AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0933080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0933080 MtrunA17_Chr8g0360271 89.109 101 11 0 78 178 1 101 1.35e-61 192
Msa0933080 MtrunA17_Chr3g0110301 78.723 47 10 0 135 181 26 72 3.69e-20 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 82 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCGTTGGAAAAGTTTA+AGG 0.170316 6_3:+1604263 Msa0933080:three_prime_UTR
CTGAAGCATAACACCGGATT+TGG 0.286208 6_3:+1603309 Msa0933080:CDS
GATATAACCTGTATTTGTTT+AGG 0.291138 6_3:+1604358 Msa0933080:three_prime_UTR
AGAACACTGAGAAGCGAAAA+TGG 0.300690 6_3:+1601650 Msa0933080:CDS
AAAACTTTCGTTACAAAATC+AGG 0.317317 6_3:-1603108 None:intergenic
CTCGATAAGAGGAAAATAAA+AGG 0.319977 6_3:-1604534 None:intergenic
ATCTTTGCCTTTAGTTGATC+AGG 0.321053 6_3:-1603249 None:intergenic
CATCTGTGTTTGGTGGGTCT+TGG 0.324547 6_3:-1603903 None:intergenic
AGCATGGGCTCAAGTTTCTT+TGG 0.334501 6_3:+1604113 Msa0933080:three_prime_UTR
TGTAATTATGTTTGAAGTCC+AGG 0.343300 6_3:+1601556 Msa0933080:exon
AAGAAGAAGCAAGGTGTAGA+TGG 0.344753 6_3:-1601719 None:intergenic
ATGTGATGTAGACTCCTTCT+TGG 0.355967 6_3:-1604070 None:intergenic
ATCTGTGTTTGGTGGGTCTT+GGG 0.406768 6_3:-1603902 None:intergenic
CTAGTATGAAACACACCATT+TGG 0.419336 6_3:-1603171 None:intergenic
GTGTGACCATTACAAGTTGC+TGG 0.423848 6_3:+1603828 Msa0933080:three_prime_UTR
GTTACTGCAACTGAAGAATC+TGG 0.425594 6_3:+1603354 Msa0933080:CDS
TACTTATCTAGTATTTGTAT+AGG 0.429679 6_3:+1604423 Msa0933080:three_prime_UTR
GGATGGGTACTTAGGCTCTA+AGG 0.431894 6_3:+1604336 Msa0933080:three_prime_UTR
TTTCTTTGGTAAGAGCTGAT+AGG 0.431958 6_3:+1604127 Msa0933080:three_prime_UTR
AAAAGTTTAAGGATTCTGAT+TGG 0.433232 6_3:+1604274 Msa0933080:three_prime_UTR
TTATTTCCAGCAACTTGTAA+TGG 0.434209 6_3:-1603834 None:intergenic
GACATTTCAGATGATTCCTA+TGG 0.446146 6_3:+1604240 Msa0933080:three_prime_UTR
AAAACACGATACTCGATAAG+AGG 0.455221 6_3:-1604545 None:intergenic
TTGATACGGTCGATTAGTTA+TGG 0.456557 6_3:-1603226 None:intergenic
TTCAGCATTCCAGCCAAATC+CGG 0.456604 6_3:-1603322 None:intergenic
CAATACATCAACAATGGGAA+TGG 0.467040 6_3:+1603946 Msa0933080:three_prime_UTR
AAGTGTGTGGCTGCTCTCGA+TGG 0.472530 6_3:+1604210 Msa0933080:three_prime_UTR
CATCCACGCTTCCTCTCATT+AGG 0.478275 6_3:-1604318 None:intergenic
TTTCGTCATCTGTGTTTGGT+GGG 0.480692 6_3:-1603909 None:intergenic
CTGATAGGTTAAGAAGGGAT+AGG 0.484536 6_3:+1604142 Msa0933080:three_prime_UTR
AGCATAACACCGGATTTGGC+TGG 0.484895 6_3:+1603313 Msa0933080:CDS
