AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999490 MtrunA17_Chr6g0485681 99.076 433 4 0 3 435 47 479 0.0 884
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999490 AT3G24530.2 79.215 433 90 0 3 435 1 433 0.0 727
Msa0999490 AT3G24530.1 79.215 433 90 0 3 435 49 481 0.0 726
Msa0999490 AT3G24530.3 79.949 389 78 0 47 435 1 389 0.0 657

Find 119 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTAATGCAAGAGAAAATT+TGG 0.194417 6_4:-81539912 Msa0999490:CDS
GCAACCATTCTTTGGCAATT+AGG 0.236259 6_4:-81539798 Msa0999490:exon
TCTTTGGCAATTAGGTGTTT+TGG 0.238936 6_4:-81539790 Msa0999490:exon
ACAATAGGTGAATCCTTCTT+TGG 0.267190 6_4:-81539494 Msa0999490:three_prime_UTR
CAGCGAAACCAAAGCTAAAA+TGG 0.303855 6_4:-81541180 Msa0999490:CDS
CCTTTGTATCAACGCTTCTA+TGG 0.314985 6_4:+81539991 None:intergenic
GCTGGTCGATCTCAAATTAA+TGG 0.316334 6_4:-81542304 Msa0999490:five_prime_UTR
ATGTTTCTGCAGGAAACAAT+AGG 0.317133 6_4:-81542163 Msa0999490:CDS
TTAGCACAGCAACCATTCTT+TGG 0.323710 6_4:-81539806 Msa0999490:CDS
GCAGAAGGTAGTTTGCTCTA+TGG 0.334627 6_4:-81540046 Msa0999490:CDS
TCCCTAAGTTTATCATTTCC+AGG 0.339640 6_4:+81541257 None:intergenic
CACCTTTACACCTTGCAGTT+TGG 0.339947 6_4:-81541479 Msa0999490:CDS
TGAGATAGAGGCTAAGAATA+TGG 0.343681 6_4:-81542093 Msa0999490:intron
CACAAAACCTTTCCCAAAGA+AGG 0.356016 6_4:+81539481 None:intergenic
AAGGAACTTTCAAATATTGT+AGG 0.359061 6_4:-81541146 Msa0999490:CDS
GACAAACTCGCCAACTAAAT+CGG 0.369343 6_4:+81540902 None:intergenic
TTATCATCTGATTTCTGCAT+TGG 0.376034 6_4:+81540277 None:intergenic
GAAGCTATTACTCGTTTCAT+TGG 0.382036 6_4:+81540169 None:intergenic
ATAATTAATTAAAATACTTA+AGG 0.385823 6_4:+81539324 None:intergenic
TCAAAATCCACATTGAAATC+TGG 0.391184 6_4:+81539692 None:intergenic
ATGGCCTTTCTTGGGAATCC+TGG 0.392337 6_4:-81541017 Msa0999490:CDS
TACAGTCAAAACTAAGTCTT+AGG 0.395011 6_4:+81539888 None:intergenic
TGAAAATACAACTACGAAAG+TGG 0.398331 6_4:-81541112 Msa0999490:CDS
GATTTAGTTGGCGAGTTTGT+CGG 0.410464 6_4:-81540900 Msa0999490:CDS
AAAATTGTAGTAATATTTGC+AGG 0.411416 6_4:-81540202 Msa0999490:CDS
AACTTAGGGAACTATTAAAC+TGG 0.411802 6_4:-81541244 Msa0999490:CDS
TGAATTGAGTTTGCCACAAT+AGG 0.414823 6_4:-81539509 Msa0999490:three_prime_UTR
ATCGGAGAGGGTTGAGATAG+AGG 0.417047 6_4:-81542105 Msa0999490:CDS
TTCTCTTGCATTAATCATCA+TGG 0.422736 6_4:+81539919 None:intergenic
GGACTACGGGTTGGAAGCCT+TGG 0.424972 6_4:-81540254 Msa0999490:CDS
CTCTATGGATTTAAGTTGCA+TGG 0.425673 6_4:-81540031 Msa0999490:CDS
CAATAGGTGAATCCTTCTTT+GGG 0.425740 6_4:-81539493 Msa0999490:three_prime_UTR
AAAATACTGAGCTTAAAATC+TGG 0.426750 6_4:-81539650 Msa0999490:three_prime_UTR
AATCATCTTTCGCAAGGCCC+TGG 0.426961 6_4:-81541275 Msa0999490:CDS
GATGAGAGGCGCAGAGCTCT+TGG 0.439509 6_4:-81541071 Msa0999490:CDS
CCCCTCATATGGCCTTTCTT+GGG 0.440544 6_4:-81541025 Msa0999490:CDS
AATGCAGTTAGCCACATTGA+TGG 0.445641 6_4:-81539738 Msa0999490:three_prime_UTR
GAAGATTTAGAAGCAGGTCT+TGG 0.451968 6_4:-81539833 Msa0999490:CDS
AATGCAGAAATCAGATGATA+AGG 0.455044 6_4:-81540275 Msa0999490:CDS
CTTCATGCTTATCTTACTCT+AGG 0.459635 6_4:-81542258 Msa0999490:five_prime_UTR
TAAAATGGATGAACTTGAAA+AGG 0.462730 6_4:-81541165 Msa0999490:CDS
GTGCCATCTGCATATAAAAC+AGG 0.463011 6_4:+81542198 None:intergenic
TTCCCAAGAAAGGCCATATG+AGG 0.463525 6_4:+81541023 None:intergenic
