AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0319860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTGAAATTTGAAATTT+TGG 0.100484 2_4:-19948325 Msa0319860:three_prime_UTR
CTGTTGATTTAATTTATTTA+AGG 0.135415 2_4:+19948168 None:intergenic
CCCTCCGTACTTTGGAAAAT+TGG 0.175068 2_4:-19948222 Msa0319860:three_prime_UTR
TTTATGTTGCATTGAGGGTT+AGG 0.264134 2_4:+19948558 None:intergenic
GATTCATCTATTTCTGTTGT+TGG 0.310831 2_4:-19950105 Msa0319860:intron
AAATAAATCAATGTTACATT+TGG 0.339907 2_4:+19948250 None:intergenic
TTTCCTTTATGTTGCATTGA+GGG 0.366390 2_4:+19948553 None:intergenic
ATCTGATGCACTACGAATTC+CGG 0.369685 2_4:+19950125 None:intergenic
TGTTGATTTAATTTATTTAA+GGG 0.370038 2_4:+19948169 None:intergenic
TATGTTGGCCTGATTTCTTT+TGG 0.372033 2_4:-19949296 Msa0319860:intron
GGACGAGAGAGAATGGTCTA+TGG 0.391543 2_4:+19950304 None:intergenic
TTTATTCTCCCTCCGTACTT+TGG 0.391864 2_4:-19948230 Msa0319860:three_prime_UTR
TAAGTGTGATAACATAGAAA+TGG 0.418800 2_4:+19950173 None:intergenic
TTATGTTGCATTGAGGGTTA+GGG 0.426305 2_4:+19948559 None:intergenic
TTAAATAATTACATTGCAAA+TGG 0.430290 2_4:-19950410 Msa0319860:five_prime_UTR
AAATCAACAGTGTAATGAAA+AGG 0.433849 2_4:-19948155 None:intergenic
ATTTCCTTTATGTTGCATTG+AGG 0.445439 2_4:+19948552 None:intergenic
TAACCCTCAATGCAACATAA+AGG 0.456687 2_4:-19948556 Msa0319860:exon
CTAACTTACCAAAAGAAATC+AGG 0.463023 2_4:+19949288 None:intergenic
AATCAACAGTGTAATGAAAA+GGG 0.483652 2_4:-19948154 None:intergenic
ACGAGAGAGAAGACAAAGAA+TGG 0.483949 2_4:+19950226 None:intergenic
CCGTACTTTGGAAAATTGGC+AGG 0.487654 2_4:-19948218 Msa0319860:three_prime_UTR
ACACAAAATGCCATTCCAAT+GGG 0.496683 2_4:+19950449 None:intergenic
AACTGACACCATGGTGGTCG+CGG 0.498135 2_4:+19949322 None:intergenic
TTTAGAAAGAAGGTAAGAGA+GGG 0.510889 2_4:-19948367 Msa0319860:three_prime_UTR
AACACAAAATGCCATTCCAA+TGG 0.512389 2_4:+19950448 None:intergenic
GACCATTGTTTCAACCATAC+CGG 0.531792 2_4:-19950144 Msa0319860:CDS
AGACAAAGGACGAGAGAGAA+TGG 0.535740 2_4:+19950297 None:intergenic
GAAGAACAAAAGCAACCCAT+TGG 0.542287 2_4:-19950464 Msa0319860:five_prime_UTR
GAGAGAATGGTCTATGGAAA+GGG 0.544616 2_4:+19950310 None:intergenic
AGAGAGAATGGTCTATGGAA+AGG 0.545395 2_4:+19950309 None:intergenic
AGAAGATAGATATAAAGAGA+AGG 0.553185 2_4:-19948498 Msa0319860:three_prime_UTR
ACTCCAGATTGTATCAACTT+CGG 0.558219 2_4:-19949345 Msa0319860:intron
TTCCGGTATGGTTGAAACAA+TGG 0.559943 2_4:+19950142 None:intergenic
ATGGTGTCAGTTGCATATGT+TGG 0.570541 2_4:-19949311 Msa0319860:CDS
GGGTGAAGAGAAGATGATGG+TGG 0.573190 2_4:+19948579 None:intergenic
AAGAGACAGAGAAAATGAGA+AGG 0.573810 2_4:-19948418 Msa0319860:three_prime_UTR
