AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1081750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1081750 MtrunA17_Chr7g0268961 98.475 459 6 1 45 502 32 490 0.0 947
Msa1081750 MtrunA17_Chr1g0191991 56.154 390 113 9 1 350 1 372 4.21e-125 375
Msa1081750 MtrunA17_Chr2g0330171 56.000 300 82 5 45 329 56 320 2.33e-107 328
Msa1081750 MtrunA17_Chr8g0342421 50.263 380 149 9 1 357 18 380 1.44e-105 325
Msa1081750 MtrunA17_Chr7g0263941 48.872 399 155 10 3 387 2 365 4.09e-104 317
Msa1081750 MtrunA17_Chr4g0040571 50.278 360 107 8 45 333 49 407 9.23e-104 319
Msa1081750 MtrunA17_Chr8g0373541 71.859 199 44 2 1 197 7 195 5.39e-103 318
Msa1081750 MtrunA17_Chr1g0181381 69.524 210 60 2 1 206 1 210 1.87e-101 313
Msa1081750 MtrunA17_Chr1g0185121 55.184 299 118 4 45 338 50 337 9.22e-101 310
Msa1081750 MtrunA17_Chr1g0210821 83.007 153 26 0 45 197 54 206 4.52e-99 310
Msa1081750 MtrunA17_Chr1g0163001 83.553 152 25 0 46 197 54 205 2.48e-97 303
Msa1081750 MtrunA17_Chr1g0199101 82.667 150 26 0 45 194 57 206 2.63e-97 304
Msa1081750 MtrunA17_Chr4g0029521 81.290 155 28 1 45 198 48 202 5.94e-96 299
Msa1081750 MtrunA17_Chr1g0162971 79.739 153 31 0 45 197 51 203 7.49e-96 297
Msa1081750 MtrunA17_Chr1g0151351 50.321 312 105 6 45 350 51 318 1.89e-95 296
Msa1081750 MtrunA17_Chr2g0326381 78.065 155 33 1 45 198 42 196 8.61e-93 288
Msa1081750 MtrunA17_Chr3g0141431 49.363 314 110 7 45 333 32 321 4.28e-90 281
Msa1081750 MtrunA17_Chr2g0324991 85.496 131 19 0 46 176 3 133 4.71e-83 254
Msa1081750 MtrunA17_Chr8g0375521 63.125 160 54 3 31 188 26 182 7.49e-69 226
Msa1081750 MtrunA17_Chr6g0479921 61.250 160 58 2 31 189 28 184 7.52e-69 228
Msa1081750 MtrunA17_Chr1g0200421 33.898 177 96 6 49 209 236 407 1.34e-24 107
Msa1081750 MtrunA17_Chr2g0320341 34.000 150 85 3 49 184 229 378 5.63e-23 100
Msa1081750 MtrunA17_Chr3g0123941 35.762 151 89 3 45 187 118 268 7.49e-23 99.4
Msa1081750 MtrunA17_Chr8g0352011 33.333 216 111 11 2 195 122 326 1.20e-21 96.3
Msa1081750 MtrunA17_Chr7g0258011 34.132 167 86 6 46 195 177 336 3.28e-21 95.1
Msa1081750 MtrunA17_Chr8g0382121 35.811 148 84 5 48 184 109 256 8.00e-21 93.2
Msa1081750 MtrunA17_Chr5g0422571 36.552 145 79 6 57 190 107 249 1.22e-20 92.4
Msa1081750 MtrunA17_Chr1g0173841 35.099 151 82 5 46 187 216 359 6.25e-20 91.7
Msa1081750 MtrunA17_Chr2g0328921 35.526 152 80 7 46 187 139 282 9.61e-20 90.1
Msa1081750 MtrunA17_Chr1g0198651 33.537 164 86 7 58 200 109 270 1.27e-19 89.4
Msa1081750 MtrunA17_Chr4g0026521 36.170 141 78 6 57 187 124 262 3.54e-19 88.2
Msa1081750 MtrunA17_Chr2g0321291 30.198 202 112 8 1 187 90 277 7.99e-19 87.4
Msa1081750 MtrunA17_Chr4g0053341 33.553 152 83 6 46 187 75 218 2.51e-18 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1081750 AT3G50700.1 54.489 323 117 6 45 346 44 357 9.13e-107 326
Msa1081750 AT1G03840.1 86.452 155 21 0 45 199 51 205 7.39e-101 312
Msa1081750 AT1G03840.2 85.806 155 22 0 45 199 49 203 4.06e-100 310
Msa1081750 AT5G66730.1 70.918 196 47 1 2 197 9 194 1.79e-99 309
Msa1081750 AT5G44160.2 73.545 189 42 2 45 225 8 196 5.97e-99 305
Msa1081750 AT5G44160.1 73.545 189 42 2 45 225 47 235 1.21e-98 305
Msa1081750 AT1G55110.3 69.608 204 56 3 1 198 24 227 1.40e-98 305
Msa1081750 AT1G55110.1 69.608 204 56 3 1 198 24 227 1.40e-98 305
Msa1081750 AT1G55110.2 69.608 204 56 3 1 198 24 227 1.40e-98 305
Msa1081750 AT1G14580.1 82.353 153 27 0 45 197 63 215 7.58e-98 303
Msa1081750 AT1G14580.2 82.353 153 27 0 45 197 63 215 7.58e-98 303
Msa1081750 AT1G14580.3 82.353 153 27 0 45 197 77 229 1.06e-97 303
Msa1081750 AT2G02070.2 82.166 157 28 0 48 204 65 221 1.23e-97 307
Msa1081750 AT2G02070.1 82.166 157 28 0 48 204 65 221 1.23e-97 307
