AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0890270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 93 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTTGAATTCTAGTTTA+TGG 0.087440 6_1:+63105342 None:intergenic
TAATGTTCAGTGAATTTAAT+TGG 0.170281 6_1:+63101335 None:intergenic
GATAAAATCAGAGTAAATTT+TGG 0.187182 6_1:+63101102 None:intergenic
ACTACCTACGGGTACAATAT+TGG 0.250194 6_1:+63105270 None:intergenic
CTACCTACGGGTACAATATT+GGG 0.270957 6_1:+63105271 None:intergenic
CATCATAGTGATTTAATAAT+AGG 0.271187 6_1:+63105473 None:intergenic
TGATTTAATAATAGGCATTA+AGG 0.279628 6_1:+63105481 None:intergenic
GATGATAATGGGAATGAGTA+TGG 0.285381 6_1:+63105190 None:intergenic
ATATCTTCAGACACAATTCT+TGG 0.288086 6_1:+63101429 None:intergenic
TTGAGGAATCAAGATGATAA+TGG 0.299145 6_1:+63105178 None:intergenic
CGTACTAAACATGCCTTATA+TGG 0.307191 6_1:-63101291 Msa0890270:three_prime_UTR
ATTTCCTTATAAACGACATT+AGG 0.311271 6_1:+63101128 None:intergenic
AACACCTAATGTCGTTTATA+AGG 0.314579 6_1:-63101132 Msa0890270:three_prime_UTR
GACTTCAACTAGCAAGAGTT+TGG 0.314742 6_1:-63101528 Msa0890270:CDS
AATAGGAATGAAAAGATAAA+TGG 0.317030 6_1:+63105318 None:intergenic
CATCCACTCTACTTGTTAGT+TGG 0.339684 6_1:-63105142 Msa0890270:five_prime_UTR
ACAAACGTACATACCATATA+AGG 0.341381 6_1:+63101278 None:intergenic
TTTATAAGCAATCATGCATT+TGG 0.341879 6_1:-63105508 Msa0890270:five_prime_UTR
TGCTTATAAAATAAATGTAT+TGG 0.355301 6_1:+63105521 None:intergenic
ACCAACAATGTCTGCAAACT+TGG 0.361386 6_1:+63102641 None:intergenic
TGAGGAATCAAGATGATAAT+GGG 0.368880 6_1:+63105179 None:intergenic
TCAACCCTCATGGTGAGCTT+TGG 0.373381 6_1:+63101195 None:intergenic
TCTGCAAACTTGGTGAGCTT+TGG 0.374688 6_1:+63102651 None:intergenic
TAAATGTAAGAATATTTAGA+TGG 0.382741 6_1:+63105226 None:intergenic
ATAAATGTATTGGTTCAATT+AGG 0.400332 6_1:+63105531 None:intergenic
AAAAGAATGAGCTAAACTTT+AGG 0.412116 6_1:-63105743 None:intergenic
GTTGGTGCAAAATCAACCTT+AGG 0.422132 6_1:-63105089 Msa0890270:five_prime_UTR
GATAATACTACCTCACATAA+TGG 0.424979 6_1:+63105375 None:intergenic
CACAATTCTTGGCTTCTTTG+TGG 0.426011 6_1:+63101440 None:intergenic
TGTTGAGCATGTAGATTCAT+TGG 0.431873 6_1:-63101495 Msa0890270:CDS
GTATTGGTTCAATTAGGTCT+TGG 0.436060 6_1:+63105537 None:intergenic
ACTGCACGAGCAAGTCTTTG+AGG 0.442425 6_1:+63105110 None:intergenic
TAAACAGTTCTTTCTAATGC+AGG 0.449641 6_1:-63102681 Msa0890270:intron
TAATCATGTAGAACAAGTTA+TGG 0.449981 6_1:+63105691 None:intergenic
CAGGATGTTAGCGAAGGTAC+TGG 0.455813 6_1:-63101585 Msa0890270:exon
ACTTCAACTAGCAAGAGTTT+GGG 0.458881 6_1:-63101527 Msa0890270:CDS
CTATTATTAAATCACTATGA+TGG 0.460354 6_1:-63105472 Msa0890270:five_prime_UTR
TATTATTAAATCACTATGAT+GGG 0.468014 6_1:-63105471 Msa0890270:five_prime_UTR
ACCATGAGGGTTGAAGGTAT+TGG 0.468858 6_1:-63101186 Msa0890270:three_prime_UTR
TGGCACGTAAGAGTAGGAAA+TGG 0.474877 6_1:+63105653 None:intergenic
TTTGAATAATAGAGATATAG+TGG 0.478842 6_1:+63105633 None:intergenic
AAGCTCACCATGAGGGTTGA+AGG 0.480890 6_1:-63101192 Msa0890270:three_prime_UTR
TTCATTGGATGCATTGTTGT+GGG 0.481078 6_1:-63101480 Msa0890270:CDS
TTGGTCTACTTTGATATGAG+TGG 0.492773 6_1:+63105556 None:intergenic
ACCAAGTTTGCAGACATTGT+TGG 0.493260 6_1:-63102642 Msa0890270:five_prime_UTR
GGCACGTAAGAGTAGGAAAT+GGG 0.519264 6_1:+63105654 None:intergenic
CTTATCGTGTATTAAAATTG+TGG 0.520182 6_1:-63105432 Msa0890270:five_prime_UTR
TTGCAGACATTGTTGGTGGA+AGG 0.522383 6_1:-63102635 Msa0890270:five_prime_UTR
ATGATAATGGGAATGAGTAT+GGG 0.523802 6_1:+63105191 None:intergenic
CAAAGACTTGCTCGTGCAGT+TGG 0.525796 6_1:-63105107 Msa0890270:five_prime_UTR
CATATAAGCATACCACTGTC+TGG 0.528804 6_1:+63101683 None:intergenic
GCCTCGGAATGATGAGGAAG+AGG 0.530416 6_1:-63101743 Msa0890270:five_prime_UTR
