Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0967660 | XP_013461431.1 | 93.671 | 79 | 4 | 1 | 1 | 78 | 1 | 79 | 1.02e-45 | 150 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0069260 | Msa0967660 | 0.804932 | 1.703558e-49 | -8.615850e-47 |
| Msa0069310 | Msa0967660 | 0.808423 | 3.119465e-50 | -8.615850e-47 |
| Msa0721150 | Msa0967660 | 0.806326 | 8.685350e-50 | -8.615850e-47 |
| Msa0842240 | Msa0967660 | 0.802154 | 6.419720e-49 | -8.615850e-47 |
| Msa0376460 | Msa0967660 | 0.998257 | 4.522303e-260 | -8.615850e-47 |
| Msa0425110 | Msa0967660 | 0.901143 | 3.607490e-78 | -8.615850e-47 |
| Msa0471730 | Msa0967660 | 0.999093 | 7.628212e-290 | -8.615850e-47 |
| Msa0516500 | Msa0967660 | 0.980295 | 6.869365e-150 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0967660 | MtrunA17_Chr3g0127111 | 93.671 | 79 | 4 | 1 | 1 | 78 | 1 | 79 | 9.36e-50 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGGTTTCTTTGAATGCTT+TGG | 0.177524 | 6_3:-97739676 | None:intergenic |
| TTCTTTGAATGCTTTGGGTT+GGG | 0.313836 | 6_3:-97739670 | None:intergenic |
| ATTTGTTTACCTGAAGATAA+AGG | 0.340472 | 6_3:-97739116 | None:intergenic |
| CAGGTTTCTTTGAATGCTTT+GGG | 0.352628 | 6_3:-97739675 | None:intergenic |
| AGGTATTAATAAATAAAGTA+TGG | 0.361284 | 6_3:+97739904 | Msa0967660:three_prime_UTR |
| AAACCAAGAGGATGGCTAAA+AGG | 0.364847 | 6_3:-97739082 | None:intergenic |
| TGATTAGTAATTAGTTGTTA+AGG | 0.392811 | 6_3:-97739715 | None:intergenic |
| TTTCTTTGAATGCTTTGGGT+TGG | 0.396872 | 6_3:-97739671 | None:intergenic |
| TTGTAAAGAAAGTTATTAGT+AGG | 0.421064 | 6_3:-97739251 | None:intergenic |
| TACTAATAACTTTCTTTACA+AGG | 0.464774 | 6_3:+97739253 | Msa0967660:CDS |
| TGAGTGTATTGTAACTTATG+AGG | 0.474983 | 6_3:+97739884 | Msa0967660:three_prime_UTR |
| GGTGTTTAACGACCCTTGCC+AGG | 0.478184 | 6_3:-97739694 | None:intergenic |
| TTCATTGCATATACTTGCCA+TGG | 0.509554 | 6_3:+97739040 | Msa0967660:exon |
| ATGAATATAAATGACTTGGC+AGG | 0.510347 | 6_3:-97738975 | None:intergenic |
| CCAAAGCATTCAAAGAAACC+TGG | 0.549609 | 6_3:+97739676 | Msa0967660:CDS |
| AATCGAGCTAGTTTCTTCAC+CGG | 0.549992 | 6_3:+97739851 | Msa0967660:three_prime_UTR |
| TAAAATGAATATAAATGACT+TGG | 0.575545 | 6_3:-97738979 | None:intergenic |
| ATGAATGAAAACCAAGAGGA+TGG | 0.580545 | 6_3:-97739090 | None:intergenic |
| ATACACTCAAGTAGTGGCTC+CGG | 0.581091 | 6_3:-97739870 | None:intergenic |
| GTGTTGGATTATGATGAAGC+AGG | 0.583814 | 6_3:+97739646 | Msa0967660:CDS |
| GCATTCAAAGAAACCTGGCA+AGG | 0.596187 | 6_3:+97739681 | Msa0967660:CDS |
| CATTCAAAGAAACCTGGCAA+GGG | 0.630511 | 6_3:+97739682 | Msa0967660:CDS |
| GTTACAATACACTCAAGTAG+TGG | 0.639021 | 6_3:-97739876 | None:intergenic |
| GAAAATGAATGAAAACCAAG+AGG | 0.671422 | 6_3:-97739094 | None:intergenic |
