AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1397610


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1397610 MtrunA17_Chr7g0271481 92.941 85 6 0 6 90 586 670 3.30e-52 175
Msa1397610 MtrunA17_Chr1g0210851 36.585 82 52 0 9 90 686 767 4.56e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1397610 AT3G07100.1 75.294 85 21 0 6 90 604 688 3.94e-42 146
Msa1397610 AT4G32640.2 34.146 82 54 0 9 90 669 750 4.88e-12 60.8
Msa1397610 AT4G32640.1 34.146 82 54 0 9 90 669 750 4.88e-12 60.8
Msa1397610 AT3G44340.5 35.366 82 53 0 9 90 672 753 5.02e-12 60.8
Msa1397610 AT3G44340.2 35.366 82 53 0 9 90 672 753 5.02e-12 60.8
Msa1397610 AT3G44340.1 35.366 82 53 0 9 90 672 753 5.02e-12 60.8
Msa1397610 AT3G44340.4 35.366 82 53 0 9 90 672 753 5.02e-12 60.8
Msa1397610 AT3G44340.3 35.366 82 53 0 9 90 672 753 5.02e-12 60.8

Find 19 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCATAAAATGGATCTTC+AGG 0.293277 tig0022168:+43914 None:intergenic
AGAAATTGAGTCATGAATTA+AGG 0.308061 tig0022168:-43387 Msa1397610:CDS
CATACACATTTACTGAGATT+TGG 0.310882 tig0022168:+43867 None:intergenic
GCAGCCATCTGCTCATAAAA+TGG 0.311495 tig0022168:+43905 None:intergenic
ATTTACTGAGATTTGGTACT+TGG 0.355044 tig0022168:+43874 None:intergenic
TCCTGTCTGTGTAGGAACTC+TGG 0.429146 tig0022168:-43470 Msa1397610:intron
TCTCCCCATGAGTAGCTGAT+TGG 0.442822 tig0022168:+43407 None:intergenic
TGAGTAGCTGATTGGAAAGC+TGG 0.469035 tig0022168:+43415 None:intergenic
GAGAGACTGCATGGGAAGCA+TGG 0.524555 tig0022168:-43351 Msa1397610:CDS
GCTTTCCAATCAGCTACTCA+TGG 0.528786 tig0022168:-43412 Msa1397610:CDS
TACACTGACATAGCCTCCTT+AGG 0.536987 tig0022168:-43830 Msa1397610:intron
CTTTCCAATCAGCTACTCAT+GGG 0.542155 tig0022168:-43411 Msa1397610:CDS
GGAAAATAAAACACCTAAGG+AGG 0.554451 tig0022168:+43817 None:intergenic
ACCTCACAAGAGAGACTGCA+TGG 0.560035 tig0022168:-43360 Msa1397610:CDS
ACTCTGGCAAAGTACACTGC+TGG 0.591069 tig0022168:-43454 Msa1397610:CDS
CCCATGCAGTCTCTCTTGTG+AGG 0.598716 tig0022168:+43359 None:intergenic
TTTGGGACAGATAAAGAACA+TGG 0.635621 tig0022168:-43947 Msa1397610:intron
TTTCCAATCAGCTACTCATG+GGG 0.686768 tig0022168:-43410 Msa1397610:CDS
CCTCACAAGAGAGACTGCAT+GGG 0.693489 tig0022168:-43359 Msa1397610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTGTATTAAGTTATATT+TGG - tig0022168:43390-43409 Msa1397610:CDS 15.0%
!!! ATTTGGTAATTAGTTATATT+TGG - tig0022168:43407-43426 Msa1397610:CDS 15.0%
!! TGTTTGTATTAAGTTATATT+TGG - tig0022168:43390-43409 Msa1397610:CDS 15.0%
!!! ATTTGGTAATTAGTTATATT+TGG - tig0022168:43407-43426 Msa1397610:CDS 15.0%
!! CGTAAATGTATATGTTTAAT+AGG - tig0022168:43506-43525 Msa1397610:intron 20.0%
!!! ATATTTTTGTTGTATTAGTG+TGG - tig0022168:43714-43733 Msa1397610:intron 20.0%