Msa1081750 AT2G02080.1 81.699 153 28 0 45 197 64 216 2.04e-97 304
Msa1081750 AT2G02080.4 81.699 153 28 0 45 197 64 216 2.04e-97 304
Msa1081750 AT2G02080.5 81.699 153 28 0 45 197 64 216 2.04e-97 304
Msa1081750 AT2G02080.3 81.699 153 28 0 45 197 64 216 2.04e-97 304
Msa1081750 AT5G03150.1 84.516 155 23 1 45 198 63 217 4.69e-97 303
Msa1081750 AT3G45260.2 82.468 154 26 1 45 197 49 202 9.07e-96 297
Msa1081750 AT3G45260.1 82.468 154 26 1 45 197 49 202 9.07e-96 297
Msa1081750 AT3G13810.6 65.138 218 48 3 1 197 4 214 3.85e-95 297
Msa1081750 AT3G13810.5 65.138 218 48 3 1 197 18 228 4.92e-95 297
Msa1081750 AT3G13810.4 65.138 218 48 3 1 197 23 233 7.39e-95 297
Msa1081750 AT3G13810.1 65.138 218 48 3 1 197 23 233 7.39e-95 297
Msa1081750 AT3G13810.3 84.314 153 23 1 46 197 68 220 4.71e-94 295
Msa1081750 AT4G02670.1 65.297 219 59 4 8 211 17 233 4.73e-94 291
Msa1081750 AT4G02670.2 65.297 219 59 4 8 211 19 235 5.31e-94 291
Msa1081750 AT3G13810.2 84.314 153 23 1 46 197 82 234 6.97e-94 295
Msa1081750 AT5G60470.2 78.065 155 33 1 45 198 54 208 8.80e-92 287
Msa1081750 AT5G60470.3 78.065 155 33 1 45 198 50 204 8.82e-92 287
Msa1081750 AT5G60470.1 78.431 153 32 1 46 197 46 198 2.35e-91 286
Msa1081750 AT2G02080.6 82.014 139 25 0 59 197 1 139 1.21e-89 281
Msa1081750 AT2G02080.2 82.014 139 25 0 59 197 1 139 1.21e-89 281
Msa1081750 AT1G68130.1 67.808 146 45 2 45 188 51 196 1.34e-69 229
Msa1081750 AT2G01940.1 66.892 148 47 2 45 190 54 201 3.31e-69 228
Msa1081750 AT1G25250.1 64.865 148 49 2 44 188 19 166 3.31e-68 223
Msa1081750 AT1G25250.3 63.226 155 51 3 37 188 38 189 3.81e-68 224
Msa1081750 AT2G01940.3 65.772 149 48 3 45 190 54 202 2.67e-65 218
Msa1081750 AT1G68130.2 65.455 110 36 2 81 188 1 110 9.64e-47 166
Msa1081750 AT2G01940.2 65.179 112 37 2 81 190 1 112 1.26e-46 166
Msa1081750 AT1G25250.2 62.162 111 39 2 81 188 1 111 4.39e-45 160
Msa1081750 AT1G34370.3 33.333 153 88 3 49 187 19 171 2.97e-23 100
Msa1081750 AT5G22890.1 34.000 150 85 3 49 184 202 351 3.08e-23 101
Msa1081750 AT1G34370.2 33.333 153 88 3 49 187 229 381 6.89e-23 102
Msa1081750 AT1G34370.1 33.333 153 88 3 49 187 229 381 6.89e-23 102
Msa1081750 AT3G20880.1 37.143 140 76 6 58 187 250 387 5.38e-20 92.4
Msa1081750 AT1G51220.1 33.775 151 84 5 46 187 166 309 1.04e-19 90.9
Msa1081750 AT3G57670.1 34.437 151 83 5 46 187 205 348 3.78e-19 89.7
Msa1081750 AT1G13290.1 35.862 145 79 7 58 190 95 237 4.88e-19 88.2
Msa1081750 AT1G08290.1 35.294 136 75 5 63 187 179 312 1.61e-17 84.3
Msa1081750 AT1G34790.1 33.333 150 88 6 48 187 129 276 1.10e-16 81.3

Find 104 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGTCCTAATCATGTTTC+TGG 0.126378 7_2:-81645385 Msa1081750:CDS
ATTATCTCCAACAACATTAA+TGG 0.225501 7_2:-81646639 Msa1081750:CDS
ATTGAAATGTCAAATATTTC+AGG 0.250060 7_2:-81647653 None:intergenic
TGCATTTGGCTTTGCAAGTT+TGG 0.264117 7_2:+81645805 None:intergenic
CAGCAATGGAGAAATCAATT+TGG 0.264385 7_2:-81644949 Msa1081750:CDS
ATTGCTGTACCTAGAAAATA+TGG 0.277676 7_2:+81646277 None:intergenic
GTCCATGATTTCTTGGGAAT+TGG 0.293303 7_2:-81645082 Msa1081750:CDS
GGCAACAGTCCATGATTTCT+TGG 0.325473 7_2:-81645089 Msa1081750:CDS
TTGGAGCATGTTCTTGAGTT+GGG 0.332417 7_2:+81645692 None:intergenic
GTAATTGAAGCTGTTGGAAT+TGG 0.337918 7_2:+81645555 None:intergenic
TCTACAATGAATAATGATAT+TGG 0.357969 7_2:-81645190 Msa1081750:CDS
TTTAGAGGCATGGTAGAGAT+AGG 0.380908 7_2:+81645778 None:intergenic
TTTGTTGCCATTAATGTTGT+TGG 0.382808 7_2:+81646632 None:intergenic
TCTGAGGAAGTGTTGGAAAC+AGG 0.383083 7_2:+81645754 None:intergenic
AGCAATGGAGAAATCAATTT+GGG 0.385884 7_2:-81644948 Msa1081750:CDS
GTTCATAAGAGGGTTGGTTT+TGG 0.388862 7_2:+81645341 None:intergenic
