AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0980450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0980450 MtrunA17_Chr6g0460881 89.510 572 22 1 172 705 57 628 0.0 1013
Msa0980450 MtrunA17_Chr6g0460881 83.636 55 6 3 1 52 1 55 7.45e-18 87.8
Msa0980450 MtrunA17_Chr6g0460891 85.062 569 48 1 172 703 50 618 0.0 960
Msa0980450 MtrunA17_Chr6g0460891 84.615 52 4 1 1 52 1 48 2.77e-19 92.4
Msa0980450 MtrunA17_Chr6g0460911 83.750 560 62 2 172 702 60 619 0.0 934
Msa0980450 MtrunA17_Chr6g0460911 63.462 52 15 2 2 52 10 58 2.45e-11 67.0
Msa0980450 MtrunA17_Chr6g0460901 79.102 579 69 4 172 705 58 629 0.0 887
Msa0980450 MtrunA17_Chr6g0460901 68.519 54 11 3 1 52 7 56 1.06e-11 68.2
Msa0980450 MtrunA17_Chr7g0254061 65.563 604 165 2 137 697 2 605 0.0 772
Msa0980450 MtrunA17_Chr7g0254011 57.666 574 197 3 172 699 52 625 0.0 665
Msa0980450 MtrunA17_Chr7g0215781 32.897 535 318 10 158 686 50 549 1.04e-71 243
Msa0980450 MtrunA17_Chr1g0210461 31.351 555 337 10 155 699 45 565 2.30e-68 234
Msa0980450 MtrunA17_Chr1g0160231 36.898 374 217 4 172 539 81 441 3.38e-67 232
Msa0980450 MtrunA17_Chr8g0381021 35.772 369 228 4 172 539 86 446 1.84e-66 230
Msa0980450 MtrunA17_Chr8g0381051 31.380 529 321 9 172 697 91 580 2.27e-63 221
Msa0980450 MtrunA17_Chr8g0381031 35.772 369 228 4 172 539 87 447 8.14e-63 220
Msa0980450 MtrunA17_Chr2g0296921 34.783 368 231 4 173 539 87 446 3.47e-62 218
Msa0980450 MtrunA17_Chr8g0358951 29.077 509 326 7 173 681 94 567 2.77e-58 207
Msa0980450 MtrunA17_Chr4g0055481 29.051 506 326 8 185 688 70 544 9.61e-53 191
Msa0980450 MtrunA17_Chr6g0463361 26.531 245 148 6 439 681 1 215 7.26e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0980450 AT1G58030.1 58.795 614 205 4 132 697 9 622 0.0 707
Msa0980450 AT5G36940.1 61.552 554 185 3 172 697 43 596 0.0 686
Msa0980450 AT3G03720.1 60.109 549 192 2 172 693 38 586 0.0 678
Msa0980450 AT1G05940.1 33.582 536 314 10 159 685 50 552 7.95e-75 252
Msa0980450 AT4G21120.1 32.580 531 314 10 172 696 84 576 2.12e-72 246
Msa0980450 AT1G05940.3 34.387 506 290 10 159 655 50 522 6.11e-71 241
Msa0980450 AT1G05940.4 35.123 447 259 8 159 595 50 475 8.84e-68 231
Msa0980450 AT3G10600.1 29.567 531 305 10 178 686 76 559 1.10e-63 222
Msa0980450 AT2G34960.1 29.699 532 318 11 173 694 79 564 1.47e-63 222
Msa0980450 AT5G04770.1 30.297 538 337 9 157 686 54 561 2.28e-63 221
Msa0980450 AT1G17120.1 29.354 511 326 8 171 681 94 569 1.72e-57 206
Msa0980450 AT1G05940.2 30.388 464 284 7 230 685 2 434 3.10e-53 190
Msa0980450 AT5G04770.2 34.795 342 217 4 172 508 71 411 3.11e-53 189
Msa0980450 AT4G21120.2 30.702 456 274 9 246 696 8 426 4.34e-49 179
Msa0980450 AT3G10600.2 27.174 460 266 10 249 686 1 413 6.09e-45 167
Msa0980450 AT5G04770.3 28.153 444 283 8 249 686 1 414 1.58e-41 157

