AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1034330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1034330 MtrunA17_Chr7g0257181 100.000 68 0 0 38 105 1 68 1.74e-44 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1034330 AT2G43780.4 66.197 71 23 1 35 105 24 93 4.13e-26 93.2
Msa1034330 AT2G43780.3 68.657 67 20 1 38 104 1 66 9.19e-26 91.7
Msa1034330 AT2G43780.1 68.657 67 20 1 38 104 1 66 9.19e-26 91.7
Msa1034330 AT2G43780.2 68.657 67 20 1 38 104 1 66 9.19e-26 91.7

Find 48 sgRNAs with CRISPR-Local

Find 312 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTAATACTCCAGCCATTT+TGG 0.197201 7_1:+69800376 None:intergenic
CGAGGTGGACGCGGCGGTCT+TGG 0.201704 7_1:+69801925 None:intergenic
TTCTTTATAGTTTACCAAAA+TGG 0.227595 7_1:-69800390 Msa1034330:intron
AATGCTTGATTCGCCTTCTT+TGG 0.262054 7_1:+69800202 None:intergenic
GTCGCTTTCTCTTTCTCTTC+TGG 0.274508 7_1:-69801900 Msa1034330:intron
CCTTGTATTTAATAAAGTTT+AGG 0.300367 7_1:-69800051 Msa1034330:three_prime_UTR
ACTATGAAATTCTTTGTCTT+AGG 0.312137 7_1:+69800338 None:intergenic
ACCAAAATGGCTGGAGTATT+AGG 0.318096 7_1:-69800377 Msa1034330:CDS
GCCTTCTTTGGTGCTCTTGT+CGG 0.348229 7_1:+69800214 None:intergenic
AACGGTTATTATGTTTCAAT+TGG 0.349565 7_1:+69799991 None:intergenic
GAGGTGGACGCGGCGGTCTT+GGG 0.355664 7_1:+69801926 None:intergenic
TTTGGTTGTACGCAATGTTT+TGG 0.369171 7_1:-69800133 Msa1034330:three_prime_UTR
ACAAAGAATTTCATAGTTGC+CGG 0.374694 7_1:-69800332 Msa1034330:CDS
ACTAAGCTCTAGGCCTTTGA+TGG 0.419130 7_1:+69800179 None:intergenic
CCTAAACTTTATTAAATACA+AGG 0.423444 7_1:+69800051 None:intergenic
AATAACATGAACAGTACTTA+AGG 0.452950 7_1:+69800016 None:intergenic
TTATAGTTTACCAAAATGGC+TGG 0.453643 7_1:-69800386 Msa1034330:intron
ACAGTACACAAATAATACTT+TGG 0.456449 7_1:-69800151 Msa1034330:three_prime_UTR
TAACTATTTCCATGCTGAAA+GGG 0.462522 7_1:+69800089 None:intergenic
ACGTGAGACTCAACATCTCT+TGG 0.465457 7_1:-69799954 Msa1034330:three_prime_UTR
AAGCGACGGCGAGGTGGACG+CGG 0.486196 7_1:+69801916 None:intergenic
CAAAGAATTTCATAGTTGCC+GGG 0.488180 7_1:-69800331 Msa1034330:CDS
AATTCTTTGTCTTAGGCGCC+AGG 0.505757 7_1:+69800345 None:intergenic
AGTACCTCCAACTGCTCTCA+TGG 0.506868 7_1:+69800271 None:intergenic
GCTGGAGTATTAGGATATAG+TGG 0.507617 7_1:-69800368 Msa1034330:CDS
TTAACTATTTCCATGCTGAA+AGG 0.507702 7_1:+69800088 None:intergenic
CGAATCAAGCATTCCATCAA+AGG 0.507874 7_1:-69800192 Msa1034330:CDS
GCCGACAAGAGCACCAAAGA+AGG 0.517286 7_1:-69800215 Msa1034330:CDS
TTCTACACCATGAGAGCAGT+TGG 0.517929 7_1:-69800278 Msa1034330:CDS
AATTGAAACATAATAACCGT+TGG 0.524572 7_1:-69799989 Msa1034330:three_prime_UTR
TGGAGGTACTGATGAACTAC+AGG 0.526085 7_1:-69800258 Msa1034330:CDS
TCACTAAAACGCCGCCGTAG+AGG 0.561288 7_1:-69801950 Msa1034330:CDS
GTAATATGTAAGACGATGAA+AGG 0.564304 7_1:+69799869 None:intergenic
AACTATTTCCATGCTGAAAG+GGG 0.565638 7_1:+69800090 None:intergenic
ACTGTAAGCAACTAAGCTCT+AGG 0.568639 7_1:+69800169 None:intergenic
CGGCGGTCTTGGGTCCTCTA+CGG 0.574685 7_1:+69801936 None:intergenic
GACAAAAGTTGTCAAACCCC+CGG 0.577777 7_1:+69800313 None:intergenic
AAGAGAAAGCGACGGCGAGG+TGG 0.591651 7_1:+69801910 None:intergenic
AGAAGAGAAAGAGAAAGCGA+CGG 0.598553 7_1:+69801902 None:intergenic
AGAAAGAGAAAGCGACGGCG+AGG 0.608606 7_1:+69801907 None:intergenic
TACACCATGAGAGCAGTTGG+AGG 0.610981 7_1:-69800275 Msa1034330:CDS
AGTATTAGGATATAGTGGCC+TGG 0.611243 7_1:-69800363 Msa1034330:CDS
AAAGAATTTCATAGTTGCCG+GGG 0.615205 7_1:-69800330 Msa1034330:CDS
ACGTGAAATACTTAATCCAA+CGG 0.624324 7_1:+69799973 None:intergenic
CGACGGCGAGGTGGACGCGG+CGG 0.634918 7_1:+69801919 None:intergenic
AAGAATTTCATAGTTGCCGG+GGG 0.655049 7_1:-69800329 Msa1034330:CDS
AAAATGAAGTGCGACAAATG+GGG 0.685319 7_1:+69799893 None:intergenic
