AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1083260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1083260 MtrunA17_Chr7g0270921 97.241 290 6 2 1 289 1 289 0.0 575
Msa1083260 MtrunA17_Chr1g0213571 49.275 69 33 2 214 280 41 109 3.15e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1083260 AT2G46940.1 42.177 294 120 11 1 286 1 252 6.10e-52 171
Msa1083260 AT3G62070.1 45.594 261 90 12 37 289 12 228 6.07e-44 149
Msa1083260 AT4G01670.1 42.160 287 101 8 19 287 4 243 1.97e-42 146
Msa1083260 AT2G31560.2 41.489 94 28 2 214 280 102 195 3.39e-13 67.4
Msa1083260 AT2G31560.1 41.489 94 28 2 214 280 102 195 3.39e-13 67.4
Msa1083260 AT2G31560.3 41.489 94 28 2 214 280 102 195 3.39e-13 67.4
Msa1083260 AT2G43340.1 41.489 94 28 2 214 280 89 182 1.09e-12 65.9
Msa1083260 AT1G05870.4 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.12 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.8 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.1 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.7 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.2 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.10 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.5 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.9 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.3 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.6 42.105 95 27 3 214 280 88 182 3.80e-12 64.3
Msa1083260 AT1G05870.11 42.105 95 27 3 214 280 88 182 3.80e-12 64.3

Find 80 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATAGTTGGGAGAATTTA+TGG 0.098125 7_2:-83546362 Msa1083260:CDS
AGAAGAGTGTATTATATTAA+TGG 0.264708 7_2:+83546933 None:intergenic
TAGGAGTTTGTGTATGGATT+TGG 0.274372 7_2:-83546226 Msa1083260:CDS
GTAATTCACCTATTGCTAAT+TGG 0.279198 7_2:-83546104 Msa1083260:CDS
TTACAACCAGGGTTGTCTTC+TGG 0.287780 7_2:-83546579 Msa1083260:CDS
GTATTAGCTATCAAGAAAAT+TGG 0.318702 7_2:+83545761 None:intergenic
GTTAAAGCTTGTAGAGATCT+TGG 0.319724 7_2:-83546198 Msa1083260:CDS
AGTCGTGCCTTCACATCTCT+TGG 0.356137 7_2:+83545842 None:intergenic
AGGTAGTTGTTATTCGTTAC+CGG 0.356574 7_2:-83546541 Msa1083260:CDS
CTGAAAGCCTTGACAAGTTA+TGG 0.375744 7_2:+83546745 None:intergenic
AAATAGTTGGGAGAATTTAT+GGG 0.384282 7_2:-83546361 Msa1083260:CDS
AGGAAAGTGGTTTGGGAGGT+AGG 0.387981 7_2:-83546428 Msa1083260:CDS
ATCAGCTTCAAAACTTTCAA+TGG 0.388916 7_2:+83546656 None:intergenic
TGGGAATAGGAGTTTGTGTA+TGG 0.391612 7_2:-83546232 Msa1083260:CDS
TGATCAGAGTACTGGGGTTA+TGG 0.393399 7_2:-83546274 Msa1083260:CDS
AGAAACAAAGGAAAGTGGTT+TGG 0.402405 7_2:-83546436 Msa1083260:CDS
ACCACCAAGACGGAGGAGTT+TGG 0.404035 7_2:-83546514 Msa1083260:CDS
GAAACAAAGGAAAGTGGTTT+GGG 0.410000 7_2:-83546435 Msa1083260:CDS