Find 194 sgRNAs with CRISPR-Local

Find 2 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTTACCACTTTCATTTA+TGG 0.116406 6_4:-18613431 Msa0980450:CDS
ATAACGAAATGTGACTATTT+TGG 0.162632 6_4:-18615444 Msa0980450:five_prime_UTR
CATAGTAGCTGGTGGTTATT+TGG 0.174089 6_4:-18611622 Msa0980450:CDS
CGTTATTTCATAGCGATTAA+AGG 0.204717 6_4:+18615461 None:intergenic
TGATTATAGGCTGCACTTAT+TGG 0.205917 6_4:-18612699 Msa0980450:intron
GAGTTTGTTGGTAGTAATTA+TGG 0.208376 6_4:-18615391 Msa0980450:CDS
AAATATTATTTATGGTTTAT+AGG 0.222718 6_4:-18607115 Msa0980450:intron
GGTGGTGTGGCGTTGTTGTC+TGG 0.224427 6_4:-18614622 Msa0980450:CDS
CCGATTACCCTTACCCTTTA+GGG 0.230971 6_4:+18611568 None:intergenic
AACAAATCAAATTGAAAATA+TGG 0.236775 6_4:-18615413 Msa0980450:exon
TTAAAAGCACTATACCAAAT+TGG 0.252668 6_4:+18612894 None:intergenic
TTATACAAGCCTATCTCATT+AGG 0.264676 6_4:-18607235 Msa0980450:three_prime_UTR
GCCATAGCCATCAAAATTCT+TGG 0.274318 6_4:+18610234 None:intergenic
GTTATTATTTATGTATTCTA+TGG 0.277624 6_4:-18607334 Msa0980450:CDS
TAAAAGCACTATACCAAATT+GGG 0.289930 6_4:+18612895 None:intergenic
GCCGATTACCCTTACCCTTT+AGG 0.295570 6_4:+18611567 None:intergenic
GGAGAGTGGGGAAGCGGCGG+AGG 0.303918 6_4:-18614573 Msa0980450:CDS
TGCAGGGTTGCTTGGTTGAT+TGG 0.308815 6_4:-18612976 Msa0980450:intron
AAGCCTTAAAATAACTAATT+CGG 0.313014 6_4:-18615507 None:intergenic
ATAGTATCTATTGTTGTTGT+TGG 0.319564 6_4:-18610814 Msa0980450:CDS
GGGTTGGCATGGTTCGGTTC+GGG 0.320208 6_4:-18615079 Msa0980450:CDS
TGGCCTTGTTCCTGCAGCCT+TGG 0.321304 6_4:-18610133 Msa0980450:CDS
TCTGAGGGTGGTGGTGGTGG+TGG 0.322291 6_4:-18614640 Msa0980450:CDS
GGGGTTGGCATGGTTCGGTT+CGG 0.324382 6_4:-18615080 Msa0980450:CDS
ATGGATGTCTCTCAACTTGC+TGG 0.324460 6_4:-18610102 Msa0980450:CDS
AACATACTCGTCTGCACTAT+TGG 0.326499 6_4:+18614736 None:intergenic
CGTTGTTGTCTGGGATTGTT+AGG 0.327631 6_4:-18614612 Msa0980450:CDS
TTGATAAGAATGCAAGTTAT+AGG 0.334024 6_4:+18608005 None:intergenic
GGAGGTTGAGGCGCGGAGAG+TGG 0.334268 6_4:-18614587 Msa0980450:CDS
GGATCTGAGGGTGGTGGTGG+TGG 0.345908 6_4:-18614643 Msa0980450:CDS
AAGAAGGTTGATTCTAATGT+TGG 0.348906 6_4:-18615328 Msa0980450:CDS
ATAGTAGCTGGTGGTTATTT+GGG 0.353899 6_4:-18611621 Msa0980450:CDS
AAGGAGAGGGTTGTTGATGC+TGG 0.358474 6_4:-18614547 Msa0980450:CDS
TCAGTTAGATTTACATTATG+TGG 0.359468 6_4:-18608318 Msa0980450:CDS
GACAGCAAAAGCAGAAGATA+TGG 0.360810 6_4:+18610756 None:intergenic
CCACTTTCATTTATGGTAGC+TGG 0.361159 6_4:-18613424 Msa0980450:CDS
CTTGGTTGCGAGTTTCTATA+TGG 0.363411 6_4:-18607369 Msa0980450:CDS
GATTCTAAGTCAATGGAGTA+TGG 0.365680 6_4:-18609040 Msa0980450:intron
GCAGAAGATATGGGAGTGTT+AGG 0.368415 6_4:+18610766 None:intergenic
GAGTGGGGAAGCGGCGGAGG+GGG 0.369059 6_4:-18614570 Msa0980450:CDS
ACCATTCCATTTACCCCAAA+TGG 0.370452 6_4:+18611453 None:intergenic