CGGTCTTGGGTCCTCTACGG+CGG 0.699173 7_1:+69801939 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGAAAGAAAAAAAAATA+GGG + chr7_1:69800268-69800287 None:intergenic 10.0%
!! AATTTGAAAGAAAAAAAAAT+AGG + chr7_1:69800269-69800288 None:intergenic 10.0%
!!! TTTTTTTTTCTTTCAAATTT+CGG - chr7_1:69800270-69800289 Msa1034330:CDS 10.0%
!! ATTTGAAAGAAAAAAAAATA+GGG + chr7_1:69800268-69800287 None:intergenic 10.0%
!! AATTTGAAAGAAAAAAAAAT+AGG + chr7_1:69800269-69800288 None:intergenic 10.0%
!!! TTTTTTTTTCTTTCAAATTT+CGG - chr7_1:69800270-69800289 Msa1034330:CDS 10.0%
!!! TTCTACAATTTCTAAAAAAA+GGG + chr7_1:69801270-69801289 None:intergenic 15.0%
!!! TTTCTACAATTTCTAAAAAA+AGG + chr7_1:69801271-69801290 None:intergenic 15.0%
!! TAGATTGTGTTAAAAAAATT+GGG - chr7_1:69802148-69802167 Msa1034330:intron 15.0%
!!! TTCTACAATTTCTAAAAAAA+GGG + chr7_1:69801270-69801289 None:intergenic 15.0%
!!! TTTCTACAATTTCTAAAAAA+AGG + chr7_1:69801271-69801290 None:intergenic 15.0%
!! TAGATTGTGTTAAAAAAATT+GGG - chr7_1:69802148-69802167 Msa1034330:intron 15.0%
!! TTTGCTAATATTCCAATTTA+AGG - chr7_1:69800428-69800447 Msa1034330:intron 20.0%
!! AAGCTTAAAAGATTTCAAAA+TGG + chr7_1:69800653-69800672 None:intergenic 20.0%
!! TGTAAATCATAAAAGTTGTT+AGG - chr7_1:69800740-69800759 Msa1034330:intron 20.0%
!! AACTTAGTTTAAAAAACAAG+AGG - chr7_1:69800854-69800873 Msa1034330:intron 20.0%
!!! GAGATTTTAATTGTATTATG+TGG - chr7_1:69801014-69801033 Msa1034330:intron 20.0%
!! TATATCTACAAGATTAGATT+AGG - chr7_1:69801153-69801172 Msa1034330:intron 20.0%
!! AAATAGTTAAAATACTGCAT+TGG + chr7_1:69801320-69801339 None:intergenic 20.0%
!!! ATGCAGTATTTTAACTATTT+AGG - chr7_1:69801320-69801339 Msa1034330:intron 20.0%
!!! TGATTTGTAATCTTTTTACA+AGG - chr7_1:69801426-69801445 Msa1034330:intron 20.0%
!! TGATATATATTACAATCCAA+TGG + chr7_1:69801467-69801486 None:intergenic 20.0%
!! TTCTTTATAGTTTACCAAAA+TGG - chr7_1:69801552-69801571 Msa1034330:intron 20.0%
!! CCTAAACTTTATTAAATACA+AGG + chr7_1:69801894-69801913 None:intergenic 20.0%
!! CCTTGTATTTAATAAAGTTT+AGG - chr7_1:69801891-69801910 Msa1034330:intron 20.0%
!! CTAGATTGTGTTAAAAAAAT+TGG - chr7_1:69802147-69802166 Msa1034330:intron 20.0%
!! TTTGCTAATATTCCAATTTA+AGG - chr7_1:69800428-69800447 Msa1034330:intron 20.0%
!! AAGCTTAAAAGATTTCAAAA+TGG + chr7_1:69800653-69800672 None:intergenic 20.0%
!! TGTAAATCATAAAAGTTGTT+AGG - chr7_1:69800740-69800759 Msa1034330:intron 20.0%
!! AACTTAGTTTAAAAAACAAG+AGG - chr7_1:69800854-69800873 Msa1034330:intron 20.0%
!!! GAGATTTTAATTGTATTATG+TGG - chr7_1:69801014-69801033 Msa1034330:intron 20.0%
!! TATATCTACAAGATTAGATT+AGG - chr7_1:69801153-69801172 Msa1034330:intron 20.0%
!! AAATAGTTAAAATACTGCAT+TGG + chr7_1:69801320-69801339 None:intergenic 20.0%
!!! ATGCAGTATTTTAACTATTT+AGG - chr7_1:69801320-69801339 Msa1034330:intron 20.0%
!!! TGATTTGTAATCTTTTTACA+AGG - chr7_1:69801426-69801445 Msa1034330:intron 20.0%
!! TGATATATATTACAATCCAA+TGG + chr7_1:69801467-69801486 None:intergenic 20.0%
!! TTCTTTATAGTTTACCAAAA+TGG - chr7_1:69801552-69801571 Msa1034330:intron 20.0%
!! CCTAAACTTTATTAAATACA+AGG + chr7_1:69801894-69801913 None:intergenic 20.0%
!! CCTTGTATTTAATAAAGTTT+AGG - chr7_1:69801891-69801910 Msa1034330:intron 20.0%
!! CTAGATTGTGTTAAAAAAAT+TGG - chr7_1:69802147-69802166 Msa1034330:intron 20.0%
! AATTTAACCGAATAAAACAG+AGG - chr7_1:69799809-69799828 Msa1034330:three_prime_UTR 25.0%
! GAACAAAAGATATAATCCAA+AGG + chr7_1:69799835-69799854 None:intergenic 25.0%
! ATTCAAGTATCAGAACTATA+GGG + chr7_1:69800120-69800139 None:intergenic 25.0%
!! GAATGTTTTTCTTTCAATCT+AGG - chr7_1:69800142-69800161 Msa1034330:three_prime_UTR 25.0%
!! TTTTCTTTCAATCTAGGAAA+TGG - chr7_1:69800148-69800167 Msa1034330:three_prime_UTR 25.0%
