AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0976260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0976260 MtrunA17_Chr6g0455291 90.258 503 40 1 1 503 1 494 0.0 875
Msa0976260 MtrunA17_Chr2g0296981 69.034 507 139 4 5 503 1 497 0.0 665
Msa0976260 MtrunA17_Chr4g0052711 47.523 444 207 6 77 499 42 480 2.06e-134 397
Msa0976260 MtrunA17_Chr2g0297001 53.590 390 158 7 101 471 78 463 8.23e-132 391
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0976260 AT4G20050.5 61.111 450 172 3 54 501 58 506 0.0 550
Msa0976260 AT4G20050.7 60.976 451 173 3 53 501 32 481 0.0 549
Msa0976260 AT4G20050.6 60.976 451 173 3 53 501 32 481 0.0 549
Msa0976260 AT4G20050.2 60.976 451 173 3 53 501 32 481 0.0 549
Msa0976260 AT4G20050.1 60.976 451 173 3 53 501 32 481 0.0 549
Msa0976260 AT4G20050.4 65.365 384 130 3 120 501 1 383 1.95e-179 509
Msa0976260 AT4G20050.3 65.365 384 130 3 120 501 1 383 1.95e-179 509
Msa0976260 AT4G20040.1 49.512 410 183 4 102 493 74 477 2.66e-131 390

Find 85 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAATTGATTTGTCAAATTT+TGG 0.107009 6_4:-10155095 Msa0976260:CDS
ACTTGTTCAATTTGTTTAAA+AGG 0.165481 6_4:+10153695 None:intergenic
AATATTGAACATTTCTAATA+AGG 0.212684 6_4:+10153552 None:intergenic
TTACCTTTAAGCACTATTTC+TGG 0.223377 6_4:-10156548 Msa0976260:CDS
CCACCAGAAATAGTGCTTAA+AGG 0.277659 6_4:+10156545 None:intergenic
GGTCAAGCTAACATAATTTC+TGG 0.300088 6_4:-10154744 Msa0976260:CDS
TGTGAATGGTATGAATTTAA+AGG 0.300917 6_4:-10153654 Msa0976260:CDS
TATAATAAAGCTAGTGGATT+TGG 0.307725 6_4:-10154711 Msa0976260:CDS
TATATTAGAAACACTTTCAT+TGG 0.350508 6_4:-10154897 Msa0976260:CDS
TCAATCAAAGGCATAGTTAA+TGG 0.354427 6_4:-10153794 Msa0976260:CDS
ATAACACATTTCACTACAAA+TGG 0.355300 6_4:-10154948 Msa0976260:CDS
ATGATATCTATGCATGGAAA+TGG 0.386160 6_4:-10153620 Msa0976260:CDS
TGAACATTTCTAATAAGGTT+AGG 0.388575 6_4:+10153557 None:intergenic
ATTGTTGACAACATGTTCTC+TGG 0.409396 6_4:-10153764 Msa0976260:CDS
GGAATCATGTTTGGTTAAAG+AGG 0.421087 6_4:+10156481 None:intergenic
CTTGAGATGTGAAGTTGAAC+AGG 0.422590 6_4:+10153854 None:intergenic
GGAGAGGGTGAGAGTGAAGA+TGG 0.434607 6_4:+10156446 None:intergenic
ACATTTCTAAGAGCATGATT+AGG 0.437297 6_4:+10153503 None:intergenic
GTGCTATCTGAATTTCCTGT+TGG 0.443841 6_4:+10156208 None:intergenic
TAATTGCATTATCATTCCCT+TGG 0.444714 6_4:+10154811 None:intergenic
ATCTCAAGTAGTTTCTTCCT+TGG 0.448224 6_4:-10153839 Msa0976260:CDS
CTGCAAAAGGTCCAAGTAAA+GGG 0.456444 6_4:-10156156 Msa0976260:CDS
GGTGCTCAAATTAATCTTCA+AGG 0.476483 6_4:-10156103 Msa0976260:CDS
TATATGTTTCATGACCACTT+TGG 0.482051 6_4:+10154916 None:intergenic
TGAAGCAATGGAATCATGTT+TGG 0.485230 6_4:+10156472 None:intergenic
CTCATAATTGTAGGAGGGTG+AGG 0.486040 6_4:+10155067 None:intergenic