GCAAACATGGTGAGAAGAAA+TGG 0.396336 7_2:-81646397 Msa1081750:CDS
CGTGCATTAGGTGACCTTAC+TGG 0.399967 7_2:-81646440 Msa1081750:CDS
TTCGATGTAACTGAAGATTC+TGG 0.401666 7_2:+81646567 None:intergenic
AGTGGAACCATTGCTATTGC+AGG 0.405406 7_2:+81647529 None:intergenic
CACAATCCAGCTCGTGCATT+AGG 0.407149 7_2:-81646452 Msa1081750:CDS
AATCTCAATGACTCAAGTTC+AGG 0.409366 7_2:-81647563 Msa1081750:CDS
GCATGCCACTGGTTAATCCT+TGG 0.413966 7_2:+81645830 None:intergenic
ATTTCTTCTCACCATGTTTG+CGG 0.414358 7_2:+81646399 None:intergenic
TAGAGATAGGATCATGCATT+TGG 0.417757 7_2:+81645791 None:intergenic
TGGATTAAAGTGTTCATAAG+AGG 0.422907 7_2:+81645330 None:intergenic
TGAACCACAGCAACAAAGAT+TGG 0.429733 7_2:-81645038 Msa1081750:CDS
GATATGCTGTTCAATCTGAT+TGG 0.431168 7_2:-81646352 Msa1081750:CDS
ACCAGAAAACATGTGGTACT+AGG 0.442303 7_2:-81646322 Msa1081750:CDS
GACTCAAGTTCAGGTCCATC+TGG 0.442408 7_2:-81647554 Msa1081750:CDS
CATGATTTCTTGGGAATTGG+AGG 0.445123 7_2:-81645079 Msa1081750:CDS
GCAACAGTCCATGATTTCTT+GGG 0.459975 7_2:-81645088 Msa1081750:CDS
CATGCCACCACATGCATTGT+TGG 0.462483 7_2:+81645654 None:intergenic
CTCCAATTCCCAAGAAATCA+TGG 0.464360 7_2:+81645080 None:intergenic
AGTTGAAGGGTTGAAGAAGA+TGG 0.465582 7_2:+81645583 None:intergenic
TCAAATATTTCAGGTGATGA+AGG 0.466760 7_2:-81647644 Msa1081750:CDS
ATAGAACCAGAAACATGATT+AGG 0.468762 7_2:+81645379 None:intergenic
GGTGCTACTGCAAGCAATAG+TGG 0.468938 7_2:-81645448 Msa1081750:CDS
TCTAACATGGCTGGTGTTAG+TGG 0.470215 7_2:-81645292 Msa1081750:CDS
TGGAATTGGAACTAAGTTGA+AGG 0.481061 7_2:+81645569 None:intergenic
GTTGGAGCATGTTCTTGAGT+TGG 0.484302 7_2:+81645691 None:intergenic
GATATTGGAATGTTTAGTCA+TGG 0.486932 7_2:-81645175 Msa1081750:CDS
GCTTCCAATCTTTGTTGCTG+TGG 0.487682 7_2:+81645034 None:intergenic
AATTGTTCAAATTTGATACA+TGG 0.491018 7_2:-81645121 Msa1081750:CDS
AGCATTAAGTCAACAGAGGT+TGG 0.491230 7_2:-81645014 Msa1081750:CDS
GTTTAATTCTGAGGAAGTGT+TGG 0.503695 7_2:+81645747 None:intergenic
CAAGTTTGGTGGCATGCCAC+TGG 0.504812 7_2:+81645819 None:intergenic
ACAATCTTCCATGGAAACTT+AGG 0.513491 7_2:-81646538 Msa1081750:CDS
TAAAGTGTTCATAAGAGGGT+TGG 0.514305 7_2:+81645335 None:intergenic
GATGAAGAATGTTGCACAAG+AGG 0.514880 7_2:-81645149 Msa1081750:CDS
TTGGTTGATCTGAATGATGT+TGG 0.518626 7_2:+81645673 None:intergenic
GTTCTTTGCCTAAGTTTCCA+TGG 0.520812 7_2:+81646530 None:intergenic
ATTGAAGTAATTGAAGCTGT+TGG 0.522656 7_2:+81645549 None:intergenic
GAAAAGCTTTGTTAGTAGCA+TGG 0.526473 7_2:-81645407 Msa1081750:CDS
TGGAGGACTAATTGCTGGCA+TGG 0.527954 7_2:-81645515 Msa1081750:CDS
CCAGAAAACATGTGGTACTA+GGG 0.530598 7_2:-81646321 Msa1081750:CDS
ATTTGGCTTTGCAAGTTTGG+TGG 0.531585 7_2:+81645808 None:intergenic
AGCTTCTCCTCAGGAAACAA+TGG 0.532782 7_2:-81647620 Msa1081750:CDS
TGGTGAACACATGATGGCTC+AGG 0.534078 7_2:+81646473 None:intergenic
TGCTGTTCAATCTGATTGGA+AGG 0.534743 7_2:-81646348 Msa1081750:CDS
TTCTTCTCCATTGTTTCCTG+AGG 0.534803 7_2:+81647613 None:intergenic
ATAGTCATTGTTTAATTCTG+AGG 0.543313 7_2:+81645738 None:intergenic
GATGAAGGAAGCTTCTCCTC+AGG 0.547390 7_2:-81647629 Msa1081750:CDS
GGTTGAAGAAGATGGAGGCA+AGG 0.548129 7_2:+81645591 None:intergenic
ATGGCTGGTGTTAGTGGTGG+AGG 0.549455 7_2:-81645286 Msa1081750:CDS
TGCTGGCATGGCTCAAATGT+CGG 0.549729 7_2:-81645503 Msa1081750:CDS
AGGTCACCTAATGCACGAGC+TGG 0.556111 7_2:+81646446 None:intergenic
GGCATGTTCTCAACTAGTGC+AGG 0.558296 7_2:-81645637 Msa1081750:CDS
TCATCATCATCTTCCACATG+AGG 0.560330 7_2:-81644975 Msa1081750:CDS
TGGAGCATGTTCTTGAGTTG+GGG 0.560364 7_2:+81645693 None:intergenic
CATGAATTATCTAACATGGC+TGG 0.563778 7_2:-81645301 Msa1081750:CDS
TTGCTATTGCAGGCACCAGA+TGG 0.567581 7_2:+81647539 None:intergenic
GAAGAGGTCACAATCTTCCA+TGG 0.567686 7_2:-81646547 Msa1081750:CDS
