr6_3:46606343-46606362 None:intergenic 35.0%
TAAAAGAAGAGCTAGGGTAT+TGG - chr6_3:46605255-46605274 None:intergenic 35.0%
! ACAGAAACTAGTATTGAAGG+TGG + chr6_3:46605425-46605444 Msa0952360:intron 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG - chr6_3:46605469-46605488 None:intergenic 35.0%
!!! CGCAACATTTTTTACTACCT+CGG - chr6_3:46605900-46605919 None:intergenic 35.0%
CGAGGTAGTAAAAAATGTTG+CGG + chr6_3:46605898-46605917 Msa0952360:intron 35.0%
CATGCTGAAATAGTTTCTGT+TGG + chr6_3:46606195-46606214 Msa0952360:CDS 35.0%
TTGTTCCATTCTTGAAACAC+AGG - chr6_3:46606343-46606362 None:intergenic 35.0%
AAAGCTATGAAGACAGTCTC+TGG + chr6_3:46605474-46605493 Msa0952360:CDS 40.0%
TGGATCTGTGTCTCCAATTA+AGG - chr6_3:46606142-46606161 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG + chr6_3:46606351-46606370 Msa0952360:CDS 40.0%
AAAGCTATGAAGACAGTCTC+TGG + chr6_3:46605474-46605493 Msa0952360:CDS 40.0%
TGGATCTGTGTCTCCAATTA+AGG - chr6_3:46606142-46606161 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG + chr6_3:46606351-46606370 Msa0952360:CDS 40.0%
CAGCATATGACACATCCAAC+AGG - chr6_3:46606369-46606388 None:intergenic 45.0%
CAGCATATGACACATCCAAC+AGG - chr6_3:46606369-46606388 None:intergenic 45.0%
! AAGACAGTCTCTGGCCTTTC+AGG + chr6_3:46605483-46605502 Msa0952360:CDS 50.0%
AATCCGCGTAAGTGTAACCG+AGG + chr6_3:46605880-46605899 Msa0952360:intron 50.0%
CTACCTCGGTTACACTTACG+CGG - chr6_3:46605886-46605905 None:intergenic 50.0%
! AAGACAGTCTCTGGCCTTTC+AGG + chr6_3:46605483-46605502 Msa0952360:CDS 50.0%
AATCCGCGTAAGTGTAACCG+AGG + chr6_3:46605880-46605899 Msa0952360:intron 50.0%
CTACCTCGGTTACACTTACG+CGG - chr6_3:46605886-46605905 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 46605196 46606565 46605196 ID=Msa0952360;Name=Msa0952360
chr6_3 mRNA 46605196 46606565 46605196 ID=Msa0952360-mRNA-1;Parent=Msa0952360;Name=Msa0952360-mRNA-1;_AED=0.07;_eAED=0.07;_QI=129|1|1|1|1|1|3|176|124
chr6_3 exon 46605196 46605330 46605196 ID=Msa0952360-mRNA-1:exon:10295;Parent=Msa0952360-mRNA-1
chr6_3 exon 46605429 46605504 46605429 ID=Msa0952360-mRNA-1:exon:10296;Parent=Msa0952360-mRNA-1
chr6_3 exon 46606097 46606565 46606097 ID=Msa0952360-mRNA-1:exon:10297;Parent=Msa0952360-mRNA-1
chr6_3 five_prime_UTR 46605196 46605324 46605196 ID=Msa0952360-mRNA-1:five_prime_utr;Parent=Msa0952360-mRNA-1
chr6_3 CDS 46605325 46605330 46605325 ID=Msa0952360-mRNA-1:cds;Parent=Msa0952360-mRNA-1
chr6_3 CDS 46605429 46605504 46605429 ID=Msa0952360-mRNA-1:cds;Parent=Msa0952360-mRNA-1
chr6_3 CDS 46606097 46606389 46606097 ID=Msa0952360-mRNA-1:cds;Parent=Msa0952360-mRNA-1
chr6_3 three_prime_UTR 46606390 46606565 46606390 ID=Msa0952360-mRNA-1:three_prime_utr;Parent=Msa0952360-mRNA-1
Gene Sequence

>Msa0952360

ATGATGAAACTAGTATTGAAGGTGGATTTACATGATGACAGAACCAAGCAAAAAGCTATGAAGACAGTCTCTGGCCTTTCAGGGGTAGAGTCAGTTTCTGTAGACATGAAAGACAAGAAATTAACCTTAATTGGAGACACAGATCCAGTACATGTAGTAAGTAAACTAAGGAAATGGTGTCATGCTGAAATAGTTTCTGTTGGGCCTGCAAAAGATGAAAAGAAGAAAGAAGAATCTAAGCCTGATGTGAAGAAGAATCAAATTCAATTACTTGAAGCCTATCCTCATTATTACTACATGCAACAACCACAATATATTCAATATTCCTGTGTTTCAAGAATGGAACAAGATCCTGTTGGATGTGTCATATGCTGA

Protein sequence

>Msa0952360

MMKLVLKVDLHDDRTKQKAMKTVSGLSGVESVSVDMKDKKLTLIGDTDPVHVVSKLRKWCHAEIVSVGPAKDEKKKEESKPDVKKNQIQLLEAYPHYYYMQQPQYIQYSCVSRMEQDPVGCVIC*