AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0892380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0892380 MtrunA17_Chr6g0474681 79.581 191 8 1 1 160 2 192 2.43e-105 299
Msa0892380 MtrunA17_Chr1g0204351 38.150 173 78 6 1 144 71 243 1.59e-25 97.8
Msa0892380 MtrunA17_Chr6g0474671 49.515 103 49 2 5 104 71 173 2.63e-25 97.1
Msa0892380 MtrunA17_Chr1g0204361 43.750 112 55 4 3 109 17 125 1.29e-22 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0892380 AT5G51780.1 46.364 110 53 2 5 109 2 110 5.88e-26 97.4
Msa0892380 AT5G51780.2 46.364 110 53 2 5 109 25 133 1.65e-25 97.1
Msa0892380 AT4G25410.1 40.678 118 65 3 9 121 47 164 3.89e-23 91.3
Msa0892380 AT4G25400.2 45.455 110 55 3 2 109 21 127 1.08e-22 89.4
Msa0892380 AT4G25400.1 44.037 109 58 2 2 109 21 127 1.12e-22 89.4
Msa0892380 AT5G51790.4 43.548 124 65 4 2 121 24 146 1.48e-21 86.7
Msa0892380 AT5G51790.1 43.548 124 65 4 2 121 24 146 2.11e-21 86.3
Msa0892380 AT5G51790.2 43.548 124 65 4 2 121 89 211 6.46e-21 86.3
Msa0892380 AT5G51790.3 43.548 124 65 4 2 121 89 211 6.89e-21 86.3
Msa0892380 AT1G12540.1 43.651 126 57 3 3 116 73 196 7.23e-21 86.3
Msa0892380 AT1G62975.2 49.398 83 40 1 3 85 72 152 3.01e-20 84.3
Msa0892380 AT1G62975.1 49.398 83 40 1 3 85 72 152 4.75e-20 84.0

Find 49 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGAAATTGAAGTATATT+TGG 0.176459 6_1:-72127322 None:intergenic
CTCAAGATATTGTCGATTTC+AGG 0.181886 6_1:-72131361 None:intergenic
GTTTCACCACTTGTGGGATT+TGG 0.215430 6_1:+72131638 Msa0892380:three_prime_UTR
TTGAAGTATATTTGGTTGTT+AGG 0.250422 6_1:-72127314 None:intergenic
TTTCACCACTTGTGGGATTT+GGG 0.284053 6_1:+72131639 Msa0892380:three_prime_UTR
ATTTGGGAGACAAAGATTAT+AGG 0.305099 6_1:+72127556 Msa0892380:five_prime_UTR
TCTTCCTATAGAATATATTA+AGG 0.316735 6_1:+72127679 Msa0892380:CDS
TTACATTTGCCAGATATGTT+TGG 0.363977 6_1:-72127278 None:intergenic
TAATCAATTCCAGTCATTAT+CGG 0.381800 6_1:-72131170 None:intergenic
TCTGAGAATCCAAACATATC+TGG 0.381962 6_1:+72127269 Msa0892380:five_prime_UTR
TGAAGTATATTTGGTTGTTA+GGG 0.384471 6_1:-72127313 None:intergenic
ATTATAGGATATTGTTATCA+TGG 0.391937 6_1:+72127571 Msa0892380:exon
ATGTCTAACACTCTTGATAA+AGG 0.395077 6_1:-72128923 None:intergenic
TTTCCAAGGTTGTATTTCAA+TGG 0.407577 6_1:-72127465 None:intergenic
CTATAGGAAGAAGCGATCTA+AGG 0.415824 6_1:-72127667 None:intergenic
TCATGGAAGAAAACAAGAAA+TGG 0.438064 6_1:+72127588 Msa0892380:exon
ATCTCAAGTCCTCCAGGAAA+AGG 0.460588 6_1:-72128866 None:intergenic
AATGAGGTTTCACCACTTGT+GGG 0.464388 6_1:+72131632 Msa0892380:three_prime_UTR
ACTTGAGCATGATATTGCTT+CGG 0.469019 6_1:-72131216 None:intergenic
AGAGCGTATACTATCTTTGT+GGG 0.473483 6_1:-72127364 None:intergenic
CTAACCTTAATATATTCTAT+AGG 0.474465 6_1:-72127683 None:intergenic
GAATGAGGTTTCACCACTTG+TGG 0.479464 6_1:+72131631 Msa0892380:three_prime_UTR
GAAGCAATTACAAGATAAGA+AGG 0.481546 6_1:+72128751 Msa0892380:CDS
GAGAGCGTATACTATCTTTG+TGG 0.506519 6_1:-72127365 None:intergenic
TAAGAAGGAAGAGTTAATGA+AGG 0.514489 6_1:+72128766 Msa0892380:CDS
TGATGATCTTCTAATTTCCA+AGG 0.516391 6_1:-72127479 None:intergenic
TTGAAGATTCAAATTCATAG+TGG 0.521469 6_1:+72131432 Msa0892380:three_prime_UTR
CACACAATCTCAAGTCCTCC+AGG 0.527587 6_1:-72128872 None:intergenic
