Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0928600 | XP_013467661.1 | 96.324 | 136 | 5 | 0 | 4 | 139 | 121 | 256 | 4.44e-85 | 263 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0735170 | Msa0928600 | 0.803842 | 2.874225e-49 | -8.615850e-47 |
| Msa0216910 | Msa0928600 | 0.801557 | 8.512630e-49 | -8.615850e-47 |
| Msa0849030 | Msa0928600 | 0.806630 | 7.494334e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0928600 | MtrunA17_Chr1g0175161 | 96.324 | 136 | 5 | 0 | 4 | 139 | 121 | 256 | 4.08e-89 | 263 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0928600 | AT3G57990.1 | 50.000 | 102 | 38 | 3 | 25 | 113 | 134 | 235 | 1.44e-23 | 94.7 |
Find 48 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCGGGAAGGTGCCGTCGGC+TGG | 0.220747 | 6_2:+74450022 | Msa0928600:CDS |
| CCGTCGGCTGGGGTGATTGC+AGG | 0.226839 | 6_2:+74450034 | Msa0928600:CDS |
| TTTCTTGTGATGGATAAAAT+TGG | 0.227367 | 6_2:+74450187 | Msa0928600:CDS |
| TCCGCAAAACGGTGGCGTTT+TGG | 0.243707 | 6_2:+74449943 | Msa0928600:CDS |
| CTGAGGTTGCGGCGAGGACT+AGG | 0.264777 | 6_2:+74450071 | Msa0928600:CDS |
| TAGGGTTCCGGCTGAGGTTA+AGG | 0.286798 | 6_2:+74450138 | Msa0928600:CDS |
| GTTAAGGGCGAAAGCGCGTT+TGG | 0.350617 | 6_2:+74450154 | Msa0928600:CDS |
| TCGGGAAGGTGCCGTCGGCT+GGG | 0.364504 | 6_2:+74450023 | Msa0928600:CDS |
| AAAGGAAAGGTTGGTGCTGC+TGG | 0.377756 | 6_2:+74450256 | Msa0928600:CDS |
| GAGGACTAGGGTTCCGATAA+GGG | 0.381559 | 6_2:+74450084 | Msa0928600:CDS |
| ACCGCGGCACGTCCCCTTAT+CGG | 0.382309 | 6_2:-74450097 | None:intergenic |
| CGGGAAGGTGCCGTCGGCTG+GGG | 0.385505 | 6_2:+74450024 | Msa0928600:CDS |
| TTCTTGTGATGGATAAAATT+GGG | 0.414253 | 6_2:+74450188 | Msa0928600:CDS |
| CGAGGACTAGGGTTCCGATA+AGG | 0.427189 | 6_2:+74450083 | Msa0928600:CDS |
| TTTGTTGAATCTCCGGTGAT+TGG | 0.435435 | 6_2:+74449989 | Msa0928600:CDS |
| CAACATGTCGAAATCAAATC+AGG | 0.441154 | 6_2:+74449590 | None:intergenic |
| ATTTGAGTTTGTTGAATCTC+CGG | 0.443909 | 6_2:+74449982 | Msa0928600:CDS |
| AGGGTTCCGGCTGAGGTTAA+GGG | 0.446628 | 6_2:+74450139 | Msa0928600:CDS |
| TTAAGGGCGAAAGCGCGTTT+GGG | 0.463653 | 6_2:+74450155 | Msa0928600:CDS |
| CTTTCGCCCTTAACCTCAGC+CGG | 0.466668 | 6_2:-74450145 | None:intergenic |
| GAGGGTTCCGTTTCTTGTGA+TGG | 0.480532 | 6_2:+74450177 | Msa0928600:CDS |
| GTGATTGGTGGGTTTAGTGT+CGG | 0.483284 | 6_2:+74450004 | Msa0928600:CDS |
| GATTTGAAGAATTTGAAGAA+AGG | 0.489031 | 6_2:+74450238 | Msa0928600:CDS |
| CTCGGCAACACAGCACTTGT+CGG | 0.490850 | 6_2:-74450289 | None:intergenic |
| TGATTGGTGGGTTTAGTGTC+GGG | 0.491545 | 6_2:+74450005 | Msa0928600:CDS |
