Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0667270 | Msa0958070 | 0.805004 | 1.645339e-49 | -8.615850e-47 |
| Msa0419670 | Msa0958070 | 0.803042 | 4.210043e-49 | -8.615850e-47 |
| Msa0589280 | Msa0958070 | 0.806062 | 9.869985e-50 | -8.615850e-47 |
| Msa0958070 | Msa0958080 | 0.838476 | 2.811005e-57 | -8.615850e-47 |
| Msa0958070 | Msa0972770 | 0.805790 | 1.125957e-49 | -8.615850e-47 |
| Msa0958070 | Msa0992640 | 0.833538 | 5.036152e-56 | -8.615850e-47 |
| Msa0958070 | Msa0992680 | 0.836323 | 1.001038e-56 | -8.615850e-47 |
| Msa0958070 | Msa1428070 | 0.844867 | 5.794000e-59 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACAGCCTGTCAGGATATT+AGG | 0.166266 | 6_3:-70799461 | Msa0958070:CDS |
| ATAAGGTCATACCATGTTTC+TGG | 0.242419 | 6_3:+70799887 | None:intergenic |
| TACAGCCTGTCAGGATATTA+GGG | 0.380743 | 6_3:-70799460 | Msa0958070:CDS |
| GATATGAAGGCACTAGTGTT+TGG | 0.462342 | 6_3:+70799921 | None:intergenic |
| CAATTTCGACCTACTGCCTA+TGG | 0.468037 | 6_3:-70799988 | Msa0958070:CDS |
| ACCACCTCATACAGCCTGTC+AGG | 0.489353 | 6_3:-70799469 | Msa0958070:CDS |
| ATATCCTGACAGGCTGTATG+AGG | 0.527822 | 6_3:+70799465 | None:intergenic |
| GGTATCGCTGTTCGAGGCAA+AGG | 0.529890 | 6_3:+70799489 | None:intergenic |
| TGAGGTGGTATCGCTGTTCG+AGG | 0.570695 | 6_3:+70799483 | None:intergenic |
| CATACAGAACATGGTAGACA+AGG | 0.572772 | 6_3:-70800054 | Msa0958070:CDS |
| GGATACGAGATATGATATGA+AGG | 0.590270 | 6_3:+70799908 | None:intergenic |
| TGAGAGGTGGCATAGAGAGA+TGG | 0.607888 | 6_3:-70800012 | Msa0958070:CDS |
| CTAGACCCTAATATCCTGAC+AGG | 0.613935 | 6_3:+70799455 | None:intergenic |
| TCCTGACAGGCTGTATGAGG+TGG | 0.623252 | 6_3:+70799468 | None:intergenic |
| TATGGAGATGAAGATCATGC+TGG | 0.631156 | 6_3:-70799970 | Msa0958070:CDS |
| TCTTCATCTCCATAGGCAGT+AGG | 0.649230 | 6_3:+70799979 | None:intergenic |
| TGATGTATGAGTTTGTTGAG+AGG | 0.654164 | 6_3:-70800028 | Msa0958070:CDS |
| TGTATGAGTTTGTTGAGAGG+TGG | 0.664087 | 6_3:-70800025 | Msa0958070:CDS |
| ATATCTCGTATCCAGAAACA+TGG | 0.665228 | 6_3:-70799898 | Msa0958070:intron |
| AGCATGATCTTCATCTCCAT+AGG | 0.667799 | 6_3:+70799972 | None:intergenic |
| TCACGAGTACATACAGAACA+TGG | 0.676915 | 6_3:-70800063 | Msa0958070:CDS |
| ATACAGAACATGGTAGACAA+GGG | 0.707864 | 6_3:-70800053 | Msa0958070:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTTTTTGCATATCACATA+AGG | + | chr6_3:70799980-70799999 | None:intergenic | 25.0% |
| TGCACTCAAATCACAATAAA+TGG | + | chr6_3:70799811-70799830 | None:intergenic | 30.0% | |
