Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367910 | RZB48484.1 | 90.850 | 153 | 4 | 1 | 8 | 160 | 96 | 238 | 2.31e-93 | 280 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367910 | sp|Q9M5Z9|RS23_EUPES | 90.789 | 152 | 4 | 1 | 9 | 160 | 1 | 142 | 5.85e-95 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367910 | A0A445FI55 | 90.850 | 153 | 4 | 1 | 8 | 160 | 96 | 238 | 1.10e-93 | 280 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0367910 | Msa0490820 | 0.803413 | 3.528306e-49 | -8.615850e-47 |
| Msa0367910 | Msa0705040 | 0.800061 | 1.719549e-48 | -8.615850e-47 |
| Msa0367910 | Msa0887590 | 0.846578 | 1.989617e-59 | -8.615850e-47 |
| Msa0367910 | Msa0999610 | 0.857759 | 1.311246e-62 | -8.615850e-47 |
| Msa0367910 | Msa1146440 | 0.968476 | 9.895427e-129 | -8.615850e-47 |
| Msa0367910 | Msa1452980 | 0.954802 | 1.299625e-112 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367910 | MtrunA17_Chr1g0181621 | 91.447 | 152 | 3 | 1 | 9 | 160 | 1 | 142 | 6.90e-97 | 276 |
| Msa0367910 | MtrunA17_Chr7g0263691 | 85.526 | 152 | 12 | 1 | 9 | 160 | 1 | 142 | 9.04e-90 | 258 |
| Msa0367910 | MtrunA17_Chr7g0239631 | 60.000 | 55 | 20 | 1 | 73 | 127 | 29 | 81 | 2.55e-15 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367910 | AT5G02960.1 | 86.842 | 152 | 10 | 1 | 9 | 160 | 1 | 142 | 6.61e-90 | 258 |
| Msa0367910 | AT3G09680.1 | 84.868 | 152 | 13 | 1 | 9 | 160 | 1 | 142 | 2.34e-86 | 249 |
Find 47 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAAGGTTGTGAAGGTCTC+TGG | 0.340450 | 3_1:+63920306 | Msa0367910:CDS |
| TCTCCTTCTTCTCCTTGAAA+AGG | 0.352760 | 3_1:-63920344 | None:intergenic |
| ATTCCACAAATCTAAGATCT+TGG | 0.379899 | 3_1:-63920369 | None:intergenic |
| CAGCTATTCGTAAGTGTGCC+AGG | 0.413818 | 3_1:+63919991 | Msa0367910:CDS |
| CATGCTGTGGGAGATATTCC+TGG | 0.414411 | 3_1:+63920276 | Msa0367910:CDS |
| CTCCTTCTTCTCCTTGAAAA+GGG | 0.418297 | 3_1:-63920343 | None:intergenic |
| ACACAAGGACCAAGCAGCCA+TGG | 0.440627 | 3_1:+63919694 | Msa0367910:CDS |
| CACTTACGAATAGCTGAGTT+GGG | 0.449683 | 3_1:-63919984 | None:intergenic |
| TCATACAAGAAGTCTCATCT+TGG | 0.473075 | 3_1:+63919788 | Msa0367910:CDS |
| TCAAGGTTGTGAAGGTCTCT+GGG | 0.478339 | 3_1:+63920307 | Msa0367910:CDS |
| TAGTTTAAACAACCATCGTT+GGG | 0.487463 | 3_1:-63920056 | None:intergenic |
| TGCTGGTTCTTCCCACGCAA+AGG | 0.487634 | 3_1:+63919832 | Msa0367910:CDS |
| CTCACCGCAGGAGGCAAAGG+TGG | 0.491520 | 3_1:+63919756 | Msa0367910:CDS |
| CATGGGGAATACACATGGAA+TGG | 0.495178 | 3_1:+63919712 | Msa0367910:CDS |
| GCTGGTTCTTCCCACGCAAA+GGG | 0.497054 | 3_1:+63919833 | Msa0367910:CDS |
| GTGTATTCCCCATGGCTGCT+TGG | 0.498685 | 3_1:-63919703 | None:intergenic |
| ATTGCTGGATTTGGAAGAAA+GGG | 0.499669 | 3_1:+63920252 | Msa0367910:CDS |
| GCGTGGGAAGAACCAGCAAA+AGG | 0.504542 | 3_1:-63919827 | None:intergenic |