!! TAACAGGTTTAAAAATAGAA+TGG + tig0022168:44104-44123 None:intergenic 20.0%
!! CGTAAATGTATATGTTTAAT+AGG - tig0022168:43506-43525 Msa1397610:intron 20.0%
!!! ATATTTTTGTTGTATTAGTG+TGG - tig0022168:43714-43733 Msa1397610:intron 20.0%
!! TAACAGGTTTAAAAATAGAA+TGG + tig0022168:44104-44123 None:intergenic 20.0%
! TTGACTTTAATAAGATGCTT+TGG - tig0022168:43354-43373 Msa1397610:CDS 25.0%
! ATGGTTTATATACAAAAAGG+AGG + tig0022168:43580-43599 None:intergenic 25.0%
! GTCATGGTTTATATACAAAA+AGG + tig0022168:43583-43602 None:intergenic 25.0%
!! TGTCTATCTATTTCTCAAAT+AGG + tig0022168:43605-43624 None:intergenic 25.0%
! AAAACTAGTCATTTGTTCAT+TGG - tig0022168:43691-43710 Msa1397610:intron 25.0%
! GTGATAAAATTGATATGCAA+TGG + tig0022168:43769-43788 None:intergenic 25.0%
! ATGTTCTGATATTTGTGTAA+TGG - tig0022168:43815-43834 Msa1397610:intron 25.0%
! AATTTCAACACACAAAAAGA+TGG + tig0022168:43883-43902 None:intergenic 25.0%
!!! AGTGTATTAGTGTATTGATT+TGG - tig0022168:43905-43924 Msa1397610:CDS 25.0%
!!! GTGTATTAGTGTATTGATTT+GGG - tig0022168:43906-43925 Msa1397610:CDS 25.0%
! ACGAAATACATAGAAATTGA+AGG + tig0022168:44077-44096 None:intergenic 25.0%
! ATCAATTAATCAAGAGTAAC+AGG + tig0022168:44120-44139 None:intergenic 25.0%
!! ATTTGATGATTCTTTAGTTG+TGG - tig0022168:44142-44161 Msa1397610:intron 25.0%
! ACTTAATGACATGAGTTTAT+GGG - tig0022168:44180-44199 Msa1397610:intron 25.0%
! TAAACTGATACTTTAACTCA+TGG - tig0022168:44223-44242 Msa1397610:intron 25.0%
! AGAAATTGAGTCATGAATTA+AGG - tig0022168:44483-44502 Msa1397610:intron 25.0%
! TTGACTTTAATAAGATGCTT+TGG - tig0022168:43354-43373 Msa1397610:CDS 25.0%
! ATGGTTTATATACAAAAAGG+AGG + tig0022168:43580-43599 None:intergenic 25.0%
! GTCATGGTTTATATACAAAA+AGG + tig0022168:43583-43602 None:intergenic 25.0%
!! TGTCTATCTATTTCTCAAAT+AGG + tig0022168:43605-43624 None:intergenic 25.0%
! AAAACTAGTCATTTGTTCAT+TGG - tig0022168:43691-43710 Msa1397610:intron 25.0%
! GTGATAAAATTGATATGCAA+TGG + tig0022168:43769-43788 None:intergenic 25.0%
! ATGTTCTGATATTTGTGTAA+TGG - tig0022168:43815-43834 Msa1397610:intron 25.0%
! AATTTCAACACACAAAAAGA+TGG + tig0022168:43883-43902 None:intergenic 25.0%
!!! AGTGTATTAGTGTATTGATT+TGG - tig0022168:43905-43924 Msa1397610:CDS 25.0%
!!! GTGTATTAGTGTATTGATTT+GGG - tig0022168:43906-43925 Msa1397610:CDS 25.0%
! ACGAAATACATAGAAATTGA+AGG + tig0022168:44077-44096 None:intergenic 25.0%
! ATCAATTAATCAAGAGTAAC+AGG + tig0022168:44120-44139 None:intergenic 25.0%
!! ATTTGATGATTCTTTAGTTG+TGG - tig0022168:44142-44161 Msa1397610:intron 25.0%
! ACTTAATGACATGAGTTTAT+GGG - tig0022168:44180-44199 Msa1397610:intron 25.0%
! TAAACTGATACTTTAACTCA+TGG - tig0022168:44223-44242 Msa1397610:intron 25.0%
! AGAAATTGAGTCATGAATTA+AGG - tig0022168:44483-44502 Msa1397610:intron 25.0%
