AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0980250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0980250 MtrunA17_Chr6g0460731 92.167 383 15 3 1 383 1 368 0.0 683
Msa0980250 MtrunA17_Chr6g0460721 73.569 367 59 6 1 367 1 329 0.0 520
Msa0980250 MtrunA17_Chr7g0254161 50.263 380 136 10 15 383 12 349 1.73e-112 335
Msa0980250 MtrunA17_Chr7g0217581 46.907 194 74 7 56 246 191 358 3.37e-42 155
Msa0980250 MtrunA17_Chr6g0453321 32.951 349 165 10 55 383 96 395 3.48e-42 154
Msa0980250 MtrunA17_Chr4g0042481 34.161 322 147 12 9 289 4 301 2.54e-41 151
Msa0980250 MtrunA17_Chr3g0092111 37.736 265 110 10 50 281 98 340 6.38e-41 150
Msa0980250 MtrunA17_Chr8g0355261 33.459 266 114 8 41 289 67 286 6.63e-37 139
Msa0980250 MtrunA17_Chr5g0417571 53.509 114 44 2 55 162 99 209 2.65e-34 129
Msa0980250 MtrunA17_Chr5g0417601 52.294 109 49 1 54 162 100 205 1.62e-30 120
Msa0980250 MtrunA17_Chr2g0283811 77.419 62 14 0 89 150 39 100 3.21e-30 114
Msa0980250 MtrunA17_Chr5g0417551 62.791 86 27 2 65 150 100 180 1.88e-29 117
Msa0980250 MtrunA17_Chr5g0417561 57.778 90 32 2 61 150 89 172 7.95e-29 115
Msa0980250 MtrunA17_Chr8g0367161 47.872 94 36 2 59 152 7 87 3.56e-24 102
Msa0980250 MtrunA17_Chr8g0346551 60.317 63 25 0 92 154 19 81 1.03e-23 99.4
Msa0980250 MtrunA17_Chr4g0047001 44.762 105 48 2 56 152 8 110 1.79e-23 100
Msa0980250 MtrunA17_Chr4g0010151 60.656 61 24 0 90 150 75 135 1.81e-23 100
Msa0980250 MtrunA17_Chr3g0127201 54.930 71 32 0 81 151 11 81 3.47e-23 98.2
Msa0980250 MtrunA17_Chr4g0030051 56.579 76 32 1 75 149 18 93 3.48e-23 98.2
Msa0980250 MtrunA17_Chr2g0326001 55.263 76 33 1 76 150 18 93 4.04e-23 98.2
Msa0980250 MtrunA17_Chr8g0374251 52.703 74 35 0 97 170 68 141 7.49e-23 98.2
Msa0980250 MtrunA17_Chr2g0328021 56.716 67 29 0 84 150 49 115 1.17e-22 96.7
Msa0980250 MtrunA17_Chr4g0031411 61.667 60 23 0 90 149 68 127 1.65e-22 97.1
Msa0980250 MtrunA17_Chr8g0374111 42.623 122 63 3 97 218 29 143 2.02e-22 94.7
Msa0980250 MtrunA17_Chr2g0282051 67.241 58 18 1 95 152 44 100 1.17e-21 93.6
Msa0980250 MtrunA17_Chr2g0308011 69.388 49 15 0 97 145 37 85 1.43e-21 92.0
Msa0980250 MtrunA17_Chr2g0282441 54.688 64 29 0 85 148 13 76 2.02e-21 94.0
Msa0980250 MtrunA17_Chr1g0188851 47.500 80 37 1 75 149 16 95 2.22e-21 93.2
Msa0980250 MtrunA17_Chr7g0237921 53.521 71 31 1 82 150 59 129 2.28e-21 94.0
Msa0980250 MtrunA17_Chr4g0047761 50.549 91 29 2 77 152 37 126 2.63e-21 93.6
Msa0980250 MtrunA17_Chr8g0341671 65.385 52 18 0 97 148 20 71 2.74e-21 91.3
Msa0980250 MtrunA17_Chr4g0061761 64.151 53 19 0 95 147 38 90 4.58e-21 92.8
Msa0980250 MtrunA17_Chr7g0223311 67.308 52 17 0 98 149 78 129 5.61e-21 93.2
Msa0980250 MtrunA17_Chr3g0126201 54.167 72 31 1 81 150 33 104 2.28e-20 90.9
Msa0980250 MtrunA17_Chr5g0411951 60.000 55 22 0 93 147 39 93 2.61e-20 90.9
Msa0980250 MtrunA17_Chr2g0282511 67.347 49 16 0 97 145 45 93 6.31e-20 89.4
Msa0980250 MtrunA17_Chr3g0117601 56.452 62 26 1 87 147 50 111 1.07e-19 89.4
Msa0980250 MtrunA17_Chr1g0177251 65.385 52 18 0 99 150 14 65 1.27e-19 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0980250 AT3G47500.1 56.667 120 46 3 53 172 71 184 3.02e-39 145