GGATTAAAGTGTTCATAAGA+GGG 0.569779 7_2:+81645331 None:intergenic
TCCATTGCTGAATCCTCATG+TGG 0.577321 7_2:+81644962 None:intergenic
TGCATCACAGAATGCTCTGT+GGG 0.579430 7_2:+81645879 None:intergenic
AACAGATCCTACTGCTGAAG+TGG 0.587709 7_2:-81646666 Msa1081750:intron
AGGGAATACAAATGTGATTG+TGG 0.590905 7_2:-81646302 Msa1081750:CDS
GTGCTACTGCAAGCAATAGT+GGG 0.592488 7_2:-81645447 Msa1081750:CDS
TTAGATAATTCATGCTGCAG+TGG 0.592945 7_2:+81645310 None:intergenic
GGAATTGGAACTAAGTTGAA+GGG 0.596686 7_2:+81645570 None:intergenic
AATGCAACCACTTCAGCAGT+AGG 0.598028 7_2:+81646659 None:intergenic
TGAAGGGTTGAAGAAGATGG+AGG 0.598478 7_2:+81645586 None:intergenic
AGAAGTTAGTAAGCAGCAAG+AGG 0.608407 7_2:-81647594 Msa1081750:CDS
TCCACATGAGGATTCAGCAA+TGG 0.616123 7_2:-81644963 Msa1081750:CDS
TATGCAACAAAGGATTTCAG+AGG 0.622063 7_2:-81646592 Msa1081750:CDS
CTAATGCACGAGCTGGATTG+TGG 0.622870 7_2:+81646453 None:intergenic
TCTGGTGCCTGCAATAGCAA+TGG 0.623066 7_2:-81647536 Msa1081750:intron
AATCTTCAGTTACATCGAAG+AGG 0.634133 7_2:-81646563 Msa1081750:CDS
AACATGGCTGGTGTTAGTGG+TGG 0.642397 7_2:-81645289 Msa1081750:CDS
TCAACCAACAATGCATGTGG+TGG 0.656711 7_2:-81645658 Msa1081750:CDS
GGATTGTGGTGAACACATGA+TGG 0.659836 7_2:+81646467 None:intergenic
ATGCAACAAAGGATTTCAGA+GGG 0.660958 7_2:-81646591 Msa1081750:CDS
AGATCAACCAACAATGCATG+TGG 0.667821 7_2:-81645661 Msa1081750:CDS
GCAGCATGAATTATCTAACA+TGG 0.668171 7_2:-81645305 Msa1081750:CDS
TGGAAGCATTAAGTCAACAG+AGG 0.675968 7_2:-81645018 Msa1081750:CDS
GTGTGTGAGATATGCAACAA+AGG 0.682268 7_2:-81646602 Msa1081750:CDS
TTTGATACATGGAAGTGATG+TGG 0.683521 7_2:-81645110 Msa1081750:CDS
ATGCATCACAGAATGCTCTG+TGG 0.684328 7_2:+81645878 None:intergenic
TAGGCAAAGAACAAGTGCAG+AGG 0.701077 7_2:-81646519 Msa1081750:CDS
GAAAACAACAGAGTAAACCA+AGG 0.707069 7_2:-81645847 Msa1081750:CDS
AAGGCTCACCAGAAAACATG+TGG 0.716558 7_2:-81646329 Msa1081750:CDS
GTAAACCAAGGATTAACCAG+TGG 0.716826 7_2:-81645835 Msa1081750:CDS
GGTGAACACATGATGGCTCA+GGG 0.717782 7_2:+81646474 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTAATAATTTGCATAAT+TGG - chr7_2:81646501-81646520 Msa1081750:CDS 10.0%
!!! ATTTTATATAGTTTCTCTAA+TGG - chr7_2:81645362-81645381 Msa1081750:CDS 15.0%
!! AAAAAAACTTATAGCTTATA+TGG + chr7_2:81645500-81645519 None:intergenic 15.0%
!!! AAAATATGTTTTCCATTTTT+AGG + chr7_2:81646448-81646467 None:intergenic 15.0%
!! TTCAAATAAGTCAATTTAAG+CGG - chr7_2:81645638-81645657 Msa1081750:CDS 20.0%
!! TTATTACTACTTAAAGATGA+AGG + chr7_2:81645675-81645694 None:intergenic 20.0%
!!! TTTCTTTTTAATTACCTCAT+TGG + chr7_2:81645730-81645749 None:intergenic 20.0%
!! GCTAGTAACTTATAAAATAA+AGG + chr7_2:81645844-81645863 None:intergenic 20.0%
!! AAAATACATCATCCATTAAT+CGG + chr7_2:81645897-81645916 None:intergenic 20.0%
!! TGAAACAAAGTTAATACAAT+AGG - chr7_2:81646422-81646441 Msa1081750:CDS 20.0%
!!! TAATATTTAGAATGCATGTT+TGG + chr7_2:81646623-81646642 None:intergenic 20.0%
!! TACTTCAATGACAATAAAAA+TGG - chr7_2:81647052-81647071 Msa1081750:intron 20.0%
!! TCTACAATGAATAATGATAT+TGG - chr7_2:81647397-81647416 Msa1081750:intron 20.0%
!! AATTGTTCAAATTTGATACA+TGG - chr7_2:81647466-81647485 Msa1081750:intron 20.0%
! CTGCAATAAAAATTGAAGTT+AGG + chr7_2:81645149-81645168 None:intergenic 25.0%
! TCTAGGCAAATAAAAATCTT+TGG - chr7_2:81645209-81645228 Msa1081750:CDS 25.0%
! AATAAGCTGAAAACATCTTA+TGG - chr7_2:81645548-81645567 Msa1081750:CDS 25.0%
! TACATAGCACTTCTTAAAAA+AGG + chr7_2:81645817-81645836 None:intergenic 25.0%
! GCATATTATTGTTATGCATA+TGG - chr7_2:81645862-81645881 Msa1081750:CDS 25.0%
! ATTATCTCCAACAACATTAA+TGG - chr7_2:81645948-81645967 Msa1081750:intron 25.0%
!!! TGTTACAAATATTTTTGTGC+AGG - chr7_2:81646372-81646391 Msa1081750:CDS 25.0%
