AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0954810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGGTTTAAGAACAATTT+TGG 0.111013 6_3:+57320829 None:intergenic
TTTAGGTTTGGAAAACATTT+TGG 0.113465 6_3:+57320893 None:intergenic
GGCTATTAGGGTTCAATTTC+GGG 0.171813 6_3:-57318690 Msa0954810:three_prime_UTR
CAACAAAAGCATCGCAAATT+TGG 0.177563 6_3:+57320800 None:intergenic
ATGCAATGAGGTTGGCTATT+AGG 0.183562 6_3:-57318703 Msa0954810:three_prime_UTR
GGTTTATGAACAACACAAAT+TGG 0.228395 6_3:+57320866 None:intergenic
TCGCAAATTTGGAGCGATTA+AGG 0.236402 6_3:+57320811 None:intergenic
TGCAATGAGGTTGGCTATTA+GGG 0.256824 6_3:-57318702 Msa0954810:three_prime_UTR
GAAATCGCGTTTGTGTGTTT+TGG 0.257983 6_3:+57321011 None:intergenic
TGGCTATTAGGGTTCAATTT+CGG 0.266498 6_3:-57318691 Msa0954810:three_prime_UTR
TCGGGAAGTTTATGAGTATT+GGG 0.323809 6_3:-57318672 Msa0954810:three_prime_UTR
CGCAAATTTGGAGCGATTAA+GGG 0.328549 6_3:+57320812 None:intergenic
TTCGGGAAGTTTATGAGTAT+TGG 0.347894 6_3:-57318673 Msa0954810:three_prime_UTR
TGTTCAATTTATTCTCAAAC+TGG 0.364651 6_3:-57320680 Msa0954810:intron
TGAAGTGATTCTACGTTTCA+TGG 0.373510 6_3:+57320973 None:intergenic
TCAATGGTTTCCAACACTTT+CGG 0.396534 6_3:-57318633 Msa0954810:three_prime_UTR
GTTTATGAACAACACAAATT+GGG 0.408847 6_3:+57320867 None:intergenic
AAAATGTTCCTCAAGATCAA+TGG 0.447604 6_3:-57318649 Msa0954810:three_prime_UTR
TCGAACAGAAAGTAGCTTGA+AGG 0.477783 6_3:+57320732 None:intergenic
TATATCCTTACCGAAAGTGT+TGG 0.496173 6_3:+57318623 None:intergenic
CAGTGTTGCACAATGAATAG+AGG 0.510642 6_3:+57318586 None:intergenic
TGGGAAGTGATTATGAGTGA+TGG 0.516055 6_3:+57320913 None:intergenic
GGTTTCCAACACTTTCGGTA+AGG 0.516699 6_3:-57318628 Msa0954810:three_prime_UTR
GGGAAGTGATTATGAGTGAT+GGG 0.517301 6_3:+57320914 None:intergenic
GTTGGAAACCATTGATCTTG+AGG 0.534071 6_3:+57318641 None:intergenic
TCTACGTTTCATGGTTTGAG+AGG 0.545321 6_3:+57320982 None:intergenic
TGCTGCTCATGCAATGAGGT+TGG 0.575123 6_3:-57318711 Msa0954810:three_prime_UTR
AAGTAGCTTGAAGGCAGCAA+CGG 0.603239 6_3:+57320741 None:intergenic
AAGATGCTGCTCATGCAATG+AGG 0.725690 6_3:-57318715 Msa0954810:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATTCAATTTAATCT+TGG + chr6_3:57319602-57319621 None:intergenic 10.0%
!! TTAAATTGAATTTAAATAAG+AGG - chr6_3:57319605-57319624 Msa0954810:intron 10.0%
!!! CAATATTTTCAATATATATT+AGG + chr6_3:57320354-57320373 None:intergenic 10.0%
!!! ATATCCATTTTAATTATATT+AGG + chr6_3:57320396-57320415 None:intergenic 10.0%
!! ATATAATTAAAATGGATATA+AGG - chr6_3:57320397-57320416 Msa0954810:intron 10.0%
!! ATTTAAATTCAATTTAATCT+TGG + chr6_3:57319602-57319621 None:intergenic 10.0%
!! TTAAATTGAATTTAAATAAG+AGG - chr6_3:57319605-57319624 Msa0954810:intron 10.0%
!!! CAATATTTTCAATATATATT+AGG + chr6_3:57320354-57320373 None:intergenic 10.0%
!!! ATATCCATTTTAATTATATT+AGG + chr6_3:57320396-57320415 None:intergenic 10.0%
!! ATATAATTAAAATGGATATA+AGG - chr6_3:57320397-57320416 Msa0954810:intron 10.0%
!!! ATTATAGTTTGTTTATTGTT+TGG - chr6_3:57319057-57319076 Msa0954810:intron 15.0%
!!! TACTTTTTGTATCTATTTTA+AGG - chr6_3:57319429-57319448 Msa0954810:intron 15.0%
!! TTAATCAAAAACAAAAGAAA+AGG + chr6_3:57319549-57319568 None:intergenic 15.0%
!!! GTTTTATTTTTCAAAATTAG+TGG - chr6_3:57319731-57319750 Msa0954810:intron 15.0%
!!! ATTTTAATTATATTAGGTAG+TGG + chr6_3:57320390-57320409 None:intergenic 15.0%