CCTCAAAGAGATTTGCCAAT+TGG 0.371631 6_4:-18610877 Msa0980450:CDS
AAAACTGTTACGGTTACTAT+TGG 0.372679 6_4:-18615053 Msa0980450:intron
GCGTCAACTTCGATTGTGTT+TGG 0.372870 6_4:-18614664 Msa0980450:CDS
TTTCCAAGGGAAGGGGACTA+TGG 0.374719 6_4:-18615108 Msa0980450:CDS
GTGACTATGGTACTCTTAAC+AGG 0.375841 6_4:+18610156 None:intergenic
AGGGCAAACATCACACCAAT+TGG 0.378606 6_4:-18607215 Msa0980450:three_prime_UTR
CCAATTGGCAAATCTCTTTG+AGG 0.382184 6_4:+18610877 None:intergenic
GGATTCAAAACTGGATGGAT+TGG 0.383019 6_4:-18611600 Msa0980450:CDS
TTATCATATCAACATATTCC+TGG 0.387608 6_4:-18612651 Msa0980450:CDS
GGTTATTTGGGATTCAAAAC+TGG 0.389451 6_4:-18611609 Msa0980450:CDS
AATTGGTTCTTGTCGAGAAT+GGG 0.391750 6_4:+18609063 None:intergenic
CATCTTGATCTATACATGTT+AGG 0.395167 6_4:+18608259 None:intergenic
GTAATAGCATTCATATGTTT+AGG 0.396182 6_4:-18608496 Msa0980450:CDS
TACAGATACATTATAAATGC+TGG 0.401124 6_4:-18610537 Msa0980450:intron
CTTCTTGTACATATCTTCTC+AGG 0.402576 6_4:-18608450 Msa0980450:intron
GGGTTGCTTGGTTGATTGGC+TGG 0.404887 6_4:-18612972 Msa0980450:CDS
ACTTAGGGGTTGGCATGGTT+CGG 0.405249 6_4:-18615085 Msa0980450:CDS
TCCATTGACTTAGAATCAAT+TGG 0.405897 6_4:+18609046 None:intergenic
AGCTCTTTGTTATGCTGAGT+TGG 0.406105 6_4:-18613383 Msa0980450:CDS
TTTGGATCTGAGGGTGGTGG+TGG 0.407237 6_4:-18614646 Msa0980450:CDS
CAATTGGTTCTTGTCGAGAA+TGG 0.410537 6_4:+18609062 None:intergenic
GTGGTGTGGCGTTGTTGTCT+GGG 0.414478 6_4:-18614621 Msa0980450:CDS
GGAGGTATGGACAACTTGCC+AGG 0.415029 6_4:-18612678 Msa0980450:CDS
AGGAACAAGGCCAGTGACTA+TGG 0.416861 6_4:+18610143 None:intergenic
TGTCTCTCAACTTGCTGGCT+TGG 0.419511 6_4:-18610097 Msa0980450:intron
ATTGAAAATATGGAGTTTGT+TGG 0.422081 6_4:-18615403 Msa0980450:exon
TATACAAGCCTATCTCATTA+GGG 0.427979 6_4:-18607234 Msa0980450:three_prime_UTR
ACCAATTGATTCTAAGTCAA+TGG 0.429801 6_4:-18609047 Msa0980450:CDS
CTATACCTCTTCAGCAGTGC+TGG 0.432409 6_4:+18611380 None:intergenic
GATTGTTAGGTGGGAGGTTG+AGG 0.432674 6_4:-18614599 Msa0980450:CDS
CATTATGCTTACATATGTCT+TGG 0.443633 6_4:-18613328 Msa0980450:CDS
TTGTGGTATTCATTATTACT+TGG 0.444965 6_4:-18612596 Msa0980450:CDS
AGAGTGGGGAAGCGGCGGAG+GGG 0.447726 6_4:-18614571 Msa0980450:CDS
AAGAAGGTTCATCATGTTCA+TGG 0.455288 6_4:-18615366 Msa0980450:CDS
TTAACTCTGGAAGTTGTGAT+TGG 0.456165 6_4:-18612946 Msa0980450:CDS
GGTAGTAATTATGGAGAAGA+AGG 0.458569 6_4:-18615382 Msa0980450:CDS
TACAAGAAGCTGCAAATGTA+AGG 0.458918 6_4:+18608464 None:intergenic
TGGGGCAATGGAACTTCAAT+TGG 0.458969 6_4:+18609082 None:intergenic
AATCACAACTTCCAGAGTTA+AGG 0.461927 6_4:+18612948 None:intergenic
TTACCAATTGCCATGAGATC+AGG 0.463145 6_4:+18615256 None:intergenic
TTCAGGTTTCAAATGTCCAT+TGG 0.465319 6_4:-18608039 Msa0980450:intron
GTGTTTGGATCTGAGGGTGG+TGG 0.465564 6_4:-18614649 Msa0980450:CDS
AGGCGCGGAGAGTGGGGAAG+CGG 0.465713 6_4:-18614579 Msa0980450:CDS