ATGCACTACGAATTCCGGTA+TGG 0.581133 2_4:+19950130 None:intergenic
TTAGGGTGAAGAGAAGATGA+TGG 0.585438 2_4:+19948576 None:intergenic
ACAAAAGCAACCCATTGGAA+TGG 0.593229 2_4:-19950459 Msa0319860:five_prime_UTR
AACATATGCAACTGACACCA+TGG 0.604835 2_4:+19949313 None:intergenic
AACTTCGGCCGCGACCACCA+TGG 0.661944 2_4:-19949330 Msa0319860:CDS
GACAAAGGATGTGAAGACAA+AGG 0.667167 2_4:+19950283 None:intergenic
ATATGCAACTGACACCATGG+TGG 0.756118 2_4:+19949316 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAGAATTTGTTATA+TGG - chr2_4:19948902-19948921 Msa0319860:intron 10.0%
!!! AAATTTGAAATTTGAAATTT+TGG - chr2_4:19950327-19950346 Msa0319860:five_prime_UTR 10.0%
!!! TGTTGATTTAATTTATTTAA+GGG + chr2_4:19950486-19950505 None:intergenic 10.0%
!!! AAAAAAAAGAATTTGTTATA+TGG - chr2_4:19948902-19948921 Msa0319860:intron 10.0%
!!! AAATTTGAAATTTGAAATTT+TGG - chr2_4:19950327-19950346 Msa0319860:five_prime_UTR 10.0%
!!! TGTTGATTTAATTTATTTAA+GGG + chr2_4:19950486-19950505 None:intergenic 10.0%
!! TTAAATAATTACATTGCAAA+TGG - chr2_4:19948242-19948261 Msa0319860:three_prime_UTR 15.0%
!! AAAAAAAAATATGAAGACAA+AGG + chr2_4:19948387-19948406 None:intergenic 15.0%
!!! ATGAATCTGTTTATATTTTT+TGG - chr2_4:19948772-19948791 Msa0319860:intron 15.0%
!! GATATATATCTAAATTTGTA+TGG - chr2_4:19948826-19948845 Msa0319860:intron 15.0%
!!! AAGAAACTTTTTTTTTTCTT+TGG - chr2_4:19949145-19949164 Msa0319860:intron 15.0%
!!! TCTAAGTAATTCAATATTTT+AGG + chr2_4:19949589-19949608 None:intergenic 15.0%
!! AAATAAATCAATGTTACATT+TGG + chr2_4:19950405-19950424 None:intergenic 15.0%
!!! CTGTTGATTTAATTTATTTA+AGG + chr2_4:19950487-19950506 None:intergenic 15.0%
!! TTAAATAATTACATTGCAAA+TGG - chr2_4:19948242-19948261 Msa0319860:three_prime_UTR 15.0%
!! AAAAAAAAATATGAAGACAA+AGG + chr2_4:19948387-19948406 None:intergenic 15.0%
!!! ATGAATCTGTTTATATTTTT+TGG - chr2_4:19948772-19948791 Msa0319860:intron 15.0%
!! GATATATATCTAAATTTGTA+TGG - chr2_4:19948826-19948845 Msa0319860:intron 15.0%
!!! AAGAAACTTTTTTTTTTCTT+TGG - chr2_4:19949145-19949164 Msa0319860:intron 15.0%
!!! TCTAAGTAATTCAATATTTT+AGG + chr2_4:19949589-19949608 None:intergenic 15.0%
!! AAATAAATCAATGTTACATT+TGG + chr2_4:19950405-19950424 None:intergenic 15.0%
!!! CTGTTGATTTAATTTATTTA+AGG + chr2_4:19950487-19950506 None:intergenic 15.0%
!!! GATTAGATTAGATCTAATTT+TGG - chr2_4:19948658-19948677 Msa0319860:intron 20.0%
!!! TTTTCTGCAAAATAAATCAA+TGG - chr2_4:19949049-19949068 Msa0319860:intron 20.0%
!!! GCGTATTTTTTTTTATTTGT+TGG - chr2_4:19949171-19949190 Msa0319860:intron 20.0%