TAGAGAAGTAAACAATCATA+AGG 0.485275 6_3:-1604448 None:intergenic
GCTGCACAGTTTCAAAAGAA+AGG 0.486109 6_3:+1603805 Msa0933080:three_prime_UTR
ATGATCAATACATCAACAAT+GGG 0.488653 6_3:+1603941 Msa0933080:three_prime_UTR
ATGGTTGATGAAGTTACAAA+AGG 0.489652 6_3:+1603141 Msa0933080:CDS
TAATCATGTGCATGTTGATA+AGG 0.492675 6_3:+1603975 Msa0933080:three_prime_UTR
ACTGGAACCTAAACAAATAC+AGG 0.492800 6_3:-1604365 None:intergenic
GTTTAAGGATTCTGATTGGA+GGG 0.496068 6_3:+1604278 Msa0933080:three_prime_UTR
ACAAAAGGAAATATGCCAAA+TGG 0.496204 6_3:+1603156 Msa0933080:CDS
ACATTAATGAGACAAAGCTT+TGG 0.497947 6_3:+1603085 Msa0933080:CDS
GAATGAGGCACTTGCAGCAT+GGG 0.501641 6_3:+1604098 Msa0933080:three_prime_UTR
TATACAAATACTAGATAAGT+AGG 0.501973 6_3:-1604421 None:intergenic
TAAGAGCTGATAGGTTAAGA+AGG 0.504282 6_3:+1604136 Msa0933080:three_prime_UTR
GTAATTATGTTTGAAGTCCA+GGG 0.509433 6_3:+1601557 Msa0933080:exon
TTTAGTTGATCAGGTTGATA+CGG 0.511505 6_3:-1603240 None:intergenic
AATACATCAACAATGGGAAT+GGG 0.512612 6_3:+1603947 Msa0933080:three_prime_UTR
AAAAGTGAAAGAGCTAAAAT+GGG 0.512721 6_3:+1601434 Msa0933080:five_prime_UTR
CTGCACAGTTTCAAAAGAAA+GGG 0.519886 6_3:+1603806 Msa0933080:three_prime_UTR
TTATTGTTACTTAGTATGAC+AGG 0.524867 6_3:+1604574 Msa0933080:three_prime_UTR
TGAATAGAACTCACGATACA+TGG 0.530708 6_3:-1603286 None:intergenic
AAGAGCTGATAGGTTAAGAA+GGG 0.537119 6_3:+1604137 Msa0933080:three_prime_UTR
GGAAGCGTGGATGGGTACTT+AGG 0.537157 6_3:+1604328 Msa0933080:three_prime_UTR
CTAATGAGAGGAAGCGTGGA+TGG 0.541911 6_3:+1604319 Msa0933080:three_prime_UTR
TGATAAGTATGTCTCATCAC+TGG 0.544625 6_3:-1604383 None:intergenic
GATGATCAATACATCAACAA+TGG 0.551531 6_3:+1603940 Msa0933080:three_prime_UTR
CACTCGCAGTGAAAAGCGAC+AGG 0.557370 6_3:+1604035 Msa0933080:three_prime_UTR
GTATCAACCTGATCAACTAA+AGG 0.566076 6_3:+1603242 Msa0933080:CDS
AGTTTAAGGATTCTGATTGG+AGG 0.570217 6_3:+1604277 Msa0933080:three_prime_UTR
ATCGAGAGCAGCCACACACT+TGG 0.570899 6_3:-1604208 None:intergenic
AGTGTGTGGCTGCTCTCGAT+GGG 0.579630 6_3:+1604211 Msa0933080:three_prime_UTR
AACTAAAGGCAAAGATGCAT+AGG 0.582306 6_3:+1603256 Msa0933080:CDS
TGATTCCTATGGAAAAGCGT+TGG 0.584496 6_3:+1604251 Msa0933080:three_prime_UTR
AGCGAAAATGGCGATTTCGA+CGG 0.590473 6_3:+1601662 Msa0933080:CDS
TACATCACATCATATGAATG+AGG 0.591772 6_3:+1604083 Msa0933080:three_prime_UTR
TAATGAGAGGAAGCGTGGAT+GGG 0.591816 6_3:+1604320 Msa0933080:three_prime_UTR
TGAATGAGGCACTTGCAGCA+TGG 0.593350 6_3:+1604097 Msa0933080:three_prime_UTR