CTCTTTGGTTTCCAAGGGAA+GGG 0.467553 6_4:-18615116 Msa0980450:CDS
ACTATGTTTGCAGGGTTGCT+TGG 0.468317 6_4:-18612984 Msa0980450:intron
GATTGGCTGGTCCTTAACTC+TGG 0.468912 6_4:-18612959 Msa0980450:CDS
TGTTAACTCTTTGGTTTCCA+AGG 0.469195 6_4:-18615122 Msa0980450:CDS
TATCATATCAACATATTCCT+GGG 0.469478 6_4:-18612650 Msa0980450:CDS
TTGATGTTTGTCATAGTAGC+TGG 0.471874 6_4:-18611633 Msa0980450:CDS
AGATTTACATTATGTGGAGT+TGG 0.473135 6_4:-18608312 Msa0980450:CDS
GTTCCTGATCTCATGGCAAT+TGG 0.474248 6_4:-18615259 Msa0980450:intron
ATCGAAAATGCCAAGGCTGC+AGG 0.474824 6_4:+18610123 None:intergenic
GCTTCATTGTCTCTGTGTTG+TGG 0.475348 6_4:-18610847 Msa0980450:CDS
CCTCTTTGATCCCGGTACAT+AGG 0.475722 6_4:+18612571 None:intergenic
AGAAGGTTGATTCTAATGTT+GGG 0.477162 6_4:-18615327 Msa0980450:CDS
TTGATGCAGATAATGGTAAG+AGG 0.477601 6_4:-18608537 Msa0980450:intron
TTCGATTGTGTTTGGATCTG+AGG 0.478693 6_4:-18614656 Msa0980450:CDS
GTCTGGGATTGTTAGGTGGG+AGG 0.479086 6_4:-18614605 Msa0980450:CDS
GGATACAAGTTAGACCCAAT+TGG 0.480952 6_4:+18607200 None:intergenic
GAGGTTGAGGCGCGGAGAGT+GGG 0.483864 6_4:-18614586 Msa0980450:CDS
GGCTGCACTTATTGGAGGTA+TGG 0.486263 6_4:-18612691 Msa0980450:CDS
TTGGATATGAACTCCCTAAA+GGG 0.487322 6_4:-18611581 Msa0980450:intron
GTTGTCTGGGATTGTTAGGT+GGG 0.487881 6_4:-18614608 Msa0980450:CDS
TGCTGTCAATGGAATGCAAT+GGG 0.489792 6_4:-18610740 Msa0980450:CDS
TTATAGGCTGCACTTATTGG+AGG 0.492314 6_4:-18612696 Msa0980450:intron
AATGAAGCTGCAATTCCAAT+TGG 0.492493 6_4:+18610862 None:intergenic
GGAAGGGGACTATGGACTTA+GGG 0.496272 6_4:-18615100 Msa0980450:CDS
ATAAATGAAAGTGGTAAAGA+TGG 0.496794 6_4:+18613433 None:intergenic
TTTGAGCTCTGTTTCAGTGA+CGG 0.496981 6_4:-18614686 Msa0980450:CDS
TCCATTTGGGGTAAATGGAA+TGG 0.497630 6_4:-18611454 Msa0980450:CDS
ACAGATACATTATAAATGCT+GGG 0.498551 6_4:-18610536 Msa0980450:intron
CGCGGAGAGTGGGGAAGCGG+CGG 0.499102 6_4:-18614576 Msa0980450:CDS
GATGATGCAAGACACAACTT+TGG 0.501549 6_4:-18608240 Msa0980450:CDS
TTGTTCGAAGCCGCATGTGG+AGG 0.501935 6_4:-18614761 Msa0980450:CDS
GTTCGGGATGAAAACTGTTA+CGG 0.502566 6_4:-18615063 Msa0980450:CDS
TATGGACTTAGGGGTTGGCA+TGG 0.503928 6_4:-18615090 Msa0980450:CDS
CGTATTTACTTATCAGTCGT+GGG 0.504378 6_4:-18607980 Msa0980450:intron
AGTCCATAGTCCCCTTCCCT+TGG 0.504416 6_4:+18615105 None:intergenic
GGGAAGGGGACTATGGACTT+AGG 0.509382 6_4:-18615101 Msa0980450:CDS
TCGTATTTACTTATCAGTCG+TGG 0.510083 6_4:-18607981 Msa0980450:CDS
GAGAGTGGGGAAGCGGCGGA+GGG 0.510125 6_4:-18614572 Msa0980450:CDS
TTTACATTATGTGGAGTTGG+TGG 0.511270 6_4:-18608309 Msa0980450:CDS
ACTCTGGAAGTTGTGATTGG+TGG 0.513114 6_4:-18612943 Msa0980450:CDS
GCACTATGCTCATCATTGCT+TGG 0.517145 6_4:-18610504 Msa0980450:CDS
AATTGCCAGCACTGCTGAAG+AGG 0.520469 6_4:-18611385 Msa0980450:intron