!!! ATATAAAGAATCTCTTTTGT+TGG - chr2_4:19949234-19949253 Msa0319860:intron 20.0%
!! TATCATTTATCAAAACATGA+AGG + chr2_4:19949548-19949567 None:intergenic 20.0%
!! ATATTGAATAACTTAGATTC+AGG - chr2_4:19949615-19949634 Msa0319860:intron 20.0%
!! TATTGAATAACTTAGATTCA+GGG - chr2_4:19949616-19949635 Msa0319860:intron 20.0%
!! ATCAAAATAAAAAGTCATCA+AGG + chr2_4:19950016-19950035 None:intergenic 20.0%
!! TTGATGAAGTTTAAGAAAAA+TGG - chr2_4:19950031-19950050 Msa0319860:intron 20.0%
!!! AATCTGTTTTTGAATTAAAG+TGG - chr2_4:19950372-19950391 Msa0319860:five_prime_UTR 20.0%
!!! GATTAGATTAGATCTAATTT+TGG - chr2_4:19948658-19948677 Msa0319860:intron 20.0%
!!! TTTTCTGCAAAATAAATCAA+TGG - chr2_4:19949049-19949068 Msa0319860:intron 20.0%
!!! GCGTATTTTTTTTTATTTGT+TGG - chr2_4:19949171-19949190 Msa0319860:intron 20.0%
!!! ATATAAAGAATCTCTTTTGT+TGG - chr2_4:19949234-19949253 Msa0319860:intron 20.0%
!! TATCATTTATCAAAACATGA+AGG + chr2_4:19949548-19949567 None:intergenic 20.0%
!! ATATTGAATAACTTAGATTC+AGG - chr2_4:19949615-19949634 Msa0319860:intron 20.0%
!! TATTGAATAACTTAGATTCA+GGG - chr2_4:19949616-19949635 Msa0319860:intron 20.0%
!! ATCAAAATAAAAAGTCATCA+AGG + chr2_4:19950016-19950035 None:intergenic 20.0%
!! TTGATGAAGTTTAAGAAAAA+TGG - chr2_4:19950031-19950050 Msa0319860:intron 20.0%
!!! AATCTGTTTTTGAATTAAAG+TGG - chr2_4:19950372-19950391 Msa0319860:five_prime_UTR 20.0%
!!! ATAGAAATGGATTGTGTTTT+TGG + chr2_4:19948469-19948488 None:intergenic 25.0%
! TAAGTGTGATAACATAGAAA+TGG + chr2_4:19948482-19948501 None:intergenic 25.0%
!!! TCTCTTTTGTTGGTTTTTTT+AGG - chr2_4:19949244-19949263 Msa0319860:intron 25.0%
!!! CTCTTTTGTTGGTTTTTTTA+GGG - chr2_4:19949245-19949264 Msa0319860:intron 25.0%
! ATTGAATAACTTAGATTCAG+GGG - chr2_4:19949617-19949636 Msa0319860:intron 25.0%
! AGAAGATAGATATAAAGAGA+AGG - chr2_4:19950154-19950173 Msa0319860:CDS 25.0%
!!! GTCTGTATTTTTTAGAAAGA+AGG - chr2_4:19950275-19950294 Msa0319860:five_prime_UTR 25.0%
!!! ATAGAAATGGATTGTGTTTT+TGG + chr2_4:19948469-19948488 None:intergenic 25.0%
! TAAGTGTGATAACATAGAAA+TGG + chr2_4:19948482-19948501 None:intergenic 25.0%
!!! TCTCTTTTGTTGGTTTTTTT+AGG - chr2_4:19949244-19949263 Msa0319860:intron 25.0%
!!! CTCTTTTGTTGGTTTTTTTA+GGG - chr2_4:19949245-19949264 Msa0319860:intron 25.0%
! ATTGAATAACTTAGATTCAG+GGG - chr2_4:19949617-19949636 Msa0319860:intron 25.0%
! AGAAGATAGATATAAAGAGA+AGG - chr2_4:19950154-19950173 Msa0319860:CDS 25.0%
!!! GTCTGTATTTTTTAGAAAGA+AGG - chr2_4:19950275-19950294 Msa0319860:five_prime_UTR 25.0%
!! GATTCATCTATTTCTGTTGT+TGG - chr2_4:19948547-19948566 Msa0319860:exon 30.0%