| CATACAAGAAGTCTCATCTT+GGG | 0.508352 | 3_1:+63919789 | Msa0367910:CDS |
| GATTGCTGGATTTGGAAGAA+AGG | 0.508603 | 3_1:+63920251 | Msa0367910:CDS |
| TATTCCTGGAGTTAGATTCA+AGG | 0.508883 | 3_1:+63920290 | Msa0367910:CDS |
| ACACTTACGAATAGCTGAGT+TGG | 0.509353 | 3_1:-63919985 | None:intergenic |
| CACAAGGACCAAGCAGCCAT+GGG | 0.511008 | 3_1:+63919695 | Msa0367910:CDS |
| AGGGTACAACTAATCAAGAA+TGG | 0.513747 | 3_1:+63920011 | Msa0367910:CDS |
| CAGCCCACCTTTGCCTCCTG+CGG | 0.522174 | 3_1:-63919760 | None:intergenic |
| CGAGGACAATTCCCTTTGCG+TGG | 0.534957 | 3_1:-63919844 | None:intergenic |
| ATGGGGAATACACATGGAAT+GGG | 0.535864 | 3_1:+63919713 | Msa0367910:CDS |
| ACAACCATCGTTGGGAGCAA+AGG | 0.539257 | 3_1:-63920048 | None:intergenic |
| GCAAGCTGAAGTCTCACCGC+AGG | 0.557712 | 3_1:+63919744 | Msa0367910:CDS |
| CATTCCATGTGTATTCCCCA+TGG | 0.562380 | 3_1:-63919711 | None:intergenic |
| AGTCTCACCGCAGGAGGCAA+AGG | 0.562641 | 3_1:+63919753 | Msa0367910:CDS |
| GCTGCCTTTGCTCCCAACGA+TGG | 0.564470 | 3_1:+63920044 | Msa0367910:CDS |
| GTAGTTTAAACAACCATCGT+TGG | 0.564796 | 3_1:-63920057 | None:intergenic |
| AGCTGAAGTCTCACCGCAGG+AGG | 0.569090 | 3_1:+63919747 | Msa0367910:CDS |
| AGTCTCATCTTGGGAATGAG+TGG | 0.571456 | 3_1:+63919798 | Msa0367910:CDS |
| ATTCTTGATTAGTTGTACCC+TGG | 0.574592 | 3_1:-63920009 | None:intergenic |
| TGGAAGAAAGGGTCATGCTG+TGG | 0.584767 | 3_1:+63920263 | Msa0367910:CDS |
| GGAAGAAAGGGTCATGCTGT+GGG | 0.588458 | 3_1:+63920264 | Msa0367910:CDS |
| AGTTAGATTCAAGGTTGTGA+AGG | 0.593952 | 3_1:+63920299 | Msa0367910:CDS |
| TCACCGCAGGAGGCAAAGGT+GGG | 0.600190 | 3_1:+63919757 | Msa0367910:CDS |
| ACAACCTTGAATCTAACTCC+AGG | 0.602701 | 3_1:-63920294 | None:intergenic |
| ACCTCCGAGCTCAACACACA+AGG | 0.619600 | 3_1:+63919679 | None:intergenic |
| GAGGACAATTCCCTTTGCGT+GGG | 0.632164 | 3_1:-63919843 | None:intergenic |
| AGCTATTCGTAAGTGTGCCA+GGG | 0.641920 | 3_1:+63919992 | Msa0367910:CDS |
| GCAGCCATGGGGAATACACA+TGG | 0.647637 | 3_1:+63919707 | Msa0367910:CDS |
| CAAGGTTGTGAAGGTCTCTG+GGG | 0.680000 | 3_1:+63920308 | Msa0367910:CDS |
| ACAAGGACCAAGCAGCCATG+GGG | 0.741823 | 3_1:+63919696 | Msa0367910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTGTTGTTTTATTTACAA+AGG | + | chr3_1:63919945-63919964 | Msa0367910:CDS | 20.0% |
| !!! | TTTTATTTACAAAGGTATTG+AGG | + | chr3_1:63919953-63919972 | Msa0367910:CDS | 20.0% |
| !! | TGATTTTAAACTGTTATGTC+TGG | + | chr3_1:63920129-63920148 | Msa0367910:intron | 25.0% |
| TAGTTTAAACAACCATCGTT+GGG | - | chr3_1:63920059-63920078 | None:intergenic | 30.0% | |
| ! | TCCTTGCTTGTATTTTCTAT+TGG | + | chr3_1:63920097-63920116 | Msa0367910:intron | 30.0% |
| ACCAATAGAAAATACAAGCA+AGG | - | chr3_1:63920101-63920120 | None:intergenic | 30.0% | |
| TCATACAAGAAGTCTCATCT+TGG | + | chr3_1:63919788-63919807 | Msa0367910:CDS | 35.0% | |
| CATACAAGAAGTCTCATCTT+GGG | + | chr3_1:63919789-63919808 | Msa0367910:CDS | 35.0% | |
