AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0927520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0927520 MtrunA17_Chr8g0381591 84.211 57 9 0 3 59 430 486 2.99e-26 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0927520 AT1G67840.1 53.448 58 27 0 2 59 435 492 3.13e-13 62.4

Find 51 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGAATGACTTCATATGATA+TGG 0.227148 6_2:-71878404 Msa0927520:five_prime_UTR
CAATTCGTAACCCTGTATTT+TGG 0.228001 6_2:-71878345 Msa0927520:five_prime_UTR
TTGATACTTCTTACAATCTA+TGG 0.245913 6_2:-71879496 Msa0927520:five_prime_UTR
TCCAAACAAAATTTAATATA+AGG 0.256220 6_2:+71879365 None:intergenic
AATATGATTTATCTAATTCT+TGG 0.268932 6_2:-71879552 Msa0927520:five_prime_UTR
CTGGTAAGCAACAGCGAAAA+AGG 0.295430 6_2:+71878889 None:intergenic
ATGGATCATTTAGCTATATA+TGG 0.295571 6_2:-71879472 Msa0927520:five_prime_UTR
CCTCTTGTGTGTGTTGAAAA+TGG 0.354236 6_2:-71878375 Msa0927520:five_prime_UTR
AACACCAACTATTAAATTCT+CGG 0.366167 6_2:-71879395 Msa0927520:five_prime_UTR
GTGATACCTGTTGTGCCATT+TGG 0.390693 6_2:-71878945 Msa0927520:five_prime_UTR
CATGCATAGCGCCAAAATAC+AGG 0.392919 6_2:+71878334 Msa0927510:three_prime_UTR
ACCAGATGAGCTGCTCTTTG+AGG 0.392938 6_2:-71878871 Msa0927520:intron
ACCTCAAAGAGCAGCTCATC+TGG 0.404585 6_2:+71878870 None:intergenic
CTGAAAGCCTGGCGCAGAGC+AGG 0.408438 6_2:+71877310 Msa0927510:intron
TTGGCGCTATGCATGCTTGA+AGG 0.410618 6_2:-71878326 Msa0927520:exon
TTTAGCTATATATGGTTGTC+TGG 0.414469 6_2:-71879464 Msa0927520:five_prime_UTR
TTTATGCATACTTAAATTGA+AGG 0.418138 6_2:-71879295 Msa0927520:five_prime_UTR
CTGTTAACTACTTTGTTACA+AGG 0.425322 6_2:-71879096 Msa0927520:five_prime_UTR
AACCAAGTCTGCCAATACTT+TGG 0.432850 6_2:+71877420 Msa0927510:intron
GGAGTAAAGCACCTTCAATA+AGG 0.437993 6_2:+71877284 Msa0927510:intron
TTCCAAAGTATTGGCAGACT+TGG 0.442728 6_2:-71877422 Msa0927520:CDS
GCACTTCCAAATGGCACAAC+AGG 0.444249 6_2:+71878939 None:intergenic
GCTTTCAGCAACCTTATTGA+AGG 0.452369 6_2:-71877295 None:intergenic
CAATAAGGTTGCTGAAAGCC+TGG 0.458375 6_2:+71877299 Msa0927510:intron
TTTAACGGTCCCTTCTGAGT+TGG 0.465255 6_2:-71879140 Msa0927520:five_prime_UTR
CGAATGACTTCATATGATAT+GGG 0.471301 6_2:-71878403 Msa0927520:five_prime_UTR
TTTCTACTTGCACTTCCAAA+TGG 0.473755 6_2:+71878930 None:intergenic
GGTCCCTTCTGAGTTGGAAT+CGG 0.480778 6_2:-71879134 Msa0927520:five_prime_UTR
ACAAAATTTAATATAAGGAG+AGG 0.481991 6_2:+71879370 None:intergenic
ACTTCTTACAATCTATGGTA+TGG 0.503413 6_2:-71879491 Msa0927520:five_prime_UTR
TGCATGCTTGAAGGGTTGGC+AGG 0.511193 6_2:-71878317 Msa0927520:intron
AGAAGAACCTGCTCTGCGCC+AGG 0.520287 6_2:-71877317 Msa0927520:CDS
TATTTCACAGATTATTTAAG+CGG 0.532188 6_2:-71879265 Msa0927520:five_prime_UTR
TGGCGCTATGCATGCTTGAA+GGG 0.539052 6_2:-71878325 Msa0927520:exon
GGGTCCGAGAATTTAATAGT+TGG 0.543936 6_2:+71879391 None:intergenic
GCTATGCATGCTTGAAGGGT+TGG 0.559505 6_2:-71878321 Msa0927520:exon
GTTACAAGGAGGCCATGGCT+TGG 0.560559 6_2:-71879082 Msa0927520:intron
ACTTTGTTACAAGGAGGCCA+TGG 0.571990 6_2:-71879087 Msa0927520:five_prime_UTR
TTGATTCATGAATCATACCT+AGG 0.590307 6_2:-71879209 Msa0927520:five_prime_UTR
AAACCGATTCCAACTCAGAA+GGG 0.600214 6_2:+71879131 None:intergenic
AAAACCGATTCCAACTCAGA+AGG 0.609545 6_2:+71879130 None:intergenic
TCTTCTACAGCAGCTAGCAA+AGG 0.616929 6_2:+71877334 Msa0927510:intron
ATGCATAGCGCCAAAATACA+GGG 0.620193 6_2:+71878335 Msa0927510:three_prime_UTR
CACAGTATACAACTTTCAGT+AGG 0.621325 6_2:-71879581 Msa0927520:five_prime_UTR