! TAATACAATAGGCCTAAAAA+TGG - chr7_2:81646433-81646452 Msa1081750:CDS 25.0%
! AATTGGATTGAAATCGAATT+TGG - chr7_2:81646518-81646537 Msa1081750:CDS 25.0%
! ATAGTCATTGTTTAATTCTG+AGG + chr7_2:81646852-81646871 None:intergenic 25.0%
! TTCAATGACAATAAAAATGG+AGG - chr7_2:81647055-81647074 Msa1081750:intron 25.0%
!!! GTTGTTCTTTTTGAAACAAT+TGG + chr7_2:81647339-81647358 None:intergenic 25.0%
! TCAAATATTTCAGGTGATGA+AGG - chr7_2:81644943-81644962 Msa1081750:CDS 30.0%
!!! CTTATTTTGCTGTTGATTAG+TGG + chr7_2:81645079-81645098 None:intergenic 30.0%
AGTATAGTTGAATATTTGCC+TGG + chr7_2:81645126-81645145 None:intergenic 30.0%
TATACTCCTAACTATACTCT+AGG - chr7_2:81645192-81645211 Msa1081750:CDS 30.0%
TATTTGCCTAGAGTATAGTT+AGG + chr7_2:81645201-81645220 None:intergenic 30.0%
!!! CTCTAAATGCTTTTTTTGAG+AGG - chr7_2:81645294-81645313 Msa1081750:CDS 30.0%
TTTGGATGAACAGCTTAATT+AGG - chr7_2:81645432-81645451 Msa1081750:CDS 30.0%
! TTAAAGATGAAGGTCTTTTG+GGG + chr7_2:81645665-81645684 None:intergenic 30.0%
! CTTAAAGATGAAGGTCTTTT+GGG + chr7_2:81645666-81645685 None:intergenic 30.0%
! ACTTAAAGATGAAGGTCTTT+TGG + chr7_2:81645667-81645686 None:intergenic 30.0%
AATACTACTTCACAAGAGTA+GGG + chr7_2:81645777-81645796 None:intergenic 30.0%
AAATACTACTTCACAAGAGT+AGG + chr7_2:81645778-81645797 None:intergenic 30.0%
TTATTGTTATGCATATGGCA+AGG - chr7_2:81645867-81645886 Msa1081750:CDS 30.0%
TTTGTTGCCATTAATGTTGT+TGG + chr7_2:81645958-81645977 None:intergenic 30.0%
!!! AATGTTTTTTGATGCCAGTA+AGG + chr7_2:81646164-81646183 None:intergenic 30.0%
! ATTGTGGAACCATATTTTCT+AGG - chr7_2:81646301-81646320 Msa1081750:CDS 30.0%
ATTGCTGTACCTAGAAAATA+TGG + chr7_2:81646313-81646332 None:intergenic 30.0%
CCTGTGTTATACAGAAAAAT+AGG + chr7_2:81646672-81646691 None:intergenic 30.0%
!! CCTATTTTTCTGTATAACAC+AGG - chr7_2:81646669-81646688 Msa1081750:intron 30.0%
ATTGAAGTAATTGAAGCTGT+TGG + chr7_2:81647041-81647060 None:intergenic 30.0%
ATAGAACCAGAAACATGATT+AGG + chr7_2:81647211-81647230 None:intergenic 30.0%
! GGATTAAAGTGTTCATAAGA+GGG + chr7_2:81647259-81647278 None:intergenic 30.0%
! TGGATTAAAGTGTTCATAAG+AGG + chr7_2:81647260-81647279 None:intergenic 30.0%
GATATTGGAATGTTTAGTCA+TGG - chr7_2:81647412-81647431 Msa1081750:intron 30.0%
AGCAATGGAGAAATCAATTT+GGG - chr7_2:81647639-81647658 Msa1081750:CDS 30.0%
AATCTCAATGACTCAAGTTC+AGG - chr7_2:81645024-81645043 Msa1081750:CDS 35.0%
AAGAAGAAAAGAAACCTTCC+TGG - chr7_2:81645096-81645115 Msa1081750:CDS 35.0%
ACTAATCAACACTTCAGTGA+TGG - chr7_2:81645232-81645251 Msa1081750:CDS 35.0%
TTCTCTAATGGTCAAACTCT+TGG - chr7_2:81645374-81645393 Msa1081750:CDS 35.0%
GCTTAAACAATAGCCATGTT+TGG - chr7_2:81645414-81645433 Msa1081750:CDS 35.0%
TTAAGCTGTTCATCCAAACA+TGG + chr7_2:81645430-81645449 None:intergenic 35.0%
AGTCTCACACATACTTATGT+TGG - chr7_2:81645607-81645626 Msa1081750:CDS 35.0%
CATTGGATAGCTTGAATGTT+GGG + chr7_2:81645713-81645732 None:intergenic 35.0%
TCATTGGATAGCTTGAATGT+TGG + chr7_2:81645714-81645733 None:intergenic 35.0%
AACATTCAAGCTATCCAATG+AGG - chr7_2:81645713-81645732 Msa1081750:CDS 35.0%
TATGCAACAAAGGATTTCAG+AGG - chr7_2:81645995-81646014 Msa1081750:intron 35.0%
ATGCAACAAAGGATTTCAGA+GGG - chr7_2:81645996-81646015 Msa1081750:intron 35.0%
TTCGATGTAACTGAAGATTC+TGG + chr7_2:81646023-81646042 None:intergenic 35.0%
AATCTTCAGTTACATCGAAG+AGG - chr7_2:81646024-81646043 Msa1081750:intron 35.0%
ACAATCTTCCATGGAAACTT+AGG - chr7_2:81646049-81646068 Msa1081750:intron 35.0%
!! AAACATTTTAGCCGCAAACA+TGG - chr7_2:81646177-81646196 Msa1081750:intron 35.0%
ATTTCTTCTCACCATGTTTG+CGG + chr7_2:81646191-81646210 None:intergenic 35.0%
GATATGCTGTTCAATCTGAT+TGG - chr7_2:81646235-81646254 Msa1081750:intron 35.0%