AATTTCTGATCATGTGAATG+AGG 0.530841 6_1:+72128694 Msa0892380:CDS
CATGATACTTGTGAGTCAAA+TGG 0.531228 6_1:+72127509 Msa0892380:five_prime_UTR
TTGAAGGAACCGATAATGAC+TGG 0.537483 6_1:+72131161 Msa0892380:intron
TCTAAGGTTGGTGCAGAGGT+TGG 0.539114 6_1:-72127651 None:intergenic
TGCATGTGTGGACTTGAATG+AGG 0.542338 6_1:+72131616 Msa0892380:three_prime_UTR
TTTGTTGTAGTTAGAAACAA+TGG 0.544147 6_1:+72131492 Msa0892380:three_prime_UTR
AAAGGTTGAACAATAACAAA+GGG 0.545126 6_1:-72128848 None:intergenic
GCGATCTAAGGTTGGTGCAG+AGG 0.557780 6_1:-72127655 None:intergenic
AAGAGACAGAAAGACAAAGA+AGG 0.564940 6_1:+72127618 Msa0892380:CDS
TGATCTTATCTTATATGTGA+AGG 0.566527 6_1:+72127244 None:intergenic
CTTGTGAGTCAAATGGTGAA+GGG 0.569536 6_1:+72127516 Msa0892380:five_prime_UTR
CCAAGGTTGTATTTCAATGG+TGG 0.574419 6_1:-72127462 None:intergenic
CCACCATTGAAATACAACCT+TGG 0.577393 6_1:+72127462 Msa0892380:five_prime_UTR
ACAAATGCCACTACTCAAGA+TGG 0.579156 6_1:+72128803 Msa0892380:CDS
AGGAAGAAGCGATCTAAGGT+TGG 0.595316 6_1:-72127663 None:intergenic
AAAAGGTTGAACAATAACAA+AGG 0.600268 6_1:-72128849 None:intergenic
ACTTGTGAGTCAAATGGTGA+AGG 0.611143 6_1:+72127515 Msa0892380:five_prime_UTR
CTGAACCCAAATCCCACAAG+TGG 0.621804 6_1:-72131644 None:intergenic
ACTAGAGCCATCTTGAGTAG+TGG 0.645479 6_1:-72128810 None:intergenic
TTGACAAAGATACTATACAA+AGG 0.681733 6_1:-72127340 None:intergenic
GAGCGTATACTATCTTTGTG+GGG 0.690864 6_1:-72127363 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGTAGAATTTTTATTATT+TGG + chr6_1:72127539-72127558 Msa0892380:five_prime_UTR 10.0%
!!! AAGTAGAATTTTTATTATTT+GGG + chr6_1:72127540-72127559 Msa0892380:five_prime_UTR 10.0%
!!! TGGAAATATTTAATTTTTTA+TGG + chr6_1:72129341-72129360 Msa0892380:intron 10.0%
!! AAAATTAATAATCTCAAAAT+AGG + chr6_1:72130100-72130119 Msa0892380:intron 10.0%
!! AAAATTAAAAATACAAAAGA+AGG - chr6_1:72130621-72130640 None:intergenic 10.0%
!!! TAATATATTATCTTTTTTGT+TGG + chr6_1:72130682-72130701 Msa0892380:intron 10.0%
!! ACAAGAAAATAAATAATCTT+GGG - chr6_1:72128568-72128587 None:intergenic 15.0%
!! AACAAGAAAATAAATAATCT+TGG - chr6_1:72128569-72128588 None:intergenic 15.0%
!!! TTTATTTGAAATTTGAAATC+TGG + chr6_1:72128601-72128620 Msa0892380:intron 15.0%
!! TAGAAAAAAAAAATAATGCA+TGG - chr6_1:72129060-72129079 None:intergenic 15.0%
!! TATAATAGTTGCAATATTTA+AGG + chr6_1:72129231-72129250 Msa0892380:intron 15.0%
!! TATGGAAATATTTCTTATTA+TGG + chr6_1:72129359-72129378 Msa0892380:intron 15.0%
!! ATCTATAAAACTAAACAATT+AGG - chr6_1:72130362-72130381 None:intergenic 15.0%
!!! GTATTTTTAATTTTTTGTGT+CGG + chr6_1:72130627-72130646 Msa0892380:intron 15.0%
!!! ACTTATTTTTGCTATATATA+AGG - chr6_1:72130912-72130931 None:intergenic 15.0%
!! TGTTAATTGGTTAATTTATT+AGG - chr6_1:72131070-72131089 None:intergenic 15.0%
!! TAATCAAATTTACAACATAT+GGG - chr6_1:72131121-72131140 None:intergenic 15.0%
!! AAAGGAAATTGAAGTATATT+TGG - chr6_1:72127325-72127344 None:intergenic 20.0%
!!! TTTTAATTATTTGGCTATAG+AGG - chr6_1:72127397-72127416 None:intergenic 20.0%
!!! GAACTCAACTTTTAATTATT+TGG - chr6_1:72127406-72127425 None:intergenic 20.0%
!! ATTATAGGATATTGTTATCA+TGG + chr6_1:72127571-72127590 Msa0892380:exon 20.0%
!! TCTTCCTATAGAATATATTA+AGG + chr6_1:72127679-72127698 Msa0892380:CDS 20.0%