| ATGGTGTAAAGATGAACTCG+AGG | 0.686471 | 6_3:+97739602 | Msa0967660:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTCATATAGTTTTAAA+TGG | + | chr6_3:97739288-97739307 | Msa0967660:intron | 10.0% |
| !!! | TTATTTGTAAACATTATTTT+GGG | - | chr6_3:97739463-97739482 | None:intergenic | 10.0% |
| !!! | TTTATTTGTAAACATTATTT+TGG | - | chr6_3:97739464-97739483 | None:intergenic | 10.0% |
| !!! | GATTACAAATTTTTTTTTAT+AGG | - | chr6_3:97739498-97739517 | None:intergenic | 10.0% |
| !!! | TTTTTTCATATAGTTTTAAA+TGG | + | chr6_3:97739288-97739307 | Msa0967660:intron | 10.0% |
| !!! | TTATTTGTAAACATTATTTT+GGG | - | chr6_3:97739463-97739482 | None:intergenic | 10.0% |
| !!! | TTTATTTGTAAACATTATTT+TGG | - | chr6_3:97739464-97739483 | None:intergenic | 10.0% |
| !!! | GATTACAAATTTTTTTTTAT+AGG | - | chr6_3:97739498-97739517 | None:intergenic | 10.0% |
| !! | TAAAATGAATATAAATGACT+TGG | - | chr6_3:97738982-97739001 | None:intergenic | 15.0% |
| !!! | ATTTATTTTTTTGCTCTTTT+AGG | + | chr6_3:97739197-97739216 | Msa0967660:intron | 15.0% |
| !! | AGGTATTAATAAATAAAGTA+TGG | + | chr6_3:97739904-97739923 | Msa0967660:three_prime_UTR | 15.0% |
| !! | TAAAATGAATATAAATGACT+TGG | - | chr6_3:97738982-97739001 | None:intergenic | 15.0% |
| !!! | ATTTATTTTTTTGCTCTTTT+AGG | + | chr6_3:97739197-97739216 | Msa0967660:intron | 15.0% |
| !! | AGGTATTAATAAATAAAGTA+TGG | + | chr6_3:97739904-97739923 | Msa0967660:three_prime_UTR | 15.0% |
| !! | AAATTCTTACTTGATATAAG+TGG | + | chr6_3:97739135-97739154 | Msa0967660:intron | 20.0% |
| !! | TTGTAAAGAAAGTTATTAGT+AGG | - | chr6_3:97739254-97739273 | None:intergenic | 20.0% |
| !! | TACTAATAACTTTCTTTACA+AGG | + | chr6_3:97739253-97739272 | Msa0967660:CDS | 20.0% |
| !! | CTATATGAAAAAAGATAGTA+AGG | - | chr6_3:97739281-97739300 | None:intergenic | 20.0% |
| !! | AAAATTAAGATGAATACAGA+TGG | + | chr6_3:97739372-97739391 | Msa0967660:intron | 20.0% |
| !!! | ACATTATTTTGGGATTATAT+GGG | - | chr6_3:97739453-97739472 | None:intergenic | 20.0% |
| !!! | AACATTATTTTGGGATTATA+TGG | - | chr6_3:97739454-97739473 | None:intergenic | 20.0% |
| !!! | TTTTTAACTAACAAGTACTT+TGG | + | chr6_3:97739566-97739585 | Msa0967660:intron | 20.0% |
| !! | TGATTAGTAATTAGTTGTTA+AGG | - | chr6_3:97739718-97739737 | None:intergenic | 20.0% |
| !! | AAATTCTTACTTGATATAAG+TGG | + | chr6_3:97739135-97739154 | Msa0967660:intron | 20.0% |
| !! | TTGTAAAGAAAGTTATTAGT+AGG | - | chr6_3:97739254-97739273 | None:intergenic | 20.0% |
| !! | TACTAATAACTTTCTTTACA+AGG | + | chr6_3:97739253-97739272 | Msa0967660:CDS | 20.0% |
| !! | CTATATGAAAAAAGATAGTA+AGG | - | chr6_3:97739281-97739300 | None:intergenic | 20.0% |
| !! | AAAATTAAGATGAATACAGA+TGG | + | chr6_3:97739372-97739391 | Msa0967660:intron | 20.0% |