!! TGATCTGCATATTAATTTGC+AGG + tig0022168:43440-43459 None:intergenic 30.0%
TGCAAATTAATATGCAGATC+AGG - tig0022168:43439-43458 Msa1397610:CDS 30.0%
ACTTAGTGCTGTATATGATT+AGG - tig0022168:43483-43502 Msa1397610:intron 30.0%
! TCTATTTCTCAAATAGGTCA+TGG + tig0022168:43599-43618 None:intergenic 30.0%
AAAATTGATATGCAATGGCA+AGG + tig0022168:43764-43783 None:intergenic 30.0%
!! GATATTTGTGTAATGGATAG+AGG - tig0022168:43822-43841 Msa1397610:intron 30.0%
! ATTTACTGAGATTTGGTACT+TGG + tig0022168:43999-44018 None:intergenic 30.0%
CATACACATTTACTGAGATT+TGG + tig0022168:44006-44025 None:intergenic 30.0%
GAAGGAAAATAAAACACCTA+AGG + tig0022168:44059-44078 None:intergenic 30.0%
GACTTAATGACATGAGTTTA+TGG - tig0022168:44179-44198 Msa1397610:intron 30.0%
!! TGATCTGCATATTAATTTGC+AGG + tig0022168:43440-43459 None:intergenic 30.0%
TGCAAATTAATATGCAGATC+AGG - tig0022168:43439-43458 Msa1397610:CDS 30.0%
ACTTAGTGCTGTATATGATT+AGG - tig0022168:43483-43502 Msa1397610:intron 30.0%
! TCTATTTCTCAAATAGGTCA+TGG + tig0022168:43599-43618 None:intergenic 30.0%
AAAATTGATATGCAATGGCA+AGG + tig0022168:43764-43783 None:intergenic 30.0%
!! GATATTTGTGTAATGGATAG+AGG - tig0022168:43822-43841 Msa1397610:intron 30.0%
! ATTTACTGAGATTTGGTACT+TGG + tig0022168:43999-44018 None:intergenic 30.0%
CATACACATTTACTGAGATT+TGG + tig0022168:44006-44025 None:intergenic 30.0%
GAAGGAAAATAAAACACCTA+AGG + tig0022168:44059-44078 None:intergenic 30.0%
GACTTAATGACATGAGTTTA+TGG - tig0022168:44179-44198 Msa1397610:intron 30.0%
! ATAATTCTAGGAGCTAACAG+AGG - tig0022168:43554-43573 Msa1397610:intron 35.0%
! TTTGGGACAGATAAAGAACA+TGG - tig0022168:43923-43942 Msa1397610:CDS 35.0%
TGCTCATAAAATGGATCTTC+AGG + tig0022168:43959-43978 None:intergenic 35.0%
! AGATCCATTTTATGAGCAGA+TGG - tig0022168:43961-43980 Msa1397610:intron 35.0%
GGAAAATAAAACACCTAAGG+AGG + tig0022168:44056-44075 None:intergenic 35.0%
AAATGCTGACAGAATTACAC+CGG + tig0022168:44314-44333 None:intergenic 35.0%
TGTAATTCTGTCAGCATTTG+CGG - tig0022168:44315-44334 Msa1397610:intron 35.0%
!!! GTTTTTTTTCCTGTCTGTGT+AGG - tig0022168:44392-44411 Msa1397610:intron 35.0%
! ATAATTCTAGGAGCTAACAG+AGG - tig0022168:43554-43573 Msa1397610:intron 35.0%
! TTTGGGACAGATAAAGAACA+TGG - tig0022168:43923-43942 Msa1397610:CDS 35.0%
TGCTCATAAAATGGATCTTC+AGG + tig0022168:43959-43978 None:intergenic 35.0%
! AGATCCATTTTATGAGCAGA+TGG - tig0022168:43961-43980 Msa1397610:intron 35.0%
GGAAAATAAAACACCTAAGG+AGG + tig0022168:44056-44075 None:intergenic 35.0%
AAATGCTGACAGAATTACAC+CGG + tig0022168:44314-44333 None:intergenic 35.0%
TGTAATTCTGTCAGCATTTG+CGG - tig0022168:44315-44334 Msa1397610:intron 35.0%
!!! GTTTTTTTTCCTGTCTGTGT+AGG - tig0022168:44392-44411 Msa1397610:intron 35.0%