Msa0980250 AT5G39660.3 44.828 174 61 6 63 221 92 245 9.56e-36 136
Msa0980250 AT5G39660.1 44.828 174 61 6 63 221 92 245 9.56e-36 136
Msa0980250 AT5G39660.2 44.828 174 61 6 63 221 92 245 9.56e-36 136
Msa0980250 AT5G62430.1 52.137 117 49 2 64 178 26 137 6.78e-35 130
Msa0980250 AT2G34140.1 58.065 93 26 1 71 150 19 111 1.00e-33 124
Msa0980250 AT1G26790.1 48.951 143 60 4 55 184 67 209 1.15e-33 129
Msa0980250 AT1G69570.1 45.385 130 43 2 85 213 120 222 3.45e-31 122
Msa0980250 AT1G29160.1 78.333 60 13 0 91 150 56 115 3.87e-31 117
Msa0980250 AT3G55370.1 57.143 70 30 0 93 162 72 141 1.94e-24 102
Msa0980250 AT3G55370.2 57.143 70 30 0 93 162 72 141 2.23e-24 103
Msa0980250 AT3G55370.3 57.143 70 30 0 93 162 117 186 4.69e-24 102
Msa0980250 AT2G28810.2 40.000 100 57 1 52 148 29 128 5.11e-23 99.0
Msa0980250 AT2G28810.1 40.000 100 57 1 52 148 46 145 5.85e-23 99.0
Msa0980250 AT5G60200.1 59.375 64 26 0 91 154 49 112 7.62e-23 97.1
Msa0980250 AT3G21270.1 69.811 53 16 0 97 149 29 81 1.12e-22 95.1
Msa0980250 AT2G37590.1 62.069 58 22 0 93 150 85 142 1.79e-22 97.4
Msa0980250 AT3G45610.1 45.055 91 42 2 93 175 36 126 2.37e-22 95.1
Msa0980250 AT1G51700.1 52.857 70 31 1 79 148 17 84 4.14e-22 93.2
Msa0980250 AT1G28310.1 64.286 56 20 0 97 152 27 82 1.34e-21 94.7
Msa0980250 AT3G61850.2 61.017 59 23 0 92 150 57 115 1.35e-21 94.0
Msa0980250 AT3G61850.3 61.017 59 23 0 92 150 57 115 1.35e-21 94.0
Msa0980250 AT4G24060.1 56.923 65 28 0 86 150 42 106 1.47e-21 95.1
Msa0980250 AT1G28310.2 64.286 56 20 0 97 152 51 106 1.48e-21 94.7
Msa0980250 AT3G61850.1 61.017 59 23 0 92 150 69 127 1.82e-21 94.0
Msa0980250 AT5G60850.1 66.667 54 18 0 97 150 51 104 1.94e-21 94.0
Msa0980250 AT3G61850.5 61.017 59 23 0 92 150 57 115 2.46e-21 94.0
Msa0980250 AT1G07640.1 66.038 53 18 0 97 149 21 73 2.55e-21 93.2
Msa0980250 AT3G50410.1 66.038 53 18 0 97 149 30 82 2.76e-21 92.4
Msa0980250 AT2G28510.1 66.071 56 19 0 99 154 50 105 2.84e-21 93.2
Msa0980250 AT3G61850.4 61.017 59 23 0 92 150 69 127 2.89e-21 94.0
Msa0980250 AT1G07640.2 66.038 53 18 0 97 149 77 129 3.86e-21 93.6
Msa0980250 AT5G02460.1 59.016 61 25 0 90 150 88 148 4.51e-21 94.4
Msa0980250 AT5G66940.1 53.425 73 27 1 84 156 26 91 4.70e-21 91.3
Msa0980250 AT1G07640.3 66.038 53 18 0 97 149 85 137 5.24e-21 93.2
Msa0980250 AT5G65590.1 64.815 54 19 0 97 150 41 94 6.41e-21 92.8
Msa0980250 AT4G00940.1 52.778 72 30 1 85 152 52 123 1.96e-20 90.9
Msa0980250 AT4G00940.3 52.778 72 30 1 85 152 52 123 1.96e-20 90.9
Msa0980250 AT4G00940.2 52.778 72 30 1 85 152 52 123 1.96e-20 90.9
Msa0980250 AT1G21340.1 42.553 94 53 1 99 191 40 133 4.94e-20 89.4
Msa0980250 AT5G62940.1 39.450 109 50 2 55 147 15 123 7.21e-20 90.5
Msa0980250 AT4G21050.1 47.436 78 39 1 77 152 2 79 7.99e-20 87.4
Msa0980250 AT4G38000.1 49.367 79 38 2 96 172 40 118 1.01e-19 87.8
Msa0980250 AT3G52440.1 63.462 52 19 0 99 150 27 78 2.13e-19 87.0
Msa0980250 AT1G64620.1 53.333 60 28 0 86 145 38 97 2.53e-19 88.6
Msa0980250 AT1G47655.1 65.306 49 17 0 97 145 29 77 3.40e-19 85.5