| !!! | ACATTATTTTGGGATTATAT+GGG | - | chr6_3:97739453-97739472 | None:intergenic | 20.0% |
| !!! | AACATTATTTTGGGATTATA+TGG | - | chr6_3:97739454-97739473 | None:intergenic | 20.0% |
| !!! | TTTTTAACTAACAAGTACTT+TGG | + | chr6_3:97739566-97739585 | Msa0967660:intron | 20.0% |
| !! | TGATTAGTAATTAGTTGTTA+AGG | - | chr6_3:97739718-97739737 | None:intergenic | 20.0% |
| !! | ATTCATTTTCCTTTATCTTC+AGG | + | chr6_3:97739107-97739126 | Msa0967660:CDS | 25.0% |
| ! | ATTTGTTTACCTGAAGATAA+AGG | - | chr6_3:97739119-97739138 | None:intergenic | 25.0% |
| !!! | TTTTTTTGCTCTTTTAGGAT+TGG | + | chr6_3:97739202-97739221 | Msa0967660:intron | 25.0% |
| !! | TATGTTCATCTTAAGTATTG+TGG | - | chr6_3:97739340-97739359 | None:intergenic | 25.0% |
| !! | ACATGAACAAAACATTTTGA+GGG | - | chr6_3:97739799-97739818 | None:intergenic | 25.0% |
| !! | TACATGAACAAAACATTTTG+AGG | - | chr6_3:97739800-97739819 | None:intergenic | 25.0% |
| !!! | AAATGTTTTGTTCATGTAAG+AGG | + | chr6_3:97739802-97739821 | Msa0967660:three_prime_UTR | 25.0% |
| !! | ATTCATTTTCCTTTATCTTC+AGG | + | chr6_3:97739107-97739126 | Msa0967660:CDS | 25.0% |
| ! | ATTTGTTTACCTGAAGATAA+AGG | - | chr6_3:97739119-97739138 | None:intergenic | 25.0% |
| !!! | TTTTTTTGCTCTTTTAGGAT+TGG | + | chr6_3:97739202-97739221 | Msa0967660:intron | 25.0% |
| !! | TATGTTCATCTTAAGTATTG+TGG | - | chr6_3:97739340-97739359 | None:intergenic | 25.0% |
| !! | ACATGAACAAAACATTTTGA+GGG | - | chr6_3:97739799-97739818 | None:intergenic | 25.0% |
| !! | TACATGAACAAAACATTTTG+AGG | - | chr6_3:97739800-97739819 | None:intergenic | 25.0% |
| !!! | AAATGTTTTGTTCATGTAAG+AGG | + | chr6_3:97739802-97739821 | Msa0967660:three_prime_UTR | 25.0% |
| ! | ATGAATATAAATGACTTGGC+AGG | - | chr6_3:97738978-97738997 | None:intergenic | 30.0% |
| !!! | TTTTAGCTTCTCATAAAGAG+AGG | + | chr6_3:97738997-97739016 | Msa0967660:five_prime_UTR | 30.0% |
| GAAAATGAATGAAAACCAAG+AGG | - | chr6_3:97739097-97739116 | None:intergenic | 30.0% | |
| ! | AGTACTTTGGTATTGAATGA+AGG | + | chr6_3:97739579-97739598 | Msa0967660:intron | 30.0% |
| ! | CATGAACAAAACATTTTGAG+GGG | - | chr6_3:97739798-97739817 | None:intergenic | 30.0% |
| TGAGTGTATTGTAACTTATG+AGG | + | chr6_3:97739884-97739903 | Msa0967660:three_prime_UTR | 30.0% | |
| ! | ATGAATATAAATGACTTGGC+AGG | - | chr6_3:97738978-97738997 | None:intergenic | 30.0% |
| !!! | TTTTAGCTTCTCATAAAGAG+AGG | + | chr6_3:97738997-97739016 | Msa0967660:five_prime_UTR | 30.0% |
| GAAAATGAATGAAAACCAAG+AGG | - | chr6_3:97739097-97739116 | None:intergenic | 30.0% | |
| ! | AGTACTTTGGTATTGAATGA+AGG | + | chr6_3:97739579-97739598 | Msa0967660:intron | 30.0% |
| ! | CATGAACAAAACATTTTGAG+GGG | - | chr6_3:97739798-97739817 | None:intergenic | 30.0% |