TTGTTCTCTTACCAAGTGTG+TGG 0.604130 6_3:+1604197 Msa0933080:three_prime_UTR
TAATTATGTTTGAAGTCCAG+GGG 0.605620 6_3:+1601558 Msa0933080:exon
ACGCTTCCTCTCATTAGGCA+TGG 0.615135 6_3:-1604313 None:intergenic
ACCGTGCTTCTTCTTCTCTG+CGG 0.616187 6_3:+1601762 Msa0933080:CDS
GGTTCATGTGAGATCCAAGA+AGG 0.621915 6_3:+1604056 Msa0933080:three_prime_UTR
ACCGCAGAGAAGAAGAAGCA+CGG 0.624330 6_3:-1601763 None:intergenic
ACTCAACTTATACATCATTG+AGG 0.624896 6_3:-1603202 None:intergenic
ATGCCTAATGAGAGGAAGCG+TGG 0.638032 6_3:+1604315 Msa0933080:three_prime_UTR
AAGAAGGGATAGGAGTGCAG+AGG 0.647414 6_3:+1604152 Msa0933080:three_prime_UTR
GTGTGTTTCATACTAGAACA+TGG 0.658064 6_3:+1603178 Msa0933080:CDS
AAATAAGAAAAGTGAAAACA+CGG 0.659306 6_3:+1601502 Msa0933080:five_prime_UTR
GCGGTGGAGAAGAAGAAGCA+AGG 0.660951 6_3:-1601728 None:intergenic
ACGGTAGAGAAGAAGCGCGG+TGG 0.673948 6_3:-1601744 None:intergenic
TTATCGCCATGCCTAATGAG+AGG 0.675722 6_3:+1604307 Msa0933080:three_prime_UTR
TATTCACTGAAGCATAACAC+CGG 0.679933 6_3:+1603303 Msa0933080:CDS
AGCACGGTAGAGAAGAAGCG+CGG 0.713678 6_3:-1601747 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTTTTTTTTATCTAAAC+AGG - chr6_3:1602097-1602116 None:intergenic 15.0%
!! TTAAATGCTTATTCAATTAA+TGG - chr6_3:1602646-1602665 None:intergenic 15.0%
!! TGATTATATATATACCATAT+TGG - chr6_3:1603531-1603550 None:intergenic 15.0%
!! ATGGTATATATATAATCATT+TGG + chr6_3:1603533-1603552 Msa0933080:intron 15.0%
!! AAATAAGAAAAGTGAAAACA+CGG + chr6_3:1601502-1601521 Msa0933080:five_prime_UTR 20.0%
!! AGTAAAAAAATCGCTATATT+AGG - chr6_3:1601865-1601884 None:intergenic 20.0%
!! AAAAACACAAAATCATAGAA+CGG - chr6_3:1601917-1601936 None:intergenic 20.0%
!!! TCTATGTAAATTTAAGATTG+CGG + chr6_3:1601941-1601960 Msa0933080:intron 20.0%
!!! TTGAATTTTACCACTTTTAA+TGG + chr6_3:1603403-1603422 Msa0933080:intron 20.0%
!!! TGAATTTTACCACTTTTAAT+GGG + chr6_3:1603404-1603423 Msa0933080:intron 20.0%
!! ATTTATCATAATTCCAACAA+GGG - chr6_3:1603508-1603527 None:intergenic 20.0%
!! GAATTATGATAAATCCAATA+TGG + chr6_3:1603514-1603533 Msa0933080:intron 20.0%
!! TATAATCATTTGGTACATAT+TGG + chr6_3:1603543-1603562 Msa0933080:intron 20.0%
!! AAATACAAACAAACACAATA+TGG - chr6_3:1603657-1603676 None:intergenic 20.0%
!! TATACAAATACTAGATAAGT+AGG - chr6_3:1604424-1604443 None:intergenic 20.0%
!! TACTTATCTAGTATTTGTAT+AGG + chr6_3:1604423-1604442 Msa0933080:three_prime_UTR 20.0%
! AAAAAGTGAAAGAGCTAAAA+TGG + chr6_3:1601433-1601452 Msa0933080:five_prime_UTR 25.0%
