AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999610 MtrunA17_Chr1g0181621 100.000 88 0 0 12 99 55 142 2.84e-59 178
Msa0999610 MtrunA17_Chr7g0263691 100.000 88 0 0 12 99 55 142 4.45e-59 177
Msa0999610 MtrunA17_Chr7g0239631 60.000 55 20 1 12 66 29 81 1.22e-15 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999610 AT5G02960.1 100.000 88 0 0 12 99 55 142 8.54e-59 177
Msa0999610 AT3G09680.1 100.000 88 0 0 12 99 55 142 1.04e-58 177

Find 48 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAGGTTGTGAAGGTCTC+TGG 0.340450 6_4:-81842439 Msa0999610:CDS
TCTCCTTCTTCTCCTTGAAA+AGG 0.352760 6_4:+81842401 None:intergenic
ATTCCACAAATCTAAGATCT+TGG 0.379899 6_4:+81842376 None:intergenic
TGTGTTGAGCTCGGAGGTTA+TGG 0.405375 6_4:+81843027 None:intergenic
TCTGTAAATGCCCACTCAAC+AGG 0.411916 6_4:+81842306 None:intergenic
CAGCTATTCGTAAGTGTGCC+AGG 0.413818 6_4:-81842754 Msa0999610:CDS
CATGCTGTGGGAGATATTCC+TGG 0.414411 6_4:-81842469 Msa0999610:CDS
CTCCTTCTTCTCCTTGAAAA+GGG 0.418297 6_4:+81842402 None:intergenic
ACACAAGGACCAAGCAGCCA+TGG 0.440627 6_4:-81843007 Msa0999610:five_prime_UTR
AAGATCTTAGATTTGTGGAA+TGG 0.444307 6_4:-81842374 Msa0999610:exon
CACTTACGAATAGCTGAGTT+GGG 0.449683 6_4:+81842761 None:intergenic
AGATCTTAGATTTGTGGAAT+GGG 0.462917 6_4:-81842373 Msa0999610:exon
TCAAGGTTGTGAAGGTCTCT+GGG 0.478339 6_4:-81842438 Msa0999610:CDS
TGGTCCTTGTGTGTTGAGCT+CGG 0.488561 6_4:+81843018 None:intergenic
CATGGGGAATACACATGGAA+TGG 0.495178 6_4:-81842989 Msa0999610:five_prime_UTR
GCTGGTTCTTCCCACGCAAA+GGG 0.497054 6_4:-81842913 Msa0999610:intron
TAGTTTAAACAACCATCGTT+GGG 0.498002 6_4:+81842689 None:intergenic
GTGTATTCCCCATGGCTGCT+TGG 0.498685 6_4:+81842998 None:intergenic
ATTGCTGGATTTGGAAGAAA+GGG 0.499669 6_4:-81842493 Msa0999610:CDS
GCAAGCTGAAGTCTCACCTT+GGG 0.501904 6_4:-81842957 Msa0999610:five_prime_UTR
GATGTAAGAGTGGTTAAGCT+TGG 0.505553 6_4:-81842875 Msa0999610:five_prime_UTR
GATTGCTGGATTTGGAAGAA+AGG 0.508603 6_4:-81842494 Msa0999610:CDS
TATTCCTGGAGTTAGATTCA+AGG 0.508883 6_4:-81842455 Msa0999610:CDS
ACACTTACGAATAGCTGAGT+TGG 0.509353 6_4:+81842760 None:intergenic
CACAAGGACCAAGCAGCCAT+GGG 0.511008 6_4:-81843006 Msa0999610:five_prime_UTR
AGGGTACAACTAATCAAGAA+TGG 0.513747 6_4:-81842734 Msa0999610:CDS
CGCAAGCTGAAGTCTCACCT+TGG 0.514161 6_4:-81842958 Msa0999610:five_prime_UTR
AAGCCAAGATCTTAGATTTG+TGG 0.520253 6_4:-81842379 Msa0999610:exon