CAAAATTTAATATAAGGAGA+GGG 0.621758 6_2:+71879371 None:intergenic
TTTGGTTAAAGATCTTTATG+GGG 0.625965 6_2:-71878973 Msa0927520:intron
AGATGTTAATGAAAGAACCT+AGG 0.640089 6_2:+71879192 None:intergenic
GCGTTGTACATACCAAGCCA+TGG 0.657436 6_2:+71879070 None:intergenic
TTAACTACTTTGTTACAAGG+AGG 0.664306 6_2:-71879093 Msa0927520:five_prime_UTR
AAAAGGGAAGAATCAACATG+AGG 0.682862 6_2:+71878906 None:intergenic
CTTGTGTGTGTTGAAAATGG+CGG 0.786516 6_2:-71878372 Msa0927520:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAAATATTACCTTT+AGG - chr6_2:71879301-71879320 Msa0927520:five_prime_UTR 10.0%
!! TAAAAAAAATATTACCTTTA+GGG - chr6_2:71879302-71879321 Msa0927520:five_prime_UTR 10.0%
!! ATAAAAAAAATATTACCTTT+AGG - chr6_2:71879301-71879320 Msa0927520:five_prime_UTR 10.0%
!! TAAAAAAAATATTACCTTTA+GGG - chr6_2:71879302-71879321 Msa0927520:five_prime_UTR 10.0%
!! AATATGATTTATCTAATTCT+TGG - chr6_2:71877328-71877347 Msa0927520:CDS 15.0%
!!! TTCTGTTCTAATTTTATAAA+AGG - chr6_2:71877445-71877464 Msa0927520:intron 15.0%
!!! TCTGTTCTAATTTTATAAAA+GGG - chr6_2:71877446-71877465 Msa0927520:intron 15.0%
!!! TCCTTATATTAAATTTTGTT+TGG - chr6_2:71877514-71877533 Msa0927520:intron 15.0%
!! TCCAAACAAAATTTAATATA+AGG + chr6_2:71877518-71877537 Msa0927510:intron 15.0%
!!! ATATATTTTTGATGCAAAAT+TGG + chr6_2:71879283-71879302 None:intergenic 15.0%
!! AATATGATTTATCTAATTCT+TGG - chr6_2:71877328-71877347 Msa0927520:CDS 15.0%
!!! TTCTGTTCTAATTTTATAAA+AGG - chr6_2:71877445-71877464 Msa0927520:intron 15.0%
!!! TCTGTTCTAATTTTATAAAA+GGG - chr6_2:71877446-71877465 Msa0927520:intron 15.0%
!!! TCCTTATATTAAATTTTGTT+TGG - chr6_2:71877514-71877533 Msa0927520:intron 15.0%
!! TCCAAACAAAATTTAATATA+AGG + chr6_2:71877518-71877537 Msa0927510:intron 15.0%
!!! ATATATTTTTGATGCAAAAT+TGG + chr6_2:71879283-71879302 None:intergenic 15.0%
!!! CTGTTCTAATTTTATAAAAG+GGG - chr6_2:71877447-71877466 Msa0927520:intron 20.0%
!!! TGTTCTAATTTTATAAAAGG+GGG - chr6_2:71877448-71877467 Msa0927520:intron 20.0%
!! CAAAATTTAATATAAGGAGA+GGG + chr6_2:71877512-71877531 Msa0927510:intron 20.0%
!! ACAAAATTTAATATAAGGAG+AGG + chr6_2:71877513-71877532 Msa0927510:intron 20.0%
!! TTTATGCATACTTAAATTGA+AGG - chr6_2:71877585-71877604 Msa0927520:intron 20.0%
!! TATTTCACAGATTATTTAAG+CGG - chr6_2:71877615-71877634 Msa0927520:intron 20.0%
!!! GTTTGTAGATTTTTCTTTAA+CGG - chr6_2:71877725-71877744 Msa0927520:intron 20.0%
!!! CTTATAGTTTTTATGTGTTT+TGG - chr6_2:71877889-71877908 Msa0927520:intron 20.0%
!!! TTTTGGTTAAAGATCTTTAT+GGG - chr6_2:71877906-71877925 Msa0927520:intron 20.0%
!! ATATATGTAACTACACTATT+TGG - chr6_2:71878048-71878067 Msa0927520:intron 20.0%
!! TCTTAAAATAAGAAACAGAA+AGG - chr6_2:71878372-71878391 Msa0927520:five_prime_UTR 20.0%
!!! TTGGACAAATTTTTTACATA+GGG + chr6_2:71878911-71878930 None:intergenic 20.0%
!!! ATTGGACAAATTTTTTACAT+AGG + chr6_2:71878912-71878931 None:intergenic 20.0%
!! GTTAATAAAATGCATAAATC+TGG - chr6_2:71879057-71879076 Msa0927520:intron 20.0%
!!! CTGTTCTAATTTTATAAAAG+GGG - chr6_2:71877447-71877466 Msa0927520:intron 20.0%
!!! TGTTCTAATTTTATAAAAGG+GGG - chr6_2:71877448-71877467 Msa0927520:intron 20.0%
!! CAAAATTTAATATAAGGAGA+GGG + chr6_2:71877512-71877531 Msa0927510:intron 20.0%
!! ACAAAATTTAATATAAGGAG+AGG + chr6_2:71877513-71877532 Msa0927510:intron 20.0%
!! TTTATGCATACTTAAATTGA+AGG - chr6_2:71877585-71877604 Msa0927520:intron 20.0%
!! TATTTCACAGATTATTTAAG+CGG - chr6_2:71877615-71877634 Msa0927520:intron 20.0%