! TGATTGTTAGAATCTCTAAT+TGG - chr7_1:69800306-69800325 Msa1034330:CDS 25.0%
! TTGAATAGGAATATGATTTC+TGG + chr7_1:69800505-69800524 None:intergenic 25.0%
! ATCATAAAAGTTGTTAGGTA+AGG - chr7_1:69800745-69800764 Msa1034330:intron 25.0%
! CAATATTTCGATCTTACAAA+GGG - chr7_1:69801224-69801243 Msa1034330:intron 25.0%
!! TAACTATTTAGGACTATTTG+AGG - chr7_1:69801331-69801350 Msa1034330:intron 25.0%
!! TTTGTAATCTTTTTACAAGG+TGG - chr7_1:69801429-69801448 Msa1034330:intron 25.0%
!! TTGTAATCTTTTTACAAGGT+GGG - chr7_1:69801430-69801449 Msa1034330:intron 25.0%
! ACTATGAAATTCTTTGTCTT+AGG + chr7_1:69801607-69801626 None:intergenic 25.0%
! ACAGTACACAAATAATACTT+TGG - chr7_1:69801791-69801810 Msa1034330:intron 25.0%
! AATAACATGAACAGTACTTA+AGG + chr7_1:69801929-69801948 None:intergenic 25.0%
! AACGGTTATTATGTTTCAAT+TGG + chr7_1:69801954-69801973 None:intergenic 25.0%
! AATTGAAACATAATAACCGT+TGG - chr7_1:69801953-69801972 Msa1034330:CDS 25.0%
! AATTTAACCGAATAAAACAG+AGG - chr7_1:69799809-69799828 Msa1034330:three_prime_UTR 25.0%
! GAACAAAAGATATAATCCAA+AGG + chr7_1:69799835-69799854 None:intergenic 25.0%
! ATTCAAGTATCAGAACTATA+GGG + chr7_1:69800120-69800139 None:intergenic 25.0%
!! GAATGTTTTTCTTTCAATCT+AGG - chr7_1:69800142-69800161 Msa1034330:three_prime_UTR 25.0%
!! TTTTCTTTCAATCTAGGAAA+TGG - chr7_1:69800148-69800167 Msa1034330:three_prime_UTR 25.0%
! TGATTGTTAGAATCTCTAAT+TGG - chr7_1:69800306-69800325 Msa1034330:CDS 25.0%
! TTGAATAGGAATATGATTTC+TGG + chr7_1:69800505-69800524 None:intergenic 25.0%
! ATCATAAAAGTTGTTAGGTA+AGG - chr7_1:69800745-69800764 Msa1034330:intron 25.0%
! CAATATTTCGATCTTACAAA+GGG - chr7_1:69801224-69801243 Msa1034330:intron 25.0%
!! TAACTATTTAGGACTATTTG+AGG - chr7_1:69801331-69801350 Msa1034330:intron 25.0%
!! TTTGTAATCTTTTTACAAGG+TGG - chr7_1:69801429-69801448 Msa1034330:intron 25.0%
!! TTGTAATCTTTTTACAAGGT+GGG - chr7_1:69801430-69801449 Msa1034330:intron 25.0%
! ACTATGAAATTCTTTGTCTT+AGG + chr7_1:69801607-69801626 None:intergenic 25.0%
! ACAGTACACAAATAATACTT+TGG - chr7_1:69801791-69801810 Msa1034330:intron 25.0%
! AATAACATGAACAGTACTTA+AGG + chr7_1:69801929-69801948 None:intergenic 25.0%
! AACGGTTATTATGTTTCAAT+TGG + chr7_1:69801954-69801973 None:intergenic 25.0%
! AATTGAAACATAATAACCGT+TGG - chr7_1:69801953-69801972 Msa1034330:CDS 25.0%
!! TCCAAAAGTATTACATCCAA+GGG + chr7_1:69799862-69799881 None:intergenic 30.0%
!! ATCCAAAAGTATTACATCCA+AGG + chr7_1:69799863-69799882 None:intergenic 30.0%
!!! GAGTTTTTTGTGAAATGAAG+TGG + chr7_1:69799974-69799993 None:intergenic 30.0%
GTACAAAATAGCGAAAAGAA+GGG + chr7_1:69800084-69800103 None:intergenic 30.0%
GATTCAAGTATCAGAACTAT+AGG + chr7_1:69800121-69800140 None:intergenic 30.0%
TAGTAGACTGTCCAATTTAT+TGG - chr7_1:69800363-69800382 Msa1034330:CDS 30.0%
ATTCGATGTCATAAACAGTT+TGG - chr7_1:69800548-69800567 Msa1034330:intron 30.0%
! TGTCTTTTGTGTATATGACT+TGG - chr7_1:69800617-69800636 Msa1034330:intron 30.0%
! GTCTTTTGTGTATATGACTT+GGG - chr7_1:69800618-69800637 Msa1034330:intron 30.0%
TTGATTACAGCTTGTATGTT+TGG - chr7_1:69800678-69800697 Msa1034330:intron 30.0%
AACGACAATCGAAATCATAA+TGG - chr7_1:69800710-69800729 Msa1034330:intron 30.0%
CATATATACACATACCACTT+TGG + chr7_1:69800806-69800825 None:intergenic 30.0%
AGTGGTATGTGTATATATGT+AGG - chr7_1:69800807-69800826 Msa1034330:intron 30.0%
ATGTGTATATATGTAGGTAC+AGG - chr7_1:69800813-69800832 Msa1034330:intron 30.0%
TGTGTATATATGTAGGTACA+GGG - chr7_1:69800814-69800833 Msa1034330:intron 30.0%
ATTTCTTACTGCTCTCAATT+TGG + chr7_1:69800934-69800953 None:intergenic 30.0%
TAATCTACTTAAGCACACTT+TGG + chr7_1:69801111-69801130 None:intergenic 30.0%
AAAGTGTGCTTAAGTAGATT+AGG - chr7_1:69801110-69801129 Msa1034330:intron 30.0%
