Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1026230 | Msa1064990 | 0.969473 | 3.563016e-130 | -8.615850e-47 |
| Msa1026230 | Msa1153750 | 0.938837 | 3.472257e-99 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTCATCAAGTATTAAAA+AGG | 0.314463 | 7_1:-57873447 | None:intergenic |
| CCGAAATTTCCAGCTACTTC+AGG | 0.353667 | 7_1:-57873515 | None:intergenic |
| CCTGAAGTAGCTGGAAATTT+CGG | 0.364353 | 7_1:+57873515 | Msa1026230:CDS |
| TTCCTTCTCTGAGGTTAATG+AGG | 0.457881 | 7_1:+57873403 | Msa1026230:CDS |
| AAACCCGATGTTTGTGAAGA+TGG | 0.464513 | 7_1:+57873554 | Msa1026230:CDS |
| ACTCATGTGCCTGAAGTAGC+TGG | 0.502856 | 7_1:+57873506 | Msa1026230:CDS |
| CAACCATCTTCACAAACATC+GGG | 0.513442 | 7_1:-57873557 | None:intergenic |
| TCAACCATCTTCACAAACAT+CGG | 0.556064 | 7_1:-57873558 | None:intergenic |
| CATGAGTACAAAATAGATGT+TGG | 0.570802 | 7_1:-57873490 | None:intergenic |
| AACCTCATTAACCTCAGAGA+AGG | 0.624569 | 7_1:-57873405 | None:intergenic |
| TACAAAATAGATGTTGGTGG+TGG | 0.639103 | 7_1:-57873484 | None:intergenic |
| AGTCAAGAGTTCCTTCTCTG+AGG | 0.653235 | 7_1:+57873394 | Msa1026230:CDS |
| GAGTACAAAATAGATGTTGG+TGG | 0.682447 | 7_1:-57873487 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTCATCAAGTATTAAAA+AGG | - | chr7_1:57873450-57873469 | None:intergenic | 20.0% |
| !!! | TTTTTAATACTTGATGAGAT+TGG | + | chr7_1:57873449-57873468 | Msa1026230:CDS | 20.0% |
| !! | ATTAAAAAGGTTAGTTCAGA+TGG | - | chr7_1:57873437-57873456 | None:intergenic | 25.0% |
| CATGAGTACAAAATAGATGT+TGG | - | chr7_1:57873493-57873512 | None:intergenic | 30.0% | |
| ! | TACAAAATAGATGTTGGTGG+TGG | - | chr7_1:57873487-57873506 | None:intergenic | 35.0% |
| GAGTACAAAATAGATGTTGG+TGG | - | chr7_1:57873490-57873509 | None:intergenic | 35.0% | |
| TCAACCATCTTCACAAACAT+CGG | - | chr7_1:57873561-57873580 | None:intergenic | 35.0% | |
| !! | TTCCTTCTCTGAGGTTAATG+AGG | + | chr7_1:57873403-57873422 | Msa1026230:CDS | 40.0% |
| AACCTCATTAACCTCAGAGA+AGG | - | chr7_1:57873408-57873427 | None:intergenic | 40.0% | |
| CCTGAAGTAGCTGGAAATTT+CGG | + | chr7_1:57873515-57873534 | Msa1026230:CDS | 40.0% | |
| AAACCCGATGTTTGTGAAGA+TGG | + | chr7_1:57873554-57873573 | Msa1026230:CDS | 40.0% | |
| CAACCATCTTCACAAACATC+GGG | - | chr7_1:57873560-57873579 | None:intergenic | 40.0% | |
| AGTCAAGAGTTCCTTCTCTG+AGG | + | chr7_1:57873394-57873413 | Msa1026230:CDS | 45.0% | |
| CCGAAATTTCCAGCTACTTC+AGG | - | chr7_1:57873518-57873537 | None:intergenic | 45.0% | |
| ACTCATGTGCCTGAAGTAGC+TGG | + | chr7_1:57873506-57873525 | Msa1026230:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 57873383 | 57873580 | 57873383 | ID=Msa1026230;Name=Msa1026230 |
| chr7_1 | mRNA | 57873383 | 57873580 | 57873383 | ID=Msa1026230-mRNA-1;Parent=Msa1026230;Name=Msa1026230-mRNA-1;_AED=0.44;_eAED=1.00;_QI=0|-1|0|1|-1|1|1|0|65 |
| chr7_1 | exon | 57873383 | 57873580 | 57873383 | ID=Msa1026230-mRNA-1:exon:11574;Parent=Msa1026230-mRNA-1 |
| chr7_1 | CDS | 57873383 | 57873580 | 57873383 | ID=Msa1026230-mRNA-1:cds;Parent=Msa1026230-mRNA-1 |
| Gene Sequence |
| Protein sequence |