!!! GTTTGTAGATTTTTCTTTAA+CGG - chr6_2:71877725-71877744 Msa0927520:intron 20.0%
!!! CTTATAGTTTTTATGTGTTT+TGG - chr6_2:71877889-71877908 Msa0927520:intron 20.0%
!!! TTTTGGTTAAAGATCTTTAT+GGG - chr6_2:71877906-71877925 Msa0927520:intron 20.0%
!! ATATATGTAACTACACTATT+TGG - chr6_2:71878048-71878067 Msa0927520:intron 20.0%
!! TCTTAAAATAAGAAACAGAA+AGG - chr6_2:71878372-71878391 Msa0927520:five_prime_UTR 20.0%
!!! TTGGACAAATTTTTTACATA+GGG + chr6_2:71878911-71878930 None:intergenic 20.0%
!!! ATTGGACAAATTTTTTACAT+AGG + chr6_2:71878912-71878931 None:intergenic 20.0%
!! GTTAATAAAATGCATAAATC+TGG - chr6_2:71879057-71879076 Msa0927520:intron 20.0%
! TTGATACTTCTTACAATCTA+TGG - chr6_2:71877384-71877403 Msa0927520:CDS 25.0%
! ATGGATCATTTAGCTATATA+TGG - chr6_2:71877408-71877427 Msa0927520:CDS 25.0%
!! GTTCTAATTTTATAAAAGGG+GGG - chr6_2:71877449-71877468 Msa0927520:intron 25.0%
! AACACCAACTATTAAATTCT+CGG - chr6_2:71877485-71877504 Msa0927520:intron 25.0%
!!! GTTTTGGTTAAAGATCTTTA+TGG - chr6_2:71877905-71877924 Msa0927520:intron 25.0%
! TTTGGTTAAAGATCTTTATG+GGG - chr6_2:71877907-71877926 Msa0927520:intron 25.0%
! TACACTTGAAACTTAAAAGA+AGG - chr6_2:71878235-71878254 Msa0927520:intron 25.0%
! ATAAGAAACAGAAAGGTTTA+TGG - chr6_2:71878379-71878398 Msa0927520:five_prime_UTR 25.0%
! ATACTTCAATCAAAATACGA+CGG - chr6_2:71878692-71878711 Msa0927520:intron 25.0%
! ATAAACAATTCAGCTAATGT+GGG + chr6_2:71878866-71878885 None:intergenic 25.0%
! AAAATTTGTCCAATATATGC+AGG - chr6_2:71878918-71878937 Msa0927520:five_prime_UTR 25.0%
! GAATATTATCCTGCATATAT+TGG + chr6_2:71878930-71878949 None:intergenic 25.0%
! GAATCAATCAAGAAAATGAA+TGG - chr6_2:71878964-71878983 Msa0927520:five_prime_UTR 25.0%
! GAAAATATCTCTTTCGATAA+TGG - chr6_2:71879190-71879209 Msa0927520:five_prime_UTR 25.0%
! AATTTATAGGACTTCAGAAT+TGG + chr6_2:71879260-71879279 None:intergenic 25.0%
! GATGCAAAATTGGAATTTAT+AGG + chr6_2:71879273-71879292 None:intergenic 25.0%
! TTGATACTTCTTACAATCTA+TGG - chr6_2:71877384-71877403 Msa0927520:CDS 25.0%
! ATGGATCATTTAGCTATATA+TGG - chr6_2:71877408-71877427 Msa0927520:CDS 25.0%
!! GTTCTAATTTTATAAAAGGG+GGG - chr6_2:71877449-71877468 Msa0927520:intron 25.0%
! AACACCAACTATTAAATTCT+CGG - chr6_2:71877485-71877504 Msa0927520:intron 25.0%
!!! GTTTTGGTTAAAGATCTTTA+TGG - chr6_2:71877905-71877924 Msa0927520:intron 25.0%
! TTTGGTTAAAGATCTTTATG+GGG - chr6_2:71877907-71877926 Msa0927520:intron 25.0%
! TACACTTGAAACTTAAAAGA+AGG - chr6_2:71878235-71878254 Msa0927520:intron 25.0%
! ATAAGAAACAGAAAGGTTTA+TGG - chr6_2:71878379-71878398 Msa0927520:five_prime_UTR 25.0%
! ATACTTCAATCAAAATACGA+CGG - chr6_2:71878692-71878711 Msa0927520:intron 25.0%
! ATAAACAATTCAGCTAATGT+GGG + chr6_2:71878866-71878885 None:intergenic 25.0%
! AAAATTTGTCCAATATATGC+AGG - chr6_2:71878918-71878937 Msa0927520:five_prime_UTR 25.0%
! GAATATTATCCTGCATATAT+TGG + chr6_2:71878930-71878949 None:intergenic 25.0%
! GAATCAATCAAGAAAATGAA+TGG - chr6_2:71878964-71878983 Msa0927520:five_prime_UTR 25.0%
! GAAAATATCTCTTTCGATAA+TGG - chr6_2:71879190-71879209 Msa0927520:five_prime_UTR 25.0%
! AATTTATAGGACTTCAGAAT+TGG + chr6_2:71879260-71879279 None:intergenic 25.0%
! GATGCAAAATTGGAATTTAT+AGG + chr6_2:71879273-71879292 None:intergenic 25.0%
ACTTCTTACAATCTATGGTA+TGG - chr6_2:71877389-71877408 Msa0927520:CDS 30.0%
TTTAGCTATATATGGTTGTC+TGG - chr6_2:71877416-71877435 Msa0927520:CDS 30.0%