| TGAGTGTATTGTAACTTATG+AGG | + | chr6_3:97739884-97739903 | Msa0967660:three_prime_UTR | 30.0% | |
| TTCATTGCATATACTTGCCA+TGG | + | chr6_3:97739040-97739059 | Msa0967660:exon | 35.0% | |
| !!! | AGTTTTGACTTTGTGTTCCA+TGG | - | chr6_3:97739060-97739079 | None:intergenic | 35.0% |
| ATGAATGAAAACCAAGAGGA+TGG | - | chr6_3:97739093-97739112 | None:intergenic | 35.0% | |
| GGCAAATGCTAATATTTGCT+TGG | + | chr6_3:97739415-97739434 | Msa0967660:intron | 35.0% | |
| ! | CTTTGGTATTGAATGAAGGA+TGG | + | chr6_3:97739583-97739602 | Msa0967660:intron | 35.0% |
| ! | ATTGTCTCATGCTTTTGTGT+TGG | + | chr6_3:97739630-97739649 | Msa0967660:CDS | 35.0% |
| ! | TTCTTTGAATGCTTTGGGTT+GGG | - | chr6_3:97739673-97739692 | None:intergenic | 35.0% |
| ! | TTTCTTTGAATGCTTTGGGT+TGG | - | chr6_3:97739674-97739693 | None:intergenic | 35.0% |
| CAGGTTTCTTTGAATGCTTT+GGG | - | chr6_3:97739678-97739697 | None:intergenic | 35.0% | |
| GTTACAATACACTCAAGTAG+TGG | - | chr6_3:97739879-97739898 | None:intergenic | 35.0% | |
| TTCATTGCATATACTTGCCA+TGG | + | chr6_3:97739040-97739059 | Msa0967660:exon | 35.0% | |
| !!! | AGTTTTGACTTTGTGTTCCA+TGG | - | chr6_3:97739060-97739079 | None:intergenic | 35.0% |
| ATGAATGAAAACCAAGAGGA+TGG | - | chr6_3:97739093-97739112 | None:intergenic | 35.0% | |
| GGCAAATGCTAATATTTGCT+TGG | + | chr6_3:97739415-97739434 | Msa0967660:intron | 35.0% | |
| ! | CTTTGGTATTGAATGAAGGA+TGG | + | chr6_3:97739583-97739602 | Msa0967660:intron | 35.0% |
| ! | ATTGTCTCATGCTTTTGTGT+TGG | + | chr6_3:97739630-97739649 | Msa0967660:CDS | 35.0% |
| ! | TTCTTTGAATGCTTTGGGTT+GGG | - | chr6_3:97739673-97739692 | None:intergenic | 35.0% |
| ! | TTTCTTTGAATGCTTTGGGT+TGG | - | chr6_3:97739674-97739693 | None:intergenic | 35.0% |
| CAGGTTTCTTTGAATGCTTT+GGG | - | chr6_3:97739678-97739697 | None:intergenic | 35.0% | |
| GTTACAATACACTCAAGTAG+TGG | - | chr6_3:97739879-97739898 | None:intergenic | 35.0% | |
| ! | TTTCCTTTTAGCCATCCTCT+TGG | + | chr6_3:97739079-97739098 | Msa0967660:CDS | 40.0% |
| AAACCAAGAGGATGGCTAAA+AGG | - | chr6_3:97739085-97739104 | None:intergenic | 40.0% | |
| GAGCTACTACATTCACATTG+AGG | + | chr6_3:97739394-97739413 | Msa0967660:intron | 40.0% | |
| ATGGTGTAAAGATGAACTCG+AGG | + | chr6_3:97739602-97739621 | Msa0967660:CDS | 40.0% | |
| GTGTTGGATTATGATGAAGC+AGG | + | chr6_3:97739646-97739665 | Msa0967660:CDS | 40.0% | |
| CCAGGTTTCTTTGAATGCTT+TGG | - | chr6_3:97739679-97739698 | None:intergenic | 40.0% | |
| ! | CCAAAGCATTCAAAGAAACC+TGG | + | chr6_3:97739676-97739695 | Msa0967660:CDS | 40.0% |
| CATTCAAAGAAACCTGGCAA+GGG | + | chr6_3:97739682-97739701 | Msa0967660:CDS | 40.0% | |
| ! | AATCGAGCTAGTTTCTTCAC+CGG | + | chr6_3:97739851-97739870 | Msa0967660:three_prime_UTR | 40.0% |