!! ACTACCTAATATAATTAAAA+TGG - chr6_3:57320389-57320408 Msa0954810:intron 15.0%
!!! ATTATAGTTTGTTTATTGTT+TGG - chr6_3:57319057-57319076 Msa0954810:intron 15.0%
!!! TACTTTTTGTATCTATTTTA+AGG - chr6_3:57319429-57319448 Msa0954810:intron 15.0%
!! TTAATCAAAAACAAAAGAAA+AGG + chr6_3:57319549-57319568 None:intergenic 15.0%
!!! GTTTTATTTTTCAAAATTAG+TGG - chr6_3:57319731-57319750 Msa0954810:intron 15.0%
!!! ATTTTAATTATATTAGGTAG+TGG + chr6_3:57320390-57320409 None:intergenic 15.0%
!! ACTACCTAATATAATTAAAA+TGG - chr6_3:57320389-57320408 Msa0954810:intron 15.0%
!!! GATTTGTAAAGATGTTTTAA+TGG - chr6_3:57319009-57319028 Msa0954810:intron 20.0%
!! CAGAAGAAAAAAAAAACAAT+GGG + chr6_3:57319140-57319159 None:intergenic 20.0%
!! TCAGAAGAAAAAAAAAACAA+TGG + chr6_3:57319141-57319160 None:intergenic 20.0%
!!! GCATTTTGTTTGATATTTTA+GGG - chr6_3:57319451-57319470 Msa0954810:intron 20.0%
!!! TTTTTATGAGCAAAACATAT+AGG - chr6_3:57320245-57320264 Msa0954810:intron 20.0%
!!! ATTTTAATGCTTTTGTATGT+TGG + chr6_3:57320277-57320296 None:intergenic 20.0%
!!! AACATACAAAAGCATTAAAA+TGG - chr6_3:57320276-57320295 Msa0954810:intron 20.0%
!! TAATTAAAATGGATATAAGG+TGG - chr6_3:57320400-57320419 Msa0954810:intron 20.0%
!!! TCAAAATAAATTTAGGTTAG+TGG + chr6_3:57320426-57320445 None:intergenic 20.0%
!! CAAGATCAGAAATATATATT+AGG + chr6_3:57320505-57320524 None:intergenic 20.0%
!! ATATGATGACTATGAATATA+TGG - chr6_3:57320739-57320758 Msa0954810:CDS 20.0%
!! TTCTAGTAATGTTACAATTT+CGG - chr6_3:57320834-57320853 Msa0954810:five_prime_UTR 20.0%
!!! GATTTGTAAAGATGTTTTAA+TGG - chr6_3:57319009-57319028 Msa0954810:intron 20.0%
!! CAGAAGAAAAAAAAAACAAT+GGG + chr6_3:57319140-57319159 None:intergenic 20.0%
!! TCAGAAGAAAAAAAAAACAA+TGG + chr6_3:57319141-57319160 None:intergenic 20.0%
!!! GCATTTTGTTTGATATTTTA+GGG - chr6_3:57319451-57319470 Msa0954810:intron 20.0%
!!! TTTTTATGAGCAAAACATAT+AGG - chr6_3:57320245-57320264 Msa0954810:intron 20.0%
!!! ATTTTAATGCTTTTGTATGT+TGG + chr6_3:57320277-57320296 None:intergenic 20.0%
!!! AACATACAAAAGCATTAAAA+TGG - chr6_3:57320276-57320295 Msa0954810:intron 20.0%
!! TAATTAAAATGGATATAAGG+TGG - chr6_3:57320400-57320419 Msa0954810:intron 20.0%
!!! TCAAAATAAATTTAGGTTAG+TGG + chr6_3:57320426-57320445 None:intergenic 20.0%
!! CAAGATCAGAAATATATATT+AGG + chr6_3:57320505-57320524 None:intergenic 20.0%
!! ATATGATGACTATGAATATA+TGG - chr6_3:57320739-57320758 Msa0954810:CDS 20.0%
!! TTCTAGTAATGTTACAATTT+CGG - chr6_3:57320834-57320853 Msa0954810:five_prime_UTR 20.0%
!! TTAGGTTTGGAAAACATTTT+GGG + chr6_3:57318731-57318750 None:intergenic 25.0%
!!! TTTAGGTTTGGAAAACATTT+TGG + chr6_3:57318732-57318751 None:intergenic 25.0%
! GTTTATGAACAACACAAATT+GGG + chr6_3:57318758-57318777 None:intergenic 25.0%
!!! ATTTTGGGATTGATTTTGTT+AGG + chr6_3:57318780-57318799 None:intergenic 25.0%
!! AAGGGTTTAAGAACAATTTT+GGG + chr6_3:57318795-57318814 None:intergenic 25.0%
!! TAAGGGTTTAAGAACAATTT+TGG + chr6_3:57318796-57318815 None:intergenic 25.0%
! TGTTCAATTTATTCTCAAAC+TGG - chr6_3:57318942-57318961 Msa0954810:intron 25.0%
!! GAAAAGTTATGAGCTTTATA+TGG - chr6_3:57319209-57319228 Msa0954810:intron 25.0%
! TTTAATCAAACAAGATCCAA+TGG + chr6_3:57319357-57319376 None:intergenic 25.0%
!! TTGGATCTTGTTTGATTAAA+AGG - chr6_3:57319357-57319376 Msa0954810:intron 25.0%
!! TGGATCTTGTTTGATTAAAA+GGG - chr6_3:57319358-57319377 Msa0954810:intron 25.0%