Msa0980250 AT3G52440.2 63.462 52 19 0 99 150 46 97 3.43e-19 87.0
Msa0980250 AT2G46590.1 58.491 53 22 0 92 144 63 115 1.32e-18 86.7
Msa0980250 AT2G46590.2 58.491 53 22 0 92 144 75 127 1.55e-18 86.7
Msa0980250 AT4G21040.1 48.611 72 37 0 99 170 27 98 9.42e-16 76.3
Msa0980250 AT4G21080.1 50.877 57 28 0 99 155 27 83 1.10e-14 73.6

Find 88 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATTAACGAAGCAGATTT+TGG 0.193943 6_4:-18195981 Msa0980250:three_prime_UTR
AATGGTATTGTCCTAAAATT+TGG 0.197738 6_4:-18196605 Msa0980250:CDS
TAGAAACCAACGACTCTTCT+AGG 0.239536 6_4:-18196682 Msa0980250:CDS
TTGGAACAATGTTACTTCTA+TGG 0.251370 6_4:-18196339 Msa0980250:CDS
GGTAACAATTGTAGAGAAAA+TGG 0.277928 6_4:-18196503 Msa0980250:CDS
GGTGAGTTCTTCTCAGATTC+AGG 0.283209 6_4:-18198052 Msa0980250:CDS
AAGATTAAGCATTGAACCTT+TGG 0.298547 6_4:+18196556 None:intergenic
TCTTCTAGTTTCATGCTCTT+TGG 0.299226 6_4:-18198087 Msa0980250:CDS
TTTAGGTGTTCAAATGATAA+TGG 0.318424 6_4:-18196623 Msa0980250:CDS
GTCGGAGTTGTCACAGATAT+TGG 0.320960 6_4:-18196823 Msa0980250:CDS
TAATTCACTTCTATAGAGTT+TGG 0.326559 6_4:+18195928 None:intergenic
TTGTTCCAACTTTGATTCAT+TGG 0.340788 6_4:+18196353 None:intergenic
ATTTACCATGGTTGTAGAAT+TGG 0.341653 6_4:+18198029 None:intergenic
AAGCACTGTGGAAGATAATA+TGG 0.348391 6_4:-18196978 Msa0980250:CDS
CCTTTGTATGGTAGTGGAAA+TGG 0.370217 6_4:-18196170 Msa0980250:CDS
ATGCAGATGTGTAACCCTTA+TGG 0.380909 6_4:-18196305 Msa0980250:CDS
AGAGTCGTTGGTTTCTAAAC+TGG 0.381811 6_4:+18196688 None:intergenic
CTCTATAGAAGTGAATTATA+AGG 0.389064 6_4:-18195923 Msa0980250:three_prime_UTR
GGACATTGAACAATCTTGTC+TGG 0.411323 6_4:+18196917 None:intergenic
ACAAATTGCAAGCCAATGTT+TGG 0.418330 6_4:+18196218 None:intergenic
GCTCTTTGGTAAGAAGATTC+CGG 0.421687 6_4:-18198073 Msa0980250:CDS
TGCAGTAACTCAAAGCATAA+AGG 0.422749 6_4:-18197079 Msa0980250:CDS
AGGTTTAGACCTATTCTGTT+CGG 0.445629 6_4:+18196409 None:intergenic
GTGTCACGCGCCGAGGGTTG+CGG 0.447216 6_4:-18196258 Msa0980250:CDS
AGAGCTACCCTGCACCTTCA+TGG 0.447832 6_4:-18196382 Msa0980250:CDS
ACTAGTAATAGTTGTATCTC+AGG 0.449615 6_4:-18196098 Msa0980250:CDS
AATTTGTTCCTGCATCAAAC+AGG 0.461385 6_4:-18196202 Msa0980250:CDS
CAATCTTGTCTGGTTTCTTG+AGG 0.465489 6_4:+18196927 None:intergenic
AGAAATGTTGCTGTCGGTGC+AGG 0.467099 6_4:-18196782 Msa0980250:CDS
GTTCCATTCCTGTTTGATGC+AGG 0.467432 6_4:+18196194 None:intergenic
ATTTCTCATTGTTCCACCAT+TGG 0.468075 6_4:+18196799 None:intergenic
TTCATTGGAATTATCCATGA+AGG 0.472286 6_4:+18196368 None:intergenic
TTGCATCAACATTTCTCTTG+TGG 0.474797 6_4:+18196528 None:intergenic
AATGCAGCCATTTGAACCTA+TGG 0.483789 6_4:+18196139 None:intergenic
TATTGTCCTAAAATTTGGTC+GGG 0.484036 6_4:-18196600 Msa0980250:CDS
TATTCGAATCCGAACAGAAT+AGG 0.487381 6_4:-18196418 Msa0980250:CDS
GAAGTAGGATCAATGCCATA+AGG 0.489322 6_4:+18196290 None:intergenic
TATAGAAAGAGAAAAGTGTT+TGG 0.490803 6_4:+18198242 None:intergenic
GGAATTATCCATGAAGGTGC+AGG 0.491213 6_4:+18196374 None:intergenic
CATGTGTGTTTGCCAAGGAT+TGG 0.493826 6_4:+18196065 None:intergenic
AATAGTTGTATCTCAGGTAA+TGG 0.494093 6_4:-18196092 Msa0980250:CDS