AGGGAATACAAATGTGATTG+TGG - chr7_2:81646285-81646304 Msa1081750:intron 35.0%
!!! TTTTCCGCCTTATTTTCACA+CGG - chr7_2:81646344-81646363 Msa1081750:CDS 35.0%
! AGAATGCATGTTTGGAATCA+GGG + chr7_2:81646615-81646634 None:intergenic 35.0%
! TAGAATGCATGTTTGGAATC+AGG + chr7_2:81646616-81646635 None:intergenic 35.0%
GAAAACAACAGAGTAAACCA+AGG - chr7_2:81646740-81646759 Msa1081750:intron 35.0%
TAGAGATAGGATCATGCATT+TGG + chr7_2:81646799-81646818 None:intergenic 35.0%
! GTTTAATTCTGAGGAAGTGT+TGG + chr7_2:81646843-81646862 None:intergenic 35.0%
! GGGATCTTAGTGAGTTTTTA+GGG + chr7_2:81646877-81646896 None:intergenic 35.0%
TTGGTTGATCTGAATGATGT+TGG + chr7_2:81646917-81646936 None:intergenic 35.0%
! GGAATTGGAACTAAGTTGAA+GGG + chr7_2:81647020-81647039 None:intergenic 35.0%
! TGGAATTGGAACTAAGTTGA+AGG + chr7_2:81647021-81647040 None:intergenic 35.0%
GTAATTGAAGCTGTTGGAAT+TGG + chr7_2:81647035-81647054 None:intergenic 35.0%
AAAAATGGAGGACTAATTGC+TGG - chr7_2:81647067-81647086 Msa1081750:intron 35.0%
!!! TGCTGCTTTTTGTAGTAAAG+CGG + chr7_2:81647113-81647132 None:intergenic 35.0%
ACTACAAAAAGCAGCACAAA+TGG - chr7_2:81647117-81647136 Msa1081750:intron 35.0%
CTACAAAAAGCAGCACAAAT+GGG - chr7_2:81647118-81647137 Msa1081750:intron 35.0%
! AACAAAGCTTTTCTGCATCA+TGG + chr7_2:81647173-81647192 None:intergenic 35.0%
!! GAAAAGCTTTGTTAGTAGCA+TGG - chr7_2:81647180-81647199 Msa1081750:intron 35.0%
GTTAGTCCTAATCATGTTTC+TGG - chr7_2:81647202-81647221 Msa1081750:intron 35.0%
! TAAAGTGTTCATAAGAGGGT+TGG + chr7_2:81647255-81647274 None:intergenic 35.0%
TTAGATAATTCATGCTGCAG+TGG + chr7_2:81647280-81647299 None:intergenic 35.0%
GCAGCATGAATTATCTAACA+TGG - chr7_2:81647282-81647301 Msa1081750:intron 35.0%
CATGAATTATCTAACATGGC+TGG - chr7_2:81647286-81647305 Msa1081750:intron 35.0%
TTTGATACATGGAAGTGATG+TGG - chr7_2:81647477-81647496 Msa1081750:intron 35.0%
CAGCAATGGAGAAATCAATT+TGG - chr7_2:81647638-81647657 Msa1081750:CDS 35.0%
TTCTTCTCCATTGTTTCCTG+AGG + chr7_2:81644977-81644996 None:intergenic 40.0%
AGAAGTTAGTAAGCAGCAAG+AGG - chr7_2:81644993-81645012 Msa1081750:CDS 40.0%
GTGTGTGAGATATGCAACAA+AGG - chr7_2:81645985-81646004 Msa1081750:intron 40.0%
GTTCTTTGCCTAAGTTTCCA+TGG + chr7_2:81646060-81646079 None:intergenic 40.0%
GCAAACATGGTGAGAAGAAA+TGG - chr7_2:81646190-81646209 Msa1081750:intron 40.0%
TGCTGTTCAATCTGATTGGA+AGG - chr7_2:81646239-81646258 Msa1081750:intron 40.0%
ACCAGAAAACATGTGGTACT+AGG - chr7_2:81646265-81646284 Msa1081750:intron 40.0%
CCAGAAAACATGTGGTACTA+GGG - chr7_2:81646266-81646285 Msa1081750:intron 40.0%
CAAATCGCCGTGTGAAAATA+AGG + chr7_2:81646354-81646373 None:intergenic 40.0%
GTAAACCAAGGATTAACCAG+TGG - chr7_2:81646752-81646771 Msa1081750:intron 40.0%
!! ATTTGGCTTTGCAAGTTTGG+TGG + chr7_2:81646782-81646801 None:intergenic 40.0%
!! TGCATTTGGCTTTGCAAGTT+TGG + chr7_2:81646785-81646804 None:intergenic 40.0%
! TTTAGAGGCATGGTAGAGAT+AGG + chr7_2:81646812-81646831 None:intergenic 40.0%
!! GTGTTGGAAACAGGTTTTAG+AGG + chr7_2:81646827-81646846 None:intergenic 40.0%
! GGGGATCTTAGTGAGTTTTT+AGG + chr7_2:81646878-81646897 None:intergenic 40.0%
TTGGAGCATGTTCTTGAGTT+GGG + chr7_2:81646898-81646917 None:intergenic 40.0%
AGATCAACCAACAATGCATG+TGG - chr7_2:81646926-81646945 Msa1081750:intron 40.0%
! AAGATGGAGGCAAGGTTTTT+GGG + chr7_2:81646991-81647010 None:intergenic 40.0%
AGTTGAAGGGTTGAAGAAGA+TGG + chr7_2:81647007-81647026 None:intergenic 40.0%
!!! GTTCATAAGAGGGTTGGTTT+TGG + chr7_2:81647249-81647268 None:intergenic 40.0%
GATGAAGAATGTTGCACAAG+AGG - chr7_2:81647438-81647457 Msa1081750:intron 40.0%
GCAACAGTCCATGATTTCTT+GGG - chr7_2:81647499-81647518 Msa1081750:intron 40.0%
GTCCATGATTTCTTGGGAAT+TGG - chr7_2:81647505-81647524 Msa1081750:intron 40.0%