AAGAGTAGGAAATGGGAGGA+AGG 0.531532 6_1:+63105661 None:intergenic
ATTCATTGGATGCATTGTTG+TGG 0.534054 6_1:-63101481 Msa0890270:CDS
GGATGCATTGTTGTGGGTGA+AGG 0.539236 6_1:-63101474 Msa0890270:CDS
TGTTGGTGGAAGGAGACATT+GGG 0.548332 6_1:-63102625 Msa0890270:intron
ATGCGTTAGCAAAATTCGTA+CGG 0.549711 6_1:+63101226 None:intergenic
TTCGACACAAGGCGTCCCTA+AGG 0.550052 6_1:+63105073 None:intergenic
AAGATCTATGCTGTCAGTAA+TGG 0.555254 6_1:-63101390 Msa0890270:CDS
TTAGGGACGCCTTGTGTCGA+AGG 0.555407 6_1:-63105071 Msa0890270:five_prime_UTR
AAGTTTGCAGACATTGTTGG+TGG 0.561560 6_1:-63102639 Msa0890270:five_prime_UTR
GCCTCTTCCTCATCATTCCG+AGG 0.565375 6_1:+63101742 None:intergenic
TTGTTGGTGGAAGGAGACAT+TGG 0.567027 6_1:-63102626 Msa0890270:five_prime_UTR
CAACTAACAAGTAGAGTGGA+TGG 0.571302 6_1:+63105143 None:intergenic
AGCAAGAGTTTGGGAAAGAG+AGG 0.574732 6_1:-63101518 Msa0890270:CDS
ACCAATACCTTCAACCCTCA+TGG 0.576505 6_1:+63101185 None:intergenic
GTCCATAAACTAGAATTCAA+AGG 0.583231 6_1:-63105344 Msa0890270:five_prime_UTR
AGGATGTTAGCGAAGGTACT+GGG 0.583687 6_1:-63101584 Msa0890270:CDS
ATATAGTGGCACGTAAGAGT+AGG 0.588048 6_1:+63105647 None:intergenic
AAGGATGAAAACATGGCTGC+TGG 0.591758 6_1:-63101552 Msa0890270:CDS
CGTGTATTAAAATTGTGGCA+AGG 0.594806 6_1:-63105427 Msa0890270:five_prime_UTR
GTATTGGTTCTCGATGATGA+AGG 0.595746 6_1:-63101170 Msa0890270:three_prime_UTR
TATTGGTTCTCGATGATGAA+GGG 0.602986 6_1:-63101169 Msa0890270:three_prime_UTR
TACCTACGGGTACAATATTG+GGG 0.607294 6_1:+63105272 None:intergenic
TTCTCTCTGTCCATTATGTG+AGG 0.613703 6_1:-63105385 Msa0890270:five_prime_UTR
TGATAATGGGAATGAGTATG+GGG 0.616867 6_1:+63105192 None:intergenic
AATATTTAGATGGCTATGTG+AGG 0.621492 6_1:+63105236 None:intergenic
TTTAATCAGGATGTTAGCGA+AGG 0.622432 6_1:-63101591 Msa0890270:intron
TTTAATACACGATAAGAGAG+TGG 0.624634 6_1:+63105439 None:intergenic
GTCTCAGCCTCGGAATGATG+AGG 0.625440 6_1:-63101749 Msa0890270:intron
AGCTCCAAAGCTCACCATGA+GGG 0.629655 6_1:-63101199 Msa0890270:three_prime_UTR
AGGTACTGGGTCAAACAACA+AGG 0.645935 6_1:-63101571 Msa0890270:CDS
AGACCAACTAACAAGTAGAG+TGG 0.651051 6_1:+63105139 None:intergenic
AAGGTACATTCTCAATCCCA+CGG 0.653671 6_1:-63105408 Msa0890270:five_prime_UTR
ATTATTAAATCACTATGATG+GGG 0.655778 6_1:-63105470 Msa0890270:five_prime_UTR
TTGGTGCAAAATCAACCTTA+GGG 0.656431 6_1:-63105088 Msa0890270:five_prime_UTR
AAACAACAAGGATGAAAACA+TGG 0.656642 6_1:-63101559 Msa0890270:CDS
TAATGGACAGAGAGAACCGT+GGG 0.663616 6_1:+63105392 None:intergenic
AAGCTCCAAAGCTCACCATG+AGG 0.672428 6_1:-63101200 Msa0890270:three_prime_UTR
ACGTAAGAGTAGGAAATGGG+AGG 0.685588 6_1:+63105657 None:intergenic
TACCCCAATATTGTACCCGT+AGG 0.701992 6_1:-63105274 Msa0890270:five_prime_UTR
ACTAGCTGATAACCAGACAG+TGG 0.715970 6_1:-63101695 Msa0890270:intron
ATAATGGACAGAGAGAACCG+TGG 0.803544 6_1:+63105391 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTTAATATAAATAAAAAGA+TGG - chr6_1:63101895-63101914 Msa0890270:intron 10.0%
!! TCTAATTATTTATTTGTATT+TGG - chr6_1:63102350-63102369 Msa0890270:intron 10.0%
!!! TTATAAAATTATTTTCTAAC+TGG - chr6_1:63103646-63103665 Msa0890270:intron 10.0%
!!! TATAAAATTATTTTCTAACT+GGG - chr6_1:63103647-63103666 Msa0890270:intron 10.0%
!! ATTATAAATCTTTCATAATT+TGG + chr6_1:63104750-63104769 None:intergenic 10.0%
!! ATAAAAATCATAATTATTAG+AGG + chr6_1:63104904-63104923 None:intergenic 10.0%
!! ATTAAATGTATATTTCATAA+GGG + chr6_1:63105237-63105256 None:intergenic 10.0%
!! TGCTTATAAAATAAATGTAT+TGG + chr6_1:63101324-63101343 None:intergenic 15.0%
!! TATTATTAAATCACTATGAT+GGG - chr6_1:63101371-63101390 Msa0890270:CDS 15.0%
!! TAAATGTAAGAATATTTAGA+TGG + chr6_1:63101619-63101638 None:intergenic 15.0%