TGCATGGAAATGGAACTTCA+TGG 0.488929 6_4:-10153610 Msa0976260:CDS
GTTGCAAGAAATGTTGTGAA+TGG 0.497121 6_4:-10153668 Msa0976260:CDS
GCTGCAAAAGGTCCAAGTAA+AGG 0.500398 6_4:-10156157 Msa0976260:CDS
TCATATGGTGCTGATCCAAC+AGG 0.501553 6_4:-10156223 Msa0976260:CDS
AAGGATTGTAAATTCTTACA+TGG 0.506307 6_4:-10153903 Msa0976260:CDS
GTTTGATGGAAAATCGTTTG+AGG 0.510842 6_4:+10155124 None:intergenic
AATAAAGCTAGTGGATTTGG+TGG 0.511486 6_4:-10154708 Msa0976260:CDS
GCCATAGCTGATGCTGCAAA+AGG 0.511601 6_4:-10156169 Msa0976260:CDS
TGGACTTATTCTTGCCATTG+TGG 0.512010 6_4:+10156631 None:intergenic
TGTTATGTACTCATAATTGT+AGG 0.514253 6_4:+10155058 None:intergenic
ATGAATGGCATAATTGACCT+TGG 0.516824 6_4:-10156127 Msa0976260:CDS
GCTAGTGGATTTGGTGGCAC+AGG 0.519295 6_4:-10154702 Msa0976260:CDS
ATGTTCTCTGGATCAAATCA+TGG 0.519341 6_4:-10153752 Msa0976260:CDS
GACTCAAACTTCAAAGGTGG+AGG 0.519564 6_4:-10155017 Msa0976260:CDS
TTCTCCTATGTCATCATCAC+AGG 0.523692 6_4:-10156393 Msa0976260:intron
GATATTGGAATTATGATTAG+TGG 0.527008 6_4:-10154765 Msa0976260:CDS
GGCATTGGAATTAGTCTCCA+AGG 0.529041 6_4:-10154828 Msa0976260:CDS
GAGTACCTTGTGACATCATA+TGG 0.530416 6_4:-10156238 Msa0976260:CDS
TTTCTAGACTCAAACTTCAA+AGG 0.534336 6_4:-10155023 Msa0976260:CDS
CAATATTCTTTAAGTGCTAG+TGG 0.539169 6_4:-10153536 Msa0976260:CDS
CCTTTAAGCACTATTTCTGG+TGG 0.542078 6_4:-10156545 Msa0976260:CDS
TGAAGATGGTGGTGAAGCAA+TGG 0.546527 6_4:+10156460 None:intergenic
CTAGGAGATGACATAGGAGA+AGG 0.548634 6_4:+10156419 None:intergenic
CAACAGGTTTAACACAAACA+AGG 0.552410 6_4:-10153922 Msa0976260:intron
ATTTGATGTTAACAGATACA+TGG 0.552529 6_4:-10155161 Msa0976260:intron
ATTGGTCAACACATAACTGC+AGG 0.563405 6_4:-10154879 Msa0976260:CDS
AGATTTCCAAAACCAGCCAC+TGG 0.570556 6_4:+10156046 None:intergenic
TATGTACTCATAATTGTAGG+AGG 0.570702 6_4:+10155061 None:intergenic
CATTGCTATAATAAAGCTAG+TGG 0.574442 6_4:-10154717 Msa0976260:CDS
ATAGGAGAAGGGCTAGGAGA+GGG 0.577444 6_4:+10156431 None:intergenic
TGTGATGATGACATAGGAGA+AGG 0.592994 6_4:+10156395 None:intergenic
GATTAATTTGAGCACCTCCA+AGG 0.593703 6_4:+10156110 None:intergenic
GGAATTTACTTGAAACTACC+AGG 0.595914 6_4:-10154681 Msa0976260:intron
ATTCTTCTAAAATCAATCAA+AGG 0.599646 6_4:-10153806 Msa0976260:CDS
GATGACATAGGAGAAGGGCT+AGG 0.601891 6_4:+10156425 None:intergenic
CTAGACTCAAACTTCAAAGG+TGG 0.602601 6_4:-10155020 Msa0976260:CDS
GATGACATAGGAGAAGGGCT+AGG 0.609601 6_4:+10156401 None:intergenic
CCAGCCACTGGAAAATGCAA+AGG 0.626588 6_4:+10156058 None:intergenic
GAGGGTGAGAGTGAAGATGG+TGG 0.629564 6_4:+10156449 None:intergenic
TAGGAGATGACATAGGAGAA+GGG 0.631480 6_4:+10156420 None:intergenic
ATTACCTGTGATGATGACAT+AGG 0.631918 6_4:+10156389 None:intergenic
GTGATGATGACATAGGAGAA+GGG 0.635434 6_4:+10156396 None:intergenic
ATCAGAAGCATGAACCACAA+TGG 0.637219 6_4:+10156611 None:intergenic
