AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1159670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1159670 MtrunA17_Chr7g0257181 100.000 68 0 0 1 68 1 68 2.00e-44 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1159670 AT2G43780.3 68.657 67 20 1 1 67 1 66 6.15e-26 90.5
Msa1159670 AT2G43780.1 68.657 67 20 1 1 67 1 66 6.15e-26 90.5
Msa1159670 AT2G43780.2 68.657 67 20 1 1 67 1 66 6.15e-26 90.5
Msa1159670 AT2G43780.4 67.647 68 21 1 1 68 27 93 7.61e-26 90.9

Find 54 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCTTGGATGTAATAATTT+TGG 0.087490 7_4:-49325864 Msa1159670:five_prime_UTR
TCCTAATACTCCAGCCATTT+TGG 0.197201 7_4:+49324126 None:intergenic
CGAGGTGGACGCGGCGGTCT+TGG 0.201704 7_4:+49325714 None:intergenic
TTCTTTATAGTTTACCAAAA+TGG 0.227595 7_4:-49324140 Msa1159670:intron
GTCGCTTTCTCTTTCTCTTC+TGG 0.248537 7_4:-49325689 Msa1159670:intron
AATGCTTGATTCGCCTTCTT+TGG 0.262054 7_4:+49323952 None:intergenic
CCTTGTATTTAATAAAGTTT+AGG 0.300367 7_4:-49323801 Msa1159670:three_prime_UTR
ACTATGAAATTCTTTGTCTT+AGG 0.312137 7_4:+49324088 None:intergenic
ACCAAAATGGCTGGAGTATT+AGG 0.318096 7_4:-49324127 Msa1159670:exon
GCCTTCTTTGGTGCTCTTGT+CGG 0.348229 7_4:+49323964 None:intergenic
AACGGTTATTATGTTTCAAT+TGG 0.349565 7_4:+49323741 None:intergenic
GAGGTGGACGCGGCGGTCTT+GGG 0.355664 7_4:+49325715 None:intergenic
TTTGGTTGTACGCAATGTTT+TGG 0.369171 7_4:-49323883 Msa1159670:three_prime_UTR
ACAAAGAATTTCATAGTTGC+CGG 0.374694 7_4:-49324082 Msa1159670:CDS
ACTAAGCTCTAGGCCTTTGA+TGG 0.419130 7_4:+49323929 None:intergenic
CCTAAACTTTATTAAATACA+AGG 0.423444 7_4:+49323801 None:intergenic
AATAACATGAACAGTACTTA+AGG 0.452950 7_4:+49323766 None:intergenic
TTATAGTTTACCAAAATGGC+TGG 0.453643 7_4:-49324136 Msa1159670:intron
ACAGTACACAAATAATACTT+TGG 0.456449 7_4:-49323901 Msa1159670:three_prime_UTR
TAACTATTTCCATGCTGAAA+GGG 0.462522 7_4:+49323839 None:intergenic
ACGTGAGACTCAATATCTCT+TGG 0.468646 7_4:-49323704 Msa1159670:three_prime_UTR
AAGCGACGGCGAGGTGGACG+CGG 0.486196 7_4:+49325705 None:intergenic
CAAAGAATTTCATAGTTGCC+GGG 0.488180 7_4:-49324081 Msa1159670:CDS
CGGTGCTGTTTACAAGAGAA+AGG 0.489555 7_4:+49325836 None:intergenic
AATTCTTTGTCTTAGGCGCC+AGG 0.505757 7_4:+49324095 None:intergenic
AGTACCTCCAACTGCTCTCA+TGG 0.506868 7_4:+49324021 None:intergenic
GCTGGAGTATTAGGATATAG+TGG 0.507617 7_4:-49324118 Msa1159670:CDS
TTAACTATTTCCATGCTGAA+AGG 0.507702 7_4:+49323838 None:intergenic
CGAATCAAGCATTCCATCAA+AGG 0.507874 7_4:-49323942 Msa1159670:CDS
ATCCAAAATTATTACATCCA+AGG 0.508289 7_4:+49325862 None:intergenic
GCCGACAAGAGCACCAAAGA+AGG 0.517286 7_4:-49323965 Msa1159670:CDS
TTCTACACCATGAGAGCAGT+TGG 0.517929 7_4:-49324028 Msa1159670:CDS
AATTGAAACATAATAACCGT+TGG 0.524572 7_4:-49323739 Msa1159670:three_prime_UTR
TGGAGGTACTGATGAACTAC+AGG 0.526085 7_4:-49324008 Msa1159670:CDS
TCCAAAATTATTACATCCAA+GGG 0.533453 7_4:+49325863 None:intergenic
GTAATATGTAAGACGATGAA+AGG 0.564304 7_4:+49323619 None:intergenic
AACTATTTCCATGCTGAAAG+GGG 0.565638 7_4:+49323840 None:intergenic
TTACTAAAACGCCGCCGTAG+AGG 0.566475 7_4:-49325739 Msa1159670:five_prime_UTR
ACTGTAAGCAACTAAGCTCT+AGG 0.568639 7_4:+49323919 None:intergenic
CGGCGGTCTTGGGTCCTCTA+CGG 0.574685 7_4:+49325725 None:intergenic
GACAAAAGTTGTCAAACCCC+CGG 0.577777 7_4:+49324063 None:intergenic
GGTGCTGTTTACAAGAGAAA+GGG 0.590121 7_4:+49325837 None:intergenic
AAGAGAAAGCGACGGCGAGG+TGG 0.591651 7_4:+49325699 None:intergenic
TTGTGAAATGAGTGGCAGCT+TGG 0.593040 7_4:+49325767 None:intergenic
AGAAAGAGAAAGCGACGGCG+AGG 0.608606 7_4:+49325696 None:intergenic