!! ATTATAATATTATGCCAATT+GGG - chr6_1:63102267-63102286 Msa0890270:intron 15.0%
!! ATATAAATAATGTATGGTAT+GGG + chr6_1:63103181-63103200 None:intergenic 15.0%
!!! TTTGAGATATAAATAATGTA+TGG + chr6_1:63103187-63103206 None:intergenic 15.0%
!! GAATTATTTAATCTAATTCA+TGG + chr6_1:63103232-63103251 None:intergenic 15.0%
!! AATATAAATGTTAACATAAG+AGG + chr6_1:63103580-63103599 None:intergenic 15.0%
!!! TATACTTTTCAAATCAAATA+TGG + chr6_1:63103894-63103913 None:intergenic 15.0%
!! CTTCAAAAAAAAAAAAAACA+TGG + chr6_1:63104261-63104280 None:intergenic 15.0%
!!! CATGTTTTTTTTTTTTTTGA+AGG - chr6_1:63104259-63104278 Msa0890270:intron 15.0%
!! GATTAAATGTATATTTCATA+AGG + chr6_1:63105238-63105257 None:intergenic 15.0%
!! TATGAAATATACATTTAATC+AGG - chr6_1:63105238-63105257 Msa0890270:five_prime_UTR 15.0%
!! TTTGAATAATAGAGATATAG+TGG + chr6_1:63101212-63101231 None:intergenic 20.0%
!! GTGGATGAATAAAATAAAAA+TGG + chr6_1:63101270-63101289 None:intergenic 20.0%
!!! ATAAATGTATTGGTTCAATT+AGG + chr6_1:63101314-63101333 None:intergenic 20.0%
!! TGATTTAATAATAGGCATTA+AGG + chr6_1:63101364-63101383 None:intergenic 20.0%
!! CATCATAGTGATTTAATAAT+AGG + chr6_1:63101372-63101391 None:intergenic 20.0%
!! CTATTATTAAATCACTATGA+TGG - chr6_1:63101370-63101389 Msa0890270:CDS 20.0%
!! ATTATTAAATCACTATGATG+GGG - chr6_1:63101372-63101391 Msa0890270:CDS 20.0%
!! AATAGGAATGAAAAGATAAA+TGG + chr6_1:63101527-63101546 None:intergenic 20.0%
!!! CTACAAGAGTTTTAAATAAT+AGG + chr6_1:63101544-63101563 None:intergenic 20.0%
!! GATTATAATATTATGCCAAT+TGG - chr6_1:63102266-63102285 Msa0890270:intron 20.0%
!! TTATAATATTATGCCAATTG+GGG - chr6_1:63102268-63102287 Msa0890270:intron 20.0%
!!! TCTATATGAATGTATTTTTG+TGG - chr6_1:63102428-63102447 Msa0890270:intron 20.0%
!! TCATACTATATATAAATCCA+AGG - chr6_1:63102652-63102671 Msa0890270:five_prime_UTR 20.0%
!!! GGTATTTGTTAACAAAATTA+GGG + chr6_1:63102697-63102716 None:intergenic 20.0%
!! GATAAAATAATAACAAATGC+AGG - chr6_1:63102855-63102874 Msa0890270:intron 20.0%
!! TAGCACCATTAATTTATATA+AGG - chr6_1:63102921-63102940 Msa0890270:intron 20.0%
!! GATATAAATAATGTATGGTA+TGG + chr6_1:63103182-63103201 None:intergenic 20.0%
!! ATTTAATCTAATTCATGGAT+GGG + chr6_1:63103227-63103246 None:intergenic 20.0%
!! TATTTAATCTAATTCATGGA+TGG + chr6_1:63103228-63103247 None:intergenic 20.0%
!!! GAAATTTTGAACAATTTGAA+AGG + chr6_1:63104057-63104076 None:intergenic 20.0%
!!! AGAAGAGAAAAAAAGTATAA+GGG + chr6_1:63104124-63104143 None:intergenic 20.0%
!!! TAGAAGAGAAAAAAAGTATA+AGG + chr6_1:63104125-63104144 None:intergenic 20.0%
!!! AGACTTCATTTTTATTCATT+TGG - chr6_1:63104366-63104385 Msa0890270:intron 20.0%
!! TGTTGCATAAAGATATAAAA+TGG - chr6_1:63104650-63104669 Msa0890270:intron 20.0%
!! TAATGTTCAGTGAATTTAAT+TGG + chr6_1:63105510-63105529 None:intergenic 20.0%
! TAATCATGTAGAACAAGTTA+TGG + chr6_1:63101154-63101173 None:intergenic 25.0%
! TTTATAAGCAATCATGCATT+TGG - chr6_1:63101334-63101353 Msa0890270:CDS 25.0%
! CTTATCGTGTATTAAAATTG+TGG - chr6_1:63101410-63101429 Msa0890270:CDS 25.0%
!!! TTCCTTTGAATTCTAGTTTA+TGG + chr6_1:63101503-63101522 None:intergenic 25.0%
! TTTATGACTACATGATTGTA+CGG + chr6_1:63101825-63101844 None:intergenic 25.0%
! TAGACATAAAAACATAGATG+AGG + chr6_1:63102412-63102431 None:intergenic 25.0%
!!! TGAATGTATTTTTGTGGTTT+AGG - chr6_1:63102434-63102453 Msa0890270:intron 25.0%
!! GGGTATTTGTTAACAAAATT+AGG + chr6_1:63102698-63102717 None:intergenic 25.0%
!!! TGTTGTTTTGACATCTAATA+GGG + chr6_1:63102719-63102738 None:intergenic 25.0%
!!! TTGTTGTTTTGACATCTAAT+AGG + chr6_1:63102720-63102739 None:intergenic 25.0%
! TGCATCTGTTATTATTTGTT+TGG + chr6_1:63102763-63102782 None:intergenic 25.0%
