Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0979300 | AFK37645.1 | 97.600 | 125 | 3 | 0 | 1 | 125 | 1 | 125 | 9.00e-83 | 249 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0979300 | sp|Q0WPX7|BAG2_ARATH | 49.438 | 89 | 44 | 1 | 38 | 125 | 15 | 103 | 1.49e-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0979300 | I3SBK3 | 97.600 | 125 | 3 | 0 | 1 | 125 | 1 | 125 | 4.30e-83 | 249 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0876950 | Msa0979300 | 0.858406 | 8.421712e-63 | -8.615850e-47 |
| Msa0876960 | Msa0979300 | 0.947435 | 6.770456e-106 | -8.615850e-47 |
| Msa0979300 | Msa0979310 | 0.857224 | 1.887778e-62 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0979300 | MtrunA17_Chr6g0458271 | 97.600 | 125 | 3 | 0 | 1 | 125 | 1 | 125 | 8.37e-87 | 249 |
| Msa0979300 | MtrunA17_Chr6g0458281 | 88.889 | 126 | 9 | 3 | 1 | 125 | 1 | 122 | 4.93e-73 | 214 |
| Msa0979300 | MtrunA17_Chr6g0473621 | 45.217 | 115 | 55 | 4 | 19 | 125 | 11 | 125 | 6.73e-23 | 91.3 |
| Msa0979300 | MtrunA17_Chr7g0243051 | 43.299 | 97 | 52 | 1 | 32 | 125 | 13 | 109 | 1.23e-21 | 85.9 |
| Msa0979300 | MtrunA17_Chr2g0285591 | 43.617 | 94 | 52 | 1 | 33 | 125 | 25 | 118 | 6.31e-21 | 84.3 |
| Msa0979300 | MtrunA17_Chr1g0203771 | 43.434 | 99 | 52 | 2 | 31 | 125 | 15 | 113 | 9.01e-21 | 85.5 |
| Msa0979300 | MtrunA17_Chr4g0057261 | 45.556 | 90 | 47 | 1 | 38 | 125 | 32 | 121 | 2.06e-19 | 80.9 |
| Msa0979300 | MtrunA17_Chr4g0007601 | 50.000 | 68 | 34 | 0 | 58 | 125 | 26 | 93 | 3.20e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0979300 | AT5G14360.1 | 54.676 | 139 | 44 | 4 | 1 | 125 | 1 | 134 | 5.09e-46 | 146 |
| Msa0979300 | AT5G40630.1 | 45.517 | 145 | 58 | 4 | 1 | 125 | 1 | 144 | 2.43e-35 | 119 |
| Msa0979300 | AT5G62100.3 | 49.438 | 89 | 44 | 1 | 38 | 125 | 15 | 103 | 5.56e-21 | 82.8 |
| Msa0979300 | AT5G62100.6 | 49.438 | 89 | 44 | 1 | 38 | 125 | 15 | 103 | 5.56e-21 | 82.8 |
| Msa0979300 | AT5G62100.5 | 49.438 | 89 | 44 | 1 | 38 | 125 | 15 | 103 | 1.98e-20 | 82.8 |
| Msa0979300 | AT5G62100.4 | 49.438 | 89 | 44 | 1 | 38 | 125 | 15 | 103 | 2.57e-20 | 82.4 |
| Msa0979300 | AT5G62100.2 | 49.438 | 89 | 44 | 1 | 38 | 125 | 15 | 103 | 1.51e-19 | 81.6 |
| Msa0979300 | AT5G62100.1 | 49.438 | 89 | 44 | 1 | 38 | 125 | 15 | 103 | 2.44e-19 | 81.3 |
| Msa0979300 | AT5G07220.1 | 46.067 | 89 | 47 | 1 | 38 | 125 | 21 | 109 | 2.59e-19 | 81.3 |
| Msa0979300 | AT3G51780.1 | 40.952 | 105 | 47 | 1 | 36 | 125 | 8 | 112 | 8.40e-19 | 79.7 |
| Msa0979300 | AT5G52060.1 | 41.935 | 93 | 49 | 1 | 38 | 125 | 39 | 131 | 1.64e-17 | 77.0 |
Find 24 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGAAGCAACTTCAACTTT+TGG | 0.327143 | 6_4:+15958831 | Msa0979300:CDS |