CTCCAATTCCCAAGAAATCA+TGG + chr7_2:81647510-81647529 None:intergenic 40.0%
CATGATTTCTTGGGAATTGG+AGG - chr7_2:81647508-81647527 Msa1081750:intron 40.0%
TGAACCACAGCAACAAAGAT+TGG - chr7_2:81647549-81647568 Msa1081750:CDS 40.0%
!! TGGAAGCATTAAGTCAACAG+AGG - chr7_2:81647569-81647588 Msa1081750:CDS 40.0%
AGCATTAAGTCAACAGAGGT+TGG - chr7_2:81647573-81647592 Msa1081750:CDS 40.0%
TCATCATCATCTTCCACATG+AGG - chr7_2:81647612-81647631 Msa1081750:CDS 40.0%
AGCTTCTCCTCAGGAAACAA+TGG - chr7_2:81644967-81644986 Msa1081750:CDS 45.0%
! AGTGGAACCATTGCTATTGC+AGG + chr7_2:81645061-81645080 None:intergenic 45.0%
GAATATTTGCCTGGAGTTCC+AGG + chr7_2:81645117-81645136 None:intergenic 45.0%
AACAGATCCTACTGCTGAAG+TGG - chr7_2:81645921-81645940 Msa1081750:intron 45.0%
AATGCAACCACTTCAGCAGT+AGG + chr7_2:81645931-81645950 None:intergenic 45.0%
GAAGAGGTCACAATCTTCCA+TGG - chr7_2:81646040-81646059 Msa1081750:intron 45.0%
TAGGCAAAGAACAAGTGCAG+AGG - chr7_2:81646068-81646087 Msa1081750:intron 45.0%
GGATTGTGGTGAACACATGA+TGG + chr7_2:81646123-81646142 None:intergenic 45.0%
AAGGCTCACCAGAAAACATG+TGG - chr7_2:81646258-81646277 Msa1081750:intron 45.0%
! CCCTAGTACCACATGTTTTC+TGG + chr7_2:81646269-81646288 None:intergenic 45.0%
ATCGCCGTGTGAAAATAAGG+CGG + chr7_2:81646351-81646370 None:intergenic 45.0%
TGCATCACAGAATGCTCTGT+GGG + chr7_2:81646711-81646730 None:intergenic 45.0%
ATGCATCACAGAATGCTCTG+TGG + chr7_2:81646712-81646731 None:intergenic 45.0%
!!! GGAAACAGGTTTTAGAGGCA+TGG + chr7_2:81646822-81646841 None:intergenic 45.0%
!! TCTGAGGAAGTGTTGGAAAC+AGG + chr7_2:81646836-81646855 None:intergenic 45.0%
! TGGAGCATGTTCTTGAGTTG+GGG + chr7_2:81646897-81646916 None:intergenic 45.0%
GTTGGAGCATGTTCTTGAGT+TGG + chr7_2:81646899-81646918 None:intergenic 45.0%
TCAACCAACAATGCATGTGG+TGG - chr7_2:81646929-81646948 Msa1081750:intron 45.0%
! ACTAGTGCAGGTGCACTTTT+TGG - chr7_2:81646962-81646981 Msa1081750:intron 45.0%
! GAAGATGGAGGCAAGGTTTT+TGG + chr7_2:81646992-81647011 None:intergenic 45.0%
TGAAGGGTTGAAGAAGATGG+AGG + chr7_2:81647004-81647023 None:intergenic 45.0%
! GTGCTACTGCAAGCAATAGT+GGG - chr7_2:81647140-81647159 Msa1081750:intron 45.0%
! TCTAACATGGCTGGTGTTAG+TGG - chr7_2:81647295-81647314 Msa1081750:intron 45.0%
GGCAACAGTCCATGATTTCT+TGG - chr7_2:81647498-81647517 Msa1081750:intron 45.0%
GCTTCCAATCTTTGTTGCTG+TGG + chr7_2:81647556-81647575 None:intergenic 45.0%
TCCACATGAGGATTCAGCAA+TGG - chr7_2:81647624-81647643 Msa1081750:CDS 45.0%
TCCATTGCTGAATCCTCATG+TGG + chr7_2:81647628-81647647 None:intergenic 45.0%
GATGAAGGAAGCTTCTCCTC+AGG - chr7_2:81644958-81644977 Msa1081750:CDS 50.0%
GACTCAAGTTCAGGTCCATC+TGG - chr7_2:81645033-81645052 Msa1081750:CDS 50.0%
! TTGCTATTGCAGGCACCAGA+TGG + chr7_2:81645051-81645070 None:intergenic 50.0%
TCTGGTGCCTGCAATAGCAA+TGG - chr7_2:81645051-81645070 Msa1081750:CDS 50.0%
AGAAACCTTCCTGGAACTCC+AGG - chr7_2:81645105-81645124 Msa1081750:CDS 50.0%
ATTTGCCTGGAGTTCCAGGA+AGG + chr7_2:81645113-81645132 None:intergenic 50.0%
! TGGCAAGGCACTCCGATTAA+TGG - chr7_2:81645882-81645901 Msa1081750:CDS 50.0%
GGTGAACACATGATGGCTCA+GGG + chr7_2:81646116-81646135 None:intergenic 50.0%
TGGTGAACACATGATGGCTC+AGG + chr7_2:81646117-81646136 None:intergenic 50.0%
CTAATGCACGAGCTGGATTG+TGG + chr7_2:81646137-81646156 None:intergenic 50.0%
CACAATCCAGCTCGTGCATT+AGG - chr7_2:81646135-81646154 Msa1081750:intron 50.0%
CGTGCATTAGGTGACCTTAC+TGG - chr7_2:81646147-81646166 Msa1081750:intron 50.0%
! GCATGCCACTGGTTAATCCT+TGG + chr7_2:81646760-81646779 None:intergenic 50.0%
CATGCCACCACATGCATTGT+TGG + chr7_2:81646936-81646955 None:intergenic 50.0%
GGCATGTTCTCAACTAGTGC+AGG - chr7_2:81646950-81646969 Msa1081750:intron 50.0%