!!! GGCATTTTGTTTGATATTTT+AGG - chr6_3:57319450-57319469 Msa0954810:intron 25.0%
!!! GGTTTTTTCAACAAAAGAAA+AGG + chr6_3:57319581-57319600 None:intergenic 25.0%
! TGATCTTTATGTACTGTTTA+AGG + chr6_3:57319642-57319661 None:intergenic 25.0%
!!! TCTACCATGTTGATTTTTTT+TGG - chr6_3:57319844-57319863 Msa0954810:intron 25.0%
!!! CTACCATGTTGATTTTTTTT+GGG - chr6_3:57319845-57319864 Msa0954810:intron 25.0%
! AATAGAATATTGTTTCGATG+TGG - chr6_3:57319896-57319915 Msa0954810:intron 25.0%
! ATAGAATATTGTTTCGATGT+GGG - chr6_3:57319897-57319916 Msa0954810:intron 25.0%
! TCTCTCAATTAAAACATACA+GGG - chr6_3:57320205-57320224 Msa0954810:intron 25.0%
!! GAAACTTATTCTGAAAATGA+TGG - chr6_3:57320312-57320331 Msa0954810:intron 25.0%
! TTAACATTTAGTCCATCAAT+TGG + chr6_3:57320479-57320498 None:intergenic 25.0%
! CTCATTCATCTAAAAAACAA+GGG + chr6_3:57320646-57320665 None:intergenic 25.0%
!! ATACACTTGCATTTTACATA+GGG + chr6_3:57320680-57320699 None:intergenic 25.0%
!! TATACACTTGCATTTTACAT+AGG + chr6_3:57320681-57320700 None:intergenic 25.0%
! TCTAGTAATGTTACAATTTC+GGG - chr6_3:57320835-57320854 Msa0954810:five_prime_UTR 25.0%
!! TTAGGTTTGGAAAACATTTT+GGG + chr6_3:57318731-57318750 None:intergenic 25.0%
!!! TTTAGGTTTGGAAAACATTT+TGG + chr6_3:57318732-57318751 None:intergenic 25.0%
! GTTTATGAACAACACAAATT+GGG + chr6_3:57318758-57318777 None:intergenic 25.0%
!!! ATTTTGGGATTGATTTTGTT+AGG + chr6_3:57318780-57318799 None:intergenic 25.0%
!! AAGGGTTTAAGAACAATTTT+GGG + chr6_3:57318795-57318814 None:intergenic 25.0%
!! TAAGGGTTTAAGAACAATTT+TGG + chr6_3:57318796-57318815 None:intergenic 25.0%
! TGTTCAATTTATTCTCAAAC+TGG - chr6_3:57318942-57318961 Msa0954810:intron 25.0%
!! GAAAAGTTATGAGCTTTATA+TGG - chr6_3:57319209-57319228 Msa0954810:intron 25.0%
! TTTAATCAAACAAGATCCAA+TGG + chr6_3:57319357-57319376 None:intergenic 25.0%
!! TTGGATCTTGTTTGATTAAA+AGG - chr6_3:57319357-57319376 Msa0954810:intron 25.0%
!! TGGATCTTGTTTGATTAAAA+GGG - chr6_3:57319358-57319377 Msa0954810:intron 25.0%
!!! GGCATTTTGTTTGATATTTT+AGG - chr6_3:57319450-57319469 Msa0954810:intron 25.0%
!!! GGTTTTTTCAACAAAAGAAA+AGG + chr6_3:57319581-57319600 None:intergenic 25.0%
! TGATCTTTATGTACTGTTTA+AGG + chr6_3:57319642-57319661 None:intergenic 25.0%
!!! TCTACCATGTTGATTTTTTT+TGG - chr6_3:57319844-57319863 Msa0954810:intron 25.0%
!!! CTACCATGTTGATTTTTTTT+GGG - chr6_3:57319845-57319864 Msa0954810:intron 25.0%
! AATAGAATATTGTTTCGATG+TGG - chr6_3:57319896-57319915 Msa0954810:intron 25.0%
! ATAGAATATTGTTTCGATGT+GGG - chr6_3:57319897-57319916 Msa0954810:intron 25.0%
! TCTCTCAATTAAAACATACA+GGG - chr6_3:57320205-57320224 Msa0954810:intron 25.0%
!! GAAACTTATTCTGAAAATGA+TGG - chr6_3:57320312-57320331 Msa0954810:intron 25.0%
! TTAACATTTAGTCCATCAAT+TGG + chr6_3:57320479-57320498 None:intergenic 25.0%
! CTCATTCATCTAAAAAACAA+GGG + chr6_3:57320646-57320665 None:intergenic 25.0%
!! ATACACTTGCATTTTACATA+GGG + chr6_3:57320680-57320699 None:intergenic 25.0%
!! TATACACTTGCATTTTACAT+AGG + chr6_3:57320681-57320700 None:intergenic 25.0%
! TCTAGTAATGTTACAATTTC+GGG - chr6_3:57320835-57320854 Msa0954810:five_prime_UTR 25.0%
!!! CAAATTGGGAATTTTAGGTT+TGG + chr6_3:57318744-57318763 None:intergenic 30.0%
! CAACACAAATTGGGAATTTT+AGG + chr6_3:57318749-57318768 None:intergenic 30.0%
GGTTTATGAACAACACAAAT+TGG + chr6_3:57318759-57318778 None:intergenic 30.0%