CCTTTGTAAGATCGAAATAT+TGG + chr7_1:69801226-69801245 None:intergenic 30.0%
CCAATATTTCGATCTTACAA+AGG - chr7_1:69801223-69801242 Msa1034330:intron 30.0%
TGTAGAAAGAATTATGCATG+AGG - chr7_1:69801283-69801302 Msa1034330:intron 30.0%
AAAGATGGTGACAATTGAAT+GGG - chr7_1:69801363-69801382 Msa1034330:intron 30.0%
GAATTAAACATTCATGTTGC+TGG - chr7_1:69801403-69801422 Msa1034330:intron 30.0%
TTATAGTTTACCAAAATGGC+TGG - chr7_1:69801556-69801575 Msa1034330:intron 30.0%
ACAAAGAATTTCATAGTTGC+CGG - chr7_1:69801610-69801629 Msa1034330:intron 30.0%
! GTTTGACAACTTTTGTCTTT+GGG - chr7_1:69801635-69801654 Msa1034330:intron 30.0%
! TTTGACAACTTTTGTCTTTG+GGG - chr7_1:69801636-69801655 Msa1034330:intron 30.0%
TAACTATTTCCATGCTGAAA+GGG + chr7_1:69801856-69801875 None:intergenic 30.0%
TTAACTATTTCCATGCTGAA+AGG + chr7_1:69801857-69801876 None:intergenic 30.0%
ACGTGAAATACTTAATCCAA+CGG + chr7_1:69801972-69801991 None:intergenic 30.0%
AAAAATGAAGTGCGACAAAT+GGG + chr7_1:69802053-69802072 None:intergenic 30.0%
GTAATATGTAAGACGATGAA+AGG + chr7_1:69802076-69802095 None:intergenic 30.0%
!! TCCAAAAGTATTACATCCAA+GGG + chr7_1:69799862-69799881 None:intergenic 30.0%
!! ATCCAAAAGTATTACATCCA+AGG + chr7_1:69799863-69799882 None:intergenic 30.0%
!!! GAGTTTTTTGTGAAATGAAG+TGG + chr7_1:69799974-69799993 None:intergenic 30.0%
GTACAAAATAGCGAAAAGAA+GGG + chr7_1:69800084-69800103 None:intergenic 30.0%
GATTCAAGTATCAGAACTAT+AGG + chr7_1:69800121-69800140 None:intergenic 30.0%
TAGTAGACTGTCCAATTTAT+TGG - chr7_1:69800363-69800382 Msa1034330:CDS 30.0%
ATTCGATGTCATAAACAGTT+TGG - chr7_1:69800548-69800567 Msa1034330:intron 30.0%
! TGTCTTTTGTGTATATGACT+TGG - chr7_1:69800617-69800636 Msa1034330:intron 30.0%
! GTCTTTTGTGTATATGACTT+GGG - chr7_1:69800618-69800637 Msa1034330:intron 30.0%
TTGATTACAGCTTGTATGTT+TGG - chr7_1:69800678-69800697 Msa1034330:intron 30.0%
AACGACAATCGAAATCATAA+TGG - chr7_1:69800710-69800729 Msa1034330:intron 30.0%
CATATATACACATACCACTT+TGG + chr7_1:69800806-69800825 None:intergenic 30.0%
AGTGGTATGTGTATATATGT+AGG - chr7_1:69800807-69800826 Msa1034330:intron 30.0%
ATGTGTATATATGTAGGTAC+AGG - chr7_1:69800813-69800832 Msa1034330:intron 30.0%
TGTGTATATATGTAGGTACA+GGG - chr7_1:69800814-69800833 Msa1034330:intron 30.0%
ATTTCTTACTGCTCTCAATT+TGG + chr7_1:69800934-69800953 None:intergenic 30.0%
TAATCTACTTAAGCACACTT+TGG + chr7_1:69801111-69801130 None:intergenic 30.0%
AAAGTGTGCTTAAGTAGATT+AGG - chr7_1:69801110-69801129 Msa1034330:intron 30.0%
CCTTTGTAAGATCGAAATAT+TGG + chr7_1:69801226-69801245 None:intergenic 30.0%
CCAATATTTCGATCTTACAA+AGG - chr7_1:69801223-69801242 Msa1034330:intron 30.0%
TGTAGAAAGAATTATGCATG+AGG - chr7_1:69801283-69801302 Msa1034330:intron 30.0%
AAAGATGGTGACAATTGAAT+GGG - chr7_1:69801363-69801382 Msa1034330:intron 30.0%
GAATTAAACATTCATGTTGC+TGG - chr7_1:69801403-69801422 Msa1034330:intron 30.0%
TTATAGTTTACCAAAATGGC+TGG - chr7_1:69801556-69801575 Msa1034330:intron 30.0%
ACAAAGAATTTCATAGTTGC+CGG - chr7_1:69801610-69801629 Msa1034330:intron 30.0%
! GTTTGACAACTTTTGTCTTT+GGG - chr7_1:69801635-69801654 Msa1034330:intron 30.0%
! TTTGACAACTTTTGTCTTTG+GGG - chr7_1:69801636-69801655 Msa1034330:intron 30.0%
TAACTATTTCCATGCTGAAA+GGG + chr7_1:69801856-69801875 None:intergenic 30.0%
TTAACTATTTCCATGCTGAA+AGG + chr7_1:69801857-69801876 None:intergenic 30.0%
ACGTGAAATACTTAATCCAA+CGG + chr7_1:69801972-69801991 None:intergenic 30.0%
AAAAATGAAGTGCGACAAAT+GGG + chr7_1:69802053-69802072 None:intergenic 30.0%
GTAATATGTAAGACGATGAA+AGG + chr7_1:69802076-69802095 None:intergenic 30.0%
!! AAAAGTATTACATCCAAGGG+TGG + chr7_1:69799859-69799878 None:intergenic 35.0%
! ACCCTTGGATGTAATACTTT+TGG - chr7_1:69799858-69799877 Msa1034330:three_prime_UTR 35.0%
