AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0963930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 72 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAGTAATTATGGAATATT+TGG 0.180018 6_3:-89488037 Msa0963930:five_prime_UTR
TGTGGTTAGCTTTGACTTTA+GGG 0.184467 6_3:-89488443 Msa0963930:five_prime_UTR
ATGTTGCAAAAGTATTTATT+AGG 0.216534 6_3:-89488284 Msa0963930:five_prime_UTR
TTGTGGTTAGCTTTGACTTT+AGG 0.227557 6_3:-89488444 Msa0963930:five_prime_UTR
ATCTTGCCCCAAACAGCTTA+TGG 0.274736 6_3:+89488131 None:intergenic
TATCATGATGGAACAATTAT+AGG 0.277634 6_3:+89488620 None:intergenic
CTCCTGATCATCACAGTAAA+AGG 0.283646 6_3:+89488059 None:intergenic
TGGATAATTCTGCTGGAAAA+AGG 0.285711 6_3:-89487931 Msa0963930:five_prime_UTR
TTTCCCTTCCTCTTCCTTGC+TGG 0.288095 6_3:-89488556 Msa0963930:five_prime_UTR
CTAGAAGCCATAAGCTGTTT+GGG 0.310222 6_3:-89488138 Msa0963930:five_prime_UTR
TGATCAGGAGTGAGTAATTA+TGG 0.311768 6_3:-89488046 Msa0963930:five_prime_UTR
AAATTGTTGCTGTGCTGTTT+GGG 0.331540 6_3:-89488169 Msa0963930:five_prime_UTR
AAGGAGGTAGAACTCAAGTT+AGG 0.334230 6_3:-89488406 Msa0963930:five_prime_UTR
TAAGTTACAGTTAAGCTGTT+TGG 0.340215 6_3:-89487770 Msa0963930:CDS
GGAAAGCTTTGGCAAGGATT+TGG 0.346050 6_3:+89488641 None:intergenic
GAGTACATCATGAGCTGAAT+TGG 0.347185 6_3:-89487732 Msa0963930:CDS
TGTTGCAAAAGTATTTATTA+GGG 0.356311 6_3:-89488283 Msa0963930:five_prime_UTR
ATTATGTATATTGGACTGTT+AGG 0.359865 6_3:-89488200 Msa0963930:five_prime_UTR
AACAGCTTAACTGTAACTTA+TGG 0.364660 6_3:+89487773 None:intergenic
CCGAATTCACTCCTTCCTCT+TGG 0.366416 6_3:-89488533 Msa0963930:five_prime_UTR
AGGTATAAGAAGTGATTAAT+TGG 0.370125 6_3:-89488352 Msa0963930:five_prime_UTR
GCTAGAAGCCATAAGCTGTT+TGG 0.379587 6_3:-89488139 Msa0963930:five_prime_UTR
GAAATTGTTGCTGTGCTGTT+TGG 0.379789 6_3:-89488170 Msa0963930:five_prime_UTR
TTTCCGAGTTCATTACTAAT+CGG 0.402134 6_3:+89487967 None:intergenic
GCATGCTTATGTTGCTGAAA+TGG 0.402854 6_3:-89488467 Msa0963930:five_prime_UTR
GACTTTAGGGACTGTATTTG+AGG 0.403112 6_3:-89488430 Msa0963930:five_prime_UTR
TGTTTGGAGAAAAGTGCTTA+TGG 0.405597 6_3:-89487754 Msa0963930:CDS
GAAATTTATATCCGATTTGA+AGG 0.410271 6_3:+89487998 None:intergenic
GATAATTGATTAATTTGAAG+TGG 0.420224 6_3:-89488601 Msa0963930:five_prime_UTR
ATCAAAACTCTTATAATCAT+AGG 0.424497 6_3:+89487829 None:intergenic
CCAAGAGGAAGGAGTGAATT+CGG 0.440352 6_3:+89488533 None:intergenic
GAACAATTATAGGAAAGCTT+TGG 0.442551 6_3:+89488630 None:intergenic
CTGTGAATGATTATGTATAT+TGG 0.443198 6_3:-89488209 Msa0963930:five_prime_UTR
TTGAAGTTGGATAATTCTGC+TGG 0.447928 6_3:-89487938 Msa0963930:five_prime_UTR
AAGTTTAATTCCCTTCAAAT+CGG 0.449463 6_3:-89488009 Msa0963930:five_prime_UTR