! AAACAAATAATAACAGATGC+AGG - chr6_1:63102762-63102781 Msa0890270:intron 25.0%
! GATTAAATAATAGCAGATTG+TGG - chr6_1:63102802-63102821 Msa0890270:intron 25.0%
! CCATGCCTTATATAAATTAA+TGG + chr6_1:63102929-63102948 None:intergenic 25.0%
! CCATTAATTTATATAAGGCA+TGG - chr6_1:63102926-63102945 Msa0890270:intron 25.0%
! TGAAATGCTAAAGTCTAAAA+AGG + chr6_1:63103028-63103047 None:intergenic 25.0%
! ATAATGCATATAAGAACCTT+TGG + chr6_1:63103135-63103154 None:intergenic 25.0%
! TATGACAACAGATATGAAAT+TGG + chr6_1:63103550-63103569 None:intergenic 25.0%
!! ATTTTGAATAGTTTCAACGT+TGG - chr6_1:63103619-63103638 Msa0890270:intron 25.0%
! GTGTAATTGTCATATAAGAT+AGG + chr6_1:63103694-63103713 None:intergenic 25.0%
! ATACAACAAACAAAATCACA+GGG + chr6_1:63103755-63103774 None:intergenic 25.0%
! ATGAAGAATTTGTGATCTTA+AGG - chr6_1:63103859-63103878 Msa0890270:intron 25.0%
! TTCAAAATTTCTGCTAACAA+TGG - chr6_1:63104066-63104085 Msa0890270:intron 25.0%
!!! TTTGGTTCTCTAATTTTCTT+TGG - chr6_1:63104384-63104403 Msa0890270:intron 25.0%
! AATCTCATTATAGAATTGAC+AGG + chr6_1:63104463-63104482 None:intergenic 25.0%
!!! AAAAGTTACTTTTAGCACAT+GGG - chr6_1:63104537-63104556 Msa0890270:intron 25.0%
!! TATGAAAAGTTTCTTTTGAC+AGG + chr6_1:63104981-63105000 None:intergenic 25.0%
! ATTTCCTTATAAACGACATT+AGG + chr6_1:63105717-63105736 None:intergenic 25.0%
TTTAATACACGATAAGAGAG+TGG + chr6_1:63101406-63101425 None:intergenic 30.0%
GATAATACTACCTCACATAA+TGG + chr6_1:63101470-63101489 None:intergenic 30.0%
GTCCATAAACTAGAATTCAA+AGG - chr6_1:63101498-63101517 Msa0890270:CDS 30.0%
AATATTTAGATGGCTATGTG+AGG + chr6_1:63101609-63101628 None:intergenic 30.0%
!! ATGATAATGGGAATGAGTAT+GGG + chr6_1:63101654-63101673 None:intergenic 30.0%
TGAGGAATCAAGATGATAAT+GGG + chr6_1:63101666-63101685 None:intergenic 30.0%
TTGAGGAATCAAGATGATAA+TGG + chr6_1:63101667-63101686 None:intergenic 30.0%
TCATGTAGTCATAAACTCAT+AGG - chr6_1:63101830-63101849 Msa0890270:intron 30.0%
TAAAGTTTCCTCCTTTCAAT+AGG + chr6_1:63101880-63101899 None:intergenic 30.0%
TTATTGCATCTCATGTTTGA+TGG - chr6_1:63101928-63101947 Msa0890270:intron 30.0%
AAAAGATTATACGTCATGCA+TGG - chr6_1:63102080-63102099 Msa0890270:intron 30.0%
! TTTTGTCTTTAGGTGTCAAA+TGG - chr6_1:63102240-63102259 Msa0890270:intron 30.0%
TTTGTCTTTAGGTGTCAAAT+GGG - chr6_1:63102241-63102260 Msa0890270:intron 30.0%
!!! TTTGGCTTTTGACTTTTATG+TGG - chr6_1:63102368-63102387 Msa0890270:intron 30.0%
! ACCTAAAATGTTGGTTGTAA+GGG + chr6_1:63102469-63102488 None:intergenic 30.0%
! TACCTAAAATGTTGGTTGTA+AGG + chr6_1:63102470-63102489 None:intergenic 30.0%
CTCATCAATACCTAAAATGT+TGG + chr6_1:63102478-63102497 None:intergenic 30.0%
!!! GTTGTTTTGACATCTAATAG+GGG + chr6_1:63102718-63102737 None:intergenic 30.0%
AATAATAACAAATGCAGGCA+TGG - chr6_1:63102860-63102879 Msa0890270:intron 30.0%
!! ATTAATGGTGCTATGCAAAT+GGG + chr6_1:63102914-63102933 None:intergenic 30.0%
!! AATTAATGGTGCTATGCAAA+TGG + chr6_1:63102915-63102934 None:intergenic 30.0%
ATTAATGGAGCTATGCAAAT+GGG + chr6_1:63102977-63102996 None:intergenic 30.0%
AATTAATGGAGCTATGCAAA+TGG + chr6_1:63102978-63102997 None:intergenic 30.0%
! ATTACTTGATGATAGTGACA+CGG + chr6_1:63103070-63103089 None:intergenic 30.0%
AAATAATGTATGGTATGGGA+AGG + chr6_1:63103177-63103196 None:intergenic 30.0%
GGAATTAAGAAACAAACCTT+TGG + chr6_1:63103529-63103548 None:intergenic 30.0%
GATACAACAAACAAAATCAC+AGG + chr6_1:63103756-63103775 None:intergenic 30.0%
GTATAAGGGACAATTTCTAT+TGG + chr6_1:63104110-63104129 None:intergenic 30.0%
TAAACAGTTCTTTCTAATGC+AGG - chr6_1:63104161-63104180 Msa0890270:intron 30.0%
TATCATATAAAGGGACAACA+TGG + chr6_1:63104295-63104314 None:intergenic 30.0%
