Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0931500 | XP_003605353.1 | 94.472 | 199 | 11 | 0 | 1 | 199 | 1 | 199 | 3.11e-136 | 389 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0931500 | sp|Q9LZX1|LOR15_ARATH | 53.769 | 199 | 84 | 4 | 6 | 197 | 16 | 213 | 2.44e-71 | 218 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0672680 | Msa0931500 | 0.868214 | 7.712484e-66 | -8.615850e-47 |
| Msa0585240 | Msa0931500 | 0.800047 | 1.730569e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0931500 | MtrunA17_Chr4g0013291 | 94.472 | 199 | 11 | 0 | 1 | 199 | 1 | 199 | 2.86e-140 | 389 |
| Msa0931500 | MtrunA17_Chr7g0244371 | 61.053 | 190 | 69 | 2 | 12 | 197 | 19 | 207 | 1.18e-81 | 241 |
| Msa0931500 | MtrunA17_Chr7g0244321 | 60.733 | 191 | 69 | 3 | 13 | 198 | 21 | 210 | 1.02e-80 | 239 |
| Msa0931500 | MtrunA17_Chr7g0244331 | 60.938 | 192 | 69 | 3 | 11 | 197 | 19 | 209 | 1.54e-80 | 239 |
| Msa0931500 | MtrunA17_Chr7g0244341 | 59.259 | 189 | 71 | 3 | 15 | 198 | 23 | 210 | 1.12e-77 | 232 |
| Msa0931500 | MtrunA17_Chr7g0244361 | 53.769 | 199 | 80 | 3 | 12 | 200 | 44 | 240 | 1.03e-70 | 215 |
| Msa0931500 | MtrunA17_Chr7g0244111 | 64.407 | 118 | 42 | 0 | 80 | 197 | 4 | 121 | 1.69e-55 | 172 |
| Msa0931500 | MtrunA17_Chr1g0150081 | 42.857 | 189 | 93 | 6 | 13 | 192 | 3 | 185 | 4.01e-39 | 132 |
| Msa0931500 | MtrunA17_Chr2g0333101 | 32.164 | 171 | 99 | 7 | 39 | 199 | 43 | 206 | 1.72e-20 | 85.5 |
| Msa0931500 | MtrunA17_Chr2g0333111 | 30.178 | 169 | 105 | 6 | 39 | 199 | 44 | 207 | 8.03e-17 | 75.9 |
| Msa0931500 | MtrunA17_Chr4g0003271 | 25.888 | 197 | 132 | 7 | 10 | 197 | 2 | 193 | 2.18e-16 | 74.3 |
| Msa0931500 | MtrunA17_Chr1g0150101 | 43.038 | 79 | 41 | 2 | 114 | 192 | 5 | 79 | 3.39e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0931500 | AT5G01750.2 | 53.769 | 199 | 84 | 4 | 6 | 197 | 16 | 213 | 2.48e-72 | 218 |
| Msa0931500 | AT3G11740.1 | 49.223 | 193 | 91 | 4 | 14 | 200 | 2 | 193 | 1.15e-61 | 191 |
| Msa0931500 | AT1G33840.1 | 49.189 | 185 | 88 | 3 | 15 | 194 | 27 | 210 | 1.02e-55 | 176 |
| Msa0931500 | AT2G14560.1 | 49.198 | 187 | 89 | 3 | 15 | 196 | 7 | 192 | 1.70e-55 | 175 |
| Msa0931500 | AT2G14560.3 | 49.198 | 187 | 89 | 3 | 15 | 196 | 67 | 252 | 1.80e-54 | 174 |
| Msa0931500 | AT5G01750.1 | 51.899 | 158 | 68 | 4 | 6 | 156 | 16 | 172 | 1.38e-50 | 162 |
| Msa0931500 | AT2G14560.2 | 50.676 | 148 | 67 | 3 | 15 | 157 | 7 | 153 | 1.07e-42 | 142 |
| Msa0931500 | AT2G14560.4 | 50.676 | 148 | 67 | 3 | 15 | 157 | 67 | 213 | 1.26e-41 | 141 |
| Msa0931500 | AT1G33840.2 | 47.297 | 148 | 72 | 3 | 15 | 157 | 27 | 173 | 1.26e-38 | 131 |
| Msa0931500 | AT3G16900.1 | 33.000 | 200 | 109 | 6 | 1 | 195 | 1 | 180 | 2.09e-32 | 115 |
| Msa0931500 | AT3G56180.2 | 26.131 | 199 | 138 | 4 | 2 | 193 | 5 | 201 | 3.61e-20 | 84.7 |