| CATACCTAAAACAACTAGTA+AGG | + | chr6_3:70799865-70799884 | None:intergenic | 30.0% | |
| !! | TTTTAGGTATGCATTTGTTG+TGG | - | chr6_3:70799874-70799893 | Msa0958070:intron | 30.0% |
| TATGCAAAAAAGTTCCTTGA+TGG | - | chr6_3:70799987-70800006 | Msa0958070:CDS | 30.0% | |
| ATACAGAACATGGTAGACAA+GGG | - | chr6_3:70799476-70799495 | Msa0958070:CDS | 35.0% | |
| !! | TGATGTATGAGTTTGTTGAG+AGG | - | chr6_3:70799501-70799520 | Msa0958070:CDS | 35.0% |
| GGATACGAGATATGATATGA+AGG | + | chr6_3:70799624-70799643 | None:intergenic | 35.0% | |
| ATATCTCGTATCCAGAAACA+TGG | - | chr6_3:70799631-70799650 | Msa0958070:intron | 35.0% | |
| ATAAGGTCATACCATGTTTC+TGG | + | chr6_3:70799645-70799664 | None:intergenic | 35.0% | |
| GTTGACATGTTTGCTTTAGT+TGG | - | chr6_3:70799724-70799743 | Msa0958070:intron | 35.0% | |
| TGTGAACGTTGAACTCTATT+TGG | - | chr6_3:70799836-70799855 | Msa0958070:intron | 35.0% | |
| !! | GTGTCCTTACTAGTTGTTTT+AGG | - | chr6_3:70799858-70799877 | Msa0958070:intron | 35.0% |
| TCACGAGTACATACAGAACA+TGG | - | chr6_3:70799466-70799485 | Msa0958070:CDS | 40.0% | |
| CATACAGAACATGGTAGACA+AGG | - | chr6_3:70799475-70799494 | Msa0958070:CDS | 40.0% | |
| !! | TGTATGAGTTTGTTGAGAGG+TGG | - | chr6_3:70799504-70799523 | Msa0958070:intron | 40.0% |
| AGCATGATCTTCATCTCCAT+AGG | + | chr6_3:70799560-70799579 | None:intergenic | 40.0% | |
| TATGGAGATGAAGATCATGC+TGG | - | chr6_3:70799559-70799578 | Msa0958070:intron | 40.0% | |
| !! | GATATGAAGGCACTAGTGTT+TGG | + | chr6_3:70799611-70799630 | None:intergenic | 40.0% |
| TGACCTTATCTGATAACCAC+AGG | - | chr6_3:70799656-70799675 | Msa0958070:intron | 40.0% | |
| GACCTTATCTGATAACCACA+GGG | - | chr6_3:70799657-70799676 | Msa0958070:intron | 40.0% | |
| ACCTTATCTGATAACCACAG+GGG | - | chr6_3:70799658-70799677 | Msa0958070:intron | 40.0% | |
| ATTGAAATCGAAGCCACAAG+AGG | - | chr6_3:70799702-70799721 | Msa0958070:intron | 40.0% | |
| CTTGTACATGTATCTCAGTG+TGG | - | chr6_3:70799909-70799928 | Msa0958070:CDS | 40.0% | |
| AATTATCATCCACACTCCAC+AGG | + | chr6_3:70799946-70799965 | None:intergenic | 40.0% | |
| TATCACATAAGGTCCTCTAG+CGG | + | chr6_3:70799969-70799988 | None:intergenic | 40.0% | |
| AGCAAGTGATCTATCCATCA+AGG | + | chr6_3:70800004-70800023 | None:intergenic | 40.0% | |
| ATACAGCCTGTCAGGATATT+AGG | - | chr6_3:70800068-70800087 | Msa0958070:CDS | 40.0% | |
| TACAGCCTGTCAGGATATTA+GGG | - | chr6_3:70800069-70800088 | Msa0958070:CDS | 40.0% | |