!! CTAACCTTAATATATTCTAT+AGG - chr6_1:72127686-72127705 None:intergenic 20.0%
!! ATATGAATCTCATTTAAATG+TGG - chr6_1:72127894-72127913 None:intergenic 20.0%
!!! TACGTTTATACTCTTTTATT+AGG - chr6_1:72127990-72128009 None:intergenic 20.0%
!!! AACAACAAGGTTTTATAAAT+CGG - chr6_1:72128101-72128120 None:intergenic 20.0%
!!! GTAACATAATGTTTTCTTAT+AGG - chr6_1:72128293-72128312 None:intergenic 20.0%
!! AAGAAAATAAATAATCTTGG+GGG - chr6_1:72128566-72128585 None:intergenic 20.0%
!! CAAGAAAATAAATAATCTTG+GGG - chr6_1:72128567-72128586 None:intergenic 20.0%
!!! GCATTATTTTTTTTTTCTAG+TGG + chr6_1:72129061-72129080 Msa0892380:intron 20.0%
!!! CATAAATAGTTGTTACTTAA+AGG - chr6_1:72129293-72129312 None:intergenic 20.0%
!! ATTTCTCTTATAAATAGGAT+CGG - chr6_1:72129617-72129636 None:intergenic 20.0%
!! TCCTATTTATAAGAGAAATT+TGG + chr6_1:72129618-72129637 Msa0892380:intron 20.0%
!! CCTATTTATAAGAGAAATTT+GGG + chr6_1:72129619-72129638 Msa0892380:intron 20.0%
!! TAGAAGTTTACATTATTACA+AGG - chr6_1:72129748-72129767 None:intergenic 20.0%
!! GTAATAATGTAAACTTCTAA+TGG + chr6_1:72129749-72129768 Msa0892380:intron 20.0%
!!! GAAAATTAACCAATTTGTTA+AGG + chr6_1:72129858-72129877 Msa0892380:intron 20.0%
!!! CATCTATAAGGATTTTTTTA+TGG - chr6_1:72130162-72130181 None:intergenic 20.0%
!! TAAAAAAATCCTTATAGATG+AGG + chr6_1:72130162-72130181 Msa0892380:intron 20.0%
!! TATCAAATGATGAATTGTTT+AGG - chr6_1:72130319-72130338 None:intergenic 20.0%
!!! ATTGTTTAGTTTTATAGATG+AGG + chr6_1:72130363-72130382 Msa0892380:intron 20.0%
!! TAAAAGTTATTCCTTAGAAA+TGG + chr6_1:72130400-72130419 Msa0892380:intron 20.0%
!! AGGAAGTGAATAAAATATTA+TGG - chr6_1:72130601-72130620 None:intergenic 20.0%
!!! TTTTTTATTACATAGTCTAG+TGG + chr6_1:72130780-72130799 Msa0892380:intron 20.0%
!!! CTCATTTTTGCTTATAATTA+AGG + chr6_1:72130990-72131009 Msa0892380:intron 20.0%
!! ATAATTAAGGAAGAAGTAAA+AGG + chr6_1:72131003-72131022 Msa0892380:intron 20.0%
!! TAATTAAGGAAGAAGTAAAA+GGG + chr6_1:72131004-72131023 Msa0892380:intron 20.0%
!! GTAATCAAATTTACAACATA+TGG - chr6_1:72131122-72131141 None:intergenic 20.0%
!!! TATTTTATTTTTTGCATGTG+TGG + chr6_1:72131604-72131623 Msa0892380:three_prime_UTR 20.0%
! TGAAGTATATTTGGTTGTTA+GGG - chr6_1:72127316-72127335 None:intergenic 25.0%
! TTGAAGTATATTTGGTTGTT+AGG - chr6_1:72127317-72127336 None:intergenic 25.0%
! TTGACAAAGATACTATACAA+AGG - chr6_1:72127343-72127362 None:intergenic 25.0%
!!! TCTTGATAGGATTGTTATTT+TGG - chr6_1:72127859-72127878 None:intergenic 25.0%
! ATATTGAAAGAAATGCGTAT+GGG + chr6_1:72127910-72127929 Msa0892380:intron 25.0%
! GTAATGTTACACTTTAGATA+TGG - chr6_1:72127957-72127976 None:intergenic 25.0%
!!! ACAACAAGGTTTTATAAATC+GGG - chr6_1:72128100-72128119 None:intergenic 25.0%
! ATAATATGAATTGTCAGTAG+CGG - chr6_1:72128267-72128286 None:intergenic 25.0%
!! GAAATTTGAAATCTGGTATA+TGG + chr6_1:72128608-72128627 Msa0892380:intron 25.0%
! GGTATATGGTTAGTATTAAT+CGG + chr6_1:72128622-72128641 Msa0892380:intron 25.0%
! GTATATGGTTAGTATTAATC+GGG + chr6_1:72128623-72128642 Msa0892380:intron 25.0%
! TATATGGTTAGTATTAATCG+GGG + chr6_1:72128624-72128643 Msa0892380:intron 25.0%
! TGATTAATGTCATCATGATT+AGG + chr6_1:72128661-72128680 Msa0892380:intron 25.0%