TCAGCAATGATATCTATGCA+TGG 0.639349 6_4:-10153626 Msa0976260:CDS
GCATTGGAATTAGTCTCCAA+GGG 0.641926 6_4:-10154827 Msa0976260:CDS
CATAGGAGAAGGGCTAGGAG+AGG 0.643169 6_4:+10156430 None:intergenic
CAGCACCATATGATGTCACA+AGG 0.645343 6_4:+10156233 None:intergenic
GAAGAATCTTTGCATCACCA+AGG 0.647203 6_4:+10153822 None:intergenic
GAAGGGCTAGGAGATGACAT+AGG 0.651600 6_4:+10156413 None:intergenic
ATGTACTCATAATTGTAGGA+GGG 0.651633 6_4:+10155062 None:intergenic
GCTCAAATTAATCTTCAAGG+TGG 0.652201 6_4:-10156100 Msa0976260:CDS
TGGCAAGAATAAGTCCATTG+TGG 0.661515 6_4:-10156625 Msa0976260:CDS
GCATGAGTTACAAAGAAACA+AGG 0.666734 6_4:+10156584 None:intergenic
GGTCAACACATAACTGCAGG+AGG 0.681897 6_4:-10154876 Msa0976260:CDS
AATGGCATAATTGACCTTGG+AGG 0.687495 6_4:-10156124 Msa0976260:CDS
GATGTCACAAGGTACTCATG+TGG 0.688833 6_4:+10156244 None:intergenic
TGTTGTGATTGAAACAAGTG+TGG 0.691769 6_4:-10153468 Msa0976260:CDS
TCACCAAATGAAAACCACAA+TGG 0.717638 6_4:-10156645 None:intergenic
TGATGTTAACAGATACATGG+AGG 0.722635 6_4:-10155158 Msa0976260:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAATTTTGTTAAAATTTTA+AGG - chr6_4:10154133-10154152 Msa0976260:intron 10.0%
!! GATTATTTATATTAAAGTTA+AGG - chr6_4:10155420-10155439 Msa0976260:intron 10.0%
!! TTGAATGTTTAAAAAAAAAT+TGG + chr6_4:10155633-10155652 None:intergenic 10.0%
!!! AAAAATGAAAGAAAATTTTC+AGG - chr6_4:10155207-10155226 Msa0976260:intron 15.0%
!!! TTATAATGTCAAACTTTTAT+CGG + chr6_4:10155536-10155555 None:intergenic 15.0%
!!! GTTTGACATTATAATTTTAA+AGG - chr6_4:10155542-10155561 Msa0976260:intron 15.0%
!! AGAAATTATCATTAATTAAG+TGG + chr6_4:10155606-10155625 None:intergenic 15.0%
!! ATGTTTAAAAAAAAATTGGA+AGG + chr6_4:10155629-10155648 None:intergenic 15.0%
!! ATAGTGATTAAAATAAAACA+AGG + chr6_4:10155671-10155690 None:intergenic 15.0%
!!! ACTTTTTTTAAGTAGTTTAA+TGG + chr6_4:10156013-10156032 None:intergenic 15.0%
!! AATATTGAACATTTCTAATA+AGG + chr6_4:10156507-10156526 None:intergenic 15.0%
!! TTAGAAAAATATGGATGATA+CGG - chr6_4:10153719-10153738 Msa0976260:CDS 20.0%
!!! TAGAATTTTAATGTTAATGC+AGG - chr6_4:10153778-10153797 Msa0976260:CDS 20.0%
!!! AATTTTTGTAAAAACCACTA+TGG - chr6_4:10154275-10154294 Msa0976260:intron 20.0%
!!! AAAGTCTTTTGTCTATAAAA+AGG + chr6_4:10154358-10154377 None:intergenic 20.0%
!! ATTTCAAACATATATCTGTT+TGG + chr6_4:10154578-10154597 None:intergenic 20.0%
!!! TCAATTAGATTTTTGTTTGA+TGG + chr6_4:10154949-10154968 None:intergenic 20.0%
!!! CTAATTGATTTGTCAAATTT+TGG - chr6_4:10154961-10154980 Msa0976260:CDS 20.0%
!! TATATTAGAAACACTTTCAT+TGG - chr6_4:10155159-10155178 Msa0976260:intron 20.0%
!! TATAATCAAAAACAATTACC+TGG + chr6_4:10155396-10155415 None:intergenic 20.0%
!! AAAACATGATATATTTGCAA+TGG + chr6_4:10155462-10155481 None:intergenic 20.0%