TGATACTTTGCCTGCTACTA+CGG 0.452591 6_3:-89487662 Msa0963930:CDS
AAATTATTATGATTGTTGTT+CGG 0.453250 6_3:-89488105 Msa0963930:five_prime_UTR
TCGGAAAGTGCGTTTGAAGT+TGG 0.455733 6_3:-89487951 Msa0963930:five_prime_UTR
AAGTTCATCTGTGTCACTCT+TGG 0.456761 6_3:-89486227 Msa0963930:CDS
GAGAGAGATTGGACAAGGGA+GGG 0.457922 6_3:+89488672 None:intergenic
TTCATCTGTGTCACTCTTGG+TGG 0.458234 6_3:-89486224 Msa0963930:CDS
ATTATGATTGTTGTTCGGAC+AGG 0.463586 6_3:-89488100 Msa0963930:five_prime_UTR
TTGAGAAATGAGCTAAATTG+TGG 0.464665 6_3:-89487886 Msa0963930:five_prime_UTR
AAATTAATCAATTATCATGA+TGG 0.465157 6_3:+89488608 None:intergenic
TGGATTTAGACTAGTTAGAA+AGG 0.467465 6_3:-89487866 Msa0963930:five_prime_UTR
AGAGAGATTGGACAAGGGAG+GGG 0.473116 6_3:+89488673 None:intergenic
TCTGTGTCACTCTTGGTGGT+AGG 0.473983 6_3:-89486220 None:intergenic
CTCGAGCAGCGTGGATAACT+CGG 0.479794 6_3:-89487707 Msa0963930:CDS
TGAGAGAGATTGGACAAGGG+AGG 0.484694 6_3:+89488671 None:intergenic
AAGTATCAACTATCCAATCT+TGG 0.488066 6_3:+89487677 None:intergenic
AGTACATCATGAGCTGAATT+GGG 0.494607 6_3:-89487731 Msa0963930:CDS
CGTGAGATAGAGAGAAAGAA+AGG 0.495618 6_3:+89488700 None:intergenic
ATAATTGATTAATTTGAAGT+GGG 0.496711 6_3:-89488600 Msa0963930:five_prime_UTR
GGACTGTATTTGAGGAAAGG+AGG 0.500005 6_3:-89488422 Msa0963930:five_prime_UTR
AGGAGGTAGAACTCAAGTTA+GGG 0.514168 6_3:-89488405 Msa0963930:five_prime_UTR
CCAACTGCTTCCGTAGTAGC+AGG 0.517506 6_3:+89487652 None:intergenic
GAATTCGGCCAGCAAGGAAG+AGG 0.534414 6_3:+89488548 None:intergenic
TAGGGACTGTATTTGAGGAA+AGG 0.536169 6_3:-89488425 Msa0963930:five_prime_UTR
TTATGTTGCTGAAATGGTTG+TGG 0.542434 6_3:-89488461 Msa0963930:five_prime_UTR
TGTTGCTGTGCTGTTTGGGA+TGG 0.543910 6_3:-89488165 Msa0963930:five_prime_UTR
ATTATAGGAAAGCTTTGGCA+AGG 0.546118 6_3:+89488635 None:intergenic
AGGAGTGAATTCGGCCAGCA+AGG 0.558853 6_3:+89488542 None:intergenic
CAACTTGGAATTGAAGATGT+AGG 0.565947 6_3:-89488375 Msa0963930:five_prime_UTR
CCTGCTACTACGGAAGCAGT+TGG 0.573503 6_3:-89487652 Msa0963930:CDS
AAATTTATATCCGATTTGAA+GGG 0.575743 6_3:+89487999 None:intergenic
TCGGCCAGCAAGGAAGAGGA+AGG 0.597065 6_3:+89488552 None:intergenic
ATTCCGATTAGTAATGAACT+CGG 0.629078 6_3:-89487970 Msa0963930:five_prime_UTR
AAGCAGTTGGAGAGAAAATG+TGG 0.630940 6_3:-89487639 Msa0963930:CDS
CTTGGAATTGAAGATGTAGG+AGG 0.638210 6_3:-89488372 Msa0963930:five_prime_UTR
TAGAAGCCATAAGCTGTTTG+GGG 0.639981 6_3:-89488137 Msa0963930:five_prime_UTR
CGGCCAGCAAGGAAGAGGAA+GGG 0.652342 6_3:+89488553 None:intergenic
GAATTGGGACTCGAGCAGCG+TGG 0.739005 6_3:-89487716 Msa0963930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATTATTATTATTAT+TGG - chr6_3:89487377-89487396 Msa0963930:intron 0.0%