| ! | TTTCCTTTTAGCCATCCTCT+TGG | + | chr6_3:97739079-97739098 | Msa0967660:CDS | 40.0% |
| AAACCAAGAGGATGGCTAAA+AGG | - | chr6_3:97739085-97739104 | None:intergenic | 40.0% | |
| GAGCTACTACATTCACATTG+AGG | + | chr6_3:97739394-97739413 | Msa0967660:intron | 40.0% | |
| ATGGTGTAAAGATGAACTCG+AGG | + | chr6_3:97739602-97739621 | Msa0967660:CDS | 40.0% | |
| GTGTTGGATTATGATGAAGC+AGG | + | chr6_3:97739646-97739665 | Msa0967660:CDS | 40.0% | |
| CCAGGTTTCTTTGAATGCTT+TGG | - | chr6_3:97739679-97739698 | None:intergenic | 40.0% | |
| ! | CCAAAGCATTCAAAGAAACC+TGG | + | chr6_3:97739676-97739695 | Msa0967660:CDS | 40.0% |
| CATTCAAAGAAACCTGGCAA+GGG | + | chr6_3:97739682-97739701 | Msa0967660:CDS | 40.0% | |
| ! | AATCGAGCTAGTTTCTTCAC+CGG | + | chr6_3:97739851-97739870 | Msa0967660:three_prime_UTR | 40.0% |
| !!! | GCTCTTTTAGGATTGGCTGA+AGG | + | chr6_3:97739209-97739228 | Msa0967660:intron | 45.0% |
| GCATTCAAAGAAACCTGGCA+AGG | + | chr6_3:97739681-97739700 | Msa0967660:CDS | 45.0% | |
| ATACACTCAAGTAGTGGCTC+CGG | - | chr6_3:97739873-97739892 | None:intergenic | 45.0% | |
| !!! | GCTCTTTTAGGATTGGCTGA+AGG | + | chr6_3:97739209-97739228 | Msa0967660:intron | 45.0% |
| GCATTCAAAGAAACCTGGCA+AGG | + | chr6_3:97739681-97739700 | Msa0967660:CDS | 45.0% | |
| ATACACTCAAGTAGTGGCTC+CGG | - | chr6_3:97739873-97739892 | None:intergenic | 45.0% | |
| GGTGTTTAACGACCCTTGCC+AGG | - | chr6_3:97739697-97739716 | None:intergenic | 55.0% | |
| GGTGTTTAACGACCCTTGCC+AGG | - | chr6_3:97739697-97739716 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 97738970 | 97739939 | 97738970 | ID=Msa0967660;Name=Msa0967660 |
| chr6_3 | mRNA | 97738970 | 97739939 | 97738970 | ID=Msa0967660-mRNA-1;Parent=Msa0967660;Name=Msa0967660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=89|1|1|1|1|1|3|228|78 |
| chr6_3 | exon | 97738970 | 97739128 | 97738970 | ID=Msa0967660-mRNA-1:exon:18173;Parent=Msa0967660-mRNA-1 |
| chr6_3 | exon | 97739219 | 97739274 | 97739219 | ID=Msa0967660-mRNA-1:exon:18174;Parent=Msa0967660-mRNA-1 |
| chr6_3 | exon | 97739601 | 97739939 | 97739601 | ID=Msa0967660-mRNA-1:exon:18175;Parent=Msa0967660-mRNA-1 |
| chr6_3 | five_prime_UTR | 97738970 | 97739058 | 97738970 | ID=Msa0967660-mRNA-1:five_prime_utr;Parent=Msa0967660-mRNA-1 |
| chr6_3 | CDS | 97739059 | 97739128 | 97739059 | ID=Msa0967660-mRNA-1:cds;Parent=Msa0967660-mRNA-1 |
| chr6_3 | CDS | 97739219 | 97739274 | 97739219 | ID=Msa0967660-mRNA-1:cds;Parent=Msa0967660-mRNA-1 |
| chr6_3 | CDS | 97739601 | 97739711 | 97739601 | ID=Msa0967660-mRNA-1:cds;Parent=Msa0967660-mRNA-1 |
| chr6_3 | three_prime_UTR | 97739712 | 97739939 | 97739712 | ID=Msa0967660-mRNA-1:three_prime_utr;Parent=Msa0967660-mRNA-1 |
| Gene Sequence |
| Protein sequence |