ATGGGGAATACACATGGAAT+GGG 0.535864 6_4:-81842988 Msa0999610:five_prime_UTR
GTAGTTTAAACAACCATCGT+TGG 0.552087 6_4:+81842688 None:intergenic
CATTCCATGTGTATTCCCCA+TGG 0.562380 6_4:+81842990 None:intergenic
GTTTCTTCCACTCATTCCCA+AGG 0.565189 6_4:+81842941 None:intergenic
ACAACCATCGTTGGGAACAA+AGG 0.572957 6_4:+81842697 None:intergenic
ATTCTTGATTAGTTGTACCC+TGG 0.574592 6_4:+81842736 None:intergenic
GTTGAGCTCGGAGGTTATGG+AGG 0.583331 6_4:+81843030 None:intergenic
TGGAAGAAAGGGTCATGCTG+TGG 0.584767 6_4:-81842482 Msa0999610:CDS
GGAAGAAAGGGTCATGCTGT+GGG 0.588458 6_4:-81842481 Msa0999610:CDS
AGTTAGATTCAAGGTTGTGA+AGG 0.593952 6_4:-81842446 Msa0999610:CDS
ACAACCTTGAATCTAACTCC+AGG 0.602701 6_4:+81842451 None:intergenic
GCTGCCTTTGTTCCCAACGA+TGG 0.607412 6_4:-81842701 Msa0999610:CDS
ACCTCCGAGCTCAACACACA+AGG 0.619600 6_4:-81843022 Msa0999610:five_prime_UTR
TCCTCGAAAAGATGTAAGAG+TGG 0.631831 6_4:-81842885 Msa0999610:five_prime_UTR
AGCTATTCGTAAGTGTGCCA+GGG 0.641920 6_4:-81842753 Msa0999610:CDS
TTGAGCTCGGAGGTTATGGA+GGG 0.646209 6_4:+81843031 None:intergenic
GCAGCCATGGGGAATACACA+TGG 0.647637 6_4:-81842994 Msa0999610:five_prime_UTR
CAAGGTTGTGAAGGTCTCTG+GGG 0.680000 6_4:-81842437 Msa0999610:CDS
TCCTTGTGTGTTGAGCTCGG+AGG 0.735284 6_4:+81843021 None:intergenic
ACAAGGACCAAGCAGCCATG+GGG 0.741823 6_4:-81843005 Msa0999610:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTATTTTTATTTTT+TGG + chr6_4:81843036-81843055 None:intergenic 0.0%
!!! TTTTTTTTATTTTTATTTTT+TGG + chr6_4:81843036-81843055 None:intergenic 0.0%
!!! GATTGTTGTTTTATTTACAA+AGG - chr6_4:81842430-81842449 Msa0999610:CDS 20.0%
!!! TTTTATTTACAAAGGTATTG+AGG - chr6_4:81842438-81842457 Msa0999610:CDS 20.0%
!! AGCAAAAATCAACATAATTT+AGG + chr6_4:81842903-81842922 None:intergenic 20.0%
!!! GATTGTTGTTTTATTTACAA+AGG - chr6_4:81842430-81842449 Msa0999610:CDS 20.0%
!!! TTTTATTTACAAAGGTATTG+AGG - chr6_4:81842438-81842457 Msa0999610:CDS 20.0%
!! AGCAAAAATCAACATAATTT+AGG + chr6_4:81842903-81842922 None:intergenic 20.0%
!! TGATTTTAAACTGTTATGTC+TGG - chr6_4:81842614-81842633 Msa0999610:intron 25.0%
!!! TTTTGCATCTTGTTATTTTG+TGG - chr6_4:81842950-81842969 Msa0999610:intron 25.0%
!! TGATTTTAAACTGTTATGTC+TGG - chr6_4:81842614-81842633 Msa0999610:intron 25.0%
!!! TTTTGCATCTTGTTATTTTG+TGG - chr6_4:81842950-81842969 Msa0999610:intron 25.0%
TAGTTTAAACAACCATCGTT+GGG + chr6_4:81842544-81842563 None:intergenic 30.0%
! TCCTTGCTTGTATTTTCTAT+TGG - chr6_4:81842582-81842601 Msa0999610:intron 30.0%