!! TAATAATAATTAAAATAAGC+GGG + chr6_3:89487367-89487386 None:intergenic 10.0%
!!! ATATAAAACATTTTCAATTA+GGG + chr6_3:89487959-89487978 None:intergenic 10.0%
!!! AATATAAAACATTTTCAATT+AGG + chr6_3:89487960-89487979 None:intergenic 10.0%
!! AAATTAATCAATTATCATGA+TGG + chr6_3:89486339-89486358 None:intergenic 15.0%
!!! ATAATTGATTAATTTGAAGT+GGG - chr6_3:89486344-89486363 Msa0963930:intron 15.0%
!!! TTTTTTTTTTTGAGATTTGA+GGG - chr6_3:89486451-89486470 Msa0963930:intron 15.0%
!!! AAATTATTATGATTGTTGTT+CGG - chr6_3:89486839-89486858 Msa0963930:intron 15.0%
!! TATGTCATTATTTACTAAAA+TGG - chr6_3:89487428-89487447 Msa0963930:intron 15.0%
!! ATGTCATTATTTACTAAAAT+GGG - chr6_3:89487429-89487448 Msa0963930:intron 15.0%
!! TTTAGTGAAAATAATCAAAT+GGG + chr6_3:89488093-89488112 None:intergenic 15.0%
!! ATTTAGTGAAAATAATCAAA+TGG + chr6_3:89488094-89488113 None:intergenic 15.0%
!! TTAGTGAAAATAAACAAAAT+GGG + chr6_3:89488254-89488273 None:intergenic 15.0%
!! TTTAGTGAAAATAAACAAAA+TGG + chr6_3:89488255-89488274 None:intergenic 15.0%
!!! GTTTTCTATATTTTCTTTAA+AGG - chr6_3:89488279-89488298 Msa0963930:five_prime_UTR 15.0%
!!! AGAAAAATCTCATTTTGTTT+GGG + chr6_3:89486224-89486243 None:intergenic 20.0%
!!! GATAATTGATTAATTTGAAG+TGG - chr6_3:89486343-89486362 Msa0963930:intron 20.0%
!!! GTTTTTTTTTTTGAGATTTG+AGG - chr6_3:89486450-89486469 Msa0963930:intron 20.0%
!!! ATGTTGCAAAAGTATTTATT+AGG - chr6_3:89486660-89486679 Msa0963930:intron 20.0%
!!! TGTTGCAAAAGTATTTATTA+GGG - chr6_3:89486661-89486680 Msa0963930:intron 20.0%
!!! AAATTTATATCCGATTTGAA+GGG + chr6_3:89486948-89486967 None:intergenic 20.0%
!! ATCAAAACTCTTATAATCAT+AGG + chr6_3:89487118-89487137 None:intergenic 20.0%
!! TGTCATTATTTACTAAAATG+GGG - chr6_3:89487430-89487449 Msa0963930:intron 20.0%
!!! TTGGATTTGAATTTGAATTT+TGG - chr6_3:89487521-89487540 Msa0963930:intron 20.0%
!! ATAAAGTAGAGAAATTGAAA+CGG - chr6_3:89487560-89487579 Msa0963930:intron 20.0%
!!! GAATGATGTTTTGAAAATTA+CGG - chr6_3:89487806-89487825 Msa0963930:CDS 20.0%
!!! AGGTTATTTATCAAGATTTT+CGG - chr6_3:89488299-89488318 Msa0963930:five_prime_UTR 20.0%
!!! TTTCGGCAATATATTTTTAA+AGG - chr6_3:89488351-89488370 Msa0963930:five_prime_UTR 20.0%
!!! AAGGCTTTTATTTTGATTTT+CGG - chr6_3:89488370-89488389 Msa0963930:five_prime_UTR 20.0%
!!! TTTCGGCAATTTATTTTAAA+AGG - chr6_3:89488387-89488406 Msa0963930:five_prime_UTR 20.0%
!! ATATATACTTCCAATCAAAT+AGG + chr6_3:89488466-89488485 None:intergenic 20.0%
!!! CAGATTATTAGAATTTTAGA+CGG - chr6_3:89488518-89488537 Msa0963930:five_prime_UTR 20.0%
!! GAGAAAAATCTCATTTTGTT+TGG + chr6_3:89486225-89486244 None:intergenic 25.0%
!! TATCATGATGGAACAATTAT+AGG + chr6_3:89486327-89486346 None:intergenic 25.0%