CTGGGGTTATGGTGATTACT+AGG 0.410929 7_2:-83546263 Msa1083260:CDS
TGGGTGTCATGAGCCGTATT+TGG 0.411466 7_2:+83545781 None:intergenic
GTTTGGGAGGTAGGAGAAGA+AGG 0.412318 7_2:-83546419 Msa1083260:CDS
ATCATTCGTTTCTTGTGATA+AGG 0.412876 7_2:+83546984 None:intergenic
AAACTCCTATTCCCACCTTT+AGG 0.424753 7_2:+83546240 None:intergenic
TTCATCTTGAATTGTGATCA+TGG 0.426005 7_2:+83546833 None:intergenic
GGAGAGAGTGATCAGAGTAC+TGG 0.429926 7_2:-83546282 Msa1083260:CDS
TCTGGCTCTGAAAATGAATC+AGG 0.430829 7_2:-83546561 Msa1083260:CDS
ACCCGAAAATCCAGCACCCT+TGG 0.435217 7_2:+83545592 None:intergenic
ATAGCATCTGCCACCAAATA+CGG 0.446123 7_2:-83545794 Msa1083260:CDS
CTAGGCCTAAAGGTGGGAAT+AGG 0.446339 7_2:-83546245 Msa1083260:CDS
AAGAGAATTAGTAGTAGTAG+AGG 0.456429 7_2:+83547012 None:intergenic
GAATGAGAAGTGTGTTTGAA+AGG 0.457694 7_2:-83546386 Msa1083260:CDS
GTAGGAGAAGAAGGAGGAGA+AGG 0.459589 7_2:-83546410 Msa1083260:CDS
TAGAGATCTTGGATTTGAGT+TGG 0.463756 7_2:-83546187 Msa1083260:CDS
GAGATTCGCCAATTAGCAAT+AGG 0.470838 7_2:+83546096 None:intergenic
CTCCGTCTTGGTGGTGTCAC+CGG 0.472824 7_2:+83546522 None:intergenic
GTTTGAAAGGGGAAATAGTT+GGG 0.473057 7_2:-83546373 Msa1083260:CDS
GATGATGATTATATTAATGA+TGG 0.473168 7_2:-83546795 Msa1083260:CDS
TTAGAGAATGGTGTGAATAG+TGG 0.473221 7_2:-83546303 Msa1083260:CDS
TATTAGCTATCAAGAAAATT+GGG 0.476538 7_2:+83545762 None:intergenic
AGAGAATTAGTAGTAGTAGA+GGG 0.477793 7_2:+83547013 None:intergenic
GAGAAAGAGAAATTAGAGAA+TGG 0.488744 7_2:-83546315 Msa1083260:CDS
AATGAGAAGTGTGTTTGAAA+GGG 0.496838 7_2:-83546385 Msa1083260:CDS
CCCGAAAATCCAGCACCCTT+GGG 0.499548 7_2:+83545593 None:intergenic
ACCAAACTCCTCCGTCTTGG+TGG 0.500700 7_2:+83546513 None:intergenic
AAGACGGAGGAGTTTGGTTC+CGG 0.500715 7_2:-83546508 Msa1083260:CDS
GAGAGAGTGATCAGAGTACT+GGG 0.504015 7_2:-83546281 Msa1083260:CDS
TGTTTGAAAGGGGAAATAGT+TGG 0.505309 7_2:-83546374 Msa1083260:CDS
ATTGCTAATTGGCGAATCTC+CGG 0.508950 7_2:-83546093 Msa1083260:CDS
AATTGGCGAATCTCCGGTCC+CGG 0.547996 7_2:-83546087 Msa1083260:intron
GGAACCAAACTCCTCCGTCT+TGG 0.552249 7_2:+83546510 None:intergenic
ATGGTGATTACTAGGCCTAA+AGG 0.555685 7_2:-83546255 Msa1083260:CDS
GAGAATTAGTAGTAGTAGAG+GGG 0.562164 7_2:+83547014 None:intergenic
AGAAAAGAAACAAAGGAAAG+TGG 0.562594 7_2:-83546441 Msa1083260:CDS
AAATTGAGAATTACCGGGAC+CGG 0.564355 7_2:+83546074 None:intergenic
ATGAGAAGTGTGTTTGAAAG+GGG 0.566156 7_2:-83546384 Msa1083260:CDS
CATTCAATAACTGATCATAG+TGG 0.566644 7_2:+83545662 None:intergenic
AAAATCACCATAACTTGTCA+AGG 0.567296 7_2:-83546752 Msa1083260:CDS
AAAATTTGGCATAAATAGAG+TGG 0.569989 7_2:-83547049 Msa1083260:five_prime_UTR
ATGATGATTATATTAATGAT+GGG 0.578381 7_2:-83546794 Msa1083260:CDS