GGAACACCTTTGTATGGTAG+TGG 0.497692 6_4:-18196176 Msa0980250:CDS
TTGCTGTCGGTGCAGGGAGA+CGG 0.499466 6_4:-18196775 Msa0980250:CDS
TAATTCCAATGAATCAAAGT+TGG 0.500347 6_4:-18196358 Msa0980250:CDS
GCAGTGGTGTCACGCGCCGA+GGG 0.511659 6_4:-18196264 Msa0980250:CDS
ATCTGCTTCGTTAATTGTAA+CGG 0.514386 6_4:+18195986 None:intergenic
GGTAATGGCACACCAATCCT+TGG 0.524374 6_4:-18196077 Msa0980250:CDS
AGGAATGGAACACCTTTGTA+TGG 0.533609 6_4:-18196182 Msa0980250:CDS
TTGGTACCTAGAAGAGTCGT+TGG 0.535519 6_4:+18196676 None:intergenic
CACAGATATTGGACCAATGG+TGG 0.546696 6_4:-18196812 Msa0980250:CDS
GTATTGTCCTAAAATTTGGT+CGG 0.547769 6_4:-18196601 Msa0980250:CDS
ATTCAACATTTGAGCCATGT+TGG 0.548791 6_4:+18196657 None:intergenic
GTTCCTGCATCAAACAGGAA+TGG 0.554691 6_4:-18196197 Msa0980250:CDS
AAGAGAAATGTTGATGCAAG+TGG 0.555436 6_4:-18196524 Msa0980250:CDS
TGTGTATCGTCTGTGACAAA+CGG 0.557160 6_4:-18196461 Msa0980250:CDS
ACCTTTGGATTCATCGAGAG+AGG 0.559535 6_4:+18196571 None:intergenic
AAGTAGGATCAATGCCATAA+GGG 0.561604 6_4:+18196291 None:intergenic
AATGTGTCCATAGGTTCAAA+TGG 0.563336 6_4:-18196146 Msa0980250:CDS
GGAAATGGAAATGTGTCCAT+AGG 0.578755 6_4:-18196155 Msa0980250:CDS
ATTGCGACAATATATTTACA+AGG 0.579772 6_4:+18195886 None:intergenic
TGTCACAGATATTGGACCAA+TGG 0.583718 6_4:-18196815 Msa0980250:CDS
TTGGTTTCTAAACTGGCGGT+CGG 0.586129 6_4:+18196695 None:intergenic
AATCTCATGTGTGTTTGCCA+AGG 0.586652 6_4:+18196060 None:intergenic
TGACACCACTGCATTGAAGT+AGG 0.587068 6_4:+18196275 None:intergenic
ATCAACCTCGGCATTTCTGT+CGG 0.588083 6_4:-18196841 Msa0980250:CDS
ATGTTTGTAACCGCAACCCT+CGG 0.590311 6_4:+18196248 None:intergenic
TCAGGCCAATTCTACAACCA+TGG 0.595350 6_4:-18198034 Msa0980250:intron
GGTGCAGGGTAGCTCTGCAT+AGG 0.600830 6_4:+18196389 None:intergenic
CACATGATTGTAGTGTCCGA+TGG 0.602575 6_4:-18196722 Msa0980250:CDS
GTGACAAACGGTCATACTCG+TGG 0.605386 6_4:-18196449 Msa0980250:CDS
GTCGTTGGTTTCTAAACTGG+CGG 0.606694 6_4:+18196691 None:intergenic
CCATTTCCACTACCATACAA+AGG 0.611279 6_4:+18196170 None:intergenic
CTCAAATGTTGAATCTGCTG+CGG 0.611992 6_4:-18196648 Msa0980250:CDS
GCCTCTCTCGATGAATCCAA+AGG 0.612065 6_4:-18196572 Msa0980250:CDS
TTACTAGTAGTAGATGAAGA+AGG 0.612171 6_4:+18196113 None:intergenic
TGAATCTGAGAAGAACTCAC+CGG 0.630634 6_4:+18198054 None:intergenic
GAATTATCCATGAAGGTGCA+GGG 0.632904 6_4:+18196375 None:intergenic
ACTGGCGGTCGGAATTCCAT+CGG 0.649455 6_4:+18196706 None:intergenic
TTGATCCTACTTCAATGCAG+TGG 0.649462 6_4:-18196280 Msa0980250:CDS
ATATGTGAGCATTTGTACGA+AGG 0.659474 6_4:-18196010 Msa0980250:three_prime_UTR
GAAATGTTGCTGTCGGTGCA+GGG 0.663844 6_4:-18196781 Msa0980250:CDS
CAACTCCGACAGAAATGCCG+AGG 0.680790 6_4:+18196836 None:intergenic
ACTACAACGTCAATCAACCT+CGG 0.682406 6_4:-18196853 Msa0980250:CDS
GACTCTTCTAGGTACCAACA+TGG 0.687547 6_4:-18196671 Msa0980250:CDS
ACAATGAGAAATGTTGCTGT+CGG 0.691886 6_4:-18196788 Msa0980250:CDS
CATCGGACACTACAATCATG+TGG 0.695881 6_4:+18196723 None:intergenic
CAATTGTAGAGAAAATGGAG+AGG 0.705347 6_4:-18196498 Msa0980250:CDS