! CATTTGATTGTTGTTGTTGT+TGG + chr2_4:19948639-19948658 None:intergenic 30.0%
!!! GGATCTGTTATTTTTGTTGT+TGG - chr2_4:19948923-19948942 Msa0319860:intron 30.0%
GATGAAAATAGAGTTGTGTA+AGG - chr2_4:19949091-19949110 Msa0319860:intron 30.0%
AGTTGTGTAAGGAAAAAGAA+AGG - chr2_4:19949102-19949121 Msa0319860:intron 30.0%
CTAACTTACCAAAAGAAATC+AGG + chr2_4:19949367-19949386 None:intergenic 30.0%
TAGATCTGTGATAGATGTAA+CGG - chr2_4:19949403-19949422 Msa0319860:intron 30.0%
TAACGGCAACAATAAAAATC+TGG - chr2_4:19949420-19949439 Msa0319860:intron 30.0%
TTTCCTTTATGTTGCATTGA+GGG + chr2_4:19950102-19950121 None:intergenic 30.0%
ATTTCCTTTATGTTGCATTG+AGG + chr2_4:19950103-19950122 None:intergenic 30.0%
!! TTTTAGAAAGAAGGTAAGAG+AGG - chr2_4:19950284-19950303 Msa0319860:five_prime_UTR 30.0%
TTTAGAAAGAAGGTAAGAGA+GGG - chr2_4:19950285-19950304 Msa0319860:five_prime_UTR 30.0%
!! GATTCATCTATTTCTGTTGT+TGG - chr2_4:19948547-19948566 Msa0319860:exon 30.0%
! CATTTGATTGTTGTTGTTGT+TGG + chr2_4:19948639-19948658 None:intergenic 30.0%
!!! GGATCTGTTATTTTTGTTGT+TGG - chr2_4:19948923-19948942 Msa0319860:intron 30.0%
GATGAAAATAGAGTTGTGTA+AGG - chr2_4:19949091-19949110 Msa0319860:intron 30.0%
AGTTGTGTAAGGAAAAAGAA+AGG - chr2_4:19949102-19949121 Msa0319860:intron 30.0%
CTAACTTACCAAAAGAAATC+AGG + chr2_4:19949367-19949386 None:intergenic 30.0%
TAGATCTGTGATAGATGTAA+CGG - chr2_4:19949403-19949422 Msa0319860:intron 30.0%
TAACGGCAACAATAAAAATC+TGG - chr2_4:19949420-19949439 Msa0319860:intron 30.0%
TTTCCTTTATGTTGCATTGA+GGG + chr2_4:19950102-19950121 None:intergenic 30.0%
ATTTCCTTTATGTTGCATTG+AGG + chr2_4:19950103-19950122 None:intergenic 30.0%
!! TTTTAGAAAGAAGGTAAGAG+AGG - chr2_4:19950284-19950303 Msa0319860:five_prime_UTR 30.0%
TTTAGAAAGAAGGTAAGAGA+GGG - chr2_4:19950285-19950304 Msa0319860:five_prime_UTR 30.0%
ACACAAAATGCCATTCCAAT+GGG + chr2_4:19948206-19948225 None:intergenic 35.0%
AACACAAAATGCCATTCCAA+TGG + chr2_4:19948207-19948226 None:intergenic 35.0%
!! GTTTTTGGAAGAAGAAGTGT+GGG + chr2_4:19948454-19948473 None:intergenic 35.0%
!!! TGTTTTTGGAAGAAGAAGTG+TGG + chr2_4:19948455-19948474 None:intergenic 35.0%
TAAGCATTCATGTCATGATG+AGG - chr2_4:19948570-19948589 Msa0319860:CDS 35.0%
! ATGATGAGGACGAGATATTT+TGG - chr2_4:19948584-19948603 Msa0319860:CDS 35.0%
!!! AGATATTTTGGTCTCCACTA+CGG - chr2_4:19948596-19948615 Msa0319860:CDS 35.0%
ACTCCAGATTGTATCAACTT+CGG - chr2_4:19949307-19949326 Msa0319860:CDS 35.0%
!! TATGTTGGCCTGATTTCTTT+TGG - chr2_4:19949356-19949375 Msa0319860:intron 35.0%
CTTTATTGTGAGGTTTCTGT+TGG + chr2_4:19949858-19949877 None:intergenic 35.0%
