Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452980 | sp|Q9M5Z9|RS23_EUPES | 93.333 | 150 | 2 | 1 | 1 | 150 | 1 | 142 | 5.78e-96 | 276 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452980 | A0A445FI55 | 94.000 | 150 | 1 | 1 | 1 | 150 | 97 | 238 | 1.29e-94 | 282 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0367910 | Msa1452980 | 0.954802 | 1.299625e-112 | -8.615850e-47 |
| Msa0999610 | Msa1452980 | 0.895902 | 6.160484e-76 | -8.615850e-47 |
| Msa1146440 | Msa1452980 | 0.941108 | 7.374092e-101 | -8.615850e-47 |
| Msa0887590 | Msa1452980 | 0.895965 | 5.802055e-76 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452980 | MtrunA17_Chr1g0181621 | 94.000 | 150 | 1 | 1 | 1 | 150 | 1 | 142 | 5.23e-98 | 278 |
| Msa1452980 | MtrunA17_Chr7g0263691 | 88.000 | 150 | 10 | 1 | 1 | 150 | 1 | 142 | 1.13e-90 | 259 |
| Msa1452980 | MtrunA17_Chr7g0239631 | 60.000 | 55 | 20 | 1 | 63 | 117 | 29 | 81 | 2.52e-15 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452980 | AT5G02960.1 | 89.333 | 150 | 8 | 1 | 1 | 150 | 1 | 142 | 7.55e-91 | 260 |
| Msa1452980 | AT3G09680.1 | 87.333 | 150 | 11 | 1 | 1 | 150 | 1 | 142 | 2.29e-87 | 251 |
Find 42 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAAGGTTGTGAAGGTCTC+TGG | 0.340450 | tig0034356:-4497 | Msa1452980:CDS |
| TCTCCTTCTTCTCCTTGAAA+AGG | 0.352760 | tig0034356:+4459 | None:intergenic |
| ATTCCACAAATCTAAGATCT+TGG | 0.379899 | tig0034356:+4434 | None:intergenic |
| CAGCTATTCGTAAGTGTGCC+AGG | 0.413818 | tig0034356:-4812 | Msa1452980:CDS |
| CATGCTGTGGGAGATATTCC+TGG | 0.414411 | tig0034356:-4527 | Msa1452980:CDS |
| CTCCTTCTTCTCCTTGAAAA+GGG | 0.418297 | tig0034356:+4460 | None:intergenic |
| CACTTACGAATAGCTGAGTT+GGG | 0.449683 | tig0034356:+4819 | None:intergenic |
| TCATACAAGAAGTCTCATCT+TGG | 0.473075 | tig0034356:-5015 | Msa1452980:CDS |
| TCAAGGTTGTGAAGGTCTCT+GGG | 0.478339 | tig0034356:-4496 | Msa1452980:CDS |
| TGCTGGTTCTTCCCACGCAA+AGG | 0.487634 | tig0034356:-4971 | Msa1452980:CDS |
| CTCACCGCAGGAGGCAAAGG+TGG | 0.491520 | tig0034356:-5047 | Msa1452980:CDS |
| CATGGGGAATACACATGGAA+TGG | 0.495178 | tig0034356:-5091 | Msa1452980:CDS |
| GCTGGTTCTTCCCACGCAAA+GGG | 0.497054 | tig0034356:-4970 | Msa1452980:CDS |
| TAGTTTAAACAACCATCGTT+GGG | 0.498002 | tig0034356:+4747 | None:intergenic |
| ATTGCTGGATTTGGAAGAAA+GGG | 0.499669 | tig0034356:-4551 | Msa1452980:CDS |
| GCGTGGGAAGAACCAGCAAA+AGG | 0.504542 | tig0034356:+4976 | None:intergenic |
| CATACAAGAAGTCTCATCTT+GGG | 0.508352 | tig0034356:-5014 | Msa1452980:CDS |