! AACATTCAAAAAAATCCAAG+AGG + chr6_3:89486429-89486448 None:intergenic 25.0%
!!! TCTTGGATTTTTTTGAATGT+TGG - chr6_3:89486428-89486447 Msa0963930:intron 25.0%
! AGGTATAAGAAGTGATTAAT+TGG - chr6_3:89486592-89486611 Msa0963930:intron 25.0%
! CTGTGAATGATTATGTATAT+TGG - chr6_3:89486735-89486754 Msa0963930:intron 25.0%
! ATTATGTATATTGGACTGTT+AGG - chr6_3:89486744-89486763 Msa0963930:intron 25.0%
! GTGAGTAATTATGGAATATT+TGG - chr6_3:89486907-89486926 Msa0963930:intron 25.0%
! AAGTTTAATTCCCTTCAAAT+CGG - chr6_3:89486935-89486954 Msa0963930:intron 25.0%
!! GAAATTTATATCCGATTTGA+AGG + chr6_3:89486949-89486968 None:intergenic 25.0%
!!! TTTGAAATATGCGTTTTGAA+AGG - chr6_3:89487637-89487656 Msa0963930:CDS 25.0%
! TTTCGTTATGTTGTGAAATA+TGG - chr6_3:89487764-89487783 Msa0963930:CDS 25.0%
! TTGAAGTATACCTATTTGAT+TGG - chr6_3:89488453-89488472 Msa0963930:five_prime_UTR 25.0%
!! TTGGAAGTATATATAATGTG+TGG - chr6_3:89488472-89488491 Msa0963930:five_prime_UTR 25.0%
GAACAATTATAGGAAAGCTT+TGG + chr6_3:89486317-89486336 None:intergenic 30.0%
TTCAAAAAAATCCAAGAGGA+AGG + chr6_3:89486425-89486444 None:intergenic 30.0%
ATTCCGATTAGTAATGAACT+CGG - chr6_3:89486974-89486993 Msa0963930:intron 30.0%
TTTCCGAGTTCATTACTAAT+CGG + chr6_3:89486980-89486999 None:intergenic 30.0%
TTGAGAAATGAGCTAAATTG+TGG - chr6_3:89487058-89487077 Msa0963930:intron 30.0%
! TGGATTTAGACTAGTTAGAA+AGG - chr6_3:89487078-89487097 Msa0963930:intron 30.0%
!! AAGAGTTTTGATCGAAGTTT+TGG - chr6_3:89487126-89487145 Msa0963930:intron 30.0%
!!! ATCGAAGTTTTGGATGAATT+CGG - chr6_3:89487136-89487155 Msa0963930:intron 30.0%
AACAGCTTAACTGTAACTTA+TGG + chr6_3:89487174-89487193 None:intergenic 30.0%
TAAGTTACAGTTAAGCTGTT+TGG - chr6_3:89487174-89487193 Msa0963930:intron 30.0%
AAGTATCAACTATCCAATCT+TGG + chr6_3:89487270-89487289 None:intergenic 30.0%
ATGACGAGGAAGATATTTAA+AGG + chr6_3:89487464-89487483 None:intergenic 30.0%
GTCATCGTTATATAAGTTCA+TGG - chr6_3:89487479-89487498 Msa0963930:intron 30.0%
CGTTATATAAGTTCATGGAT+TGG - chr6_3:89487484-89487503 Msa0963930:intron 30.0%
!! ATAAGTTCATGGATTGGATT+TGG - chr6_3:89487490-89487509 Msa0963930:intron 30.0%
! TTTTGATATGTTGTGAAACG+TGG - chr6_3:89487613-89487632 Msa0963930:intron 30.0%
!! ATCTTAATTTGTACGCGATT+TGG - chr6_3:89487664-89487683 Msa0963930:CDS 30.0%
ACCTAGTAGTGATTTCAATT+AGG - chr6_3:89487853-89487872 Msa0963930:five_prime_UTR 30.0%
ACCTAATTGAAATCACTACT+AGG + chr6_3:89487857-89487876 None:intergenic 30.0%
! TTTTCGGCAATATCTTTTAG+AGG - chr6_3:89488315-89488334 Msa0963930:five_prime_UTR 30.0%
! GAGGCTCTTATTATGATTTT+CGG - chr6_3:89488334-89488353 Msa0963930:five_prime_UTR 30.0%
TCTTCATGTCTTTACAATGT+TGG - chr6_3:89488422-89488441 Msa0963930:five_prime_UTR 30.0%