AATCTAAACCATAAGAGAAG+AGG + chr6_3:57318993-57319012 None:intergenic 30.0%
! TACGGTTAGAAATAGCTTTA+GGG + chr6_3:57319298-57319317 None:intergenic 30.0%
TTACGGTTAGAAATAGCTTT+AGG + chr6_3:57319299-57319318 None:intergenic 30.0%
!! ATTTTGTTTGTGAATGGGTT+TGG - chr6_3:57319475-57319494 Msa0954810:intron 30.0%
!! AACAAGAAGTGTTAGGATTT+CGG - chr6_3:57319683-57319702 Msa0954810:intron 30.0%
ATAAAACCAAAAGCCATAAC+AGG + chr6_3:57319791-57319810 None:intergenic 30.0%
!!! GTTATGGCTTTTGGTTTTAT+AGG - chr6_3:57319791-57319810 Msa0954810:intron 30.0%
!! AGATTTGTTTGACTTGATCA+AGG + chr6_3:57319968-57319987 None:intergenic 30.0%
TGTAAAAGAGGTTTGAAGAT+AGG + chr6_3:57320033-57320052 None:intergenic 30.0%
!! CAACTTTCAAGTTTCAAGAT+AGG + chr6_3:57320060-57320079 None:intergenic 30.0%
CTCTCTCAATTAAAACATAC+AGG - chr6_3:57320204-57320223 Msa0954810:intron 30.0%
ATGATCCGGTAAATATTCAT+AGG - chr6_3:57320558-57320577 Msa0954810:intron 30.0%
CCTCATTCATCTAAAAAACA+AGG + chr6_3:57320647-57320666 None:intergenic 30.0%
!!! CCTTGTTTTTTAGATGAATG+AGG - chr6_3:57320644-57320663 Msa0954810:intron 30.0%
! TTTTAGATGAATGAGGCATA+AGG - chr6_3:57320651-57320670 Msa0954810:intron 30.0%
AAAATGTTCCTCAAGATCAA+TGG - chr6_3:57320973-57320992 Msa0954810:five_prime_UTR 30.0%
!!! CAAATTGGGAATTTTAGGTT+TGG + chr6_3:57318744-57318763 None:intergenic 30.0%
! CAACACAAATTGGGAATTTT+AGG + chr6_3:57318749-57318768 None:intergenic 30.0%
GGTTTATGAACAACACAAAT+TGG + chr6_3:57318759-57318778 None:intergenic 30.0%
AATCTAAACCATAAGAGAAG+AGG + chr6_3:57318993-57319012 None:intergenic 30.0%
! TACGGTTAGAAATAGCTTTA+GGG + chr6_3:57319298-57319317 None:intergenic 30.0%
TTACGGTTAGAAATAGCTTT+AGG + chr6_3:57319299-57319318 None:intergenic 30.0%
!! ATTTTGTTTGTGAATGGGTT+TGG - chr6_3:57319475-57319494 Msa0954810:intron 30.0%
!! AACAAGAAGTGTTAGGATTT+CGG - chr6_3:57319683-57319702 Msa0954810:intron 30.0%
ATAAAACCAAAAGCCATAAC+AGG + chr6_3:57319791-57319810 None:intergenic 30.0%
!!! GTTATGGCTTTTGGTTTTAT+AGG - chr6_3:57319791-57319810 Msa0954810:intron 30.0%
!! AGATTTGTTTGACTTGATCA+AGG + chr6_3:57319968-57319987 None:intergenic 30.0%
TGTAAAAGAGGTTTGAAGAT+AGG + chr6_3:57320033-57320052 None:intergenic 30.0%
!! CAACTTTCAAGTTTCAAGAT+AGG + chr6_3:57320060-57320079 None:intergenic 30.0%
CTCTCTCAATTAAAACATAC+AGG - chr6_3:57320204-57320223 Msa0954810:intron 30.0%
ATGATCCGGTAAATATTCAT+AGG - chr6_3:57320558-57320577 Msa0954810:intron 30.0%
CCTCATTCATCTAAAAAACA+AGG + chr6_3:57320647-57320666 None:intergenic 30.0%
!!! CCTTGTTTTTTAGATGAATG+AGG - chr6_3:57320644-57320663 Msa0954810:intron 30.0%
! TTTTAGATGAATGAGGCATA+AGG - chr6_3:57320651-57320670 Msa0954810:intron 30.0%
AAAATGTTCCTCAAGATCAA+TGG - chr6_3:57320973-57320992 Msa0954810:five_prime_UTR 30.0%
! AAATCGCGTTTGTGTGTTTT+GGG + chr6_3:57318613-57318632 None:intergenic 35.0%
TGAAGTGATTCTACGTTTCA+TGG + chr6_3:57318652-57318671 None:intergenic 35.0%
!! CAACAAAAGCATCGCAAATT+TGG + chr6_3:57318825-57318844 None:intergenic 35.0%
!! ACGGTTAGAAATAGCTTTAG+GGG + chr6_3:57319297-57319316 None:intergenic 35.0%
GAAATGGGGGGAAAATATTA+CGG + chr6_3:57319316-57319335 None:intergenic 35.0%
!! TAGGGCATTTTGTTTGTGAA+TGG - chr6_3:57319469-57319488 Msa0954810:intron 35.0%
!! AGGGCATTTTGTTTGTGAAT+GGG - chr6_3:57319470-57319489 Msa0954810:intron 35.0%
!! CACTTGAAACAAGAAGTGTT+AGG - chr6_3:57319676-57319695 Msa0954810:intron 35.0%