TACACCATGAGAGCAGTTGG+AGG 0.610981 7_4:-49324025 Msa1159670:CDS
AGTATTAGGATATAGTGGCC+TGG 0.611243 7_4:-49324113 Msa1159670:CDS
AAAGAATTTCATAGTTGCCG+GGG 0.615205 7_4:-49324080 Msa1159670:CDS
AGAAGAGAAAGAGAAAGCGA+CGG 0.618005 7_4:+49325691 None:intergenic
ACGTGAAATACTTAATCCAA+CGG 0.624324 7_4:+49323723 None:intergenic
CGACGGCGAGGTGGACGCGG+CGG 0.634918 7_4:+49325708 None:intergenic
AAGAATTTCATAGTTGCCGG+GGG 0.655049 7_4:-49324079 Msa1159670:CDS
AAAATGAAGTGCGACAAATG+GGG 0.685319 7_4:+49323643 None:intergenic
CGGTCTTGGGTCCTCTACGG+CGG 0.699173 7_4:+49325728 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAAAAGAAAAAAAAAATA+GGG + chr7_4:49323936-49323955 None:intergenic 10.0%
!! TTTGAAAAGAAAAAAAAAAT+AGG + chr7_4:49323937-49323956 None:intergenic 10.0%
!!! TTTTTTTTCTTTTCAAATTT+CGG - chr7_4:49323940-49323959 Msa1159670:CDS 10.0%
!! TTGAAAAGAAAAAAAAAATA+GGG + chr7_4:49323936-49323955 None:intergenic 10.0%
!! TTTGAAAAGAAAAAAAAAAT+AGG + chr7_4:49323937-49323956 None:intergenic 10.0%
!!! TTTTTTTTCTTTTCAAATTT+CGG - chr7_4:49323940-49323959 Msa1159670:CDS 10.0%
!!! TTCTACAATTTCTAAAAAAA+GGG + chr7_4:49324977-49324996 None:intergenic 15.0%
!!! TTTCTACAATTTCTAAAAAA+AGG + chr7_4:49324978-49324997 None:intergenic 15.0%
!! TAGATTGTGTTAAAAAAATT+GGG - chr7_4:49325855-49325874 Msa1159670:five_prime_UTR 15.0%
!!! TTCTACAATTTCTAAAAAAA+GGG + chr7_4:49324977-49324996 None:intergenic 15.0%
!!! TTTCTACAATTTCTAAAAAA+AGG + chr7_4:49324978-49324997 None:intergenic 15.0%
!! TAGATTGTGTTAAAAAAATT+GGG - chr7_4:49325855-49325874 Msa1159670:five_prime_UTR 15.0%
!! TTTGCTAATATTCCAATTTA+AGG - chr7_4:49324098-49324117 Msa1159670:CDS 20.0%
!! TGTAAATCATAAAAGTTGTT+AGG - chr7_4:49324447-49324466 Msa1159670:intron 20.0%
!! AACTTAGTTTAAAAAACAAG+AGG - chr7_4:49324561-49324580 Msa1159670:intron 20.0%
!!! GAGATTTTAATTGTATTATG+TGG - chr7_4:49324721-49324740 Msa1159670:intron 20.0%
!! TATATCTACAAGATTAGATT+AGG - chr7_4:49324860-49324879 Msa1159670:intron 20.0%
!! AAATAGTTAAAATACTGCAT+TGG + chr7_4:49325027-49325046 None:intergenic 20.0%
!!! ATGCAGTATTTTAACTATTT+AGG - chr7_4:49325027-49325046 Msa1159670:intron 20.0%
!!! TGATTTGTAATCTTTTTACA+AGG - chr7_4:49325133-49325152 Msa1159670:intron 20.0%
!! TGATATATATTACAATCCAA+TGG + chr7_4:49325174-49325193 None:intergenic 20.0%
!! TTCTTTATAGTTTACCAAAA+TGG - chr7_4:49325259-49325278 Msa1159670:intron 20.0%
!! CCTAAACTTTATTAAATACA+AGG + chr7_4:49325601-49325620 None:intergenic 20.0%
!! CCTTGTATTTAATAAAGTTT+AGG - chr7_4:49325598-49325617 Msa1159670:intron 20.0%
!! CTAGATTGTGTTAAAAAAAT+TGG - chr7_4:49325854-49325873 Msa1159670:five_prime_UTR 20.0%
!! TTTGCTAATATTCCAATTTA+AGG - chr7_4:49324098-49324117 Msa1159670:CDS 20.0%
!! TGTAAATCATAAAAGTTGTT+AGG - chr7_4:49324447-49324466 Msa1159670:intron 20.0%
!! AACTTAGTTTAAAAAACAAG+AGG - chr7_4:49324561-49324580 Msa1159670:intron 20.0%
!!! GAGATTTTAATTGTATTATG+TGG - chr7_4:49324721-49324740 Msa1159670:intron 20.0%
!! TATATCTACAAGATTAGATT+AGG - chr7_4:49324860-49324879 Msa1159670:intron 20.0%
!! AAATAGTTAAAATACTGCAT+TGG + chr7_4:49325027-49325046 None:intergenic 20.0%
!!! ATGCAGTATTTTAACTATTT+AGG - chr7_4:49325027-49325046 Msa1159670:intron 20.0%
!!! TGATTTGTAATCTTTTTACA+AGG - chr7_4:49325133-49325152 Msa1159670:intron 20.0%
!! TGATATATATTACAATCCAA+TGG + chr7_4:49325174-49325193 None:intergenic 20.0%
!! TTCTTTATAGTTTACCAAAA+TGG - chr7_4:49325259-49325278 Msa1159670:intron 20.0%
!! CCTAAACTTTATTAAATACA+AGG + chr7_4:49325601-49325620 None:intergenic 20.0%