!!! CATTATAATTTTAAAGGATG+AGG - chr6_4:10155548-10155567 Msa0976260:intron 20.0%
!!! ATTCTTCTAAAATCAATCAA+AGG - chr6_4:10156250-10156269 Msa0976260:CDS 20.0%
!!! ACTTGTTCAATTTGTTTAAA+AGG + chr6_4:10156364-10156383 None:intergenic 20.0%
!!! TATTTTTCTAAGGATGAGTT+AGG + chr6_4:10153710-10153729 None:intergenic 25.0%
! AACTCATCCTTAGAAAAATA+TGG - chr6_4:10153710-10153729 Msa0976260:CDS 25.0%
!! GTATCATCCATATTTTTCTA+AGG + chr6_4:10153720-10153739 None:intergenic 25.0%
! ACCATTCTTGGTAAATTATT+AGG - chr6_4:10154051-10154070 Msa0976260:intron 25.0%
! ACCTAATAATTTACCAAGAA+TGG + chr6_4:10154055-10154074 None:intergenic 25.0%
! CGATTTCAAACATATTTGTT+TGG + chr6_4:10154201-10154220 None:intergenic 25.0%
! AAAATTATGGTATACCATAG+TGG + chr6_4:10154292-10154311 None:intergenic 25.0%
! CGTTTCGATTGAAAAAATTA+TGG + chr6_4:10154305-10154324 None:intergenic 25.0%
!! TTTGAAGTTAATATGAGTCA+AGG - chr6_4:10154380-10154399 Msa0976260:intron 25.0%
!!! TATTGACTCAAGAAAAGAAA+GGG + chr6_4:10154424-10154443 None:intergenic 25.0%
!!! ATATTGACTCAAGAAAAGAA+AGG + chr6_4:10154425-10154444 None:intergenic 25.0%
!! ATGTCAAATTTGATGTCTTT+TGG + chr6_4:10154460-10154479 None:intergenic 25.0%
!! CAAGGTAAAGTACATTATTT+TGG - chr6_4:10154799-10154818 Msa0976260:CDS 25.0%
!! ATGTCAAATTTGATGTCTTT+TGG + chr6_4:10154460-10154479 None:intergenic 25.0%
! ATTTGATGTTAACAGATACA+TGG - chr6_4:10154895-10154914 Msa0976260:CDS 25.0%
! TGTTATGTACTCATAATTGT+AGG + chr6_4:10155001-10155020 None:intergenic 25.0%
! ATAACACATTTCACTACAAA+TGG - chr6_4:10155108-10155127 Msa0976260:CDS 25.0%
! GATATTGGAATTATGATTAG+TGG - chr6_4:10155291-10155310 Msa0976260:intron 25.0%
!!! TATAATAAAGCTAGTGGATT+TGG - chr6_4:10155345-10155364 Msa0976260:intron 25.0%
! TTATCGGTAAAAAAAAACAG+TGG + chr6_4:10155520-10155539 None:intergenic 25.0%
! GACTAAAATGGATATTAAGA+GGG - chr6_4:10155754-10155773 Msa0976260:intron 25.0%
!! CAACTTTGTCAAATTTTACA+AGG - chr6_4:10155784-10155803 Msa0976260:intron 25.0%
! AAATTTGCACATCTTAAATG+TGG + chr6_4:10155988-10156007 None:intergenic 25.0%
! GACATGTAATAAATTCATGT+AGG + chr6_4:10156106-10156125 None:intergenic 25.0%
! AAGGATTGTAAATTCTTACA+TGG - chr6_4:10156153-10156172 Msa0976260:CDS 25.0%
! TGTGAATGGTATGAATTTAA+AGG - chr6_4:10156402-10156421 Msa0976260:CDS 25.0%
! TGAACATTTCTAATAAGGTT+AGG + chr6_4:10156502-10156521 None:intergenic 25.0%
TTACCTTTAAGCACTATTTC+TGG - chr6_4:10153508-10153527 Msa0976260:CDS 30.0%
TTCATCAAAAACCCTTTACT+TGG + chr6_4:10153914-10153933 None:intergenic 30.0%
! AGTAAAGGGTTTTTGATGAA+TGG - chr6_4:10153914-10153933 Msa0976260:CDS 30.0%
AAGTTTGAAGAAACCATTCT+TGG - chr6_4:10154039-10154058 Msa0976260:intron 30.0%
TCGCTGTAAAAACTTTATGA+AGG + chr6_4:10154109-10154128 None:intergenic 30.0%