! TGTCCTATTTTCTGTTTAGG+TGG - chr6_3:57319755-57319774 Msa0954810:intron 35.0%
TGTCCACCTAAACAGAAAAT+AGG + chr6_3:57319761-57319780 None:intergenic 35.0%
! TATTTTCTGTTTAGGTGGAC+AGG - chr6_3:57319760-57319779 Msa0954810:intron 35.0%
CGAAACAATATTCTATTCCC+TGG + chr6_3:57319893-57319912 None:intergenic 35.0%
AAGATAGGCTCTTGTAAAAG+AGG + chr6_3:57320045-57320064 None:intergenic 35.0%
AAACTTAAGCTACTGCAAAG+TGG + chr6_3:57320103-57320122 None:intergenic 35.0%
!!! TTAGGTAGTGGACATTTTAG+TGG + chr6_3:57320378-57320397 None:intergenic 35.0%
!!! TCTTGTTTTCCTTGTAGACA+TGG - chr6_3:57320520-57320539 Msa0954810:intron 35.0%
TCGAAGATGTATGTATGATC+CGG - chr6_3:57320544-57320563 Msa0954810:intron 35.0%
! CATAGCCTTTTCTACAAACT+CGG + chr6_3:57320618-57320637 None:intergenic 35.0%
TGGCTATTAGGGTTCAATTT+CGG - chr6_3:57320931-57320950 Msa0954810:five_prime_UTR 35.0%
TTCGGGAAGTTTATGAGTAT+TGG - chr6_3:57320949-57320968 Msa0954810:five_prime_UTR 35.0%
TCGGGAAGTTTATGAGTATT+GGG - chr6_3:57320950-57320969 Msa0954810:five_prime_UTR 35.0%
TCAATGGTTTCCAACACTTT+CGG - chr6_3:57320989-57321008 Msa0954810:five_prime_UTR 35.0%
! TATATCCTTACCGAAAGTGT+TGG + chr6_3:57321002-57321021 None:intergenic 35.0%
! AAATCGCGTTTGTGTGTTTT+GGG + chr6_3:57318613-57318632 None:intergenic 35.0%
TGAAGTGATTCTACGTTTCA+TGG + chr6_3:57318652-57318671 None:intergenic 35.0%
!! CAACAAAAGCATCGCAAATT+TGG + chr6_3:57318825-57318844 None:intergenic 35.0%
!! ACGGTTAGAAATAGCTTTAG+GGG + chr6_3:57319297-57319316 None:intergenic 35.0%
GAAATGGGGGGAAAATATTA+CGG + chr6_3:57319316-57319335 None:intergenic 35.0%
!! TAGGGCATTTTGTTTGTGAA+TGG - chr6_3:57319469-57319488 Msa0954810:intron 35.0%
!! AGGGCATTTTGTTTGTGAAT+GGG - chr6_3:57319470-57319489 Msa0954810:intron 35.0%
!! CACTTGAAACAAGAAGTGTT+AGG - chr6_3:57319676-57319695 Msa0954810:intron 35.0%
! TGTCCTATTTTCTGTTTAGG+TGG - chr6_3:57319755-57319774 Msa0954810:intron 35.0%
TGTCCACCTAAACAGAAAAT+AGG + chr6_3:57319761-57319780 None:intergenic 35.0%
! TATTTTCTGTTTAGGTGGAC+AGG - chr6_3:57319760-57319779 Msa0954810:intron 35.0%
CGAAACAATATTCTATTCCC+TGG + chr6_3:57319893-57319912 None:intergenic 35.0%
AAGATAGGCTCTTGTAAAAG+AGG + chr6_3:57320045-57320064 None:intergenic 35.0%
AAACTTAAGCTACTGCAAAG+TGG + chr6_3:57320103-57320122 None:intergenic 35.0%
!!! TTAGGTAGTGGACATTTTAG+TGG + chr6_3:57320378-57320397 None:intergenic 35.0%
!!! TCTTGTTTTCCTTGTAGACA+TGG - chr6_3:57320520-57320539 Msa0954810:intron 35.0%
TCGAAGATGTATGTATGATC+CGG - chr6_3:57320544-57320563 Msa0954810:intron 35.0%
! CATAGCCTTTTCTACAAACT+CGG + chr6_3:57320618-57320637 None:intergenic 35.0%
TGGCTATTAGGGTTCAATTT+CGG - chr6_3:57320931-57320950 Msa0954810:five_prime_UTR 35.0%
TTCGGGAAGTTTATGAGTAT+TGG - chr6_3:57320949-57320968 Msa0954810:five_prime_UTR 35.0%
TCGGGAAGTTTATGAGTATT+GGG - chr6_3:57320950-57320969 Msa0954810:five_prime_UTR 35.0%
TCAATGGTTTCCAACACTTT+CGG - chr6_3:57320989-57321008 Msa0954810:five_prime_UTR 35.0%
! TATATCCTTACCGAAAGTGT+TGG + chr6_3:57321002-57321021 None:intergenic 35.0%
! GAAATCGCGTTTGTGTGTTT+TGG + chr6_3:57318614-57318633 None:intergenic 40.0%
TCTACGTTTCATGGTTTGAG+AGG + chr6_3:57318643-57318662 None:intergenic 40.0%
GGGAAGTGATTATGAGTGAT+GGG + chr6_3:57318711-57318730 None:intergenic 40.0%
TGGGAAGTGATTATGAGTGA+TGG + chr6_3:57318712-57318731 None:intergenic 40.0%
