AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1030160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1030160 MtrunA17_Chr4g0044131 85.441 261 37 1 27 287 1 260 1.59e-162 453
Msa1030160 MtrunA17_Chr7g0267001 77.395 261 40 2 27 287 1 242 9.59e-141 397
Msa1030160 MtrunA17_Chr7g0267011 96.891 193 5 1 27 219 1 192 6.31e-134 378
Msa1030160 MtrunA17_Chr7g0266981 53.962 265 122 0 24 288 2 266 5.81e-96 284
Msa1030160 MtrunA17_Chr4g0044111 53.962 265 122 0 24 288 2 266 1.07e-95 284
Msa1030160 MtrunA17_Chr4g0026001 55.859 256 110 1 33 288 12 264 3.24e-94 280
Msa1030160 MtrunA17_Chr4g0025971 55.469 256 111 1 33 288 12 264 3.64e-93 277
Msa1030160 MtrunA17_Chr7g0266891 54.724 254 115 0 35 288 7 260 7.22e-93 276
Msa1030160 MtrunA17_Chr7g0244061 51.292 271 112 3 33 284 27 296 9.83e-90 270
Msa1030160 MtrunA17_Chr4g0025981 51.311 267 120 2 36 302 42 298 7.20e-88 266
Msa1030160 MtrunA17_Chr7g0268591 47.232 271 136 3 27 291 25 294 2.71e-79 243
Msa1030160 MtrunA17_Chr7g0270441 48.031 254 131 1 33 285 4 257 1.47e-75 232
Msa1030160 MtrunA17_Chr7g0252031 44.840 281 145 5 20 291 18 297 1.16e-71 223
Msa1030160 MtrunA17_Chr6g0459261 42.231 251 143 2 34 282 24 274 2.03e-71 222
Msa1030160 MtrunA17_Chr2g0324021 45.560 259 139 2 33 290 4 261 1.04e-68 214
Msa1030160 MtrunA17_Chr3g0084871 45.318 267 139 4 29 288 10 276 2.40e-68 214
Msa1030160 MtrunA17_Chr4g0040371 43.609 266 137 5 33 288 17 279 1.17e-66 210
Msa1030160 MtrunA17_Chr7g0266991 48.291 234 104 2 29 262 19 235 1.46e-66 208
Msa1030160 MtrunA17_Chr6g0459281 42.460 252 143 2 32 281 10 261 3.89e-65 206
Msa1030160 MtrunA17_Chr7g0270451 44.622 251 137 2 33 282 4 253 7.59e-65 205
Msa1030160 MtrunA17_Chr6g0459331 42.629 251 142 2 34 282 12 262 9.44e-65 204
Msa1030160 MtrunA17_Chr6g0459301 40.239 251 148 2 34 282 13 263 3.16e-64 203
Msa1030160 MtrunA17_Chr6g0459351 41.600 250 144 2 34 281 12 261 1.01e-63 202
Msa1030160 MtrunA17_Chr6g0459271 40.239 251 148 2 34 282 12 262 2.39e-62 198
Msa1030160 MtrunA17_Chr4g0044121 48.555 173 89 0 116 288 1 173 8.66e-51 166
Msa1030160 MtrunA17_Chr7g0270471 46.853 143 75 1 144 285 3 145 2.61e-37 130
Msa1030160 MtrunA17_Chr6g0463951 41.520 171 98 2 116 285 1 170 4.16e-37 130
Msa1030160 MtrunA17_Chr3g0142651 45.528 123 60 1 116 238 1 116 5.09e-28 105
Msa1030160 MtrunA17_Chr7g0270461 54.167 96 44 0 27 122 38 133 1.07e-27 105
Msa1030160 MtrunA17_Chr3g0122901 29.020 255 164 8 33 280 73 317 4.82e-26 105
Msa1030160 MtrunA17_Chr7g0272961 29.070 258 164 8 34 280 39 288 1.63e-25 103
Msa1030160 MtrunA17_Chr7g0259521 29.924 264 166 5 27 281 1 254 1.40e-24 100
Msa1030160 MtrunA17_Chr8g0357481 31.599 269 159 8 35 288 14 272 4.28e-24 99.4
Msa1030160 MtrunA17_Chr4g0075531 28.958 259 167 7 31 283 3 250 1.11e-23 97.4
Msa1030160 MtrunA17_Chr4g0028921 30.827 266 173 7 35 292 11 273 1.66e-23 97.4
Msa1030160 MtrunA17_Chr1g0191751 29.845 258 162 7 34 280 100 349 4.31e-23 97.8
Msa1030160 MtrunA17_Chr7g0259531 28.185 259 176 4 35 287 11 265 6.69e-23 95.5
Msa1030160 MtrunA17_Chr4g0028911 30.075 266 175 7 35 292 11 273 1.21e-22 95.1
Msa1030160 MtrunA17_Chr3g0112141 28.685 251 171 5 35 282 17 262 4.62e-22 93.6
Msa1030160 MtrunA17_Chr3g0112151 27.547 265 182 6 28 287 77 336 1.09e-21 93.6
Msa1030160 MtrunA17_Chr1g0182511 30.483 269 146 11 35 281 14 263 1.20e-21 92.0
Msa1030160 MtrunA17_Chr3g0112271 29.528 254 161 9 35 280 17 260 1.46e-21 92.0
Msa1030160 MtrunA17_Chr4g0074511 31.225 253 153 10 34 273 39 283 2.11e-21 92.0
