AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0887990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0887990 MtrunA17_Chr7g0271481 91.765 85 7 0 6 90 586 670 6.70e-51 171
Msa0887990 MtrunA17_Chr1g0210851 36.585 82 52 0 9 90 686 767 5.38e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0887990 AT3G07100.1 74.118 85 22 0 6 90 604 688 5.63e-41 143
Msa0887990 AT4G32640.2 34.146 82 54 0 9 90 669 750 5.75e-12 60.8
Msa0887990 AT4G32640.1 34.146 82 54 0 9 90 669 750 5.75e-12 60.8
Msa0887990 AT3G44340.5 35.366 82 53 0 9 90 672 753 5.92e-12 60.8
Msa0887990 AT3G44340.2 35.366 82 53 0 9 90 672 753 5.92e-12 60.8
Msa0887990 AT3G44340.1 35.366 82 53 0 9 90 672 753 5.93e-12 60.8
Msa0887990 AT3G44340.4 35.366 82 53 0 9 90 672 753 5.93e-12 60.8
Msa0887990 AT3G44340.3 35.366 82 53 0 9 90 672 753 5.93e-12 60.8

Find 18 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCATAAAATGGATCTTC+AGG 0.293277 6_1:-51653924 None:intergenic
AGAAATTGAGTCATGAATTA+AGG 0.308061 6_1:+51654450 Msa0887990:CDS
CATACACATTTACTGAGATT+TGG 0.310882 6_1:-51653971 None:intergenic
GCAGCCATCTGCTCATAAAA+TGG 0.311495 6_1:-51653933 None:intergenic
ATTTACTGAGATTTGGTACT+TGG 0.355044 6_1:-51653964 None:intergenic
TCTCCCCATGAGTTGCTGAT+TGG 0.417715 6_1:-51654430 None:intergenic
TTCTGTCTGTGTAGGAACTC+TGG 0.429146 6_1:+51654367 Msa0887990:intron
TGAGTTGCTGATTGGAAAGC+TGG 0.488951 6_1:-51654422 None:intergenic
GAGAGACTGCATGGGAAGCA+TGG 0.524555 6_1:+51654486 Msa0887990:CDS
CTTTCCAATCAGCAACTCAT+GGG 0.525222 6_1:+51654426 Msa0887990:CDS
GCTTTCCAATCAGCAACTCA+TGG 0.538623 6_1:+51654425 Msa0887990:CDS
ACCTCACAAGAGAGACTGCA+TGG 0.560035 6_1:+51654477 Msa0887990:CDS
GGAAAATAAAACACTTAAGG+AGG 0.565991 6_1:-51654021 None:intergenic
ACTCTGGCAAAGTACACTGC+TGG 0.591069 6_1:+51654383 Msa0887990:CDS
CCCATGCAGTCTCTCTTGTG+AGG 0.598716 6_1:-51654478 None:intergenic
TTTGGGACAGATAAAGAACA+TGG 0.635621 6_1:+51653891 Msa0887990:intron
TTTCCAATCAGCAACTCATG+GGG 0.670512 6_1:+51654427 Msa0887990:CDS
CCTCACAAGAGAGACTGCAT+GGG 0.693489 6_1:+51654478 Msa0887990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTGTATTAAGTTATATT+TGG + chr6_1:51653397-51653416 Msa0887990:intron 15.0%
!!! ATTTGGTAATTAGTTATATT+TGG + chr6_1:51653414-51653433 Msa0887990:intron 15.0%
!! TGTTTGTATTAAGTTATATT+TGG + chr6_1:51653397-51653416 Msa0887990:intron 15.0%
!!! ATTTGGTAATTAGTTATATT+TGG + chr6_1:51653414-51653433 Msa0887990:intron 15.0%
!! CGTAAATGTATATGTTTAAT+AGG + chr6_1:51653474-51653493 Msa0887990:intron 20.0%
!!! ATATTTTTGTTGTATTAGTG+TGG + chr6_1:51653682-51653701 Msa0887990:intron 20.0%
!! TAACAGGTTTAAAAATAGAA+TGG - chr6_1:51654072-51654091 None:intergenic 20.0%