!! CCTTGTATTTAATAAAGTTT+AGG - chr7_4:49325598-49325617 Msa1159670:intron 20.0%
!! CTAGATTGTGTTAAAAAAAT+TGG - chr7_4:49325854-49325873 Msa1159670:five_prime_UTR 20.0%
!!! TAAGTTTTTTGTGAAATGAG+TGG + chr7_4:49323643-49323662 None:intergenic 25.0%
! ATTCAAGTATCAGAACTATA+GGG + chr7_4:49323788-49323807 None:intergenic 25.0%
!! GAATGTTTTTCTTTCAATCT+AGG - chr7_4:49323810-49323829 Msa1159670:three_prime_UTR 25.0%
!! TTTTCTTTCAATCTAGGAAA+TGG - chr7_4:49323816-49323835 Msa1159670:three_prime_UTR 25.0%
! TGATTGTTAGAATCTCTAAT+TGG - chr7_4:49323976-49323995 Msa1159670:CDS 25.0%
! TAGTAGACTGTTCAATTTAT+TGG - chr7_4:49324033-49324052 Msa1159670:CDS 25.0%
! TTGAATAGGAATATGATTTC+TGG + chr7_4:49324175-49324194 None:intergenic 25.0%
! AAGCTTAAAAGATTGCAAAA+TGG + chr7_4:49324322-49324341 None:intergenic 25.0%
! ATCATAAAAGTTGTTAGGTA+AGG - chr7_4:49324452-49324471 Msa1159670:intron 25.0%
! CAATATTTCGATCTTACAAA+GGG - chr7_4:49324931-49324950 Msa1159670:intron 25.0%
!! TAACTATTTAGGACTATTTG+AGG - chr7_4:49325038-49325057 Msa1159670:intron 25.0%
!! TTTGTAATCTTTTTACAAGG+TGG - chr7_4:49325136-49325155 Msa1159670:intron 25.0%
!! TTGTAATCTTTTTACAAGGT+GGG - chr7_4:49325137-49325156 Msa1159670:intron 25.0%
! ACTATGAAATTCTTTGTCTT+AGG + chr7_4:49325314-49325333 None:intergenic 25.0%
! ACAGTACACAAATAATACTT+TGG - chr7_4:49325498-49325517 Msa1159670:intron 25.0%
! AATAACATGAACAGTACTTA+AGG + chr7_4:49325636-49325655 None:intergenic 25.0%
! AACGGTTATTATGTTTCAAT+TGG + chr7_4:49325661-49325680 None:intergenic 25.0%
! AATTGAAACATAATAACCGT+TGG - chr7_4:49325660-49325679 Msa1159670:intron 25.0%
!!! TAAGTTTTTTGTGAAATGAG+TGG + chr7_4:49323643-49323662 None:intergenic 25.0%
! ATTCAAGTATCAGAACTATA+GGG + chr7_4:49323788-49323807 None:intergenic 25.0%
!! GAATGTTTTTCTTTCAATCT+AGG - chr7_4:49323810-49323829 Msa1159670:three_prime_UTR 25.0%
!! TTTTCTTTCAATCTAGGAAA+TGG - chr7_4:49323816-49323835 Msa1159670:three_prime_UTR 25.0%
! TGATTGTTAGAATCTCTAAT+TGG - chr7_4:49323976-49323995 Msa1159670:CDS 25.0%
! TAGTAGACTGTTCAATTTAT+TGG - chr7_4:49324033-49324052 Msa1159670:CDS 25.0%
! TTGAATAGGAATATGATTTC+TGG + chr7_4:49324175-49324194 None:intergenic 25.0%
! AAGCTTAAAAGATTGCAAAA+TGG + chr7_4:49324322-49324341 None:intergenic 25.0%
! ATCATAAAAGTTGTTAGGTA+AGG - chr7_4:49324452-49324471 Msa1159670:intron 25.0%
! CAATATTTCGATCTTACAAA+GGG - chr7_4:49324931-49324950 Msa1159670:intron 25.0%
!! TAACTATTTAGGACTATTTG+AGG - chr7_4:49325038-49325057 Msa1159670:intron 25.0%
!! TTTGTAATCTTTTTACAAGG+TGG - chr7_4:49325136-49325155 Msa1159670:intron 25.0%
!! TTGTAATCTTTTTACAAGGT+GGG - chr7_4:49325137-49325156 Msa1159670:intron 25.0%
! ACTATGAAATTCTTTGTCTT+AGG + chr7_4:49325314-49325333 None:intergenic 25.0%
! ACAGTACACAAATAATACTT+TGG - chr7_4:49325498-49325517 Msa1159670:intron 25.0%
! AATAACATGAACAGTACTTA+AGG + chr7_4:49325636-49325655 None:intergenic 25.0%
! AACGGTTATTATGTTTCAAT+TGG + chr7_4:49325661-49325680 None:intergenic 25.0%
! AATTGAAACATAATAACCGT+TGG - chr7_4:49325660-49325679 Msa1159670:intron 25.0%
GTACAAAATAGCGAAAAGAA+GGG + chr7_4:49323752-49323771 None:intergenic 30.0%
GATTCAAGTATCAGAACTAT+AGG + chr7_4:49323789-49323808 None:intergenic 30.0%
ATTCGATGTCATAAACAGTT+TGG - chr7_4:49324218-49324237 Msa1159670:intron 30.0%
! TGTCTTTTGTGTATATGACT+TGG - chr7_4:49324287-49324306 Msa1159670:intron 30.0%
TTGATTACAGCTTGTATGTT+TGG - chr7_4:49324347-49324366 Msa1159670:intron 30.0%
! GATTGTCGTTTAAAGCTTAA+GGG + chr7_4:49324407-49324426 None:intergenic 30.0%
AACGACAATCGAAATCATAA+TGG - chr7_4:49324417-49324436 Msa1159670:intron 30.0%