| AGCATATCTAAACTTGGCTT+TGG | 0.330269 | 6_4:+15958639 | Msa0979300:CDS |
| TTCTTCACATGATTGGTGTT+AGG | 0.341495 | 6_4:+15959410 | Msa0979300:CDS |
| AATTTCAGGAGAATTGAAAA+TGG | 0.354607 | 6_4:+15959309 | Msa0979300:intron |
| TAGAGAAGCCTCTGCTCTCT+TGG | 0.368216 | 6_4:-15959358 | None:intergenic |
| TCACTACAATCCTTCTCAAT+TGG | 0.382631 | 6_4:-15958681 | None:intergenic |
| TCTTCACATGATTGGTGTTA+GGG | 0.396279 | 6_4:+15959411 | Msa0979300:CDS |
| AATGAATTTCTTCACATGAT+TGG | 0.414769 | 6_4:+15959403 | Msa0979300:CDS |
| AGAGAGAGTAACAAAAGTGT+TGG | 0.471291 | 6_4:+15958756 | Msa0979300:CDS |
| TGTAGAAGCATATCTAAACT+TGG | 0.485899 | 6_4:+15958633 | Msa0979300:CDS |
| TGAAAATAGTGAAATCGAAT+GGG | 0.493131 | 6_4:+15958701 | Msa0979300:CDS |
| GTGAAAATAGTGAAATCGAA+TGG | 0.497284 | 6_4:+15958700 | Msa0979300:CDS |
| ATCGAATGGGAACTTAGGCC+TGG | 0.546005 | 6_4:+15958714 | Msa0979300:CDS |
| AAAATAAGATGATGAAGTTG+AGG | 0.552877 | 6_4:+15958598 | None:intergenic |
| TGATGAAGTTGAGGTCAAAG+AGG | 0.559715 | 6_4:+15958607 | Msa0979300:CDS |
| GAGCAGAGGCTTCTCTACAA+AGG | 0.569914 | 6_4:+15959364 | Msa0979300:CDS |
| TTCTCTACAAAGGTAAAGAG+AGG | 0.582260 | 6_4:+15959374 | Msa0979300:CDS |
| GAGTTTCAACTATGTCAAAG+TGG | 0.599297 | 6_4:+15958796 | Msa0979300:CDS |
| AGTAGCATCTCCAATTGAGA+AGG | 0.608005 | 6_4:+15958671 | Msa0979300:CDS |
| GTGAAATCGAATGGGAACTT+AGG | 0.612081 | 6_4:+15958709 | Msa0979300:CDS |
| TGGTGTTAGGGACAAAGACA+AGG | 0.613407 | 6_4:+15959423 | Msa0979300:CDS |
| GTTTAGAGCCAAGAGAGCAG+AGG | 0.629869 | 6_4:+15959350 | Msa0979300:CDS |
| TCTCTACAAAGGTAAAGAGA+GGG | 0.650942 | 6_4:+15959375 | Msa0979300:CDS |
| GAATGGGAACTTAGGCCTGG+TGG | 0.713354 | 6_4:+15958717 | Msa0979300:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATTGAACAATTATATT+AGG | + | chr6_4:15958917-15958936 | Msa0979300:intron | 10.0% |
| !! | TTTAATTGAACAATTATATT+AGG | + | chr6_4:15958917-15958936 | Msa0979300:intron | 10.0% |
| !! | TTGTACATTAACATAAAATA+TGG | + | chr6_4:15958968-15958987 | Msa0979300:intron | 15.0% |
| !!! | AATTTTTATGAATGAATTTC+AGG | + | chr6_4:15959295-15959314 | Msa0979300:intron | 15.0% |
| !! | TTGTACATTAACATAAAATA+TGG | + | chr6_4:15958968-15958987 | Msa0979300:intron | 15.0% |
| !!! | AATTTTTATGAATGAATTTC+AGG | + | chr6_4:15959295-15959314 | Msa0979300:intron | 15.0% |
| !! | TAATTTAAACCCAAAACAAA+AGG | - | chr6_4:15958891-15958910 | None:intergenic | 20.0% |
| !!! | ATAATAATGCAAGACTTTTT+TGG | - | chr6_4:15959235-15959254 | None:intergenic | 20.0% |
| !! | CAACTATAAAAATTACTTTG+AGG | + | chr6_4:15959260-15959279 | Msa0979300:intron | 20.0% |
| !! | TAATTTAAACCCAAAACAAA+AGG | - | chr6_4:15958891-15958910 | None:intergenic | 20.0% |