| ! | ATTCTTGATTAGTTGTACCC+TGG | - | chr3_1:63920012-63920031 | None:intergenic | 35.0% |
| AGGGTACAACTAATCAAGAA+TGG | + | chr3_1:63920011-63920030 | Msa0367910:CDS | 35.0% | |
| GTAGTTTAAACAACCATCGT+TGG | - | chr3_1:63920060-63920079 | None:intergenic | 35.0% | |
| ACACACAATGTCACACAATA+TGG | - | chr3_1:63920205-63920224 | None:intergenic | 35.0% | |
| !!! | GAAGTTTTGATTGCTGGATT+TGG | + | chr3_1:63920243-63920262 | Msa0367910:CDS | 35.0% |
| ATTGCTGGATTTGGAAGAAA+GGG | + | chr3_1:63920252-63920271 | Msa0367910:CDS | 35.0% | |
| TATTCCTGGAGTTAGATTCA+AGG | + | chr3_1:63920290-63920309 | Msa0367910:CDS | 35.0% | |
| AGTTAGATTCAAGGTTGTGA+AGG | + | chr3_1:63920299-63920318 | Msa0367910:CDS | 35.0% | |
| ATGGGGAATACACATGGAAT+GGG | + | chr3_1:63919713-63919732 | Msa0367910:CDS | 40.0% | |
| TCCTCGAAAAGATGTATGAG+TGG | + | chr3_1:63919861-63919880 | Msa0367910:intron | 40.0% | |
| ! | ACCACTCATACATCTTTTCG+AGG | - | chr3_1:63919865-63919884 | None:intergenic | 40.0% |
| GATGTATGAGTGGTTAAGCT+TGG | + | chr3_1:63919871-63919890 | Msa0367910:intron | 40.0% | |
| CACTTACGAATAGCTGAGTT+GGG | - | chr3_1:63919987-63920006 | None:intergenic | 40.0% | |
| ACACTTACGAATAGCTGAGT+TGG | - | chr3_1:63919988-63920007 | None:intergenic | 40.0% | |
| !!! | CAGGATGAAGTTTTGATTGC+TGG | + | chr3_1:63920237-63920256 | Msa0367910:intron | 40.0% |
| GATTGCTGGATTTGGAAGAA+AGG | + | chr3_1:63920251-63920270 | Msa0367910:CDS | 40.0% | |
| ACAACCTTGAATCTAACTCC+AGG | - | chr3_1:63920297-63920316 | None:intergenic | 40.0% | |
| CTCCTTCTTCTCCTTGAAAA+GGG | - | chr3_1:63920346-63920365 | None:intergenic | 40.0% | |
| TCTCCTTCTTCTCCTTGAAA+AGG | - | chr3_1:63920347-63920366 | None:intergenic | 40.0% | |
| CATTCCATGTGTATTCCCCA+TGG | - | chr3_1:63919714-63919733 | None:intergenic | 45.0% | |
| CATGGGGAATACACATGGAA+TGG | + | chr3_1:63919712-63919731 | Msa0367910:CDS | 45.0% | |
| AGTCTCATCTTGGGAATGAG+TGG | + | chr3_1:63919798-63919817 | Msa0367910:CDS | 45.0% | |
| ! | GAGTGGAAGAAACCTTTTGC+TGG | + | chr3_1:63919815-63919834 | Msa0367910:CDS | 45.0% |
| AGCTATTCGTAAGTGTGCCA+GGG | + | chr3_1:63919992-63920011 | Msa0367910:CDS | 45.0% | |
| GTGACATTGTGTGTGACTGA+TGG | + | chr3_1:63920211-63920230 | Msa0367910:intron | 45.0% | |
| TTCAAGGTTGTGAAGGTCTC+TGG | + | chr3_1:63920306-63920325 | Msa0367910:CDS | 45.0% | |
| TCAAGGTTGTGAAGGTCTCT+GGG | + | chr3_1:63920307-63920326 | Msa0367910:CDS | 45.0% | |
| ! | GTCTCTTCTTGCCCTTTTCA+AGG | + | chr3_1:63920332-63920351 | Msa0367910:CDS | 45.0% |
| !! | TGCCCTTTTCAAGGAGAAGA+AGG | + | chr3_1:63920341-63920360 | Msa0367910:CDS | 45.0% |
| GAGGACAATTCCCTTTGCGT+GGG | - | chr3_1:63919846-63919865 | None:intergenic | 50.0% | |
| CAGCTATTCGTAAGTGTGCC+AGG | + | chr3_1:63919991-63920010 | Msa0367910:CDS | 50.0% | |