| Msa0931500 | AT3G15810.1 | 30.303 | 165 | 104 | 4 | 39 | 197 | 45 | 204 | 2.38e-19 | 82.8 |
| Msa0931500 | AT3G56180.1 | 25.628 | 199 | 137 | 5 | 2 | 193 | 5 | 199 | 6.92e-18 | 78.6 |
| Msa0931500 | AT1G80120.1 | 29.762 | 168 | 104 | 5 | 39 | 197 | 31 | 193 | 7.70e-14 | 67.8 |
Find 26 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAAGATAATCTCACGGTT+AGG | 0.384584 | 6_2:+81018352 | Msa0931500:CDS |
| GTGTTCAAAGTGTTGCGTTT+GGG | 0.407302 | 6_2:+81018331 | Msa0931500:CDS |
| CTTCTTTAGTATTACTAGCT+AGG | 0.413877 | 6_2:-81018070 | None:intergenic |
| CTGTTGACCTATCAATTGTA+AGG | 0.415763 | 6_2:+81017653 | Msa0931500:CDS |
| AGTGTTCAAAGTGTTGCGTT+TGG | 0.433678 | 6_2:+81018330 | Msa0931500:CDS |
| TGACATTCCTTACAATTGAT+AGG | 0.450507 | 6_2:-81017660 | None:intergenic |
| GTAAGAGACTGTGATCATAA+AGG | 0.456930 | 6_2:-81017747 | None:intergenic |
| TGTGATCATAAAGGAATTTG+AGG | 0.464343 | 6_2:-81017738 | None:intergenic |
| CGATCTTGTGTTGTTTATGC+TGG | 0.470752 | 6_2:+81018131 | Msa0931500:CDS |
| GAAAGATAATCTCACGGTTA+GGG | 0.472008 | 6_2:+81018353 | Msa0931500:CDS |
| TTGATTGTAGATGCATAAGA+AGG | 0.480843 | 6_2:+81018305 | Msa0931500:intron |
| ATGAATGCATAGTCAATGTT+GGG | 0.488228 | 6_2:-81018381 | None:intergenic |
| TACTTTCACCTCGAAAAGCT+TGG | 0.493576 | 6_2:-81017974 | None:intergenic |
| ATCTCATCACATTGTTGCCC+AGG | 0.500173 | 6_2:+81018157 | Msa0931500:CDS |
| GATGAATGCATAGTCAATGT+TGG | 0.514312 | 6_2:-81018382 | None:intergenic |
| AATTATGAGAGTGGTATCAT+TGG | 0.521876 | 6_2:+81017607 | Msa0931500:CDS |
| TTTCTTATACAGGCAATGAC+GGG | 0.543853 | 6_2:+81017939 | Msa0931500:intron |
| AGTCTCTTACTTGATGCAGC+TGG | 0.550594 | 6_2:+81017760 | Msa0931500:CDS |
| TGTCATGACGCTGCGACGAA+AGG | 0.555705 | 6_2:+81017792 | Msa0931500:CDS |
| ATTGATAGGTCAACAGAATG+AGG | 0.558385 | 6_2:-81017646 | None:intergenic |
| ACTCTCATAATTTGCTGCAG+AGG | 0.575251 | 6_2:-81017596 | None:intergenic |
| GAATGAGGAACACGATATTG+AGG | 0.585084 | 6_2:-81017631 | None:intergenic |
| TCTGCAGCAAATTATGAGAG+TGG | 0.622628 | 6_2:+81017598 | Msa0931500:CDS |
| TTTGATCTTTGTTTGAAACA+TGG | 0.637933 | 6_2:-81018036 | None:intergenic |
| GTTTGGGAAAGATAATCTCA+CGG | 0.663482 | 6_2:+81018347 | Msa0931500:CDS |
| CATTGACTATGCATTCATCG+TGG | 0.756924 | 6_2:+81018386 | Msa0931500:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAATTGCATTATTAA+TGG | - | chr6_2:81018237-81018256 | None:intergenic | 10.0% |
| !! | TAAAAAAATTGCATTATTAA+TGG | - | chr6_2:81018237-81018256 | None:intergenic | 10.0% |
| !! | ACGGTAAAATTTTCTTATAC+AGG | + | chr6_2:81017929-81017948 | Msa0931500:intron | 25.0% |
| !! | TTTGATCTTTGTTTGAAACA+TGG | - | chr6_2:81018039-81018058 | None:intergenic | 25.0% |
| !! | AGAAGATACTAGTGATTTTA+AGG | + | chr6_2:81018088-81018107 | Msa0931500:CDS | 25.0% |