GGGCAAACATCACACCAATT+GGG 0.525831 6_4:-18607214 Msa0980450:three_prime_UTR
GAAACAGAGCTCAAAGAAAA+CGG 0.525898 6_4:+18614694 None:intergenic
GAAGGGGACTATGGACTTAG+GGG 0.530710 6_4:-18615099 Msa0980450:CDS
ATTACTTGGATCCTATGTAC+CGG 0.532532 6_4:-18612582 Msa0980450:CDS
ACTCTTTGGTTTCCAAGGGA+AGG 0.532932 6_4:-18615117 Msa0980450:CDS
AAAAGTTACCTCTTTGATCC+CGG 0.533394 6_4:+18612563 None:intergenic
TTCTAGTTGGAACTGAAGCT+AGG 0.534193 6_4:-18613468 Msa0980450:CDS
CCAGCTACCATAAATGAAAG+TGG 0.535430 6_4:+18613424 None:intergenic
TGTTCGAAGCCGCATGTGGA+GGG 0.535811 6_4:-18614760 Msa0980450:CDS
AGACACAACTTTGGAAACAC+TGG 0.537540 6_4:-18608231 Msa0980450:CDS
ATTGGATATGAACTCCCTAA+AGG 0.539555 6_4:-18611582 Msa0980450:CDS
GTTAACTCTTTGGTTTCCAA+GGG 0.543993 6_4:-18615121 Msa0980450:CDS
GGCAATGGAACTTCAATTGG+TGG 0.545806 6_4:+18609085 None:intergenic
GCTTGTATAATTAAGCAAGA+AGG 0.547415 6_4:+18607248 None:intergenic
TCTTTGGTTTCCAAGGGAAG+GGG 0.549646 6_4:-18615115 Msa0980450:CDS
TGTTGTCTGGGATTGTTAGG+TGG 0.549962 6_4:-18614609 Msa0980450:CDS
TTAGGATTGATATCATAGTA+AGG 0.551985 6_4:+18610784 None:intergenic
AAAAGCACTATACCAAATTG+GGG 0.552495 6_4:+18612896 None:intergenic
ATGCCAAGGCTGCAGGAACA+AGG 0.552498 6_4:+18610130 None:intergenic
CTTACATATGTCTTGGTGAA+GGG 0.552617 6_4:-18613321 Msa0980450:intron
AGACCCACTTGCAGCAATTG+TGG 0.553506 6_4:-18612613 Msa0980450:CDS
GCTTGGAACAATGTTGCCCC+AGG 0.553937 6_4:-18610487 Msa0980450:intron
TCAGGTGTTGGTGCAACAAT+CGG 0.555805 6_4:-18613505 Msa0980450:intron
ACAGCAAAAGCAGAAGATAT+GGG 0.556454 6_4:+18610757 None:intergenic
ATTTGGGATTCAAAACTGGA+TGG 0.559171 6_4:-18611605 Msa0980450:CDS
TGTCTTCTACAGGTTAGTGT+AGG 0.561280 6_4:-18609163 Msa0980450:intron
GATGTTTGCCCTAATGAGAT+AGG 0.563066 6_4:+18607226 None:intergenic
TCGATTGTGTTTGGATCTGA+GGG 0.564257 6_4:-18614655 Msa0980450:CDS
GTTAACAGTTCCTGATCTCA+TGG 0.565046 6_4:-18615266 Msa0980450:CDS
GCTTACATATGTCTTGGTGA+AGG 0.566978 6_4:-18613322 Msa0980450:CDS
TGATAAGCACTTCCAGCAGA+AGG 0.569318 6_4:+18613349 None:intergenic
TCTAGTTGGAACTGAAGCTA+GGG 0.575911 6_4:-18613467 Msa0980450:CDS
TATATATACGACTATTGAAG+TGG 0.577246 6_4:+18607158 None:intergenic
AGGTTGAGGCGCGGAGAGTG+GGG 0.581904 6_4:-18614585 Msa0980450:CDS
ATGCAAGTTATAGGCAGCAG+AGG 0.583004 6_4:+18608014 None:intergenic
GTTGGTGCAACAATCGGTGC+TGG 0.583920 6_4:-18613499 Msa0980450:CDS
CCGTTGTTCGAAGCCGCATG+TGG 0.588327 6_4:-18614764 Msa0980450:CDS
GCTGGTGTATATGTTCTAGT+TGG 0.588465 6_4:-18613481 Msa0980450:CDS
AAAAGATTCAGTTCATGTGA+AGG 0.589890 6_4:-18607293 Msa0980450:CDS
GGGACTATGGACTTAGGGGT+TGG 0.590741 6_4:-18615095 Msa0980450:CDS
TTGATGGCTATGGCAAGGGA+TGG 0.591651 6_4:-18610225 Msa0980450:CDS
TTACTTGGATCCTATGTACC+GGG 0.595365 6_4:-18612581 Msa0980450:CDS
GTTAAGAGTACCATAGTCAC+TGG 0.596497 6_4:-18610153 Msa0980450:CDS