! GATTAATGTCATCATGATTA+GGG + chr6_1:72128662-72128681 Msa0892380:intron 25.0%
!!! ATTGCTTCACTTTATTTTGT+AGG - chr6_1:72128739-72128758 None:intergenic 25.0%
! AAAGGTTGAACAATAACAAA+GGG - chr6_1:72128851-72128870 None:intergenic 25.0%
! AAAAGGTTGAACAATAACAA+AGG - chr6_1:72128852-72128871 None:intergenic 25.0%
! ACAATGCATTATTATATGCA+GGG - chr6_1:72129142-72129161 None:intergenic 25.0%
! TAAGCATAATTAATACTCCA+TGG - chr6_1:72129195-72129214 None:intergenic 25.0%
! TTTATGAAAAATTGTGCGAT+CGG + chr6_1:72129307-72129326 Msa0892380:intron 25.0%
!!! TTTGTTATTAGAGATGTGTT+TGG + chr6_1:72129504-72129523 Msa0892380:intron 25.0%
! CCCAAATTTCTCTTATAAAT+AGG - chr6_1:72129622-72129641 None:intergenic 25.0%
! TCAACAAAAGTGAATGTATT+TGG - chr6_1:72129681-72129700 None:intergenic 25.0%
! CCTATTTATAAGAGAAAGTT+AGG - chr6_1:72129704-72129723 None:intergenic 25.0%
! CCTAACTTTCTCTTATAAAT+AGG + chr6_1:72129701-72129720 Msa0892380:intron 25.0%
! TAAACTTCTAATGGTCTATA+TGG + chr6_1:72129758-72129777 Msa0892380:intron 25.0%
!! GTATCATATAGAACATGTTT+TGG + chr6_1:72129897-72129916 Msa0892380:intron 25.0%
! AAAAAACATCCTCATCTATA+AGG - chr6_1:72130174-72130193 None:intergenic 25.0%
! GTCATAGTTTATATAATGAG+TGG + chr6_1:72130445-72130464 Msa0892380:intron 25.0%
!!! GTTTTTTAATGCAAGTACAA+GGG - chr6_1:72130529-72130548 None:intergenic 25.0%
!!! TGTTTTTTAATGCAAGTACA+AGG - chr6_1:72130530-72130549 None:intergenic 25.0%
! AGAAAGATTTGTAATTGCAA+TGG + chr6_1:72130715-72130734 Msa0892380:intron 25.0%
! GACATTGCATAATATATACA+GGG - chr6_1:72130857-72130876 None:intergenic 25.0%
! AGACATTGCATAATATATAC+AGG - chr6_1:72130858-72130877 None:intergenic 25.0%
!! TTTAGAGTTTGATGTTTGAT+AGG + chr6_1:72131027-72131046 Msa0892380:intron 25.0%
! ATATGGACAAAACTGTTAAT+TGG - chr6_1:72131083-72131102 None:intergenic 25.0%
!! GGATACTCTTTTATTACATA+TGG - chr6_1:72131100-72131119 None:intergenic 25.0%
! TAATCAATTCCAGTCATTAT+CGG - chr6_1:72131173-72131192 None:intergenic 25.0%
!! ATTGAATTGAATCATTTTGC+AGG + chr6_1:72131316-72131335 Msa0892380:intron 25.0%
! TTGAAGATTCAAATTCATAG+TGG + chr6_1:72131432-72131451 Msa0892380:three_prime_UTR 25.0%
!! TTTGTTGTAGTTAGAAACAA+TGG + chr6_1:72131492-72131511 Msa0892380:three_prime_UTR 25.0%
TTACATTTGCCAGATATGTT+TGG - chr6_1:72127281-72127300 None:intergenic 30.0%
!! GAGTTCGTCTTCTTTTATTT+TGG + chr6_1:72127420-72127439 Msa0892380:five_prime_UTR 30.0%
TTTCCAAGGTTGTATTTCAA+TGG - chr6_1:72127468-72127487 None:intergenic 30.0%
TGATGATCTTCTAATTTCCA+AGG - chr6_1:72127482-72127501 None:intergenic 30.0%
ATTTGGGAGACAAAGATTAT+AGG + chr6_1:72127556-72127575 Msa0892380:five_prime_UTR 30.0%
TCATGGAAGAAAACAAGAAA+TGG + chr6_1:72127588-72127607 Msa0892380:exon 30.0%
ATAAATTCACATGTCTCCTA+AGG + chr6_1:72127760-72127779 Msa0892380:intron 30.0%
GCAACACTTTAAATCTTGAT+AGG - chr6_1:72127872-72127891 None:intergenic 30.0%
CATATTGAAAGAAATGCGTA+TGG + chr6_1:72127909-72127928 Msa0892380:intron 30.0%
GATATGGATCACATATAAAC+TGG - chr6_1:72127941-72127960 None:intergenic 30.0%
TTCTCTTATCGACAATATCA+AGG + chr6_1:72128044-72128063 Msa0892380:intron 30.0%
AAAGCTAAACTCATACCATT+GGG - chr6_1:72128207-72128226 None:intergenic 30.0%
AAAAGCTAAACTCATACCAT+TGG - chr6_1:72128208-72128227 None:intergenic 30.0%