CAACAGAAACCTCACAATAA+AGG - chr2_4:19949856-19949875 Msa0319860:intron 35.0%
AATAACACAGTTCTCATGTG+AGG + chr2_4:19949948-19949967 None:intergenic 35.0%
TTATGTTGCATTGAGGGTTA+GGG + chr2_4:19950096-19950115 None:intergenic 35.0%
TTTATGTTGCATTGAGGGTT+AGG + chr2_4:19950097-19950116 None:intergenic 35.0%
TAACCCTCAATGCAACATAA+AGG - chr2_4:19950096-19950115 Msa0319860:intron 35.0%
AAGAGACAGAGAAAATGAGA+AGG - chr2_4:19950234-19950253 Msa0319860:five_prime_UTR 35.0%
ACACAAAATGCCATTCCAAT+GGG + chr2_4:19948206-19948225 None:intergenic 35.0%
AACACAAAATGCCATTCCAA+TGG + chr2_4:19948207-19948226 None:intergenic 35.0%
!! GTTTTTGGAAGAAGAAGTGT+GGG + chr2_4:19948454-19948473 None:intergenic 35.0%
!!! TGTTTTTGGAAGAAGAAGTG+TGG + chr2_4:19948455-19948474 None:intergenic 35.0%
TAAGCATTCATGTCATGATG+AGG - chr2_4:19948570-19948589 Msa0319860:CDS 35.0%
! ATGATGAGGACGAGATATTT+TGG - chr2_4:19948584-19948603 Msa0319860:CDS 35.0%
!!! AGATATTTTGGTCTCCACTA+CGG - chr2_4:19948596-19948615 Msa0319860:CDS 35.0%
ACTCCAGATTGTATCAACTT+CGG - chr2_4:19949307-19949326 Msa0319860:CDS 35.0%
!! TATGTTGGCCTGATTTCTTT+TGG - chr2_4:19949356-19949375 Msa0319860:intron 35.0%
CTTTATTGTGAGGTTTCTGT+TGG + chr2_4:19949858-19949877 None:intergenic 35.0%
CAACAGAAACCTCACAATAA+AGG - chr2_4:19949856-19949875 Msa0319860:intron 35.0%
AATAACACAGTTCTCATGTG+AGG + chr2_4:19949948-19949967 None:intergenic 35.0%
TTATGTTGCATTGAGGGTTA+GGG + chr2_4:19950096-19950115 None:intergenic 35.0%
TTTATGTTGCATTGAGGGTT+AGG + chr2_4:19950097-19950116 None:intergenic 35.0%
TAACCCTCAATGCAACATAA+AGG - chr2_4:19950096-19950115 Msa0319860:intron 35.0%
AAGAGACAGAGAAAATGAGA+AGG - chr2_4:19950234-19950253 Msa0319860:five_prime_UTR 35.0%
GAAGAACAAAAGCAACCCAT+TGG - chr2_4:19948188-19948207 Msa0319860:three_prime_UTR 40.0%
ACAAAAGCAACCCATTGGAA+TGG - chr2_4:19948193-19948212 Msa0319860:three_prime_UTR 40.0%
! TGTGATGTGTCAGCTTTTGT+TGG + chr2_4:19948290-19948309 None:intergenic 40.0%
GAGAGAATGGTCTATGGAAA+GGG + chr2_4:19948345-19948364 None:intergenic 40.0%
AGAGAGAATGGTCTATGGAA+AGG + chr2_4:19948346-19948365 None:intergenic 40.0%
GACAAAGGATGTGAAGACAA+AGG + chr2_4:19948372-19948391 None:intergenic 40.0%
ACGAGAGAGAAGACAAAGAA+TGG + chr2_4:19948429-19948448 None:intergenic 40.0%
GACCATTGTTTCAACCATAC+CGG - chr2_4:19948508-19948527 Msa0319860:three_prime_UTR 40.0%
TTCCGGTATGGTTGAAACAA+TGG + chr2_4:19948513-19948532 None:intergenic 40.0%
ATCTGATGCACTACGAATTC+CGG + chr2_4:19948530-19948549 None:intergenic 40.0%
! TGTTTCAAGTATGACCGTAG+TGG + chr2_4:19948613-19948632 None:intergenic 40.0%
AACATATGCAACTGACACCA+TGG + chr2_4:19949342-19949361 None:intergenic 40.0%