GTGCTTCTGTATCATATAAA+GGG + chr6_1:63104304-63104323 None:intergenic 30.0%
TGTGCTTCTGTATCATATAA+AGG + chr6_1:63104305-63104324 None:intergenic 30.0%
!!! GAAAAGTTACTTTTAGCACA+TGG - chr6_1:63104536-63104555 Msa0890270:intron 30.0%
!!! AAAGTTACTTTTAGCACATG+GGG - chr6_1:63104538-63104557 Msa0890270:intron 30.0%
! TGATTTTTATACCAAAGCTG+TGG - chr6_1:63104914-63104933 Msa0890270:intron 30.0%
AAACACACTAAAACCAAAGT+CGG + chr6_1:63105188-63105207 None:intergenic 30.0%
AAACAACAAGGATGAAAACA+TGG - chr6_1:63105283-63105302 Msa0890270:five_prime_UTR 30.0%
ATATCTTCAGACACAATTCT+TGG + chr6_1:63105416-63105435 None:intergenic 30.0%
ACAAACGTACATACCATATA+AGG + chr6_1:63105567-63105586 None:intergenic 30.0%
! AACACCTAATGTCGTTTATA+AGG - chr6_1:63105710-63105729 Msa0890270:five_prime_UTR 30.0%
TTGGTCTACTTTGATATGAG+TGG + chr6_1:63101289-63101308 None:intergenic 35.0%
!! GTATTGGTTCAATTAGGTCT+TGG + chr6_1:63101308-63101327 None:intergenic 35.0%
CGTGTATTAAAATTGTGGCA+AGG - chr6_1:63101415-63101434 Msa0890270:CDS 35.0%
!! TTTGTTTTCTGACTACCTAC+GGG + chr6_1:63101586-63101605 None:intergenic 35.0%
!! GTTTGTTTTCTGACTACCTA+CGG + chr6_1:63101587-63101606 None:intergenic 35.0%
!! TGATAATGGGAATGAGTATG+GGG + chr6_1:63101653-63101672 None:intergenic 35.0%
!! GATGATAATGGGAATGAGTA+TGG + chr6_1:63101655-63101674 None:intergenic 35.0%
! GATGGAAGCTTAGAATTTTG+AGG + chr6_1:63101684-63101703 None:intergenic 35.0%
TTGGTGCAAAATCAACCTTA+GGG - chr6_1:63101754-63101773 Msa0890270:intron 35.0%
TAGTCATAAACTCATAGGCT+AGG - chr6_1:63101835-63101854 Msa0890270:intron 35.0%
TCCTCCTTTCAATAGGAAAA+GGG + chr6_1:63101873-63101892 None:intergenic 35.0%
TTCCTCCTTTCAATAGGAAA+AGG + chr6_1:63101874-63101893 None:intergenic 35.0%
! TGGAAATGTCTCATCTTTTG+TGG - chr6_1:63101948-63101967 Msa0890270:intron 35.0%
!!! GTTTGTCGTTTTGAGAACAA+AGG - chr6_1:63102003-63102022 Msa0890270:intron 35.0%
ATGGCATATAGTTGTGTCTT+AGG - chr6_1:63102104-63102123 Msa0890270:intron 35.0%
CGATTATCATTGTGCCAATT+GGG - chr6_1:63102139-63102158 Msa0890270:intron 35.0%
GATTATCATTGTGCCAATTG+GGG - chr6_1:63102140-63102159 Msa0890270:intron 35.0%
TGATTATCACTGTGCCAATT+GGG - chr6_1:63102163-63102182 Msa0890270:intron 35.0%
GATTTATCAGTGTGCCAATT+GGG - chr6_1:63102188-63102207 Msa0890270:intron 35.0%
ATTTATCAGTGTGCCAATTG+GGG - chr6_1:63102189-63102208 Msa0890270:intron 35.0%
GATTTGTCATTCTGCCAAAT+GGG - chr6_1:63102213-63102232 Msa0890270:intron 35.0%
ATTTGTCATTCTGCCAAATG+GGG - chr6_1:63102214-63102233 Msa0890270:intron 35.0%
TTGTCTTTAGGTGTCAAATG+GGG - chr6_1:63102242-63102261 Msa0890270:intron 35.0%
! CCTAAAATGTTGGTTGTAAG+GGG + chr6_1:63102468-63102487 None:intergenic 35.0%
AATAATAACAGATGCAGGCA+TGG - chr6_1:63102767-63102786 Msa0890270:intron 35.0%
CAACTAAAACTTCTGCAGTT+TGG + chr6_1:63102828-63102847 None:intergenic 35.0%
!! TTAATGGTGCTATGCAAATG+GGG + chr6_1:63102913-63102932 None:intergenic 35.0%
TTAATGGAGCTATGCAAATG+GGG + chr6_1:63102976-63102995 None:intergenic 35.0%
GCTATCATTATTGCATCCAA+AGG - chr6_1:63103116-63103135 Msa0890270:intron 35.0%
TTCACTGTCTACCAAATTCT+TGG + chr6_1:63103345-63103364 None:intergenic 35.0%
GCTTTCCACACTATAAATAC+AGG - chr6_1:63103410-63103429 Msa0890270:intron 35.0%
! GTTTTGCTGAAAGAAATCCA+TGG - chr6_1:63103500-63103519 Msa0890270:intron 35.0%
TGTTAAGCATGAATGAAGCA+TGG - chr6_1:63104611-63104630 Msa0890270:intron 35.0%
AAAAAGAGACAAAGACAGCA+AGG + chr6_1:63104698-63104717 None:intergenic 35.0%
ATCATTTCAAACCACAGCTT+TGG + chr6_1:63104928-63104947 None:intergenic 35.0%
TGCTTATATGTTTCCGACTT+TGG - chr6_1:63105172-63105191 Msa0890270:five_prime_UTR 35.0%
TTTAATCAGGATGTTAGCGA+AGG - chr6_1:63105251-63105270 Msa0890270:five_prime_UTR 35.0%