| !! | TAAACTTGCTTCTATAGAAA+GGG | - | chr6_2:81018189-81018208 | None:intergenic | 25.0% |
| !! | ATAAACTTGCTTCTATAGAA+AGG | - | chr6_2:81018190-81018209 | None:intergenic | 25.0% |
| !! | ACGGTAAAATTTTCTTATAC+AGG | + | chr6_2:81017929-81017948 | Msa0931500:intron | 25.0% |
| !! | TTTGATCTTTGTTTGAAACA+TGG | - | chr6_2:81018039-81018058 | None:intergenic | 25.0% |
| !! | AGAAGATACTAGTGATTTTA+AGG | + | chr6_2:81018088-81018107 | Msa0931500:CDS | 25.0% |
| !! | TAAACTTGCTTCTATAGAAA+GGG | - | chr6_2:81018189-81018208 | None:intergenic | 25.0% |
| !! | ATAAACTTGCTTCTATAGAA+AGG | - | chr6_2:81018190-81018209 | None:intergenic | 25.0% |
| AATTATGAGAGTGGTATCAT+TGG | + | chr6_2:81017607-81017626 | Msa0931500:CDS | 30.0% | |
| TGACATTCCTTACAATTGAT+AGG | - | chr6_2:81017663-81017682 | None:intergenic | 30.0% | |
| ! | TTCACAGTCACAGATTTTAA+TGG | + | chr6_2:81017700-81017719 | Msa0931500:CDS | 30.0% |
| TGTGATCATAAAGGAATTTG+AGG | - | chr6_2:81017741-81017760 | None:intergenic | 30.0% | |
| CAAAAATGATCAAAATACGC+AGG | - | chr6_2:81017843-81017862 | None:intergenic | 30.0% | |
| ! | TTTTCTTATACAGGCAATGA+CGG | + | chr6_2:81017938-81017957 | Msa0931500:intron | 30.0% |
| CTTCTTTAGTATTACTAGCT+AGG | - | chr6_2:81018073-81018092 | None:intergenic | 30.0% | |
| !!! | ACTAGTGATTTTAAGGTCAA+AGG | + | chr6_2:81018095-81018114 | Msa0931500:CDS | 30.0% |
| !!! | ATTTTAAGGTCAAAGGAAGT+TGG | + | chr6_2:81018102-81018121 | Msa0931500:CDS | 30.0% |
| TTGATTGTAGATGCATAAGA+AGG | + | chr6_2:81018305-81018324 | Msa0931500:intron | 30.0% | |
| ATGAATGCATAGTCAATGTT+GGG | - | chr6_2:81018384-81018403 | None:intergenic | 30.0% | |
| AATTATGAGAGTGGTATCAT+TGG | + | chr6_2:81017607-81017626 | Msa0931500:CDS | 30.0% | |
| TGACATTCCTTACAATTGAT+AGG | - | chr6_2:81017663-81017682 | None:intergenic | 30.0% | |
| ! | TTCACAGTCACAGATTTTAA+TGG | + | chr6_2:81017700-81017719 | Msa0931500:CDS | 30.0% |
| TGTGATCATAAAGGAATTTG+AGG | - | chr6_2:81017741-81017760 | None:intergenic | 30.0% | |
| CAAAAATGATCAAAATACGC+AGG | - | chr6_2:81017843-81017862 | None:intergenic | 30.0% | |
| ! | TTTTCTTATACAGGCAATGA+CGG | + | chr6_2:81017938-81017957 | Msa0931500:intron | 30.0% |
| CTTCTTTAGTATTACTAGCT+AGG | - | chr6_2:81018073-81018092 | None:intergenic | 30.0% | |
| !!! | ACTAGTGATTTTAAGGTCAA+AGG | + | chr6_2:81018095-81018114 | Msa0931500:CDS | 30.0% |
| !!! | ATTTTAAGGTCAAAGGAAGT+TGG | + | chr6_2:81018102-81018121 | Msa0931500:CDS | 30.0% |
| TTGATTGTAGATGCATAAGA+AGG | + | chr6_2:81018305-81018324 | Msa0931500:intron | 30.0% | |
| ATGAATGCATAGTCAATGTT+GGG | - | chr6_2:81018384-81018403 | None:intergenic | 30.0% | |
| ATTGATAGGTCAACAGAATG+AGG | - | chr6_2:81017649-81017668 | None:intergenic | 35.0% | |
| CTGTTGACCTATCAATTGTA+AGG | + | chr6_2:81017653-81017672 | Msa0931500:CDS | 35.0% | |