TTGATTCATGAATCATACCT+AGG - chr6_2:71877671-71877690 Msa0927520:intron 30.0%
AGATGTTAATGAAAGAACCT+AGG + chr6_2:71877691-71877710 Msa0927510:three_prime_UTR 30.0%
CTGTTAACTACTTTGTTACA+AGG - chr6_2:71877784-71877803 Msa0927520:intron 30.0%
TTAACTACTTTGTTACAAGG+AGG - chr6_2:71877787-71877806 Msa0927520:intron 30.0%
AGCACAATAGTAAATAACCA+TGG + chr6_2:71877845-71877864 Msa0927510:three_prime_UTR 30.0%
! TTGGTCATGACTTTTTGATA+TGG - chr6_2:71878067-71878086 Msa0927520:intron 30.0%
! CACAACTTATTGATGGTATT+TGG - chr6_2:71878307-71878326 Msa0927520:intron 30.0%
!! TGCATTATCGTTGATTCTAA+CGG - chr6_2:71878330-71878349 Msa0927520:exon 30.0%
ATTTATTGAATACACCTTGC+AGG - chr6_2:71878446-71878465 Msa0927520:intron 30.0%
TCGAATGACTTCATATGATA+TGG - chr6_2:71878476-71878495 Msa0927520:intron 30.0%
CGAATGACTTCATATGATAT+GGG - chr6_2:71878477-71878496 Msa0927520:intron 30.0%
AATATGAAGAGCAGGAAATA+AGG + chr6_2:71878793-71878812 None:intergenic 30.0%
CATAAACAATTCAGCTAATG+TGG + chr6_2:71878867-71878886 None:intergenic 30.0%
CACATTAGCTGAATTGTTTA+TGG - chr6_2:71878865-71878884 Msa0927520:intron 30.0%
ACATATATACACATTGAAGG+TGG + chr6_2:71879228-71879247 None:intergenic 30.0%
GGAACATATATACACATTGA+AGG + chr6_2:71879231-71879250 None:intergenic 30.0%
TCAAAAGGAAAATACCCTAA+AGG + chr6_2:71879319-71879338 None:intergenic 30.0%
!! TTTAGGGTATTTTCCTTTTG+AGG - chr6_2:71879318-71879337 Msa0927520:five_prime_UTR 30.0%
! GTCTAAAACTAAACCTCAAA+AGG + chr6_2:71879334-71879353 None:intergenic 30.0%
! CTGCAATTTTTCCAAAGTAT+TGG - chr6_2:71879449-71879468 Msa0927520:five_prime_UTR 30.0%
ACTTCTTACAATCTATGGTA+TGG - chr6_2:71877389-71877408 Msa0927520:CDS 30.0%
TTTAGCTATATATGGTTGTC+TGG - chr6_2:71877416-71877435 Msa0927520:CDS 30.0%
TTGATTCATGAATCATACCT+AGG - chr6_2:71877671-71877690 Msa0927520:intron 30.0%
AGATGTTAATGAAAGAACCT+AGG + chr6_2:71877691-71877710 Msa0927510:three_prime_UTR 30.0%
CTGTTAACTACTTTGTTACA+AGG - chr6_2:71877784-71877803 Msa0927520:intron 30.0%
TTAACTACTTTGTTACAAGG+AGG - chr6_2:71877787-71877806 Msa0927520:intron 30.0%
AGCACAATAGTAAATAACCA+TGG + chr6_2:71877845-71877864 Msa0927510:three_prime_UTR 30.0%
! TTGGTCATGACTTTTTGATA+TGG - chr6_2:71878067-71878086 Msa0927520:intron 30.0%
! CACAACTTATTGATGGTATT+TGG - chr6_2:71878307-71878326 Msa0927520:intron 30.0%
!! TGCATTATCGTTGATTCTAA+CGG - chr6_2:71878330-71878349 Msa0927520:exon 30.0%
ATTTATTGAATACACCTTGC+AGG - chr6_2:71878446-71878465 Msa0927520:intron 30.0%
TCGAATGACTTCATATGATA+TGG - chr6_2:71878476-71878495 Msa0927520:intron 30.0%
CGAATGACTTCATATGATAT+GGG - chr6_2:71878477-71878496 Msa0927520:intron 30.0%
AATATGAAGAGCAGGAAATA+AGG + chr6_2:71878793-71878812 None:intergenic 30.0%
CATAAACAATTCAGCTAATG+TGG + chr6_2:71878867-71878886 None:intergenic 30.0%
CACATTAGCTGAATTGTTTA+TGG - chr6_2:71878865-71878884 Msa0927520:intron 30.0%
ACATATATACACATTGAAGG+TGG + chr6_2:71879228-71879247 None:intergenic 30.0%
GGAACATATATACACATTGA+AGG + chr6_2:71879231-71879250 None:intergenic 30.0%
TCAAAAGGAAAATACCCTAA+AGG + chr6_2:71879319-71879338 None:intergenic 30.0%
!! TTTAGGGTATTTTCCTTTTG+AGG - chr6_2:71879318-71879337 Msa0927520:five_prime_UTR 30.0%
! GTCTAAAACTAAACCTCAAA+AGG + chr6_2:71879334-71879353 None:intergenic 30.0%
! CTGCAATTTTTCCAAAGTAT+TGG - chr6_2:71879449-71879468 Msa0927520:five_prime_UTR 30.0%
CACAGTATACAACTTTCAGT+AGG - chr6_2:71877299-71877318 Msa0927520:CDS 35.0%
TTTCTACTTGCACTTCCAAA+TGG + chr6_2:71877953-71877972 Msa0927510:intron 35.0%