ACTTCAACTAGCAAGAGTTT+GGG - chr6_1:63105315-63105334 Msa0890270:five_prime_UTR 35.0%
TGTTGAGCATGTAGATTCAT+TGG - chr6_1:63105347-63105366 Msa0890270:five_prime_UTR 35.0%
ATTCATTGGATGCATTGTTG+TGG - chr6_1:63105361-63105380 Msa0890270:five_prime_UTR 35.0%
TTCATTGGATGCATTGTTGT+GGG - chr6_1:63105362-63105381 Msa0890270:five_prime_UTR 35.0%
AAGATCTATGCTGTCAGTAA+TGG - chr6_1:63105452-63105471 Msa0890270:five_prime_UTR 35.0%
CGTACTAAACATGCCTTATA+TGG - chr6_1:63105551-63105570 Msa0890270:five_prime_UTR 35.0%
ATGCGTTAGCAAAATTCGTA+CGG + chr6_1:63105619-63105638 None:intergenic 35.0%
!! TATTGGTTCTCGATGATGAA+GGG - chr6_1:63105673-63105692 Msa0890270:five_prime_UTR 35.0%
ATATAGTGGCACGTAAGAGT+AGG + chr6_1:63101198-63101217 None:intergenic 40.0%
AAGGTACATTCTCAATCCCA+CGG - chr6_1:63101434-63101453 Msa0890270:CDS 40.0%
TTCTCTCTGTCCATTATGTG+AGG - chr6_1:63101457-63101476 Msa0890270:CDS 40.0%
TACCTACGGGTACAATATTG+GGG + chr6_1:63101573-63101592 None:intergenic 40.0%
CTACCTACGGGTACAATATT+GGG + chr6_1:63101574-63101593 None:intergenic 40.0%
ACTACCTACGGGTACAATAT+TGG + chr6_1:63101575-63101594 None:intergenic 40.0%
CAACTAACAAGTAGAGTGGA+TGG + chr6_1:63101702-63101721 None:intergenic 40.0%
! CATCCACTCTACTTGTTAGT+TGG - chr6_1:63101700-63101719 Msa0890270:five_prime_UTR 40.0%
AGACCAACTAACAAGTAGAG+TGG + chr6_1:63101706-63101725 None:intergenic 40.0%
GTTGGTGCAAAATCAACCTT+AGG - chr6_1:63101753-63101772 Msa0890270:intron 40.0%
! AGTGTTTTTCCTTCGACACA+AGG + chr6_1:63101783-63101802 None:intergenic 40.0%
AGGAAAAACACTCAGAGGTA+AGG - chr6_1:63101791-63101810 Msa0890270:intron 40.0%
GGAAAAACACTCAGAGGTAA+GGG - chr6_1:63101792-63101811 Msa0890270:intron 40.0%
! AGAGCCCTTTTCCTATTGAA+AGG - chr6_1:63101866-63101885 Msa0890270:intron 40.0%
! TCATCTTTTGTGGCATTGCA+TGG - chr6_1:63101958-63101977 Msa0890270:intron 40.0%
ATTATACGTCATGCATGGCA+TGG - chr6_1:63102085-63102104 Msa0890270:intron 40.0%
TTGTGTCTTAGGTGCCAAAT+GGG - chr6_1:63102115-63102134 Msa0890270:intron 40.0%
ACAATGATAATCGCCCCATT+TGG + chr6_1:63102132-63102151 None:intergenic 40.0%
GCGATTATCATTGTGCCAAT+TGG - chr6_1:63102138-63102157 Msa0890270:intron 40.0%
ACAGTGATAATCACCCCAAT+TGG + chr6_1:63102156-63102175 None:intergenic 40.0%
GTGATTATCACTGTGCCAAT+TGG - chr6_1:63102162-63102181 Msa0890270:intron 40.0%
GATTATCACTGTGCCAATTG+GGG - chr6_1:63102164-63102183 Msa0890270:intron 40.0%
CGATTTATCAGTGTGCCAAT+TGG - chr6_1:63102187-63102206 Msa0890270:intron 40.0%
CGATTTGTCATTCTGCCAAA+TGG - chr6_1:63102212-63102231 Msa0890270:intron 40.0%
AAAGACAAAATCGCCCCATT+TGG + chr6_1:63102230-63102249 None:intergenic 40.0%
! AATGGGGCGATTTTGTCTTT+AGG - chr6_1:63102230-63102249 Msa0890270:intron 40.0%
! CCCCTTACAACCAACATTTT+AGG - chr6_1:63102465-63102484 Msa0890270:intron 40.0%
! AGTTTGTTTGCAGACTACCT+TGG + chr6_1:63102672-63102691 None:intergenic 40.0%
!! TTGTTTGGTGCAAAGCTGAA+TGG + chr6_1:63102748-63102767 None:intergenic 40.0%
AATGTATGGTATGGGAAGGA+AGG + chr6_1:63103173-63103192 None:intergenic 40.0%
ACTGTCTACCAAATTCTTGG+AGG + chr6_1:63103342-63103361 None:intergenic 40.0%
TGACCAAAAGCTAGAAATGG+TGG + chr6_1:63103436-63103455 None:intergenic 40.0%
GCATGACCAAAAGCTAGAAA+TGG + chr6_1:63103439-63103458 None:intergenic 40.0%
AAGAAATCCATGGCATCCAA+AGG - chr6_1:63103510-63103529 Msa0890270:intron 40.0%
AAACAAACCTTTGGATGCCA+TGG + chr6_1:63103520-63103539 None:intergenic 40.0%
ACCAAGTTTGCAGACATTGT+TGG - chr6_1:63104200-63104219 Msa0890270:intron 40.0%
ACCAACAATGTCTGCAAACT+TGG + chr6_1:63104204-63104223 None:intergenic 40.0%
AAGTTTGCAGACATTGTTGG+TGG - chr6_1:63104203-63104222 Msa0890270:intron 40.0%
CATGAATGAAGCATGGCAAT+TGG - chr6_1:63104618-63104637 Msa0890270:intron 40.0%