Msa1030160 MtrunA17_Chr7g0239481 28.854 253 170 6 35 281 15 263 4.98e-21 90.5
Msa1030160 MtrunA17_Chr3g0112171 27.381 252 173 7 35 282 15 260 1.15e-20 89.7
Msa1030160 MtrunA17_Chr3g0112261 28.016 257 173 8 32 282 14 264 7.70e-20 87.4
Msa1030160 MtrunA17_Chr0c30g0494101 30.159 252 155 9 34 273 39 281 4.72e-19 85.5
Msa1030160 MtrunA17_Chr1g0171831 28.175 252 163 8 33 270 12 259 8.21e-19 84.3
Msa1030160 MtrunA17_Chr7g0239501 27.273 253 176 6 35 282 16 265 2.92e-18 82.8
Msa1030160 MtrunA17_Chr7g0239451 26.493 268 183 7 25 283 4 266 5.73e-18 82.0
Msa1030160 MtrunA17_Chr4g0032141 27.027 259 179 7 35 289 56 308 7.23e-18 82.4
Msa1030160 MtrunA17_Chr7g0239511 26.378 254 177 6 35 282 16 265 7.65e-18 81.6
Msa1030160 MtrunA17_Chr4g0054341 27.451 204 137 4 36 238 3 196 1.01e-16 78.6
Msa1030160 MtrunA17_Chr7g0239491 26.378 254 178 6 35 283 15 264 1.27e-16 78.2
Msa1030160 MtrunA17_Chr8g0386201 27.957 186 128 4 37 219 47 229 1.30e-15 76.3
Msa1030160 MtrunA17_Chr7g0249571 30.928 194 125 4 35 221 38 229 1.86e-15 75.5
Msa1030160 MtrunA17_Chr4g0032181 25.888 197 132 5 91 282 59 246 1.35e-14 72.4
Msa1030160 MtrunA17_Chr8g0386171 26.786 224 122 5 35 221 52 270 1.85e-14 73.2
Msa1030160 MtrunA17_Chr8g0355281 26.961 204 140 3 41 235 75 278 1.45e-13 70.5
Msa1030160 MtrunA17_Chr3g0124471 29.189 185 121 5 38 219 29 206 2.92e-13 68.9
Msa1030160 MtrunA17_Chr6g0485711 24.889 225 143 5 41 242 184 405 5.95e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1030160 AT3G51680.1 50.562 267 116 4 33 283 30 296 5.18e-78 240
Msa1030160 AT3G29250.2 46.538 260 135 4 32 289 3 260 1.47e-72 224
Msa1030160 AT4G03140.1 47.656 256 128 2 33 283 76 330 2.22e-72 227
Msa1030160 AT1G52340.1 45.113 266 136 3 33 288 16 281 2.38e-72 225
Msa1030160 AT3G29250.3 46.538 260 135 4 32 289 39 296 4.62e-72 224
Msa1030160 AT3G26770.1 45.312 256 133 3 33 282 39 293 6.83e-71 222
Msa1030160 AT2G47140.1 44.444 252 138 2 32 282 3 253 8.50e-71 220
Msa1030160 AT2G47130.1 46.245 253 131 4 32 282 3 252 1.06e-70 220
Msa1030160 AT3G29250.1 46.304 257 134 4 35 289 44 298 1.55e-70 221
Msa1030160 AT2G47120.1 42.802 257 143 4 32 287 3 256 1.42e-68 214
Msa1030160 AT2G47120.2 42.745 255 142 4 34 287 6 257 3.51e-68 213
Msa1030160 AT3G29260.1 43.191 257 143 3 32 287 3 257 9.31e-68 212
Msa1030160 AT3G26760.1 42.537 268 145 3 23 282 24 290 3.16e-66 210
Msa1030160 AT3G42960.1 42.910 268 137 8 33 288 6 269 9.59e-56 182
Msa1030160 AT5G18210.1 33.333 261 151 8 35 281 8 259 5.05e-26 104
Msa1030160 AT3G03980.1 31.538 260 161 4 35 281 14 269 8.33e-26 103
Msa1030160 AT3G05260.1 28.720 289 186 8 3 280 5 284 8.09e-25 101
Msa1030160 AT1G54870.1 30.165 242 155 5 1 232 48 285 1.07e-24 102
Msa1030160 AT1G54870.2 30.165 242 155 5 1 232 50 287 1.21e-24 102
Msa1030160 AT2G47150.1 29.435 248 120 6 35 281 1 194 2.40e-24 98.2
Msa1030160 AT3G04000.1 27.757 263 166 6 35 280 14 269 2.05e-23 97.4
Msa1030160 AT5G18210.2 33.878 245 139 8 35 265 8 243 2.21e-22 94.7
Msa1030160 AT3G12800.1 31.939 263 151 10 35 280 10 261 1.38e-21 92.8
Msa1030160 AT1G63380.4 27.985 268 179 7 35 292 19 282 1.43e-21 92.8
Msa1030160 AT1G63380.1 27.985 268 179 7 35 292 17 280 1.53e-21 92.4
Msa1030160 AT1G63380.3 27.985 268 179 7 35 292 15 278 1.79e-21 92.4
Msa1030160 AT1G63380.2 27.985 268 179 7 35 292 22 285 1.93e-21 92.4
Msa1030160 AT5G06060.1 27.519 258 177 6 35 288 9 260 2.67e-21 91.7
Msa1030160 AT2G17845.1 27.372 274 175 9 35 292 47 312 4.05e-21 92.0
Msa1030160 AT2G29340.2 27.099 262 173 7 35 288 7 258 8.58e-21 90.1
