Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1013360 | XP_039683558.1 | 80.328 | 61 | 12 | 0 | 17 | 77 | 132 | 192 | 1.11e-22 | 96.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1013360 | A0A396H1H4 | 80.328 | 61 | 12 | 0 | 17 | 77 | 132 | 192 | 5.32e-23 | 96.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1013360 | Msa1099960 | 0.801401 | 9.162512e-49 | -8.615850e-47 |
| Msa1013360 | Msa1350680 | 0.800107 | 1.683045e-48 | -8.615850e-47 |
| Msa1013360 | Msa1388160 | 0.834742 | 2.513570e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1013360 | MtrunA17_Chr7g0225251 | 80.328 | 61 | 12 | 0 | 17 | 77 | 132 | 192 | 1.03e-26 | 96.3 |
| Msa1013360 | MtrunA17_Chr7g0225371 | 80.328 | 61 | 12 | 0 | 17 | 77 | 193 | 253 | 3.52e-26 | 96.3 |
| Msa1013360 | MtrunA17_Chr7g0225341 | 83.019 | 53 | 8 | 1 | 17 | 69 | 66 | 117 | 1.10e-23 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCTTCGTCTATTAGCTT+TGG | 0.380513 | 7_1:+19836586 | Msa1013360:CDS |
| TGCTAAAACAAGGTCTTCTA+TGG | 0.383482 | 7_1:-19836536 | None:intergenic |
| ATGACATGCCTTTGGTGTTA+CGG | 0.399250 | 7_1:-19836612 | None:intergenic |
| GATGCTACTGCCTACTTCTG+TGG | 0.469527 | 7_1:-19836371 | None:intergenic |
| AGGGACAAATGACATGCCTT+TGG | 0.478422 | 7_1:-19836620 | None:intergenic |
| CGAGCTGTTCAGTGTGGAAA+GGG | 0.479849 | 7_1:+19836643 | Msa1013360:CDS |
| ATGCTACTGCCTACTTCTGT+GGG | 0.480454 | 7_1:-19836370 | None:intergenic |
| GTGTGGAAAGGGATCAAACT+CGG | 0.545538 | 7_1:+19836654 | Msa1013360:CDS |
| TCGAGCTGTTCAGTGTGGAA+AGG | 0.580780 | 7_1:+19836642 | Msa1013360:CDS |
| AGTCTCTAGAGTGAATACTT+TGG | 0.586283 | 7_1:+19836558 | Msa1013360:CDS |
| TTTGGTTGCCGTAACACCAA+AGG | 0.586816 | 7_1:+19836604 | Msa1013360:CDS |
| CTCTAGAGACTGCTAAAACA+AGG | 0.590843 | 7_1:-19836546 | None:intergenic |
| TGCTTCAGGAATGAATGAAG+TGG | 0.597871 | 7_1:+19836504 | Msa1013360:intron |
| TTTCCACACTGAACAGCTCG+AGG | 0.606690 | 7_1:-19836640 | None:intergenic |
| TGTGGAAAGGGATCAAACTC+GGG | 0.608253 | 7_1:+19836655 | Msa1013360:CDS |
| ATGATGCTTAGTGAAGGTTG+CGG | 0.638949 | 7_1:+19836333 | Msa1013360:CDS |
| TTCCACACTGAACAGCTCGA+GGG | 0.645561 | 7_1:-19836639 | None:intergenic |
| GTCCCTCGAGCTGTTCAGTG+TGG | 0.654976 | 7_1:+19836637 | Msa1013360:CDS |
| TATTGTATGATGCTTAGTGA+AGG | 0.677939 | 7_1:+19836327 | None:intergenic |
| CAACCAAAGCTAATAGACGA+AGG | 0.701927 | 7_1:-19836589 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAAAAAACTCGAATGATGT+CGG | - | chr7_1:19836417-19836436 | None:intergenic | 30.0% | |
| CAAAAAACATTCTCATAAGG+AGG | + | chr7_1:19836480-19836499 | Msa1013360:intron | 30.0% | |
| AATTCAAAACCCACAGAAGT+AGG | + | chr7_1:19836361-19836380 | Msa1013360:intron | 35.0% | |
| GAAAAAACTCGAATGATGTC+GGG | - | chr7_1:19836416-19836435 | None:intergenic | 35.0% | |