| GATTGCTGGATTTGGAAGAA+AGG | 0.508603 | tig0034356:-4552 | Msa1452980:CDS |
| TATTCCTGGAGTTAGATTCA+AGG | 0.508883 | tig0034356:-4513 | Msa1452980:CDS |
| ACACTTACGAATAGCTGAGT+TGG | 0.509353 | tig0034356:+4818 | None:intergenic |
| AGGGTACAACTAATCAAGAA+TGG | 0.513747 | tig0034356:-4792 | Msa1452980:CDS |
| CAGCCCACCTTTGCCTCCTG+CGG | 0.522174 | tig0034356:+5043 | None:intergenic |
| CGAGGACAATTCCCTTTGCG+TGG | 0.534957 | tig0034356:+4959 | None:intergenic |
| ATGGGGAATACACATGGAAT+GGG | 0.535864 | tig0034356:-5090 | Msa1452980:CDS |
| GTAGTTTAAACAACCATCGT+TGG | 0.552087 | tig0034356:+4746 | None:intergenic |
| GCAAGCTGAAGTCTCACCGC+AGG | 0.557712 | tig0034356:-5059 | Msa1452980:CDS |
| CATTCCATGTGTATTCCCCA+TGG | 0.562380 | tig0034356:+5092 | None:intergenic |
| AGTCTCACCGCAGGAGGCAA+AGG | 0.562641 | tig0034356:-5050 | Msa1452980:CDS |
| AGCTGAAGTCTCACCGCAGG+AGG | 0.569090 | tig0034356:-5056 | Msa1452980:CDS |
| AGTCTCATCTTGGGAATGAG+TGG | 0.571456 | tig0034356:-5005 | Msa1452980:CDS |
| ACAACCATCGTTGGGAACAA+AGG | 0.572957 | tig0034356:+4755 | None:intergenic |
| ATTCTTGATTAGTTGTACCC+TGG | 0.574592 | tig0034356:+4794 | None:intergenic |
| TGGAAGAAAGGGTCATGCTG+TGG | 0.584767 | tig0034356:-4540 | Msa1452980:CDS |
| GGAAGAAAGGGTCATGCTGT+GGG | 0.588458 | tig0034356:-4539 | Msa1452980:CDS |
| AGTTAGATTCAAGGTTGTGA+AGG | 0.593952 | tig0034356:-4504 | Msa1452980:CDS |
| TCACCGCAGGAGGCAAAGGT+GGG | 0.600190 | tig0034356:-5046 | Msa1452980:CDS |
| ACAACCTTGAATCTAACTCC+AGG | 0.602701 | tig0034356:+4509 | None:intergenic |
| GCTGCCTTTGTTCCCAACGA+TGG | 0.607412 | tig0034356:-4759 | Msa1452980:CDS |
| GAGGACAATTCCCTTTGCGT+GGG | 0.632164 | tig0034356:+4960 | None:intergenic |
| AGCTATTCGTAAGTGTGCCA+GGG | 0.641920 | tig0034356:-4811 | Msa1452980:CDS |
| GCAGCCATGGGGAATACACA+TGG | 0.647637 | tig0034356:-5096 | None:intergenic |
| CAAGGTTGTGAAGGTCTCTG+GGG | 0.680000 | tig0034356:-4495 | Msa1452980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTGTTGTTTTATTTACAA+AGG | - | tig0034356:4677-4696 | Msa1452980:intron | 20.0% |
| !!! | TTTTATTTACAAAGGTATTG+AGG | - | tig0034356:4685-4704 | Msa1452980:intron | 20.0% |
| !! | TGATTTTAAACTGTTATGTC+TGG | - | tig0034356:4861-4880 | Msa1452980:CDS | 25.0% |
| TAGTTTAAACAACCATCGTT+GGG | + | tig0034356:4791-4810 | None:intergenic | 30.0% | |
| ! | TCCTTGCTTGTATTTTCTAT+TGG | - | tig0034356:4829-4848 | Msa1452980:CDS | 30.0% |
| ACCAATAGAAAATACAAGCA+AGG | + | tig0034356:4833-4852 | None:intergenic | 30.0% | |