!! CGTAAATGTATATGTTTAAT+AGG + chr6_1:51653474-51653493 Msa0887990:intron 20.0%
!!! ATATTTTTGTTGTATTAGTG+TGG + chr6_1:51653682-51653701 Msa0887990:intron 20.0%
!! TAACAGGTTTAAAAATAGAA+TGG - chr6_1:51654072-51654091 None:intergenic 20.0%
! TTGACTTTAATAAGATGCTT+TGG + chr6_1:51653361-51653380 Msa0887990:intron 25.0%
! AAAACATCCTGCAAATTAAT+AGG + chr6_1:51653437-51653456 Msa0887990:intron 25.0%
! ATTAATAGGATCACATGATT+AGG + chr6_1:51653451-51653470 Msa0887990:intron 25.0%
! ATGGTTTATATACAAAAAGG+AGG - chr6_1:51653548-51653567 None:intergenic 25.0%
! GTCATGGTTTATATACAAAA+AGG - chr6_1:51653551-51653570 None:intergenic 25.0%
!! TGTCTATCTATTTCTCAAAT+AGG - chr6_1:51653573-51653592 None:intergenic 25.0%
! AAAACTAGTCATTTGTTCAT+TGG + chr6_1:51653659-51653678 Msa0887990:intron 25.0%
! GTGATAAAATTGATATGCAA+TGG - chr6_1:51653737-51653756 None:intergenic 25.0%
! ATGTTCTGATATTTGTGTAA+TGG + chr6_1:51653783-51653802 Msa0887990:intron 25.0%
! AATTTCAACACACAAAAAGA+TGG - chr6_1:51653851-51653870 None:intergenic 25.0%
!!! AGTGTATTAGTGTATTGATT+TGG + chr6_1:51653873-51653892 Msa0887990:intron 25.0%
!!! GTGTATTAGTGTATTGATTT+GGG + chr6_1:51653874-51653893 Msa0887990:intron 25.0%
! GAAGGAAAATAAAACACTTA+AGG - chr6_1:51654027-51654046 None:intergenic 25.0%
! ACGAAATACATAGAAATTGA+AGG - chr6_1:51654045-51654064 None:intergenic 25.0%
! ATCAATTAATCAAGAGTAAC+AGG - chr6_1:51654088-51654107 None:intergenic 25.0%
! ACTTAATGACATGAGTTTAT+GGG + chr6_1:51654148-51654167 Msa0887990:intron 25.0%
! TAAACTGATACTTTAACTCA+TGG + chr6_1:51654191-51654210 Msa0887990:intron 25.0%
! AGAAATTGAGTCATGAATTA+AGG + chr6_1:51654450-51654469 Msa0887990:CDS 25.0%
! TTGACTTTAATAAGATGCTT+TGG + chr6_1:51653361-51653380 Msa0887990:intron 25.0%
! AAAACATCCTGCAAATTAAT+AGG + chr6_1:51653437-51653456 Msa0887990:intron 25.0%
! ATTAATAGGATCACATGATT+AGG + chr6_1:51653451-51653470 Msa0887990:intron 25.0%
! ATGGTTTATATACAAAAAGG+AGG - chr6_1:51653548-51653567 None:intergenic 25.0%
! GTCATGGTTTATATACAAAA+AGG - chr6_1:51653551-51653570 None:intergenic 25.0%
!! TGTCTATCTATTTCTCAAAT+AGG - chr6_1:51653573-51653592 None:intergenic 25.0%
! AAAACTAGTCATTTGTTCAT+TGG + chr6_1:51653659-51653678 Msa0887990:intron 25.0%
! GTGATAAAATTGATATGCAA+TGG - chr6_1:51653737-51653756 None:intergenic 25.0%
! ATGTTCTGATATTTGTGTAA+TGG + chr6_1:51653783-51653802 Msa0887990:intron 25.0%
! AATTTCAACACACAAAAAGA+TGG - chr6_1:51653851-51653870 None:intergenic 25.0%
!!! AGTGTATTAGTGTATTGATT+TGG + chr6_1:51653873-51653892 Msa0887990:intron 25.0%
!!! GTGTATTAGTGTATTGATTT+GGG + chr6_1:51653874-51653893 Msa0887990:intron 25.0%
! GAAGGAAAATAAAACACTTA+AGG - chr6_1:51654027-51654046 None:intergenic 25.0%