CCACATGCGGCTTCGAACAA+CGG 0.597185 6_4:+18614764 None:intergenic
GGTGGAGCTGTTGTTGCTCG+TGG 0.597637 6_4:-18612925 Msa0980450:CDS
ACATGGAGTGCAACTTGCGA+AGG 0.598185 6_4:-18615290 Msa0980450:CDS
AATACCACAATTGCTGCAAG+TGG 0.598983 6_4:+18612609 None:intergenic
ATAGGCAGCAGAGGCACCAA+TGG 0.600410 6_4:+18608023 None:intergenic
GTATGTTTCTTCTTGTGCTG+CGG 0.602370 6_4:-18614720 Msa0980450:CDS
TCTACAACAATATCAATCCC+AGG 0.604451 6_4:+18612633 None:intergenic
ATTGGTTCTTGTCGAGAATG+GGG 0.604618 6_4:+18609064 None:intergenic
TCTTGTCGAGAATGGGGCAA+TGG 0.605377 6_4:+18609070 None:intergenic
GTTTCTATATGGCTAGCAGT+AGG 0.612922 6_4:-18607358 Msa0980450:CDS
ATGTTTGTCATAGTAGCTGG+TGG 0.619847 6_4:-18611630 Msa0980450:CDS
GACATCCATCGAAAATGCCA+AGG 0.620846 6_4:+18610116 None:intergenic
TTGCTGTCAATGGAATGCAA+TGG 0.642365 6_4:-18610741 Msa0980450:CDS
AAAGCACTATACCAAATTGG+GGG 0.644443 6_4:+18612897 None:intergenic
TAATTAAGCAAGAAGGCTTG+TGG 0.645384 6_4:+18607255 None:intergenic
CAGATACATTATAAATGCTG+GGG 0.645816 6_4:-18610535 Msa0980450:CDS
ACTGAAGCTAGGGAACATGC+TGG 0.645900 6_4:-18613457 Msa0980450:CDS
GGGAATAACAAGAATTCACA+TGG 0.650087 6_4:-18615307 Msa0980450:CDS
TGGTACTCTTAACAGGAACA+TGG 0.657797 6_4:+18610163 None:intergenic
CACAACTTTGGAAACACTGG+AGG 0.659750 6_4:-18608228 Msa0980450:intron
ATTGTGTTTGGATCTGAGGG+TGG 0.660245 6_4:-18614652 Msa0980450:CDS
ACTATTGGTCCCTCCACATG+CGG 0.675254 6_4:+18614751 None:intergenic
ATACCACAATTGCTGCAAGT+GGG 0.677479 6_4:+18612610 None:intergenic
TTAGGTGGGAGGTTGAGGCG+CGG 0.701551 6_4:-18614594 Msa0980450:CDS
CCTATGTACCGGGATCAAAG+AGG 0.735153 6_4:-18612571 Msa0980450:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACGAAATGTGACTATTT+TGG - chr6_4:18607013-18607032 Msa0980450:three_prime_UTR 25.0%
! CGTTATTTCATAGCGATTAA+AGG + chr6_4:18606999-18607018 None:intergenic 30.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 18606957 18615522 18606957 ID=Msa0980450;Name=Msa0980450
chr6_4 mRNA 18606957 18615522 18606957 ID=Msa0980450-mRNA-1;Parent=Msa0980450;Name=Msa0980450-mRNA-1;_AED=0.20;_eAED=0.20;_QI=106|0.68|0.70|0.94|0.93|0.88|17|263|705
chr6_4 exon 18606957 18607124 18606957 ID=Msa0980450-mRNA-1:exon:5822;Parent=Msa0980450-mRNA-1
chr6_4 exon 18607163 18607399 18607163 ID=Msa0980450-mRNA-1:exon:5821;Parent=Msa0980450-mRNA-1
chr6_4 exon 18607981 18608056 18607981 ID=Msa0980450-mRNA-1:exon:5820;Parent=Msa0980450-mRNA-1
chr6_4 exon 18608229 18608341 18608229 ID=Msa0980450-mRNA-1:exon:5819;Parent=Msa0980450-mRNA-1
chr6_4 exon 18608451 18608550 18608451 ID=Msa0980450-mRNA-1:exon:5818;Parent=Msa0980450-mRNA-1
chr6_4 exon 18609041 18609173 18609041 ID=Msa0980450-mRNA-1:exon:5817;Parent=Msa0980450-mRNA-1
chr6_4 exon 18610098 18610256 18610098 ID=Msa0980450-mRNA-1:exon:5816;Parent=Msa0980450-mRNA-1