ACCAATAGAAAATACAAGCA+AGG + chr6_4:81842586-81842605 None:intergenic 30.0%
! ATTCCACAAATCTAAGATCT+TGG + chr6_4:81842857-81842876 None:intergenic 30.0%
AAGATCTTAGATTTGTGGAA+TGG - chr6_4:81842856-81842875 Msa0999610:five_prime_UTR 30.0%
AGATCTTAGATTTGTGGAAT+GGG - chr6_4:81842857-81842876 Msa0999610:five_prime_UTR 30.0%
! CATTTTTCACTCTTACTCTT+AGG - chr6_4:81842978-81842997 Msa0999610:five_prime_UTR 30.0%
!! TTTTTGGAAGATTCACAAGT+AGG + chr6_4:81843020-81843039 None:intergenic 30.0%
TAGTTTAAACAACCATCGTT+GGG + chr6_4:81842544-81842563 None:intergenic 30.0%
! TCCTTGCTTGTATTTTCTAT+TGG - chr6_4:81842582-81842601 Msa0999610:intron 30.0%
ACCAATAGAAAATACAAGCA+AGG + chr6_4:81842586-81842605 None:intergenic 30.0%
! ATTCCACAAATCTAAGATCT+TGG + chr6_4:81842857-81842876 None:intergenic 30.0%
AAGATCTTAGATTTGTGGAA+TGG - chr6_4:81842856-81842875 Msa0999610:five_prime_UTR 30.0%
AGATCTTAGATTTGTGGAAT+GGG - chr6_4:81842857-81842876 Msa0999610:five_prime_UTR 30.0%
! CATTTTTCACTCTTACTCTT+AGG - chr6_4:81842978-81842997 Msa0999610:five_prime_UTR 30.0%
!! TTTTTGGAAGATTCACAAGT+AGG + chr6_4:81843020-81843039 None:intergenic 30.0%
! ATTCTTGATTAGTTGTACCC+TGG + chr6_4:81842497-81842516 None:intergenic 35.0%
AGGGTACAACTAATCAAGAA+TGG - chr6_4:81842496-81842515 Msa0999610:CDS 35.0%
GTAGTTTAAACAACCATCGT+TGG + chr6_4:81842545-81842564 None:intergenic 35.0%
ACACACAATGTCACACAATA+TGG + chr6_4:81842690-81842709 None:intergenic 35.0%
!!! GAAGTTTTGATTGCTGGATT+TGG - chr6_4:81842728-81842747 Msa0999610:CDS 35.0%
ATTGCTGGATTTGGAAGAAA+GGG - chr6_4:81842737-81842756 Msa0999610:CDS 35.0%
TATTCCTGGAGTTAGATTCA+AGG - chr6_4:81842775-81842794 Msa0999610:CDS 35.0%
AGTTAGATTCAAGGTTGTGA+AGG - chr6_4:81842784-81842803 Msa0999610:CDS 35.0%
AAGCCAAGATCTTAGATTTG+TGG - chr6_4:81842851-81842870 Msa0999610:five_prime_UTR 35.0%
! ATTTTTGCTTCCTGTTGAGT+GGG - chr6_4:81842914-81842933 Msa0999610:intron 35.0%
! ATTCTTGATTAGTTGTACCC+TGG + chr6_4:81842497-81842516 None:intergenic 35.0%
AGGGTACAACTAATCAAGAA+TGG - chr6_4:81842496-81842515 Msa0999610:CDS 35.0%
GTAGTTTAAACAACCATCGT+TGG + chr6_4:81842545-81842564 None:intergenic 35.0%
ACACACAATGTCACACAATA+TGG + chr6_4:81842690-81842709 None:intergenic 35.0%
!!! GAAGTTTTGATTGCTGGATT+TGG - chr6_4:81842728-81842747 Msa0999610:CDS 35.0%
ATTGCTGGATTTGGAAGAAA+GGG - chr6_4:81842737-81842756 Msa0999610:CDS 35.0%