! ACGAAATACATAGAAATTGA+AGG - chr6_1:51654045-51654064 None:intergenic 25.0%
! ATCAATTAATCAAGAGTAAC+AGG - chr6_1:51654088-51654107 None:intergenic 25.0%
! ACTTAATGACATGAGTTTAT+GGG + chr6_1:51654148-51654167 Msa0887990:intron 25.0%
! TAAACTGATACTTTAACTCA+TGG + chr6_1:51654191-51654210 Msa0887990:intron 25.0%
! AGAAATTGAGTCATGAATTA+AGG + chr6_1:51654450-51654469 Msa0887990:CDS 25.0%
!! ATGTGATCCTATTAATTTGC+AGG - chr6_1:51653447-51653466 None:intergenic 30.0%
! TCTATTTCTCAAATAGGTCA+TGG - chr6_1:51653567-51653586 None:intergenic 30.0%
AAAATTGATATGCAATGGCA+AGG - chr6_1:51653732-51653751 None:intergenic 30.0%
!! GATATTTGTGTAATGGATAG+AGG + chr6_1:51653790-51653809 Msa0887990:intron 30.0%
! ATTTACTGAGATTTGGTACT+TGG - chr6_1:51653967-51653986 None:intergenic 30.0%
CATACACATTTACTGAGATT+TGG - chr6_1:51653974-51653993 None:intergenic 30.0%
GGAAAATAAAACACTTAAGG+AGG - chr6_1:51654024-51654043 None:intergenic 30.0%
! ATTTGATGATTCTTCAGTTG+TGG + chr6_1:51654110-51654129 Msa0887990:intron 30.0%
GACTTAATGACATGAGTTTA+TGG + chr6_1:51654147-51654166 Msa0887990:intron 30.0%
AAATGCTGACAGAATTACAT+CGG - chr6_1:51654282-51654301 None:intergenic 30.0%
!!! TGTTTTTTTTCTGTCTGTGT+AGG + chr6_1:51654359-51654378 Msa0887990:intron 30.0%
!! ATGTGATCCTATTAATTTGC+AGG - chr6_1:51653447-51653466 None:intergenic 30.0%
! TCTATTTCTCAAATAGGTCA+TGG - chr6_1:51653567-51653586 None:intergenic 30.0%
AAAATTGATATGCAATGGCA+AGG - chr6_1:51653732-51653751 None:intergenic 30.0%
!! GATATTTGTGTAATGGATAG+AGG + chr6_1:51653790-51653809 Msa0887990:intron 30.0%
! ATTTACTGAGATTTGGTACT+TGG - chr6_1:51653967-51653986 None:intergenic 30.0%
CATACACATTTACTGAGATT+TGG - chr6_1:51653974-51653993 None:intergenic 30.0%
GGAAAATAAAACACTTAAGG+AGG - chr6_1:51654024-51654043 None:intergenic 30.0%
! ATTTGATGATTCTTCAGTTG+TGG + chr6_1:51654110-51654129 Msa0887990:intron 30.0%
GACTTAATGACATGAGTTTA+TGG + chr6_1:51654147-51654166 Msa0887990:intron 30.0%
AAATGCTGACAGAATTACAT+CGG - chr6_1:51654282-51654301 None:intergenic 30.0%
!!! TGTTTTTTTTCTGTCTGTGT+AGG + chr6_1:51654359-51654378 Msa0887990:intron 30.0%
! ATAATTCTAGGAGCTAACAG+AGG + chr6_1:51653522-51653541 Msa0887990:intron 35.0%
! TTTGGGACAGATAAAGAACA+TGG + chr6_1:51653891-51653910 Msa0887990:intron 35.0%
TGCTCATAAAATGGATCTTC+AGG - chr6_1:51653927-51653946 None:intergenic 35.0%
! AGATCCATTTTATGAGCAGA+TGG + chr6_1:51653929-51653948 Msa0887990:CDS 35.0%
AATGCTGACAGAATTACATC+GGG - chr6_1:51654281-51654300 None:intergenic 35.0%
TGTAATTCTGTCAGCATTTG+CGG + chr6_1:51654283-51654302 Msa0887990:intron 35.0%
! ATAATTCTAGGAGCTAACAG+AGG + chr6_1:51653522-51653541 Msa0887990:intron 35.0%