TAAGGAAACAAATGGCGGTT+TGG 0.465248 6_4:-81539948 Msa0999490:CDS
ACATTGGAAGATTTAGAAGC+AGG 0.466998 6_4:-81539839 Msa0999490:CDS
TCCTGGAACAGGTAAAACTA+TGG 0.467198 6_4:-81541000 Msa0999490:CDS
TTCTAAATCTTCCAATGTAA+TGG 0.467879 6_4:+81539844 None:intergenic
GTATGTTATTCATTCTTATG+AGG 0.468503 6_4:-81539380 Msa0999490:three_prime_UTR
TTCTTTGCTGCCATGTGCAA+TGG 0.474026 6_4:+81541944 None:intergenic
TTTCTTGGGAATCCTGGAAC+AGG 0.477449 6_4:-81541011 Msa0999490:CDS
TGTTTCTGCAGGAAACAATA+GGG 0.478049 6_4:-81542162 Msa0999490:CDS
TTGCACATGGCAGCAAAGAA+TGG 0.480265 6_4:-81541941 Msa0999490:CDS
CATACAAAGAATACAATGTA+AGG 0.481591 6_4:+81539398 None:intergenic
CCCTGGAAATGATAAACTTA+GGG 0.487476 6_4:-81541258 Msa0999490:CDS
AACACCTAATTGCCAAAGAA+TGG 0.492204 6_4:+81539794 None:intergenic
ACACCTCTTCATGTTTCTGC+AGG 0.492960 6_4:-81542173 Msa0999490:CDS
AGAGCTCTTGGTCTGCATGT+TGG 0.493864 6_4:-81541059 Msa0999490:CDS
CTACGGGTTGGAAGCCTTGG+AGG 0.495165 6_4:-81540251 Msa0999490:CDS
GGTGAATCCTTCTTTGGGAA+AGG 0.497368 6_4:-81539488 Msa0999490:three_prime_UTR
ACTATGGTAGCACGCATCCT+TGG 0.502013 6_4:-81540984 Msa0999490:CDS
GCCCTGGAAATGATAAACTT+AGG 0.502459 6_4:-81541259 Msa0999490:CDS
GGCGAGTTTGTCGGTCATAC+CGG 0.502758 6_4:-81540891 Msa0999490:CDS
CCCTAAGTTTATCATTTCCA+GGG 0.515848 6_4:+81541258 None:intergenic
AAATCAGATGATAAGGACTA+CGG 0.525761 6_4:-81540268 Msa0999490:CDS
CGGTCCGAAAACGAGGAAGA+AGG 0.528809 6_4:-81540871 Msa0999490:intron
AGAACTTCTTACCATTACAT+TGG 0.531138 6_4:-81539855 Msa0999490:CDS
AAACAACGTAAGGAAACAAA+TGG 0.531773 6_4:-81539956 Msa0999490:CDS
CCATAGAAGCGTTGATACAA+AGG 0.536377 6_4:-81539991 Msa0999490:CDS
AAAGAAGGATTCACCTATTG+TGG 0.542740 6_4:+81539496 None:intergenic
CCTTTGAATCATCTTTCGCA+AGG 0.544004 6_4:-81541281 Msa0999490:CDS
CCCAAGAAAGGCCATATGAG+GGG 0.545373 6_4:+81541025 None:intergenic
TTAGCCACATTGATGGCCGT+TGG 0.546394 6_4:-81539731 Msa0999490:three_prime_UTR
TCTTCTTGAGTGGCAAGGAT+CGG 0.546943 6_4:-81542123 Msa0999490:CDS
GTACAACGGACCGATTTAGT+TGG 0.547583 6_4:-81540912 Msa0999490:CDS
TAGTTAAATTTCTTCTTGAG+TGG 0.547620 6_4:-81542133 Msa0999490:CDS
AAATTTCTTCTTGAGTGGCA+AGG 0.549157 6_4:-81542128 Msa0999490:CDS
CATAGAAGCGTTGATACAAA+GGG 0.554281 6_4:-81539990 Msa0999490:CDS
TGTCTGACTGTACGACTGCA+CGG 0.558326 6_4:-81539596 Msa0999490:three_prime_UTR
TACAGATCCAAGAAGCAGAA+GGG 0.558979 6_4:-81540336 Msa0999490:intron
TGGCAAACTCAATTCATGAC+AGG 0.567570 6_4:+81539516 None:intergenic
ACTTCTGTCACTTTATCAGT+AGG 0.569855 6_4:+81540933 None:intergenic
GCACAGCTGCTTCTTGCACA+CGG 0.570174 6_4:-81541905 Msa0999490:CDS
AAGATGAACAATTTAGCAGA+AGG 0.570848 6_4:-81540061 Msa0999490:CDS
CAACTACGAAAGTGGGCGAA+AGG 0.572916 6_4:-81541104 Msa0999490:CDS
TGTTCCAGGATTCCCAAGAA+AGG 0.574363 6_4:+81541013 None:intergenic
TAATGCAGATTGCAGTGCTA+AGG 0.575210 6_4:-81541406 Msa0999490:CDS
GTCTGACTGTACGACTGCAC+GGG 0.576403 6_4:-81539595 Msa0999490:three_prime_UTR
GAAAATACAACTACGAAAGT+GGG 0.577609 6_4:-81541111 Msa0999490:CDS
AGATGATAAGGACTACGGGT+TGG 0.581981 6_4:-81540263 Msa0999490:CDS
TGCCAAACTGCAAGGTGTAA+AGG 0.584464 6_4:+81541477 None:intergenic
AATCAGATGATAAGGACTAC+GGG 0.585566 6_4:-81540267 Msa0999490:CDS
CCTTGGAAGATTACTTCACA+TGG 0.591386 6_4:-81540967 Msa0999490:CDS