CATATATACACATACCACTT+TGG + chr7_4:49324513-49324532 None:intergenic 30.0%
AGTGGTATGTGTATATATGT+AGG - chr7_4:49324514-49324533 Msa1159670:intron 30.0%
ATGTGTATATATGTAGGTAC+AGG - chr7_4:49324520-49324539 Msa1159670:intron 30.0%
TGTGTATATATGTAGGTACA+GGG - chr7_4:49324521-49324540 Msa1159670:intron 30.0%
GCTAAAGAAAGGATAAGATA+CGG - chr7_4:49324606-49324625 Msa1159670:intron 30.0%
ATTTCTTACTGCTCTCAATT+TGG + chr7_4:49324641-49324660 None:intergenic 30.0%
TAATCTACTTAAGCACACTT+TGG + chr7_4:49324818-49324837 None:intergenic 30.0%
AAAGTGTGCTTAAGTAGATT+AGG - chr7_4:49324817-49324836 Msa1159670:intron 30.0%
CCTTTGTAAGATCGAAATAT+TGG + chr7_4:49324933-49324952 None:intergenic 30.0%
CCAATATTTCGATCTTACAA+AGG - chr7_4:49324930-49324949 Msa1159670:intron 30.0%
TGTAGAAAGAATTATGCATG+AGG - chr7_4:49324990-49325009 Msa1159670:intron 30.0%
AAAGATGGTGACAATTGAAT+GGG - chr7_4:49325070-49325089 Msa1159670:intron 30.0%
GAATTAAACATTCATGTTGC+TGG - chr7_4:49325110-49325129 Msa1159670:intron 30.0%
TTATAGTTTACCAAAATGGC+TGG - chr7_4:49325263-49325282 Msa1159670:intron 30.0%
ACAAAGAATTTCATAGTTGC+CGG - chr7_4:49325317-49325336 Msa1159670:intron 30.0%
! GTTTGACAACTTTTGTCTTT+GGG - chr7_4:49325342-49325361 Msa1159670:intron 30.0%
! TTTGACAACTTTTGTCTTTG+GGG - chr7_4:49325343-49325362 Msa1159670:intron 30.0%
TAACTATTTCCATGCTGAAA+GGG + chr7_4:49325563-49325582 None:intergenic 30.0%
TTAACTATTTCCATGCTGAA+AGG + chr7_4:49325564-49325583 None:intergenic 30.0%
ACGTGAAATACTTAATCCAA+CGG + chr7_4:49325679-49325698 None:intergenic 30.0%
AAAAATGAAGTGCGACAAAT+GGG + chr7_4:49325760-49325779 None:intergenic 30.0%
GTAATATGTAAGACGATGAA+AGG + chr7_4:49325783-49325802 None:intergenic 30.0%
GTACAAAATAGCGAAAAGAA+GGG + chr7_4:49323752-49323771 None:intergenic 30.0%
GATTCAAGTATCAGAACTAT+AGG + chr7_4:49323789-49323808 None:intergenic 30.0%
ATTCGATGTCATAAACAGTT+TGG - chr7_4:49324218-49324237 Msa1159670:intron 30.0%
! TGTCTTTTGTGTATATGACT+TGG - chr7_4:49324287-49324306 Msa1159670:intron 30.0%
TTGATTACAGCTTGTATGTT+TGG - chr7_4:49324347-49324366 Msa1159670:intron 30.0%
! GATTGTCGTTTAAAGCTTAA+GGG + chr7_4:49324407-49324426 None:intergenic 30.0%
AACGACAATCGAAATCATAA+TGG - chr7_4:49324417-49324436 Msa1159670:intron 30.0%
CATATATACACATACCACTT+TGG + chr7_4:49324513-49324532 None:intergenic 30.0%
AGTGGTATGTGTATATATGT+AGG - chr7_4:49324514-49324533 Msa1159670:intron 30.0%
ATGTGTATATATGTAGGTAC+AGG - chr7_4:49324520-49324539 Msa1159670:intron 30.0%
TGTGTATATATGTAGGTACA+GGG - chr7_4:49324521-49324540 Msa1159670:intron 30.0%
GCTAAAGAAAGGATAAGATA+CGG - chr7_4:49324606-49324625 Msa1159670:intron 30.0%
ATTTCTTACTGCTCTCAATT+TGG + chr7_4:49324641-49324660 None:intergenic 30.0%
TAATCTACTTAAGCACACTT+TGG + chr7_4:49324818-49324837 None:intergenic 30.0%
AAAGTGTGCTTAAGTAGATT+AGG - chr7_4:49324817-49324836 Msa1159670:intron 30.0%
CCTTTGTAAGATCGAAATAT+TGG + chr7_4:49324933-49324952 None:intergenic 30.0%
CCAATATTTCGATCTTACAA+AGG - chr7_4:49324930-49324949 Msa1159670:intron 30.0%
TGTAGAAAGAATTATGCATG+AGG - chr7_4:49324990-49325009 Msa1159670:intron 30.0%
AAAGATGGTGACAATTGAAT+GGG - chr7_4:49325070-49325089 Msa1159670:intron 30.0%
GAATTAAACATTCATGTTGC+TGG - chr7_4:49325110-49325129 Msa1159670:intron 30.0%
TTATAGTTTACCAAAATGGC+TGG - chr7_4:49325263-49325282 Msa1159670:intron 30.0%
ACAAAGAATTTCATAGTTGC+CGG - chr7_4:49325317-49325336 Msa1159670:intron 30.0%
! GTTTGACAACTTTTGTCTTT+GGG - chr7_4:49325342-49325361 Msa1159670:intron 30.0%
! TTTGACAACTTTTGTCTTTG+GGG - chr7_4:49325343-49325362 Msa1159670:intron 30.0%