! TTTGGGACAGATAAAGAACA+TGG + chr6_1:51653891-51653910 Msa0887990:intron 35.0%
TGCTCATAAAATGGATCTTC+AGG - chr6_1:51653927-51653946 None:intergenic 35.0%
! AGATCCATTTTATGAGCAGA+TGG + chr6_1:51653929-51653948 Msa0887990:CDS 35.0%
AATGCTGACAGAATTACATC+GGG - chr6_1:51654281-51654300 None:intergenic 35.0%
TGTAATTCTGTCAGCATTTG+CGG + chr6_1:51654283-51654302 Msa0887990:intron 35.0%
! ACTGTCAGAGGCATAATTCT+AGG + chr6_1:51653510-51653529 Msa0887990:intron 40.0%
!! TAATGGATAGAGGCATTGAG+TGG + chr6_1:51653800-51653819 Msa0887990:intron 40.0%
! CATTGAGTGGTGGAATTGAT+TGG + chr6_1:51653813-51653832 Msa0887990:intron 40.0%
CATCAGCACACAAGACTATA+TGG - chr6_1:51654317-51654336 None:intergenic 40.0%
CTTTCCAATCAGCAACTCAT+GGG + chr6_1:51654426-51654445 Msa0887990:CDS 40.0%
TTTCCAATCAGCAACTCATG+GGG + chr6_1:51654427-51654446 Msa0887990:CDS 40.0%
! ACTGTCAGAGGCATAATTCT+AGG + chr6_1:51653510-51653529 Msa0887990:intron 40.0%
!! TAATGGATAGAGGCATTGAG+TGG + chr6_1:51653800-51653819 Msa0887990:intron 40.0%
! CATTGAGTGGTGGAATTGAT+TGG + chr6_1:51653813-51653832 Msa0887990:intron 40.0%
CATCAGCACACAAGACTATA+TGG - chr6_1:51654317-51654336 None:intergenic 40.0%
CTTTCCAATCAGCAACTCAT+GGG + chr6_1:51654426-51654445 Msa0887990:CDS 40.0%
TTTCCAATCAGCAACTCATG+GGG + chr6_1:51654427-51654446 Msa0887990:CDS 40.0%
TCACATGTTGCAACTGTCAG+AGG + chr6_1:51653498-51653517 Msa0887990:intron 45.0%
GCAGCCATCTGCTCATAAAA+TGG - chr6_1:51653936-51653955 None:intergenic 45.0%
ACAGAATTACATCGGGACAC+GGG - chr6_1:51654274-51654293 None:intergenic 45.0%
GACAGAATTACATCGGGACA+CGG - chr6_1:51654275-51654294 None:intergenic 45.0%
TTCTGTCTGTGTAGGAACTC+TGG + chr6_1:51654367-51654386 Msa0887990:intron 45.0%
! TGAGTTGCTGATTGGAAAGC+TGG - chr6_1:51654425-51654444 None:intergenic 45.0%
GCTTTCCAATCAGCAACTCA+TGG + chr6_1:51654425-51654444 Msa0887990:CDS 45.0%
TCACATGTTGCAACTGTCAG+AGG + chr6_1:51653498-51653517 Msa0887990:intron 45.0%
GCAGCCATCTGCTCATAAAA+TGG - chr6_1:51653936-51653955 None:intergenic 45.0%
ACAGAATTACATCGGGACAC+GGG - chr6_1:51654274-51654293 None:intergenic 45.0%
GACAGAATTACATCGGGACA+CGG - chr6_1:51654275-51654294 None:intergenic 45.0%
TTCTGTCTGTGTAGGAACTC+TGG + chr6_1:51654367-51654386 Msa0887990:intron 45.0%
! TGAGTTGCTGATTGGAAAGC+TGG - chr6_1:51654425-51654444 None:intergenic 45.0%
GCTTTCCAATCAGCAACTCA+TGG + chr6_1:51654425-51654444 Msa0887990:CDS 45.0%
CAAGGCTTGTGCTCTGTCTT+GGG - chr6_1:51653714-51653733 None:intergenic 50.0%
!! TGGATAGAGGCATTGAGTGG+TGG + chr6_1:51653803-51653822 Msa0887990:intron 50.0%
CAGAATTACATCGGGACACG+GGG - chr6_1:51654273-51654292 None:intergenic 50.0%
ACTCTGGCAAAGTACACTGC+TGG + chr6_1:51654383-51654402 Msa0887990:CDS 50.0%