| GCGCAAAAAACATTCTCATA+AGG | + | chr7_1:19836477-19836496 | Msa1013360:intron | 35.0% | |
| TGCTAAAACAAGGTCTTCTA+TGG | - | chr7_1:19836539-19836558 | None:intergenic | 35.0% | |
| !! | AGTCTCTAGAGTGAATACTT+TGG | + | chr7_1:19836558-19836577 | Msa1013360:CDS | 35.0% |
| ATGATGCTTAGTGAAGGTTG+CGG | + | chr7_1:19836333-19836352 | Msa1013360:CDS | 40.0% | |
| CTCTGCTTTCAAAGAAACTG+AGG | + | chr7_1:19836443-19836462 | Msa1013360:intron | 40.0% | |
| TGCTTCAGGAATGAATGAAG+TGG | + | chr7_1:19836504-19836523 | Msa1013360:intron | 40.0% | |
| ! | CTCTAGAGACTGCTAAAACA+AGG | - | chr7_1:19836549-19836568 | None:intergenic | 40.0% |
| ! | ACTCCTTCGTCTATTAGCTT+TGG | + | chr7_1:19836586-19836605 | Msa1013360:CDS | 40.0% |
| CAACCAAAGCTAATAGACGA+AGG | - | chr7_1:19836592-19836611 | None:intergenic | 40.0% | |
| !! | ATGACATGCCTTTGGTGTTA+CGG | - | chr7_1:19836615-19836634 | None:intergenic | 40.0% |
| ATGCTACTGCCTACTTCTGT+GGG | - | chr7_1:19836373-19836392 | None:intergenic | 45.0% | |
| TCTCATAAGGAGGATGCTTC+AGG | + | chr7_1:19836490-19836509 | Msa1013360:intron | 45.0% | |
| TTTGGTTGCCGTAACACCAA+AGG | + | chr7_1:19836604-19836623 | Msa1013360:CDS | 45.0% | |
| AGGGACAAATGACATGCCTT+TGG | - | chr7_1:19836623-19836642 | None:intergenic | 45.0% | |
| GTGTGGAAAGGGATCAAACT+CGG | + | chr7_1:19836654-19836673 | Msa1013360:CDS | 45.0% | |
| TGTGGAAAGGGATCAAACTC+GGG | + | chr7_1:19836655-19836674 | Msa1013360:CDS | 45.0% | |
| GATGCTACTGCCTACTTCTG+TGG | - | chr7_1:19836374-19836393 | None:intergenic | 50.0% | |
| CAGAAGTAGGCAGTAGCATC+TGG | + | chr7_1:19836374-19836393 | Msa1013360:intron | 50.0% | |
| TTCCACACTGAACAGCTCGA+GGG | - | chr7_1:19836642-19836661 | None:intergenic | 50.0% | |
| TTTCCACACTGAACAGCTCG+AGG | - | chr7_1:19836643-19836662 | None:intergenic | 50.0% | |
| TCGAGCTGTTCAGTGTGGAA+AGG | + | chr7_1:19836642-19836661 | Msa1013360:CDS | 50.0% | |
| CGAGCTGTTCAGTGTGGAAA+GGG | + | chr7_1:19836643-19836662 | Msa1013360:CDS | 50.0% | |
| GTCCCTCGAGCTGTTCAGTG+TGG | + | chr7_1:19836637-19836656 | Msa1013360:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 19836333 | 19836702 | 19836333 | ID=Msa1013360;Name=Msa1013360 |
| chr7_1 | mRNA | 19836333 | 19836702 | 19836333 | ID=Msa1013360-mRNA-1;Parent=Msa1013360;Name=Msa1013360-mRNA-1;_AED=0.31;_eAED=0.33;_QI=0|0|0|1|0|0|2|0|78 |
| chr7_1 | exon | 19836333 | 19836378 | 19836333 | ID=Msa1013360-mRNA-1:exon:4812;Parent=Msa1013360-mRNA-1 |
| chr7_1 | exon | 19836512 | 19836702 | 19836512 | ID=Msa1013360-mRNA-1:exon:4813;Parent=Msa1013360-mRNA-1 |
| chr7_1 | CDS | 19836333 | 19836378 | 19836333 | ID=Msa1013360-mRNA-1:cds;Parent=Msa1013360-mRNA-1 |
| chr7_1 | CDS | 19836512 | 19836702 | 19836512 | ID=Msa1013360-mRNA-1:cds;Parent=Msa1013360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