AAAAGGGAAGAATCAACATG+AGG + chr6_2:71877977-71877996 Msa0927510:intron 35.0%
GATAACTCATGTGCGATATT+TGG - chr6_2:71878153-71878172 Msa0927520:intron 35.0%
TCACTTGCACAACTTATTGA+TGG - chr6_2:71878300-71878319 Msa0927520:intron 35.0%
! CAATTCGTAACCCTGTATTT+TGG - chr6_2:71878535-71878554 Msa0927520:intron 35.0%
!!! GTCATTGGAGGTTTTGAATA+TGG - chr6_2:71878590-71878609 Msa0927520:intron 35.0%
! AGTCTTTTTATGAAGCTCTC+TGG - chr6_2:71878650-71878669 Msa0927520:intron 35.0%
GAGTTAGACACACTATACTA+AGG + chr6_2:71878825-71878844 None:intergenic 35.0%
TAACTCTATGCGTAAATACG+AGG - chr6_2:71878839-71878858 Msa0927520:intron 35.0%
GATAGTTCTCAACTGTTAGA+TGG - chr6_2:71879030-71879049 Msa0927520:intron 35.0%
CACAGTATACAACTTTCAGT+AGG - chr6_2:71877299-71877318 Msa0927520:CDS 35.0%
TTTCTACTTGCACTTCCAAA+TGG + chr6_2:71877953-71877972 Msa0927510:intron 35.0%
AAAAGGGAAGAATCAACATG+AGG + chr6_2:71877977-71877996 Msa0927510:intron 35.0%
GATAACTCATGTGCGATATT+TGG - chr6_2:71878153-71878172 Msa0927520:intron 35.0%
TCACTTGCACAACTTATTGA+TGG - chr6_2:71878300-71878319 Msa0927520:intron 35.0%
! CAATTCGTAACCCTGTATTT+TGG - chr6_2:71878535-71878554 Msa0927520:intron 35.0%
!!! GTCATTGGAGGTTTTGAATA+TGG - chr6_2:71878590-71878609 Msa0927520:intron 35.0%
! AGTCTTTTTATGAAGCTCTC+TGG - chr6_2:71878650-71878669 Msa0927520:intron 35.0%
GAGTTAGACACACTATACTA+AGG + chr6_2:71878825-71878844 None:intergenic 35.0%
TAACTCTATGCGTAAATACG+AGG - chr6_2:71878839-71878858 Msa0927520:intron 35.0%
GATAGTTCTCAACTGTTAGA+TGG - chr6_2:71879030-71879049 Msa0927520:intron 35.0%
GGGTCCGAGAATTTAATAGT+TGG + chr6_2:71877492-71877511 Msa0927510:intron 40.0%
! AAACCGATTCCAACTCAGAA+GGG + chr6_2:71877752-71877771 Msa0927510:three_prime_UTR 40.0%
! AAAACCGATTCCAACTCAGA+AGG + chr6_2:71877753-71877772 Msa0927510:three_prime_UTR 40.0%
TGGTAAGCAACAGCGAAAAA+GGG + chr6_2:71877993-71878012 Msa0927510:intron 40.0%
GTGAGACCAGTTATAATGTG+CGG - chr6_2:71878091-71878110 Msa0927520:intron 40.0%
TGAGACCAGTTATAATGTGC+GGG - chr6_2:71878092-71878111 Msa0927520:intron 40.0%
TTATAATGTGCGGGAGTAGT+TGG - chr6_2:71878101-71878120 Msa0927520:intron 40.0%
! GCACATGAGTTATCTGAAGT+AGG + chr6_2:71878147-71878166 Msa0927510:three_prime_UTR 40.0%
!! TCATGTGCGATATTTGGTGT+GGG - chr6_2:71878159-71878178 Msa0927520:intron 40.0%
!! ATATTTGGTGTGGGTCTGTT+TGG - chr6_2:71878168-71878187 Msa0927520:intron 40.0%
! CCATTTTCAACACACACAAG+AGG + chr6_2:71878508-71878527 None:intergenic 40.0%
CCTCTTGTGTGTGTTGAAAA+TGG - chr6_2:71878505-71878524 Msa0927520:intron 40.0%
CTTGTGTGTGTTGAAAATGG+CGG - chr6_2:71878508-71878527 Msa0927520:intron 40.0%
ATGCATAGCGCCAAAATACA+GGG + chr6_2:71878548-71878567 None:intergenic 40.0%
!! TTTTATGAAGCTCTCTGGAG+CGG - chr6_2:71878655-71878674 Msa0927520:intron 40.0%
AATCAAAATACGACGGCTCA+TGG - chr6_2:71878699-71878718 Msa0927520:intron 40.0%
TTCCTGCTCTTCATATTGAC+TGG - chr6_2:71878796-71878815 Msa0927520:intron 40.0%
AACCAGTCAATATGAAGAGC+AGG + chr6_2:71878801-71878820 None:intergenic 40.0%
! GCAGTAACACTAAGAGTATG+TGG + chr6_2:71879087-71879106 None:intergenic 40.0%
TACCTGATTCCATGTCATAG+TGG + chr6_2:71879150-71879169 None:intergenic 40.0%
ACTATGACATGGAATCAGGT+AGG - chr6_2:71879149-71879168 Msa0927520:five_prime_UTR 40.0%
!! TTCCAAAGTATTGGCAGACT+TGG - chr6_2:71879458-71879477 Msa0927520:five_prime_UTR 40.0%
AACCAAGTCTGCCAATACTT+TGG + chr6_2:71879463-71879482 None:intergenic 40.0%