AAAAGTGAACACAAGCACTG+TGG 0.727270 6_4:-18196990 Msa0980250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTATTTTTGTTGTAT+TGG - chr6_4:18196250-18196269 Msa0980250:CDS 10.0%
!!! TGATTTTTTTATAAGAAAAT+TGG - chr6_4:18196541-18196560 Msa0980250:CDS 10.0%
!!! ACATTAAATTTGTTTAATTT+TGG - chr6_4:18196635-18196654 Msa0980250:CDS 10.0%
!! TAAAAAAACAACATAAACAA+TGG + chr6_4:18196211-18196230 None:intergenic 15.0%
!!! ATTTTAAATGTTTCAAAGAT+TGG + chr6_4:18196292-18196311 None:intergenic 15.0%
!!! TAAATTTGTTTAATTTTGGT+TGG - chr6_4:18196639-18196658 Msa0980250:CDS 15.0%
!! TTTCATAAATATAACATGAT+TGG - chr6_4:18196719-18196738 Msa0980250:CDS 15.0%
!!! TAATTTTGAAAAGTCTTTGT+AGG - chr6_4:18195849-18195868 Msa0980250:three_prime_UTR 20.0%
!!! AATTTTGAAAAGTCTTTGTA+GGG - chr6_4:18195850-18195869 Msa0980250:three_prime_UTR 20.0%
!! AACAATATCACAAAAATGAA+TGG - chr6_4:18196046-18196065 Msa0980250:exon 20.0%
!! CATAAAAAAAATCAAGAAGA+TGG - chr6_4:18196355-18196374 Msa0980250:CDS 20.0%
!! ATGTCAATACATACATATAT+AGG - chr6_4:18196466-18196485 Msa0980250:CDS 20.0%
!! AATTTAAGAAATTGAGTCTA+AGG - chr6_4:18196491-18196510 Msa0980250:CDS 20.0%
!! ATTTGACAGAACTTTTCAAA+GGG + chr6_4:18195897-18195916 None:intergenic 25.0%
!! AATTTGACAGAACTTTTCAA+AGG + chr6_4:18195898-18195917 None:intergenic 25.0%
!!! TATAGAAAGAGAAAAGTGTT+TGG + chr6_4:18195942-18195961 None:intergenic 25.0%
! GATAAAATGAGTATTTACCA+TGG + chr6_4:18196167-18196186 None:intergenic 25.0%
! CAACATAAACAATGGTTATT+GGG + chr6_4:18196203-18196222 None:intergenic 25.0%
! ACAACATAAACAATGGTTAT+TGG + chr6_4:18196204-18196223 None:intergenic 25.0%
! GATCTGATTCATTTAAACAA+TGG - chr6_4:18196377-18196396 Msa0980250:CDS 25.0%
!!! CAGATTAGTTTTAGTAACAT+AGG - chr6_4:18196774-18196793 Msa0980250:CDS 25.0%
! AAAAACCATAAAATTTGACG+TGG + chr6_4:18196966-18196985 None:intergenic 25.0%
! AAGTCATGAAGAATAAAGTT+TGG + chr6_4:18197046-18197065 None:intergenic 25.0%
! TTTAGGTGTTCAAATGATAA+TGG - chr6_4:18197558-18197577 Msa0980250:intron 25.0%
! AATGGTATTGTCCTAAAATT+TGG - chr6_4:18197576-18197595 Msa0980250:intron 25.0%
! TAATTCCAATGAATCAAAGT+TGG - chr6_4:18197823-18197842 Msa0980250:intron 25.0%
! TAATTCACTTCTATAGAGTT+TGG + chr6_4:18198256-18198275 None:intergenic 25.0%
! CTCTATAGAAGTGAATTATA+AGG - chr6_4:18198258-18198277 Msa0980250:five_prime_UTR 25.0%
! ATTGCGACAATATATTTACA+AGG + chr6_4:18198298-18198317 None:intergenic 25.0%
ATCACAAAAATGAATGGTGA+AGG - chr6_4:18196052-18196071 Msa0980250:exon 30.0%
ATTTACCATGGTTGTAGAAT+TGG + chr6_4:18196155-18196174 None:intergenic 30.0%
TGATTCATTTAAACAATGGC+TGG - chr6_4:18196381-18196400 Msa0980250:CDS 30.0%
! ATGTACCACGTCAAATTTTA+TGG - chr6_4:18196958-18196977 Msa0980250:CDS 30.0%
!! TTCTCAATGTTTTGAGTATG+AGG + chr6_4:18197138-18197157 None:intergenic 30.0%
GTATTGTCCTAAAATTTGGT+CGG - chr6_4:18197580-18197599 Msa0980250:intron 30.0%
TATTGTCCTAAAATTTGGTC+GGG - chr6_4:18197581-18197600 Msa0980250:intron 30.0%
!! AAGATTAAGCATTGAACCTT+TGG + chr6_4:18197628-18197647 None:intergenic 30.0%
GGTAACAATTGTAGAGAAAA+TGG - chr6_4:18197678-18197697 Msa0980250:intron 30.0%
TTCATTGGAATTATCCATGA+AGG + chr6_4:18197816-18197835 None:intergenic 30.0%