TTACAGGTAGAGTACAGAAA+GGG + chr7_1:69799888-69799907 None:intergenic 35.0%
TTTACAGGTAGAGTACAGAA+AGG + chr7_1:69799889-69799908 None:intergenic 35.0%
! TCTTCTCAGTTTTTGTGTTG+TGG + chr7_1:69799920-69799939 None:intergenic 35.0%
CGTACAAAATAGCGAAAAGA+AGG + chr7_1:69800085-69800104 None:intergenic 35.0%
!! GCTATTTTGTACGTTGAACA+CGG - chr7_1:69800092-69800111 Msa1034330:three_prime_UTR 35.0%
ATAGGGTTTATGCGAAAAGA+GGG + chr7_1:69800251-69800270 None:intergenic 35.0%
AATAGGGTTTATGCGAAAAG+AGG + chr7_1:69800252-69800271 None:intergenic 35.0%
ACTAGGAGCAACCAATAAAT+TGG + chr7_1:69800377-69800396 None:intergenic 35.0%
AACACACAGACTCCTTAAAT+TGG + chr7_1:69800443-69800462 None:intergenic 35.0%
TCAAACCTAATAGACCATAG+AGG + chr7_1:69800475-69800494 None:intergenic 35.0%
CTATTCAATTGTCCTTGATC+CGG - chr7_1:69800517-69800536 Msa1034330:intron 35.0%
TGACATCGAATTCAAAAGAC+CGG + chr7_1:69800539-69800558 None:intergenic 35.0%
!! TGTCATAAACAGTTTGGTGT+TGG - chr7_1:69800554-69800573 Msa1034330:intron 35.0%
! TGGTGTTGGAATTATACTCT+TGG - chr7_1:69800568-69800587 Msa1034330:intron 35.0%
!! TTTGGTTTGGGAAAACTAGT+GGG + chr7_1:69800788-69800807 None:intergenic 35.0%
! TACACATACCACTTTGGTTT+GGG + chr7_1:69800800-69800819 None:intergenic 35.0%
ATACACATACCACTTTGGTT+TGG + chr7_1:69800801-69800820 None:intergenic 35.0%
GTGTATATATGTAGGTACAG+GGG - chr7_1:69800815-69800834 Msa1034330:intron 35.0%
GGAAAAGGAATGCTAAAGAA+AGG - chr7_1:69800888-69800907 Msa1034330:intron 35.0%
GCTAAAGAAAGGATAAGACA+CGG - chr7_1:69800899-69800918 Msa1034330:intron 35.0%
TTACTGCTCTCAATTTGGTT+TGG + chr7_1:69800929-69800948 None:intergenic 35.0%
TCGTATGATTTCAATGAGAG+TGG - chr7_1:69800969-69800988 Msa1034330:intron 35.0%
TTGAGGTGTATTCAGAAAGA+TGG - chr7_1:69801348-69801367 Msa1034330:intron 35.0%
GAAAGATGGTGACAATTGAA+TGG - chr7_1:69801362-69801381 Msa1034330:intron 35.0%
GTGGGAATAATTTGATCCAT+TGG - chr7_1:69801448-69801467 Msa1034330:intron 35.0%
CAAAGAATTTCATAGTTGCC+GGG - chr7_1:69801611-69801630 Msa1034330:intron 35.0%
AAAGAATTTCATAGTTGCCG+GGG - chr7_1:69801612-69801631 Msa1034330:intron 35.0%
! GGTTTGACAACTTTTGTCTT+TGG - chr7_1:69801634-69801653 Msa1034330:intron 35.0%
! TTTGGTTGTACGCAATGTTT+TGG - chr7_1:69801809-69801828 Msa1034330:intron 35.0%
!!! TTGTACGCAATGTTTTGGTT+TGG - chr7_1:69801814-69801833 Msa1034330:intron 35.0%
!!! TGTACGCAATGTTTTGGTTT+GGG - chr7_1:69801815-69801834 Msa1034330:intron 35.0%
AACTATTTCCATGCTGAAAG+GGG + chr7_1:69801855-69801874 None:intergenic 35.0%
CGACAAATGGGGAAAAATAA+TGG + chr7_1:69802041-69802060 None:intergenic 35.0%
AAAATGAAGTGCGACAAATG+GGG + chr7_1:69802052-69802071 None:intergenic 35.0%
GAAAAATGAAGTGCGACAAA+TGG + chr7_1:69802054-69802073 None:intergenic 35.0%
!! AAAAGTATTACATCCAAGGG+TGG + chr7_1:69799859-69799878 None:intergenic 35.0%
! ACCCTTGGATGTAATACTTT+TGG - chr7_1:69799858-69799877 Msa1034330:three_prime_UTR 35.0%
TTACAGGTAGAGTACAGAAA+GGG + chr7_1:69799888-69799907 None:intergenic 35.0%
TTTACAGGTAGAGTACAGAA+AGG + chr7_1:69799889-69799908 None:intergenic 35.0%
! TCTTCTCAGTTTTTGTGTTG+TGG + chr7_1:69799920-69799939 None:intergenic 35.0%
CGTACAAAATAGCGAAAAGA+AGG + chr7_1:69800085-69800104 None:intergenic 35.0%
!! GCTATTTTGTACGTTGAACA+CGG - chr7_1:69800092-69800111 Msa1034330:three_prime_UTR 35.0%
ATAGGGTTTATGCGAAAAGA+GGG + chr7_1:69800251-69800270 None:intergenic 35.0%
AATAGGGTTTATGCGAAAAG+AGG + chr7_1:69800252-69800271 None:intergenic 35.0%
ACTAGGAGCAACCAATAAAT+TGG + chr7_1:69800377-69800396 None:intergenic 35.0%
AACACACAGACTCCTTAAAT+TGG + chr7_1:69800443-69800462 None:intergenic 35.0%
TCAAACCTAATAGACCATAG+AGG + chr7_1:69800475-69800494 None:intergenic 35.0%
CTATTCAATTGTCCTTGATC+CGG - chr7_1:69800517-69800536 Msa1034330:intron 35.0%