GGAGGAGATTATGTCTGTCA+TGG 0.595950 6_4:-81540233 Msa0999490:CDS
CCATGTGAAGTAATCTTCCA+AGG 0.599879 6_4:+81540967 None:intergenic
ATACAGATCCAAGAAGCAGA+AGG 0.600619 6_4:-81540337 Msa0999490:intron
TCCCAAGAAAGGCCATATGA+GGG 0.600997 6_4:+81541024 None:intergenic
TGAGTGGCAAGGATCGGAGA+GGG 0.606868 6_4:-81542117 Msa0999490:CDS
GGAAGATTACTTCACATGGT+GGG 0.609932 6_4:-81540963 Msa0999490:CDS
TTAATGAGTGCCAAACTGCA+AGG 0.610309 6_4:+81541469 None:intergenic
CCTTGCGAAAGATGATTCAA+AGG 0.611256 6_4:+81541281 None:intergenic
ACAGATCCAAGAAGCAGAAG+GGG 0.617047 6_4:-81540335 Msa0999490:intron
ACTATCTGATCTTGATCCAA+CGG 0.620328 6_4:+81539715 None:intergenic
ATTATGTCTGTCATGGACAG+TGG 0.622054 6_4:-81540226 Msa0999490:CDS
TTTCCTGCAGAAACATGAAG+AGG 0.623173 6_4:+81542170 None:intergenic
TGGAGAAACTCCATTGCACA+TGG 0.639498 6_4:-81541954 Msa0999490:CDS
TGGAAGATTACTTCACATGG+TGG 0.641918 6_4:-81540964 Msa0999490:CDS
CGAGTAATAGCTTCTAACGA+AGG 0.650852 6_4:-81540160 Msa0999490:CDS
GACAGACATAATCTCCTCCA+AGG 0.651577 6_4:+81540237 None:intergenic
AAGTTTGCAGCTGCACAATG+AGG 0.651812 6_4:-81539541 Msa0999490:three_prime_UTR
CCAAGAAAGGCCATATGAGG+GGG 0.655438 6_4:+81541026 None:intergenic
GGTCGTTGAATCAAGATAGA+CGG 0.655746 6_4:+81539626 None:intergenic
TTGAGTGGCAAGGATCGGAG+AGG 0.667286 6_4:-81542118 Msa0999490:CDS
TGATCCAACGGCCATCAATG+TGG 0.683749 6_4:+81539727 None:intergenic
CAGATCCAAGAAGCAGAAGG+GGG 0.685878 6_4:-81540334 Msa0999490:CDS
ATAAAGTGACAGAAGTACAA+CGG 0.702697 6_4:-81540926 Msa0999490:CDS
AGTCGTACAGTCAGACACAT+CGG 0.706754 6_4:+81539602 None:intergenic
CAACGTAAGGAAACAAATGG+CGG 0.727824 6_4:-81539953 Msa0999490:CDS
AGATAGTCCAGATTTCAATG+TGG 0.735798 6_4:-81539699 Msa0999490:three_prime_UTR
CGTACAGTCAGACACATCGG+TGG 0.769904 6_4:+81539605 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATACTATTTTTTTTTTTTT+TGG - chr6_4:81542284-81542303 Msa0999490:five_prime_UTR 10.0%
!!! AATAAGTTGAAAACAAATTA+TGG - chr6_4:81539832-81539851 Msa0999490:CDS 15.0%
!!! GCATAAAAATAATTTTTGTT+TGG + chr6_4:81539960-81539979 None:intergenic 15.0%
!!! CTTAAAAATGTCATTTTTAT+TGG - chr6_4:81541083-81541102 Msa0999490:CDS 15.0%
!!! TGCTTTATTTTTTTAATTAC+TGG - chr6_4:81542137-81542156 Msa0999490:CDS 15.0%
!! TAACTAAAGGATAAATGAAA+TGG + chr6_4:81539719-81539738 None:intergenic 20.0%
!! TTCAACTATATTATTTGGAT+TGG + chr6_4:81539935-81539954 None:intergenic 20.0%
!!! TGGTTTTCAACTATATTATT+TGG + chr6_4:81539940-81539959 None:intergenic 20.0%
!!! TATATAAAGTTTGTTTCCAA+TGG - chr6_4:81539992-81540011 Msa0999490:CDS 20.0%
!!! ATTATTGCTAAATAGCTTTT+TGG - chr6_4:81540204-81540223 Msa0999490:CDS 20.0%
!!! AATGAATTTGGTTTTAAGAT+TGG + chr6_4:81540894-81540913 None:intergenic 20.0%
!! ATAAAGTTGACAAAAAATAG+TGG + chr6_4:81540951-81540970 None:intergenic 20.0%
!! AAAATTGTAGTAATATTTGC+AGG - chr6_4:81541356-81541375 Msa0999490:intron 20.0%
!!! TAATTCTTTCTTTTAGTTTG+TGG - chr6_4:81542207-81542226 Msa0999490:exon 20.0%
! TAGTTAAATTTCTTCTTGAG+TGG - chr6_4:81539425-81539444 Msa0999490:three_prime_UTR 25.0%
! AACTTCAGTAGAAAATTTGA+TGG + chr6_4:81539498-81539517 None:intergenic 25.0%
! AAGCATAACACTTATCATAA+TGG - chr6_4:81539753-81539772 Msa0999490:three_prime_UTR 25.0%
! TAAAATGGATGAACTTGAAA+AGG - chr6_4:81540393-81540412 Msa0999490:intron 25.0%