chr6_4 exon 18610488 18610554 18610488 ID=Msa0980450-mRNA-1:exon:5815;Parent=Msa0980450-mRNA-1
chr6_4 exon 18610736 18610908 18610736 ID=Msa0980450-mRNA-1:exon:5814;Parent=Msa0980450-mRNA-1
chr6_4 exon 18611386 18611482 18611386 ID=Msa0980450-mRNA-1:exon:5813;Parent=Msa0980450-mRNA-1
chr6_4 exon 18611582 18611700 18611582 ID=Msa0980450-mRNA-1:exon:5812;Parent=Msa0980450-mRNA-1
chr6_4 exon 18612572 18612712 18612572 ID=Msa0980450-mRNA-1:exon:5811;Parent=Msa0980450-mRNA-1
chr6_4 exon 18612909 18612993 18612909 ID=Msa0980450-mRNA-1:exon:5810;Parent=Msa0980450-mRNA-1
chr6_4 exon 18613322 18613523 18613322 ID=Msa0980450-mRNA-1:exon:5809;Parent=Msa0980450-mRNA-1
chr6_4 exon 18614545 18614807 18614545 ID=Msa0980450-mRNA-1:exon:5808;Parent=Msa0980450-mRNA-1
chr6_4 exon 18615054 18615144 18615054 ID=Msa0980450-mRNA-1:exon:5807;Parent=Msa0980450-mRNA-1
chr6_4 exon 18615260 18615522 18615260 ID=Msa0980450-mRNA-1:exon:5806;Parent=Msa0980450-mRNA-1
chr6_4 five_prime_UTR 18615417 18615522 18615417 ID=Msa0980450-mRNA-1:five_prime_utr;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18615260 18615416 18615260 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18615054 18615144 18615054 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18614545 18614807 18614545 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18613322 18613523 18613322 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18612909 18612993 18612909 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18612572 18612712 18612572 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18611582 18611700 18611582 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18611386 18611482 18611386 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18610736 18610908 18610736 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18610488 18610554 18610488 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18610098 18610256 18610098 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18609041 18609173 18609041 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18608451 18608550 18608451 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18608229 18608341 18608229 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18607981 18608056 18607981 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 CDS 18607258 18607399 18607258 ID=Msa0980450-mRNA-1:cds;Parent=Msa0980450-mRNA-1
chr6_4 three_prime_UTR 18607163 18607257 18607163 ID=Msa0980450-mRNA-1:three_prime_utr;Parent=Msa0980450-mRNA-1
chr6_4 three_prime_UTR 18606957 18607124 18606957 ID=Msa0980450-mRNA-1:three_prime_utr;Parent=Msa0980450-mRNA-1
Gene Sequence

>Msa0980450