TGAAGCAAGAAAAGAAACAA+AGG 0.581851 7_2:-83546448 Msa1083260:CDS
ACGACTCAAAGTGTGGGCAC+AGG 0.585079 7_2:-83545825 Msa1083260:CDS
ACAAAGGAAAGTGGTTTGGG+AGG 0.586780 7_2:-83546432 Msa1083260:CDS
TGATGATCCAAGAGATGTGA+AGG 0.588464 7_2:-83545849 Msa1083260:CDS
TGGGAGGTAGGAGAAGAAGG+AGG 0.588937 7_2:-83546416 Msa1083260:CDS
TGAAGGCACGACTCAAAGTG+TGG 0.596523 7_2:-83545832 Msa1083260:CDS
GATGATCAGTTATTACAACC+AGG 0.603207 7_2:-83546591 Msa1083260:CDS
GTGTCATGAGCCGTATTTGG+TGG 0.611631 7_2:+83545784 None:intergenic
GTGATTACTAGGCCTAAAGG+TGG 0.627025 7_2:-83546252 Msa1083260:CDS
TCAGAGCCAGAAGACAACCC+TGG 0.636829 7_2:+83546573 None:intergenic
ACACTTGATACTAGCAGTGG+TGG 0.641295 7_2:-83546126 Msa1083260:CDS
ATGATCAGTTATTACAACCA+GGG 0.642055 7_2:-83546590 Msa1083260:CDS
TACCGGTGACACCACCAAGA+CGG 0.643200 7_2:-83546524 Msa1083260:CDS
GAAGGCACGACTCAAAGTGT+GGG 0.668787 7_2:-83545831 Msa1083260:CDS
TGATTACTAGGCCTAAAGGT+GGG 0.670590 7_2:-83546251 Msa1083260:CDS
GGACAAGTTTGACATACACA+TGG 0.672211 7_2:+83546677 None:intergenic
TCAACACTTGATACTAGCAG+TGG 0.690895 7_2:-83546129 Msa1083260:CDS
CGGTGACACCACCAAGACGG+AGG 0.698426 7_2:-83546521 Msa1083260:CDS
TATACACTTCCACTGTACAG+AGG 0.707736 7_2:+83545552 None:intergenic
GATGGTGGTGATGAAGCAGA+TGG 0.717318 7_2:-83546636 Msa1083260:CDS
AGAGAGTGATCAGAGTACTG+GGG 0.774446 7_2:-83546280 Msa1083260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTGTTTTATATTATGT+AGG - chr7_2:83546679-83546698 Msa1083260:CDS 10.0%
!!! TTTCATTGTATTTTTTTTTT+TGG - chr7_2:83546914-83546933 Msa1083260:five_prime_UTR 10.0%
!!! ATTTTTGTTTTATATTATGT+AGG - chr7_2:83546679-83546698 Msa1083260:CDS 10.0%
!!! TTTCATTGTATTTTTTTTTT+TGG - chr7_2:83546914-83546933 Msa1083260:five_prime_UTR 10.0%
!! ATGATGATTATATTAATGAT+GGG - chr7_2:83545757-83545776 Msa1083260:three_prime_UTR 15.0%
!! ATAAACATTATAAGATTGTT+TGG - chr7_2:83546548-83546567 Msa1083260:CDS 15.0%
!! ATGATGATTATATTAATGAT+GGG - chr7_2:83545757-83545776 Msa1083260:three_prime_UTR 15.0%
!! ATAAACATTATAAGATTGTT+TGG - chr7_2:83546548-83546567 Msa1083260:CDS 15.0%
!!! AGAAGAGTGTATTATATTAA+TGG + chr7_2:83545621-83545640 None:intergenic 20.0%
!!! AAGAACGTACTATTTTATTT+TGG + chr7_2:83545673-83545692 None:intergenic 20.0%
!!! GTACGTTCTTTTTTTATATA+TGG - chr7_2:83545683-83545702 Msa1083260:three_prime_UTR 20.0%
!! GATGATGATTATATTAATGA+TGG - chr7_2:83545756-83545775 Msa1083260:three_prime_UTR 20.0%
!! AAAACAATTTATGACATGTT+CGG - chr7_2:83546582-83546601 Msa1083260:CDS 20.0%
!! TATTAGCTATCAAGAAAATT+GGG + chr7_2:83546792-83546811 None:intergenic 20.0%
!!! AGAAGAGTGTATTATATTAA+TGG + chr7_2:83545621-83545640 None:intergenic 20.0%