TTGTTCCAACTTTGATTCAT+TGG + chr6_4:18197831-18197850 None:intergenic 30.0%
! TTGGAACAATGTTACTTCTA+TGG - chr6_4:18197842-18197861 Msa0980250:intron 30.0%
! AACATTTTCACACCAAACAT+TGG - chr6_4:18197951-18197970 Msa0980250:intron 30.0%
TTACTAGTAGTAGATGAAGA+AGG + chr6_4:18198071-18198090 None:intergenic 30.0%
ACTAGTAATAGTTGTATCTC+AGG - chr6_4:18198083-18198102 Msa0980250:CDS 30.0%
AATAGTTGTATCTCAGGTAA+TGG - chr6_4:18198089-18198108 Msa0980250:CDS 30.0%
ATCTGCTTCGTTAATTGTAA+CGG + chr6_4:18198198-18198217 None:intergenic 30.0%
! ACAATTAACGAAGCAGATTT+TGG - chr6_4:18198200-18198219 Msa0980250:five_prime_UTR 30.0%
!! TCTTCTAGTTTCATGCTCTT+TGG - chr6_4:18196094-18196113 Msa0980250:CDS 35.0%
CGTTGAGTTTGACAAAATCA+CGG - chr6_4:18196826-18196845 Msa0980250:CDS 35.0%
TGCAGTAACTCAAAGCATAA+AGG - chr6_4:18197102-18197121 Msa0980250:intron 35.0%
! AAGCACTGTGGAAGATAATA+TGG - chr6_4:18197203-18197222 Msa0980250:intron 35.0%
! GTGTCGAAACTTTTACATCT+CGG + chr6_4:18197288-18197307 None:intergenic 35.0%
ATTTCTCATTGTTCCACCAT+TGG + chr6_4:18197385-18197404 None:intergenic 35.0%
ACAATGAGAAATGTTGCTGT+CGG - chr6_4:18197393-18197412 Msa0980250:intron 35.0%
ATTCAACATTTGAGCCATGT+TGG + chr6_4:18197527-18197546 None:intergenic 35.0%
! CATTTTCCCGACCAAATTTT+AGG + chr6_4:18197590-18197609 None:intergenic 35.0%
TTGCATCAACATTTCTCTTG+TGG + chr6_4:18197656-18197675 None:intergenic 35.0%
AAGAGAAATGTTGATGCAAG+TGG - chr6_4:18197657-18197676 Msa0980250:intron 35.0%
CAATTGTAGAGAAAATGGAG+AGG - chr6_4:18197683-18197702 Msa0980250:intron 35.0%
TATTCGAATCCGAACAGAAT+AGG - chr6_4:18197763-18197782 Msa0980250:intron 35.0%
AGGTTTAGACCTATTCTGTT+CGG + chr6_4:18197775-18197794 None:intergenic 35.0%
AAGTAGGATCAATGCCATAA+GGG + chr6_4:18197893-18197912 None:intergenic 35.0%
ACAAATTGCAAGCCAATGTT+TGG + chr6_4:18197966-18197985 None:intergenic 35.0%
! AATTTGTTCCTGCATCAAAC+AGG - chr6_4:18197979-18197998 Msa0980250:intron 35.0%
AATGTGTCCATAGGTTCAAA+TGG - chr6_4:18198035-18198054 Msa0980250:CDS 35.0%
ATATGTGAGCATTTGTACGA+AGG - chr6_4:18198171-18198190 Msa0980250:five_prime_UTR 35.0%
GCTCTTTGGTAAGAAGATTC+CGG - chr6_4:18196108-18196127 Msa0980250:CDS 40.0%
TGAATCTGAGAAGAACTCAC+CGG + chr6_4:18196130-18196149 None:intergenic 40.0%
! GATTTTGTCAAACTCAACGC+TGG + chr6_4:18196825-18196844 None:intergenic 40.0%
! AAAAGTGAACACAAGCACTG+TGG - chr6_4:18197191-18197210 Msa0980250:intron 40.0%
! CAATCTTGTCTGGTTTCTTG+AGG + chr6_4:18197257-18197276 None:intergenic 40.0%
GGACATTGAACAATCTTGTC+TGG + chr6_4:18197267-18197286 None:intergenic 40.0%
ACTACAACGTCAATCAACCT+CGG - chr6_4:18197328-18197347 Msa0980250:intron 40.0%
TGTCACAGATATTGGACCAA+TGG - chr6_4:18197366-18197385 Msa0980250:intron 40.0%
!! AGAGTCGTTGGTTTCTAAAC+TGG + chr6_4:18197496-18197515 None:intergenic 40.0%
TAGAAACCAACGACTCTTCT+AGG - chr6_4:18197499-18197518 Msa0980250:intron 40.0%
CTCAAATGTTGAATCTGCTG+CGG - chr6_4:18197533-18197552 Msa0980250:intron 40.0%
! TTGAATCTGCTGCGGTTTTT+AGG - chr6_4:18197541-18197560 Msa0980250:intron 40.0%