| TTCAAATCCCCACACTCCGC+TGG | 0.499435 | 6_2:-74450223 | None:intergenic |
| AACATTTGCCAGCGGAGTGT+GGG | 0.501336 | 6_2:+74450215 | Msa0928600:CDS |
| GTTGAATCTCCGGTGATTGG+TGG | 0.516218 | 6_2:+74449992 | Msa0928600:CDS |
| TGAGGTTGCGGCGAGGACTA+GGG | 0.516942 | 6_2:+74450072 | Msa0928600:CDS |
| CGTCGGCTGGGGTGATTGCA+GGG | 0.520998 | 6_2:+74450035 | Msa0928600:CDS |
| GAACATTTGCCAGCGGAGTG+TGG | 0.526006 | 6_2:+74450214 | Msa0928600:CDS |
| TCCGATAAGGGGACGTGCCG+CGG | 0.528280 | 6_2:+74450096 | Msa0928600:CDS |
| GAAGAATTTGAAGAAAGGAA+AGG | 0.533083 | 6_2:+74450243 | Msa0928600:CDS |
| AATTTGAAGAAAGGAAAGGT+TGG | 0.544445 | 6_2:+74450247 | Msa0928600:CDS |
| AGGGCGAAAGCGCGTTTGGG+AGG | 0.549372 | 6_2:+74450158 | Msa0928600:CDS |
| TTGAATCTCCGGTGATTGGT+GGG | 0.555286 | 6_2:+74449993 | Msa0928600:CDS |
| GGGCGAAAGCGCGTTTGGGA+GGG | 0.557564 | 6_2:+74450159 | Msa0928600:CDS |
| CCTGCAATCACCCCAGCCGA+CGG | 0.569702 | 6_2:-74450034 | None:intergenic |
| TAGTGTCGGGAAGGTGCCGT+CGG | 0.576506 | 6_2:+74450018 | Msa0928600:CDS |
| ACACTAAACCCACCAATCAC+CGG | 0.580138 | 6_2:-74450001 | None:intergenic |
| TGGTGGGTTTAGTGTCGGGA+AGG | 0.594609 | 6_2:+74450009 | Msa0928600:CDS |
| AGGACTAGGGTTCCGATAAG+GGG | 0.601572 | 6_2:+74450085 | Msa0928600:CDS |
| ACATTTGCCAGCGGAGTGTG+GGG | 0.619702 | 6_2:+74450216 | Msa0928600:CDS |
| CTTCACTGCAAAACTCGTCT+CGG | 0.621132 | 6_2:-74450307 | None:intergenic |
| TCTTGTGATGGATAAAATTG+GGG | 0.621136 | 6_2:+74450189 | Msa0928600:CDS |
| TGTGATGGATAAAATTGGGG+TGG | 0.626777 | 6_2:+74450192 | Msa0928600:CDS |
| CGGGGACTGAGGTTGCGGCG+AGG | 0.653848 | 6_2:+74450065 | Msa0928600:CDS |
| TGGGGTGGAACATTTGCCAG+CGG | 0.659065 | 6_2:+74450207 | Msa0928600:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGAAAATTTGATTTCAATT+TGG | + | chr6_2:74449715-74449734 | Msa0928600:intron | 15.0% |
| !!! | AGAAAATTTGATTTCAATTT+GGG | + | chr6_2:74449716-74449735 | Msa0928600:intron | 15.0% |
| !!! | GTGTTTTTGTTTGTTTAAAA+TGG | + | chr6_2:74449655-74449674 | Msa0928600:intron | 20.0% |
| !! | TCTCAATTTGAAAATTGTAT+TGG | + | chr6_2:74449791-74449810 | Msa0928600:intron | 20.0% |
| !!! | TGGTTTTGTTTAGATCAATA+AGG | + | chr6_2:74449675-74449694 | Msa0928600:intron | 25.0% |
| ! | CACATTAATCAGTCAATTTA+GGG | - | chr6_2:74449759-74449778 | None:intergenic | 25.0% |
| !! | ACAAAAACCATTTTAAACCT+AGG | + | chr6_2:74449858-74449877 | Msa0928600:intron | 25.0% |
| !! | AGTTCGATTTTAGATGTTAA+GGG | + | chr6_2:74449911-74449930 | Msa0928600:CDS | 25.0% |
| !! | ATTTTATCCATCACAAGAAA+CGG | - | chr6_2:74450187-74450206 | None:intergenic | 25.0% |