| CAATTTCGACCTACTGCCTA+TGG | - | chr6_3:70799541-70799560 | Msa0958070:intron | 45.0% | |
| TCTTCATCTCCATAGGCAGT+AGG | + | chr6_3:70799553-70799572 | None:intergenic | 45.0% | |
| ACCCCTGTGGTTATCAGATA+AGG | + | chr6_3:70799662-70799681 | None:intergenic | 45.0% | |
| GCAAACATGTCAACCTCTTG+TGG | + | chr6_3:70799718-70799737 | None:intergenic | 45.0% | |
| ! | GCATTTTTACTGCACGTTGC+AGG | - | chr6_3:70799780-70799799 | Msa0958070:intron | 45.0% |
| GTGGATGATAATTCCGCTAG+AGG | - | chr6_3:70799953-70799972 | Msa0958070:CDS | 45.0% | |
| GATAGATCACTTGCTTCCAC+AGG | - | chr6_3:70800009-70800028 | Msa0958070:CDS | 45.0% | |
| ATATCCTGACAGGCTGTATG+AGG | + | chr6_3:70800067-70800086 | None:intergenic | 45.0% | |
| CTAGACCCTAATATCCTGAC+AGG | + | chr6_3:70800077-70800096 | None:intergenic | 45.0% | |
| TGAGAGGTGGCATAGAGAGA+TGG | - | chr6_3:70799517-70799536 | Msa0958070:intron | 50.0% | |
| !! | TGTACGAGTTTTGACCCCTG+TGG | + | chr6_3:70799675-70799694 | None:intergenic | 50.0% |
| ACAGGGGTCAAAACTCGTAC+AGG | - | chr6_3:70799674-70799693 | Msa0958070:intron | 50.0% | |
| TTGGTTGCGTGATGAGCATC+TGG | - | chr6_3:70799743-70799762 | Msa0958070:intron | 50.0% | |
| TGTGGCATGTGCAAATCCTG+TGG | - | chr6_3:70799927-70799946 | Msa0958070:CDS | 50.0% | |
| TGTGCAAATCCTGTGGAGTG+TGG | - | chr6_3:70799934-70799953 | Msa0958070:CDS | 50.0% | |
| ! | GGCAAAGGTGTGAAATCCTG+TGG | + | chr6_3:70800028-70800047 | None:intergenic | 50.0% |
| GGTATCGCTGTTCGAGGCAA+AGG | + | chr6_3:70800043-70800062 | None:intergenic | 55.0% | |
| TGAGGTGGTATCGCTGTTCG+AGG | + | chr6_3:70800049-70800068 | None:intergenic | 55.0% | |
| ACCACCTCATACAGCCTGTC+AGG | - | chr6_3:70800060-70800079 | Msa0958070:CDS | 55.0% | |
| TCCTGACAGGCTGTATGAGG+TGG | + | chr6_3:70800064-70800083 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 70799455 | 70800096 | 70799455 | ID=Msa0958070;Name=Msa0958070 |
| chr6_3 | mRNA | 70799455 | 70800096 | 70799455 | ID=Msa0958070-mRNA-1;Parent=Msa0958070;Name=Msa0958070-mRNA-1;_AED=0.61;_eAED=1.00;_QI=0|0|0|0.5|1|1|2|0|87 |
| chr6_3 | exon | 70799899 | 70800096 | 70799899 | ID=Msa0958070-mRNA-1:exon:12962;Parent=Msa0958070-mRNA-1 |
| chr6_3 | exon | 70799455 | 70799520 | 70799455 | ID=Msa0958070-mRNA-1:exon:12961;Parent=Msa0958070-mRNA-1 |
| chr6_3 | CDS | 70799899 | 70800096 | 70799899 | ID=Msa0958070-mRNA-1:cds;Parent=Msa0958070-mRNA-1 |
| chr6_3 | CDS | 70799455 | 70799520 | 70799455 | ID=Msa0958070-mRNA-1:cds;Parent=Msa0958070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
