Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0372290 | Msa1064990 | 0.802107 | 6.564435e-49 | -8.615850e-47 |
| Msa0466780 | Msa1064990 | 0.809950 | 1.468266e-50 | -8.615850e-47 |
| Msa1026230 | Msa1064990 | 0.969473 | 3.563016e-130 | -8.615850e-47 |
| Msa1064990 | Msa1153750 | 0.940735 | 1.402362e-100 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTCATCAAGTATTAAAA+AGG | 0.314463 | 7_2:-58402054 | None:intergenic |
| CCGAAATTTCCAGCTACTTC+AGG | 0.353667 | 7_2:-58402122 | None:intergenic |
| CCTGAAGTAGCTGGAAATTT+CGG | 0.364353 | 7_2:+58402122 | Msa1064990:CDS |
| TTCCTTCTCTGAGGTTAATG+AGG | 0.457881 | 7_2:+58402010 | Msa1064990:CDS |
| AAACCCGATGTTTGTGAAGA+TGG | 0.464513 | 7_2:+58402161 | Msa1064990:CDS |
| ACTCATGTGCCTGAAGTAGC+TGG | 0.502856 | 7_2:+58402113 | Msa1064990:CDS |
| CAACCATCTTCACAAACATC+GGG | 0.513442 | 7_2:-58402164 | None:intergenic |
| TCAACCATCTTCACAAACAT+CGG | 0.556064 | 7_2:-58402165 | None:intergenic |
| CATGAGTACAAAATAGATGT+TGG | 0.570802 | 7_2:-58402097 | None:intergenic |
| AACCTCATTAACCTCAGAGA+AGG | 0.624569 | 7_2:-58402012 | None:intergenic |
| TACAAAATAGATGTTGGTGG+TGG | 0.639103 | 7_2:-58402091 | None:intergenic |
| AGTCAAGAGTTCCTTCTCTG+AGG | 0.653235 | 7_2:+58402001 | Msa1064990:CDS |
| GAGTACAAAATAGATGTTGG+TGG | 0.682447 | 7_2:-58402094 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTCATCAAGTATTAAAA+AGG | - | chr7_2:58402057-58402076 | None:intergenic | 20.0% |
| !!! | TTTTTAATACTTGATGAGAT+TGG | + | chr7_2:58402056-58402075 | Msa1064990:CDS | 20.0% |
| !! | ATTAAAAAGGTTAGTTCAGA+TGG | - | chr7_2:58402044-58402063 | None:intergenic | 25.0% |
| CATGAGTACAAAATAGATGT+TGG | - | chr7_2:58402100-58402119 | None:intergenic | 30.0% | |
| ! | TACAAAATAGATGTTGGTGG+TGG | - | chr7_2:58402094-58402113 | None:intergenic | 35.0% |
| GAGTACAAAATAGATGTTGG+TGG | - | chr7_2:58402097-58402116 | None:intergenic | 35.0% | |
| TCAACCATCTTCACAAACAT+CGG | - | chr7_2:58402168-58402187 | None:intergenic | 35.0% | |
| !! | TTCCTTCTCTGAGGTTAATG+AGG | + | chr7_2:58402010-58402029 | Msa1064990:CDS | 40.0% |
| AACCTCATTAACCTCAGAGA+AGG | - | chr7_2:58402015-58402034 | None:intergenic | 40.0% | |
| CCTGAAGTAGCTGGAAATTT+CGG | + | chr7_2:58402122-58402141 | Msa1064990:CDS | 40.0% | |
| AAACCCGATGTTTGTGAAGA+TGG | + | chr7_2:58402161-58402180 | Msa1064990:CDS | 40.0% | |
| CAACCATCTTCACAAACATC+GGG | - | chr7_2:58402167-58402186 | None:intergenic | 40.0% | |
| AGTCAAGAGTTCCTTCTCTG+AGG | + | chr7_2:58402001-58402020 | Msa1064990:CDS | 45.0% | |
| CCGAAATTTCCAGCTACTTC+AGG | - | chr7_2:58402125-58402144 | None:intergenic | 45.0% | |
| ACTCATGTGCCTGAAGTAGC+TGG | + | chr7_2:58402113-58402132 | Msa1064990:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 58401990 | 58402187 | 58401990 | ID=Msa1064990;Name=Msa1064990 |
| chr7_2 | mRNA | 58401990 | 58402187 | 58401990 | ID=Msa1064990-mRNA-1;Parent=Msa1064990;Name=Msa1064990-mRNA-1;_AED=0.46;_eAED=1.00;_QI=0|-1|0|1|-1|1|1|0|65 |
| chr7_2 | exon | 58401990 | 58402187 | 58401990 | ID=Msa1064990-mRNA-1:exon:11079;Parent=Msa1064990-mRNA-1 |
| chr7_2 | CDS | 58401990 | 58402187 | 58401990 | ID=Msa1064990-mRNA-1:cds;Parent=Msa1064990-mRNA-1 |
| Gene Sequence |
| Protein sequence |