AAGTGAGTTTGACAAAATCA+TGG + chr6_4:10154688-10154707 None:intergenic 30.0%
CTTCTTAGTCTAAGAAACTT+TGG + chr6_4:10154768-10154787 None:intergenic 30.0%
! TCAAATTTGATGTCTTTTGG+AGG + chr6_4:10154457-10154476 None:intergenic 30.0%
ATGTACTCATAATTGTAGGA+GGG + chr6_4:10154997-10155016 None:intergenic 30.0%
TATGTACTCATAATTGTAGG+AGG + chr6_4:10154998-10155017 None:intergenic 30.0%
TTTCTAGACTCAAACTTCAA+AGG - chr6_4:10155033-10155052 Msa0976260:CDS 30.0%
!! AATGGAATTTTAGTCCAAAG+TGG - chr6_4:10155126-10155145 Msa0976260:CDS 30.0%
TATATGTTTCATGACCACTT+TGG + chr6_4:10155143-10155162 None:intergenic 30.0%
!! GAAAGAAAATTTTCAGGCAT+TGG - chr6_4:10155213-10155232 Msa0976260:intron 30.0%
TAATTGCATTATCATTCCCT+TGG + chr6_4:10155248-10155267 None:intergenic 30.0%
!!! GTGATTTTTTCTGCTGATAT+TGG - chr6_4:10155276-10155295 Msa0976260:intron 30.0%
CATTGCTATAATAAAGCTAG+TGG - chr6_4:10155339-10155358 Msa0976260:intron 30.0%
GGTTCAAATCTCATTTAAGA+CGG - chr6_4:10155569-10155588 Msa0976260:intron 30.0%
GGACTAAAATGGATATTAAG+AGG - chr6_4:10155753-10155772 Msa0976260:intron 30.0%
TATCAACTAAGCTACACTTA+CGG + chr6_4:10155906-10155925 None:intergenic 30.0%
! AAAATTTGGGGTTCAAATCT+CGG + chr6_4:10155957-10155976 None:intergenic 30.0%
TGTGGAGAAGTGAAAAATTT+GGG + chr6_4:10155970-10155989 None:intergenic 30.0%
ATGTGGAGAAGTGAAAAATT+TGG + chr6_4:10155971-10155990 None:intergenic 30.0%
! CTTAAAAAAAGTGCGAAACA+TGG - chr6_4:10156021-10156040 Msa0976260:intron 30.0%
! TACATGTCTAACTTTTCAAC+AGG - chr6_4:10156118-10156137 Msa0976260:CDS 30.0%
! TCAATCAAAGGCATAGTTAA+TGG - chr6_4:10156262-10156281 Msa0976260:intron 30.0%
ATGATATCTATGCATGGAAA+TGG - chr6_4:10156436-10156455 Msa0976260:CDS 30.0%
CAATATTCTTTAAGTGCTAG+TGG - chr6_4:10156520-10156539 Msa0976260:CDS 30.0%
! ACATTTCTAAGAGCATGATT+AGG + chr6_4:10156556-10156575 None:intergenic 30.0%
!! TGCAAATGTTTTTGTCAAAG+TGG - chr6_4:10156618-10156637 Msa0976260:CDS 30.0%
GCATGAGTTACAAAGAAACA+AGG + chr6_4:10153475-10153494 None:intergenic 35.0%
GGAATCATGTTTGGTTAAAG+AGG + chr6_4:10153578-10153597 None:intergenic 35.0%
TGAAGCAATGGAATCATGTT+TGG + chr6_4:10153587-10153606 None:intergenic 35.0%
! ATTACCTGTGATGATGACAT+AGG + chr6_4:10153670-10153689 None:intergenic 35.0%
ATGAATGGCATAATTGACCT+TGG - chr6_4:10153929-10153948 Msa0976260:intron 35.0%
GGTGCTCAAATTAATCTTCA+AGG - chr6_4:10153953-10153972 Msa0976260:intron 35.0%
GCTCAAATTAATCTTCAAGG+TGG - chr6_4:10153956-10153975 Msa0976260:intron 35.0%
TAAGAAGTTGCAGCATATCA+AGG - chr6_4:10154781-10154800 Msa0976260:CDS 35.0%
TGATGTTAACAGATACATGG+AGG - chr6_4:10154898-10154917 Msa0976260:CDS 35.0%
GTTTGATGGAAAATCGTTTG+AGG + chr6_4:10154935-10154954 None:intergenic 35.0%
GGTCAAGCTAACATAATTTC+TGG - chr6_4:10155312-10155331 Msa0976260:intron 35.0%