!! TTTTGAGAGAGATTGGACAA+GGG + chr6_3:89486279-89486298 None:intergenic 35.0%
!! ATTTTGAGAGAGATTGGACA+AGG + chr6_3:89486280-89486299 None:intergenic 35.0%
!! TGGTGAATTTTGAGAGAGAT+TGG + chr6_3:89486286-89486305 None:intergenic 35.0%
! ATTATAGGAAAGCTTTGGCA+AGG + chr6_3:89486312-89486331 None:intergenic 35.0%
TTATGTTGCTGAAATGGTTG+TGG - chr6_3:89486483-89486502 Msa0963930:intron 35.0%
!! TTGTGGTTAGCTTTGACTTT+AGG - chr6_3:89486500-89486519 Msa0963930:intron 35.0%
!! TGTGGTTAGCTTTGACTTTA+GGG - chr6_3:89486501-89486520 Msa0963930:intron 35.0%
CAACTTGGAATTGAAGATGT+AGG - chr6_3:89486569-89486588 Msa0963930:intron 35.0%
!! AAATTGTTGCTGTGCTGTTT+GGG - chr6_3:89486775-89486794 Msa0963930:intron 35.0%
! ATTATGATTGTTGTTCGGAC+AGG - chr6_3:89486844-89486863 Msa0963930:intron 35.0%
! TACCTTTTACTGTGATGATC+AGG - chr6_3:89486883-89486902 Msa0963930:intron 35.0%
TGATCAGGAGTGAGTAATTA+TGG - chr6_3:89486898-89486917 Msa0963930:intron 35.0%
TTGAAGTTGGATAATTCTGC+TGG - chr6_3:89487006-89487025 Msa0963930:intron 35.0%
TGGATAATTCTGCTGGAAAA+AGG - chr6_3:89487013-89487032 Msa0963930:intron 35.0%
!! TGTTTGGAGAAAAGTGCTTA+TGG - chr6_3:89487190-89487209 Msa0963930:intron 35.0%
AGTACATCATGAGCTGAATT+GGG - chr6_3:89487213-89487232 Msa0963930:intron 35.0%
GAACTTATATAACGATGACG+AGG + chr6_3:89487478-89487497 None:intergenic 35.0%
!! TCATGGATTGGATTTGGATT+CGG - chr6_3:89487496-89487515 Msa0963930:intron 35.0%
ATTGGATTTGGATTCGGATT+TGG - chr6_3:89487502-89487521 Msa0963930:intron 35.0%
!!! ACGCGATTTGGATTTTGATT+TGG - chr6_3:89487676-89487695 Msa0963930:CDS 35.0%
! GTGGATTTTCATGCGTATAA+AGG - chr6_3:89488491-89488510 Msa0963930:five_prime_UTR 35.0%
AAATGAAAGAGTAAGCCACA+AGG + chr6_3:89488643-89488662 None:intergenic 35.0%
CGTGAGATAGAGAGAAAGAA+AGG + chr6_3:89486247-89486266 None:intergenic 40.0%
GCATGCTTATGTTGCTGAAA+TGG - chr6_3:89486477-89486496 Msa0963930:intron 40.0%
! GACTTTAGGGACTGTATTTG+AGG - chr6_3:89486514-89486533 Msa0963930:intron 40.0%
TAGGGACTGTATTTGAGGAA+AGG - chr6_3:89486519-89486538 Msa0963930:intron 40.0%
AAGGAGGTAGAACTCAAGTT+AGG - chr6_3:89486538-89486557 Msa0963930:intron 40.0%
AGGAGGTAGAACTCAAGTTA+GGG - chr6_3:89486539-89486558 Msa0963930:intron 40.0%
! AGTTAGGGTTTTGCACAACT+TGG - chr6_3:89486554-89486573 Msa0963930:intron 40.0%
CTTGGAATTGAAGATGTAGG+AGG - chr6_3:89486572-89486591 Msa0963930:intron 40.0%
! GAAATTGTTGCTGTGCTGTT+TGG - chr6_3:89486774-89486793 Msa0963930:intron 40.0%
CTAGAAGCCATAAGCTGTTT+GGG - chr6_3:89486806-89486825 Msa0963930:intron 40.0%
TAGAAGCCATAAGCTGTTTG+GGG - chr6_3:89486807-89486826 Msa0963930:intron 40.0%
!! TTCGGACAGGTTCTGTTTTT+GGG - chr6_3:89486857-89486876 Msa0963930:intron 40.0%