! ACTGTCAGAGGCATAATTCT+AGG - tig0022168:43542-43561 Msa1397610:intron 40.0%
!! TAATGGATAGAGGCATTGAG+TGG - tig0022168:43832-43851 Msa1397610:CDS 40.0%
AATGCTGACAGAATTACACC+GGG + tig0022168:44313-44332 None:intergenic 40.0%
CATCAGCACACAAGACTATA+TGG + tig0022168:44349-44368 None:intergenic 40.0%
CTTTCCAATCAGCTACTCAT+GGG - tig0022168:44459-44478 Msa1397610:intron 40.0%
TTTCCAATCAGCTACTCATG+GGG - tig0022168:44460-44479 Msa1397610:intron 40.0%
! ACTGTCAGAGGCATAATTCT+AGG - tig0022168:43542-43561 Msa1397610:intron 40.0%
!! TAATGGATAGAGGCATTGAG+TGG - tig0022168:43832-43851 Msa1397610:CDS 40.0%
AATGCTGACAGAATTACACC+GGG + tig0022168:44313-44332 None:intergenic 40.0%
CATCAGCACACAAGACTATA+TGG + tig0022168:44349-44368 None:intergenic 40.0%
CTTTCCAATCAGCTACTCAT+GGG - tig0022168:44459-44478 Msa1397610:intron 40.0%
TTTCCAATCAGCTACTCATG+GGG - tig0022168:44460-44479 Msa1397610:intron 40.0%
TCACATGTTGCAACTGTCAG+AGG - tig0022168:43530-43549 Msa1397610:intron 45.0%
! CATTGAGTGGTGGAATTGGT+TGG - tig0022168:43845-43864 Msa1397610:CDS 45.0%
GCAGCCATCTGCTCATAAAA+TGG + tig0022168:43968-43987 None:intergenic 45.0%
TACACTGACATAGCCTCCTT+AGG - tig0022168:44040-44059 Msa1397610:intron 45.0%
ATCAATGTATCCCTGTGTCC+CGG - tig0022168:44292-44311 Msa1397610:intron 45.0%
TGAGTAGCTGATTGGAAAGC+TGG + tig0022168:44458-44477 None:intergenic 45.0%
GCTTTCCAATCAGCTACTCA+TGG - tig0022168:44458-44477 Msa1397610:intron 45.0%
TCACATGTTGCAACTGTCAG+AGG - tig0022168:43530-43549 Msa1397610:intron 45.0%
! CATTGAGTGGTGGAATTGGT+TGG - tig0022168:43845-43864 Msa1397610:CDS 45.0%
GCAGCCATCTGCTCATAAAA+TGG + tig0022168:43968-43987 None:intergenic 45.0%
TACACTGACATAGCCTCCTT+AGG - tig0022168:44040-44059 Msa1397610:intron 45.0%
ATCAATGTATCCCTGTGTCC+CGG - tig0022168:44292-44311 Msa1397610:intron 45.0%
TGAGTAGCTGATTGGAAAGC+TGG + tig0022168:44458-44477 None:intergenic 45.0%
GCTTTCCAATCAGCTACTCA+TGG - tig0022168:44458-44477 Msa1397610:intron 45.0%
CAAGGCTTGTGCTCTGTCTT+GGG + tig0022168:43746-43765 None:intergenic 50.0%
!! TGGATAGAGGCATTGAGTGG+TGG - tig0022168:43835-43854 Msa1397610:CDS 50.0%
!! GAGGCATTGAGTGGTGGAAT+TGG - tig0022168:43841-43860 Msa1397610:CDS 50.0%
CAGAATTACACCGGGACACA+GGG + tig0022168:44305-44324 None:intergenic 50.0%
ACAGAATTACACCGGGACAC+AGG + tig0022168:44306-44325 None:intergenic 50.0%
TCCTGTCTGTGTAGGAACTC+TGG - tig0022168:44400-44419 Msa1397610:intron 50.0%
ACTCTGGCAAAGTACACTGC+TGG - tig0022168:44416-44435 Msa1397610:intron 50.0%
TCTCCCCATGAGTAGCTGAT+TGG + tig0022168:44466-44485 None:intergenic 50.0%
ACCTCACAAGAGAGACTGCA+TGG - tig0022168:44510-44529 Msa1397610:intron 50.0%
CCTCACAAGAGAGACTGCAT+GGG - tig0022168:44511-44530 Msa1397610:intron 50.0%
CAAGGCTTGTGCTCTGTCTT+GGG + tig0022168:43746-43765 None:intergenic 50.0%