TCTCCCCATGAGTTGCTGAT+TGG - chr6_1:51654433-51654452 None:intergenic 50.0%
ACCTCACAAGAGAGACTGCA+TGG + chr6_1:51654477-51654496 Msa0887990:CDS 50.0%
CCTCACAAGAGAGACTGCAT+GGG + chr6_1:51654478-51654497 Msa0887990:CDS 50.0%
CAAGGCTTGTGCTCTGTCTT+GGG - chr6_1:51653714-51653733 None:intergenic 50.0%
!! TGGATAGAGGCATTGAGTGG+TGG + chr6_1:51653803-51653822 Msa0887990:intron 50.0%
CAGAATTACATCGGGACACG+GGG - chr6_1:51654273-51654292 None:intergenic 50.0%
ACTCTGGCAAAGTACACTGC+TGG + chr6_1:51654383-51654402 Msa0887990:CDS 50.0%
TCTCCCCATGAGTTGCTGAT+TGG - chr6_1:51654433-51654452 None:intergenic 50.0%
ACCTCACAAGAGAGACTGCA+TGG + chr6_1:51654477-51654496 Msa0887990:CDS 50.0%
CCTCACAAGAGAGACTGCAT+GGG + chr6_1:51654478-51654497 Msa0887990:CDS 50.0%
GCAAGGCTTGTGCTCTGTCT+TGG - chr6_1:51653715-51653734 None:intergenic 55.0%
CCCATGCAGTCTCTCTTGTG+AGG - chr6_1:51654481-51654500 None:intergenic 55.0%
GAGAGACTGCATGGGAAGCA+TGG + chr6_1:51654486-51654505 Msa0887990:CDS 55.0%
GCAAGGCTTGTGCTCTGTCT+TGG - chr6_1:51653715-51653734 None:intergenic 55.0%
CCCATGCAGTCTCTCTTGTG+AGG - chr6_1:51654481-51654500 None:intergenic 55.0%
GAGAGACTGCATGGGAAGCA+TGG + chr6_1:51654486-51654505 Msa0887990:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 51653325 51654541 51653325 ID=Msa0887990;Name=Msa0887990
chr6_1 mRNA 51653325 51654541 51653325 ID=Msa0887990-mRNA-1;Parent=Msa0887990;Name=Msa0887990-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|0.5|0|0.25|4|0|101
chr6_1 exon 51653325 51653333 51653325 ID=Msa0887990-mRNA-1:exon:10437;Parent=Msa0887990-mRNA-1
chr6_1 exon 51653875 51653881 51653875 ID=Msa0887990-mRNA-1:exon:10438;Parent=Msa0887990-mRNA-1
chr6_1 exon 51653901 51654029 51653901 ID=Msa0887990-mRNA-1:exon:10439;Parent=Msa0887990-mRNA-1
chr6_1 exon 51654381 51654541 51654381 ID=Msa0887990-mRNA-1:exon:10440;Parent=Msa0887990-mRNA-1
chr6_1 CDS 51653325 51653333 51653325 ID=Msa0887990-mRNA-1:cds;Parent=Msa0887990-mRNA-1
chr6_1 CDS 51653875 51653881 51653875 ID=Msa0887990-mRNA-1:cds;Parent=Msa0887990-mRNA-1
chr6_1 CDS 51653901 51654029 51653901 ID=Msa0887990-mRNA-1:cds;Parent=Msa0887990-mRNA-1
chr6_1 CDS 51654381 51654541 51654381 ID=Msa0887990-mRNA-1:cds;Parent=Msa0887990-mRNA-1
Gene Sequence

>Msa0887990

ATGTGTGATTGTATTAATAAAGAACATGGACTGAGACTGCCTGAAGATCCATTTTATGAGCAGATGGCTGCTGAGTTTTCCAAGTACCAAATCTCAGTAAATGTGTATGCATTCAGTGATAAGTACACTGACATAGCCTCCTTAAGAACTCTGGCAAAGTACACTGCTGGTCAAGTGTATTACTTTCCAGCTTTCCAATCAGCAACTCATGGGGAGAAATTGAGTCATGAATTAAGGAGATACCTCACAAGAGAGACTGCATGGGAAGCATGGATCATATGCATTGCAGAGATACTTAGTAACTAG

Protein sequence

>Msa0887990

MCDCINKEHGLRLPEDPFYEQMAAEFSKYQISVNVYAFSDKYTDIASLRTLAKYTAGQVYYFPAFQSATHGEKLSHELRRYLTRETAWEAWIICIAEILSN*