CTCCTGATCATCACAGTAAA+AGG + chr6_3:89486888-89486907 None:intergenic 40.0%
TTCTGCTGGAAAAAGGCTTA+CGG - chr6_3:89487020-89487039 Msa0963930:intron 40.0%
GAGTACATCATGAGCTGAAT+TGG - chr6_3:89487212-89487231 Msa0963930:intron 40.0%
ACTCGGAAAAATGCCAAGAT+TGG - chr6_3:89487254-89487273 Msa0963930:intron 40.0%
TGATACTTTGCCTGCTACTA+CGG - chr6_3:89487282-89487301 Msa0963930:intron 40.0%
AAGCAGTTGGAGAGAAAATG+TGG - chr6_3:89487305-89487324 Msa0963930:intron 40.0%
GGTAGAATTACCCCATTAGA+TGG - chr6_3:89487874-89487893 Msa0963930:five_prime_UTR 40.0%
GAAAAGAGCTATCCGTTAGA+TGG - chr6_3:89487996-89488015 Msa0963930:five_prime_UTR 40.0%
GAAAAGAGCTATCCGTTAGA+TGG - chr6_3:89487996-89488015 Msa0963930:five_prime_UTR 40.0%
AACTAGAAGGGAAACACACA+TGG + chr6_3:89488593-89488612 None:intergenic 40.0%
ATGTGTGTTTCCCTTCTAGT+TGG - chr6_3:89488592-89488611 Msa0963930:five_prime_UTR 40.0%
! AAGTTCATCTGTGTCACTCT+TGG - chr6_3:89488717-89488736 Msa0963930:five_prime_UTR 40.0%
! GGAAAGCTTTGGCAAGGATT+TGG + chr6_3:89486306-89486325 None:intergenic 45.0%
CCAAGAGGAAGGAGTGAATT+CGG + chr6_3:89486414-89486433 None:intergenic 45.0%
GGACTGTATTTGAGGAAAGG+AGG - chr6_3:89486522-89486541 Msa0963930:intron 45.0%
GCTAGAAGCCATAAGCTGTT+TGG - chr6_3:89486805-89486824 Msa0963930:intron 45.0%
ATCTTGCCCCAAACAGCTTA+TGG + chr6_3:89486816-89486835 None:intergenic 45.0%
! GTTCGGACAGGTTCTGTTTT+TGG - chr6_3:89486856-89486875 Msa0963930:intron 45.0%
! TCGGAAAGTGCGTTTGAAGT+TGG - chr6_3:89486993-89487012 Msa0963930:intron 45.0%
TCTGCTGGAAAAAGGCTTAC+GGG - chr6_3:89487021-89487040 Msa0963930:intron 45.0%
GGTTATAATGACGACGACCT+AGG - chr6_3:89487827-89487846 Msa0963930:CDS 45.0%
AATCACTACTAGGTGCACCT+AGG + chr6_3:89487847-89487866 None:intergenic 45.0%
TAATGGTGGCCTTTCCATAG+GGG + chr6_3:89487905-89487924 None:intergenic 45.0%
CTAATGGTGGCCTTTCCATA+GGG + chr6_3:89487906-89487925 None:intergenic 45.0%
TAATGGTGGCCTATCCATAG+GGG + chr6_3:89488027-89488046 None:intergenic 45.0%
CTAATGGTGGCCTATCCATA+GGG + chr6_3:89488028-89488047 None:intergenic 45.0%
TAATGGTGGCCTTTCCATAG+GGG + chr6_3:89487905-89487924 None:intergenic 45.0%
CTAATGGTGGCCTTTCCATA+GGG + chr6_3:89487906-89487925 None:intergenic 45.0%
! TGTGTTTCCCTTCTAGTTGG+TGG - chr6_3:89488595-89488614 Msa0963930:five_prime_UTR 45.0%
ACAACCACCACCAACTAGAA+GGG + chr6_3:89488605-89488624 None:intergenic 45.0%
AACAACCACCACCAACTAGA+AGG + chr6_3:89488606-89488625 None:intergenic 45.0%
TGTCTCTCACTAAGTCCTTG+TGG - chr6_3:89488625-89488644 Msa0963930:five_prime_UTR 45.0%
! TTCATCTGTGTCACTCTTGG+TGG - chr6_3:89488720-89488739 Msa0963930:five_prime_UTR 45.0%
!! ATAATAATAATTAAAATAAG+CGG + chr6_3:89487368-89487387 None:intergenic 5.0%
AGAGAGATTGGACAAGGGAG+GGG + chr6_3:89486274-89486293 None:intergenic 50.0%