Msa1030160 AT1G62610.1 27.715 267 181 7 35 292 14 277 1.18e-20 90.1
Msa1030160 AT1G62610.3 27.715 267 181 7 35 292 15 278 1.31e-20 89.7
Msa1030160 AT1G62610.2 27.715 267 181 7 35 292 13 276 1.39e-20 89.7
Msa1030160 AT1G62610.4 27.612 268 180 7 35 292 19 282 1.49e-20 89.7
Msa1030160 AT2G29340.1 27.099 262 173 7 35 288 7 258 1.83e-20 90.1
Msa1030160 AT1G07440.1 27.519 258 176 6 35 288 12 262 1.08e-19 87.0
Msa1030160 AT2G29310.1 26.316 266 170 6 35 288 7 258 1.18e-19 87.0
Msa1030160 AT3G55310.1 27.820 266 181 6 35 292 17 279 1.39e-19 87.0
Msa1030160 AT2G29330.1 26.437 261 176 6 35 288 7 258 2.25e-19 86.3
Msa1030160 AT4G13180.1 29.134 254 168 6 35 281 13 261 3.99e-19 85.5
Msa1030160 AT4G05530.1 28.137 263 172 7 27 283 1 252 6.73e-19 84.7
Msa1030160 AT3G55290.1 26.937 271 177 8 35 292 18 280 1.46e-18 84.3
Msa1030160 AT3G55290.2 27.068 266 183 6 35 292 17 279 1.51e-18 84.3
Msa1030160 AT2G30670.1 27.203 261 174 6 35 288 7 258 1.51e-18 84.0
Msa1030160 AT5G50700.1 28.643 199 134 5 25 219 35 229 2.84e-18 84.3
Msa1030160 AT5G50600.1 28.643 199 134 5 25 219 35 229 2.84e-18 84.3
Msa1030160 AT2G29340.3 29.146 199 134 5 35 229 7 202 6.76e-18 80.9
Msa1030160 AT2G29340.4 29.146 199 134 5 35 229 7 202 6.76e-18 80.9
Msa1030160 AT3G46170.1 28.195 266 180 6 35 292 26 288 9.66e-18 82.0
Msa1030160 AT1G24360.1 25.000 256 177 7 33 282 72 318 4.36e-17 80.9
Msa1030160 AT5G50590.2 31.053 190 125 5 33 219 43 229 1.10e-16 78.6
Msa1030160 AT5G50690.2 31.053 190 125 5 33 219 43 229 1.10e-16 78.6
Msa1030160 AT5G50590.1 31.053 190 125 5 33 219 43 229 2.07e-16 78.6
Msa1030160 AT5G50690.1 31.053 190 125 5 33 219 43 229 2.07e-16 78.6
Msa1030160 AT2G29320.1 25.954 262 177 6 35 288 13 265 2.40e-16 77.8
Msa1030160 AT2G29360.1 26.070 257 182 6 35 288 16 267 2.49e-16 77.8
Msa1030160 AT2G29310.3 28.646 192 132 3 35 223 7 196 6.68e-16 75.5
Msa1030160 AT3G03330.1 30.412 194 127 4 35 221 41 233 1.15e-15 76.6
Msa1030160 AT3G47350.1 28.311 219 138 7 37 249 46 251 1.69e-15 75.9
Msa1030160 AT3G47350.2 28.311 219 138 7 37 249 46 251 2.06e-15 75.9
Msa1030160 AT1G49670.2 31.818 198 121 5 37 221 6 202 2.18e-15 77.0
Msa1030160 AT2G29290.2 26.154 260 178 6 35 288 7 258 2.42e-15 75.1
Msa1030160 AT1G10310.1 30.526 190 129 3 38 226 18 205 2.42e-15 74.7
Msa1030160 AT1G49670.1 31.818 198 121 5 37 221 6 202 2.77e-15 76.6
Msa1030160 AT2G29150.1 26.744 258 178 7 35 288 16 266 2.80e-15 74.7
Msa1030160 AT1G07450.1 27.132 258 177 7 35 288 8 258 4.27e-15 74.3
Msa1030160 AT2G29370.1 27.519 258 177 8 35 288 16 267 4.60e-15 74.3
Msa1030160 AT2G29260.1 26.482 253 174 6 35 282 68 313 5.21e-15 74.7
Msa1030160 AT2G29300.1 24.514 257 185 6 35 287 7 258 6.96e-15 73.6
Msa1030160 AT5G10050.1 28.718 195 131 4 36 228 6 194 7.66e-15 73.9
Msa1030160 AT2G29300.2 24.800 250 179 6 35 280 7 251 1.48e-14 73.2
Msa1030160 AT2G29350.1 27.519 258 176 6 35 288 15 265 2.20e-14 72.4
Msa1030160 AT5G65205.1 30.108 186 122 4 36 219 7 186 3.08e-13 69.3
Msa1030160 AT5G04900.1 29.534 193 126 4 41 224 83 274 1.30e-12 67.8
Msa1030160 AT2G29310.2 25.726 241 151 8 61 288 31 256 1.79e-12 66.6
Msa1030160 AT2G29320.3 28.272 191 131 4 35 221 13 201 4.40e-12 64.7
Msa1030160 AT3G47360.1 28.351 194 127 6 38 225 48 235 4.40e-12 66.2
Msa1030160 AT4G10020.1 26.630 184 123 5 35 211 47 225 3.90e-11 63.5
Msa1030160 AT5G50770.1 27.041 196 131 4 30 219 40 229 7.11e-11 62.8

Find 62 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATTTGACGTTAATGTTTA+TGG 0.157931 7_1:+64098145 Msa1030160:CDS