! AAAAGTGAAAGAGCTAAAAT+GGG + chr6_3:1601434-1601453 Msa0933080:five_prime_UTR 25.0%
! TAAAAAAATGATAATACCCC+TGG - chr6_3:1601577-1601596 None:intergenic 25.0%
! TGTATCTTTCAATATTCACA+CGG + chr6_3:1602355-1602374 Msa0933080:intron 25.0%
! AAATATGTAGCCATCTATTA+GGG - chr6_3:1602545-1602564 None:intergenic 25.0%
!! CAAAAATATTCGTGTTTTCT+CGG + chr6_3:1602835-1602854 Msa0933080:intron 25.0%
! GACTAAACTGTAAAAATAGA+AGG - chr6_3:1602891-1602910 None:intergenic 25.0%
!!! ATGCGTATTTTTATGATAGA+TGG + chr6_3:1603050-1603069 Msa0933080:intron 25.0%
! AAAACTTTCGTTACAAAATC+AGG - chr6_3:1603111-1603130 None:intergenic 25.0%
!! GAAAGTTTTCATTGACATAA+TGG + chr6_3:1603122-1603141 Msa0933080:CDS 25.0%
! AATCTGGAAAAATTATCTGA+AGG + chr6_3:1603370-1603389 Msa0933080:CDS 25.0%
! GATTTATCATAATTCCAACA+AGG - chr6_3:1603509-1603528 None:intergenic 25.0%
!!! TTTCATTGATTCTCTTACTT+AGG + chr6_3:1603720-1603739 Msa0933080:intron 25.0%
! ATACAATGAGAATAACAGAA+AGG - chr6_3:1603748-1603767 None:intergenic 25.0%
! TGATATGCATATATCATTTC+AGG + chr6_3:1603771-1603790 Msa0933080:intron 25.0%
! ATGATCAATACATCAACAAT+GGG + chr6_3:1603941-1603960 Msa0933080:three_prime_UTR 25.0%
! AAAAGTTTAAGGATTCTGAT+TGG + chr6_3:1604274-1604293 Msa0933080:three_prime_UTR 25.0%
! GATATAACCTGTATTTGTTT+AGG + chr6_3:1604358-1604377 Msa0933080:three_prime_UTR 25.0%
! TAGAGAAGTAAACAATCATA+AGG - chr6_3:1604451-1604470 None:intergenic 25.0%
!!! AAAAGTGTCACTTTTCTCAA+TGG + chr6_3:1601527-1601546 Msa0933080:five_prime_UTR 30.0%
! TGTAATTATGTTTGAAGTCC+AGG + chr6_3:1601556-1601575 Msa0933080:exon 30.0%
! GTAATTATGTTTGAAGTCCA+GGG + chr6_3:1601557-1601576 Msa0933080:exon 30.0%
! TAATTATGTTTGAAGTCCAG+GGG + chr6_3:1601558-1601577 Msa0933080:exon 30.0%
!! TTAGTTTTTCTGCAGAATGA+GGG - chr6_3:1601626-1601645 None:intergenic 30.0%
TGTAGATGGAAGAAAAATCA+CGG - chr6_3:1601708-1601727 None:intergenic 30.0%
GATTGATATTTGCAGCTTAT+AGG + chr6_3:1602058-1602077 Msa0933080:intron 30.0%
CAAATATGTAGCCATCTATT+AGG - chr6_3:1602546-1602565 None:intergenic 30.0%
TAGATGGCTACATATTTGTT+TGG + chr6_3:1602548-1602567 Msa0933080:intron 30.0%
! TTTACTTGTACAACCCATTT+TGG - chr6_3:1602721-1602740 None:intergenic 30.0%
TTTACTAACAAGACAAGAAG+TGG - chr6_3:1602756-1602775 None:intergenic 30.0%
AGAGAGAGCAAATAAAATCA+AGG - chr6_3:1602945-1602964 None:intergenic 30.0%
!! TTGATTTTATTTGCTCTCTC+TGG + chr6_3:1602944-1602963 Msa0933080:intron 30.0%
ACATTAATGAGACAAAGCTT+TGG + chr6_3:1603085-1603104 Msa0933080:CDS 30.0%