| !!! | TTTCTTGTGATGGATAAAAT+TGG | + | chr6_2:74450187-74450206 | Msa0928600:CDS | 25.0% |
| !!! | TTCTTGTGATGGATAAAATT+GGG | + | chr6_2:74450188-74450207 | Msa0928600:CDS | 25.0% |
| ! | GATTTGAAGAATTTGAAGAA+AGG | + | chr6_2:74450238-74450257 | Msa0928600:CDS | 25.0% |
| AATAAGGTTTCTCTGTTAGT+TGG | + | chr6_2:74449691-74449710 | Msa0928600:intron | 30.0% | |
| GCACATTAATCAGTCAATTT+AGG | - | chr6_2:74449760-74449779 | None:intergenic | 30.0% | |
| ! | AACCATTTTAAACCTAGGTT+TGG | + | chr6_2:74449863-74449882 | Msa0928600:intron | 30.0% |
| ! | ACCATTTTAAACCTAGGTTT+GGG | + | chr6_2:74449864-74449883 | Msa0928600:intron | 30.0% |
| ! | GAGTTCGATTTTAGATGTTA+AGG | + | chr6_2:74449910-74449929 | Msa0928600:CDS | 30.0% |
| !! | ATTTGAGTTTGTTGAATCTC+CGG | + | chr6_2:74449982-74450001 | Msa0928600:CDS | 30.0% |
| !! | TCTTGTGATGGATAAAATTG+GGG | + | chr6_2:74450189-74450208 | Msa0928600:CDS | 30.0% |
| GAAGAATTTGAAGAAAGGAA+AGG | + | chr6_2:74450243-74450262 | Msa0928600:CDS | 30.0% | |
| AATTTGAAGAAAGGAAAGGT+TGG | + | chr6_2:74450247-74450266 | Msa0928600:CDS | 30.0% | |
| ! | AATCAGTCAATTTAGGGCAT+CGG | - | chr6_2:74449753-74449772 | None:intergenic | 35.0% |
| ! | CCATTTTAAACCTAGGTTTG+GGG | + | chr6_2:74449865-74449884 | Msa0928600:intron | 35.0% |
| AAAGAAAAATCCCCAAACCT+AGG | - | chr6_2:74449878-74449897 | None:intergenic | 35.0% | |
| CCCCAAACCTAGGTTTAAAA+TGG | - | chr6_2:74449868-74449887 | None:intergenic | 40.0% | |
| ! | GGTTATGCTTTTCCGCAAAA+CGG | + | chr6_2:74449932-74449951 | Msa0928600:CDS | 40.0% |
| TTTGTTGAATCTCCGGTGAT+TGG | + | chr6_2:74449989-74450008 | Msa0928600:CDS | 40.0% | |
| !! | TGTGATGGATAAAATTGGGG+TGG | + | chr6_2:74450192-74450211 | Msa0928600:CDS | 40.0% |
| ! | TATGCTTTTCCGCAAAACGG+TGG | + | chr6_2:74449935-74449954 | Msa0928600:CDS | 45.0% |
| TTGAATCTCCGGTGATTGGT+GGG | + | chr6_2:74449993-74450012 | Msa0928600:CDS | 45.0% | |
| ACACTAAACCCACCAATCAC+CGG | - | chr6_2:74450004-74450023 | None:intergenic | 45.0% | |
| !! | GTGATTGGTGGGTTTAGTGT+CGG | + | chr6_2:74450004-74450023 | Msa0928600:CDS | 45.0% |
| !! | TGATTGGTGGGTTTAGTGTC+GGG | + | chr6_2:74450005-74450024 | Msa0928600:CDS | 45.0% |
| !! | GGTGATTGCAGGGTTGTTTT+CGG | + | chr6_2:74450045-74450064 | Msa0928600:CDS | 45.0% |
| !! | GTGATTGCAGGGTTGTTTTC+GGG | + | chr6_2:74450046-74450065 | Msa0928600:CDS | 45.0% |
| !! | TGATTGCAGGGTTGTTTTCG+GGG | + | chr6_2:74450047-74450066 | Msa0928600:CDS | 45.0% |
| !! | TGAATTTTAGGTGGGGGGTT+AGG | + | chr6_2:74450119-74450138 | Msa0928600:CDS | 45.0% |