!! AATAAAGCTAGTGGATTTGG+TGG - chr6_4:10155348-10155367 Msa0976260:intron 35.0%
GGAATTTACTTGAAACTACC+AGG - chr6_4:10155375-10155394 Msa0976260:intron 35.0%
AGAATCAAGTGCATGTTACA+AGG + chr6_4:10155719-10155738 None:intergenic 35.0%
TGATTCTAGTCCTCAAAATG+AGG - chr6_4:10155732-10155751 Msa0976260:intron 35.0%
!! CCATTTTAGTCCTCATTTTG+AGG + chr6_4:10155745-10155764 None:intergenic 35.0%
CCTCAAAATGAGGACTAAAA+TGG - chr6_4:10155742-10155761 Msa0976260:intron 35.0%
ATCAACTAAGCTACACTTAC+GGG + chr6_4:10155905-10155924 None:intergenic 35.0%
GTGGAGAAGTGAAAAATTTG+GGG + chr6_4:10155969-10155988 None:intergenic 35.0%
CAACAGGTTTAACACAAACA+AGG - chr6_4:10156134-10156153 Msa0976260:CDS 35.0%
ATCTCAAGTAGTTTCTTCCT+TGG - chr6_4:10156217-10156236 Msa0976260:CDS 35.0%
ATTGTTGACAACATGTTCTC+TGG - chr6_4:10156292-10156311 Msa0976260:intron 35.0%
ATGTTCTCTGGATCAAATCA+TGG - chr6_4:10156304-10156323 Msa0976260:intron 35.0%
GTTGCAAGAAATGTTGTGAA+TGG - chr6_4:10156388-10156407 Msa0976260:intron 35.0%
TCAGCAATGATATCTATGCA+TGG - chr6_4:10156430-10156449 Msa0976260:CDS 35.0%
TGTTGTGATTGAAACAAGTG+TGG - chr6_4:10156588-10156607 Msa0976260:CDS 35.0%
TGGACTTATTCTTGCCATTG+TGG + chr6_4:10153428-10153447 None:intergenic 40.0%
TGGCAAGAATAAGTCCATTG+TGG - chr6_4:10153431-10153450 Msa0976260:CDS 40.0%
! ATCAGAAGCATGAACCACAA+TGG + chr6_4:10153448-10153467 None:intergenic 40.0%
CCACCAGAAATAGTGCTTAA+AGG + chr6_4:10153514-10153533 None:intergenic 40.0%
!! CCTTTAAGCACTATTTCTGG+TGG - chr6_4:10153511-10153530 Msa0976260:CDS 40.0%
TAGGAGATGACATAGGAGAA+GGG + chr6_4:10153639-10153658 None:intergenic 40.0%
! GTGATGATGACATAGGAGAA+GGG + chr6_4:10153663-10153682 None:intergenic 40.0%
! TGTGATGATGACATAGGAGA+AGG + chr6_4:10153664-10153683 None:intergenic 40.0%
TTCTCCTATGTCATCATCAC+AGG - chr6_4:10153663-10153682 Msa0976260:CDS 40.0%
GAGTACCTTGTGACATCATA+TGG - chr6_4:10153818-10153837 Msa0976260:CDS 40.0%
GTGCTATCTGAATTTCCTGT+TGG + chr6_4:10153851-10153870 None:intergenic 40.0%
CTGCAAAAGGTCCAAGTAAA+GGG - chr6_4:10153900-10153919 Msa0976260:CDS 40.0%
AATGGCATAATTGACCTTGG+AGG - chr6_4:10153932-10153951 Msa0976260:intron 40.0%
! GATTAATTTGAGCACCTCCA+AGG + chr6_4:10153949-10153968 None:intergenic 40.0%
! CAAACCTTTGCATTTTCCAG+TGG - chr6_4:10153994-10154013 Msa0976260:intron 40.0%
!!! GGCTGGTTTTGGAAATCTTA+TGG - chr6_4:10154015-10154034 Msa0976260:intron 40.0%
CTAGACTCAAACTTCAAAGG+TGG - chr6_4:10155036-10155055 Msa0976260:CDS 40.0%
ATTGGTCAACACATAACTGC+AGG - chr6_4:10155177-10155196 Msa0976260:intron 40.0%
! GCATTGGAATTAGTCTCCAA+GGG - chr6_4:10155229-10155248 Msa0976260:intron 40.0%
! CTGAGGTATTATTTGCGCAA+GGG - chr6_4:10155931-10155950 Msa0976260:intron 40.0%
GCGAAACATGGTAAATTTGC+AGG - chr6_4:10156033-10156052 Msa0976260:intron 40.0%