TAACTATTTCCATGCTGAAA+GGG + chr7_4:49325563-49325582 None:intergenic 30.0%
TTAACTATTTCCATGCTGAA+AGG + chr7_4:49325564-49325583 None:intergenic 30.0%
ACGTGAAATACTTAATCCAA+CGG + chr7_4:49325679-49325698 None:intergenic 30.0%
AAAAATGAAGTGCGACAAAT+GGG + chr7_4:49325760-49325779 None:intergenic 30.0%
GTAATATGTAAGACGATGAA+AGG + chr7_4:49325783-49325802 None:intergenic 30.0%
! TCTTCTCAGTTTTTGTGTTG+TGG + chr7_4:49323589-49323608 None:intergenic 35.0%
CGTACAAAATAGCGAAAAGA+AGG + chr7_4:49323753-49323772 None:intergenic 35.0%
!! GCTATTTTGTACGTTGAACA+CGG - chr7_4:49323760-49323779 Msa1159670:three_prime_UTR 35.0%
ATAGGGTTTATGCGAAAAGA+GGG + chr7_4:49323919-49323938 None:intergenic 35.0%
AATAGGGTTTATGCGAAAAG+AGG + chr7_4:49323920-49323939 None:intergenic 35.0%
AACACACAGACTCCTTAAAT+TGG + chr7_4:49324113-49324132 None:intergenic 35.0%
TCAAACCTAATAGACCATAG+AGG + chr7_4:49324145-49324164 None:intergenic 35.0%
CTATTCAATTGTCCTTGATC+CGG - chr7_4:49324187-49324206 Msa1159670:intron 35.0%
TGACATCGAATTCAAAAGAC+CGG + chr7_4:49324209-49324228 None:intergenic 35.0%
!! TGTCATAAACAGTTTGGTGT+TGG - chr7_4:49324224-49324243 Msa1159670:intron 35.0%
! TGGTGTTGGAATTATACTCT+TGG - chr7_4:49324238-49324257 Msa1159670:intron 35.0%
! CGATTGTCGTTTAAAGCTTA+AGG + chr7_4:49324408-49324427 None:intergenic 35.0%
!! TTTGGTTTGGGAAAACTAGT+GGG + chr7_4:49324495-49324514 None:intergenic 35.0%
! TACACATACCACTTTGGTTT+GGG + chr7_4:49324507-49324526 None:intergenic 35.0%
ATACACATACCACTTTGGTT+TGG + chr7_4:49324508-49324527 None:intergenic 35.0%
GTGTATATATGTAGGTACAG+GGG - chr7_4:49324522-49324541 Msa1159670:intron 35.0%
GGAAAAGGAATGCTAAAGAA+AGG - chr7_4:49324595-49324614 Msa1159670:intron 35.0%
TTACTGCTCTCAATTTGGTT+TGG + chr7_4:49324636-49324655 None:intergenic 35.0%
TCGTATGATTTCAATGAGAG+TGG - chr7_4:49324676-49324695 Msa1159670:intron 35.0%
TTGAGGTGTATTCAGAAAGA+TGG - chr7_4:49325055-49325074 Msa1159670:intron 35.0%
GAAAGATGGTGACAATTGAA+TGG - chr7_4:49325069-49325088 Msa1159670:intron 35.0%
GTGGGAATAATTTGATCCAT+TGG - chr7_4:49325155-49325174 Msa1159670:intron 35.0%
CAAAGAATTTCATAGTTGCC+GGG - chr7_4:49325318-49325337 Msa1159670:intron 35.0%
AAAGAATTTCATAGTTGCCG+GGG - chr7_4:49325319-49325338 Msa1159670:intron 35.0%
! GGTTTGACAACTTTTGTCTT+TGG - chr7_4:49325341-49325360 Msa1159670:intron 35.0%
! TTTGGTTGTACGCAATGTTT+TGG - chr7_4:49325516-49325535 Msa1159670:intron 35.0%
!!! TTGTACGCAATGTTTTGGTT+TGG - chr7_4:49325521-49325540 Msa1159670:intron 35.0%
!!! TGTACGCAATGTTTTGGTTT+GGG - chr7_4:49325522-49325541 Msa1159670:intron 35.0%
AACTATTTCCATGCTGAAAG+GGG + chr7_4:49325562-49325581 None:intergenic 35.0%
CGACAAATGGGGAAAAATAA+TGG + chr7_4:49325748-49325767 None:intergenic 35.0%
AAAATGAAGTGCGACAAATG+GGG + chr7_4:49325759-49325778 None:intergenic 35.0%
GAAAAATGAAGTGCGACAAA+TGG + chr7_4:49325761-49325780 None:intergenic 35.0%
! TCTTCTCAGTTTTTGTGTTG+TGG + chr7_4:49323589-49323608 None:intergenic 35.0%
CGTACAAAATAGCGAAAAGA+AGG + chr7_4:49323753-49323772 None:intergenic 35.0%
!! GCTATTTTGTACGTTGAACA+CGG - chr7_4:49323760-49323779 Msa1159670:three_prime_UTR 35.0%
ATAGGGTTTATGCGAAAAGA+GGG + chr7_4:49323919-49323938 None:intergenic 35.0%
AATAGGGTTTATGCGAAAAG+AGG + chr7_4:49323920-49323939 None:intergenic 35.0%
AACACACAGACTCCTTAAAT+TGG + chr7_4:49324113-49324132 None:intergenic 35.0%
TCAAACCTAATAGACCATAG+AGG + chr7_4:49324145-49324164 None:intergenic 35.0%
CTATTCAATTGTCCTTGATC+CGG - chr7_4:49324187-49324206 Msa1159670:intron 35.0%
TGACATCGAATTCAAAAGAC+CGG + chr7_4:49324209-49324228 None:intergenic 35.0%