GAGAGAGATTGGACAAGGGA+GGG + chr6_3:89486275-89486294 None:intergenic 50.0%
TGAGAGAGATTGGACAAGGG+AGG + chr6_3:89486276-89486295 None:intergenic 50.0%
TTTCCCTTCCTCTTCCTTGC+TGG - chr6_3:89486388-89486407 Msa0963930:intron 50.0%
CCGAATTCACTCCTTCCTCT+TGG - chr6_3:89486411-89486430 Msa0963930:intron 50.0%
!! TGTTGCTGTGCTGTTTGGGA+TGG - chr6_3:89486779-89486798 Msa0963930:intron 50.0%
CCTAATGGTGGCCTTTCCAT+AGG + chr6_3:89487907-89487926 None:intergenic 50.0%
CCTATGGAAAGGCCACCATT+AGG - chr6_3:89487904-89487923 Msa0963930:five_prime_UTR 50.0%
ATGGAAAGGCCACCATTAGG+AGG - chr6_3:89487907-89487926 Msa0963930:five_prime_UTR 50.0%
TGATAGCCCTCTCCTCCTAA+TGG + chr6_3:89487922-89487941 None:intergenic 50.0%
AGGAGAGGGCTATCAATGAG+AGG - chr6_3:89487927-89487946 Msa0963930:five_prime_UTR 50.0%
CCTAATGGTGGCCTATCCAT+AGG + chr6_3:89488029-89488048 None:intergenic 50.0%
CCTATGGATAGGCCACCATT+AGG - chr6_3:89488026-89488045 Msa0963930:five_prime_UTR 50.0%
ATGGATAGGCCACCATTAGG+AGG - chr6_3:89488029-89488048 Msa0963930:five_prime_UTR 50.0%
TGATAGCCCTCTCCTCCTAA+TGG + chr6_3:89487922-89487941 None:intergenic 50.0%
CCTAATGGTGGCCTTTCCAT+AGG + chr6_3:89487907-89487926 None:intergenic 50.0%
CCTATGGAAAGGCCACCATT+AGG - chr6_3:89487904-89487923 Msa0963930:five_prime_UTR 50.0%
ATGGAAAGGCCACCATTAGG+AGG - chr6_3:89487907-89487926 Msa0963930:five_prime_UTR 50.0%
!! GTTTCCCTTCTAGTTGGTGG+TGG - chr6_3:89488598-89488617 Msa0963930:five_prime_UTR 50.0%
GAATTCGGCCAGCAAGGAAG+AGG + chr6_3:89486399-89486418 None:intergenic 55.0%
AGGAGTGAATTCGGCCAGCA+AGG + chr6_3:89486405-89486424 None:intergenic 55.0%
CTCGAGCAGCGTGGATAACT+CGG - chr6_3:89487237-89487256 Msa0963930:intron 55.0%
CCAACTGCTTCCGTAGTAGC+AGG + chr6_3:89487295-89487314 None:intergenic 55.0%
CCTGCTACTACGGAAGCAGT+TGG - chr6_3:89487292-89487311 Msa0963930:intron 55.0%
TAGGGGCGGCTCCATCTAAT+GGG + chr6_3:89487888-89487907 None:intergenic 55.0%
ATAGGGGCGGCTCCATCTAA+TGG + chr6_3:89487889-89487908 None:intergenic 55.0%
ATTAGATGGAGCCGCCCCTA+TGG - chr6_3:89487888-89487907 Msa0963930:five_prime_UTR 55.0%
AAGGCCACCATTAGGAGGAG+AGG - chr6_3:89487912-89487931 Msa0963930:five_prime_UTR 55.0%
AGGCCACCATTAGGAGGAGA+GGG - chr6_3:89487913-89487932 Msa0963930:five_prime_UTR 55.0%
TAGCCCTCTCCTCCTAATGG+TGG + chr6_3:89487919-89487938 None:intergenic 55.0%
ATAGGGGCGGCTCCATCTAA+CGG + chr6_3:89487889-89487908 None:intergenic 55.0%
TAGGCCACCATTAGGAGGAG+AGG - chr6_3:89488034-89488053 Msa0963930:five_prime_UTR 55.0%
AGGCCACCATTAGGAGGAGA+GGG - chr6_3:89487913-89487932 Msa0963930:five_prime_UTR 55.0%
TAGCCCTCTCCTCCTAATGG+TGG + chr6_3:89487919-89487938 None:intergenic 55.0%