CTTGAGATGTGAAGTTGAAC+AGG + chr6_4:10156205-10156224 None:intergenic 40.0%
GAAGAATCTTTGCATCACCA+AGG + chr6_4:10156237-10156256 None:intergenic 40.0%
TGCATGGAAATGGAACTTCA+TGG - chr6_4:10156446-10156465 Msa0976260:CDS 40.0%
! TGAAGATGGTGGTGAAGCAA+TGG + chr6_4:10153599-10153618 None:intergenic 45.0%
CTAGGAGATGACATAGGAGA+AGG + chr6_4:10153640-10153659 None:intergenic 45.0%
! GATGTCACAAGGTACTCATG+TGG + chr6_4:10153815-10153834 None:intergenic 45.0%
CAGCACCATATGATGTCACA+AGG + chr6_4:10153826-10153845 None:intergenic 45.0%
!! TCATATGGTGCTGATCCAAC+AGG - chr6_4:10153833-10153852 Msa0976260:CDS 45.0%
! ACCTTTTGCAGCATCAGCTA+TGG + chr6_4:10153891-10153910 None:intergenic 45.0%
GCTGCAAAAGGTCCAAGTAA+AGG - chr6_4:10153899-10153918 Msa0976260:CDS 45.0%
!!! CATTTTCCAGTGGCTGGTTT+TGG - chr6_4:10154004-10154023 Msa0976260:intron 45.0%
AGATTTCCAAAACCAGCCAC+TGG + chr6_4:10154013-10154032 None:intergenic 45.0%
CTCATAATTGTAGGAGGGTG+AGG + chr6_4:10154992-10155011 None:intergenic 45.0%
GACTCAAACTTCAAAGGTGG+AGG - chr6_4:10155039-10155058 Msa0976260:CDS 45.0%
! GGCATTGGAATTAGTCTCCA+AGG - chr6_4:10155228-10155247 Msa0976260:intron 45.0%
!! GCTTAGTTGATAGCTACCTG+AGG - chr6_4:10155914-10155933 Msa0976260:intron 45.0%
CCTTGCGCAAATAATACCTC+AGG + chr6_4:10155933-10155952 None:intergenic 45.0%
! CCTGAGGTATTATTTGCGCA+AGG - chr6_4:10155930-10155949 Msa0976260:intron 45.0%
!! AAATTTATTTATATTTGAAT+TGG - chr6_4:10155692-10155711 Msa0976260:intron 5.0%
ATAGGAGAAGGGCTAGGAGA+GGG + chr6_4:10153628-10153647 None:intergenic 50.0%
GATGACATAGGAGAAGGGCT+AGG + chr6_4:10153634-10153653 None:intergenic 50.0%
GAAGGGCTAGGAGATGACAT+AGG + chr6_4:10153646-10153665 None:intergenic 50.0%
GATGACATAGGAGAAGGGCT+AGG + chr6_4:10153634-10153653 None:intergenic 50.0%
GCCATAGCTGATGCTGCAAA+AGG - chr6_4:10153887-10153906 Msa0976260:CDS 50.0%
CCAGCCACTGGAAAATGCAA+AGG + chr6_4:10154001-10154020 None:intergenic 50.0%
! CCTTTGCATTTTCCAGTGGC+TGG - chr6_4:10153998-10154017 Msa0976260:intron 50.0%
GGTCAACACATAACTGCAGG+AGG - chr6_4:10155180-10155199 Msa0976260:intron 50.0%
GAGGGTGAGAGTGAAGATGG+TGG + chr6_4:10153610-10153629 None:intergenic 55.0%
GGAGAGGGTGAGAGTGAAGA+TGG + chr6_4:10153613-10153632 None:intergenic 55.0%
CATAGGAGAAGGGCTAGGAG+AGG + chr6_4:10153629-10153648 None:intergenic 55.0%
!! GCTAGTGGATTTGGTGGCAC+AGG - chr6_4:10155354-10155373 Msa0976260:intron 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 10153418 10156660 10153418 ID=Msa0976260;Name=Msa0976260
chr6_4 mRNA 10153418 10156660 10153418 ID=Msa0976260-mRNA-1;Parent=Msa0976260;Name=Msa0976260-mRNA-1;_AED=0.04;_eAED=0.05;_QI=0|0.66|0.25|1|1|1|4|0|503
chr6_4 exon 10156394 10156660 10156394 ID=Msa0976260-mRNA-1:exon:3731;Parent=Msa0976260-mRNA-1