| GTAAGAGACTGTGATCATAA+AGG | - | chr6_2:81017750-81017769 | None:intergenic | 35.0% | |
| TTTCTTATACAGGCAATGAC+GGG | + | chr6_2:81017939-81017958 | Msa0931500:intron | 35.0% | |
| GTTTGGGAAAGATAATCTCA+CGG | + | chr6_2:81018347-81018366 | Msa0931500:CDS | 35.0% | |
| GAAAGATAATCTCACGGTTA+GGG | + | chr6_2:81018353-81018372 | Msa0931500:CDS | 35.0% | |
| GATGAATGCATAGTCAATGT+TGG | - | chr6_2:81018385-81018404 | None:intergenic | 35.0% | |
| ATTGATAGGTCAACAGAATG+AGG | - | chr6_2:81017649-81017668 | None:intergenic | 35.0% | |
| CTGTTGACCTATCAATTGTA+AGG | + | chr6_2:81017653-81017672 | Msa0931500:CDS | 35.0% | |
| GTAAGAGACTGTGATCATAA+AGG | - | chr6_2:81017750-81017769 | None:intergenic | 35.0% | |
| TTTCTTATACAGGCAATGAC+GGG | + | chr6_2:81017939-81017958 | Msa0931500:intron | 35.0% | |
| GTTTGGGAAAGATAATCTCA+CGG | + | chr6_2:81018347-81018366 | Msa0931500:CDS | 35.0% | |
| GAAAGATAATCTCACGGTTA+GGG | + | chr6_2:81018353-81018372 | Msa0931500:CDS | 35.0% | |
| GATGAATGCATAGTCAATGT+TGG | - | chr6_2:81018385-81018404 | None:intergenic | 35.0% | |
| !! | ACTCTCATAATTTGCTGCAG+AGG | - | chr6_2:81017599-81017618 | None:intergenic | 40.0% |
| TCTGCAGCAAATTATGAGAG+TGG | + | chr6_2:81017598-81017617 | Msa0931500:CDS | 40.0% | |
| GAATGAGGAACACGATATTG+AGG | - | chr6_2:81017634-81017653 | None:intergenic | 40.0% | |
| TACTTTCACCTCGAAAAGCT+TGG | - | chr6_2:81017977-81017996 | None:intergenic | 40.0% | |
| !! | AGCTTTTCGAGGTGAAAGTA+CGG | + | chr6_2:81017977-81017996 | Msa0931500:CDS | 40.0% |
| !! | CGATCTTGTGTTGTTTATGC+TGG | + | chr6_2:81018131-81018150 | Msa0931500:CDS | 40.0% |
| TATAGAAAGGGTCCTTACCT+GGG | - | chr6_2:81018177-81018196 | None:intergenic | 40.0% | |
| !! | AGTGTTCAAAGTGTTGCGTT+TGG | + | chr6_2:81018330-81018349 | Msa0931500:CDS | 40.0% |
| !! | GTGTTCAAAGTGTTGCGTTT+GGG | + | chr6_2:81018331-81018350 | Msa0931500:CDS | 40.0% |
| GGAAAGATAATCTCACGGTT+AGG | + | chr6_2:81018352-81018371 | Msa0931500:CDS | 40.0% | |
| ! | CATTGACTATGCATTCATCG+TGG | + | chr6_2:81018386-81018405 | Msa0931500:CDS | 40.0% |
| ! | GTTGTTGTCGTGTTTTGTCT+TGG | - | chr6_2:81018440-81018459 | None:intergenic | 40.0% |
| !! | ACTCTCATAATTTGCTGCAG+AGG | - | chr6_2:81017599-81017618 | None:intergenic | 40.0% |
| TCTGCAGCAAATTATGAGAG+TGG | + | chr6_2:81017598-81017617 | Msa0931500:CDS | 40.0% | |
| GAATGAGGAACACGATATTG+AGG | - | chr6_2:81017634-81017653 | None:intergenic | 40.0% | |
| TACTTTCACCTCGAAAAGCT+TGG | - | chr6_2:81017977-81017996 | None:intergenic | 40.0% | |
| !! | AGCTTTTCGAGGTGAAAGTA+CGG | + | chr6_2:81017977-81017996 | Msa0931500:CDS | 40.0% |
| !! | CGATCTTGTGTTGTTTATGC+TGG | + | chr6_2:81018131-81018150 | Msa0931500:CDS | 40.0% |
| TATAGAAAGGGTCCTTACCT+GGG | - | chr6_2:81018177-81018196 | None:intergenic | 40.0% | |
| !! | AGTGTTCAAAGTGTTGCGTT+TGG | + | chr6_2:81018330-81018349 | Msa0931500:CDS | 40.0% |