ATTTACCAATGTCTCAGCCT+CGG - chr6_1:63105083-63105102 Msa0890270:five_prime_UTR 40.0%
CATATAAGCATACCACTGTC+TGG + chr6_1:63105162-63105181 None:intergenic 40.0%
GACTTCAACTAGCAAGAGTT+TGG - chr6_1:63105314-63105333 Msa0890270:five_prime_UTR 40.0%
CACAATTCTTGGCTTCTTTG+TGG + chr6_1:63105405-63105424 None:intergenic 40.0%
!! GTATTGGTTCTCGATGATGA+AGG - chr6_1:63105672-63105691 Msa0890270:five_prime_UTR 40.0%
AAGAGTAGGAAATGGGAGGA+AGG + chr6_1:63101184-63101203 None:intergenic 45.0%
ACGTAAGAGTAGGAAATGGG+AGG + chr6_1:63101188-63101207 None:intergenic 45.0%
GGCACGTAAGAGTAGGAAAT+GGG + chr6_1:63101191-63101210 None:intergenic 45.0%
TGGCACGTAAGAGTAGGAAA+TGG + chr6_1:63101192-63101211 None:intergenic 45.0%
! TAATGGACAGAGAGAACCGT+GGG + chr6_1:63101453-63101472 None:intergenic 45.0%
!! ATAATGGACAGAGAGAACCG+TGG + chr6_1:63101454-63101473 None:intergenic 45.0%
TACCCCAATATTGTACCCGT+AGG - chr6_1:63101568-63101587 Msa0890270:CDS 45.0%
GTCGAAGGAAAAACACTCAG+AGG - chr6_1:63101786-63101805 Msa0890270:intron 45.0%
AAAACACTCAGAGGTAAGGG+CGG - chr6_1:63101795-63101814 Msa0890270:intron 45.0%
! GCCCTTTTCCTATTGAAAGG+AGG - chr6_1:63101869-63101888 Msa0890270:intron 45.0%
GTTGTGTCTTAGGTGCCAAA+TGG - chr6_1:63102114-63102133 Msa0890270:intron 45.0%
TGTGTCTTAGGTGCCAAATG+GGG - chr6_1:63102116-63102135 Msa0890270:intron 45.0%
CACTGATAAATCGCCCCAAT+TGG + chr6_1:63102180-63102199 None:intergenic 45.0%
GAATGACAAATCGCCCCAAT+TGG + chr6_1:63102205-63102224 None:intergenic 45.0%
AAGAAAAGTTTGCACACGCG+CGG - chr6_1:63102510-63102529 Msa0890270:intron 45.0%
CAATGCTGAGACTGAGAACA+TGG + chr6_1:63103260-63103279 None:intergenic 45.0%
CATGTTCTCAGTCTCAGCAT+TGG - chr6_1:63103258-63103277 Msa0890270:intron 45.0%
ATTCTTGGAGGTTGAGTCCA+GGG + chr6_1:63103330-63103349 None:intergenic 45.0%
AATTCTTGGAGGTTGAGTCC+AGG + chr6_1:63103331-63103350 None:intergenic 45.0%
GGACTCAACCTCCAAGAATT+TGG - chr6_1:63103331-63103350 Msa0890270:intron 45.0%
GGTGGCCTGTATTTATAGTG+TGG + chr6_1:63103418-63103437 None:intergenic 45.0%
!!! AGGCCACCATTTCTAGCTTT+TGG - chr6_1:63103430-63103449 Msa0890270:intron 45.0%
! TCTGCAAACTTGGTGAGCTT+TGG + chr6_1:63104194-63104213 None:intergenic 45.0%
! TTGCAGACATTGTTGGTGGA+AGG - chr6_1:63104207-63104226 Msa0890270:intron 45.0%
! TTGTTGGTGGAAGGAGACAT+TGG - chr6_1:63104216-63104235 Msa0890270:intron 45.0%
! TGTTGGTGGAAGGAGACATT+GGG - chr6_1:63104217-63104236 Msa0890270:intron 45.0%
GGAAGGAGACATTGGGTATA+AGG - chr6_1:63104224-63104243 Msa0890270:intron 45.0%
ACTAGCTGATAACCAGACAG+TGG - chr6_1:63105147-63105166 Msa0890270:five_prime_UTR 45.0%
!! AGGATGTTAGCGAAGGTACT+GGG - chr6_1:63105258-63105277 Msa0890270:five_prime_UTR 45.0%
!! AGGTACTGGGTCAAACAACA+AGG - chr6_1:63105271-63105290 Msa0890270:five_prime_UTR 45.0%
AAGGATGAAAACATGGCTGC+TGG - chr6_1:63105290-63105309 Msa0890270:five_prime_UTR 45.0%
AGCAAGAGTTTGGGAAAGAG+AGG - chr6_1:63105324-63105343 Msa0890270:five_prime_UTR 45.0%
! ACCATGAGGGTTGAAGGTAT+TGG - chr6_1:63105656-63105675 Msa0890270:five_prime_UTR 45.0%
ACCAATACCTTCAACCCTCA+TGG + chr6_1:63105660-63105679 None:intergenic 45.0%
!! ATAAGAAATTTATAAATTAA+TGG + chr6_1:63102992-63103011 None:intergenic 5.0%
!! ATTATTAAAAAAAAATATGA+TGG - chr6_1:63104721-63104740 Msa0890270:intron 5.0%
ACTGCACGAGCAAGTCTTTG+AGG + chr6_1:63101735-63101754 None:intergenic 50.0%
! CAAAGACTTGCTCGTGCAGT+TGG - chr6_1:63101735-63101754 Msa0890270:five_prime_UTR 50.0%
ATGCCAATTGGGGCGATATC+TGG - chr6_1:63102278-63102297 Msa0890270:intron 50.0%
GATCCAGATATCGCCCCAAT+TGG + chr6_1:63102284-63102303 None:intergenic 50.0%
TTGGGGCGATATCTGGATCA+TGG - chr6_1:63102285-63102304 Msa0890270:intron 50.0%
AGAAAAGTTTGCACACGCGC+GGG - chr6_1:63102511-63102530 Msa0890270:intron 50.0%