TGTGTATCGTCTGTGACAAA+CGG - chr6_4:18197720-18197739 Msa0980250:intron 40.0%
! GAATTATCCATGAAGGTGCA+GGG + chr6_4:18197809-18197828 None:intergenic 40.0%
ATGCAGATGTGTAACCCTTA+TGG - chr6_4:18197876-18197895 Msa0980250:intron 40.0%
GAAGTAGGATCAATGCCATA+AGG + chr6_4:18197894-18197913 None:intergenic 40.0%
TTGATCCTACTTCAATGCAG+TGG - chr6_4:18197901-18197920 Msa0980250:intron 40.0%
AGGAATGGAACACCTTTGTA+TGG - chr6_4:18197999-18198018 Msa0980250:intron 40.0%
CCATTTCCACTACCATACAA+AGG + chr6_4:18198014-18198033 None:intergenic 40.0%
!! CCTTTGTATGGTAGTGGAAA+TGG - chr6_4:18198011-18198030 Msa0980250:intron 40.0%
GGAAATGGAAATGTGTCCAT+AGG - chr6_4:18198026-18198045 Msa0980250:intron 40.0%
AATGCAGCCATTTGAACCTA+TGG + chr6_4:18198045-18198064 None:intergenic 40.0%
AATCTCATGTGTGTTTGCCA+AGG + chr6_4:18198124-18198143 None:intergenic 40.0%
GGTGAGTTCTTCTCAGATTC+AGG - chr6_4:18196129-18196148 Msa0980250:CDS 45.0%
TCAGGCCAATTCTACAACCA+TGG - chr6_4:18196147-18196166 Msa0980250:CDS 45.0%
CTGGTAAGTCCATAAACCAG+GGG + chr6_4:18196806-18196825 None:intergenic 45.0%
GCTGGTAAGTCCATAAACCA+GGG + chr6_4:18196807-18196826 None:intergenic 45.0%
ATCAACCTCGGCATTTCTGT+CGG - chr6_4:18197340-18197359 Msa0980250:intron 45.0%
GTCGGAGTTGTCACAGATAT+TGG - chr6_4:18197358-18197377 Msa0980250:intron 45.0%
CACAGATATTGGACCAATGG+TGG - chr6_4:18197369-18197388 Msa0980250:intron 45.0%
CATCGGACACTACAATCATG+TGG + chr6_4:18197461-18197480 None:intergenic 45.0%
CACATGATTGTAGTGTCCGA+TGG - chr6_4:18197459-18197478 Msa0980250:intron 45.0%
TTGGTTTCTAAACTGGCGGT+CGG + chr6_4:18197489-18197508 None:intergenic 45.0%
!! GTCGTTGGTTTCTAAACTGG+CGG + chr6_4:18197493-18197512 None:intergenic 45.0%
TTGGTACCTAGAAGAGTCGT+TGG + chr6_4:18197508-18197527 None:intergenic 45.0%
GACTCTTCTAGGTACCAACA+TGG - chr6_4:18197510-18197529 Msa0980250:intron 45.0%
ACCTTTGGATTCATCGAGAG+AGG + chr6_4:18197613-18197632 None:intergenic 45.0%
! GGAATTATCCATGAAGGTGC+AGG + chr6_4:18197810-18197829 None:intergenic 45.0%
TGACACCACTGCATTGAAGT+AGG + chr6_4:18197909-18197928 None:intergenic 45.0%
ATGTTTGTAACCGCAACCCT+CGG + chr6_4:18197936-18197955 None:intergenic 45.0%
GTTCCTGCATCAAACAGGAA+TGG - chr6_4:18197984-18198003 Msa0980250:intron 45.0%
GTTCCATTCCTGTTTGATGC+AGG + chr6_4:18197990-18198009 None:intergenic 45.0%
GGAACACCTTTGTATGGTAG+TGG - chr6_4:18198005-18198024 Msa0980250:intron 45.0%
CATGTGTGTTTGCCAAGGAT+TGG + chr6_4:18198119-18198138 None:intergenic 45.0%
GTAACATAGGAGATAGCCCC+TGG - chr6_4:18196787-18196806 Msa0980250:CDS 50.0%
AGGAGATAGCCCCTGGTTTA+TGG - chr6_4:18196794-18196813 Msa0980250:CDS 50.0%
CGCTGGTAAGTCCATAAACC+AGG + chr6_4:18196808-18196827 None:intergenic 50.0%
AGAAATGTTGCTGTCGGTGC+AGG - chr6_4:18197399-18197418 Msa0980250:intron 50.0%
GAAATGTTGCTGTCGGTGCA+GGG - chr6_4:18197400-18197419 Msa0980250:intron 50.0%
GCCTCTCTCGATGAATCCAA+AGG - chr6_4:18197609-18197628 Msa0980250:intron 50.0%
GTGACAAACGGTCATACTCG+TGG - chr6_4:18197732-18197751 Msa0980250:intron 50.0%
! GGTAATGGCACACCAATCCT+TGG - chr6_4:18198104-18198123 Msa0980250:CDS 50.0%
CAACTCCGACAGAAATGCCG+AGG + chr6_4:18197348-18197367 None:intergenic 55.0%