!! TGTCATAAACAGTTTGGTGT+TGG - chr7_4:49324224-49324243 Msa1159670:intron 35.0%
! TGGTGTTGGAATTATACTCT+TGG - chr7_4:49324238-49324257 Msa1159670:intron 35.0%
! CGATTGTCGTTTAAAGCTTA+AGG + chr7_4:49324408-49324427 None:intergenic 35.0%
!! TTTGGTTTGGGAAAACTAGT+GGG + chr7_4:49324495-49324514 None:intergenic 35.0%
! TACACATACCACTTTGGTTT+GGG + chr7_4:49324507-49324526 None:intergenic 35.0%
ATACACATACCACTTTGGTT+TGG + chr7_4:49324508-49324527 None:intergenic 35.0%
GTGTATATATGTAGGTACAG+GGG - chr7_4:49324522-49324541 Msa1159670:intron 35.0%
GGAAAAGGAATGCTAAAGAA+AGG - chr7_4:49324595-49324614 Msa1159670:intron 35.0%
TTACTGCTCTCAATTTGGTT+TGG + chr7_4:49324636-49324655 None:intergenic 35.0%
TCGTATGATTTCAATGAGAG+TGG - chr7_4:49324676-49324695 Msa1159670:intron 35.0%
TTGAGGTGTATTCAGAAAGA+TGG - chr7_4:49325055-49325074 Msa1159670:intron 35.0%
GAAAGATGGTGACAATTGAA+TGG - chr7_4:49325069-49325088 Msa1159670:intron 35.0%
GTGGGAATAATTTGATCCAT+TGG - chr7_4:49325155-49325174 Msa1159670:intron 35.0%
CAAAGAATTTCATAGTTGCC+GGG - chr7_4:49325318-49325337 Msa1159670:intron 35.0%
AAAGAATTTCATAGTTGCCG+GGG - chr7_4:49325319-49325338 Msa1159670:intron 35.0%
! GGTTTGACAACTTTTGTCTT+TGG - chr7_4:49325341-49325360 Msa1159670:intron 35.0%
! TTTGGTTGTACGCAATGTTT+TGG - chr7_4:49325516-49325535 Msa1159670:intron 35.0%
!!! TTGTACGCAATGTTTTGGTT+TGG - chr7_4:49325521-49325540 Msa1159670:intron 35.0%
!!! TGTACGCAATGTTTTGGTTT+GGG - chr7_4:49325522-49325541 Msa1159670:intron 35.0%
AACTATTTCCATGCTGAAAG+GGG + chr7_4:49325562-49325581 None:intergenic 35.0%
CGACAAATGGGGAAAAATAA+TGG + chr7_4:49325748-49325767 None:intergenic 35.0%
AAAATGAAGTGCGACAAATG+GGG + chr7_4:49325759-49325778 None:intergenic 35.0%
GAAAAATGAAGTGCGACAAA+TGG + chr7_4:49325761-49325780 None:intergenic 35.0%
!! GGTGCTGTTTACAAGAGAAA+GGG + chr7_4:49323565-49323584 None:intergenic 40.0%
! TCTCAGTTTTTGTGTTGTGG+CGG + chr7_4:49323586-49323605 None:intergenic 40.0%
AGAAGAGAAAGAGAAAGCGA+CGG + chr7_4:49323711-49323730 None:intergenic 40.0%
TAGGGTTTATGCGAAAAGAG+GGG + chr7_4:49323918-49323937 None:intergenic 40.0%
TCAACCACAGACAACAAACT+AGG + chr7_4:49324064-49324083 None:intergenic 40.0%
GTGTTAGAATCAGACCTCTA+TGG - chr7_4:49324128-49324147 Msa1159670:exon 40.0%
TCAGACCTCTATGGTCTATT+AGG - chr7_4:49324137-49324156 Msa1159670:intron 40.0%
CGGATCAAGGACAATTGAAT+AGG + chr7_4:49324189-49324208 None:intergenic 40.0%
GAATTCAAAAGACCGGATCA+AGG + chr7_4:49324202-49324221 None:intergenic 40.0%
!! TTGGTTTGGGAAAACTAGTG+GGG + chr7_4:49324494-49324513 None:intergenic 40.0%
!! CTTTGGTTTGGGAAAACTAG+TGG + chr7_4:49324496-49324515 None:intergenic 40.0%
!! CTAGTTTTCCCAAACCAAAG+TGG - chr7_4:49324496-49324515 Msa1159670:intron 40.0%
AAACAAGAGGCTAAGATGTG+AGG - chr7_4:49324574-49324593 Msa1159670:intron 40.0%
AGATTAGGAAAGCAACTGTG+AGG - chr7_4:49324832-49324851 Msa1159670:intron 40.0%
! ACCAAAATGGCTGGAGTATT+AGG - chr7_4:49325272-49325291 Msa1159670:intron 40.0%
! TCCTAATACTCCAGCCATTT+TGG + chr7_4:49325276-49325295 None:intergenic 40.0%
! GCTGGAGTATTAGGATATAG+TGG - chr7_4:49325281-49325300 Msa1159670:intron 40.0%
AGTATTAGGATATAGTGGCC+TGG - chr7_4:49325286-49325305 Msa1159670:intron 40.0%
AAGAATTTCATAGTTGCCGG+GGG - chr7_4:49325320-49325339 Msa1159670:intron 40.0%
! AATGCTTGATTCGCCTTCTT+TGG + chr7_4:49325450-49325469 None:intergenic 40.0%
CGAATCAAGCATTCCATCAA+AGG - chr7_4:49325457-49325476 Msa1159670:intron 40.0%
ACTGTAAGCAACTAAGCTCT+AGG + chr7_4:49325483-49325502 None:intergenic 40.0%