!! TGGATAGAGGCATTGAGTGG+TGG - tig0022168:43835-43854 Msa1397610:CDS 50.0%
!! GAGGCATTGAGTGGTGGAAT+TGG - tig0022168:43841-43860 Msa1397610:CDS 50.0%
CAGAATTACACCGGGACACA+GGG + tig0022168:44305-44324 None:intergenic 50.0%
ACAGAATTACACCGGGACAC+AGG + tig0022168:44306-44325 None:intergenic 50.0%
TCCTGTCTGTGTAGGAACTC+TGG - tig0022168:44400-44419 Msa1397610:intron 50.0%
ACTCTGGCAAAGTACACTGC+TGG - tig0022168:44416-44435 Msa1397610:intron 50.0%
TCTCCCCATGAGTAGCTGAT+TGG + tig0022168:44466-44485 None:intergenic 50.0%
ACCTCACAAGAGAGACTGCA+TGG - tig0022168:44510-44529 Msa1397610:intron 50.0%
CCTCACAAGAGAGACTGCAT+GGG - tig0022168:44511-44530 Msa1397610:intron 50.0%
GCAAGGCTTGTGCTCTGTCT+TGG + tig0022168:43747-43766 None:intergenic 55.0%
GCCAGAGTTCCTACACAGAC+AGG + tig0022168:44404-44423 None:intergenic 55.0%
CCCATGCAGTCTCTCTTGTG+AGG + tig0022168:44514-44533 None:intergenic 55.0%
GAGAGACTGCATGGGAAGCA+TGG - tig0022168:44519-44538 Msa1397610:intron 55.0%
GCAAGGCTTGTGCTCTGTCT+TGG + tig0022168:43747-43766 None:intergenic 55.0%
GCCAGAGTTCCTACACAGAC+AGG + tig0022168:44404-44423 None:intergenic 55.0%
CCCATGCAGTCTCTCTTGTG+AGG + tig0022168:44514-44533 None:intergenic 55.0%
GAGAGACTGCATGGGAAGCA+TGG - tig0022168:44519-44538 Msa1397610:intron 55.0%
Chromosome Type Strat End Strand Name
tig0022168 gene 43318 44574 43318 ID=Msa1397610;Name=Msa1397610
tig0022168 mRNA 43318 44574 43318 ID=Msa1397610-mRNA-1;Parent=Msa1397610;Name=Msa1397610-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|0.5|1|1|4|0|101
tig0022168 exon 44566 44574 44566 ID=Msa1397610-mRNA-1:exon:16192;Parent=Msa1397610-mRNA-1
tig0022168 exon 43979 43985 43979 ID=Msa1397610-mRNA-1:exon:16191;Parent=Msa1397610-mRNA-1
tig0022168 exon 43831 43959 43831 ID=Msa1397610-mRNA-1:exon:16190;Parent=Msa1397610-mRNA-1
tig0022168 exon 43318 43478 43318 ID=Msa1397610-mRNA-1:exon:16189;Parent=Msa1397610-mRNA-1
tig0022168 CDS 44566 44574 44566 ID=Msa1397610-mRNA-1:cds;Parent=Msa1397610-mRNA-1
tig0022168 CDS 43979 43985 43979 ID=Msa1397610-mRNA-1:cds;Parent=Msa1397610-mRNA-1
tig0022168 CDS 43831 43959 43831 ID=Msa1397610-mRNA-1:cds;Parent=Msa1397610-mRNA-1
tig0022168 CDS 43318 43478 43318 ID=Msa1397610-mRNA-1:cds;Parent=Msa1397610-mRNA-1
Gene Sequence

>Msa1397610

ATGTGTGATTGTATTAATAAAGAACATGGACTGAGACTGCCTGAAGATCCATTTTATGAGCAGATGGCTGCTGAGTTTTCCAAGTACCAAATCTCAGTAAATGTGTATGCATTCAGTGATAAGTACACTGACATAGCCTCCTTAGGAACTCTGGCAAAGTACACTGCTGGTCAAGTGTATTACTTTCCAGCTTTCCAATCAGCTACTCATGGGGAGAAATTGAGTCATGAATTAAGGAGATACCTCACAAGAGAGACTGCATGGGAAGCATGGATCATATGCATTGCAGAGATACTTAGTAACTAG

Protein sequence

>Msa1397610

MCDCINKEHGLRLPEDPFYEQMAAEFSKYQISVNVYAFSDKYTDIASLGTLAKYTAGQVYYFPAFQSATHGEKLSHELRRYLTRETAWEAWIICIAEILSN*