!! ATGGTTGATGAAGTTACAAA+AGG + chr6_3:1603141-1603160 Msa0933080:CDS 30.0%
ACAAAAGGAAATATGCCAAA+TGG + chr6_3:1603156-1603175 Msa0933080:CDS 30.0%
ACTCAACTTATACATCATTG+AGG - chr6_3:1603205-1603224 None:intergenic 30.0%
!! TTTAGTTGATCAGGTTGATA+CGG - chr6_3:1603243-1603262 None:intergenic 30.0%
GGAAAAATTATCTGAAGGTA+TGG + chr6_3:1603375-1603394 Msa0933080:intron 30.0%
ATCATATCACCCATTAAAAG+TGG - chr6_3:1603416-1603435 None:intergenic 30.0%
TTAGAGCAATGAACAAAACT+GGG - chr6_3:1603588-1603607 None:intergenic 30.0%
! TTTAGAGCAATGAACAAAAC+TGG - chr6_3:1603589-1603608 None:intergenic 30.0%
CAAGATTCATGATTCTAGAT+AGG - chr6_3:1603682-1603701 None:intergenic 30.0%
TTATTTCCAGCAACTTGTAA+TGG - chr6_3:1603837-1603856 None:intergenic 30.0%
TTCAACAAAAACAATGCAAC+TGG + chr6_3:1603859-1603878 Msa0933080:three_prime_UTR 30.0%
GATGATCAATACATCAACAA+TGG + chr6_3:1603940-1603959 Msa0933080:three_prime_UTR 30.0%
AATACATCAACAATGGGAAT+GGG + chr6_3:1603947-1603966 Msa0933080:three_prime_UTR 30.0%
TAATCATGTGCATGTTGATA+AGG + chr6_3:1603975-1603994 Msa0933080:three_prime_UTR 30.0%
TACATCACATCATATGAATG+AGG + chr6_3:1604083-1604102 Msa0933080:three_prime_UTR 30.0%
!! AAAAGCGTTGGAAAAGTTTA+AGG + chr6_3:1604263-1604282 Msa0933080:three_prime_UTR 30.0%
CTCGATAAGAGGAAAATAAA+AGG - chr6_3:1604537-1604556 None:intergenic 30.0%
!!! GTTAGTTTTTCTGCAGAATG+AGG - chr6_3:1601627-1601646 None:intergenic 35.0%
ATATTTGCAGCTTATAGGTC+TGG + chr6_3:1602063-1602082 Msa0933080:intron 35.0%
TTCAATATTCACACGGATAC+CGG + chr6_3:1602362-1602381 Msa0933080:intron 35.0%
TGCACACAAGAACTAGAAAA+AGG - chr6_3:1602508-1602527 None:intergenic 35.0%
TATGCTTGTTCCCTAATAGA+TGG + chr6_3:1602532-1602551 Msa0933080:intron 35.0%
GTTTGAGCTCAATACTTCAA+AGG - chr6_3:1602691-1602710 None:intergenic 35.0%
TCAAACAGCTTAGCCAAAAT+GGG + chr6_3:1602705-1602724 Msa0933080:intron 35.0%
GGTTGTACAAGTAAAGAACA+GGG + chr6_3:1602726-1602745 Msa0933080:intron 35.0%
GTTGTACAAGTAAAGAACAG+GGG + chr6_3:1602727-1602746 Msa0933080:intron 35.0%
! TCTTGTCTTGTTAGTAAAGC+TGG + chr6_3:1602758-1602777 Msa0933080:intron 35.0%
CAAGACTTGGCACAAAAATA+AGG - chr6_3:1602799-1602818 None:intergenic 35.0%
TATCTGTCAGATACAAGACT+TGG - chr6_3:1602812-1602831 None:intergenic 35.0%
CTAGTATGAAACACACCATT+TGG - chr6_3:1603174-1603193 None:intergenic 35.0%
GTGTGTTTCATACTAGAACA+TGG + chr6_3:1603178-1603197 Msa0933080:CDS 35.0%
! TTGATACGGTCGATTAGTTA+TGG - chr6_3:1603229-1603248 None:intergenic 35.0%
GTATCAACCTGATCAACTAA+AGG + chr6_3:1603242-1603261 Msa0933080:CDS 35.0%