!! CGTTTTTGATTCTGAGCAAG+AGG + chr6_2:71879498-71879517 None:intergenic 40.0%
GGGTCCGAGAATTTAATAGT+TGG + chr6_2:71877492-71877511 Msa0927510:intron 40.0%
! AAACCGATTCCAACTCAGAA+GGG + chr6_2:71877752-71877771 Msa0927510:three_prime_UTR 40.0%
! AAAACCGATTCCAACTCAGA+AGG + chr6_2:71877753-71877772 Msa0927510:three_prime_UTR 40.0%
TGGTAAGCAACAGCGAAAAA+GGG + chr6_2:71877993-71878012 Msa0927510:intron 40.0%
GTGAGACCAGTTATAATGTG+CGG - chr6_2:71878091-71878110 Msa0927520:intron 40.0%
TGAGACCAGTTATAATGTGC+GGG - chr6_2:71878092-71878111 Msa0927520:intron 40.0%
TTATAATGTGCGGGAGTAGT+TGG - chr6_2:71878101-71878120 Msa0927520:intron 40.0%
! GCACATGAGTTATCTGAAGT+AGG + chr6_2:71878147-71878166 Msa0927510:three_prime_UTR 40.0%
!! TCATGTGCGATATTTGGTGT+GGG - chr6_2:71878159-71878178 Msa0927520:intron 40.0%
!! ATATTTGGTGTGGGTCTGTT+TGG - chr6_2:71878168-71878187 Msa0927520:intron 40.0%
! CCATTTTCAACACACACAAG+AGG + chr6_2:71878508-71878527 None:intergenic 40.0%
CCTCTTGTGTGTGTTGAAAA+TGG - chr6_2:71878505-71878524 Msa0927520:intron 40.0%
CTTGTGTGTGTTGAAAATGG+CGG - chr6_2:71878508-71878527 Msa0927520:intron 40.0%
ATGCATAGCGCCAAAATACA+GGG + chr6_2:71878548-71878567 None:intergenic 40.0%
!! TTTTATGAAGCTCTCTGGAG+CGG - chr6_2:71878655-71878674 Msa0927520:intron 40.0%
AATCAAAATACGACGGCTCA+TGG - chr6_2:71878699-71878718 Msa0927520:intron 40.0%
TTCCTGCTCTTCATATTGAC+TGG - chr6_2:71878796-71878815 Msa0927520:intron 40.0%
AACCAGTCAATATGAAGAGC+AGG + chr6_2:71878801-71878820 None:intergenic 40.0%
! GCAGTAACACTAAGAGTATG+TGG + chr6_2:71879087-71879106 None:intergenic 40.0%
TACCTGATTCCATGTCATAG+TGG + chr6_2:71879150-71879169 None:intergenic 40.0%
ACTATGACATGGAATCAGGT+AGG - chr6_2:71879149-71879168 Msa0927520:five_prime_UTR 40.0%
!! TTCCAAAGTATTGGCAGACT+TGG - chr6_2:71879458-71879477 Msa0927520:five_prime_UTR 40.0%
AACCAAGTCTGCCAATACTT+TGG + chr6_2:71879463-71879482 None:intergenic 40.0%
!! CGTTTTTGATTCTGAGCAAG+AGG + chr6_2:71879498-71879517 None:intergenic 40.0%
TTTAACGGTCCCTTCTGAGT+TGG - chr6_2:71877740-71877759 Msa0927520:intron 45.0%
!! CTGAGTTGGAATCGGTTTTG+AGG - chr6_2:71877754-71877773 Msa0927520:intron 45.0%
ACTTTGTTACAAGGAGGCCA+TGG - chr6_2:71877793-71877812 Msa0927520:intron 45.0%
! GTGATACCTGTTGTGCCATT+TGG - chr6_2:71877935-71877954 Msa0927520:intron 45.0%
CTGGTAAGCAACAGCGAAAA+AGG + chr6_2:71877994-71878013 Msa0927510:intron 45.0%
CTACTCCCGCACATTATAAC+TGG + chr6_2:71878100-71878119 Msa0927510:intron 45.0%
!! GGGAGTAGTTGGTTTGTTTG+CGG - chr6_2:71878112-71878131 Msa0927520:intron 45.0%
! CTCATGTGCGATATTTGGTG+TGG - chr6_2:71878158-71878177 Msa0927520:intron 45.0%
CATTCGAGCAAATACCTGCA+AGG + chr6_2:71878463-71878482 None:intergenic 45.0%
CATGCATAGCGCCAAAATAC+AGG + chr6_2:71878549-71878568 None:intergenic 45.0%
TTGGCAGGTAATGTCATTGG+AGG - chr6_2:71878578-71878597 Msa0927520:intron 45.0%
TTACCGTCAAATCGCAACCT+TGG + chr6_2:71878746-71878765 None:intergenic 45.0%
! TACTCTTAGTGTTACTGCCC+AGG - chr6_2:71879089-71879108 Msa0927520:five_prime_UTR 45.0%
!! AAGGGGCATCTCTATATCCT+GGG + chr6_2:71879109-71879128 None:intergenic 45.0%
AGTGAGAGCTAAAGGTGGAA+GGG + chr6_2:71879127-71879146 None:intergenic 45.0%
! CATAGTGGAGTGAGAGCTAA+AGG + chr6_2:71879135-71879154 None:intergenic 45.0%
CTCCACTATGACATGGAATC+AGG - chr6_2:71879145-71879164 Msa0927520:five_prime_UTR 45.0%
GGACTTCAGAATTGGCAGAA+TGG + chr6_2:71879252-71879271 None:intergenic 45.0%
TCTTCTACAGCAGCTAGCAA+AGG + chr6_2:71879549-71879568 None:intergenic 45.0%