!! AGTGCAGGTGCACTTTTTGG+TGG - chr7_2:81646965-81646984 Msa1081750:intron 50.0%
GGTTGAAGAAGATGGAGGCA+AGG + chr7_2:81646999-81647018 None:intergenic 50.0%
TGGAGGACTAATTGCTGGCA+TGG - chr7_2:81647072-81647091 Msa1081750:intron 50.0%
TGCTGGCATGGCTCAAATGT+CGG - chr7_2:81647084-81647103 Msa1081750:intron 50.0%
!! GGTGCTACTGCAAGCAATAG+TGG - chr7_2:81647139-81647158 Msa1081750:intron 50.0%
! AACATGGCTGGTGTTAGTGG+TGG - chr7_2:81647298-81647317 Msa1081750:intron 50.0%
AGGTCACCTAATGCACGAGC+TGG + chr7_2:81646144-81646163 None:intergenic 55.0%
! CAAGTTTGGTGGCATGCCAC+TGG + chr7_2:81646771-81646790 None:intergenic 55.0%
! ATGGCTGGTGTTAGTGGTGG+AGG - chr7_2:81647301-81647320 Msa1081750:intron 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 81644940 81647669 81644940 ID=Msa1081750;Name=Msa1081750
chr7_2 mRNA 81644940 81647669 81644940 ID=Msa1081750-mRNA-1;Parent=Msa1081750;Name=Msa1081750-mRNA-1;_AED=0.23;_eAED=0.32;_QI=0|0|0|0.66|1|1|3|0|502
chr7_2 exon 81647537 81647669 81647537 ID=Msa1081750-mRNA-1:exon:20971;Parent=Msa1081750-mRNA-1
chr7_2 exon 81646287 81646683 81646287 ID=Msa1081750-mRNA-1:exon:20970;Parent=Msa1081750-mRNA-1
chr7_2 exon 81644940 81645918 81644940 ID=Msa1081750-mRNA-1:exon:20969;Parent=Msa1081750-mRNA-1
chr7_2 CDS 81647537 81647669 81647537 ID=Msa1081750-mRNA-1:cds;Parent=Msa1081750-mRNA-1
chr7_2 CDS 81646287 81646683 81646287 ID=Msa1081750-mRNA-1:cds;Parent=Msa1081750-mRNA-1
chr7_2 CDS 81644940 81645918 81644940 ID=Msa1081750-mRNA-1:cds;Parent=Msa1081750-mRNA-1
Gene Sequence

>Msa1081750

ATGTCAAATATTTCAGGTGATGAAGGAAGCTTCTCCTCAGGAAACAATGGAGAAGAAGTTAGTAAGCAGCAAGAGGTGCAAAATAATCTCAATGACTCAAGTTCAGGTCCATCTGGTGCCTGCAATAGCAATGATCCTACTGCTGAAGTGGTTGCATTATCTCCAACAACATTAATGGCAACAAATAGATTTGTGTGTGAGATATGCAACAAAGGATTTCAGAGGGACCAGAATCTTCAGTTACATCGAAGAGGTCACAATCTTCCATGGAAACTTAGGCAAAGAACAAGTGCAGAGGTGAAGAAAAAAGTGTATGTGTGCCCTGAGCCATCATGTGTTCACCACAATCCAGCTCGTGCATTAGGTGACCTTACTGGCATCAAAAAACATTTTAGCCGCAAACATGGTGAGAAGAAATGGAAATGTGACAAGTGCTCTAAAAGATATGCTGTTCAATCTGATTGGAAGGCTCACCAGAAAACATGTGGTACTAGGGAATACAAATGTGATTGTGGAACCATATTTTCTAGGAGAGACAGTTTTATAACCCACAGAGCATTCTGTGATGCATTAACTGAAGAAAACAACAGAGTAAACCAAGGATTAACCAGTGGCATGCCACCAAACTTGCAAAGCCAAATGCATGATCCTATCTCTACCATGCCTCTAAAACCTGTTTCCAACACTTCCTCAGAATTAAACAATGACTATGACCCTAAAAACTCACTAAGATCCCCAACTCAAGAACATGCTCCAACATCATTCAGATCAACCAACAATGCATGTGGTGGCATGTTCTCAACTAGTGCAGGTGCACTTTTTGGTGGCCCAAAAACCTTGCCTCCATCTTCTTCAACCCTTCAACTTAGTTCCAATTCCAACAGCTTCAATTACTTCAATGACAATAAAAATGGAGGACTAATTGCTGGCATGGCTCAAATGTCGGCAACCGCTTTACTACAAAAAGCAGCACAAATGGGTGCTACTGCAAGCAATAGTGGGAACTCTTCCATGATGCAGAAAAGCTTTGTTAGTAGCATGGTTAGTCCTAATCATGTTTCTGGTTCTATCATGATGCATCATAACCAAAACCAACCCTCTTATGAACACTTTAATCCACTGCAGCATGAATTATCTAACATGGCTGGTGTTAGTGGTGGAGGAGCATTTACTAACCAATTGTTTCAAAAAGAACAACAAGAAATTTCATTACTGTTTGATAACAATACTAATGTGTCTACAATGAATAATGATATTGGAATGTTTAGTCATGGATTGATGAAGAATGTTGCACAAGAGGTTAGTAATTGTTCAAATTTGATACATGGAAGTGATGTGGCAACAGTCCATGATTTCTTGGGAATTGGAGGTTCAAGCTCAAGTTTGCATGAACCACAGCAACAAAGATTGGAAGCATTAAGTCAACAGAGGTTGGAAATTATGAATAATTTTCATCATCATCTTCCACATGAGGATTCAGCAATGGAGAAATCAATTTGGGATGTTTGA
Protein sequence

>Msa1081750

MSNISGDEGSFSSGNNGEEVSKQQEVQNNLNDSSSGPSGACNSNDPTAEVVALSPTTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLRQRTSAEVKKKVYVCPEPSCVHHNPARALGDLTGIKKHFSRKHGEKKWKCDKCSKRYAVQSDWKAHQKTCGTREYKCDCGTIFSRRDSFITHRAFCDALTEENNRVNQGLTSGMPPNLQSQMHDPISTMPLKPVSNTSSELNNDYDPKNSLRSPTQEHAPTSFRSTNNACGGMFSTSAGALFGGPKTLPPSSSTLQLSSNSNSFNYFNDNKNGGLIAGMAQMSATALLQKAAQMGATASNSGNSSMMQKSFVSSMVSPNHVSGSIMMHHNQNQPSYEHFNPLQHELSNMAGVSGGGAFTNQLFQKEQQEISLLFDNNTNVSTMNNDIGMFSHGLMKNVAQEVSNCSNLIHGSDVATVHDFLGIGGSSSSLHEPQQQRLEALSQQRLEIMNNFHHHLPHEDSAMEKSIWDV*