TATTCCTGGAGTTAGATTCA+AGG - chr6_4:81842775-81842794 Msa0999610:CDS 35.0%
AGTTAGATTCAAGGTTGTGA+AGG - chr6_4:81842784-81842803 Msa0999610:CDS 35.0%
AAGCCAAGATCTTAGATTTG+TGG - chr6_4:81842851-81842870 Msa0999610:five_prime_UTR 35.0%
! ATTTTTGCTTCCTGTTGAGT+GGG - chr6_4:81842914-81842933 Msa0999610:intron 35.0%
ATGGGGAATACACATGGAAT+GGG - chr6_4:81842242-81842261 Msa0999610:three_prime_UTR 40.0%
TCCTCGAAAAGATGTAAGAG+TGG - chr6_4:81842345-81842364 Msa0999610:three_prime_UTR 40.0%
! ACCACTCTTACATCTTTTCG+AGG + chr6_4:81842349-81842368 None:intergenic 40.0%
GATGTAAGAGTGGTTAAGCT+TGG - chr6_4:81842355-81842374 Msa0999610:three_prime_UTR 40.0%
CACTTACGAATAGCTGAGTT+GGG + chr6_4:81842472-81842491 None:intergenic 40.0%
ACACTTACGAATAGCTGAGT+TGG + chr6_4:81842473-81842492 None:intergenic 40.0%
!!! CAGGATGAAGTTTTGATTGC+TGG - chr6_4:81842722-81842741 Msa0999610:CDS 40.0%
GATTGCTGGATTTGGAAGAA+AGG - chr6_4:81842736-81842755 Msa0999610:CDS 40.0%
ACAACCTTGAATCTAACTCC+AGG + chr6_4:81842782-81842801 None:intergenic 40.0%
CTCCTTCTTCTCCTTGAAAA+GGG + chr6_4:81842831-81842850 None:intergenic 40.0%
TCTCCTTCTTCTCCTTGAAA+AGG + chr6_4:81842832-81842851 None:intergenic 40.0%
! GATTTTTGCTTCCTGTTGAG+TGG - chr6_4:81842913-81842932 Msa0999610:intron 40.0%
ATGGGGAATACACATGGAAT+GGG - chr6_4:81842242-81842261 Msa0999610:three_prime_UTR 40.0%
TCCTCGAAAAGATGTAAGAG+TGG - chr6_4:81842345-81842364 Msa0999610:three_prime_UTR 40.0%
! ACCACTCTTACATCTTTTCG+AGG + chr6_4:81842349-81842368 None:intergenic 40.0%
GATGTAAGAGTGGTTAAGCT+TGG - chr6_4:81842355-81842374 Msa0999610:three_prime_UTR 40.0%
CACTTACGAATAGCTGAGTT+GGG + chr6_4:81842472-81842491 None:intergenic 40.0%
ACACTTACGAATAGCTGAGT+TGG + chr6_4:81842473-81842492 None:intergenic 40.0%
!!! CAGGATGAAGTTTTGATTGC+TGG - chr6_4:81842722-81842741 Msa0999610:CDS 40.0%
GATTGCTGGATTTGGAAGAA+AGG - chr6_4:81842736-81842755 Msa0999610:CDS 40.0%
ACAACCTTGAATCTAACTCC+AGG + chr6_4:81842782-81842801 None:intergenic 40.0%
CTCCTTCTTCTCCTTGAAAA+GGG + chr6_4:81842831-81842850 None:intergenic 40.0%
TCTCCTTCTTCTCCTTGAAA+AGG + chr6_4:81842832-81842851 None:intergenic 40.0%
! GATTTTTGCTTCCTGTTGAG+TGG - chr6_4:81842913-81842932 Msa0999610:intron 40.0%
CATTCCATGTGTATTCCCCA+TGG + chr6_4:81842243-81842262 None:intergenic 45.0%
CATGGGGAATACACATGGAA+TGG - chr6_4:81842241-81842260 Msa0999610:three_prime_UTR 45.0%
GTTTCTTCCACTCATTCCCA+AGG + chr6_4:81842292-81842311 None:intergenic 45.0%