TGACATCGAATTCAAAAGAC+CGG + chr7_1:69800539-69800558 None:intergenic 35.0%
!! TGTCATAAACAGTTTGGTGT+TGG - chr7_1:69800554-69800573 Msa1034330:intron 35.0%
! TGGTGTTGGAATTATACTCT+TGG - chr7_1:69800568-69800587 Msa1034330:intron 35.0%
!! TTTGGTTTGGGAAAACTAGT+GGG + chr7_1:69800788-69800807 None:intergenic 35.0%
! TACACATACCACTTTGGTTT+GGG + chr7_1:69800800-69800819 None:intergenic 35.0%
ATACACATACCACTTTGGTT+TGG + chr7_1:69800801-69800820 None:intergenic 35.0%
GTGTATATATGTAGGTACAG+GGG - chr7_1:69800815-69800834 Msa1034330:intron 35.0%
GGAAAAGGAATGCTAAAGAA+AGG - chr7_1:69800888-69800907 Msa1034330:intron 35.0%
GCTAAAGAAAGGATAAGACA+CGG - chr7_1:69800899-69800918 Msa1034330:intron 35.0%
TTACTGCTCTCAATTTGGTT+TGG + chr7_1:69800929-69800948 None:intergenic 35.0%
TCGTATGATTTCAATGAGAG+TGG - chr7_1:69800969-69800988 Msa1034330:intron 35.0%
TTGAGGTGTATTCAGAAAGA+TGG - chr7_1:69801348-69801367 Msa1034330:intron 35.0%
GAAAGATGGTGACAATTGAA+TGG - chr7_1:69801362-69801381 Msa1034330:intron 35.0%
GTGGGAATAATTTGATCCAT+TGG - chr7_1:69801448-69801467 Msa1034330:intron 35.0%
CAAAGAATTTCATAGTTGCC+GGG - chr7_1:69801611-69801630 Msa1034330:intron 35.0%
AAAGAATTTCATAGTTGCCG+GGG - chr7_1:69801612-69801631 Msa1034330:intron 35.0%
! GGTTTGACAACTTTTGTCTT+TGG - chr7_1:69801634-69801653 Msa1034330:intron 35.0%
! TTTGGTTGTACGCAATGTTT+TGG - chr7_1:69801809-69801828 Msa1034330:intron 35.0%
!!! TTGTACGCAATGTTTTGGTT+TGG - chr7_1:69801814-69801833 Msa1034330:intron 35.0%
!!! TGTACGCAATGTTTTGGTTT+GGG - chr7_1:69801815-69801834 Msa1034330:intron 35.0%
AACTATTTCCATGCTGAAAG+GGG + chr7_1:69801855-69801874 None:intergenic 35.0%
CGACAAATGGGGAAAAATAA+TGG + chr7_1:69802041-69802060 None:intergenic 35.0%
AAAATGAAGTGCGACAAATG+GGG + chr7_1:69802052-69802071 None:intergenic 35.0%
GAAAAATGAAGTGCGACAAA+TGG + chr7_1:69802054-69802073 None:intergenic 35.0%
!! CCAAAGGCCTCTGTTTTATT+CGG + chr7_1:69799819-69799838 None:intergenic 40.0%
! TATCTTTTGTTCTCCACCCT+TGG - chr7_1:69799843-69799862 Msa1034330:three_prime_UTR 40.0%
! TCTCAGTTTTTGTGTTGTGG+CGG + chr7_1:69799917-69799936 None:intergenic 40.0%
AGAAGAGAAAGAGAAAGCGA+CGG + chr7_1:69800043-69800062 None:intergenic 40.0%
TAGGGTTTATGCGAAAAGAG+GGG + chr7_1:69800250-69800269 None:intergenic 40.0%
TCAACCACAGACAACAAACT+AGG + chr7_1:69800394-69800413 None:intergenic 40.0%
GTGTTAGAATCAGACCTCTA+TGG - chr7_1:69800458-69800477 Msa1034330:intron 40.0%
TCAGACCTCTATGGTCTATT+AGG - chr7_1:69800467-69800486 Msa1034330:intron 40.0%
CGGATCAAGGACAATTGAAT+AGG + chr7_1:69800519-69800538 None:intergenic 40.0%
GAATTCAAAAGACCGGATCA+AGG + chr7_1:69800532-69800551 None:intergenic 40.0%
!! TTGGTTTGGGAAAACTAGTG+GGG + chr7_1:69800787-69800806 None:intergenic 40.0%
!! CTTTGGTTTGGGAAAACTAG+TGG + chr7_1:69800789-69800808 None:intergenic 40.0%
!! CTAGTTTTCCCAAACCAAAG+TGG - chr7_1:69800789-69800808 Msa1034330:intron 40.0%
AAACAAGAGGCTAAGATGTG+AGG - chr7_1:69800867-69800886 Msa1034330:intron 40.0%
AGATTAGGAAAGCAACTGTG+AGG - chr7_1:69801125-69801144 Msa1034330:intron 40.0%
! ACCAAAATGGCTGGAGTATT+AGG - chr7_1:69801565-69801584 Msa1034330:intron 40.0%
! TCCTAATACTCCAGCCATTT+TGG + chr7_1:69801569-69801588 None:intergenic 40.0%
! GCTGGAGTATTAGGATATAG+TGG - chr7_1:69801574-69801593 Msa1034330:intron 40.0%
AGTATTAGGATATAGTGGCC+TGG - chr7_1:69801579-69801598 Msa1034330:intron 40.0%
AAGAATTTCATAGTTGCCGG+GGG - chr7_1:69801613-69801632 Msa1034330:intron 40.0%
! AATGCTTGATTCGCCTTCTT+TGG + chr7_1:69801743-69801762 None:intergenic 40.0%
CGAATCAAGCATTCCATCAA+AGG - chr7_1:69801750-69801769 Msa1034330:intron 40.0%
ACTGTAAGCAACTAAGCTCT+AGG + chr7_1:69801776-69801795 None:intergenic 40.0%
!! CCAAAGGCCTCTGTTTTATT+CGG + chr7_1:69799819-69799838 None:intergenic 40.0%