ATGGAGTTTGTTGGTAGTAATTATGGAGAAGAAGGTTCATCATGTTCATGGAGTTTTTTAAGAAGGAAGAAGGTTGATTCTAATGTTGGGAATAACAAGAATTCACATGGAGTGCAACTTGCGAAGGAGTTAACAGTTCCTGATCTCATGGCAATTGTGTTAACTCTTTGGTTTCCAAGGGAAGGGGACTATGGACTTAGGGGTTGGCATGGTTCGGTTCGGGATGAAAACTGTTACGGTTACTATTGTGTAAAAATATCAATATGGAGCCGTTGTTCGAAGCCGCATGTGGAGGGACCAATAGTGCAGACGAGTATGTTTCTTCTTGTGCTGCGGAAACCGTTTTCTTTGAGCTCTGTTTCAGTGACGGCGTCAACTTCGATTGTGTTTGGATCTGAGGGTGGTGGTGGTGGTGGTGTGGCGTTGTTGTCTGGGATTGTTAGGTGGGAGGTTGAGGCGCGGAGAGTGGGGAAGCGGCGGAGGGGGAAGGAGAGGGTTGTTGATGCTGGAAGTGTTGGTGCAACAATCGGTGCTGGTGTATATGTTCTAGTTGGAACTGAAGCTAGGGAACATGCTGGACCATCTTTACCACTTTCATTTATGGTAGCTGGAATAGCAGCAGCTCTTTCAGCTCTTTGTTATGCTGAGTTGGCTAGTCGTTTTCCTTCTGCTGGAAGTGCTTATCATTATGCTTACATATGTCTTGGTGAAGGGGTTGCTTGGTTGATTGGCTGGTCCTTAACTCTGGAAGTTGTGATTGGTGGAGCTGTTGTTGCTCGTGGCATAACCCCCAATTTGGCTGCACTTATTGGAGGTATGGACAACTTGCCAGGATTTTTATCATATCAACATATTCCTGGGATTGATATTGTTGTAGACCCACTTGCAGCAATTGTGGTATTCATTATTACTTGGATCCTATGTACCGGGATCAAAGAGAGTGCAGTGATACAAAGTATAGTCACATCAATGAATATATGTGCTTTGATGTTTGTCATAGTAGCTGGTGGTTATTTGGGATTCAAAACTGGATGGATTGGATATGAACTCCCTAAAGGGTATTTTCCATTTGGGGTAAATGGAATGGTTGCTAGTTCTGCAACAGCCTTTTTTGCATACTTAGGTTTTGAAGCAATTGCCAGCACTGCTGAAGAGGTGAAAAATCCTCAAAGAGATTTGCCAATTGGAATTGCAGCTTCATTGTCTCTGTGTTGTGGATTGTATATGATAGTATCTATTGTTGTTGTTGGTTTAGTACCTTACTATGATATCAATCCTAACACTCCCATATCTTCTGCTTTTGCTGTCAATGGAATGCAATGGGCAGCATACATTATAAATGCTGGGGCTCTTACAGCACTATGCTCATCATTGCTTGGAACAATGTTGCCCCAGCCAAGAATTTTGATGGCTATGGCAAGGGATGGACTATTGCCACCATTTTTTTCTGACATAAATAAGCACAGCCATGTTCCTGTTAAGAGTACCATAGTCACTGGCCTTGTTCCTGCAGCCTTGGCATTTTCGATGGATGTCTCTCAACTTGCTGGCTTGGTTAGTGTAGGCACTCTTATTGCATTCACAATATCAGCAATAACTGTGTTGATAGTCAGATATATACCACCAATTGAAGTTCCATTGCCCCATTCTCGACAAGAACCAATTGATTCTAAGTCAATGGAGTATGATAATGGTAAGAGGAAAAAAATTGTTGGCTGTGTAATAGCATTCATATGTTTAGGTGTATTTGTCCTTACATTTGCAGCTTCTTGTACATATCTTCTCAGTTCAGTTAGATTTACATTATGTGGAGTTGGTGGCATTGTTCTTTTGTCTGGCTTTGTCTTCCTAACATGTATAGATCAAGATGATGCAAGACACAACTTTGGAAACACTGGAGGTTTCAAATGTCCATTGGTGCCTCTGCTGCCTATAACTTGCATTCTTATCAATTCGTATTTACTTATCAGTCGTGGGGGTGCTACTTGGTTGCGAGTTTCTATATGGCTAGCAGTAGGAGTTATTATTTATGTATTCTATGGTCGAACCCATAGCTCTCTAAAAGATTCAGTTCATGTGAAGGCTGCTCAAGATAATACCACAAGCCTTCTTGCTTAA
Protein sequence

>Msa0980450

MEFVGSNYGEEGSSCSWSFLRRKKVDSNVGNNKNSHGVQLAKELTVPDLMAIVLTLWFPREGDYGLRGWHGSVRDENCYGYYCVKISIWSRCSKPHVEGPIVQTSMFLLVLRKPFSLSSVSVTASTSIVFGSEGGGGGGVALLSGIVRWEVEARRVGKRRRGKERVVDAGSVGATIGAGVYVLVGTEAREHAGPSLPLSFMVAGIAAALSALCYAELASRFPSAGSAYHYAYICLGEGVAWLIGWSLTLEVVIGGAVVARGITPNLAALIGGMDNLPGFLSYQHIPGIDIVVDPLAAIVVFIITWILCTGIKESAVIQSIVTSMNICALMFVIVAGGYLGFKTGWIGYELPKGYFPFGVNGMVASSATAFFAYLGFEAIASTAEEVKNPQRDLPIGIAASLSLCCGLYMIVSIVVVGLVPYYDINPNTPISSAFAVNGMQWAAYIINAGALTALCSSLLGTMLPQPRILMAMARDGLLPPFFSDINKHSHVPVKSTIVTGLVPAALAFSMDVSQLAGLVSVGTLIAFTISAITVLIVRYIPPIEVPLPHSRQEPIDSKSMEYDNGKRKKIVGCVIAFICLGVFVLTFAASCTYLLSSVRFTLCGVGGIVLLSGFVFLTCIDQDDARHNFGNTGGFKCPLVPLLPITCILINSYLLISRGGATWLRVSIWLAVGVIIYVFYGRTHSSLKDSVHVKAAQDNTTSLLA*