ACTGGCGGTCGGAATTCCAT+CGG + chr6_4:18197478-18197497 None:intergenic 55.0%
AGAGCTACCCTGCACCTTCA+TGG - chr6_4:18197799-18197818 Msa0980250:intron 55.0%
TTGCTGTCGGTGCAGGGAGA+CGG - chr6_4:18197406-18197425 Msa0980250:intron 60.0%
! GGTGCAGGGTAGCTCTGCAT+AGG + chr6_4:18197795-18197814 None:intergenic 60.0%
!! TGCAGTGGTGTCACGCGCCG+AGG - chr6_4:18197916-18197935 Msa0980250:intron 70.0%
!! GCAGTGGTGTCACGCGCCGA+GGG - chr6_4:18197917-18197936 Msa0980250:intron 70.0%
GTGTCACGCGCCGAGGGTTG+CGG - chr6_4:18197923-18197942 Msa0980250:intron 70.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 18195848 18198355 18195848 ID=Msa0980250;Name=Msa0980250
chr6_4 mRNA 18195848 18198355 18195848 ID=Msa0980250-mRNA-1;Parent=Msa0980250;Name=Msa0980250-mRNA-1;_AED=0.00;_eAED=0.00;_QI=213|1|1|1|1|1|2|216|383
chr6_4 exon 18195848 18197107 18195848 ID=Msa0980250-mRNA-1:exon:5641;Parent=Msa0980250-mRNA-1
chr6_4 exon 18198035 18198355 18198035 ID=Msa0980250-mRNA-1:exon:5640;Parent=Msa0980250-mRNA-1
chr6_4 five_prime_UTR 18198143 18198355 18198143 ID=Msa0980250-mRNA-1:five_prime_utr;Parent=Msa0980250-mRNA-1
chr6_4 CDS 18198035 18198142 18198035 ID=Msa0980250-mRNA-1:cds;Parent=Msa0980250-mRNA-1
chr6_4 CDS 18196064 18197107 18196064 ID=Msa0980250-mRNA-1:cds;Parent=Msa0980250-mRNA-1
chr6_4 three_prime_UTR 18195848 18196063 18195848 ID=Msa0980250-mRNA-1:three_prime_utr;Parent=Msa0980250-mRNA-1
Gene Sequence

>Msa0980250

ATGAATGGTGAAGGTGACATGTTTGTTAAAGATTCTTCTAGTTTCATGCTCTTTGGTAAGAAGATTCCGGTGAGTTCTTCTCAGATTCAGGCCAATTCTACAACCATGATTTCTTGCAGTAACTCAAAGCATAAAGGAAATGATATGCCTCATACTCAAAACATTGAGAAATCAAAGAATTCTTCTAATGAAGAAAATGAATCAAAAGTGAACACAAGCACTGTGGAAGATAATATGGAAACTAGCAGCACAGATCAAGACAAAGTCCTCAAGAAACCAGACAAGATTGTTCAATGTCCGAGATGTAAAAGTTTCGACACAAAATTTTGTTATTTCAACAACTACAACGTCAATCAACCTCGGCATTTCTGTCGGAGTTGTCACAGATATTGGACCAATGGTGGAACAATGAGAAATGTTGCTGTCGGTGCAGGGAGACGGAAGAACAAACACATAGCTTCTCAATATCGCCACATGATTGTAGTGTCCGATGGAATTCCGACCGCCAGTTTAGAAACCAACGACTCTTCTAGGTACCAACATGGCTCAAATGTTGAATCTGCTGCGGTTTTTAGGTGTTCAAATGATAATGGTATTGTCCTAAAATTTGGTCGGGAAAATGCCTCTCTCGATGAATCCAAAGGTTCAATGCTTAATCTTATGAACCACAAGAGAAATGTTGATGCAAGTGGTAACAATTGTAGAGAAAATGGAGAGGAAGAATCATCGTTATGTGTATCGTCTGTGACAAACGGTCATACTCGTGGAAACGAGTTATTCGAATCCGAACAGAATAGGTCTAAACCTATGCAGAGCTACCCTGCACCTTCATGGATAATTCCAATGAATCAAAGTTGGAACAATGTTACTTCTATGGTTCAATCATCAATGCAGATGTGTAACCCTTATGGCATTGATCCTACTTCAATGCAGTGGTGTCACGCGCCGAGGGTTGCGGTTACAAACATTTTCACACCAAACATTGGCTTGCAATTTGTTCCTGCATCAAACAGGAATGGAACACCTTTGTATGGTAGTGGAAATGGAAATGTGTCCATAGGTTCAAATGGCTGCATTTCACCTTCTTCATCTACTACTAGTAATAGTTGTATCTCAGGTAATGGCACACCAATCCTTGGCAAACACACATGA
Protein sequence

>Msa0980250

MNGEGDMFVKDSSSFMLFGKKIPVSSSQIQANSTTMISCSNSKHKGNDMPHTQNIEKSKNSSNEENESKVNTSTVEDNMETSSTDQDKVLKKPDKIVQCPRCKSFDTKFCYFNNYNVNQPRHFCRSCHRYWTNGGTMRNVAVGAGRRKNKHIASQYRHMIVVSDGIPTASLETNDSSRYQHGSNVESAAVFRCSNDNGIVLKFGRENASLDESKGSMLNLMNHKRNVDASGNNCRENGEEESSLCVSSVTNGHTRGNELFESEQNRSKPMQSYPAPSWIIPMNQSWNNVTSMVQSSMQMCNPYGIDPTSMQWCHAPRVAVTNIFTPNIGLQFVPASNRNGTPLYGSGNGNVSIGSNGCISPSSSTTSNSCISGNGTPILGKHT*