ATCTTTGCCTTTAGTTGATC+AGG - chr6_3:1603252-1603271 None:intergenic 35.0%
AACTAAAGGCAAAGATGCAT+AGG + chr6_3:1603256-1603275 Msa0933080:CDS 35.0%
TGAATAGAACTCACGATACA+TGG - chr6_3:1603289-1603308 None:intergenic 35.0%
TATTCACTGAAGCATAACAC+CGG + chr6_3:1603303-1603322 Msa0933080:CDS 35.0%
TCATGCTTCTATTAGTGACA+TGG + chr6_3:1603461-1603480 Msa0933080:intron 35.0%
TCACATTTCAATACCCTTGT+TGG + chr6_3:1603492-1603511 Msa0933080:intron 35.0%
!!! TTTTGAAACTGTGCAGCTTT+GGG - chr6_3:1603802-1603821 None:intergenic 35.0%
CTGCACAGTTTCAAAAGAAA+GGG + chr6_3:1603806-1603825 Msa0933080:three_prime_UTR 35.0%
CAATACATCAACAATGGGAA+TGG + chr6_3:1603946-1603965 Msa0933080:three_prime_UTR 35.0%
TTTCTTTGGTAAGAGCTGAT+AGG + chr6_3:1604127-1604146 Msa0933080:three_prime_UTR 35.0%
TAAGAGCTGATAGGTTAAGA+AGG + chr6_3:1604136-1604155 Msa0933080:three_prime_UTR 35.0%
AAGAGCTGATAGGTTAAGAA+GGG + chr6_3:1604137-1604156 Msa0933080:three_prime_UTR 35.0%
GACATTTCAGATGATTCCTA+TGG + chr6_3:1604240-1604259 Msa0933080:three_prime_UTR 35.0%
AGTTTAAGGATTCTGATTGG+AGG + chr6_3:1604277-1604296 Msa0933080:three_prime_UTR 35.0%
GTTTAAGGATTCTGATTGGA+GGG + chr6_3:1604278-1604297 Msa0933080:three_prime_UTR 35.0%
ACTGGAACCTAAACAAATAC+AGG - chr6_3:1604368-1604387 None:intergenic 35.0%
TGATAAGTATGTCTCATCAC+TGG - chr6_3:1604386-1604405 None:intergenic 35.0%
AAAACACGATACTCGATAAG+AGG - chr6_3:1604548-1604567 None:intergenic 35.0%
AGAACACTGAGAAGCGAAAA+TGG + chr6_3:1601650-1601669 Msa0933080:CDS 40.0%
AGATGGAAGAAAAATCACGG+CGG - chr6_3:1601705-1601724 None:intergenic 40.0%
AAGAAGAAGCAAGGTGTAGA+TGG - chr6_3:1601722-1601741 None:intergenic 40.0%
!!! TTCTTCTCTGCGGTTTTTTC+AGG + chr6_3:1601772-1601791 Msa0933080:CDS 40.0%
CTCAAACAGCTTAGCCAAAA+TGG + chr6_3:1602704-1602723 Msa0933080:intron 40.0%
GGGTTGTACAAGTAAAGAAC+AGG + chr6_3:1602725-1602744 Msa0933080:intron 40.0%
GCACAAAAATAAGGCTACCT+AGG - chr6_3:1602790-1602809 None:intergenic 40.0%
!!! CTCTCTGGTGCTACTTTTAT+AGG + chr6_3:1602959-1602978 Msa0933080:intron 40.0%
GTTACTGCAACTGAAGAATC+TGG + chr6_3:1603354-1603373 Msa0933080:CDS 40.0%
!!! CTTTTGAAACTGTGCAGCTT+TGG - chr6_3:1603803-1603822 None:intergenic 40.0%
GCTGCACAGTTTCAAAAGAA+AGG + chr6_3:1603805-1603824 Msa0933080:three_prime_UTR 40.0%
TTTCGTCATCTGTGTTTGGT+GGG - chr6_3:1603912-1603931 None:intergenic 40.0%
! TTTTCGTCATCTGTGTTTGG+TGG - chr6_3:1603913-1603932 None:intergenic 40.0%
! TCGTTTTCGTCATCTGTGTT+TGG - chr6_3:1603916-1603935 None:intergenic 40.0%