| !! | GAATTTTAGGTGGGGGGTTA+GGG | + | chr6_2:74450120-74450139 | Msa0928600:CDS | 45.0% |
| !!! | TTTGTATAATTTTTAATTTT+TGG | - | chr6_2:74449843-74449862 | None:intergenic | 5.0% |
| GTTGAATCTCCGGTGATTGG+TGG | + | chr6_2:74449992-74450011 | Msa0928600:CDS | 50.0% | |
| GAGGACTAGGGTTCCGATAA+GGG | + | chr6_2:74450084-74450103 | Msa0928600:CDS | 50.0% | |
| AGGACTAGGGTTCCGATAAG+GGG | + | chr6_2:74450085-74450104 | Msa0928600:CDS | 50.0% | |
| !! | TGCCGCGGTGAATTTTAGGT+GGG | + | chr6_2:74450111-74450130 | Msa0928600:CDS | 50.0% |
| ! | TTAAGGGCGAAAGCGCGTTT+GGG | + | chr6_2:74450155-74450174 | Msa0928600:CDS | 50.0% |
| GAGGGTTCCGTTTCTTGTGA+TGG | + | chr6_2:74450177-74450196 | Msa0928600:CDS | 50.0% | |
| AACATTTGCCAGCGGAGTGT+GGG | + | chr6_2:74450215-74450234 | Msa0928600:CDS | 50.0% | |
| !! | AAAGGAAAGGTTGGTGCTGC+TGG | + | chr6_2:74450256-74450275 | Msa0928600:CDS | 50.0% |
| ! | TCCGCAAAACGGTGGCGTTT+TGG | + | chr6_2:74449943-74449962 | Msa0928600:CDS | 55.0% |
| ! | CCCAAAACGCCACCGTTTTG+CGG | - | chr6_2:74449947-74449966 | None:intergenic | 55.0% |
| ! | CCGCAAAACGGTGGCGTTTT+GGG | + | chr6_2:74449944-74449963 | Msa0928600:CDS | 55.0% |
| ! | CGCAAAACGGTGGCGTTTTG+GGG | + | chr6_2:74449945-74449964 | Msa0928600:CDS | 55.0% |
| !! | TGGTGGGTTTAGTGTCGGGA+AGG | + | chr6_2:74450009-74450028 | Msa0928600:CDS | 55.0% |
| !! | AGGGTTGTTTTCGGGGACTG+AGG | + | chr6_2:74450054-74450073 | Msa0928600:CDS | 55.0% |
| !! | GTTTTCGGGGACTGAGGTTG+CGG | + | chr6_2:74450060-74450079 | Msa0928600:CDS | 55.0% |
| CGAGGACTAGGGTTCCGATA+AGG | + | chr6_2:74450083-74450102 | Msa0928600:CDS | 55.0% | |
| ! | GACGTGCCGCGGTGAATTTT+AGG | + | chr6_2:74450107-74450126 | Msa0928600:CDS | 55.0% |
| ! | GTGCCGCGGTGAATTTTAGG+TGG | + | chr6_2:74450110-74450129 | Msa0928600:CDS | 55.0% |
| !! | GCCGCGGTGAATTTTAGGTG+GGG | + | chr6_2:74450112-74450131 | Msa0928600:CDS | 55.0% |
| CCCCCACCTAAAATTCACCG+CGG | - | chr6_2:74450116-74450135 | None:intergenic | 55.0% | |
| !! | CCGCGGTGAATTTTAGGTGG+GGG | + | chr6_2:74450113-74450132 | Msa0928600:CDS | 55.0% |
| !! | CGCGGTGAATTTTAGGTGGG+GGG | + | chr6_2:74450114-74450133 | Msa0928600:CDS | 55.0% |
| TAGGGTTCCGGCTGAGGTTA+AGG | + | chr6_2:74450138-74450157 | Msa0928600:CDS | 55.0% | |
| AGGGTTCCGGCTGAGGTTAA+GGG | + | chr6_2:74450139-74450158 | Msa0928600:CDS | 55.0% | |
| CTTTCGCCCTTAACCTCAGC+CGG | - | chr6_2:74450148-74450167 | None:intergenic | 55.0% | |
| ! | GTTAAGGGCGAAAGCGCGTT+TGG | + | chr6_2:74450154-74450173 | Msa0928600:CDS | 55.0% |
| TGGGGTGGAACATTTGCCAG+CGG | + | chr6_2:74450207-74450226 | Msa0928600:CDS | 55.0% | |