! CGCAAATTTGGAGCGATTAA+GGG + chr6_3:57318813-57318832 None:intergenic 40.0%
! TCGCAAATTTGGAGCGATTA+AGG + chr6_3:57318814-57318833 None:intergenic 40.0%
TCGAACAGAAAGTAGCTTGA+AGG + chr6_3:57318893-57318912 None:intergenic 40.0%
TCAACTGTCCTCTTCTCTTA+TGG - chr6_3:57318982-57319001 Msa0954810:intron 40.0%
TCAAACAAGATCCAATGGCT+CGG + chr6_3:57319352-57319371 None:intergenic 40.0%
! TTGTTTGTGAATGGGTTTGG+TGG - chr6_3:57319478-57319497 Msa0954810:intron 40.0%
ACAACTACACAATCTGGTCA+TGG - chr6_3:57319707-57319726 Msa0954810:intron 40.0%
CAACTACACAATCTGGTCAT+GGG - chr6_3:57319708-57319727 Msa0954810:intron 40.0%
AACTACACAATCTGGTCATG+GGG - chr6_3:57319709-57319728 Msa0954810:intron 40.0%
! GGCTGTCCTATTTTCTGTTT+AGG - chr6_3:57319752-57319771 Msa0954810:intron 40.0%
GCGCCCAAAAAAAATCAACA+TGG + chr6_3:57319851-57319870 None:intergenic 40.0%
ATATTGTTTCGATGTGGGAC+CGG - chr6_3:57319902-57319921 Msa0954810:intron 40.0%
CATCAATTGGCAGCATAAGA+TGG + chr6_3:57320466-57320485 None:intergenic 40.0%
CATCTTATGCTGCCAATTGA+TGG - chr6_3:57320464-57320483 Msa0954810:intron 40.0%
ATCTTCGATCCATGTCTACA+AGG + chr6_3:57320532-57320551 None:intergenic 40.0%
TCCGGTAAATATTCATAGGC+TGG - chr6_3:57320562-57320581 Msa0954810:intron 40.0%
CCCAGCCTATGAATATTTAC+CGG + chr6_3:57320566-57320585 None:intergenic 40.0%
CCGGTAAATATTCATAGGCT+GGG - chr6_3:57320563-57320582 Msa0954810:intron 40.0%
TTCTACAAACTCGGCTACTT+TGG + chr6_3:57320609-57320628 None:intergenic 40.0%
AGTAGCCGAGTTTGTAGAAA+AGG - chr6_3:57320610-57320629 Msa0954810:intron 40.0%
CGAGTTTGTAGAAAAGGCTA+TGG - chr6_3:57320616-57320635 Msa0954810:intron 40.0%
AGACTTAGTGAATCCTAGAG+AGG - chr6_3:57320700-57320719 Msa0954810:CDS 40.0%
! ATGCAATGAGGTTGGCTATT+AGG - chr6_3:57320919-57320938 Msa0954810:five_prime_UTR 40.0%
! TGCAATGAGGTTGGCTATTA+GGG - chr6_3:57320920-57320939 Msa0954810:five_prime_UTR 40.0%
GGCTATTAGGGTTCAATTTC+GGG - chr6_3:57320932-57320951 Msa0954810:five_prime_UTR 40.0%
GTTGGAAACCATTGATCTTG+AGG + chr6_3:57320984-57321003 None:intergenic 40.0%
! GAAATCGCGTTTGTGTGTTT+TGG + chr6_3:57318614-57318633 None:intergenic 40.0%
TCTACGTTTCATGGTTTGAG+AGG + chr6_3:57318643-57318662 None:intergenic 40.0%
GGGAAGTGATTATGAGTGAT+GGG + chr6_3:57318711-57318730 None:intergenic 40.0%
TGGGAAGTGATTATGAGTGA+TGG + chr6_3:57318712-57318731 None:intergenic 40.0%
! CGCAAATTTGGAGCGATTAA+GGG + chr6_3:57318813-57318832 None:intergenic 40.0%
! TCGCAAATTTGGAGCGATTA+AGG + chr6_3:57318814-57318833 None:intergenic 40.0%
TCGAACAGAAAGTAGCTTGA+AGG + chr6_3:57318893-57318912 None:intergenic 40.0%
TCAACTGTCCTCTTCTCTTA+TGG - chr6_3:57318982-57319001 Msa0954810:intron 40.0%
TCAAACAAGATCCAATGGCT+CGG + chr6_3:57319352-57319371 None:intergenic 40.0%
! TTGTTTGTGAATGGGTTTGG+TGG - chr6_3:57319478-57319497 Msa0954810:intron 40.0%
ACAACTACACAATCTGGTCA+TGG - chr6_3:57319707-57319726 Msa0954810:intron 40.0%
CAACTACACAATCTGGTCAT+GGG - chr6_3:57319708-57319727 Msa0954810:intron 40.0%
AACTACACAATCTGGTCATG+GGG - chr6_3:57319709-57319728 Msa0954810:intron 40.0%
! GGCTGTCCTATTTTCTGTTT+AGG - chr6_3:57319752-57319771 Msa0954810:intron 40.0%
GCGCCCAAAAAAAATCAACA+TGG + chr6_3:57319851-57319870 None:intergenic 40.0%
ATATTGTTTCGATGTGGGAC+CGG - chr6_3:57319902-57319921 Msa0954810:intron 40.0%
CATCAATTGGCAGCATAAGA+TGG + chr6_3:57320466-57320485 None:intergenic 40.0%
CATCTTATGCTGCCAATTGA+TGG - chr6_3:57320464-57320483 Msa0954810:intron 40.0%