! TATCTTTTGTTCTCCACCCT+TGG - chr7_1:69799843-69799862 Msa1034330:three_prime_UTR 40.0%
! TCTCAGTTTTTGTGTTGTGG+CGG + chr7_1:69799917-69799936 None:intergenic 40.0%
AGAAGAGAAAGAGAAAGCGA+CGG + chr7_1:69800043-69800062 None:intergenic 40.0%
TAGGGTTTATGCGAAAAGAG+GGG + chr7_1:69800250-69800269 None:intergenic 40.0%
TCAACCACAGACAACAAACT+AGG + chr7_1:69800394-69800413 None:intergenic 40.0%
GTGTTAGAATCAGACCTCTA+TGG - chr7_1:69800458-69800477 Msa1034330:intron 40.0%
TCAGACCTCTATGGTCTATT+AGG - chr7_1:69800467-69800486 Msa1034330:intron 40.0%
CGGATCAAGGACAATTGAAT+AGG + chr7_1:69800519-69800538 None:intergenic 40.0%
GAATTCAAAAGACCGGATCA+AGG + chr7_1:69800532-69800551 None:intergenic 40.0%
!! TTGGTTTGGGAAAACTAGTG+GGG + chr7_1:69800787-69800806 None:intergenic 40.0%
!! CTTTGGTTTGGGAAAACTAG+TGG + chr7_1:69800789-69800808 None:intergenic 40.0%
!! CTAGTTTTCCCAAACCAAAG+TGG - chr7_1:69800789-69800808 Msa1034330:intron 40.0%
AAACAAGAGGCTAAGATGTG+AGG - chr7_1:69800867-69800886 Msa1034330:intron 40.0%
AGATTAGGAAAGCAACTGTG+AGG - chr7_1:69801125-69801144 Msa1034330:intron 40.0%
! ACCAAAATGGCTGGAGTATT+AGG - chr7_1:69801565-69801584 Msa1034330:intron 40.0%
! TCCTAATACTCCAGCCATTT+TGG + chr7_1:69801569-69801588 None:intergenic 40.0%
! GCTGGAGTATTAGGATATAG+TGG - chr7_1:69801574-69801593 Msa1034330:intron 40.0%
AGTATTAGGATATAGTGGCC+TGG - chr7_1:69801579-69801598 Msa1034330:intron 40.0%
AAGAATTTCATAGTTGCCGG+GGG - chr7_1:69801613-69801632 Msa1034330:intron 40.0%
! AATGCTTGATTCGCCTTCTT+TGG + chr7_1:69801743-69801762 None:intergenic 40.0%
CGAATCAAGCATTCCATCAA+AGG - chr7_1:69801750-69801769 Msa1034330:intron 40.0%
ACTGTAAGCAACTAAGCTCT+AGG + chr7_1:69801776-69801795 None:intergenic 40.0%
CCGAATAAAACAGAGGCCTT+TGG - chr7_1:69799816-69799835 Msa1034330:three_prime_UTR 45.0%
TGTGAAATGAAGTGGCAGCT+TGG + chr7_1:69799966-69799985 None:intergenic 45.0%
! GTCGCTTTCTCTTTCTCTTC+TGG - chr7_1:69800042-69800061 Msa1034330:three_prime_UTR 45.0%
!! TGCTCCTAGTTTGTTGTCTG+TGG - chr7_1:69800387-69800406 Msa1034330:intron 45.0%
GAGGCTAAGATGTGAGGAAA+AGG - chr7_1:69800873-69800892 Msa1034330:intron 45.0%
AATTCTTTGTCTTAGGCGCC+AGG + chr7_1:69801600-69801619 None:intergenic 45.0%
GACAAAAGTTGTCAAACCCC+CGG + chr7_1:69801632-69801651 None:intergenic 45.0%
TTCTACACCATGAGAGCAGT+TGG - chr7_1:69801664-69801683 Msa1034330:intron 45.0%
!! TGGAGGTACTGATGAACTAC+AGG - chr7_1:69801684-69801703 Msa1034330:intron 45.0%
!! ACTAAGCTCTAGGCCTTTGA+TGG + chr7_1:69801766-69801785 None:intergenic 45.0%
! GCTTTTAACCCCTTTCAGCA+TGG - chr7_1:69801844-69801863 Msa1034330:intron 45.0%
ACGTGAGACTCAACATCTCT+TGG - chr7_1:69801988-69802007 Msa1034330:intron 45.0%
CCGAATAAAACAGAGGCCTT+TGG - chr7_1:69799816-69799835 Msa1034330:three_prime_UTR 45.0%
TGTGAAATGAAGTGGCAGCT+TGG + chr7_1:69799966-69799985 None:intergenic 45.0%
! GTCGCTTTCTCTTTCTCTTC+TGG - chr7_1:69800042-69800061 Msa1034330:three_prime_UTR 45.0%
!! TGCTCCTAGTTTGTTGTCTG+TGG - chr7_1:69800387-69800406 Msa1034330:intron 45.0%
GAGGCTAAGATGTGAGGAAA+AGG - chr7_1:69800873-69800892 Msa1034330:intron 45.0%
AATTCTTTGTCTTAGGCGCC+AGG + chr7_1:69801600-69801619 None:intergenic 45.0%
GACAAAAGTTGTCAAACCCC+CGG + chr7_1:69801632-69801651 None:intergenic 45.0%
TTCTACACCATGAGAGCAGT+TGG - chr7_1:69801664-69801683 Msa1034330:intron 45.0%
!! TGGAGGTACTGATGAACTAC+AGG - chr7_1:69801684-69801703 Msa1034330:intron 45.0%
!! ACTAAGCTCTAGGCCTTTGA+TGG + chr7_1:69801766-69801785 None:intergenic 45.0%
! GCTTTTAACCCCTTTCAGCA+TGG - chr7_1:69801844-69801863 Msa1034330:intron 45.0%
ACGTGAGACTCAACATCTCT+TGG - chr7_1:69801988-69802007 Msa1034330:intron 45.0%
GTAGGTACAGGGGTAGTTCA+AGG - chr7_1:69800825-69800844 Msa1034330:intron 50.0%
