AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999550 MtrunA17_Chr6g0485491 56.579 152 66 0 65 216 4 155 2.99e-56 176
Msa0999550 MtrunA17_Chr6g0485481 63.910 133 47 1 23 154 1 133 6.50e-56 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999550 AT1G79310.1 27.222 180 112 6 8 172 2 177 8.97e-13 67.0

Find 46 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCTGACCATATAATATT+TGG 0.148969 6_4:-81619185 None:intergenic
AAGATGAAAATATTGATTAT+TGG 0.166905 6_4:+81618634 Msa0999550:CDS
GACTGACTCTTCATCCCTTT+TGG 0.233722 6_4:-81619070 None:intergenic
ACTATTAAAGCTCTCTTTGT+AGG 0.282700 6_4:-81618528 None:intergenic
AGAGTCAGTCAAAGGTTCTT+TGG 0.292750 6_4:+81619083 Msa0999550:CDS
AGTATCACCTGGCTTTGATA+TGG 0.372210 6_4:-81618733 None:intergenic
GAGTCAGTCAAAGGTTCTTT+GGG 0.376370 6_4:+81619084 Msa0999550:CDS
AATTCATCATGCGTTATTCC+AGG 0.382392 6_4:-81619150 None:intergenic
CAAAGGATCCAAATATTATA+TGG 0.383489 6_4:+81619177 Msa0999550:CDS
TTCTATTCACATCTTCCAAA+AGG 0.390556 6_4:+81619055 Msa0999550:CDS
TCTTGACGCCGTCAGCCATT+TGG 0.395579 6_4:-81618969 None:intergenic
GCCAATGGAACTCCTTTACT+TGG 0.433502 6_4:+81618819 Msa0999550:CDS
TTCATGATTGATGCTTGTTA+CGG 0.446394 6_4:+81618997 Msa0999550:CDS
CTCATCAGTGGCACAAAGGT+CGG 0.466093 6_4:-81619223 None:intergenic
ATTGATGCTTGTTACGGAGG+GGG 0.492034 6_4:+81619003 Msa0999550:CDS
TCATCAGTGGCACAAAGGTC+GGG 0.493995 6_4:-81619222 None:intergenic
TGATTGATGCTTGTTACGGA+GGG 0.498805 6_4:+81619001 Msa0999550:CDS
AGTTACCTTGTTGCAGCCAA+TGG 0.499386 6_4:+81618804 Msa0999550:CDS
TCTATTCACATCTTCCAAAA+GGG 0.515999 6_4:+81619056 Msa0999550:CDS
TTGAGGAAGTTACCAAGTAA+AGG 0.533780 6_4:-81618831 None:intergenic
ACCAAGTAAAGGAGTTCCAT+TGG 0.537259 6_4:-81618820 None:intergenic
TTAATTACCATATCAAAGCC+AGG 0.542624 6_4:+81618726 Msa0999550:CDS
GATATAAACGATGGAGGACC+TGG 0.555464 6_4:+81618660 Msa0999550:CDS
ATGATTGATGCTTGTTACGG+AGG 0.558998 6_4:+81619000 Msa0999550:CDS
ATGTGAATAGAACAACTTTG+TGG 0.564614 6_4:-81619044 None:intergenic
CAAATATTATATGGTCAGCA+TGG 0.570578 6_4:+81619186 Msa0999550:CDS
GTGGCACAAAGGTCGGGTAG+TGG 0.571496 6_4:-81619216 None:intergenic
CGCATGATGAATTGATACAA+AGG 0.574943 6_4:+81619160 Msa0999550:CDS
TTGATGCTTGTTACGGAGGG+GGG 0.576592 6_4:+81619004 Msa0999550:CDS
ATTCATCATGCGTTATTCCA+GGG 0.587040 6_4:-81619149 None:intergenic