CAATAAGGTTGCTGAAAGCC+TGG + chr6_2:71879584-71879603 None:intergenic 45.0%
TTTAACGGTCCCTTCTGAGT+TGG - chr6_2:71877740-71877759 Msa0927520:intron 45.0%
!! CTGAGTTGGAATCGGTTTTG+AGG - chr6_2:71877754-71877773 Msa0927520:intron 45.0%
ACTTTGTTACAAGGAGGCCA+TGG - chr6_2:71877793-71877812 Msa0927520:intron 45.0%
! GTGATACCTGTTGTGCCATT+TGG - chr6_2:71877935-71877954 Msa0927520:intron 45.0%
CTGGTAAGCAACAGCGAAAA+AGG + chr6_2:71877994-71878013 Msa0927510:intron 45.0%
CTACTCCCGCACATTATAAC+TGG + chr6_2:71878100-71878119 Msa0927510:intron 45.0%
!! GGGAGTAGTTGGTTTGTTTG+CGG - chr6_2:71878112-71878131 Msa0927520:intron 45.0%
! CTCATGTGCGATATTTGGTG+TGG - chr6_2:71878158-71878177 Msa0927520:intron 45.0%
CATTCGAGCAAATACCTGCA+AGG + chr6_2:71878463-71878482 None:intergenic 45.0%
CATGCATAGCGCCAAAATAC+AGG + chr6_2:71878549-71878568 None:intergenic 45.0%
TTGGCAGGTAATGTCATTGG+AGG - chr6_2:71878578-71878597 Msa0927520:intron 45.0%
TTACCGTCAAATCGCAACCT+TGG + chr6_2:71878746-71878765 None:intergenic 45.0%
! TACTCTTAGTGTTACTGCCC+AGG - chr6_2:71879089-71879108 Msa0927520:five_prime_UTR 45.0%
!! AAGGGGCATCTCTATATCCT+GGG + chr6_2:71879109-71879128 None:intergenic 45.0%
AGTGAGAGCTAAAGGTGGAA+GGG + chr6_2:71879127-71879146 None:intergenic 45.0%
! CATAGTGGAGTGAGAGCTAA+AGG + chr6_2:71879135-71879154 None:intergenic 45.0%
CTCCACTATGACATGGAATC+AGG - chr6_2:71879145-71879164 Msa0927520:five_prime_UTR 45.0%
GGACTTCAGAATTGGCAGAA+TGG + chr6_2:71879252-71879271 None:intergenic 45.0%
TCTTCTACAGCAGCTAGCAA+AGG + chr6_2:71879549-71879568 None:intergenic 45.0%
CAATAAGGTTGCTGAAAGCC+TGG + chr6_2:71879584-71879603 None:intergenic 45.0%
! GGTCCCTTCTGAGTTGGAAT+CGG - chr6_2:71877746-71877765 Msa0927520:intron 50.0%
GCGTTGTACATACCAAGCCA+TGG + chr6_2:71877813-71877832 Msa0927510:three_prime_UTR 50.0%
TACAACGCTGCTAGCTACCA+TGG - chr6_2:71877825-71877844 Msa0927520:intron 50.0%
GCACTTCCAAATGGCACAAC+AGG + chr6_2:71877944-71877963 Msa0927510:intron 50.0%
ACCAGATGAGCTGCTCTTTG+AGG - chr6_2:71878009-71878028 Msa0927520:intron 50.0%
ACCTCAAAGAGCAGCTCATC+TGG + chr6_2:71878013-71878032 Msa0927510:intron 50.0%
TTGGCGCTATGCATGCTTGA+AGG - chr6_2:71878554-71878573 Msa0927520:intron 50.0%
TGGCGCTATGCATGCTTGAA+GGG - chr6_2:71878555-71878574 Msa0927520:intron 50.0%
GCTATGCATGCTTGAAGGGT+TGG - chr6_2:71878559-71878578 Msa0927520:intron 50.0%
! GGGTTGGCAGGTAATGTCAT+TGG - chr6_2:71878575-71878594 Msa0927520:intron 50.0%
CAAAATACGACGGCTCATGG+CGG - chr6_2:71878702-71878721 Msa0927520:intron 50.0%
GGCGGAAAATGTGGCTCTTA+CGG - chr6_2:71878720-71878739 Msa0927520:intron 50.0%
AAATGTGGCTCTTACGGCCA+AGG - chr6_2:71878726-71878745 Msa0927520:intron 50.0%
!! GAAGGGGCATCTCTATATCC+TGG + chr6_2:71879110-71879129 None:intergenic 50.0%
GTGAGAGCTAAAGGTGGAAG+GGG + chr6_2:71879126-71879145 None:intergenic 50.0%
GAGTGAGAGCTAAAGGTGGA+AGG + chr6_2:71879128-71879147 None:intergenic 50.0%
AGTGGAGTGAGAGCTAAAGG+TGG + chr6_2:71879132-71879151 None:intergenic 50.0%
GCTCTCACTCCACTATGACA+TGG - chr6_2:71879138-71879157 Msa0927520:five_prime_UTR 50.0%
! GGTCCCTTCTGAGTTGGAAT+CGG - chr6_2:71877746-71877765 Msa0927520:intron 50.0%
GCGTTGTACATACCAAGCCA+TGG + chr6_2:71877813-71877832 Msa0927510:three_prime_UTR 50.0%
TACAACGCTGCTAGCTACCA+TGG - chr6_2:71877825-71877844 Msa0927520:intron 50.0%
GCACTTCCAAATGGCACAAC+AGG + chr6_2:71877944-71877963 Msa0927510:intron 50.0%
ACCAGATGAGCTGCTCTTTG+AGG - chr6_2:71878009-71878028 Msa0927520:intron 50.0%