| GAACATTTGCCAGCGGAGTG+TGG | + | chr6_2:74450214-74450233 | Msa0928600:CDS | 55.0% | |
| ACATTTGCCAGCGGAGTGTG+GGG | + | chr6_2:74450216-74450235 | Msa0928600:CDS | 55.0% | |
| TTCAAATCCCCACACTCCGC+TGG | - | chr6_2:74450226-74450245 | None:intergenic | 55.0% | |
| CTCGGCAACACAGCACTTGT+CGG | - | chr6_2:74450292-74450311 | None:intergenic | 55.0% | |
| !! | TAGTGTCGGGAAGGTGCCGT+CGG | + | chr6_2:74450018-74450037 | Msa0928600:CDS | 60.0% |
| TGAGGTTGCGGCGAGGACTA+GGG | + | chr6_2:74450072-74450091 | Msa0928600:CDS | 60.0% | |
| TAGGTGGGGGGTTAGGGTTC+CGG | + | chr6_2:74450126-74450145 | Msa0928600:CDS | 60.0% | |
| CCTGCAATCACCCCAGCCGA+CGG | - | chr6_2:74450037-74450056 | None:intergenic | 65.0% | |
| ! | CGTCGGCTGGGGTGATTGCA+GGG | + | chr6_2:74450035-74450054 | Msa0928600:CDS | 65.0% |
| CTGAGGTTGCGGCGAGGACT+AGG | + | chr6_2:74450071-74450090 | Msa0928600:CDS | 65.0% | |
| TCCGATAAGGGGACGTGCCG+CGG | + | chr6_2:74450096-74450115 | Msa0928600:CDS | 65.0% | |
| ACCGCGGCACGTCCCCTTAT+CGG | - | chr6_2:74450100-74450119 | None:intergenic | 65.0% | |
| ! | AGGGCGAAAGCGCGTTTGGG+AGG | + | chr6_2:74450158-74450177 | Msa0928600:CDS | 65.0% |
| ! | GGGCGAAAGCGCGTTTGGGA+GGG | + | chr6_2:74450159-74450178 | Msa0928600:CDS | 65.0% |
| !! | TCGGGAAGGTGCCGTCGGCT+GGG | + | chr6_2:74450023-74450042 | Msa0928600:CDS | 70.0% |
| ! | CCGTCGGCTGGGGTGATTGC+AGG | + | chr6_2:74450034-74450053 | Msa0928600:CDS | 70.0% |
| GGGGGTTAGGGTTCCGGCTG+AGG | + | chr6_2:74450132-74450151 | Msa0928600:CDS | 70.0% | |
| !! | GTCGGGAAGGTGCCGTCGGC+TGG | + | chr6_2:74450022-74450041 | Msa0928600:CDS | 75.0% |
| !! | CGGGAAGGTGCCGTCGGCTG+GGG | + | chr6_2:74450024-74450043 | Msa0928600:CDS | 75.0% |
| CGGGGACTGAGGTTGCGGCG+AGG | + | chr6_2:74450065-74450084 | Msa0928600:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 74449594 | 74450327 | 74449594 | ID=Msa0928600;Name=Msa0928600 |
| chr6_2 | mRNA | 74449594 | 74450327 | 74449594 | ID=Msa0928600-mRNA-1;Parent=Msa0928600;Name=Msa0928600-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0|0|0.5|1|1|2|0|147 |
| chr6_2 | exon | 74449594 | 74449611 | 74449594 | ID=Msa0928600-mRNA-1:exon:12666;Parent=Msa0928600-mRNA-1 |
| chr6_2 | exon | 74449902 | 74450327 | 74449902 | ID=Msa0928600-mRNA-1:exon:12667;Parent=Msa0928600-mRNA-1 |
| chr6_2 | CDS | 74449594 | 74449611 | 74449594 | ID=Msa0928600-mRNA-1:cds;Parent=Msa0928600-mRNA-1 |
| chr6_2 | CDS | 74449902 | 74450327 | 74449902 | ID=Msa0928600-mRNA-1:cds;Parent=Msa0928600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