AATAAATAATCTTGGGGGAT+GGG - chr6_1:72128561-72128580 None:intergenic 30.0%
AAATAAATAATCTTGGGGGA+TGG - chr6_1:72128562-72128581 None:intergenic 30.0%
AATTTCTGATCATGTGAATG+AGG + chr6_1:72128694-72128713 Msa0892380:CDS 30.0%
GAAGCAATTACAAGATAAGA+AGG + chr6_1:72128751-72128770 Msa0892380:CDS 30.0%
TAAGAAGGAAGAGTTAATGA+AGG + chr6_1:72128766-72128785 Msa0892380:CDS 30.0%
ATGTCTAACACTCTTGATAA+AGG - chr6_1:72128926-72128945 None:intergenic 30.0%
CATATACACTATAAGATCTG+AGG + chr6_1:72129006-72129025 Msa0892380:intron 30.0%
ACCTTATAAGGTGAATAAGT+GGG + chr6_1:72129093-72129112 Msa0892380:intron 30.0%
CAATGCATTATTATATGCAG+GGG - chr6_1:72129141-72129160 None:intergenic 30.0%
GACAATGCATTATTATATGC+AGG - chr6_1:72129143-72129162 None:intergenic 30.0%
CTTTCTCTTATAAATAGGAC+CGG + chr6_1:72129706-72129725 Msa0892380:intron 30.0%
!! TACTACATGGATTACTGTTT+TGG + chr6_1:72129790-72129809 Msa0892380:intron 30.0%
!!! AGATGAGGATGTTTTTTTTG+TGG + chr6_1:72130177-72130196 Msa0892380:intron 30.0%
CTAATAAGGAACCATTTCTA+AGG - chr6_1:72130414-72130433 None:intergenic 30.0%
CTTAGAAATGGTTCCTTATT+AGG + chr6_1:72130412-72130431 Msa0892380:intron 30.0%
!! GACTCTCATTTTTCCTAATA+AGG - chr6_1:72130428-72130447 None:intergenic 30.0%
GAGAATATTACAAGTTGTCT+GGG - chr6_1:72130478-72130497 None:intergenic 30.0%
TGAGAATATTACAAGTTGTC+TGG - chr6_1:72130479-72130498 None:intergenic 30.0%
TAAAAAACACTGTACTCAGA+AGG + chr6_1:72130541-72130560 Msa0892380:intron 30.0%
AAAAAACACTGTACTCAGAA+GGG + chr6_1:72130542-72130561 Msa0892380:intron 30.0%
AGAAAGGTAGTACAAACATA+TGG - chr6_1:72130747-72130766 None:intergenic 30.0%
AAAAAAAAAAGGACGCAGAA+AGG - chr6_1:72130763-72130782 None:intergenic 30.0%
CATTCAATTTATTGCCTTGA+AGG + chr6_1:72131145-72131164 Msa0892380:intron 30.0%
TCTGAGAATCCAAACATATC+TGG + chr6_1:72127269-72127288 Msa0892380:five_prime_UTR 35.0%
AGAGCGTATACTATCTTTGT+GGG - chr6_1:72127367-72127386 None:intergenic 35.0%
CATGATACTTGTGAGTCAAA+TGG + chr6_1:72127509-72127528 Msa0892380:five_prime_UTR 35.0%
AAGAGACAGAAAGACAAAGA+AGG + chr6_1:72127618-72127637 Msa0892380:CDS 35.0%
AACAACAATATTGCCTCCTT+AGG - chr6_1:72127779-72127798 None:intergenic 35.0%
! GGAGGCAATATTGTTGTTAA+CGG + chr6_1:72127781-72127800 Msa0892380:intron 35.0%
AAACTCAGCATCAAACAACA+AGG - chr6_1:72128114-72128133 None:intergenic 35.0%
!!! GGTATGAGTTTAGCTTTTGA+AGG + chr6_1:72128210-72128229 Msa0892380:intron 35.0%
! TTGAAGGAGTACGAATAAAG+AGG + chr6_1:72128226-72128245 Msa0892380:intron 35.0%
TGAACTAAGGGATTAAAGGA+GGG - chr6_1:72128521-72128540 None:intergenic 35.0%
TTGAACTAAGGGATTAAAGG+AGG - chr6_1:72128522-72128541 None:intergenic 35.0%
TGGTTGAACTAAGGGATTAA+AGG - chr6_1:72128525-72128544 None:intergenic 35.0%
AACTCACATGGTTGAACTAA+GGG - chr6_1:72128533-72128552 None:intergenic 35.0%
TAACTCACATGGTTGAACTA+AGG - chr6_1:72128534-72128553 None:intergenic 35.0%
!!! GCTTCACTTTATTTTGTAGG+TGG - chr6_1:72128736-72128755 None:intergenic 35.0%
! GTTATTGTTCAACCTTTTCC+TGG + chr6_1:72128854-72128873 Msa0892380:CDS 35.0%
! TTAGACATGCTGCTTAAAGA+AGG + chr6_1:72128938-72128957 Msa0892380:intron 35.0%
! TAGACATGCTGCTTAAAGAA+GGG + chr6_1:72128939-72128958 Msa0892380:intron 35.0%
TGTACTTCCAACATGATAGA+AGG + chr6_1:72128977-72128996 Msa0892380:intron 35.0%