TCTTCCATGGCTTCAATTAT+TGG 0.161831 7_1:+64097717 Msa1030160:CDS
ATTGATCAGTAGTAAGAATT+TGG 0.166602 7_1:+64098640 Msa1030160:exon
GATACCGTAGTTTCGAATTA+TGG 0.217742 7_1:+64098034 Msa1030160:CDS
AAGAATTTGTGTGTAGAATT+AGG 0.223231 7_1:+64098316 Msa1030160:CDS
AAAATGCTTCATTGCACTTT+TGG 0.251580 7_1:-64098846 None:intergenic
TAATGTTTATGGAGCTTTCT+TGG 0.279073 7_1:+64098156 Msa1030160:CDS
TGTGTAGAATTAGGACAATA+TGG 0.323778 7_1:+64098325 Msa1030160:CDS
ATTAGTAGTTGGAACAGTTT+TGG 0.327434 7_1:+64097552 None:intergenic
TTCAGTGCATTGGTTAGAAT+TGG 0.330425 7_1:-64098388 None:intergenic
TATCATAATTTAATATTTCT+CGG 0.333064 7_1:-64098105 None:intergenic
GAATGGAGTCAACTTTGTTT+TGG 0.335482 7_1:+64098534 Msa1030160:CDS
AGTAAGAATTTGGATTAATT+CGG 0.341144 7_1:+64098650 Msa1030160:exon
CTCTCTTTGTGCAGGCTTGA+AGG 0.373704 7_1:+64097812 Msa1030160:intron
AATGTTTATGGAGCTTTCTT+GGG 0.383944 7_1:+64098157 Msa1030160:CDS
ACAGGAGGTGCTAGTGGAAT+TGG 0.390234 7_1:+64097851 Msa1030160:CDS
CAATCCCTTTGCAATGAACT+TGG 0.395230 7_1:+64097947 Msa1030160:CDS
TCAAACTCTAGTGGAGTTAG+TGG 0.417098 7_1:+64097686 Msa1030160:CDS
AAATGTTTGATGAATGGTTA+TGG 0.432024 7_1:+64098604 Msa1030160:CDS
CTTATAAAATGTTTGATGAA+TGG 0.442320 7_1:+64098598 Msa1030160:CDS
ATGATAGCGAAGCTTTCAAA+AGG 0.448243 7_1:+64098122 Msa1030160:CDS
CTTGTTCATATTCAGTGCAT+TGG 0.455778 7_1:-64098398 None:intergenic
TGGCTTCAATTATTGGCAAA+AGG 0.456299 7_1:+64097724 Msa1030160:CDS
GACATCATGTTTAACAATGC+TGG 0.458821 7_1:+64098064 Msa1030160:CDS
GAATATGAACAAGAGTGAAA+TGG 0.462229 7_1:+64098408 Msa1030160:CDS
TCTTCCAAGTTCATTGCAAA+GGG 0.463133 7_1:-64097951 None:intergenic
TTCTTCCAAGTTCATTGCAA+AGG 0.472083 7_1:-64097952 None:intergenic
AACTGCACTGAACTATTATG+AGG 0.483911 7_1:+64098682 Msa1030160:three_prime_UTR
GATGCTTCAAAGTTTGTGAA+TGG 0.492301 7_1:+64098517 Msa1030160:CDS
TGCAGAAGTGAATGACATAT+TGG 0.498711 7_1:-64098575 None:intergenic
TGGGTGCTAAGCATGCTGCT+AGG 0.498748 7_1:+64098176 Msa1030160:CDS
GTGTAGAATTAGGACAATAT+GGG 0.500905 7_1:+64098326 Msa1030160:CDS
GGGTGCTAAGCATGCTGCTA+GGG 0.521675 7_1:+64098177 Msa1030160:CDS
AGAGGCTATTCTTGAAGTTG+AGG 0.532968 7_1:+64098462 Msa1030160:CDS
CTACTGATCAATGTGGTTGG+TGG 0.535042 7_1:-64098629 None:intergenic
TTTGCCAATAATTGAAGCCA+TGG 0.539515 7_1:-64097721 None:intergenic
TTCTTCAAAGCATGCATCAG+TGG 0.545758 7_1:+64098285 Msa1030160:CDS
GGTAAAGTAGCAATAATCAC+AGG 0.547973 7_1:+64097833 Msa1030160:CDS
AGTGGGAAAATCATCTTCCA+TGG 0.550833 7_1:+64097704 Msa1030160:CDS
TCTTCAAAGCATGCATCAGT+GGG 0.552690 7_1:+64098286 Msa1030160:CDS
TATATATTCATTAATAGTTG+CGG 0.552856 7_1:-64097623 None:intergenic
ATAATCACAGGAGGTGCTAG+TGG 0.563533 7_1:+64097845 Msa1030160:CDS
GCAGATATTCAAGATGAAGT+AGG 0.564586 7_1:+64097923 Msa1030160:CDS
TTACTACTGATCAATGTGGT+TGG 0.572391 7_1:-64098632 None:intergenic
CTCAGTTGGTGTGTTGAAAG+AGG 0.576270 7_1:+64098444 Msa1030160:CDS
CACACTAGTTACAAATCAGA+GGG 0.590387 7_1:-64098880 None:intergenic
TGATAGCGAAGCTTTCAAAA+GGG 0.590961 7_1:+64098123 Msa1030160:CDS
CTTACCATAATTCGAAACTA+CGG 0.593414 7_1:-64098038 None:intergenic
GCATTGGTTAGAATTGGAGT+AGG 0.601696 7_1:-64098382 None:intergenic
CAAACTCTAGTGGAGTTAGT+GGG 0.605035 7_1:+64097687 Msa1030160:CDS
ATAGTTCAGTGCAGTTGTTG+AGG 0.609897 7_1:-64098675 None:intergenic
ATTGGAGTAGGAATAGCTCC+TGG 0.611949 7_1:-64098370 None:intergenic
AAAGTGAATTGTATCTCACC+AGG 0.618157 7_1:+64098352 Msa1030160:CDS
TCACACTAGTTACAAATCAG+AGG 0.623026 7_1:-64098881 None:intergenic