! AAGGAACTTTCAAATATTGT+AGG - chr6_4:81540412-81540431 Msa0999490:intron 25.0%
! AATACTGAATTGAACCTTAT+CGG - chr6_4:81540832-81540851 Msa0999490:intron 25.0%
! ATCTCTAATGTGTATCTATT+TGG - chr6_4:81540860-81540879 Msa0999490:intron 25.0%
! GTGATTCATTAGAATGAATT+TGG + chr6_4:81540906-81540925 None:intergenic 25.0%
!! TTGAATCTTGAATCATTTTG+TGG - chr6_4:81541009-81541028 Msa0999490:CDS 25.0%
! TTGGATCTGTATAAATAACA+TGG + chr6_4:81541213-81541232 None:intergenic 25.0%
! GATTAATGCAAGAGAAAATT+TGG - chr6_4:81541646-81541665 Msa0999490:intron 25.0%
!!! TAGTTTTGACTGTATAGATA+CGG - chr6_4:81541679-81541698 Msa0999490:intron 25.0%
! TTCTAAATCTTCCAATGTAA+TGG + chr6_4:81541717-81541736 None:intergenic 25.0%
!!! TTTTGGATTTTAGAGGAATT+CGG - chr6_4:81541785-81541804 Msa0999490:intron 25.0%
! AAAATACTGAGCTTAAAATC+TGG - chr6_4:81541908-81541927 Msa0999490:CDS 25.0%
! CATACAAAGAATACAATGTA+AGG + chr6_4:81542163-81542182 None:intergenic 25.0%
! GTATGTTATTCATTCTTATG+AGG - chr6_4:81542178-81542197 Msa0999490:CDS 25.0%
! AAACCTGTTTTATATGCAGA+TGG - chr6_4:81539357-81539376 Msa0999490:three_prime_UTR 30.0%
TACAATTATGAGACAATGAC+TGG + chr6_4:81539555-81539574 None:intergenic 30.0%
CTAAAGGATAAATGAAATGG+CGG + chr6_4:81539716-81539735 None:intergenic 30.0%
GACTATAAGCTCTTAACTAA+AGG + chr6_4:81539732-81539751 None:intergenic 30.0%
AACTTAGGGAACTATTAAAC+TGG - chr6_4:81540314-81540333 Msa0999490:CDS 30.0%
!!! AAGTTCATCCATTTTAGCTT+TGG + chr6_4:81540389-81540408 None:intergenic 30.0%
TGAAAATACAACTACGAAAG+TGG - chr6_4:81540446-81540465 Msa0999490:intron 30.0%
GAAAATACAACTACGAAAGT+GGG - chr6_4:81540447-81540466 Msa0999490:intron 30.0%
! ATAAAGTGACAGAAGTACAA+CGG - chr6_4:81540632-81540651 Msa0999490:intron 30.0%
TGAAATTCCTTTACTCTTAC+AGG - chr6_4:81540724-81540743 Msa0999490:intron 30.0%
ATTAGAGATTCATACCGATA+AGG + chr6_4:81540849-81540868 None:intergenic 30.0%
AATGACGAAAGAAAAGTTCT+TGG + chr6_4:81540978-81540997 None:intergenic 30.0%
TTATCATCTGATTTCTGCAT+TGG + chr6_4:81541284-81541303 None:intergenic 30.0%
AATGCAGAAATCAGATGATA+AGG - chr6_4:81541283-81541302 Msa0999490:CDS 30.0%
AAATCAGATGATAAGGACTA+CGG - chr6_4:81541290-81541309 Msa0999490:CDS 30.0%
AAGATGAACAATTTAGCAGA+AGG - chr6_4:81541497-81541516 Msa0999490:intron 30.0%
!!! TGTTTCCTTACGTTGTTTTT+CGG + chr6_4:81541600-81541619 None:intergenic 30.0%
AAACAACGTAAGGAAACAAA+TGG - chr6_4:81541602-81541621 Msa0999490:intron 30.0%
TTCTCTTGCATTAATCATCA+TGG + chr6_4:81541642-81541661 None:intergenic 30.0%
TACAGTCAAAACTAAGTCTT+AGG + chr6_4:81541673-81541692 None:intergenic 30.0%
AGAACTTCTTACCATTACAT+TGG - chr6_4:81541703-81541722 Msa0999490:intron 30.0%
!! TTAGGTGTTTTGGATTTTAG+AGG - chr6_4:81541778-81541797 Msa0999490:intron 30.0%
TCAAAATCCACATTGAAATC+TGG + chr6_4:81541869-81541888 None:intergenic 30.0%
CTTCATGCTTATCTTACTCT+AGG - chr6_4:81539300-81539319 Msa0999490:three_prime_UTR 35.0%
! ATGTTTCTGCAGGAAACAAT+AGG - chr6_4:81539395-81539414 Msa0999490:three_prime_UTR 35.0%
! TGTTTCTGCAGGAAACAATA+GGG - chr6_4:81539396-81539415 Msa0999490:three_prime_UTR 35.0%
AAATTTCTTCTTGAGTGGCA+AGG - chr6_4:81539430-81539449 Msa0999490:three_prime_UTR 35.0%
TGAGATAGAGGCTAAGAATA+TGG - chr6_4:81539465-81539484 Msa0999490:three_prime_UTR 35.0%