TCGGAATATGTATTGAGTGT+AGG 0.587606 6_4:-81618570 None:intergenic
GATTGATGCTTGTTACGGAG+GGG 0.589936 6_4:+81619002 Msa0999550:CDS
TAGAATCAAGTTAAGAGAGT+GGG 0.593682 6_4:+81619270 Msa0999550:exon
AGGGATGAAGAGTCAGTCAA+AGG 0.601689 6_4:+81619075 Msa0999550:CDS
GATTGTGTTAATGATTACCC+TGG 0.604085 6_4:+81619132 Msa0999550:CDS
CATGGTAACTATCAACAAAG+AGG 0.608307 6_4:+81618777 Msa0999550:CDS
AAAAGTTTGCTAATAAACCG+TGG 0.617749 6_4:+81618606 Msa0999550:CDS
ATAGAATCAAGTTAAGAGAG+TGG 0.627109 6_4:+81619269 Msa0999550:exon
ATGGTAACTATCAACAAAGA+GGG 0.636257 6_4:+81618778 Msa0999550:CDS
GAGTTCCATTGGCTGCAACA+AGG 0.637602 6_4:-81618809 None:intergenic
ACACTCAATACATATTCCGA+TGG 0.646069 6_4:+81618573 Msa0999550:CDS
GTGTTAGACCAAATGGCTGA+CGG 0.654978 6_4:+81618961 Msa0999550:CDS
TCAGCATCAATAAGGAAACC+AGG 0.656233 6_4:-81618678 None:intergenic
TATTGGCAAGATATAAACGA+TGG 0.656279 6_4:+81618651 Msa0999550:CDS
TTATCTCATCAGTGGCACAA+AGG 0.659996 6_4:-81619227 None:intergenic
TGGCAAGATATAAACGATGG+AGG 0.672578 6_4:+81618654 Msa0999550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATGAAAATATTGATTAT+TGG + chr6_4:81618634-81618653 Msa0999550:CDS 15.0%
!!! AAGATGAAAATATTGATTAT+TGG + chr6_4:81618634-81618653 Msa0999550:CDS 15.0%
!!! TATTTTCATCTTTAAATCCA+CGG - chr6_4:81618626-81618645 None:intergenic 20.0%
!!! TATTTTCATCTTTAAATCCA+CGG - chr6_4:81618626-81618645 None:intergenic 20.0%
!! TGAAAAAATAAAGTATCACC+TGG - chr6_4:81618747-81618766 None:intergenic 25.0%
!!! ACTTTATTTTTTCACTTCAG+TGG + chr6_4:81618753-81618772 Msa0999550:CDS 25.0%
!!! TAAAAAAGTGTTAGACCAAA+TGG + chr6_4:81618954-81618973 Msa0999550:CDS 25.0%
! CAAAGGATCCAAATATTATA+TGG + chr6_4:81619177-81619196 Msa0999550:CDS 25.0%
!!! AGAATTTTTTATCTCATCAG+TGG - chr6_4:81619238-81619257 None:intergenic 25.0%
!! TGAAAAAATAAAGTATCACC+TGG - chr6_4:81618747-81618766 None:intergenic 25.0%
!!! ACTTTATTTTTTCACTTCAG+TGG + chr6_4:81618753-81618772 Msa0999550:CDS 25.0%
!!! TAAAAAAGTGTTAGACCAAA+TGG + chr6_4:81618954-81618973 Msa0999550:CDS 25.0%
! CAAAGGATCCAAATATTATA+TGG + chr6_4:81619177-81619196 Msa0999550:CDS 25.0%
!!! AGAATTTTTTATCTCATCAG+TGG - chr6_4:81619238-81619257 None:intergenic 25.0%
ACTATTAAAGCTCTCTTTGT+AGG - chr6_4:81618531-81618550 None:intergenic 30.0%