ACGTGAGACTCAATATCTCT+TGG - chr7_4:49325695-49325714 Msa1159670:five_prime_UTR 40.0%
!! GGTGCTGTTTACAAGAGAAA+GGG + chr7_4:49323565-49323584 None:intergenic 40.0%
! TCTCAGTTTTTGTGTTGTGG+CGG + chr7_4:49323586-49323605 None:intergenic 40.0%
AGAAGAGAAAGAGAAAGCGA+CGG + chr7_4:49323711-49323730 None:intergenic 40.0%
TAGGGTTTATGCGAAAAGAG+GGG + chr7_4:49323918-49323937 None:intergenic 40.0%
TCAACCACAGACAACAAACT+AGG + chr7_4:49324064-49324083 None:intergenic 40.0%
GTGTTAGAATCAGACCTCTA+TGG - chr7_4:49324128-49324147 Msa1159670:exon 40.0%
TCAGACCTCTATGGTCTATT+AGG - chr7_4:49324137-49324156 Msa1159670:intron 40.0%
CGGATCAAGGACAATTGAAT+AGG + chr7_4:49324189-49324208 None:intergenic 40.0%
GAATTCAAAAGACCGGATCA+AGG + chr7_4:49324202-49324221 None:intergenic 40.0%
!! TTGGTTTGGGAAAACTAGTG+GGG + chr7_4:49324494-49324513 None:intergenic 40.0%
!! CTTTGGTTTGGGAAAACTAG+TGG + chr7_4:49324496-49324515 None:intergenic 40.0%
!! CTAGTTTTCCCAAACCAAAG+TGG - chr7_4:49324496-49324515 Msa1159670:intron 40.0%
AAACAAGAGGCTAAGATGTG+AGG - chr7_4:49324574-49324593 Msa1159670:intron 40.0%
AGATTAGGAAAGCAACTGTG+AGG - chr7_4:49324832-49324851 Msa1159670:intron 40.0%
! ACCAAAATGGCTGGAGTATT+AGG - chr7_4:49325272-49325291 Msa1159670:intron 40.0%
! TCCTAATACTCCAGCCATTT+TGG + chr7_4:49325276-49325295 None:intergenic 40.0%
! GCTGGAGTATTAGGATATAG+TGG - chr7_4:49325281-49325300 Msa1159670:intron 40.0%
AGTATTAGGATATAGTGGCC+TGG - chr7_4:49325286-49325305 Msa1159670:intron 40.0%
AAGAATTTCATAGTTGCCGG+GGG - chr7_4:49325320-49325339 Msa1159670:intron 40.0%
! AATGCTTGATTCGCCTTCTT+TGG + chr7_4:49325450-49325469 None:intergenic 40.0%
CGAATCAAGCATTCCATCAA+AGG - chr7_4:49325457-49325476 Msa1159670:intron 40.0%
ACTGTAAGCAACTAAGCTCT+AGG + chr7_4:49325483-49325502 None:intergenic 40.0%
ACGTGAGACTCAATATCTCT+TGG - chr7_4:49325695-49325714 Msa1159670:five_prime_UTR 40.0%
!! CGGTGCTGTTTACAAGAGAA+AGG + chr7_4:49323566-49323585 None:intergenic 45.0%
TTGTGAAATGAGTGGCAGCT+TGG + chr7_4:49323635-49323654 None:intergenic 45.0%
! GTCGCTTTCTCTTTCTCTTC+TGG - chr7_4:49323710-49323729 Msa1159670:three_prime_UTR 45.0%
!! TGCTCCTAGTTTGTTGTCTG+TGG - chr7_4:49324057-49324076 Msa1159670:CDS 45.0%
GAGGCTAAGATGTGAGGAAA+AGG - chr7_4:49324580-49324599 Msa1159670:intron 45.0%
AATTCTTTGTCTTAGGCGCC+AGG + chr7_4:49325307-49325326 None:intergenic 45.0%
GACAAAAGTTGTCAAACCCC+CGG + chr7_4:49325339-49325358 None:intergenic 45.0%
TTCTACACCATGAGAGCAGT+TGG - chr7_4:49325371-49325390 Msa1159670:intron 45.0%
!! TGGAGGTACTGATGAACTAC+AGG - chr7_4:49325391-49325410 Msa1159670:intron 45.0%
!! ACTAAGCTCTAGGCCTTTGA+TGG + chr7_4:49325473-49325492 None:intergenic 45.0%
! GCTTTTAACCCCTTTCAGCA+TGG - chr7_4:49325551-49325570 Msa1159670:intron 45.0%
!! CGGTGCTGTTTACAAGAGAA+AGG + chr7_4:49323566-49323585 None:intergenic 45.0%
TTGTGAAATGAGTGGCAGCT+TGG + chr7_4:49323635-49323654 None:intergenic 45.0%
! GTCGCTTTCTCTTTCTCTTC+TGG - chr7_4:49323710-49323729 Msa1159670:three_prime_UTR 45.0%
!! TGCTCCTAGTTTGTTGTCTG+TGG - chr7_4:49324057-49324076 Msa1159670:CDS 45.0%
GAGGCTAAGATGTGAGGAAA+AGG - chr7_4:49324580-49324599 Msa1159670:intron 45.0%
AATTCTTTGTCTTAGGCGCC+AGG + chr7_4:49325307-49325326 None:intergenic 45.0%
GACAAAAGTTGTCAAACCCC+CGG + chr7_4:49325339-49325358 None:intergenic 45.0%
TTCTACACCATGAGAGCAGT+TGG - chr7_4:49325371-49325390 Msa1159670:intron 45.0%
!! TGGAGGTACTGATGAACTAC+AGG - chr7_4:49325391-49325410 Msa1159670:intron 45.0%
!! ACTAAGCTCTAGGCCTTTGA+TGG + chr7_4:49325473-49325492 None:intergenic 45.0%
! GCTTTTAACCCCTTTCAGCA+TGG - chr7_4:49325551-49325570 Msa1159670:intron 45.0%