GGAGAGGGCTATCAACGAGA+TGG - chr6_3:89488050-89488069 Msa0963930:five_prime_UTR 55.0%
ATAGGGGCGGCTCCATCTAA+CGG + chr6_3:89487889-89487908 None:intergenic 55.0%
AAGGCCACCATTAGGAGGAG+AGG - chr6_3:89487912-89487931 Msa0963930:five_prime_UTR 55.0%
AGGCCACCATTAGGAGGAGA+GGG - chr6_3:89487913-89487932 Msa0963930:five_prime_UTR 55.0%
TGAGAGCCCTCTCCTCCTAA+TGG + chr6_3:89488205-89488224 None:intergenic 55.0%
CGGCCAGCAAGGAAGAGGAA+GGG + chr6_3:89486394-89486413 None:intergenic 60.0%
TCGGCCAGCAAGGAAGAGGA+AGG + chr6_3:89486395-89486414 None:intergenic 60.0%
GAATTGGGACTCGAGCAGCG+TGG - chr6_3:89487228-89487247 Msa0963930:intron 60.0%
AGGGGCGGCTCCATCTAATG+GGG + chr6_3:89487887-89487906 None:intergenic 60.0%
ATGGAGCCGCCCCTATGGAA+AGG - chr6_3:89487893-89487912 Msa0963930:five_prime_UTR 60.0%
TGGTGGCCTTTCCATAGGGG+CGG + chr6_3:89487902-89487921 None:intergenic 60.0%
GTTAGATGGAGCCGCCCCTA+TGG - chr6_3:89488010-89488029 Msa0963930:five_prime_UTR 60.0%
ATGGAGCCGCCCCTATGGAT+AGG - chr6_3:89488015-89488034 Msa0963930:five_prime_UTR 60.0%
TGGTGGCCTATCCATAGGGG+CGG + chr6_3:89488024-89488043 None:intergenic 60.0%
GTTAGATGGAGCCGCCCCTA+TGG - chr6_3:89488010-89488029 Msa0963930:five_prime_UTR 60.0%
ATGGAGCCGCCCCTATGGAA+AGG - chr6_3:89487893-89487912 Msa0963930:five_prime_UTR 60.0%
TGGTGGCCTTTCCATAGGGG+CGG + chr6_3:89487902-89487921 None:intergenic 60.0%
GAGCCCTCTCCTCCTAATGG+TGG + chr6_3:89488202-89488221 None:intergenic 60.0%
GGAGAGGGCTCTCAACGAGA+TGG - chr6_3:89488211-89488230 Msa0963930:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 89486221 89488745 89486221 ID=Msa0963930;Name=Msa0963930
chr6_3 mRNA 89486221 89488745 89486221 ID=Msa0963930-mRNA-1;Parent=Msa0963930;Name=Msa0963930-mRNA-1;_AED=0.00;_eAED=0.00;_QI=897|1|0.5|1|1|1|2|0|97
chr6_3 exon 89486221 89486288 89486221 ID=Msa0963930-mRNA-1:exon:16172;Parent=Msa0963930-mRNA-1
chr6_3 exon 89487623 89488745 89487623 ID=Msa0963930-mRNA-1:exon:16171;Parent=Msa0963930-mRNA-1
chr6_3 five_prime_UTR 89487849 89488745 89487849 ID=Msa0963930-mRNA-1:five_prime_utr;Parent=Msa0963930-mRNA-1
chr6_3 CDS 89487623 89487848 89487623 ID=Msa0963930-mRNA-1:cds;Parent=Msa0963930-mRNA-1
chr6_3 CDS 89486221 89486288 89486221 ID=Msa0963930-mRNA-1:cds;Parent=Msa0963930-mRNA-1
Gene Sequence

>Msa0963930

ATGATTATAAGAGTTTTGATCGAAGTTTTGGATGAATTCGGAACACGCAGAAGCCATAAGTTACAGTTAAGCTGTTTGGAGAAAAGTGCTTATGGAGTACATCATGAGCTGAATTGGGACTCGAGCAGCGTGGATAACTCGGAAAAATGCCAAGATTGGATAGTTGATACTTTGCCTGCTACTACGGAAGCAGTTGGAGAGAAAATGTGGTTGATTCGACATAGCTATTTTCAAGCAGCTAAGTATAAAGATGATAGTAGACTTGAAGTTCATCTGTGTCACTCTTGGTGGTAG
Protein sequence

>Msa0963930

MIIRVLIEVLDEFGTRRSHKLQLSCLEKSAYGVHHELNWDSSSVDNSEKCQDWIVDTLPATTEAVGEKMWLIRHSYFQAAKYKDDSRLEVHLCHSWW*