TACACCATGAGAGCAGTTGG+AGG - chr7_1:69801667-69801686 Msa1034330:intron 50.0%
AGTACCTCCAACTGCTCTCA+TGG + chr7_1:69801674-69801693 None:intergenic 50.0%
!! GCCTTCTTTGGTGCTCTTGT+CGG + chr7_1:69801731-69801750 None:intergenic 50.0%
GTAGGTACAGGGGTAGTTCA+AGG - chr7_1:69800825-69800844 Msa1034330:intron 50.0%
TACACCATGAGAGCAGTTGG+AGG - chr7_1:69801667-69801686 Msa1034330:intron 50.0%
AGTACCTCCAACTGCTCTCA+TGG + chr7_1:69801674-69801693 None:intergenic 50.0%
!! GCCTTCTTTGGTGCTCTTGT+CGG + chr7_1:69801731-69801750 None:intergenic 50.0%
!! GTTGTGGCGGTGCTGTTTAC+AGG + chr7_1:69799904-69799923 None:intergenic 55.0%
TCACTAAAACGCCGCCGTAG+AGG - chr7_1:69799992-69800011 Msa1034330:three_prime_UTR 55.0%
AGAAAGAGAAAGCGACGGCG+AGG + chr7_1:69800038-69800057 None:intergenic 55.0%
!! GCCGACAAGAGCACCAAAGA+AGG - chr7_1:69801727-69801746 Msa1034330:intron 55.0%
!! GTTGTGGCGGTGCTGTTTAC+AGG + chr7_1:69799904-69799923 None:intergenic 55.0%
TCACTAAAACGCCGCCGTAG+AGG - chr7_1:69799992-69800011 Msa1034330:three_prime_UTR 55.0%
AGAAAGAGAAAGCGACGGCG+AGG + chr7_1:69800038-69800057 None:intergenic 55.0%
!! GCCGACAAGAGCACCAAAGA+AGG - chr7_1:69801727-69801746 Msa1034330:intron 55.0%
AAGAGAAAGCGACGGCGAGG+TGG + chr7_1:69800035-69800054 None:intergenic 60.0%
AAGAGAAAGCGACGGCGAGG+TGG + chr7_1:69800035-69800054 None:intergenic 60.0%
CGGTCTTGGGTCCTCTACGG+CGG + chr7_1:69800006-69800025 None:intergenic 65.0%
CGGCGGTCTTGGGTCCTCTA+CGG + chr7_1:69800009-69800028 None:intergenic 65.0%
CGGTCTTGGGTCCTCTACGG+CGG + chr7_1:69800006-69800025 None:intergenic 65.0%
CGGCGGTCTTGGGTCCTCTA+CGG + chr7_1:69800009-69800028 None:intergenic 65.0%
GAGGTGGACGCGGCGGTCTT+GGG + chr7_1:69800019-69800038 None:intergenic 70.0%
AAGCGACGGCGAGGTGGACG+CGG + chr7_1:69800029-69800048 None:intergenic 70.0%
GAGGTGGACGCGGCGGTCTT+GGG + chr7_1:69800019-69800038 None:intergenic 70.0%
AAGCGACGGCGAGGTGGACG+CGG + chr7_1:69800029-69800048 None:intergenic 70.0%
CGAGGTGGACGCGGCGGTCT+TGG + chr7_1:69800020-69800039 None:intergenic 75.0%
CGAGGTGGACGCGGCGGTCT+TGG + chr7_1:69800020-69800039 None:intergenic 75.0%
! CGACGGCGAGGTGGACGCGG+CGG + chr7_1:69800026-69800045 None:intergenic 80.0%
! CGACGGCGAGGTGGACGCGG+CGG + chr7_1:69800026-69800045 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 69799788 69802176 69799788 ID=Msa1034330;Name=Msa1034330
chr7_1 mRNA 69799788 69802176 69799788 ID=Msa1034330-mRNA-1;Parent=Msa1034330;Name=Msa1034330-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.5|0.33|0.66|0|0.33|3|399|105
chr7_1 exon 69799788 69800402 69799788 ID=Msa1034330-mRNA-1:exon:16546;Parent=Msa1034330-mRNA-1
chr7_1 exon 69801901 69801996 69801901 ID=Msa1034330-mRNA-1:exon:16545;Parent=Msa1034330-mRNA-1
chr7_1 exon 69802171 69802176 69802171 ID=Msa1034330-mRNA-1:exon:16544;Parent=Msa1034330-mRNA-1
chr7_1 CDS 69802171 69802176 69802171 ID=Msa1034330-mRNA-1:cds;Parent=Msa1034330-mRNA-1
chr7_1 CDS 69801901 69801996 69801901 ID=Msa1034330-mRNA-1:cds;Parent=Msa1034330-mRNA-1
chr7_1 CDS 69800187 69800402 69800187 ID=Msa1034330-mRNA-1:cds;Parent=Msa1034330-mRNA-1
chr7_1 three_prime_UTR 69799788 69800186 69799788 ID=Msa1034330-mRNA-1:three_prime_utr;Parent=Msa1034330-mRNA-1
Gene Sequence

>Msa1034330

GTCATTCTGCCACTTCATTTCACAAAAAACTCACTAAAACGCCGCCGTAGAGGACCCAAGACCGCCGCGTCCACCTCGCCGTCGCTTTCTCTTTCTCTTCTGTTTACCAAAATGGCTGGAGTATTAGGATATAGTGGCCTGGCGCCTAAGACAAAGAATTTCATAGTTGCCGGGGGTTTGACAACTTTTGTCTTTGGGGCATACTTCTACACCATGAGAGCAGTTGGAGGTACTGATGAACTACAGGTAGCAATAGATAAGTTTGAAGCCGACAAGAGCACCAAAGAAGGCGAATCAAGCATTCCATCAAAGGCCTAG
Protein sequence

>Msa1034330

VILPLHFTKNSLKRRRRGPKTAASTSPSLSLSLLFTKMAGVLGYSGLAPKTKNFIVAGGLTTFVFGAYFYTMRAVGGTDELQVAIDKFEADKSTKEGESSIPSKA*