ATAGAGGCTAAGAATATGGT+AGG - chr6_4:81539469-81539488 Msa0999490:three_prime_UTR 35.0%
! ACTGTTTTCACAAGTCATCT+TGG - chr6_4:81539797-81539816 Msa0999490:exon 35.0%
AAGCATTTGTGAGTCTGTTA+GGG + chr6_4:81539887-81539906 None:intergenic 35.0%
TAAGCATTTGTGAGTCTGTT+AGG + chr6_4:81539888-81539907 None:intergenic 35.0%
! AATGCCTTTTTCTTGCAGAA+TGG - chr6_4:81540051-81540070 Msa0999490:CDS 35.0%
CATACCATTCTGCAAGAAAA+AGG + chr6_4:81540058-81540077 None:intergenic 35.0%
!! ATAGCTTTTTGGTCAAATCG+TGG - chr6_4:81540215-81540234 Msa0999490:CDS 35.0%
CCCTAAGTTTATCATTTCCA+GGG + chr6_4:81540303-81540322 None:intergenic 35.0%
CCCTGGAAATGATAAACTTA+GGG - chr6_4:81540300-81540319 Msa0999490:CDS 35.0%
TCCCTAAGTTTATCATTTCC+AGG + chr6_4:81540304-81540323 None:intergenic 35.0%
!! ACTTCTGTCACTTTATCAGT+AGG + chr6_4:81540628-81540647 None:intergenic 35.0%
AATGTAGCCTGTAAGAGTAA+AGG + chr6_4:81540734-81540753 None:intergenic 35.0%
TTACAGGCTACATTTGCTTT+CGG - chr6_4:81540740-81540759 Msa0999490:intron 35.0%
! GAAACTAGACTTGTTTGCAT+TGG - chr6_4:81540768-81540787 Msa0999490:intron 35.0%
TTGACTGTACTAAGTTTAGG+CGG + chr6_4:81540928-81540947 None:intergenic 35.0%
TGGTTGACTGTACTAAGTTT+AGG + chr6_4:81540931-81540950 None:intergenic 35.0%
AATCAGATGATAAGGACTAC+GGG - chr6_4:81541291-81541310 Msa0999490:CDS 35.0%
GAAGCTATTACTCGTTTCAT+TGG + chr6_4:81541392-81541411 None:intergenic 35.0%
!!! CGATTTTAGTTCAACAGAAC+TGG - chr6_4:81541457-81541476 Msa0999490:CDS 35.0%
!! CTCTATGGATTTAAGTTGCA+TGG - chr6_4:81541527-81541546 Msa0999490:intron 35.0%
!! CATAGAAGCGTTGATACAAA+GGG - chr6_4:81541568-81541587 Msa0999490:intron 35.0%
AACAACCGAAAAACAACGTA+AGG - chr6_4:81541592-81541611 Msa0999490:intron 35.0%
ACATTGGAAGATTTAGAAGC+AGG - chr6_4:81541719-81541738 Msa0999490:intron 35.0%
AACACCTAATTGCCAAAGAA+TGG + chr6_4:81541767-81541786 None:intergenic 35.0%
! TCTTTGGCAATTAGGTGTTT+TGG - chr6_4:81541768-81541787 Msa0999490:intron 35.0%
ACTATCTGATCTTGATCCAA+CGG + chr6_4:81541846-81541865 None:intergenic 35.0%
AGATAGTCCAGATTTCAATG+TGG - chr6_4:81541859-81541878 Msa0999490:intron 35.0%
TGAATTGAGTTTGCCACAAT+AGG - chr6_4:81542049-81542068 Msa0999490:intron 35.0%
AAAGAAGGATTCACCTATTG+TGG + chr6_4:81542065-81542084 None:intergenic 35.0%
ACAATAGGTGAATCCTTCTT+TGG - chr6_4:81542064-81542083 Msa0999490:intron 35.0%
CAATAGGTGAATCCTTCTTT+GGG - chr6_4:81542065-81542084 Msa0999490:intron 35.0%
GTGCCATCTGCATATAAAAC+AGG + chr6_4:81539363-81539382 None:intergenic 40.0%
TTTCCTGCAGAAACATGAAG+AGG + chr6_4:81539391-81539410 None:intergenic 40.0%
TTTGTATCACGTCTGCAGTA+TGG - chr6_4:81539584-81539603 Msa0999490:three_prime_UTR 40.0%
! GTGTTAAGGTGTCATACCAT+TGG + chr6_4:81540011-81540030 None:intergenic 40.0%
TTAATGAGTGCCAAACTGCA+AGG + chr6_4:81540092-81540111 None:intergenic 40.0%
TAATGCAGATTGCAGTGCTA+AGG - chr6_4:81540152-81540171 Msa0999490:CDS 40.0%
GCTTAAAGCCTATGAATTGC+AGG - chr6_4:81540243-81540262 Msa0999490:CDS 40.0%
TAAAGCCTATGAATTGCAGG+AGG - chr6_4:81540246-81540265 Msa0999490:CDS 40.0%
AAAGCCTATGAATTGCAGGA+GGG - chr6_4:81540247-81540266 Msa0999490:CDS 40.0%
CCTTGCGAAAGATGATTCAA+AGG + chr6_4:81540280-81540299 None:intergenic 40.0%
CCTTTGAATCATCTTTCGCA+AGG - chr6_4:81540277-81540296 Msa0999490:CDS 40.0%
GCCCTGGAAATGATAAACTT+AGG - chr6_4:81540299-81540318 Msa0999490:CDS 40.0%
CAGCGAAACCAAAGCTAAAA+TGG - chr6_4:81540378-81540397 Msa0999490:intron 40.0%
TCCTGGAACAGGTAAAACTA+TGG - chr6_4:81540558-81540577 Msa0999490:intron 40.0%
!! ACCATAGTTTTACCTGTTCC+AGG + chr6_4:81540562-81540581 None:intergenic 40.0%
CCATGTGAAGTAATCTTCCA+AGG + chr6_4:81540594-81540613 None:intergenic 40.0%
CCTTGGAAGATTACTTCACA+TGG - chr6_4:81540591-81540610 Msa0999490:intron 40.0%
TGGAAGATTACTTCACATGG+TGG - chr6_4:81540594-81540613 Msa0999490:intron 40.0%
GGAAGATTACTTCACATGGT+GGG - chr6_4:81540595-81540614 Msa0999490:intron 40.0%
GACAAACTCGCCAACTAAAT+CGG + chr6_4:81540659-81540678 None:intergenic 40.0%
GATTTAGTTGGCGAGTTTGT+CGG - chr6_4:81540658-81540677 Msa0999490:intron 40.0%
! CATACCTTCTTCCTCGTTTT+CGG + chr6_4:81540694-81540713 None:intergenic 40.0%
ATACAGATCCAAGAAGCAGA+AGG - chr6_4:81541221-81541240 Msa0999490:CDS 40.0%
TACAGATCCAAGAAGCAGAA+GGG - chr6_4:81541222-81541241 Msa0999490:CDS 40.0%
ATTATGTCTGTCATGGACAG+TGG - chr6_4:81541332-81541351 Msa0999490:intron 40.0%
CGAGTAATAGCTTCTAACGA+AGG - chr6_4:81541398-81541417 Msa0999490:intron 40.0%
CCTTTGTATCAACGCTTCTA+TGG + chr6_4:81541570-81541589 None:intergenic 40.0%
!! CCATAGAAGCGTTGATACAA+AGG - chr6_4:81541567-81541586 Msa0999490:intron 40.0%
CAACGTAAGGAAACAAATGG+CGG - chr6_4:81541605-81541624 Msa0999490:intron 40.0%
TAAGGAAACAAATGGCGGTT+TGG - chr6_4:81541610-81541629 Msa0999490:intron 40.0%
GAAGATTTAGAAGCAGGTCT+TGG - chr6_4:81541725-81541744 Msa0999490:intron 40.0%
TTAGCACAGCAACCATTCTT+TGG - chr6_4:81541752-81541771 Msa0999490:intron 40.0%
GCAACCATTCTTTGGCAATT+AGG - chr6_4:81541760-81541779 Msa0999490:intron 40.0%
AATGCAGTTAGCCACATTGA+TGG - chr6_4:81541820-81541839 Msa0999490:intron 40.0%
GGTCGTTGAATCAAGATAGA+CGG + chr6_4:81541935-81541954 None:intergenic 40.0%
TGGCAAACTCAATTCATGAC+AGG + chr6_4:81542045-81542064 None:intergenic 40.0%
CACAAAACCTTTCCCAAAGA+AGG + chr6_4:81542080-81542099 None:intergenic 40.0%
! ACACCTCTTCATGTTTCTGC+AGG - chr6_4:81539385-81539404 Msa0999490:three_prime_UTR 45.0%
TCTTCTTGAGTGGCAAGGAT+CGG - chr6_4:81539435-81539454 Msa0999490:three_prime_UTR 45.0%
TGGAGAAACTCCATTGCACA+TGG - chr6_4:81539604-81539623 Msa0999490:three_prime_UTR 45.0%
TTCTTTGCTGCCATGTGCAA+TGG + chr6_4:81539617-81539636 None:intergenic 45.0%
TTGCACATGGCAGCAAAGAA+TGG - chr6_4:81539617-81539636 Msa0999490:three_prime_UTR 45.0%
CACCTTAACACCTTGCAGTT+TGG - chr6_4:81540020-81540039 Msa0999490:CDS 45.0%
! TGCCAAACTGCAAGGTGTTA+AGG + chr6_4:81540025-81540044 None:intergenic 45.0%
TTTGTGAGTGCCAAACTGCA+AGG + chr6_4:81540033-81540052 None:intergenic 45.0%
CACCTTTACACCTTGCAGTT+TGG - chr6_4:81540079-81540098 Msa0999490:CDS 45.0%
TGCCAAACTGCAAGGTGTAA+AGG + chr6_4:81540084-81540103 None:intergenic 45.0%
CATTCCCTCCTGCAATTCAT+AGG + chr6_4:81540254-81540273 None:intergenic 45.0%
! AAGGCATGCTTTTGGATGAG+AGG - chr6_4:81540473-81540492 Msa0999490:intron 45.0%
TCCCAAGAAAGGCCATATGA+GGG + chr6_4:81540537-81540556 None:intergenic 45.0%
TTCCCAAGAAAGGCCATATG+AGG + chr6_4:81540538-81540557 None:intergenic 45.0%
TGTTCCAGGATTCCCAAGAA+AGG + chr6_4:81540548-81540567 None:intergenic 45.0%
TTTCTTGGGAATCCTGGAAC+AGG - chr6_4:81540547-81540566 Msa0999490:intron 45.0%
! GTACAACGGACCGATTTAGT+TGG - chr6_4:81540646-81540665 Msa0999490:intron 45.0%
ACAGATCCAAGAAGCAGAAG+GGG - chr6_4:81541223-81541242 Msa0999490:CDS 45.0%
AGATGATAAGGACTACGGGT+TGG - chr6_4:81541295-81541314 Msa0999490:CDS 45.0%
GACAGACATAATCTCCTCCA+AGG + chr6_4:81541324-81541343 None:intergenic 45.0%
GGAGGAGATTATGTCTGTCA+TGG - chr6_4:81541325-81541344 Msa0999490:intron 45.0%
! CTAACGAAGGCTTTTGCAGA+CGG - chr6_4:81541411-81541430 Msa0999490:CDS 45.0%
! TAACGAAGGCTTTTGCAGAC+GGG - chr6_4:81541412-81541431 Msa0999490:CDS 45.0%
!! GCAGAAGGTAGTTTGCTCTA+TGG - chr6_4:81541512-81541531 Msa0999490:intron 45.0%
AGTCGTACAGTCAGACACAT+CGG + chr6_4:81541959-81541978 None:intergenic 45.0%
AAGTTTGCAGCTGCACAATG+AGG - chr6_4:81542017-81542036 Msa0999490:intron 45.0%
! GGTGAATCCTTCTTTGGGAA+AGG - chr6_4:81542070-81542089 Msa0999490:intron 45.0%
!! ATAATTAATTAAAATACTTA+AGG + chr6_4:81542237-81542256 None:intergenic 5.0%
!!! AATTAATTATTTTACTTTTA+TGG - chr6_4:81542247-81542266 Msa0999490:five_prime_UTR 5.0%
ATCGGAGAGGGTTGAGATAG+AGG - chr6_4:81539453-81539472 Msa0999490:three_prime_UTR 50.0%
AATCATCTTTCGCAAGGCCC+TGG - chr6_4:81540283-81540302 Msa0999490:CDS 50.0%
CAACTACGAAAGTGGGCGAA+AGG - chr6_4:81540454-81540473 Msa0999490:intron 50.0%
AGAGCTCTTGGTCTGCATGT+TGG - chr6_4:81540499-81540518 Msa0999490:intron 50.0%
CCAAGAAAGGCCATATGAGG+GGG + chr6_4:81540535-81540554 None:intergenic 50.0%
CCCAAGAAAGGCCATATGAG+GGG + chr6_4:81540536-81540555 None:intergenic 50.0%
CCCCTCATATGGCCTTTCTT+GGG - chr6_4:81540533-81540552 Msa0999490:intron 50.0%
ATGGCCTTTCTTGGGAATCC+TGG - chr6_4:81540541-81540560 Msa0999490:intron 50.0%
ACTATGGTAGCACGCATCCT+TGG - chr6_4:81540574-81540593 Msa0999490:intron 50.0%
! CTTCTTCCTCGTTTTCGGAC+CGG + chr6_4:81540689-81540708 None:intergenic 50.0%
CAGATCCAAGAAGCAGAAGG+GGG - chr6_4:81541224-81541243 Msa0999490:CDS 50.0%
GAATTCCCCCTTCTGCTTCT+TGG + chr6_4:81541232-81541251 None:intergenic 50.0%
! TTAGCCACATTGATGGCCGT+TGG - chr6_4:81541827-81541846 Msa0999490:intron 50.0%
TGATCCAACGGCCATCAATG+TGG + chr6_4:81541834-81541853 None:intergenic 50.0%
TGTCTGACTGTACGACTGCA+CGG - chr6_4:81541962-81541981 Msa0999490:intron 50.0%
TTGAGTGGCAAGGATCGGAG+AGG - chr6_4:81539440-81539459 Msa0999490:three_prime_UTR 55.0%
TGAGTGGCAAGGATCGGAGA+GGG - chr6_4:81539441-81539460 Msa0999490:three_prime_UTR 55.0%
GCACAGCTGCTTCTTGCACA+CGG - chr6_4:81539653-81539672 Msa0999490:three_prime_UTR 55.0%
!! GTGGGCGAAAGGCATGCTTT+TGG - chr6_4:81540465-81540484 Msa0999490:intron 55.0%
CACAAGACGACCCCCTCATA+TGG - chr6_4:81540522-81540541 Msa0999490:intron 55.0%
CCCCCTCATATGGCCTTTCT+TGG - chr6_4:81540532-81540551 Msa0999490:intron 55.0%
GGCGAGTTTGTCGGTCATAC+CGG - chr6_4:81540667-81540686 Msa0999490:intron 55.0%
GTCATACCGGTCCGAAAACG+AGG - chr6_4:81540680-81540699 Msa0999490:intron 55.0%
CGGTCCGAAAACGAGGAAGA+AGG - chr6_4:81540687-81540706 Msa0999490:intron 55.0%
CGTACAGTCAGACACATCGG+TGG + chr6_4:81541956-81541975 None:intergenic 55.0%
GTCTGACTGTACGACTGCAC+GGG - chr6_4:81541963-81541982 Msa0999490:intron 55.0%
! GATGAGAGGCGCAGAGCTCT+TGG - chr6_4:81540487-81540506 Msa0999490:intron 60.0%
!! GGACTACGGGTTGGAAGCCT+TGG - chr6_4:81541304-81541323 Msa0999490:intron 60.0%
!! CTACGGGTTGGAAGCCTTGG+AGG - chr6_4:81541307-81541326 Msa0999490:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81539257 81542323 81539257 ID=Msa0999490;Name=Msa0999490