| TCATACAAGAAGTCTCATCT+TGG | - | tig0034356:4520-4539 | Msa1452980:CDS | 35.0% | |
| CATACAAGAAGTCTCATCTT+GGG | - | tig0034356:4521-4540 | Msa1452980:CDS | 35.0% | |
| ! | ATTCTTGATTAGTTGTACCC+TGG | + | tig0034356:4744-4763 | None:intergenic | 35.0% |
| AGGGTACAACTAATCAAGAA+TGG | - | tig0034356:4743-4762 | Msa1452980:CDS | 35.0% | |
| GTAGTTTAAACAACCATCGT+TGG | + | tig0034356:4792-4811 | None:intergenic | 35.0% | |
| ACACACAATGTCACACAATA+TGG | + | tig0034356:4937-4956 | None:intergenic | 35.0% | |
| !!! | GAAGTTTTGATTGCTGGATT+TGG | - | tig0034356:4975-4994 | Msa1452980:CDS | 35.0% |
| ATTGCTGGATTTGGAAGAAA+GGG | - | tig0034356:4984-5003 | Msa1452980:CDS | 35.0% | |
| TATTCCTGGAGTTAGATTCA+AGG | - | tig0034356:5022-5041 | Msa1452980:CDS | 35.0% | |
| AGTTAGATTCAAGGTTGTGA+AGG | - | tig0034356:5031-5050 | Msa1452980:CDS | 35.0% | |
| ATGGGGAATACACATGGAAT+GGG | - | tig0034356:4445-4464 | Msa1452980:CDS | 40.0% | |
| TCCTCGAAAAGATGTAAGAG+TGG | - | tig0034356:4593-4612 | Msa1452980:intron | 40.0% | |
| ! | ACCACTCTTACATCTTTTCG+AGG | + | tig0034356:4597-4616 | None:intergenic | 40.0% |
| GATGTAAGAGTGGTTAAGCT+TGG | - | tig0034356:4603-4622 | Msa1452980:intron | 40.0% | |
| CACTTACGAATAGCTGAGTT+GGG | + | tig0034356:4719-4738 | None:intergenic | 40.0% | |
| ACACTTACGAATAGCTGAGT+TGG | + | tig0034356:4720-4739 | None:intergenic | 40.0% | |
| !!! | CAGGATGAAGTTTTGATTGC+TGG | - | tig0034356:4969-4988 | Msa1452980:CDS | 40.0% |
| GATTGCTGGATTTGGAAGAA+AGG | - | tig0034356:4983-5002 | Msa1452980:CDS | 40.0% | |
| ACAACCTTGAATCTAACTCC+AGG | + | tig0034356:5029-5048 | None:intergenic | 40.0% | |
| CTCCTTCTTCTCCTTGAAAA+GGG | + | tig0034356:5078-5097 | None:intergenic | 40.0% | |
| TCTCCTTCTTCTCCTTGAAA+AGG | + | tig0034356:5079-5098 | None:intergenic | 40.0% | |
| AGTCTCATCTTGGGAATGAG+TGG | - | tig0034356:4530-4549 | Msa1452980:CDS | 45.0% | |
| ! | GAGTGGAAGAAACCTTTTGC+TGG | - | tig0034356:4547-4566 | Msa1452980:CDS | 45.0% |
| AGCTATTCGTAAGTGTGCCA+GGG | - | tig0034356:4724-4743 | Msa1452980:intron | 45.0% | |
| ACAACCATCGTTGGGAACAA+AGG | + | tig0034356:4783-4802 | None:intergenic | 45.0% | |
| GTGACATTGTGTGTGACTGA+TGG | - | tig0034356:4943-4962 | Msa1452980:intron | 45.0% | |
| TTCAAGGTTGTGAAGGTCTC+TGG | - | tig0034356:5038-5057 | Msa1452980:CDS | 45.0% | |
| TCAAGGTTGTGAAGGTCTCT+GGG | - | tig0034356:5039-5058 | Msa1452980:CDS | 45.0% | |
| ! | GTCTCTTCTTGCCCTTTTCA+AGG | - | tig0034356:5064-5083 | Msa1452980:CDS | 45.0% |