CTCTTTCAACACACCAACTG+AGG 0.623548 7_1:-64098443 None:intergenic
TGAAGTTGAGGATATAGCTG+AGG 0.641258 7_1:+64098474 Msa1030160:CDS
GCTAAACTATTTGTTCAACA+TGG 0.643126 7_1:+64097884 Msa1030160:CDS
AGTGTTGCTACAGAAACTGC+TGG 0.652960 7_1:+64098241 Msa1030160:CDS
AGTTTCTGTAGCAACACTTG+CGG 0.653076 7_1:-64098236 None:intergenic
AGAAGTGAATGACATATTGG+TGG 0.654410 7_1:-64098572 None:intergenic
AAAGTAGCAATAATCACAGG+AGG 0.676889 7_1:+64097836 Msa1030160:CDS
ATTCTTACTACTGATCAATG+TGG 0.704791 7_1:-64098636 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCATAATTTAATATTTCT+CGG - chr7_1:64098108-64098127 None:intergenic 10.0%
!! TATCATAATTTAATATTTCT+CGG - chr7_1:64098108-64098127 None:intergenic 10.0%
!! TATATATTCATTAATAGTTG+CGG - chr7_1:64097626-64097645 None:intergenic 15.0%
!!! ATTTGACTTAATAGTATTTT+TGG + chr7_1:64098752-64098771 Msa1030160:three_prime_UTR 15.0%
!!! AAGAAGTTCAATTTTAAAAA+TGG + chr7_1:64098792-64098811 Msa1030160:three_prime_UTR 15.0%
!!! AATAAGAATCTATGAATTTT+AGG + chr7_1:64098932-64098951 Msa1030160:three_prime_UTR 15.0%
!! TATATATTCATTAATAGTTG+CGG - chr7_1:64097626-64097645 None:intergenic 15.0%
!!! ATTTGACTTAATAGTATTTT+TGG + chr7_1:64098752-64098771 Msa1030160:three_prime_UTR 15.0%
!!! AAGAAGTTCAATTTTAAAAA+TGG + chr7_1:64098792-64098811 Msa1030160:three_prime_UTR 15.0%
!! CTTATAAAATGTTTGATGAA+TGG + chr7_1:64098598-64098617 Msa1030160:CDS 20.0%
!! AGTAAGAATTTGGATTAATT+CGG + chr7_1:64098650-64098669 Msa1030160:exon 20.0%
!!! TTTTATCACTTTAACAAAAG+TGG - chr7_1:64098822-64098841 None:intergenic 20.0%
!! CTTATAAAATGTTTGATGAA+TGG + chr7_1:64098598-64098617 Msa1030160:CDS 20.0%
!! AGTAAGAATTTGGATTAATT+CGG + chr7_1:64098650-64098669 Msa1030160:exon 20.0%
!!! TTTTATCACTTTAACAAAAG+TGG - chr7_1:64098822-64098841 None:intergenic 20.0%
! GTATTTGACGTTAATGTTTA+TGG + chr7_1:64098145-64098164 Msa1030160:CDS 25.0%
!!! GAATAACACCTTTTTTGTTT+GGG - chr7_1:64098210-64098229 None:intergenic 25.0%
!!! AGAATAACACCTTTTTTGTT+TGG - chr7_1:64098211-64098230 None:intergenic 25.0%
! AAGAATTTGTGTGTAGAATT+AGG + chr7_1:64098316-64098335 Msa1030160:CDS 25.0%
! AAATGTTTGATGAATGGTTA+TGG + chr7_1:64098604-64098623 Msa1030160:CDS 25.0%
! ATTGATCAGTAGTAAGAATT+TGG + chr7_1:64098640-64098659 Msa1030160:exon 25.0%
! GTATTTGACGTTAATGTTTA+TGG + chr7_1:64098145-64098164 Msa1030160:CDS 25.0%
!!! GAATAACACCTTTTTTGTTT+GGG - chr7_1:64098210-64098229 None:intergenic 25.0%
!!! AGAATAACACCTTTTTTGTT+TGG - chr7_1:64098211-64098230 None:intergenic 25.0%
! AAGAATTTGTGTGTAGAATT+AGG + chr7_1:64098316-64098335 Msa1030160:CDS 25.0%
! AAATGTTTGATGAATGGTTA+TGG + chr7_1:64098604-64098623 Msa1030160:CDS 25.0%
! ATTGATCAGTAGTAAGAATT+TGG + chr7_1:64098640-64098659 Msa1030160:exon 25.0%
!! AAAAAGCATTCAAACTCTAG+TGG + chr7_1:64097677-64097696 Msa1030160:CDS 30.0%
GCTAAACTATTTGTTCAACA+TGG + chr7_1:64097884-64097903 Msa1030160:CDS 30.0%
CTTACCATAATTCGAAACTA+CGG - chr7_1:64098041-64098060 None:intergenic 30.0%
TAATGTTTATGGAGCTTTCT+TGG + chr7_1:64098156-64098175 Msa1030160:CDS 30.0%
AATGTTTATGGAGCTTTCTT+GGG + chr7_1:64098157-64098176 Msa1030160:CDS 30.0%
GTAATGATCCCAAACAAAAA+AGG + chr7_1:64098199-64098218 Msa1030160:CDS 30.0%
TGTGTAGAATTAGGACAATA+TGG + chr7_1:64098325-64098344 Msa1030160:CDS 30.0%
GTGTAGAATTAGGACAATAT+GGG + chr7_1:64098326-64098345 Msa1030160:CDS 30.0%
GAATATGAACAAGAGTGAAA+TGG + chr7_1:64098408-64098427 Msa1030160:CDS 30.0%
ATTCTTACTACTGATCAATG+TGG - chr7_1:64098639-64098658 None:intergenic 30.0%
! AAAATGCTTCATTGCACTTT+TGG - chr7_1:64098849-64098868 None:intergenic 30.0%