GAATCTTCCTTCTATCATGT+TGG - chr6_1:72128987-72129006 None:intergenic 35.0%
TGGCTAGAATTCACCTTATA+AGG + chr6_1:72129081-72129100 Msa0892380:intron 35.0%
CACCTTATAAGGTGAATAAG+TGG + chr6_1:72129092-72129111 Msa0892380:intron 35.0%
CCTTATAAGGTGAATAAGTG+GGG + chr6_1:72129094-72129113 Msa0892380:intron 35.0%
GCATAATTAATACTCCATGG+TGG - chr6_1:72129192-72129211 None:intergenic 35.0%
AAATTGTGCGATCGGTATAT+TGG + chr6_1:72129315-72129334 Msa0892380:intron 35.0%
ATGAACGTGTGAAATGAGAA+AGG - chr6_1:72129468-72129487 None:intergenic 35.0%
AAGCTGTAGTCTGATGATAT+GGG + chr6_1:72129535-72129554 Msa0892380:intron 35.0%
! AGTCTGATGATATGGGTTAT+TGG + chr6_1:72129542-72129561 Msa0892380:intron 35.0%
! GTCTGATGATATGGGTTATT+GGG + chr6_1:72129543-72129562 Msa0892380:intron 35.0%
GGGACATTGAATGAGTATTA+AGG + chr6_1:72129563-72129582 Msa0892380:intron 35.0%
CTCTTATAAATAGGATCGGA+GGG - chr6_1:72129613-72129632 None:intergenic 35.0%
TCTCTTATAAATAGGATCGG+AGG - chr6_1:72129614-72129633 None:intergenic 35.0%
CTCACAAGTCCTTAACAAAT+TGG - chr6_1:72129870-72129889 None:intergenic 35.0%
!!! ATCGTCTAGTTTTCAAGAGA+AGG + chr6_1:72130202-72130221 Msa0892380:intron 35.0%
!!! TCGTCTAGTTTTCAAGAGAA+GGG + chr6_1:72130203-72130222 Msa0892380:intron 35.0%
CAACAGAACTCTAATAACTC+TGG - chr6_1:72130240-72130259 None:intergenic 35.0%
GTTATTAGAGTTCTGTTGAG+AGG + chr6_1:72130242-72130261 Msa0892380:intron 35.0%
!! CTAGTGGCTTCGTTTTTATA+CGG + chr6_1:72130796-72130815 Msa0892380:intron 35.0%
TGCATAATATATACAGGGTC+TGG - chr6_1:72130852-72130871 None:intergenic 35.0%
ACTTGAGCATGATATTGCTT+CGG - chr6_1:72131219-72131238 None:intergenic 35.0%
!! CTCAAGATATTGTCGATTTC+AGG - chr6_1:72131364-72131383 None:intergenic 35.0%
AAAAAAGATCAACGAGCTCA+TGG - chr6_1:72131473-72131492 None:intergenic 35.0%
GAGCGTATACTATCTTTGTG+GGG - chr6_1:72127366-72127385 None:intergenic 40.0%
! GAGAGCGTATACTATCTTTG+TGG - chr6_1:72127368-72127387 None:intergenic 40.0%
CCAAGGTTGTATTTCAATGG+TGG - chr6_1:72127465-72127484 None:intergenic 40.0%
CCACCATTGAAATACAACCT+TGG + chr6_1:72127462-72127481 Msa0892380:five_prime_UTR 40.0%
ACTTGTGAGTCAAATGGTGA+AGG + chr6_1:72127515-72127534 Msa0892380:five_prime_UTR 40.0%
CTTGTGAGTCAAATGGTGAA+GGG + chr6_1:72127516-72127535 Msa0892380:five_prime_UTR 40.0%
CTATAGGAAGAAGCGATCTA+AGG - chr6_1:72127670-72127689 None:intergenic 40.0%
AATTCACATGTCTCCTAAGG+AGG + chr6_1:72127763-72127782 Msa0892380:intron 40.0%
AGGGAAGGAATAAAGATCCA+GGG - chr6_1:72128502-72128521 None:intergenic 40.0%
ACAAATGCCACTACTCAAGA+TGG + chr6_1:72128803-72128822 Msa0892380:CDS 40.0%
! ATTGTTCAACCTTTTCCTGG+AGG + chr6_1:72128857-72128876 Msa0892380:CDS 40.0%
CCCCACTTATTCACCTTATA+AGG - chr6_1:72129097-72129116 None:intergenic 40.0%
!! TGCCTCAAGAGCATTCTTTA+AGG + chr6_1:72129267-72129286 Msa0892380:intron 40.0%
TGCGATCGGTATATTGGAAA+TGG + chr6_1:72129321-72129340 Msa0892380:intron 40.0%
CAAGCTGTAGTCTGATGATA+TGG + chr6_1:72129534-72129553 Msa0892380:intron 40.0%
TCTCTTATAAATAGGACCGG+AGG + chr6_1:72129709-72129728 Msa0892380:intron 40.0%
CTCTTATAAATAGGACCGGA+GGG + chr6_1:72129710-72129729 Msa0892380:intron 40.0%
AGGAAAACATATACTCCCTC+CGG - chr6_1:72129728-72129747 None:intergenic 40.0%
ATGGCATGTCTTGTACTACA+TGG + chr6_1:72129777-72129796 Msa0892380:intron 40.0%