! GAGTGGAAGAAACCTTTTGC+TGG - chr6_4:81842299-81842318 Msa0999610:three_prime_UTR 45.0%
AGCTATTCGTAAGTGTGCCA+GGG - chr6_4:81842477-81842496 Msa0999610:CDS 45.0%
ACAACCATCGTTGGGAACAA+AGG + chr6_4:81842536-81842555 None:intergenic 45.0%
GTGACATTGTGTGTGACTGA+TGG - chr6_4:81842696-81842715 Msa0999610:CDS 45.0%
TTCAAGGTTGTGAAGGTCTC+TGG - chr6_4:81842791-81842810 Msa0999610:CDS 45.0%
TCAAGGTTGTGAAGGTCTCT+GGG - chr6_4:81842792-81842811 Msa0999610:CDS 45.0%
! GTCTCTTCTTGCCCTTTTCA+AGG - chr6_4:81842817-81842836 Msa0999610:exon 45.0%
!! TGCCCTTTTCAAGGAGAAGA+AGG - chr6_4:81842826-81842845 Msa0999610:exon 45.0%
TCTGTAAATGCCCACTCAAC+AGG + chr6_4:81842927-81842946 None:intergenic 45.0%
CATTCCATGTGTATTCCCCA+TGG + chr6_4:81842243-81842262 None:intergenic 45.0%
CATGGGGAATACACATGGAA+TGG - chr6_4:81842241-81842260 Msa0999610:three_prime_UTR 45.0%
GTTTCTTCCACTCATTCCCA+AGG + chr6_4:81842292-81842311 None:intergenic 45.0%
! GAGTGGAAGAAACCTTTTGC+TGG - chr6_4:81842299-81842318 Msa0999610:three_prime_UTR 45.0%
AGCTATTCGTAAGTGTGCCA+GGG - chr6_4:81842477-81842496 Msa0999610:CDS 45.0%
ACAACCATCGTTGGGAACAA+AGG + chr6_4:81842536-81842555 None:intergenic 45.0%
GTGACATTGTGTGTGACTGA+TGG - chr6_4:81842696-81842715 Msa0999610:CDS 45.0%
TTCAAGGTTGTGAAGGTCTC+TGG - chr6_4:81842791-81842810 Msa0999610:CDS 45.0%
TCAAGGTTGTGAAGGTCTCT+GGG - chr6_4:81842792-81842811 Msa0999610:CDS 45.0%
! GTCTCTTCTTGCCCTTTTCA+AGG - chr6_4:81842817-81842836 Msa0999610:exon 45.0%
!! TGCCCTTTTCAAGGAGAAGA+AGG - chr6_4:81842826-81842845 Msa0999610:exon 45.0%
TCTGTAAATGCCCACTCAAC+AGG + chr6_4:81842927-81842946 None:intergenic 45.0%
TTGAGCTCGGAGGTTATGGA+GGG + chr6_4:81842202-81842221 None:intergenic 50.0%
TGTGTTGAGCTCGGAGGTTA+TGG + chr6_4:81842206-81842225 None:intergenic 50.0%
TGGTCCTTGTGTGTTGAGCT+CGG + chr6_4:81842215-81842234 None:intergenic 50.0%
GCAAGCTGAAGTCTCACCTT+GGG - chr6_4:81842273-81842292 Msa0999610:three_prime_UTR 50.0%
AGTCTCACCTTGGGAATGAG+TGG - chr6_4:81842282-81842301 Msa0999610:three_prime_UTR 50.0%
GAGGACAATTCCCTTTGCGT+GGG + chr6_4:81842330-81842349 None:intergenic 50.0%
CAGCTATTCGTAAGTGTGCC+AGG - chr6_4:81842476-81842495 Msa0999610:CDS 50.0%
TGTGTGTGACTGATGGTTGC+AGG - chr6_4:81842703-81842722 Msa0999610:CDS 50.0%
TGGAAGAAAGGGTCATGCTG+TGG - chr6_4:81842748-81842767 Msa0999610:CDS 50.0%
GGAAGAAAGGGTCATGCTGT+GGG - chr6_4:81842749-81842768 Msa0999610:CDS 50.0%
CATGCTGTGGGAGATATTCC+TGG - chr6_4:81842761-81842780 Msa0999610:CDS 50.0%