!! AAAAAGCATTCAAACTCTAG+TGG + chr7_1:64097677-64097696 Msa1030160:CDS 30.0%
GCTAAACTATTTGTTCAACA+TGG + chr7_1:64097884-64097903 Msa1030160:CDS 30.0%
CTTACCATAATTCGAAACTA+CGG - chr7_1:64098041-64098060 None:intergenic 30.0%
TAATGTTTATGGAGCTTTCT+TGG + chr7_1:64098156-64098175 Msa1030160:CDS 30.0%
AATGTTTATGGAGCTTTCTT+GGG + chr7_1:64098157-64098176 Msa1030160:CDS 30.0%
GTAATGATCCCAAACAAAAA+AGG + chr7_1:64098199-64098218 Msa1030160:CDS 30.0%
TGTGTAGAATTAGGACAATA+TGG + chr7_1:64098325-64098344 Msa1030160:CDS 30.0%
GTGTAGAATTAGGACAATAT+GGG + chr7_1:64098326-64098345 Msa1030160:CDS 30.0%
GAATATGAACAAGAGTGAAA+TGG + chr7_1:64098408-64098427 Msa1030160:CDS 30.0%
ATTCTTACTACTGATCAATG+TGG - chr7_1:64098639-64098658 None:intergenic 30.0%
! AAAATGCTTCATTGCACTTT+TGG - chr7_1:64098849-64098868 None:intergenic 30.0%
TCTTCCATGGCTTCAATTAT+TGG + chr7_1:64097717-64097736 Msa1030160:CDS 35.0%
TTTGCCAATAATTGAAGCCA+TGG - chr7_1:64097724-64097743 None:intergenic 35.0%
TGGCTTCAATTATTGGCAAA+AGG + chr7_1:64097724-64097743 Msa1030160:CDS 35.0%
GGCAAAAGGTAACTATATTG+TGG + chr7_1:64097738-64097757 Msa1030160:intron 35.0%
GGTAAAGTAGCAATAATCAC+AGG + chr7_1:64097833-64097852 Msa1030160:CDS 35.0%
AAAGTAGCAATAATCACAGG+AGG + chr7_1:64097836-64097855 Msa1030160:CDS 35.0%
GCAGATATTCAAGATGAAGT+AGG + chr7_1:64097923-64097942 Msa1030160:CDS 35.0%
TCTTCCAAGTTCATTGCAAA+GGG - chr7_1:64097954-64097973 None:intergenic 35.0%
TTCTTCCAAGTTCATTGCAA+AGG - chr7_1:64097955-64097974 None:intergenic 35.0%
GATACCGTAGTTTCGAATTA+TGG + chr7_1:64098034-64098053 Msa1030160:CDS 35.0%
GACATCATGTTTAACAATGC+TGG + chr7_1:64098064-64098083 Msa1030160:CDS 35.0%
! ATGATAGCGAAGCTTTCAAA+AGG + chr7_1:64098122-64098141 Msa1030160:CDS 35.0%
! TGATAGCGAAGCTTTCAAAA+GGG + chr7_1:64098123-64098142 Msa1030160:CDS 35.0%
AAAGTGAATTGTATCTCACC+AGG + chr7_1:64098352-64098371 Msa1030160:CDS 35.0%
!! TTCAGTGCATTGGTTAGAAT+TGG - chr7_1:64098391-64098410 None:intergenic 35.0%
CTTGTTCATATTCAGTGCAT+TGG - chr7_1:64098401-64098420 None:intergenic 35.0%
GATGCTTCAAAGTTTGTGAA+TGG + chr7_1:64098517-64098536 Msa1030160:CDS 35.0%
!! GAATGGAGTCAACTTTGTTT+TGG + chr7_1:64098534-64098553 Msa1030160:CDS 35.0%
AGAAGTGAATGACATATTGG+TGG - chr7_1:64098575-64098594 None:intergenic 35.0%
TGCAGAAGTGAATGACATAT+TGG - chr7_1:64098578-64098597 None:intergenic 35.0%
TTACTACTGATCAATGTGGT+TGG - chr7_1:64098635-64098654 None:intergenic 35.0%
AACTGCACTGAACTATTATG+AGG + chr7_1:64098682-64098701 Msa1030160:three_prime_UTR 35.0%
CACACTAGTTACAAATCAGA+GGG - chr7_1:64098883-64098902 None:intergenic 35.0%
TCACACTAGTTACAAATCAG+AGG - chr7_1:64098884-64098903 None:intergenic 35.0%
TCTTCCATGGCTTCAATTAT+TGG + chr7_1:64097717-64097736 Msa1030160:CDS 35.0%
TTTGCCAATAATTGAAGCCA+TGG - chr7_1:64097724-64097743 None:intergenic 35.0%
TGGCTTCAATTATTGGCAAA+AGG + chr7_1:64097724-64097743 Msa1030160:CDS 35.0%
GGCAAAAGGTAACTATATTG+TGG + chr7_1:64097738-64097757 Msa1030160:intron 35.0%
GGTAAAGTAGCAATAATCAC+AGG + chr7_1:64097833-64097852 Msa1030160:CDS 35.0%
AAAGTAGCAATAATCACAGG+AGG + chr7_1:64097836-64097855 Msa1030160:CDS 35.0%
GCAGATATTCAAGATGAAGT+AGG + chr7_1:64097923-64097942 Msa1030160:CDS 35.0%
TCTTCCAAGTTCATTGCAAA+GGG - chr7_1:64097954-64097973 None:intergenic 35.0%
TTCTTCCAAGTTCATTGCAA+AGG - chr7_1:64097955-64097974 None:intergenic 35.0%
GATACCGTAGTTTCGAATTA+TGG + chr7_1:64098034-64098053 Msa1030160:CDS 35.0%
GACATCATGTTTAACAATGC+TGG + chr7_1:64098064-64098083 Msa1030160:CDS 35.0%