! ATCAGCTTTGACAAATCCAG+TGG - chr6_1:72129954-72129973 None:intergenic 40.0%
AATCTGAGTTCGATTCTTGG+TGG - chr6_1:72130294-72130313 None:intergenic 40.0%
GAGAATCTGAGTTCGATTCT+TGG - chr6_1:72130297-72130316 None:intergenic 40.0%
ATATTCTCATGACCGTCTAG+TGG + chr6_1:72130490-72130509 Msa0892380:intron 40.0%
TATTCTCATGACCGTCTAGT+GGG + chr6_1:72130491-72130510 Msa0892380:intron 40.0%
! AAGTACAAGGGTACACTAAC+CGG - chr6_1:72130517-72130536 None:intergenic 40.0%
GTGAATAAGTGACGTGTTTG+AGG + chr6_1:72130818-72130837 Msa0892380:intron 40.0%
!! AGTCATTATCGGTTCCTTCA+AGG - chr6_1:72131162-72131181 None:intergenic 40.0%
TTGAAGGAACCGATAATGAC+TGG + chr6_1:72131161-72131180 Msa0892380:intron 40.0%
AATGAGGTTTCACCACTTGT+GGG + chr6_1:72131632-72131651 Msa0892380:three_prime_UTR 40.0%
! AGGAAGAAGCGATCTAAGGT+TGG - chr6_1:72127666-72127685 None:intergenic 45.0%
CTTTGACAAGATTCGAACCC+TGG + chr6_1:72128482-72128501 Msa0892380:intron 45.0%
GAGGGAAGGAATAAAGATCC+AGG - chr6_1:72128503-72128522 None:intergenic 45.0%
CTAAGGGATTAAAGGAGGGA+AGG - chr6_1:72128517-72128536 None:intergenic 45.0%
ACTAGAGCCATCTTGAGTAG+TGG - chr6_1:72128813-72128832 None:intergenic 45.0%
ATCTCAAGTCCTCCAGGAAA+AGG - chr6_1:72128869-72128888 None:intergenic 45.0%
ATTATTATATGCAGGGGCCG+AGG - chr6_1:72129135-72129154 None:intergenic 45.0%
TACCAACTGAGCTATGCTCA+CGG + chr6_1:72129164-72129183 Msa0892380:intron 45.0%
GGCCTTAAAGAATGCTCTTG+AGG - chr6_1:72129272-72129291 None:intergenic 45.0%
TGGAATCCTTCAAGAGTCTC+CGG + chr6_1:72129379-72129398 Msa0892380:intron 45.0%
GCTTTGACAAATCCAGTGGA+GGG - chr6_1:72129950-72129969 None:intergenic 45.0%
! AGCTTTGACAAATCCAGTGG+AGG - chr6_1:72129951-72129970 None:intergenic 45.0%
TGCATGTGTGGACTTGAATG+AGG + chr6_1:72131616-72131635 Msa0892380:three_prime_UTR 45.0%
GAATGAGGTTTCACCACTTG+TGG + chr6_1:72131631-72131650 Msa0892380:three_prime_UTR 45.0%
!! AAAAAGATAATATATTAATA+AGG - chr6_1:72130679-72130698 None:intergenic 5.0%
!! TATGTAATAAAAAAAAAAAA+AGG - chr6_1:72130774-72130793 None:intergenic 5.0%
!! TCTAAGGTTGGTGCAGAGGT+TGG - chr6_1:72127654-72127673 None:intergenic 50.0%
GGATGGGTACGTAACTCACA+TGG - chr6_1:72128545-72128564 None:intergenic 50.0%
CACACAATCTCAAGTCCTCC+AGG - chr6_1:72128875-72128894 None:intergenic 50.0%
! AGGTGAATAAGTGGGGTGTC+CGG + chr6_1:72129101-72129120 Msa0892380:intron 50.0%
GTGAGCATAGCTCAGTTGGT+AGG - chr6_1:72129165-72129184 None:intergenic 50.0%
ACCAACTGAGCTATGCTCAC+GGG + chr6_1:72129165-72129184 Msa0892380:intron 50.0%
GGAATCCTTCAAGAGTCTCC+GGG + chr6_1:72129380-72129399 Msa0892380:intron 50.0%
GAATCCTTCAAGAGTCTCCG+GGG + chr6_1:72129381-72129400 Msa0892380:intron 50.0%
AATCCTTCAAGAGTCTCCGG+GGG + chr6_1:72129382-72129401 Msa0892380:intron 50.0%
GTCATAATAACGAGTGCCCC+CGG - chr6_1:72129401-72129420 None:intergenic 50.0%
GGGTGGCGACAGATATTTAG+TGG - chr6_1:72129930-72129949 None:intergenic 50.0%
TTGACAAATCCAGTGGAGGG+TGG - chr6_1:72129947-72129966 None:intergenic 50.0%
ACACTGTACTCAGAAGGGCT+TGG + chr6_1:72130547-72130566 Msa0892380:intron 50.0%
CACTGTACTCAGAAGGGCTT+GGG + chr6_1:72130548-72130567 Msa0892380:intron 50.0%
!! GCGATCTAAGGTTGGTGCAG+AGG - chr6_1:72127658-72127677 None:intergenic 55.0%
TCGTCGAAGATGCAGCCCAA+TGG + chr6_1:72128189-72128208 Msa0892380:intron 55.0%