| !!! | ATAATAATGCAAGACTTTTT+TGG | - | chr6_4:15959235-15959254 | None:intergenic | 20.0% |
| !! | CAACTATAAAAATTACTTTG+AGG | + | chr6_4:15959260-15959279 | Msa0979300:intron | 20.0% |
| ! | TGAAAATAGTGAAATCGAAT+GGG | + | chr6_4:15958701-15958720 | Msa0979300:CDS | 25.0% |
| ! | AATCAATACAAATGAAAGGT+TGG | - | chr6_4:15958867-15958886 | None:intergenic | 25.0% |
| !!! | TATTGATTGCCTTTTGTTTT+GGG | + | chr6_4:15958879-15958898 | Msa0979300:intron | 25.0% |
| ! | GAGTTATTACCAAAATTTGT+TGG | + | chr6_4:15958998-15959017 | Msa0979300:intron | 25.0% |
| ! | AATTTCAGGAGAATTGAAAA+TGG | + | chr6_4:15959309-15959328 | Msa0979300:intron | 25.0% |
| !!! | ATTGAAAATGGTTTTGTCAT+TGG | + | chr6_4:15959321-15959340 | Msa0979300:CDS | 25.0% |
| ! | AATGAATTTCTTCACATGAT+TGG | + | chr6_4:15959403-15959422 | Msa0979300:CDS | 25.0% |
| ! | TGAAAATAGTGAAATCGAAT+GGG | + | chr6_4:15958701-15958720 | Msa0979300:CDS | 25.0% |
| ! | AATCAATACAAATGAAAGGT+TGG | - | chr6_4:15958867-15958886 | None:intergenic | 25.0% |
| !!! | TATTGATTGCCTTTTGTTTT+GGG | + | chr6_4:15958879-15958898 | Msa0979300:intron | 25.0% |
| ! | GAGTTATTACCAAAATTTGT+TGG | + | chr6_4:15958998-15959017 | Msa0979300:intron | 25.0% |
| ! | AATTTCAGGAGAATTGAAAA+TGG | + | chr6_4:15959309-15959328 | Msa0979300:intron | 25.0% |
| !!! | ATTGAAAATGGTTTTGTCAT+TGG | + | chr6_4:15959321-15959340 | Msa0979300:CDS | 25.0% |
| ! | AATGAATTTCTTCACATGAT+TGG | + | chr6_4:15959403-15959422 | Msa0979300:CDS | 25.0% |
| ! | TGTAGAAGCATATCTAAACT+TGG | + | chr6_4:15958633-15958652 | Msa0979300:CDS | 30.0% |
| GTGAAAATAGTGAAATCGAA+TGG | + | chr6_4:15958700-15958719 | Msa0979300:CDS | 30.0% | |
| ! | ATTGAAGCAACTTCAACTTT+TGG | + | chr6_4:15958831-15958850 | Msa0979300:CDS | 30.0% |
| AGGCAATCAATACAAATGAA+AGG | - | chr6_4:15958871-15958890 | None:intergenic | 30.0% | |
| !!! | GTATTGATTGCCTTTTGTTT+TGG | + | chr6_4:15958878-15958897 | Msa0979300:intron | 30.0% |
| ! | AACTACATGCCAACAAATTT+TGG | - | chr6_4:15959010-15959029 | None:intergenic | 30.0% |
| !! | TCTCCATTTTTAAGAGTTAG+AGG | + | chr6_4:15959119-15959138 | Msa0979300:intron | 30.0% |
| !! | CTCCATTTTTAAGAGTTAGA+GGG | + | chr6_4:15959120-15959139 | Msa0979300:intron | 30.0% |
| AACCCTCTAACTCTTAAAAA+TGG | - | chr6_4:15959125-15959144 | None:intergenic | 30.0% | |
| ! | TGTAGAAGCATATCTAAACT+TGG | + | chr6_4:15958633-15958652 | Msa0979300:CDS | 30.0% |
| GTGAAAATAGTGAAATCGAA+TGG | + | chr6_4:15958700-15958719 | Msa0979300:CDS | 30.0% | |
| ! | ATTGAAGCAACTTCAACTTT+TGG | + | chr6_4:15958831-15958850 | Msa0979300:CDS | 30.0% |
| AGGCAATCAATACAAATGAA+AGG | - | chr6_4:15958871-15958890 | None:intergenic | 30.0% | |