! ATGATAGCGAAGCTTTCAAA+AGG + chr7_1:64098122-64098141 Msa1030160:CDS 35.0%
! TGATAGCGAAGCTTTCAAAA+GGG + chr7_1:64098123-64098142 Msa1030160:CDS 35.0%
AAAGTGAATTGTATCTCACC+AGG + chr7_1:64098352-64098371 Msa1030160:CDS 35.0%
!! TTCAGTGCATTGGTTAGAAT+TGG - chr7_1:64098391-64098410 None:intergenic 35.0%
CTTGTTCATATTCAGTGCAT+TGG - chr7_1:64098401-64098420 None:intergenic 35.0%
GATGCTTCAAAGTTTGTGAA+TGG + chr7_1:64098517-64098536 Msa1030160:CDS 35.0%
!! GAATGGAGTCAACTTTGTTT+TGG + chr7_1:64098534-64098553 Msa1030160:CDS 35.0%
AGAAGTGAATGACATATTGG+TGG - chr7_1:64098575-64098594 None:intergenic 35.0%
TGCAGAAGTGAATGACATAT+TGG - chr7_1:64098578-64098597 None:intergenic 35.0%
TTACTACTGATCAATGTGGT+TGG - chr7_1:64098635-64098654 None:intergenic 35.0%
AACTGCACTGAACTATTATG+AGG + chr7_1:64098682-64098701 Msa1030160:three_prime_UTR 35.0%
CACACTAGTTACAAATCAGA+GGG - chr7_1:64098883-64098902 None:intergenic 35.0%
TCACACTAGTTACAAATCAG+AGG - chr7_1:64098884-64098903 None:intergenic 35.0%
TCAAACTCTAGTGGAGTTAG+TGG + chr7_1:64097686-64097705 Msa1030160:CDS 40.0%
CAAACTCTAGTGGAGTTAGT+GGG + chr7_1:64097687-64097706 Msa1030160:CDS 40.0%
AGTGGGAAAATCATCTTCCA+TGG + chr7_1:64097704-64097723 Msa1030160:CDS 40.0%
CAAGTAATCTCTCTTTGTGC+AGG + chr7_1:64097804-64097823 Msa1030160:intron 40.0%
CAATCCCTTTGCAATGAACT+TGG + chr7_1:64097947-64097966 Msa1030160:CDS 40.0%
AGTTTCTGTAGCAACACTTG+CGG - chr7_1:64098239-64098258 None:intergenic 40.0%
TTCTTCAAAGCATGCATCAG+TGG + chr7_1:64098285-64098304 Msa1030160:CDS 40.0%
TCTTCAAAGCATGCATCAGT+GGG + chr7_1:64098286-64098305 Msa1030160:CDS 40.0%
!! GCATTGGTTAGAATTGGAGT+AGG - chr7_1:64098385-64098404 None:intergenic 40.0%
!! GAGAAAGTTTTGTCCTCAGT+TGG + chr7_1:64098430-64098449 Msa1030160:CDS 40.0%
AGAGGCTATTCTTGAAGTTG+AGG + chr7_1:64098462-64098481 Msa1030160:CDS 40.0%
TGAAGTTGAGGATATAGCTG+AGG + chr7_1:64098474-64098493 Msa1030160:CDS 40.0%
!!! GGAGTCAACTTTGTTTTGGA+TGG + chr7_1:64098538-64098557 Msa1030160:CDS 40.0%
!!! GTCAACTTTGTTTTGGATGG+AGG + chr7_1:64098541-64098560 Msa1030160:CDS 40.0%
ATAGTTCAGTGCAGTTGTTG+AGG - chr7_1:64098678-64098697 None:intergenic 40.0%
TCAAACTCTAGTGGAGTTAG+TGG + chr7_1:64097686-64097705 Msa1030160:CDS 40.0%
CAAACTCTAGTGGAGTTAGT+GGG + chr7_1:64097687-64097706 Msa1030160:CDS 40.0%
AGTGGGAAAATCATCTTCCA+TGG + chr7_1:64097704-64097723 Msa1030160:CDS 40.0%
CAAGTAATCTCTCTTTGTGC+AGG + chr7_1:64097804-64097823 Msa1030160:intron 40.0%
CAATCCCTTTGCAATGAACT+TGG + chr7_1:64097947-64097966 Msa1030160:CDS 40.0%
AGTTTCTGTAGCAACACTTG+CGG - chr7_1:64098239-64098258 None:intergenic 40.0%
TTCTTCAAAGCATGCATCAG+TGG + chr7_1:64098285-64098304 Msa1030160:CDS 40.0%
TCTTCAAAGCATGCATCAGT+GGG + chr7_1:64098286-64098305 Msa1030160:CDS 40.0%
!! GCATTGGTTAGAATTGGAGT+AGG - chr7_1:64098385-64098404 None:intergenic 40.0%
!! GAGAAAGTTTTGTCCTCAGT+TGG + chr7_1:64098430-64098449 Msa1030160:CDS 40.0%
AGAGGCTATTCTTGAAGTTG+AGG + chr7_1:64098462-64098481 Msa1030160:CDS 40.0%
TGAAGTTGAGGATATAGCTG+AGG + chr7_1:64098474-64098493 Msa1030160:CDS 40.0%
!!! GGAGTCAACTTTGTTTTGGA+TGG + chr7_1:64098538-64098557 Msa1030160:CDS 40.0%
!!! GTCAACTTTGTTTTGGATGG+AGG + chr7_1:64098541-64098560 Msa1030160:CDS 40.0%
ATAGTTCAGTGCAGTTGTTG+AGG - chr7_1:64098678-64098697 None:intergenic 40.0%
!! ATAATCACAGGAGGTGCTAG+TGG + chr7_1:64097845-64097864 Msa1030160:CDS 45.0%
! AGTGTTGCTACAGAAACTGC+TGG + chr7_1:64098241-64098260 Msa1030160:CDS 45.0%
ATTGGAGTAGGAATAGCTCC+TGG - chr7_1:64098373-64098392 None:intergenic 45.0%