! GGTGAATAAGTGGGGTGTCC+GGG + chr6_1:72129102-72129121 Msa0892380:intron 55.0%
! GTGAATAAGTGGGGTGTCCG+GGG + chr6_1:72129103-72129122 Msa0892380:intron 55.0%
TCCCGTGAGCATAGCTCAGT+TGG - chr6_1:72129169-72129188 None:intergenic 55.0%
ACACTAACCGGCCCACTAGA+CGG - chr6_1:72130505-72130524 None:intergenic 55.0%
ACTCAGAAGGGCTTGGGTGT+TGG + chr6_1:72130554-72130573 Msa0892380:intron 55.0%
CTATGCTCACGGGACCACCA+TGG + chr6_1:72129175-72129194 Msa0892380:intron 60.0%
TATCTGTCGCCACCCTCCAC+TGG + chr6_1:72129935-72129954 Msa0892380:intron 60.0%
! CTCATGACCGTCTAGTGGGC+CGG + chr6_1:72130495-72130514 Msa0892380:intron 60.0%
!! CAGAAGGGCTTGGGTGTTGG+TGG + chr6_1:72130557-72130576 Msa0892380:intron 60.0%
!! GGCTTGGGTGTTGGTGGTGT+GGG + chr6_1:72130563-72130582 Msa0892380:intron 60.0%
!! TTGGGTGTTGGTGGTGTGGG+TGG + chr6_1:72130566-72130585 Msa0892380:intron 60.0%
!! GTTGGTGGTGTGGGTGGCTT+AGG + chr6_1:72130572-72130591 Msa0892380:intron 60.0%
GGTGTCCGGGGTTCGAACCT+CGG + chr6_1:72129115-72129134 Msa0892380:intron 65.0%
GTGCCCCCGGAGACTCTTGA+AGG - chr6_1:72129388-72129407 None:intergenic 65.0%
!! GGGCTTGGGTGTTGGTGGTG+TGG + chr6_1:72130562-72130581 Msa0892380:intron 65.0%
AGGGGCCGAGGTTCGAACCC+CGG - chr6_1:72129123-72129142 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 72127245 72131660 72127245 ID=Msa0892380;Name=Msa0892380
chr6_1 mRNA 72127245 72131660 72127245 ID=Msa0892380-mRNA-1;Parent=Msa0892380;Name=Msa0892380-mRNA-1;_AED=0.05;_eAED=0.05;_QI=345|0.66|0.75|1|1|1|4|273|160
chr6_1 exon 72127245 72127700 72127245 ID=Msa0892380-mRNA-1:exon:12351;Parent=Msa0892380-mRNA-1
chr6_1 exon 72128683 72128934 72128683 ID=Msa0892380-mRNA-1:exon:12352;Parent=Msa0892380-mRNA-1
chr6_1 exon 72131167 72131236 72131167 ID=Msa0892380-mRNA-1:exon:12353;Parent=Msa0892380-mRNA-1
chr6_1 exon 72131338 72131660 72131338 ID=Msa0892380-mRNA-1:exon:12354;Parent=Msa0892380-mRNA-1
chr6_1 five_prime_UTR 72127245 72127589 72127245 ID=Msa0892380-mRNA-1:five_prime_utr;Parent=Msa0892380-mRNA-1
chr6_1 CDS 72127590 72127700 72127590 ID=Msa0892380-mRNA-1:cds;Parent=Msa0892380-mRNA-1
chr6_1 CDS 72128683 72128934 72128683 ID=Msa0892380-mRNA-1:cds;Parent=Msa0892380-mRNA-1
chr6_1 CDS 72131167 72131236 72131167 ID=Msa0892380-mRNA-1:cds;Parent=Msa0892380-mRNA-1
chr6_1 CDS 72131338 72131387 72131338 ID=Msa0892380-mRNA-1:cds;Parent=Msa0892380-mRNA-1
chr6_1 three_prime_UTR 72131388 72131660 72131388 ID=Msa0892380-mRNA-1:three_prime_utr;Parent=Msa0892380-mRNA-1
Gene Sequence

>Msa0892380

ATGGAAGAAAACAAGAAATGGATGCATAAAGAGACAGAAAGACAAAGAAGGCAAGAGATGACCAACCTCTGCACCAACCTTAGATCGCTTCTTCCTATAGAATATATTAAGGGAAAACGCTCAATTTCTGATCATGTGAATGAGGCTACGAATTATATCAACCACCTACAAAATAAAGTGAAGCAATTACAAGATAAGAAGGAAGAGTTAATGAAGGTATCTAATTTGAGTACAAATGCCACTACTCAAGATGGCTCTAGTAGTTCAAATATACAACCCTTTGTTATTGTTCAACCTTTTCCTGGAGGACTTGAGATTGTGTGCAGTTACAGCTTCAACAAGTGTTTGTTTCCTTTATCAAGAGAACCGATAATGACTGGAATTGATTATTCTGAACTGCAAAGAAAGCTCACCGAAGCAATATCATGCTCAAGTTTGCAAGAGAGTTCATTTGAACCTGAAATCGACAATATCTTGAGATAA
Protein sequence

>Msa0892380

MEENKKWMHKETERQRRQEMTNLCTNLRSLLPIEYIKGKRSISDHVNEATNYINHLQNKVKQLQDKKEELMKVSNLSTNATTQDGSSSSNIQPFVIVQPFPGGLEIVCSYSFNKCLFPLSREPIMTGIDYSELQRKLTEAISCSSLQESSFEPEIDNILR*