| !!! | GTATTGATTGCCTTTTGTTT+TGG | + | chr6_4:15958878-15958897 | Msa0979300:intron | 30.0% |
| ! | AACTACATGCCAACAAATTT+TGG | - | chr6_4:15959010-15959029 | None:intergenic | 30.0% |
| !! | TCTCCATTTTTAAGAGTTAG+AGG | + | chr6_4:15959119-15959138 | Msa0979300:intron | 30.0% |
| !! | CTCCATTTTTAAGAGTTAGA+GGG | + | chr6_4:15959120-15959139 | Msa0979300:intron | 30.0% |
| AACCCTCTAACTCTTAAAAA+TGG | - | chr6_4:15959125-15959144 | None:intergenic | 30.0% | |
| AGCATATCTAAACTTGGCTT+TGG | + | chr6_4:15958639-15958658 | Msa0979300:CDS | 35.0% | |
| TCACTACAATCCTTCTCAAT+TGG | - | chr6_4:15958684-15958703 | None:intergenic | 35.0% | |
| ! | AGAGAGAGTAACAAAAGTGT+TGG | + | chr6_4:15958756-15958775 | Msa0979300:CDS | 35.0% |
| GAGTTTCAACTATGTCAAAG+TGG | + | chr6_4:15958796-15958815 | Msa0979300:CDS | 35.0% | |
| AGGGTTGAAAAAATTCTGCA+TGG | + | chr6_4:15959139-15959158 | Msa0979300:intron | 35.0% | |
| TTCTCTACAAAGGTAAAGAG+AGG | + | chr6_4:15959374-15959393 | Msa0979300:CDS | 35.0% | |
| TCTCTACAAAGGTAAAGAGA+GGG | + | chr6_4:15959375-15959394 | Msa0979300:CDS | 35.0% | |
| ! | TTCTTCACATGATTGGTGTT+AGG | + | chr6_4:15959410-15959429 | Msa0979300:CDS | 35.0% |
| !! | TCTTCACATGATTGGTGTTA+GGG | + | chr6_4:15959411-15959430 | Msa0979300:CDS | 35.0% |
| AGCATATCTAAACTTGGCTT+TGG | + | chr6_4:15958639-15958658 | Msa0979300:CDS | 35.0% | |
| TCACTACAATCCTTCTCAAT+TGG | - | chr6_4:15958684-15958703 | None:intergenic | 35.0% | |
| ! | AGAGAGAGTAACAAAAGTGT+TGG | + | chr6_4:15958756-15958775 | Msa0979300:CDS | 35.0% |
| GAGTTTCAACTATGTCAAAG+TGG | + | chr6_4:15958796-15958815 | Msa0979300:CDS | 35.0% | |
| AGGGTTGAAAAAATTCTGCA+TGG | + | chr6_4:15959139-15959158 | Msa0979300:intron | 35.0% | |
| TTCTCTACAAAGGTAAAGAG+AGG | + | chr6_4:15959374-15959393 | Msa0979300:CDS | 35.0% | |
| TCTCTACAAAGGTAAAGAGA+GGG | + | chr6_4:15959375-15959394 | Msa0979300:CDS | 35.0% | |
| ! | TTCTTCACATGATTGGTGTT+AGG | + | chr6_4:15959410-15959429 | Msa0979300:CDS | 35.0% |
| !! | TCTTCACATGATTGGTGTTA+GGG | + | chr6_4:15959411-15959430 | Msa0979300:CDS | 35.0% |
| TGATGAAGTTGAGGTCAAAG+AGG | + | chr6_4:15958607-15958626 | Msa0979300:CDS | 40.0% | |
| AGTAGCATCTCCAATTGAGA+AGG | + | chr6_4:15958671-15958690 | Msa0979300:CDS | 40.0% | |
| GTGAAATCGAATGGGAACTT+AGG | + | chr6_4:15958709-15958728 | Msa0979300:CDS | 40.0% | |
| TAATTCAAAGTTCCGCCGTA+CGG | - | chr6_4:15959094-15959113 | None:intergenic | 40.0% | |
| TGATGAAGTTGAGGTCAAAG+AGG | + | chr6_4:15958607-15958626 | Msa0979300:CDS | 40.0% | |
| AGTAGCATCTCCAATTGAGA+AGG | + | chr6_4:15958671-15958690 | Msa0979300:CDS | 40.0% | |