CAAGGTTGTGAAGGTCTCTG+GGG - chr6_4:81842793-81842812 Msa0999610:CDS 50.0%
TTGAGCTCGGAGGTTATGGA+GGG + chr6_4:81842202-81842221 None:intergenic 50.0%
TGTGTTGAGCTCGGAGGTTA+TGG + chr6_4:81842206-81842225 None:intergenic 50.0%
TGGTCCTTGTGTGTTGAGCT+CGG + chr6_4:81842215-81842234 None:intergenic 50.0%
GCAAGCTGAAGTCTCACCTT+GGG - chr6_4:81842273-81842292 Msa0999610:three_prime_UTR 50.0%
AGTCTCACCTTGGGAATGAG+TGG - chr6_4:81842282-81842301 Msa0999610:three_prime_UTR 50.0%
GAGGACAATTCCCTTTGCGT+GGG + chr6_4:81842330-81842349 None:intergenic 50.0%
CAGCTATTCGTAAGTGTGCC+AGG - chr6_4:81842476-81842495 Msa0999610:CDS 50.0%
TGTGTGTGACTGATGGTTGC+AGG - chr6_4:81842703-81842722 Msa0999610:CDS 50.0%
TGGAAGAAAGGGTCATGCTG+TGG - chr6_4:81842748-81842767 Msa0999610:CDS 50.0%
GGAAGAAAGGGTCATGCTGT+GGG - chr6_4:81842749-81842768 Msa0999610:CDS 50.0%
CATGCTGTGGGAGATATTCC+TGG - chr6_4:81842761-81842780 Msa0999610:CDS 50.0%
CAAGGTTGTGAAGGTCTCTG+GGG - chr6_4:81842793-81842812 Msa0999610:CDS 50.0%
GTTGAGCTCGGAGGTTATGG+AGG + chr6_4:81842203-81842222 None:intergenic 55.0%
ACCTCCGAGCTCAACACACA+AGG - chr6_4:81842208-81842227 Msa0999610:three_prime_UTR 55.0%
TCCTTGTGTGTTGAGCTCGG+AGG + chr6_4:81842212-81842231 None:intergenic 55.0%
ACACAAGGACCAAGCAGCCA+TGG - chr6_4:81842223-81842242 Msa0999610:three_prime_UTR 55.0%
CACAAGGACCAAGCAGCCAT+GGG - chr6_4:81842224-81842243 Msa0999610:three_prime_UTR 55.0%
ACAAGGACCAAGCAGCCATG+GGG - chr6_4:81842225-81842244 Msa0999610:three_prime_UTR 55.0%
GTGTATTCCCCATGGCTGCT+TGG + chr6_4:81842235-81842254 None:intergenic 55.0%
GCAGCCATGGGGAATACACA+TGG - chr6_4:81842236-81842255 Msa0999610:three_prime_UTR 55.0%
CGCAAGCTGAAGTCTCACCT+TGG - chr6_4:81842272-81842291 Msa0999610:three_prime_UTR 55.0%
GCGTGGGAAGAACCAGCAAA+AGG + chr6_4:81842314-81842333 None:intergenic 55.0%
!! TGCTGGTTCTTCCCACGCAA+AGG - chr6_4:81842316-81842335 Msa0999610:three_prime_UTR 55.0%
! GCTGGTTCTTCCCACGCAAA+GGG - chr6_4:81842317-81842336 Msa0999610:three_prime_UTR 55.0%
CGAGGACAATTCCCTTTGCG+TGG + chr6_4:81842331-81842350 None:intergenic 55.0%
GCTGCCTTTGTTCCCAACGA+TGG - chr6_4:81842529-81842548 Msa0999610:intron 55.0%
GTTGAGCTCGGAGGTTATGG+AGG + chr6_4:81842203-81842222 None:intergenic 55.0%
ACCTCCGAGCTCAACACACA+AGG - chr6_4:81842208-81842227 Msa0999610:three_prime_UTR 55.0%
TCCTTGTGTGTTGAGCTCGG+AGG + chr6_4:81842212-81842231 None:intergenic 55.0%