ACACGCGCGGGGAATAAATT+TGG - chr6_1:63102523-63102542 Msa0890270:intron 50.0%
CACGCGCGGGGAATAAATTT+GGG - chr6_1:63102524-63102543 Msa0890270:intron 50.0%
ACGCGCGGGGAATAAATTTG+GGG - chr6_1:63102525-63102544 Msa0890270:intron 50.0%
! AATGCTCTTTTGCCCATCCC+TGG - chr6_1:63103310-63103329 Msa0890270:intron 50.0%
! TGAGCATCTTTTGCGCATGC+AGG + chr6_1:63103370-63103389 None:intergenic 50.0%
GCACATGGGGTAGCAAGAAA+AGG - chr6_1:63104551-63104570 Msa0890270:intron 50.0%
TCATTCCGAGGCTGAGACAT+TGG + chr6_1:63105091-63105110 None:intergenic 50.0%
! CAGGATGTTAGCGAAGGTAC+TGG - chr6_1:63105257-63105276 Msa0890270:five_prime_UTR 50.0%
GGATGCATTGTTGTGGGTGA+AGG - chr6_1:63105368-63105387 Msa0890270:five_prime_UTR 50.0%
AAGCTCCAAAGCTCACCATG+AGG - chr6_1:63105642-63105661 Msa0890270:five_prime_UTR 50.0%
AGCTCCAAAGCTCACCATGA+GGG - chr6_1:63105643-63105662 Msa0890270:five_prime_UTR 50.0%
TCAACCCTCATGGTGAGCTT+TGG + chr6_1:63105650-63105669 None:intergenic 50.0%
AAGCTCACCATGAGGGTTGA+AGG - chr6_1:63105650-63105669 Msa0890270:five_prime_UTR 50.0%
! TTCGACACAAGGCGTCCCTA+AGG + chr6_1:63101772-63101791 None:intergenic 55.0%
TTAGGGACGCCTTGTGTCGA+AGG - chr6_1:63101771-63101790 Msa0890270:intron 55.0%
GAAAAGTTTGCACACGCGCG+GGG - chr6_1:63102512-63102531 Msa0890270:intron 55.0%
GGATGGGTCATGACAGAGAG+AGG + chr6_1:63103211-63103230 None:intergenic 55.0%
TGGAGGTTGAGTCCAGGGAT+GGG + chr6_1:63103325-63103344 None:intergenic 55.0%
TTGGAGGTTGAGTCCAGGGA+TGG + chr6_1:63103326-63103345 None:intergenic 55.0%
GTCTCAGCCTCGGAATGATG+AGG - chr6_1:63105093-63105112 Msa0890270:five_prime_UTR 55.0%
GCCTCGGAATGATGAGGAAG+AGG - chr6_1:63105099-63105118 Msa0890270:five_prime_UTR 55.0%
GCCTCTTCCTCATCATTCCG+AGG + chr6_1:63105103-63105122 None:intergenic 55.0%
CTACGTCTGCTCTCAGCAGC+AGG + chr6_1:63104779-63104798 None:intergenic 60.0%
GCTGCTGAGAGCAGACGTAG+TGG - chr6_1:63104779-63104798 Msa0890270:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 63101110 63105754 63101110 ID=Msa0890270;Name=Msa0890270
chr6_1 mRNA 63101110 63105754 63101110 ID=Msa0890270-mRNA-1;Parent=Msa0890270;Name=Msa0890270-mRNA-1;_AED=0.27;_eAED=0.31;_QI=839|0.33|0.75|1|0.66|0.5|4|224|89
chr6_1 exon 63101110 63101604 63101110 ID=Msa0890270-mRNA-1:exon:11516;Parent=Msa0890270-mRNA-1
chr6_1 exon 63101696 63101764 63101696 ID=Msa0890270-mRNA-1:exon:11515;Parent=Msa0890270-mRNA-1
chr6_1 exon 63102626 63102696 63102626 ID=Msa0890270-mRNA-1:exon:11514;Parent=Msa0890270-mRNA-1
chr6_1 exon 63105057 63105754 63105057 ID=Msa0890270-mRNA-1:exon:11513;Parent=Msa0890270-mRNA-1
chr6_1 five_prime_UTR 63105057 63105754 63105057 ID=Msa0890270-mRNA-1:five_prime_utr;Parent=Msa0890270-mRNA-1
chr6_1 five_prime_UTR 63102626 63102696 63102626 ID=Msa0890270-mRNA-1:five_prime_utr;Parent=Msa0890270-mRNA-1
chr6_1 five_prime_UTR 63101696 63101764 63101696 ID=Msa0890270-mRNA-1:five_prime_utr;Parent=Msa0890270-mRNA-1
chr6_1 five_prime_UTR 63101604 63101604 63101604 ID=Msa0890270-mRNA-1:five_prime_utr;Parent=Msa0890270-mRNA-1
chr6_1 CDS 63101334 63101603 63101334 ID=Msa0890270-mRNA-1:cds;Parent=Msa0890270-mRNA-1
chr6_1 three_prime_UTR 63101110 63101333 63101110 ID=Msa0890270-mRNA-1:three_prime_utr;Parent=Msa0890270-mRNA-1
Gene Sequence

>Msa0890270

ATGTTAGCGAAGGTACTGGGTCAAACAACAAGGATGAAAACATGGCTGCTGGTGACTTCAACTAGCAAGAGTTTGGGAAAGAGAGGTGTTGAGCATGTAGATTCATTGGATGCATTGTTGTGGGTGAAGGTGATGCATCTACCACAAAGAAGCCAAGAATTGTGTCTGAAGATATTGATTGACTTTCGAATAAGATCTATGCTGTCAGTAATGGTTGTTATTGTGTTTTCTGTTTTAAGTTTAATTCCAATTAAATTCACTGAACATTAA
Protein sequence

>Msa0890270

MLAKVLGQTTRMKTWLLVTSTSKSLGKRGVEHVDSLDALLWVKVMHLPQRSQELCLKILIDFRIRSMLSVMVVIVFSVLSLIPIKFTEH*