| GTGAAATCGAATGGGAACTT+AGG | + | chr6_4:15958709-15958728 | Msa0979300:CDS | 40.0% | |
| TAATTCAAAGTTCCGCCGTA+CGG | - | chr6_4:15959094-15959113 | None:intergenic | 40.0% | |
| ! | TTTTGTACAAGCATGCCACC+AGG | - | chr6_4:15958735-15958754 | None:intergenic | 45.0% |
| ! | ACTCCAGTTTTGCTACCGTA+CGG | + | chr6_4:15959076-15959095 | Msa0979300:intron | 45.0% |
| ! | TTTTGTACAAGCATGCCACC+AGG | - | chr6_4:15958735-15958754 | None:intergenic | 45.0% |
| ! | ACTCCAGTTTTGCTACCGTA+CGG | + | chr6_4:15959076-15959095 | Msa0979300:intron | 45.0% |
| !! | CAAAAATATAAATATAAATA+TGG | - | chr6_4:15959186-15959205 | None:intergenic | 5.0% |
| !! | CAAAAATATAAATATAAATA+TGG | - | chr6_4:15959186-15959205 | None:intergenic | 5.0% |
| ATCGAATGGGAACTTAGGCC+TGG | + | chr6_4:15958714-15958733 | Msa0979300:CDS | 50.0% | |
| ! | GTTTAGAGCCAAGAGAGCAG+AGG | + | chr6_4:15959350-15959369 | Msa0979300:CDS | 50.0% |
| TAGAGAAGCCTCTGCTCTCT+TGG | - | chr6_4:15959361-15959380 | None:intergenic | 50.0% | |
| GAGCAGAGGCTTCTCTACAA+AGG | + | chr6_4:15959364-15959383 | Msa0979300:CDS | 50.0% | |
| ATCGAATGGGAACTTAGGCC+TGG | + | chr6_4:15958714-15958733 | Msa0979300:CDS | 50.0% | |
| ! | GTTTAGAGCCAAGAGAGCAG+AGG | + | chr6_4:15959350-15959369 | Msa0979300:CDS | 50.0% |
| TAGAGAAGCCTCTGCTCTCT+TGG | - | chr6_4:15959361-15959380 | None:intergenic | 50.0% | |
| GAGCAGAGGCTTCTCTACAA+AGG | + | chr6_4:15959364-15959383 | Msa0979300:CDS | 50.0% | |
| GAATGGGAACTTAGGCCTGG+TGG | + | chr6_4:15958717-15958736 | Msa0979300:CDS | 55.0% | |
| ! | CCAGTTTTGCTACCGTACGG+CGG | + | chr6_4:15959079-15959098 | Msa0979300:intron | 55.0% |
| GAATGGGAACTTAGGCCTGG+TGG | + | chr6_4:15958717-15958736 | Msa0979300:CDS | 55.0% | |
| ! | CCAGTTTTGCTACCGTACGG+CGG | + | chr6_4:15959079-15959098 | Msa0979300:intron | 55.0% |
| CCGCCGTACGGTAGCAAAAC+TGG | - | chr6_4:15959082-15959101 | None:intergenic | 60.0% | |
| CCGCCGTACGGTAGCAAAAC+TGG | - | chr6_4:15959082-15959101 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 15958606 | 15959444 | 15958606 | ID=Msa0979300;Name=Msa0979300 |
| chr6_4 | mRNA | 15958606 | 15959444 | 15958606 | ID=Msa0979300-mRNA-1;Parent=Msa0979300;Name=Msa0979300-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|1|1|1|2|0|125 |
| chr6_4 | exon | 15958606 | 15958852 | 15958606 | ID=Msa0979300-mRNA-1:exon:5182;Parent=Msa0979300-mRNA-1 |
| chr6_4 | exon | 15959317 | 15959444 | 15959317 | ID=Msa0979300-mRNA-1:exon:5183;Parent=Msa0979300-mRNA-1 |
| chr6_4 | CDS | 15958606 | 15958852 | 15958606 | ID=Msa0979300-mRNA-1:cds;Parent=Msa0979300-mRNA-1 |
| chr6_4 | CDS | 15959317 | 15959444 | 15959317 | ID=Msa0979300-mRNA-1:cds;Parent=Msa0979300-mRNA-1 |
| Gene Sequence |
| Protein sequence |