ATCTTCGATCCATGTCTACA+AGG + chr6_3:57320532-57320551 None:intergenic 40.0%
TCCGGTAAATATTCATAGGC+TGG - chr6_3:57320562-57320581 Msa0954810:intron 40.0%
CCCAGCCTATGAATATTTAC+CGG + chr6_3:57320566-57320585 None:intergenic 40.0%
CCGGTAAATATTCATAGGCT+GGG - chr6_3:57320563-57320582 Msa0954810:intron 40.0%
TTCTACAAACTCGGCTACTT+TGG + chr6_3:57320609-57320628 None:intergenic 40.0%
AGTAGCCGAGTTTGTAGAAA+AGG - chr6_3:57320610-57320629 Msa0954810:intron 40.0%
CGAGTTTGTAGAAAAGGCTA+TGG - chr6_3:57320616-57320635 Msa0954810:intron 40.0%
AGACTTAGTGAATCCTAGAG+AGG - chr6_3:57320700-57320719 Msa0954810:CDS 40.0%
! ATGCAATGAGGTTGGCTATT+AGG - chr6_3:57320919-57320938 Msa0954810:five_prime_UTR 40.0%
! TGCAATGAGGTTGGCTATTA+GGG - chr6_3:57320920-57320939 Msa0954810:five_prime_UTR 40.0%
GGCTATTAGGGTTCAATTTC+GGG - chr6_3:57320932-57320951 Msa0954810:five_prime_UTR 40.0%
GTTGGAAACCATTGATCTTG+AGG + chr6_3:57320984-57321003 None:intergenic 40.0%
AAGTAGCTTGAAGGCAGCAA+CGG + chr6_3:57318884-57318903 None:intergenic 45.0%
AAAAGTGGAGGGAGAAATGG+GGG + chr6_3:57319329-57319348 None:intergenic 45.0%
GAAAAGTGGAGGGAGAAATG+GGG + chr6_3:57319330-57319349 None:intergenic 45.0%
GGAAAAGTGGAGGGAGAAAT+GGG + chr6_3:57319331-57319350 None:intergenic 45.0%
TTCGGCACAACTACACAATC+TGG - chr6_3:57319701-57319720 Msa0954810:intron 45.0%
TGGGCGCTTACTACAATTCA+CGG - chr6_3:57319864-57319883 Msa0954810:intron 45.0%
TACTACAATTCACGGTAGCC+AGG - chr6_3:57319872-57319891 Msa0954810:intron 45.0%
ACTACAATTCACGGTAGCCA+GGG - chr6_3:57319873-57319892 Msa0954810:intron 45.0%
TGGGTTCTCTAACTTTGACG+CGG + chr6_3:57319934-57319953 None:intergenic 45.0%
GATCAAGGAGAGAATGACCT+GGG + chr6_3:57319953-57319972 None:intergenic 45.0%
TGATCAAGGAGAGAATGACC+TGG + chr6_3:57319954-57319973 None:intergenic 45.0%
GGCAGCATAAGATGGCATAA+TGG + chr6_3:57320458-57320477 None:intergenic 45.0%
CTTAGTGAATCCTAGAGAGG+AGG - chr6_3:57320703-57320722 Msa0954810:CDS 45.0%
TCATCTTCATCCTCCTCTCT+AGG + chr6_3:57320716-57320735 None:intergenic 45.0%
AAGATGCTGCTCATGCAATG+AGG - chr6_3:57320907-57320926 Msa0954810:five_prime_UTR 45.0%
GGTTTCCAACACTTTCGGTA+AGG - chr6_3:57320994-57321013 Msa0954810:five_prime_UTR 45.0%
AAGTAGCTTGAAGGCAGCAA+CGG + chr6_3:57318884-57318903 None:intergenic 45.0%
AAAAGTGGAGGGAGAAATGG+GGG + chr6_3:57319329-57319348 None:intergenic 45.0%
GAAAAGTGGAGGGAGAAATG+GGG + chr6_3:57319330-57319349 None:intergenic 45.0%
GGAAAAGTGGAGGGAGAAAT+GGG + chr6_3:57319331-57319350 None:intergenic 45.0%
TTCGGCACAACTACACAATC+TGG - chr6_3:57319701-57319720 Msa0954810:intron 45.0%
TGGGCGCTTACTACAATTCA+CGG - chr6_3:57319864-57319883 Msa0954810:intron 45.0%
TACTACAATTCACGGTAGCC+AGG - chr6_3:57319872-57319891 Msa0954810:intron 45.0%
ACTACAATTCACGGTAGCCA+GGG - chr6_3:57319873-57319892 Msa0954810:intron 45.0%
TGGGTTCTCTAACTTTGACG+CGG + chr6_3:57319934-57319953 None:intergenic 45.0%
GATCAAGGAGAGAATGACCT+GGG + chr6_3:57319953-57319972 None:intergenic 45.0%
TGATCAAGGAGAGAATGACC+TGG + chr6_3:57319954-57319973 None:intergenic 45.0%
GGCAGCATAAGATGGCATAA+TGG + chr6_3:57320458-57320477 None:intergenic 45.0%
CTTAGTGAATCCTAGAGAGG+AGG - chr6_3:57320703-57320722 Msa0954810:CDS 45.0%
TCATCTTCATCCTCCTCTCT+AGG + chr6_3:57320716-57320735 None:intergenic 45.0%
AAGATGCTGCTCATGCAATG+AGG - chr6_3:57320907-57320926 Msa0954810:five_prime_UTR 45.0%