! ATGGTGTCAGTTGCATATGT+TGG - chr2_4:19949341-19949360 Msa0319860:CDS 40.0%
GCTCATTGTCCTTTATTGTG+AGG + chr2_4:19949868-19949887 None:intergenic 40.0%
TTAGGGTGAAGAGAAGATGA+TGG + chr2_4:19950079-19950098 None:intergenic 40.0%
TTTATTCTCCCTCCGTACTT+TGG - chr2_4:19950422-19950441 Msa0319860:five_prime_UTR 40.0%
! CCAATTTTCCAAAGTACGGA+GGG + chr2_4:19950433-19950452 None:intergenic 40.0%
! CCTGCCAATTTTCCAAAGTA+CGG + chr2_4:19950437-19950456 None:intergenic 40.0%
GAAGAACAAAAGCAACCCAT+TGG - chr2_4:19948188-19948207 Msa0319860:three_prime_UTR 40.0%
ACAAAAGCAACCCATTGGAA+TGG - chr2_4:19948193-19948212 Msa0319860:three_prime_UTR 40.0%
! TGTGATGTGTCAGCTTTTGT+TGG + chr2_4:19948290-19948309 None:intergenic 40.0%
GAGAGAATGGTCTATGGAAA+GGG + chr2_4:19948345-19948364 None:intergenic 40.0%
AGAGAGAATGGTCTATGGAA+AGG + chr2_4:19948346-19948365 None:intergenic 40.0%
GACAAAGGATGTGAAGACAA+AGG + chr2_4:19948372-19948391 None:intergenic 40.0%
ACGAGAGAGAAGACAAAGAA+TGG + chr2_4:19948429-19948448 None:intergenic 40.0%
GACCATTGTTTCAACCATAC+CGG - chr2_4:19948508-19948527 Msa0319860:three_prime_UTR 40.0%
TTCCGGTATGGTTGAAACAA+TGG + chr2_4:19948513-19948532 None:intergenic 40.0%
ATCTGATGCACTACGAATTC+CGG + chr2_4:19948530-19948549 None:intergenic 40.0%
! TGTTTCAAGTATGACCGTAG+TGG + chr2_4:19948613-19948632 None:intergenic 40.0%
AACATATGCAACTGACACCA+TGG + chr2_4:19949342-19949361 None:intergenic 40.0%
! ATGGTGTCAGTTGCATATGT+TGG - chr2_4:19949341-19949360 Msa0319860:CDS 40.0%
GCTCATTGTCCTTTATTGTG+AGG + chr2_4:19949868-19949887 None:intergenic 40.0%
TTAGGGTGAAGAGAAGATGA+TGG + chr2_4:19950079-19950098 None:intergenic 40.0%
TTTATTCTCCCTCCGTACTT+TGG - chr2_4:19950422-19950441 Msa0319860:five_prime_UTR 40.0%
! CCAATTTTCCAAAGTACGGA+GGG + chr2_4:19950433-19950452 None:intergenic 40.0%
! CCTGCCAATTTTCCAAAGTA+CGG + chr2_4:19950437-19950456 None:intergenic 40.0%
AGACAAAGGACGAGAGAGAA+TGG + chr2_4:19948358-19948377 None:intergenic 45.0%
ATGCACTACGAATTCCGGTA+TGG + chr2_4:19948525-19948544 None:intergenic 45.0%
ATATGCAACTGACACCATGG+TGG + chr2_4:19949339-19949358 None:intergenic 45.0%
AACACAGTTCTCATGTGAGG+AGG + chr2_4:19949945-19949964 None:intergenic 45.0%
! CCCTCCGTACTTTGGAAAAT+TGG - chr2_4:19950430-19950449 Msa0319860:five_prime_UTR 45.0%
! GCCAATTTTCCAAAGTACGG+AGG + chr2_4:19950434-19950453 None:intergenic 45.0%
! CCGTACTTTGGAAAATTGGC+AGG - chr2_4:19950434-19950453 Msa0319860:five_prime_UTR 45.0%
AGACAAAGGACGAGAGAGAA+TGG + chr2_4:19948358-19948377 None:intergenic 45.0%
ATGCACTACGAATTCCGGTA+TGG + chr2_4:19948525-19948544 None:intergenic 45.0%