ATGTGATGTAGACTCCTTCT+TGG - chr6_3:1604073-1604092 None:intergenic 40.0%
CTGATAGGTTAAGAAGGGAT+AGG + chr6_3:1604142-1604161 Msa0933080:three_prime_UTR 40.0%
TTGTTCTCTTACCAAGTGTG+TGG + chr6_3:1604197-1604216 Msa0933080:three_prime_UTR 40.0%
! TGATTCCTATGGAAAAGCGT+TGG + chr6_3:1604251-1604270 Msa0933080:three_prime_UTR 40.0%
! CTTTTCCAACGCTTTTCCAT+AGG - chr6_3:1604259-1604278 None:intergenic 40.0%
AGCGAAAATGGCGATTTCGA+CGG + chr6_3:1601662-1601681 Msa0933080:CDS 45.0%
AGAAAAATCACGGCGGAGAT+CGG - chr6_3:1601698-1601717 None:intergenic 45.0%
!! CCGTTGACGAGTTTTGTATC+CGG - chr6_3:1602384-1602403 None:intergenic 45.0%
CCGGATACAAAACTCGTCAA+CGG + chr6_3:1602381-1602400 Msa0933080:intron 45.0%
AGTAAAGCTGGACTGAACCT+AGG + chr6_3:1602770-1602789 Msa0933080:intron 45.0%
CTGAAGCATAACACCGGATT+TGG + chr6_3:1603309-1603328 Msa0933080:CDS 45.0%
TTCAGCATTCCAGCCAAATC+CGG - chr6_3:1603325-1603344 None:intergenic 45.0%
GTGTGACCATTACAAGTTGC+TGG + chr6_3:1603828-1603847 Msa0933080:three_prime_UTR 45.0%
! ATCTGTGTTTGGTGGGTCTT+GGG - chr6_3:1603905-1603924 None:intergenic 45.0%
GGTTCATGTGAGATCCAAGA+AGG + chr6_3:1604056-1604075 Msa0933080:three_prime_UTR 45.0%
AGCATGGGCTCAAGTTTCTT+TGG + chr6_3:1604113-1604132 Msa0933080:three_prime_UTR 45.0%
TTATCGCCATGCCTAATGAG+AGG + chr6_3:1604307-1604326 Msa0933080:three_prime_UTR 45.0%
! TAATGAGAGGAAGCGTGGAT+GGG + chr6_3:1604320-1604339 Msa0933080:three_prime_UTR 45.0%
!!! ATTTTTTTATCAAAAAAAAT+TGG + chr6_3:1601588-1601607 Msa0933080:CDS 5.0%
ACCGTGCTTCTTCTTCTCTG+CGG + chr6_3:1601762-1601781 Msa0933080:CDS 50.0%
ACCGCAGAGAAGAAGAAGCA+CGG - chr6_3:1601766-1601785 None:intergenic 50.0%
AGCATAACACCGGATTTGGC+TGG + chr6_3:1603313-1603332 Msa0933080:CDS 50.0%
! CATCTGTGTTTGGTGGGTCT+TGG - chr6_3:1603906-1603925 None:intergenic 50.0%
! TGAATGAGGCACTTGCAGCA+TGG + chr6_3:1604097-1604116 Msa0933080:three_prime_UTR 50.0%
! GAATGAGGCACTTGCAGCAT+GGG + chr6_3:1604098-1604117 Msa0933080:three_prime_UTR 50.0%
! AAGAAGGGATAGGAGTGCAG+AGG + chr6_3:1604152-1604171 Msa0933080:three_prime_UTR 50.0%
ACGCTTCCTCTCATTAGGCA+TGG - chr6_3:1604316-1604335 None:intergenic 50.0%
ATGCCTAATGAGAGGAAGCG+TGG + chr6_3:1604315-1604334 Msa0933080:three_prime_UTR 50.0%
CATCCACGCTTCCTCTCATT+AGG - chr6_3:1604321-1604340 None:intergenic 50.0%
! CTAATGAGAGGAAGCGTGGA+TGG + chr6_3:1604319-1604338 Msa0933080:three_prime_UTR 50.0%
GGATGGGTACTTAGGCTCTA+AGG + chr6_3:1604336-1604355 Msa0933080:three_prime_UTR 50.0%