chr6_4 exon 10156042 10156278 10156042 ID=Msa0976260-mRNA-1:exon:3730;Parent=Msa0976260-mRNA-1
chr6_4 exon 10154682 10155168 10154682 ID=Msa0976260-mRNA-1:exon:3729;Parent=Msa0976260-mRNA-1
chr6_4 exon 10153418 10153938 10153418 ID=Msa0976260-mRNA-1:exon:3728;Parent=Msa0976260-mRNA-1
chr6_4 CDS 10156394 10156660 10156394 ID=Msa0976260-mRNA-1:cds;Parent=Msa0976260-mRNA-1
chr6_4 CDS 10156042 10156278 10156042 ID=Msa0976260-mRNA-1:cds;Parent=Msa0976260-mRNA-1
chr6_4 CDS 10154682 10155168 10154682 ID=Msa0976260-mRNA-1:cds;Parent=Msa0976260-mRNA-1
chr6_4 CDS 10153418 10153938 10153418 ID=Msa0976260-mRNA-1:cds;Parent=Msa0976260-mRNA-1
Gene Sequence

>Msa0976260

ATGAAAACCACAATGGCAAGAATAAGTCCATTGTGGTTCATGCTTCTGATTATGCCTTGTTTCTTTGTAACTCATGCTTATAAAGTTAAGTTACCTTTAAGCACTATTTCTGGTGGAACTCATCATGATGCAATGCTTAAATTTAATGCATTCAAAGCCTCTTTAACCAAACATGATTCCATTGCTTCACCACCATCTTCACTCTCACCCTCTCCTAGCCCTTCTCCTATGTCATCTCCTAGCCCTTCTCCTATGTCATCATCACAGGATCTGAAGAAACCACATGAGTACCTTGTGACATCATATGGTGCTGATCCAACAGGAAATTCAGATAGCACTGAAGCACTTCTTGCAGCCATAGCTGATGCTGCAAAAGGTCCAAGTAAAGGGTTTTTGATGAATGGCATAATTGACCTTGGAGGTGCTCAAATTAATCTTCAAGGTGGAAGTTACTTGATTAGCAAACCTTTGCATTTTCCAGTGGCTGGTTTTGGAAATCTTATGATACATGGAGGAACAATAAAAGCCTCAAACGATTTTCCATCAAACAAAAATCTAATTGATTTGTCAAATTTTGGTTCAACCTCACCCTCCTACAATTATGAGTACATAACACTCAAAGATCTCTTTCTAGACTCAAACTTCAAAGGTGGAGGAATTTCAATCACAAATTCACTAAGAATCAACATAGACAATTGTTACATAACACATTTCACTACAAATGGAATTTTAGTCCAAAGTGGTCATGAAACATATATTAGAAACACTTTCATTGGTCAACACATAACTGCAGGAGGTGACAAAAATGAAAGAAAATTTTCAGGCATTGGAATTAGTCTCCAAGGGAATGATAATGCAATTACAGATGTTGTGATTTTTTCTGCTGATATTGGAATTATGATTAGTGGTCAAGCTAACATAATTTCTGGTGTACATTGCTATAATAAAGCTAGTGGATTTGGTGGCACAGGAATTTACTTGAAACTACCAGGTTTAACACAAACAAGGATTGTAAATTCTTACATGGATTACACAAACATTGTTGCTGAAGATCCTGTTCAACTTCACATCTCAAGTAGTTTCTTCCTTGGTGATGCAAAGATTCTTCTAAAATCAATCAAAGGCATAGTTAATGGTCTCAACATTGTTGACAACATGTTCTCTGGATCAAATCATGGTGATGAAATTGTGAAACTAGACACATCAAATTGTCCTTTTAAACAAATTGAACAAGTTTTTGTTGCAAGAAATGTTGTGAATGGTATGAATTTAAAGGCTACTTCAGCAATGATATCTATGCATGGAAATGGAACTTCATGGATTGCTGATTTTAACAAAATTTTGATTTTTCCTAACCTTATTAGAAATGTTCAATATTCTTTAAGTGCTAGTGGTAATACATTTCCTAATCATGCTCTTAGAAATGTGTCTCATAATATTGTTGTGATTGAAACAAGTGTGGCTGTGACTGCAAATGTTTTTGTCAAAGTGGATCAAAGTATTACAAGTTGA
Protein sequence

>Msa0976260

MKTTMARISPLWFMLLIMPCFFVTHAYKVKLPLSTISGGTHHDAMLKFNAFKASLTKHDSIASPPSSLSPSPSPSPMSSPSPSPMSSSQDLKKPHEYLVTSYGADPTGNSDSTEALLAAIADAAKGPSKGFLMNGIIDLGGAQINLQGGSYLISKPLHFPVAGFGNLMIHGGTIKASNDFPSNKNLIDLSNFGSTSPSYNYEYITLKDLFLDSNFKGGGISITNSLRINIDNCYITHFTTNGILVQSGHETYIRNTFIGQHITAGGDKNERKFSGIGISLQGNDNAITDVVIFSADIGIMISGQANIISGVHCYNKASGFGGTGIYLKLPGLTQTRIVNSYMDYTNIVAEDPVQLHISSSFFLGDAKILLKSIKGIVNGLNIVDNMFSGSNHGDEIVKLDTSNCPFKQIEQVFVARNVVNGMNLKATSAMISMHGNGTSWIADFNKILIFPNLIRNVQYSLSASGNTFPNHALRNVSHNIVVIETSVAVTANVFVKVDQSITS*