Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0925200 | AET02642.1 | 79.245 | 53 | 11 | 0 | 25 | 77 | 22 | 74 | 6.92e-20 | 90.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0319860 | Msa0925200 | 0.807247 | 5.549088e-50 | -8.615850e-47 |
| Msa0890270 | Msa0925200 | 0.822211 | 2.674270e-53 | -8.615850e-47 |
| Msa0925200 | Msa0925210 | 0.851634 | 7.810634e-61 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0925200 | MtrunA17_Chr8g0355431 | 79.245 | 53 | 11 | 0 | 25 | 77 | 22 | 74 | 6.36e-24 | 90.5 |
| Msa0925200 | MtrunA17_Chr5g0430521 | 62.338 | 77 | 12 | 2 | 1 | 77 | 66 | 125 | 9.88e-23 | 84.3 |
| Msa0925200 | MtrunA17_Chr8g0355501 | 78.846 | 52 | 11 | 0 | 26 | 77 | 23 | 74 | 1.76e-22 | 89.0 |
| Msa0925200 | MtrunA17_Chr8g0358571 | 48.182 | 110 | 19 | 2 | 2 | 77 | 1 | 106 | 6.66e-22 | 84.0 |
| Msa0925200 | MtrunA17_Chr4g0074241 | 62.338 | 77 | 12 | 2 | 1 | 77 | 66 | 125 | 9.75e-22 | 84.3 |
| Msa0925200 | MtrunA17_Chr2g0332321 | 63.636 | 77 | 11 | 2 | 1 | 77 | 108 | 167 | 1.04e-21 | 87.0 |
| Msa0925200 | MtrunA17_Chr6g0467971 | 54.688 | 64 | 23 | 1 | 20 | 77 | 3 | 66 | 1.38e-16 | 71.2 |
| Msa0925200 | MtrunA17_Chr3g0087111 | 50.704 | 71 | 29 | 1 | 13 | 77 | 87 | 157 | 3.02e-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGCTTATGAACTCAGTAAA+TGG | 0.324117 | 6_2:+68006278 | Msa0925200:CDS |
| GAGGAGCAAAGGAATGTAAC+TGG | 0.474329 | 6_2:+68006080 | Msa0925200:CDS |
| AGATTCTGATGAAGGAAGTT+CGG | 0.488984 | 6_2:+68006199 | Msa0925200:intron |
| AATGTAACTGGTTCATCATC+CGG | 0.497954 | 6_2:+68006092 | Msa0925200:CDS |
| GATGCACAAGATTCTGATGA+AGG | 0.522769 | 6_2:+68006191 | Msa0925200:intron |
| TTCATAAGCTCATATACCTT+AGG | 0.524558 | 6_2:-68006266 | None:intergenic |
| AACAATTGGAGGAGGAGCAA+AGG | 0.543896 | 6_2:+68006069 | None:intergenic |
| AAATGGCAAAAGAAGAAAAG+AGG | 0.547114 | 6_2:+68006295 | Msa0925200:CDS |
| ACATAATGAAGAACAATTGG+AGG | 0.553065 | 6_2:+68006058 | None:intergenic |
| TAATGAAGAACAATTGGAGG+AGG | 0.591143 | 6_2:+68006061 | None:intergenic |
| GCACAAGATGAGTATACCTA+AGG | 0.607751 | 6_2:+68006250 | Msa0925200:CDS |
| GTAACTGGTTCATCATCCGG+TGG | 0.691755 | 6_2:+68006095 | Msa0925200:CDS |
| ATTGACGTATTGAATGACAG+TGG | 0.710417 | 6_2:+68006323 | Msa0925200:CDS |
| AAAATGCAAAGCAAAGACTG+AGG | 0.715372 | 6_2:+68006228 | Msa0925200:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAAGGAGCAAAAAGAAG+AGG | + | chr6_2:68006124-68006143 | Msa0925200:CDS | 30.0% | |
| TTCATAAGCTCATATACCTT+AGG | - | chr6_2:68006269-68006288 | None:intergenic | 30.0% | |
| AAATGGCAAAAGAAGAAAAG+AGG | + | chr6_2:68006295-68006314 | Msa0925200:CDS | 30.0% | |
| ! | AATGTAACTGGTTCATCATC+CGG | + | chr6_2:68006092-68006111 | Msa0925200:CDS | 35.0% |
| ATCCGGTGGTGATAAAAATA+AGG | + | chr6_2:68006109-68006128 | Msa0925200:CDS | 35.0% | |
| !!! | CTCCTTATTTTTATCACCAC+CGG | - | chr6_2:68006114-68006133 | None:intergenic | 35.0% |
| GGCTAAAGGTAAAGAAAAGA+AGG | + | chr6_2:68006145-68006164 | Msa0925200:intron | 35.0% | |
| AGATTCTGATGAAGGAAGTT+CGG | + | chr6_2:68006199-68006218 | Msa0925200:intron | 35.0% | |
| AAAATGCAAAGCAAAGACTG+AGG | + | chr6_2:68006228-68006247 | Msa0925200:CDS | 35.0% | |
| GAGCTTATGAACTCAGTAAA+TGG | + | chr6_2:68006278-68006297 | Msa0925200:CDS | 35.0% | |
| ATTGACGTATTGAATGACAG+TGG | + | chr6_2:68006323-68006342 | Msa0925200:CDS | 35.0% | |
| GAGCAAAAAGAAGAGGCTAA+AGG | + | chr6_2:68006131-68006150 | Msa0925200:CDS | 40.0% | |
| GATGCACAAGATTCTGATGA+AGG | + | chr6_2:68006191-68006210 | Msa0925200:intron | 40.0% | |
| GCACAAGATGAGTATACCTA+AGG | + | chr6_2:68006250-68006269 | Msa0925200:CDS | 40.0% | |
| GAGGAGCAAAGGAATGTAAC+TGG | + | chr6_2:68006080-68006099 | Msa0925200:CDS | 45.0% | |
| ! | GTAACTGGTTCATCATCCGG+TGG | + | chr6_2:68006095-68006114 | Msa0925200:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 68006071 | 68006349 | 68006071 | ID=Msa0925200;Name=Msa0925200 |
| chr6_2 | mRNA | 68006071 | 68006349 | 68006071 | ID=Msa0925200-mRNA-1;Parent=Msa0925200;Name=Msa0925200-mRNA-1;_AED=0.45;_eAED=0.59;_QI=0|0|0|1|0|0.5|2|0|77 |
| chr6_2 | exon | 68006071 | 68006152 | 68006071 | ID=Msa0925200-mRNA-1:exon:10784;Parent=Msa0925200-mRNA-1 |
| chr6_2 | exon | 68006201 | 68006349 | 68006201 | ID=Msa0925200-mRNA-1:exon:10785;Parent=Msa0925200-mRNA-1 |
| chr6_2 | CDS | 68006071 | 68006152 | 68006071 | ID=Msa0925200-mRNA-1:cds;Parent=Msa0925200-mRNA-1 |
| chr6_2 | CDS | 68006201 | 68006349 | 68006201 | ID=Msa0925200-mRNA-1:cds;Parent=Msa0925200-mRNA-1 |
| Gene Sequence |
| Protein sequence |