GCGGTGGAGAAGAAGAAGCA+AGG - chr6_3:1601731-1601750 None:intergenic 55.0%
AGCACGGTAGAGAAGAAGCG+CGG - chr6_3:1601750-1601769 None:intergenic 55.0%
CACTCGCAGTGAAAAGCGAC+AGG + chr6_3:1604035-1604054 Msa0933080:three_prime_UTR 55.0%
ATCGAGAGCAGCCACACACT+TGG - chr6_3:1604211-1604230 None:intergenic 55.0%
AAGTGTGTGGCTGCTCTCGA+TGG + chr6_3:1604210-1604229 Msa0933080:three_prime_UTR 55.0%
AGTGTGTGGCTGCTCTCGAT+GGG + chr6_3:1604211-1604230 Msa0933080:three_prime_UTR 55.0%
! GGAAGCGTGGATGGGTACTT+AGG + chr6_3:1604328-1604347 Msa0933080:three_prime_UTR 55.0%
! ACGGTAGAGAAGAAGCGCGG+TGG - chr6_3:1601747-1601766 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 1601432 1604593 1601432 ID=Msa0933080;Name=Msa0933080
chr6_3 mRNA 1601432 1604593 1601432 ID=Msa0933080-mRNA-1;Parent=Msa0933080;Name=Msa0933080-mRNA-1;_AED=0.21;_eAED=0.21;_QI=131|1|1|1|0.5|0.66|3|803|183
chr6_3 exon 1601432 1601793 1601432 ID=Msa0933080-mRNA-1:exon:619;Parent=Msa0933080-mRNA-1
chr6_3 exon 1603069 1603391 1603069 ID=Msa0933080-mRNA-1:exon:620;Parent=Msa0933080-mRNA-1
chr6_3 exon 1603793 1604593 1603793 ID=Msa0933080-mRNA-1:exon:621;Parent=Msa0933080-mRNA-1
chr6_3 five_prime_UTR 1601432 1601562 1601432 ID=Msa0933080-mRNA-1:five_prime_utr;Parent=Msa0933080-mRNA-1
chr6_3 CDS 1601563 1601793 1601563 ID=Msa0933080-mRNA-1:cds;Parent=Msa0933080-mRNA-1
chr6_3 CDS 1603069 1603389 1603069 ID=Msa0933080-mRNA-1:cds;Parent=Msa0933080-mRNA-1
chr6_3 three_prime_UTR 1603390 1603391 1603390 ID=Msa0933080-mRNA-1:three_prime_utr;Parent=Msa0933080-mRNA-1
chr6_3 three_prime_UTR 1603793 1604593 1603793 ID=Msa0933080-mRNA-1:three_prime_utr;Parent=Msa0933080-mRNA-1
Gene Sequence

>Msa0933080

ATGTTTGAAGTCCAGGGGTATTATCATTTTTTTATCAAAAAAAATTGGATCAAAAATTATCCCTCATTCTGCAGAAAAACTAACGTGAGAACACTGAGAAGCGAAAATGGCGATTTCGACGGCGATTCTTCTCCGATCTCCGCCGTGATTTTTCTTCCATCTACACCTTGCTTCTTCTTCTCCACCGCGCTTCTTCTCTACCGTGCTTCTTCTTCTCTGCGGTTTTTTCAGATGGCAACTAATGTTGACATTAATGAGACAAAGCTTTGGCCTGATTTTGTAACGAAAGTTTTCATTGACATAATGGTTGATGAAGTTACAAAAGGAAATATGCCAAATGGTGTGTTTCATACTAGAACATGGTCCTCAATGATGTATAAGTTGAGTACCATAACTAATCGACCGTATCAACCTGATCAACTAAAGGCAAAGATGCATAGGTTGCGAGCCATGTATCGTGAGTTCTATTCACTGAAGCATAACACCGGATTTGGCTGGAATGCTGAAACAAACGCTGTTACTGCAACTGAAGAATCTGGAAAAATTATCTGA
Protein sequence

>Msa0933080

MFEVQGYYHFFIKKNWIKNYPSFCRKTNVRTLRSENGDFDGDSSPISAVIFLPSTPCFFFSTALLLYRASSSLRFFQMATNVDINETKLWPDFVTKVFIDIMVDEVTKGNMPNGVFHTRTWSSMMYKLSTITNRPYQPDQLKAKMHRLRAMYREFYSLKHNTGFGWNAETNAVTATEESGKII*