CTCTTTCAACACACCAACTG+AGG - chr7_1:64098446-64098465 None:intergenic 45.0%
! CTCAGTTGGTGTGTTGAAAG+AGG + chr7_1:64098444-64098463 Msa1030160:CDS 45.0%
CTACTGATCAATGTGGTTGG+TGG - chr7_1:64098632-64098651 None:intergenic 45.0%
!! ATAATCACAGGAGGTGCTAG+TGG + chr7_1:64097845-64097864 Msa1030160:CDS 45.0%
! AGTGTTGCTACAGAAACTGC+TGG + chr7_1:64098241-64098260 Msa1030160:CDS 45.0%
ATTGGAGTAGGAATAGCTCC+TGG - chr7_1:64098373-64098392 None:intergenic 45.0%
CTCTTTCAACACACCAACTG+AGG - chr7_1:64098446-64098465 None:intergenic 45.0%
! CTCAGTTGGTGTGTTGAAAG+AGG + chr7_1:64098444-64098463 Msa1030160:CDS 45.0%
CTACTGATCAATGTGGTTGG+TGG - chr7_1:64098632-64098651 None:intergenic 45.0%
CTCTCTTTGTGCAGGCTTGA+AGG + chr7_1:64097812-64097831 Msa1030160:intron 50.0%
!! ACAGGAGGTGCTAGTGGAAT+TGG + chr7_1:64097851-64097870 Msa1030160:CDS 50.0%
CTCTCTTTGTGCAGGCTTGA+AGG + chr7_1:64097812-64097831 Msa1030160:intron 50.0%
!! ACAGGAGGTGCTAGTGGAAT+TGG + chr7_1:64097851-64097870 Msa1030160:CDS 50.0%
TGGGTGCTAAGCATGCTGCT+AGG + chr7_1:64098176-64098195 Msa1030160:CDS 55.0%
GGGTGCTAAGCATGCTGCTA+GGG + chr7_1:64098177-64098196 Msa1030160:CDS 55.0%
TGGGTGCTAAGCATGCTGCT+AGG + chr7_1:64098176-64098195 Msa1030160:CDS 55.0%
GGGTGCTAAGCATGCTGCTA+GGG + chr7_1:64098177-64098196 Msa1030160:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 64097562 64098952 64097562 ID=Msa1030160;Name=Msa1030160
chr7_1 mRNA 64097562 64098952 64097562 ID=Msa1030160-mRNA-1;Parent=Msa1030160;Name=Msa1030160-mRNA-1;_AED=0.02;_eAED=0.02;_QI=83|1|1|1|1|1|2|301|308
chr7_1 exon 64097562 64097745 64097562 ID=Msa1030160-mRNA-1:exon:14006;Parent=Msa1030160-mRNA-1
chr7_1 exon 64097826 64098952 64097826 ID=Msa1030160-mRNA-1:exon:14007;Parent=Msa1030160-mRNA-1
chr7_1 five_prime_UTR 64097562 64097644 64097562 ID=Msa1030160-mRNA-1:five_prime_utr;Parent=Msa1030160-mRNA-1
chr7_1 CDS 64097645 64097745 64097645 ID=Msa1030160-mRNA-1:cds;Parent=Msa1030160-mRNA-1
chr7_1 CDS 64097826 64098651 64097826 ID=Msa1030160-mRNA-1:cds;Parent=Msa1030160-mRNA-1
chr7_1 three_prime_UTR 64098652 64098952 64098652 ID=Msa1030160-mRNA-1:three_prime_utr;Parent=Msa1030160-mRNA-1
Gene Sequence

>Msa1030160

ATGCATTTAAAAAAACAAAAATCTGCTACTATAAAAAGCATTCAAACTCTAGTGGAGTTAGTGGGAAAATCATCTTCCATGGCTTCAATTATTGGCAAAAGGCTTGAAGGTAAAGTAGCAATAATCACAGGAGGTGCTAGTGGAATTGGTGCAGCTACTGCTAAACTATTTGTTCAACATGGTGCTAAAGTTGTGATTGCAGATATTCAAGATGAAGTAGGTCAATCCCTTTGCAATGAACTTGGAAGAAAGAACATTCTCTATGTTCACTGTAATGTAACTATTGAATCAGACATAAAAAACGTTGTTGATACCGTAGTTTCGAATTATGGTAAGCTTGACATCATGTTTAACAATGCTGGTATCTCAGATGACAAAAACCGAGAAATATTAAATTATGATAGCGAAGCTTTCAAAAGGGTATTTGACGTTAATGTTTATGGAGCTTTCTTGGGTGCTAAGCATGCTGCTAGGGTAATGATCCCAAACAAAAAAGGTGTTATTCTTTTTACCGCAAGTGTTGCTACAGAAACTGCTGGTGAATCAACACATGCTTATTCTTCTTCAAAGCATGCATCAGTGGGACTTATGAAGAATTTGTGTGTAGAATTAGGACAATATGGGATTAAAGTGAATTGTATCTCACCAGGAGCTATTCCTACTCCAATTCTAACCAATGCACTGAATATGAACAAGAGTGAAATGGAGAAAGTTTTGTCCTCAGTTGGTGTGTTGAAAGAGGCTATTCTTGAAGTTGAGGATATAGCTGAGGCAGCACTTTATTTGAGCAGTGATGCTTCAAAGTTTGTGAATGGAGTCAACTTTGTTTTGGATGGAGGTTATAGCACCACCAATATGTCATTCACTTCTGCACTTATAAAATGTTTGATGAATGGTTATGGCACCACCAACCACATTGATCAGTAG
Protein sequence

>Msa1030160

MHLKKQKSATIKSIQTLVELVGKSSSMASIIGKRLEGKVAIITGGASGIGAATAKLFVQHGAKVVIADIQDEVGQSLCNELGRKNILYVHCNVTIESDIKNVVDTVVSNYGKLDIMFNNAGISDDKNREILNYDSEAFKRVFDVNVYGAFLGAKHAARVMIPNKKGVILFTASVATETAGESTHAYSSSKHASVGLMKNLCVELGQYGIKVNCISPGAIPTPILTNALNMNKSEMEKVLSSVGVLKEAILEVEDIAEAALYLSSDASKFVNGVNFVLDGGYSTTNMSFTSALIKCLMNGYGTTNHIDQ*