ACACAAGGACCAAGCAGCCA+TGG - chr6_4:81842223-81842242 Msa0999610:three_prime_UTR 55.0%
CACAAGGACCAAGCAGCCAT+GGG - chr6_4:81842224-81842243 Msa0999610:three_prime_UTR 55.0%
ACAAGGACCAAGCAGCCATG+GGG - chr6_4:81842225-81842244 Msa0999610:three_prime_UTR 55.0%
GTGTATTCCCCATGGCTGCT+TGG + chr6_4:81842235-81842254 None:intergenic 55.0%
GCAGCCATGGGGAATACACA+TGG - chr6_4:81842236-81842255 Msa0999610:three_prime_UTR 55.0%
CGCAAGCTGAAGTCTCACCT+TGG - chr6_4:81842272-81842291 Msa0999610:three_prime_UTR 55.0%
GCGTGGGAAGAACCAGCAAA+AGG + chr6_4:81842314-81842333 None:intergenic 55.0%
!! TGCTGGTTCTTCCCACGCAA+AGG - chr6_4:81842316-81842335 Msa0999610:three_prime_UTR 55.0%
! GCTGGTTCTTCCCACGCAAA+GGG - chr6_4:81842317-81842336 Msa0999610:three_prime_UTR 55.0%
CGAGGACAATTCCCTTTGCG+TGG + chr6_4:81842331-81842350 None:intergenic 55.0%
GCTGCCTTTGTTCCCAACGA+TGG - chr6_4:81842529-81842548 Msa0999610:intron 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81842162 81843090 81842162 ID=Msa0999610;Name=Msa0999610
chr6_4 mRNA 81842162 81843090 81842162 ID=Msa0999610-mRNA-1;Parent=Msa0999610;Name=Msa0999610-mRNA-1;_AED=0.02;_eAED=0.02;_QI=221|1|1|1|0.5|0.33|3|225|99
chr6_4 exon 81842162 81842527 81842162 ID=Msa0999610-mRNA-1:exon:15646;Parent=Msa0999610-mRNA-1
chr6_4 exon 81842676 81842920 81842676 ID=Msa0999610-mRNA-1:exon:15645;Parent=Msa0999610-mRNA-1
chr6_4 exon 81842956 81843090 81842956 ID=Msa0999610-mRNA-1:exon:15644;Parent=Msa0999610-mRNA-1
chr6_4 five_prime_UTR 81842956 81843090 81842956 ID=Msa0999610-mRNA-1:five_prime_utr;Parent=Msa0999610-mRNA-1
chr6_4 five_prime_UTR 81842835 81842920 81842835 ID=Msa0999610-mRNA-1:five_prime_utr;Parent=Msa0999610-mRNA-1
chr6_4 CDS 81842676 81842834 81842676 ID=Msa0999610-mRNA-1:cds;Parent=Msa0999610-mRNA-1
chr6_4 CDS 81842387 81842527 81842387 ID=Msa0999610-mRNA-1:cds;Parent=Msa0999610-mRNA-1
chr6_4 three_prime_UTR 81842162 81842386 81842162 ID=Msa0999610-mRNA-1:three_prime_utr;Parent=Msa0999610-mRNA-1
Gene Sequence

>Msa0999610

ATGCTAAGAAATGATTGTTGTTTTATTTACAAAGGTATTGAGGCTAAACAGCCCAACTCAGCTATTCGTAAGTGTGCCAGGGTACAACTAATCAAGAATGGAAAGAAAATTGCTGCCTTTGTTCCCAACGATGGTTGTTTAAACTACATCGAAGAAAATGATGAAGTTTTGATTGCTGGATTTGGAAGAAAGGGTCATGCTGTGGGAGATATTCCTGGAGTTAGATTCAAGGTTGTGAAGGTCTCTGGGGTGTCTCTTCTTGCCCTTTTCAAGGAGAAGAAGGAGAAGCCAAGATCTTAG
Protein sequence

>Msa0999610

MLRNDCCFIYKGIEAKQPNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRS*