Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1026230 | Msa1153750 | 0.938837 | 3.472257e-99 | -8.615850e-47 |
| Msa1064990 | Msa1153750 | 0.940735 | 1.402362e-100 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTCATCAAGTATTAAAA+AGG | 0.314463 | 7_4:-40777770 | None:intergenic |
| CCGAAATTTCCAGCTACTTC+AGG | 0.353667 | 7_4:-40777838 | None:intergenic |
| CCTGAAGTAGCTGGAAATTT+CGG | 0.364353 | 7_4:+40777838 | Msa1153750:CDS |
| TTCCTTCTCTGAGGTTAATG+AGG | 0.457881 | 7_4:+40777726 | Msa1153750:CDS |
| AAACCCGATGTTTGTGAAGA+TGG | 0.464513 | 7_4:+40777877 | Msa1153750:CDS |
| ACTCATGTGCCTGAAGTAGC+TGG | 0.502856 | 7_4:+40777829 | Msa1153750:CDS |
| CAACCATCTTCACAAACATC+GGG | 0.513442 | 7_4:-40777880 | None:intergenic |
| TCAACCATCTTCACAAACAT+CGG | 0.556064 | 7_4:-40777881 | None:intergenic |
| CATGAGTACAAAATAGATGT+TGG | 0.570802 | 7_4:-40777813 | None:intergenic |
| AACCTCATTAACCTCAGAGA+AGG | 0.624569 | 7_4:-40777728 | None:intergenic |
| TACAAAATAGATGTTGGTGG+TGG | 0.639103 | 7_4:-40777807 | None:intergenic |
| AGTCAAGAGTTCCTTCTCTG+AGG | 0.653235 | 7_4:+40777717 | Msa1153750:CDS |
| GAGTACAAAATAGATGTTGG+TGG | 0.682447 | 7_4:-40777810 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTCATCAAGTATTAAAA+AGG | - | chr7_4:40777773-40777792 | None:intergenic | 20.0% |
| !!! | TTTTTAATACTTGATGAGAT+TGG | + | chr7_4:40777772-40777791 | Msa1153750:CDS | 20.0% |
| !! | ATTAAAAAGGTTAGTTCAGA+TGG | - | chr7_4:40777760-40777779 | None:intergenic | 25.0% |
| CATGAGTACAAAATAGATGT+TGG | - | chr7_4:40777816-40777835 | None:intergenic | 30.0% | |
| ! | TACAAAATAGATGTTGGTGG+TGG | - | chr7_4:40777810-40777829 | None:intergenic | 35.0% |
| GAGTACAAAATAGATGTTGG+TGG | - | chr7_4:40777813-40777832 | None:intergenic | 35.0% | |
| TCAACCATCTTCACAAACAT+CGG | - | chr7_4:40777884-40777903 | None:intergenic | 35.0% | |
| !! | TTCCTTCTCTGAGGTTAATG+AGG | + | chr7_4:40777726-40777745 | Msa1153750:CDS | 40.0% |
| AACCTCATTAACCTCAGAGA+AGG | - | chr7_4:40777731-40777750 | None:intergenic | 40.0% | |
| CCTGAAGTAGCTGGAAATTT+CGG | + | chr7_4:40777838-40777857 | Msa1153750:CDS | 40.0% | |
| AAACCCGATGTTTGTGAAGA+TGG | + | chr7_4:40777877-40777896 | Msa1153750:CDS | 40.0% | |
| CAACCATCTTCACAAACATC+GGG | - | chr7_4:40777883-40777902 | None:intergenic | 40.0% | |
| AGTCAAGAGTTCCTTCTCTG+AGG | + | chr7_4:40777717-40777736 | Msa1153750:CDS | 45.0% | |
| CCGAAATTTCCAGCTACTTC+AGG | - | chr7_4:40777841-40777860 | None:intergenic | 45.0% | |
| ACTCATGTGCCTGAAGTAGC+TGG | + | chr7_4:40777829-40777848 | Msa1153750:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 40777706 | 40777903 | 40777706 | ID=Msa1153750;Name=Msa1153750 |
| chr7_4 | mRNA | 40777706 | 40777903 | 40777706 | ID=Msa1153750-mRNA-1;Parent=Msa1153750;Name=Msa1153750-mRNA-1;_AED=0.46;_eAED=1.00;_QI=0|-1|0|1|-1|1|1|0|65 |
| chr7_4 | exon | 40777706 | 40777903 | 40777706 | ID=Msa1153750-mRNA-1:exon:9887;Parent=Msa1153750-mRNA-1 |
| chr7_4 | CDS | 40777706 | 40777903 | 40777706 | ID=Msa1153750-mRNA-1:cds;Parent=Msa1153750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