| ACAACCATCGTTGGGAGCAA+AGG | - | chr3_1:63920051-63920070 | None:intergenic | 50.0% | |
| TGTGTGTGACTGATGGTTGC+AGG | + | chr3_1:63920218-63920237 | Msa0367910:intron | 50.0% | |
| TGGAAGAAAGGGTCATGCTG+TGG | + | chr3_1:63920263-63920282 | Msa0367910:CDS | 50.0% | |
| GGAAGAAAGGGTCATGCTGT+GGG | + | chr3_1:63920264-63920283 | Msa0367910:CDS | 50.0% | |
| CATGCTGTGGGAGATATTCC+TGG | + | chr3_1:63920276-63920295 | Msa0367910:CDS | 50.0% | |
| CAAGGTTGTGAAGGTCTCTG+GGG | + | chr3_1:63920308-63920327 | Msa0367910:CDS | 50.0% | |
| ACACAAGGACCAAGCAGCCA+TGG | + | chr3_1:63919694-63919713 | Msa0367910:CDS | 55.0% | |
| CACAAGGACCAAGCAGCCAT+GGG | + | chr3_1:63919695-63919714 | Msa0367910:CDS | 55.0% | |
| ACAAGGACCAAGCAGCCATG+GGG | + | chr3_1:63919696-63919715 | Msa0367910:CDS | 55.0% | |
| GTGTATTCCCCATGGCTGCT+TGG | - | chr3_1:63919706-63919725 | None:intergenic | 55.0% | |
| GCAGCCATGGGGAATACACA+TGG | + | chr3_1:63919707-63919726 | Msa0367910:CDS | 55.0% | |
| GCGTGGGAAGAACCAGCAAA+AGG | - | chr3_1:63919830-63919849 | None:intergenic | 55.0% | |
| !! | TGCTGGTTCTTCCCACGCAA+AGG | + | chr3_1:63919832-63919851 | Msa0367910:CDS | 55.0% |
| ! | GCTGGTTCTTCCCACGCAAA+GGG | + | chr3_1:63919833-63919852 | Msa0367910:CDS | 55.0% |
| CGAGGACAATTCCCTTTGCG+TGG | - | chr3_1:63919847-63919866 | None:intergenic | 55.0% | |
| GCAAGCTGAAGTCTCACCGC+AGG | + | chr3_1:63919744-63919763 | Msa0367910:CDS | 60.0% | |
| AGCTGAAGTCTCACCGCAGG+AGG | + | chr3_1:63919747-63919766 | Msa0367910:CDS | 60.0% | |
| AGTCTCACCGCAGGAGGCAA+AGG | + | chr3_1:63919753-63919772 | Msa0367910:CDS | 60.0% | |
| TCACCGCAGGAGGCAAAGGT+GGG | + | chr3_1:63919757-63919776 | Msa0367910:CDS | 60.0% | |
| GCTGCCTTTGCTCCCAACGA+TGG | + | chr3_1:63920044-63920063 | Msa0367910:CDS | 60.0% | |
| CTCACCGCAGGAGGCAAAGG+TGG | + | chr3_1:63919756-63919775 | Msa0367910:CDS | 65.0% | |
| CAGCCCACCTTTGCCTCCTG+CGG | - | chr3_1:63919763-63919782 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 63919689 | 63920380 | 63919689 | ID=Msa0367910;Name=Msa0367910 |
| chr3_1 | mRNA | 63919689 | 63920380 | 63919689 | ID=Msa0367910-mRNA-1;Parent=Msa0367910;Name=Msa0367910-mRNA-1;_AED=0.04;_eAED=0.03;_QI=0|0|0|1|0.5|0.33|3|0|160 |
| chr3_1 | exon | 63919689 | 63919879 | 63919689 | ID=Msa0367910-mRNA-1:exon:12714;Parent=Msa0367910-mRNA-1 |
| chr3_1 | exon | 63919941 | 63920091 | 63919941 | ID=Msa0367910-mRNA-1:exon:12715;Parent=Msa0367910-mRNA-1 |
| chr3_1 | exon | 63920240 | 63920380 | 63920240 | ID=Msa0367910-mRNA-1:exon:12716;Parent=Msa0367910-mRNA-1 |
| chr3_1 | CDS | 63919689 | 63919879 | 63919689 | ID=Msa0367910-mRNA-1:cds;Parent=Msa0367910-mRNA-1 |
| chr3_1 | CDS | 63919941 | 63920091 | 63919941 | ID=Msa0367910-mRNA-1:cds;Parent=Msa0367910-mRNA-1 |
| chr3_1 | CDS | 63920240 | 63920380 | 63920240 | ID=Msa0367910-mRNA-1:cds;Parent=Msa0367910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