| !! | GTGTTCAAAGTGTTGCGTTT+GGG | + | chr6_2:81018331-81018350 | Msa0931500:CDS | 40.0% |
| GGAAAGATAATCTCACGGTT+AGG | + | chr6_2:81018352-81018371 | Msa0931500:CDS | 40.0% | |
| ! | CATTGACTATGCATTCATCG+TGG | + | chr6_2:81018386-81018405 | Msa0931500:CDS | 40.0% |
| ! | GTTGTTGTCGTGTTTTGTCT+TGG | - | chr6_2:81018440-81018459 | None:intergenic | 40.0% |
| AGTCTCTTACTTGATGCAGC+TGG | + | chr6_2:81017760-81017779 | Msa0931500:CDS | 45.0% | |
| ! | GATAGATGCCAAGCTTTTCG+AGG | + | chr6_2:81017966-81017985 | Msa0931500:CDS | 45.0% |
| !! | TTTTCGAGGTGAAAGTACGG+AGG | + | chr6_2:81017980-81017999 | Msa0931500:CDS | 45.0% |
| ATCTCATCACATTGTTGCCC+AGG | + | chr6_2:81018157-81018176 | Msa0931500:CDS | 45.0% | |
| ATCACATTGTTGCCCAGGTA+AGG | + | chr6_2:81018162-81018181 | Msa0931500:intron | 45.0% | |
| CTATAGAAAGGGTCCTTACC+TGG | - | chr6_2:81018178-81018197 | None:intergenic | 45.0% | |
| AGTCTCTTACTTGATGCAGC+TGG | + | chr6_2:81017760-81017779 | Msa0931500:CDS | 45.0% | |
| ! | GATAGATGCCAAGCTTTTCG+AGG | + | chr6_2:81017966-81017985 | Msa0931500:CDS | 45.0% |
| !! | TTTTCGAGGTGAAAGTACGG+AGG | + | chr6_2:81017980-81017999 | Msa0931500:CDS | 45.0% |
| ATCTCATCACATTGTTGCCC+AGG | + | chr6_2:81018157-81018176 | Msa0931500:CDS | 45.0% | |
| ATCACATTGTTGCCCAGGTA+AGG | + | chr6_2:81018162-81018181 | Msa0931500:intron | 45.0% | |
| CTATAGAAAGGGTCCTTACC+TGG | - | chr6_2:81018178-81018197 | None:intergenic | 45.0% | |
| !!! | AGATTAATATTTTATTTAAA+CGG | + | chr6_2:81017910-81017929 | Msa0931500:intron | 5.0% |
| !!! | AGATTAATATTTTATTTAAA+CGG | + | chr6_2:81017910-81017929 | Msa0931500:intron | 5.0% |
| TGTCATGACGCTGCGACGAA+AGG | + | chr6_2:81017792-81017811 | Msa0931500:CDS | 55.0% | |
| TGTCATGACGCTGCGACGAA+AGG | + | chr6_2:81017792-81017811 | Msa0931500:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 81017580 | 81018464 | 81017580 | ID=Msa0931500;Name=Msa0931500 |
| chr6_2 | mRNA | 81017580 | 81018464 | 81017580 | ID=Msa0931500-mRNA-1;Parent=Msa0931500;Name=Msa0931500-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|3|0|203 |
| chr6_2 | exon | 81017580 | 81017813 | 81017580 | ID=Msa0931500-mRNA-1:exon:14213;Parent=Msa0931500-mRNA-1 |
| chr6_2 | exon | 81017951 | 81018178 | 81017951 | ID=Msa0931500-mRNA-1:exon:14214;Parent=Msa0931500-mRNA-1 |
| chr6_2 | exon | 81018315 | 81018464 | 81018315 | ID=Msa0931500-mRNA-1:exon:14215;Parent=Msa0931500-mRNA-1 |
| chr6_2 | CDS | 81017580 | 81017813 | 81017580 | ID=Msa0931500-mRNA-1:cds;Parent=Msa0931500-mRNA-1 |
| chr6_2 | CDS | 81017951 | 81018178 | 81017951 | ID=Msa0931500-mRNA-1:cds;Parent=Msa0931500-mRNA-1 |
| chr6_2 | CDS | 81018315 | 81018464 | 81018315 | ID=Msa0931500-mRNA-1:cds;Parent=Msa0931500-mRNA-1 |
| Gene Sequence |
| Protein sequence |