!! AAAAGTTTGCTAATAAACCG+TGG + chr6_4:81618606-81618625 Msa0999550:CDS 30.0%
TATTGGCAAGATATAAACGA+TGG + chr6_4:81618651-81618670 Msa0999550:CDS 30.0%
! GTATTTTGTCAGCATCAATA+AGG - chr6_4:81618689-81618708 None:intergenic 30.0%
TTAATTACCATATCAAAGCC+AGG + chr6_4:81618726-81618745 Msa0999550:CDS 30.0%
ATGGTAACTATCAACAAAGA+GGG + chr6_4:81618778-81618797 Msa0999550:CDS 30.0%
TATTTGTGATTCTCAATGCA+TGG + chr6_4:81618882-81618901 Msa0999550:intron 30.0%
! TTCATGATTGATGCTTGTTA+CGG + chr6_4:81618997-81619016 Msa0999550:CDS 30.0%
ATGTGAATAGAACAACTTTG+TGG - chr6_4:81619047-81619066 None:intergenic 30.0%
TTCTATTCACATCTTCCAAA+AGG + chr6_4:81619055-81619074 Msa0999550:CDS 30.0%
TCTATTCACATCTTCCAAAA+GGG + chr6_4:81619056-81619075 Msa0999550:CDS 30.0%
CATGCTGACCATATAATATT+TGG - chr6_4:81619188-81619207 None:intergenic 30.0%
CAAATATTATATGGTCAGCA+TGG + chr6_4:81619186-81619205 Msa0999550:CDS 30.0%
ATAGAATCAAGTTAAGAGAG+TGG + chr6_4:81619269-81619288 Msa0999550:exon 30.0%
TAGAATCAAGTTAAGAGAGT+GGG + chr6_4:81619270-81619289 Msa0999550:exon 30.0%
ACTATTAAAGCTCTCTTTGT+AGG - chr6_4:81618531-81618550 None:intergenic 30.0%
!! AAAAGTTTGCTAATAAACCG+TGG + chr6_4:81618606-81618625 Msa0999550:CDS 30.0%
TATTGGCAAGATATAAACGA+TGG + chr6_4:81618651-81618670 Msa0999550:CDS 30.0%
! GTATTTTGTCAGCATCAATA+AGG - chr6_4:81618689-81618708 None:intergenic 30.0%
TTAATTACCATATCAAAGCC+AGG + chr6_4:81618726-81618745 Msa0999550:CDS 30.0%
ATGGTAACTATCAACAAAGA+GGG + chr6_4:81618778-81618797 Msa0999550:CDS 30.0%
TATTTGTGATTCTCAATGCA+TGG + chr6_4:81618882-81618901 Msa0999550:intron 30.0%
! TTCATGATTGATGCTTGTTA+CGG + chr6_4:81618997-81619016 Msa0999550:CDS 30.0%
ATGTGAATAGAACAACTTTG+TGG - chr6_4:81619047-81619066 None:intergenic 30.0%
TTCTATTCACATCTTCCAAA+AGG + chr6_4:81619055-81619074 Msa0999550:CDS 30.0%
TCTATTCACATCTTCCAAAA+GGG + chr6_4:81619056-81619075 Msa0999550:CDS 30.0%
CATGCTGACCATATAATATT+TGG - chr6_4:81619188-81619207 None:intergenic 30.0%
CAAATATTATATGGTCAGCA+TGG + chr6_4:81619186-81619205 Msa0999550:CDS 30.0%
ATAGAATCAAGTTAAGAGAG+TGG + chr6_4:81619269-81619288 Msa0999550:exon 30.0%
TAGAATCAAGTTAAGAGAGT+GGG + chr6_4:81619270-81619289 Msa0999550:exon 30.0%
TCGGAATATGTATTGAGTGT+AGG - chr6_4:81618573-81618592 None:intergenic 35.0%
ACACTCAATACATATTCCGA+TGG + chr6_4:81618573-81618592 Msa0999550:CDS 35.0%
! ACTTTTTATCTTCGCACCAT+CGG - chr6_4:81618592-81618611 None:intergenic 35.0%
!!! TTTTTTCACTTCAGTGGACA+TGG + chr6_4:81618759-81618778 Msa0999550:CDS 35.0%
CATGGTAACTATCAACAAAG+AGG + chr6_4:81618777-81618796 Msa0999550:CDS 35.0%
TTGAGGAAGTTACCAAGTAA+AGG - chr6_4:81618834-81618853 None:intergenic 35.0%
TATTAGCATGAGATGTGTTG+AGG - chr6_4:81618851-81618870 None:intergenic 35.0%
GATTGTGTTAATGATTACCC+TGG + chr6_4:81619132-81619151 Msa0999550:CDS 35.0%
ATTCATCATGCGTTATTCCA+GGG - chr6_4:81619152-81619171 None:intergenic 35.0%
AATTCATCATGCGTTATTCC+AGG - chr6_4:81619153-81619172 None:intergenic 35.0%
CGCATGATGAATTGATACAA+AGG + chr6_4:81619160-81619179 Msa0999550:CDS 35.0%
TCGGAATATGTATTGAGTGT+AGG - chr6_4:81618573-81618592 None:intergenic 35.0%
ACACTCAATACATATTCCGA+TGG + chr6_4:81618573-81618592 Msa0999550:CDS 35.0%
! ACTTTTTATCTTCGCACCAT+CGG - chr6_4:81618592-81618611 None:intergenic 35.0%
!!! TTTTTTCACTTCAGTGGACA+TGG + chr6_4:81618759-81618778 Msa0999550:CDS 35.0%
CATGGTAACTATCAACAAAG+AGG + chr6_4:81618777-81618796 Msa0999550:CDS 35.0%
TTGAGGAAGTTACCAAGTAA+AGG - chr6_4:81618834-81618853 None:intergenic 35.0%
TATTAGCATGAGATGTGTTG+AGG - chr6_4:81618851-81618870 None:intergenic 35.0%
GATTGTGTTAATGATTACCC+TGG + chr6_4:81619132-81619151 Msa0999550:CDS 35.0%
ATTCATCATGCGTTATTCCA+GGG - chr6_4:81619152-81619171 None:intergenic 35.0%
AATTCATCATGCGTTATTCC+AGG - chr6_4:81619153-81619172 None:intergenic 35.0%
CGCATGATGAATTGATACAA+AGG + chr6_4:81619160-81619179 Msa0999550:CDS 35.0%
TGGCAAGATATAAACGATGG+AGG + chr6_4:81618654-81618673 Msa0999550:CDS 40.0%
TCAGCATCAATAAGGAAACC+AGG - chr6_4:81618681-81618700 None:intergenic 40.0%
!! AGTATCACCTGGCTTTGATA+TGG - chr6_4:81618736-81618755 None:intergenic 40.0%
ACCAAGTAAAGGAGTTCCAT+TGG - chr6_4:81618823-81618842 None:intergenic 40.0%
! ATGATTGATGCTTGTTACGG+AGG + chr6_4:81619000-81619019 Msa0999550:CDS 40.0%
! TGATTGATGCTTGTTACGGA+GGG + chr6_4:81619001-81619020 Msa0999550:CDS 40.0%
AGAGTCAGTCAAAGGTTCTT+TGG + chr6_4:81619083-81619102 Msa0999550:CDS 40.0%
GAGTCAGTCAAAGGTTCTTT+GGG + chr6_4:81619084-81619103 Msa0999550:CDS 40.0%
TTATCTCATCAGTGGCACAA+AGG - chr6_4:81619230-81619249 None:intergenic 40.0%
TGGCAAGATATAAACGATGG+AGG + chr6_4:81618654-81618673 Msa0999550:CDS 40.0%
TCAGCATCAATAAGGAAACC+AGG - chr6_4:81618681-81618700 None:intergenic 40.0%
!! AGTATCACCTGGCTTTGATA+TGG - chr6_4:81618736-81618755 None:intergenic 40.0%
ACCAAGTAAAGGAGTTCCAT+TGG - chr6_4:81618823-81618842 None:intergenic 40.0%
! ATGATTGATGCTTGTTACGG+AGG + chr6_4:81619000-81619019 Msa0999550:CDS 40.0%
! TGATTGATGCTTGTTACGGA+GGG + chr6_4:81619001-81619020 Msa0999550:CDS 40.0%
AGAGTCAGTCAAAGGTTCTT+TGG + chr6_4:81619083-81619102 Msa0999550:CDS 40.0%
GAGTCAGTCAAAGGTTCTTT+GGG + chr6_4:81619084-81619103 Msa0999550:CDS 40.0%
TTATCTCATCAGTGGCACAA+AGG - chr6_4:81619230-81619249 None:intergenic 40.0%
GATATAAACGATGGAGGACC+TGG + chr6_4:81618660-81618679 Msa0999550:CDS 45.0%
AGTTACCTTGTTGCAGCCAA+TGG + chr6_4:81618804-81618823 Msa0999550:CDS 45.0%
GCCAATGGAACTCCTTTACT+TGG + chr6_4:81618819-81618838 Msa0999550:CDS 45.0%
! ATTTGCTACGTGACTTGTGC+AGG + chr6_4:81618922-81618941 Msa0999550:intron 45.0%
GTGTTAGACCAAATGGCTGA+CGG + chr6_4:81618961-81618980 Msa0999550:CDS 45.0%
! GATTGATGCTTGTTACGGAG+GGG + chr6_4:81619002-81619021 Msa0999550:CDS 45.0%
! ATTGATGCTTGTTACGGAGG+GGG + chr6_4:81619003-81619022 Msa0999550:CDS 45.0%
!! GACTGACTCTTCATCCCTTT+TGG - chr6_4:81619073-81619092 None:intergenic 45.0%
AGGGATGAAGAGTCAGTCAA+AGG + chr6_4:81619075-81619094 Msa0999550:CDS 45.0%
GATATAAACGATGGAGGACC+TGG + chr6_4:81618660-81618679 Msa0999550:CDS 45.0%
AGTTACCTTGTTGCAGCCAA+TGG + chr6_4:81618804-81618823 Msa0999550:CDS 45.0%
GCCAATGGAACTCCTTTACT+TGG + chr6_4:81618819-81618838 Msa0999550:CDS 45.0%
! ATTTGCTACGTGACTTGTGC+AGG + chr6_4:81618922-81618941 Msa0999550:intron 45.0%
GTGTTAGACCAAATGGCTGA+CGG + chr6_4:81618961-81618980 Msa0999550:CDS 45.0%
! GATTGATGCTTGTTACGGAG+GGG + chr6_4:81619002-81619021 Msa0999550:CDS 45.0%
! ATTGATGCTTGTTACGGAGG+GGG + chr6_4:81619003-81619022 Msa0999550:CDS 45.0%
!! GACTGACTCTTCATCCCTTT+TGG - chr6_4:81619073-81619092 None:intergenic 45.0%
AGGGATGAAGAGTCAGTCAA+AGG + chr6_4:81619075-81619094 Msa0999550:CDS 45.0%
! GAGTTCCATTGGCTGCAACA+AGG - chr6_4:81618812-81618831 None:intergenic 50.0%
! TTGATGCTTGTTACGGAGGG+GGG + chr6_4:81619004-81619023 Msa0999550:CDS 50.0%
TCATCAGTGGCACAAAGGTC+GGG - chr6_4:81619225-81619244 None:intergenic 50.0%
CTCATCAGTGGCACAAAGGT+CGG - chr6_4:81619226-81619245 None:intergenic 50.0%
! GAGTTCCATTGGCTGCAACA+AGG - chr6_4:81618812-81618831 None:intergenic 50.0%
! TTGATGCTTGTTACGGAGGG+GGG + chr6_4:81619004-81619023 Msa0999550:CDS 50.0%
TCATCAGTGGCACAAAGGTC+GGG - chr6_4:81619225-81619244 None:intergenic 50.0%
CTCATCAGTGGCACAAAGGT+CGG - chr6_4:81619226-81619245 None:intergenic 50.0%
TCTTGACGCCGTCAGCCATT+TGG - chr6_4:81618972-81618991 None:intergenic 55.0%
TCTTGACGCCGTCAGCCATT+TGG - chr6_4:81618972-81618991 None:intergenic 55.0%
GTGGCACAAAGGTCGGGTAG+TGG - chr6_4:81619219-81619238 None:intergenic 60.0%
GTGGCACAAAGGTCGGGTAG+TGG - chr6_4:81619219-81619238 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81618510 81619332 81618510 ID=Msa0999550;Name=Msa0999550
chr6_4 mRNA 81618510 81619332 81618510 ID=Msa0999550-mRNA-1;Parent=Msa0999550;Name=Msa0999550-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|1|0.5|1|1|1|2|60|219
chr6_4 exon 81618510 81618840 81618510 ID=Msa0999550-mRNA-1:exon:15594;Parent=Msa0999550-mRNA-1
chr6_4 exon 81618944 81619332 81618944 ID=Msa0999550-mRNA-1:exon:15595;Parent=Msa0999550-mRNA-1
chr6_4 CDS 81618510 81618840 81618510 ID=Msa0999550-mRNA-1:cds;Parent=Msa0999550-mRNA-1
chr6_4 CDS 81618944 81619272 81618944 ID=Msa0999550-mRNA-1:cds;Parent=Msa0999550-mRNA-1
chr6_4 three_prime_UTR 81619273 81619332 81619273 ID=Msa0999550-mRNA-1:three_prime_utr;Parent=Msa0999550-mRNA-1
Gene Sequence

>Msa0999550

TTGTACAGCAATAATTCTCCTACAAAGAGAGCTTTAATAGTCAATTTACAATTTCGAGAACCTACACTCAATACATATTCCGATGGTGCGAAGATAAAAAGTTTGCTAATAAACCGTGGATTTAAAGATGAAAATATTGATTATTGGCAAGATATAAACGATGGAGGACCTGGTTTCCTTATTGATGCTGACAAAATACGTAATAGATTACATGATTTAATTACCATATCAAAGCCAGGTGATACTTTATTTTTTCACTTCAGTGGACATGGTAACTATCAACAAAGAGGGAGAAGTTACCTTGTTGCAGCCAATGGAACTCCTTTACTTGGTTGTGAATTTAAAAAAGTGTTAGACCAAATGGCTGACGGCGTCAAGATAATTTTCATGATTGATGCTTGTTACGGAGGGGGGTTTATTGAACTAGCTCACCACAAAGTTGTTCTATTCACATCTTCCAAAAGGGATGAAGAGTCAGTCAAAGGTTCTTTGGGTTCTGTGTTTACGAATGCGTTTGTTGATTGTGTTAATGATTACCCTGGAATAACGCATGATGAATTGATACAAAGGATCCAAATATTATATGGTCAGCATGGAAGCAAACCACTACCCGACCTTTGTGCCACTGATGAGATAAAAAATTCTATTTTTATTCTATAG

Protein sequence

>Msa0999550

LYSNNSPTKRALIVNLQFREPTLNTYSDGAKIKSLLINRGFKDENIDYWQDINDGGPGFLIDADKIRNRLHDLITISKPGDTLFFHFSGHGNYQQRGRSYLVAANGTPLLGCEFKKVLDQMADGVKIIFMIDACYGGGFIELAHHKVVLFTSSKRDEESVKGSLGSVFTNAFVDCVNDYPGITHDELIQRIQILYGQHGSKPLPDLCATDEIKNSIFIL*