ATATGCAACTGACACCATGG+TGG + chr2_4:19949339-19949358 None:intergenic 45.0%
AACACAGTTCTCATGTGAGG+AGG + chr2_4:19949945-19949964 None:intergenic 45.0%
! CCCTCCGTACTTTGGAAAAT+TGG - chr2_4:19950430-19950449 Msa0319860:five_prime_UTR 45.0%
! GCCAATTTTCCAAAGTACGG+AGG + chr2_4:19950434-19950453 None:intergenic 45.0%
! CCGTACTTTGGAAAATTGGC+AGG - chr2_4:19950434-19950453 Msa0319860:five_prime_UTR 45.0%
GGACGAGAGAGAATGGTCTA+TGG + chr2_4:19948351-19948370 None:intergenic 50.0%
!! CGGCCGAAGTTGATACAATC+TGG + chr2_4:19949313-19949332 None:intergenic 50.0%
! GGGTGAAGAGAAGATGATGG+TGG + chr2_4:19950076-19950095 None:intergenic 50.0%
GGACGAGAGAGAATGGTCTA+TGG + chr2_4:19948351-19948370 None:intergenic 50.0%
!! CGGCCGAAGTTGATACAATC+TGG + chr2_4:19949313-19949332 None:intergenic 50.0%
! GGGTGAAGAGAAGATGATGG+TGG + chr2_4:19950076-19950095 None:intergenic 50.0%
AACTGACACCATGGTGGTCG+CGG + chr2_4:19949333-19949352 None:intergenic 55.0%
AACTGACACCATGGTGGTCG+CGG + chr2_4:19949333-19949352 None:intergenic 55.0%
AACTTCGGCCGCGACCACCA+TGG - chr2_4:19949322-19949341 Msa0319860:CDS 65.0%
AACTTCGGCCGCGACCACCA+TGG - chr2_4:19949322-19949341 Msa0319860:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 19948157 19950517 19948157 ID=Msa0319860;Name=Msa0319860
chr2_4 mRNA 19948157 19950517 19948157 ID=Msa0319860-mRNA-1;Parent=Msa0319860;Name=Msa0319860-mRNA-1;_AED=0.20;_eAED=0.20;_QI=330|1|1|1|0|0.33|3|402|73
chr2_4 exon 19948157 19948634 19948157 ID=Msa0319860-mRNA-1:exon:7365;Parent=Msa0319860-mRNA-1
chr2_4 exon 19949297 19949360 19949297 ID=Msa0319860-mRNA-1:exon:7364;Parent=Msa0319860-mRNA-1
chr2_4 exon 19950106 19950517 19950106 ID=Msa0319860-mRNA-1:exon:7363;Parent=Msa0319860-mRNA-1
chr2_4 five_prime_UTR 19950188 19950517 19950188 ID=Msa0319860-mRNA-1:five_prime_utr;Parent=Msa0319860-mRNA-1
chr2_4 CDS 19950106 19950187 19950106 ID=Msa0319860-mRNA-1:cds;Parent=Msa0319860-mRNA-1
chr2_4 CDS 19949297 19949360 19949297 ID=Msa0319860-mRNA-1:cds;Parent=Msa0319860-mRNA-1
chr2_4 CDS 19948559 19948634 19948559 ID=Msa0319860-mRNA-1:cds;Parent=Msa0319860-mRNA-1
chr2_4 three_prime_UTR 19948157 19948558 19948157 ID=Msa0319860-mRNA-1:three_prime_utr;Parent=Msa0319860-mRNA-1
Gene Sequence

>Msa0319860

ATGTTATCACACTTATTATGCGACCATTGTTTCAACCATACCGGAATTCGTAGTGCATCAGATTCATCTATTTCTGTTGTTGATTGTATCAACTTCGGCCGCGACCACCATGGTGTCAGTTGCATATGTTGGCCTGATTTCTTTTGTTTAAGAAAAATGGTTGAAGCTGCTCATTATTTCCACCATCATCTTCTCTTCACCCTAACCCTCAATGCAACATAA
Protein sequence

>Msa0319860

MLSHLLCDHCFNHTGIRSASDSSISVVDCINFGRDHHGVSCICWPDFFCLRKMVEAAHYFHHHLLFTLTLNAT*