ACCTCAAAGAGCAGCTCATC+TGG + chr6_2:71878013-71878032 Msa0927510:intron 50.0%
TTGGCGCTATGCATGCTTGA+AGG - chr6_2:71878554-71878573 Msa0927520:intron 50.0%
TGGCGCTATGCATGCTTGAA+GGG - chr6_2:71878555-71878574 Msa0927520:intron 50.0%
GCTATGCATGCTTGAAGGGT+TGG - chr6_2:71878559-71878578 Msa0927520:intron 50.0%
! GGGTTGGCAGGTAATGTCAT+TGG - chr6_2:71878575-71878594 Msa0927520:intron 50.0%
CAAAATACGACGGCTCATGG+CGG - chr6_2:71878702-71878721 Msa0927520:intron 50.0%
GGCGGAAAATGTGGCTCTTA+CGG - chr6_2:71878720-71878739 Msa0927520:intron 50.0%
AAATGTGGCTCTTACGGCCA+AGG - chr6_2:71878726-71878745 Msa0927520:intron 50.0%
!! GAAGGGGCATCTCTATATCC+TGG + chr6_2:71879110-71879129 None:intergenic 50.0%
GTGAGAGCTAAAGGTGGAAG+GGG + chr6_2:71879126-71879145 None:intergenic 50.0%
GAGTGAGAGCTAAAGGTGGA+AGG + chr6_2:71879128-71879147 None:intergenic 50.0%
AGTGGAGTGAGAGCTAAAGG+TGG + chr6_2:71879132-71879151 None:intergenic 50.0%
GCTCTCACTCCACTATGACA+TGG - chr6_2:71879138-71879157 Msa0927520:five_prime_UTR 50.0%
GTTACAAGGAGGCCATGGCT+TGG - chr6_2:71877798-71877817 Msa0927520:intron 55.0%
! TGCATGCTTGAAGGGTTGGC+AGG - chr6_2:71878563-71878582 Msa0927520:intron 55.0%
! ACGGCTCATGGCGGAAAATG+TGG - chr6_2:71878711-71878730 Msa0927520:intron 55.0%
CGGCCAAGGTTGCGATTTGA+CGG - chr6_2:71878740-71878759 Msa0927520:intron 55.0%
GTTACAAGGAGGCCATGGCT+TGG - chr6_2:71877798-71877817 Msa0927520:intron 55.0%
! TGCATGCTTGAAGGGTTGGC+AGG - chr6_2:71878563-71878582 Msa0927520:intron 55.0%
! ACGGCTCATGGCGGAAAATG+TGG - chr6_2:71878711-71878730 Msa0927520:intron 55.0%
CGGCCAAGGTTGCGATTTGA+CGG - chr6_2:71878740-71878759 Msa0927520:intron 55.0%
AGAAGAACCTGCTCTGCGCC+AGG - chr6_2:71879563-71879582 Msa0927520:five_prime_UTR 60.0%
AGAAGAACCTGCTCTGCGCC+AGG - chr6_2:71879563-71879582 Msa0927520:five_prime_UTR 60.0%
CTGAAAGCCTGGCGCAGAGC+AGG + chr6_2:71879573-71879592 None:intergenic 65.0%
CTGAAAGCCTGGCGCAGAGC+AGG + chr6_2:71879573-71879592 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 71877297 71879605 71877297 ID=Msa0927520;Name=Msa0927520
chr6_2 mRNA 71877297 71879605 71877297 ID=Msa0927520-mRNA-1;Parent=Msa0927520;Name=Msa0927520-mRNA-1;_AED=0.27;_eAED=0.27;_QI=734|0.66|0.75|0.75|0.33|0.5|4|0|59
chr6_2 exon 71877297 71877454 71877297 ID=Msa0927520-mRNA-1:exon:12081;Parent=Msa0927520-mRNA-1
chr6_2 exon 71878318 71878434 71878318 ID=Msa0927520-mRNA-1:exon:12080;Parent=Msa0927520-mRNA-1
chr6_2 exon 71878872 71878984 71878872 ID=Msa0927520-mRNA-1:exon:12079;Parent=Msa0927520-mRNA-1
chr6_2 exon 71879083 71879605 71879083 ID=Msa0927520-mRNA-1:exon:12078;Parent=Msa0927520-mRNA-1
chr6_2 five_prime_UTR 71879083 71879605 71879083 ID=Msa0927520-mRNA-1:five_prime_utr;Parent=Msa0927520-mRNA-1
chr6_2 five_prime_UTR 71878872 71878984 71878872 ID=Msa0927520-mRNA-1:five_prime_utr;Parent=Msa0927520-mRNA-1
chr6_2 five_prime_UTR 71878337 71878434 71878337 ID=Msa0927520-mRNA-1:five_prime_utr;Parent=Msa0927520-mRNA-1
chr6_2 CDS 71878318 71878336 71878318 ID=Msa0927520-mRNA-1:cds;Parent=Msa0927520-mRNA-1
chr6_2 CDS 71877297 71877454 71877297 ID=Msa0927520-mRNA-1:cds;Parent=Msa0927520-mRNA-1
Gene Sequence

>Msa0927520

ATGCTTGAAGGGTTGGCAGACTGCAATTTTTCCAAAGTATTGGCAGACTTGGTTGATACAATCAAACCTCTTGCTCAGAATCAAAAACGTGTAATAGAACATAGTCTGCTCTCATCACCTTTGCTAGCTGCTGTAGAAGAACCTGCTCTGCGCCAGGCTTTCAGCAACCTTATTGAA
Protein sequence

>Msa0927520

MLEGLADCNFSKVLADLVDTIKPLAQNQKRVIEHSLLSSPLLAAVEEPALRQAFSNLIE