| !! | TGCCCTTTTCAAGGAGAAGA+AGG | - | tig0034356:5073-5092 | Msa1452980:CDS | 45.0% |
| GAGGACAATTCCCTTTGCGT+GGG | + | tig0034356:4578-4597 | None:intergenic | 50.0% | |
| CAGCTATTCGTAAGTGTGCC+AGG | - | tig0034356:4723-4742 | Msa1452980:intron | 50.0% | |
| TGTGTGTGACTGATGGTTGC+AGG | - | tig0034356:4950-4969 | Msa1452980:intron | 50.0% | |
| TGGAAGAAAGGGTCATGCTG+TGG | - | tig0034356:4995-5014 | Msa1452980:CDS | 50.0% | |
| GGAAGAAAGGGTCATGCTGT+GGG | - | tig0034356:4996-5015 | Msa1452980:CDS | 50.0% | |
| CATGCTGTGGGAGATATTCC+TGG | - | tig0034356:5008-5027 | Msa1452980:CDS | 50.0% | |
| CAAGGTTGTGAAGGTCTCTG+GGG | - | tig0034356:5040-5059 | Msa1452980:CDS | 50.0% | |
| GCGTGGGAAGAACCAGCAAA+AGG | + | tig0034356:4562-4581 | None:intergenic | 55.0% | |
| !! | TGCTGGTTCTTCCCACGCAA+AGG | - | tig0034356:4564-4583 | Msa1452980:CDS | 55.0% |
| ! | GCTGGTTCTTCCCACGCAAA+GGG | - | tig0034356:4565-4584 | Msa1452980:CDS | 55.0% |
| CGAGGACAATTCCCTTTGCG+TGG | + | tig0034356:4579-4598 | None:intergenic | 55.0% | |
| GCTGCCTTTGTTCCCAACGA+TGG | - | tig0034356:4776-4795 | Msa1452980:CDS | 55.0% | |
| GCAAGCTGAAGTCTCACCGC+AGG | - | tig0034356:4476-4495 | Msa1452980:CDS | 60.0% | |
| AGCTGAAGTCTCACCGCAGG+AGG | - | tig0034356:4479-4498 | Msa1452980:CDS | 60.0% | |
| AGTCTCACCGCAGGAGGCAA+AGG | - | tig0034356:4485-4504 | Msa1452980:CDS | 60.0% | |
| TCACCGCAGGAGGCAAAGGT+GGG | - | tig0034356:4489-4508 | Msa1452980:CDS | 60.0% | |
| CTCACCGCAGGAGGCAAAGG+TGG | - | tig0034356:4488-4507 | Msa1452980:CDS | 65.0% | |
| CAGCCCACCTTTGCCTCCTG+CGG | + | tig0034356:4495-4514 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0034356 | gene | 4445 | 5112 | 4445 | ID=Msa1452980;Name=Msa1452980 |
| tig0034356 | mRNA | 4445 | 5112 | 4445 | ID=Msa1452980-mRNA-1;Parent=Msa1452980;Name=Msa1452980-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|0.5|0.33|3|0|150 |
| tig0034356 | exon | 4952 | 5112 | 4952 | ID=Msa1452980-mRNA-1:exon:18956;Parent=Msa1452980-mRNA-1 |
| tig0034356 | exon | 4734 | 4884 | 4734 | ID=Msa1452980-mRNA-1:exon:18955;Parent=Msa1452980-mRNA-1 |
| tig0034356 | exon | 4445 | 4585 | 4445 | ID=Msa1452980-mRNA-1:exon:18954;Parent=Msa1452980-mRNA-1 |
| tig0034356 | CDS | 4952 | 5112 | 4952 | ID=Msa1452980-mRNA-1:cds;Parent=Msa1452980-mRNA-1 |
| tig0034356 | CDS | 4734 | 4884 | 4734 | ID=Msa1452980-mRNA-1:cds;Parent=Msa1452980-mRNA-1 |
| tig0034356 | CDS | 4445 | 4585 | 4445 | ID=Msa1452980-mRNA-1:cds;Parent=Msa1452980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