chr6_4 mRNA 81539257 81542323 81539257 ID=Msa0999490-mRNA-1;Parent=Msa0999490;Name=Msa0999490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=113|1|1|1|1|1|5|542|441
chr6_4 exon 81539257 81540353 81539257 ID=Msa0999490-mRNA-1:exon:15576;Parent=Msa0999490-mRNA-1
chr6_4 exon 81540872 81541315 81540872 ID=Msa0999490-mRNA-1:exon:15575;Parent=Msa0999490-mRNA-1
chr6_4 exon 81541401 81541511 81541401 ID=Msa0999490-mRNA-1:exon:15574;Parent=Msa0999490-mRNA-1
chr6_4 exon 81541880 81541978 81541880 ID=Msa0999490-mRNA-1:exon:15573;Parent=Msa0999490-mRNA-1
chr6_4 exon 81542094 81542323 81542094 ID=Msa0999490-mRNA-1:exon:15572;Parent=Msa0999490-mRNA-1
chr6_4 five_prime_UTR 81542211 81542323 81542211 ID=Msa0999490-mRNA-1:five_prime_utr;Parent=Msa0999490-mRNA-1
chr6_4 CDS 81542094 81542210 81542094 ID=Msa0999490-mRNA-1:cds;Parent=Msa0999490-mRNA-1
chr6_4 CDS 81541880 81541978 81541880 ID=Msa0999490-mRNA-1:cds;Parent=Msa0999490-mRNA-1
chr6_4 CDS 81541401 81541511 81541401 ID=Msa0999490-mRNA-1:cds;Parent=Msa0999490-mRNA-1
chr6_4 CDS 81540872 81541315 81540872 ID=Msa0999490-mRNA-1:cds;Parent=Msa0999490-mRNA-1
chr6_4 CDS 81539799 81540353 81539799 ID=Msa0999490-mRNA-1:cds;Parent=Msa0999490-mRNA-1
chr6_4 three_prime_UTR 81539257 81539798 81539257 ID=Msa0999490-mRNA-1:three_prime_utr;Parent=Msa0999490-mRNA-1
Gene Sequence

>Msa0999490

ATGCAGATGGCACACACACCTCTTCATGTTTCTGCAGGAAACAATAGGGCTGACATAGTTAAATTTCTTCTTGAGTGGCAAGGATCGGAGAGGGTTGAGATAGAGGCTAAGAATATGTATGGAGAAACTCCATTGCACATGGCAGCAAAGAATGGATGTAGTGAAGCTGCACAGCTGCTTCTTGCACACGGCGCTATTATTGAAGCTAGAGCAAATAATGGTATGACACCTTTACACCTTGCAGTTTGGCACTCATTAAGAGCAGAAGAATTCTTAACTGTGAAAACATTGCTTGAGCATAATGCAGATTGCAGTGCTAAGGACAATGAGGGAATGACGCCTTTGAATCATCTTTCGCAAGGCCCTGGAAATGATAAACTTAGGGAACTATTAAACTGGCATCTTGAAGAGCAGAGAAAGAAGCGAGCTATTAAAGCGTGCAGCGAAACCAAAGCTAAAATGGATGAACTTGAAAAGGAACTTTCAAATATTGTAGGTCTTAATGATCTGAAAATACAACTACGAAAGTGGGCGAAAGGCATGCTTTTGGATGAGAGGCGCAGAGCTCTTGGTCTGCATGTTGGCACAAGACGACCCCCTCATATGGCCTTTCTTGGGAATCCTGGAACAGGTAAAACTATGGTAGCACGCATCCTTGGAAGATTACTTCACATGGTGGGAATTCTACCTACTGATAAAGTGACAGAAGTACAACGGACCGATTTAGTTGGCGAGTTTGTCGGTCATACCGGTCCGAAAACGAGGAAGAAGATCCAAGAAGCAGAAGGGGGAATTCTTTTTGTCGACGAAGCTTATAGACTGATACCAATGCAGAAATCAGATGATAAGGACTACGGGTTGGAAGCCTTGGAGGAGATTATGTCTGTCATGGACAGTGGAAAAATTGTAGTAATATTTGCAGGCTACAGCGAACCAATGAAACGAGTAATAGCTTCTAACGAAGGCTTTTGCAGACGGGTGACTAAGTTTTTTCATTTTATCGATTTTAGTTCAACAGAACTGGCAACAATTCTTCACATCAAGATGAACAATTTAGCAGAAGGTAGTTTGCTCTATGGATTTAAGTTGCATGGCAATTGCAGTATAAAAGCCATAGAAGCGTTGATACAAAGGGAAACAACCGAAAAACAACGTAAGGAAACAAATGGCGGTTTGGTAGATACCATGATGATTAATGCAAGAGAAAATTTGGACCTAAGACTTAGTTTTGACTGTATAGATACGGAAGAACTTCTTACCATTACATTGGAAGATTTAGAAGCAGGTCTTGGCTTTTTAGCACAGCAACCATTCTTTGGCAATTAG
Protein sequence

>Msa0999490

MQMAHTPLHVSAGNNRADIVKFLLEWQGSERVEIEAKNMYGETPLHMAAKNGCSEAAQLLLAHGAIIEARANNGMTPLHLAVWHSLRAEEFLTVKTLLEHNADCSAKDNEGMTPLNHLSQGPGNDKLRELLNWHLEEQRKKRAIKACSETKAKMDELEKELSNIVGLNDLKIQLRKWAKGMLLDERRRALGLHVGTRRPPHMAFLGNPGTGKTMVARILGRLLHMVGILPTDKVTEVQRTDLVGEFVGHTGPKTRKKIQEAEGGILFVDEAYRLIPMQKSDDKDYGLEALEEIMSVMDSGKIVVIFAGYSEPMKRVIASNEGFCRRVTKFFHFIDFSSTELATILHIKMNNLAEGSLLYGFKLHGNCSIKAIEALIQRETTEKQRKETNGGLVDTMMINARENLDLRLSFDCIDTEELLTITLEDLEAGLGFLAQQPFFGN*