TTACTAAAACGCCGCCGTAG+AGG - chr7_4:49323660-49323679 Msa1159670:three_prime_UTR 50.0%
GGTGTGTACTGAGTGAGCAA+AGG - chr7_4:49324368-49324387 Msa1159670:intron 50.0%
GTAGGTACAGGGGTAGTTCA+AGG - chr7_4:49324532-49324551 Msa1159670:intron 50.0%
TACACCATGAGAGCAGTTGG+AGG - chr7_4:49325374-49325393 Msa1159670:intron 50.0%
AGTACCTCCAACTGCTCTCA+TGG + chr7_4:49325381-49325400 None:intergenic 50.0%
!! GCCTTCTTTGGTGCTCTTGT+CGG + chr7_4:49325438-49325457 None:intergenic 50.0%
TTACTAAAACGCCGCCGTAG+AGG - chr7_4:49323660-49323679 Msa1159670:three_prime_UTR 50.0%
GGTGTGTACTGAGTGAGCAA+AGG - chr7_4:49324368-49324387 Msa1159670:intron 50.0%
GTAGGTACAGGGGTAGTTCA+AGG - chr7_4:49324532-49324551 Msa1159670:intron 50.0%
TACACCATGAGAGCAGTTGG+AGG - chr7_4:49325374-49325393 Msa1159670:intron 50.0%
AGTACCTCCAACTGCTCTCA+TGG + chr7_4:49325381-49325400 None:intergenic 50.0%
!! GCCTTCTTTGGTGCTCTTGT+CGG + chr7_4:49325438-49325457 None:intergenic 50.0%
AGAAAGAGAAAGCGACGGCG+AGG + chr7_4:49323706-49323725 None:intergenic 55.0%
!! GCCGACAAGAGCACCAAAGA+AGG - chr7_4:49325434-49325453 Msa1159670:intron 55.0%
AGAAAGAGAAAGCGACGGCG+AGG + chr7_4:49323706-49323725 None:intergenic 55.0%
!! GCCGACAAGAGCACCAAAGA+AGG - chr7_4:49325434-49325453 Msa1159670:intron 55.0%
AAGAGAAAGCGACGGCGAGG+TGG + chr7_4:49323703-49323722 None:intergenic 60.0%
AAGAGAAAGCGACGGCGAGG+TGG + chr7_4:49323703-49323722 None:intergenic 60.0%
CGGTCTTGGGTCCTCTACGG+CGG + chr7_4:49323674-49323693 None:intergenic 65.0%
CGGCGGTCTTGGGTCCTCTA+CGG + chr7_4:49323677-49323696 None:intergenic 65.0%
CGGTCTTGGGTCCTCTACGG+CGG + chr7_4:49323674-49323693 None:intergenic 65.0%
CGGCGGTCTTGGGTCCTCTA+CGG + chr7_4:49323677-49323696 None:intergenic 65.0%
GAGGTGGACGCGGCGGTCTT+GGG + chr7_4:49323687-49323706 None:intergenic 70.0%
AAGCGACGGCGAGGTGGACG+CGG + chr7_4:49323697-49323716 None:intergenic 70.0%
GAGGTGGACGCGGCGGTCTT+GGG + chr7_4:49323687-49323706 None:intergenic 70.0%
AAGCGACGGCGAGGTGGACG+CGG + chr7_4:49323697-49323716 None:intergenic 70.0%
CGAGGTGGACGCGGCGGTCT+TGG + chr7_4:49323688-49323707 None:intergenic 75.0%
CGAGGTGGACGCGGCGGTCT+TGG + chr7_4:49323688-49323707 None:intergenic 75.0%
! CGACGGCGAGGTGGACGCGG+CGG + chr7_4:49323694-49323713 None:intergenic 80.0%
! CGACGGCGAGGTGGACGCGG+CGG + chr7_4:49323694-49323713 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 49323538 49325883 49323538 ID=Msa1159670;Name=Msa1159670
chr7_4 mRNA 49323538 49325883 49323538 ID=Msa1159670-mRNA-1;Parent=Msa1159670;Name=Msa1159670-mRNA-1;_AED=0.28;_eAED=0.28;_QI=203|1|1|1|0|0.5|2|399|68
chr7_4 exon 49323538 49324152 49323538 ID=Msa1159670-mRNA-1:exon:13582;Parent=Msa1159670-mRNA-1
chr7_4 exon 49325690 49325883 49325690 ID=Msa1159670-mRNA-1:exon:13581;Parent=Msa1159670-mRNA-1
chr7_4 five_prime_UTR 49325690 49325883 49325690 ID=Msa1159670-mRNA-1:five_prime_utr;Parent=Msa1159670-mRNA-1
chr7_4 five_prime_UTR 49324144 49324152 49324144 ID=Msa1159670-mRNA-1:five_prime_utr;Parent=Msa1159670-mRNA-1
chr7_4 CDS 49323937 49324143 49323937 ID=Msa1159670-mRNA-1:cds;Parent=Msa1159670-mRNA-1
chr7_4 three_prime_UTR 49323538 49323936 49323538 ID=Msa1159670-mRNA-1:three_prime_utr;Parent=Msa1159670-mRNA-1
Gene Sequence

>Msa1159670

ATGGCTGGAGTATTAGGATATAGTGGCCTGGCGCCTAAGACAAAGAATTTCATAGTTGCCGGGGGTTTGACAACTTTTGTCTTTGGGGCATACTTCTACACCATGAGAGCAGTTGGAGGTACTGATGAACTACAGGTAGCAATAGATAAGTTTGAAGCCGACAAGAGCACCAAAGAAGGCGAATCAAGCATTCCATCAAAGGCCTAG
Protein sequence

>Msa1159670

MAGVLGYSGLAPKTKNFIVAGGLTTFVFGAYFYTMRAVGGTDELQVAIDKFEADKSTKEGESSIPSKA*