GGTTTCCAACACTTTCGGTA+AGG - chr6_3:57320994-57321013 Msa0954810:five_prime_UTR 45.0%
!!! AATATTTTCAAAATAAATTT+AGG + chr6_3:57320433-57320452 None:intergenic 5.0%
!!! AATATTTTCAAAATAAATTT+AGG + chr6_3:57320433-57320452 None:intergenic 5.0%
AAAGTGGAGGGAGAAATGGG+GGG + chr6_3:57319328-57319347 None:intergenic 50.0%
CGGAAAAGTGGAGGGAGAAA+TGG + chr6_3:57319332-57319351 None:intergenic 50.0%
! CAATGGCTCGGAAAAGTGGA+GGG + chr6_3:57319340-57319359 None:intergenic 50.0%
! GATCCAATGGCTCGGAAAAG+TGG + chr6_3:57319344-57319363 None:intergenic 50.0%
! GAGCAGCCTGTTATGGCTTT+TGG - chr6_3:57319782-57319801 Msa0954810:intron 50.0%
CTCTAACTTTGACGCGGGAA+AGG + chr6_3:57319928-57319947 None:intergenic 50.0%
GGGTTCTCTAACTTTGACGC+GGG + chr6_3:57319933-57319952 None:intergenic 50.0%
GCGTCAAAGTTAGAGAACCC+AGG - chr6_3:57319933-57319952 Msa0954810:intron 50.0%
TGCTGCTCATGCAATGAGGT+TGG - chr6_3:57320911-57320930 Msa0954810:five_prime_UTR 50.0%
AAAGTGGAGGGAGAAATGGG+GGG + chr6_3:57319328-57319347 None:intergenic 50.0%
CGGAAAAGTGGAGGGAGAAA+TGG + chr6_3:57319332-57319351 None:intergenic 50.0%
! CAATGGCTCGGAAAAGTGGA+GGG + chr6_3:57319340-57319359 None:intergenic 50.0%
! GATCCAATGGCTCGGAAAAG+TGG + chr6_3:57319344-57319363 None:intergenic 50.0%
! GAGCAGCCTGTTATGGCTTT+TGG - chr6_3:57319782-57319801 Msa0954810:intron 50.0%
CTCTAACTTTGACGCGGGAA+AGG + chr6_3:57319928-57319947 None:intergenic 50.0%
GGGTTCTCTAACTTTGACGC+GGG + chr6_3:57319933-57319952 None:intergenic 50.0%
GCGTCAAAGTTAGAGAACCC+AGG - chr6_3:57319933-57319952 Msa0954810:intron 50.0%
TGCTGCTCATGCAATGAGGT+TGG - chr6_3:57320911-57320930 Msa0954810:five_prime_UTR 50.0%
! CCAATGGCTCGGAAAAGTGG+AGG + chr6_3:57319341-57319360 None:intergenic 55.0%
!! CCTCCACTTTTCCGAGCCAT+TGG - chr6_3:57319338-57319357 Msa0954810:intron 55.0%
TGGACAGGAGCAGCCTGTTA+TGG - chr6_3:57319775-57319794 Msa0954810:intron 55.0%
AACTTTGACGCGGGAAAGGC+CGG + chr6_3:57319924-57319943 None:intergenic 55.0%
! CCAATGGCTCGGAAAAGTGG+AGG + chr6_3:57319341-57319360 None:intergenic 55.0%
!! CCTCCACTTTTCCGAGCCAT+TGG - chr6_3:57319338-57319357 Msa0954810:intron 55.0%
TGGACAGGAGCAGCCTGTTA+TGG - chr6_3:57319775-57319794 Msa0954810:intron 55.0%
AACTTTGACGCGGGAAAGGC+CGG + chr6_3:57319924-57319943 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 57318600 57321044 57318600 ID=Msa0954810;Name=Msa0954810
chr6_3 mRNA 57318600 57321044 57318600 ID=Msa0954810-mRNA-1;Parent=Msa0954810;Name=Msa0954810-mRNA-1;_AED=0.16;_eAED=0.16;_QI=233|0|1|1|1|1|2|151|43
chr6_3 exon 57318600 57318751 57318600 ID=Msa0954810-mRNA-1:exon:11494;Parent=Msa0954810-mRNA-1
chr6_3 exon 57320681 57321044 57320681 ID=Msa0954810-mRNA-1:exon:11493;Parent=Msa0954810-mRNA-1
chr6_3 five_prime_UTR 57320812 57321044 57320812 ID=Msa0954810-mRNA-1:five_prime_utr;Parent=Msa0954810-mRNA-1
chr6_3 CDS 57320681 57320811 57320681 ID=Msa0954810-mRNA-1:cds;Parent=Msa0954810-mRNA-1
chr6_3 CDS 57318751 57318751 57318751 ID=Msa0954810-mRNA-1:cds;Parent=Msa0954810-mRNA-1
chr6_3 three_prime_UTR 57318600 57318750 57318600 ID=Msa0954810-mRNA-1:three_prime_utr;Parent=Msa0954810-mRNA-1
Gene Sequence

>Msa0954810

ATGCTTTTGTTGTTTCTGTTGTTTTTGTACTCTTCATCTTCGATCTCGCCGTTGCTGCCTTCAAGCTACTTTCTGTTCGATTTCTCAAATCTGATGCAAAGCTTTGCTCTGTTCAATTTATTCTCAAACTGA
Protein sequence

>Msa0954810

MLLLFLLFLYSSSSISPLLPSSYFLFDFSNLMQSFALFNLFSN*