!!! AAGAACGTACTATTTTATTT+TGG + chr7_2:83545673-83545692 None:intergenic 20.0%
!!! GTACGTTCTTTTTTTATATA+TGG - chr7_2:83545683-83545702 Msa1083260:three_prime_UTR 20.0%
!! GATGATGATTATATTAATGA+TGG - chr7_2:83545756-83545775 Msa1083260:three_prime_UTR 20.0%
!! AAAACAATTTATGACATGTT+CGG - chr7_2:83546582-83546601 Msa1083260:CDS 20.0%
!! TATTAGCTATCAAGAAAATT+GGG + chr7_2:83546792-83546811 None:intergenic 20.0%
! AAAATTTGGCATAAATAGAG+TGG - chr7_2:83545502-83545521 Msa1083260:three_prime_UTR 25.0%
! AAATAGTTGGGAGAATTTAT+GGG - chr7_2:83546190-83546209 Msa1083260:CDS 25.0%
! GTATTAGCTATCAAGAAAAT+TGG + chr7_2:83546793-83546812 None:intergenic 25.0%
!!! AATACCTACTTCCTTTTTTT+TGG - chr7_2:83546808-83546827 Msa1083260:CDS 25.0%
!!! TTGTCATAAATTTTTTCCCA+AGG - chr7_2:83546942-83546961 Msa1083260:five_prime_UTR 25.0%
!!! TGTCATAAATTTTTTCCCAA+GGG - chr7_2:83546943-83546962 Msa1083260:five_prime_UTR 25.0%
!!! TTTTTGTTTAAACTGCTTAC+TGG - chr7_2:83547029-83547048 Msa1083260:five_prime_UTR 25.0%
! AAAATTTGGCATAAATAGAG+TGG - chr7_2:83545502-83545521 Msa1083260:three_prime_UTR 25.0%
! AAATAGTTGGGAGAATTTAT+GGG - chr7_2:83546190-83546209 Msa1083260:CDS 25.0%
! GTATTAGCTATCAAGAAAAT+TGG + chr7_2:83546793-83546812 None:intergenic 25.0%
!!! AATACCTACTTCCTTTTTTT+TGG - chr7_2:83546808-83546827 Msa1083260:CDS 25.0%
!!! TTGTCATAAATTTTTTCCCA+AGG - chr7_2:83546942-83546961 Msa1083260:five_prime_UTR 25.0%
!!! TGTCATAAATTTTTTCCCAA+GGG - chr7_2:83546943-83546962 Msa1083260:five_prime_UTR 25.0%
!!! TTTTTGTTTAAACTGCTTAC+TGG - chr7_2:83547029-83547048 Msa1083260:five_prime_UTR 25.0%
AGAGAATTAGTAGTAGTAGA+GGG + chr7_2:83545541-83545560 None:intergenic 30.0%
AAGAGAATTAGTAGTAGTAG+AGG + chr7_2:83545542-83545561 None:intergenic 30.0%
ATCATTCGTTTCTTGTGATA+AGG + chr7_2:83545570-83545589 None:intergenic 30.0%
TTCATCTTGAATTGTGATCA+TGG + chr7_2:83545721-83545740 None:intergenic 30.0%
! AAAATCACCATAACTTGTCA+AGG - chr7_2:83545799-83545818 Msa1083260:CDS 30.0%
ATCAGCTTCAAAACTTTCAA+TGG + chr7_2:83545898-83545917 None:intergenic 30.0%
!!! ATTGAAAGTTTTGAAGCTGA+TGG - chr7_2:83545897-83545916 Msa1083260:intron 30.0%
ATGATCAGTTATTACAACCA+GGG - chr7_2:83545961-83545980 Msa1083260:intron 30.0%
TGAAGCAAGAAAAGAAACAA+AGG - chr7_2:83546103-83546122 Msa1083260:CDS 30.0%
AGAAAAGAAACAAAGGAAAG+TGG - chr7_2:83546110-83546129 Msa1083260:CDS 30.0%
! AATGAGAAGTGTGTTTGAAA+GGG - chr7_2:83546166-83546185 Msa1083260:CDS 30.0%
GAAATAGTTGGGAGAATTTA+TGG - chr7_2:83546189-83546208 Msa1083260:CDS 30.0%
GAGAAAGAGAAATTAGAGAA+TGG - chr7_2:83546236-83546255 Msa1083260:CDS 30.0%
GTAATTCACCTATTGCTAAT+TGG - chr7_2:83546447-83546466 Msa1083260:CDS 30.0%
AACCAAAATTGAGAATTACC+GGG + chr7_2:83546485-83546504 None:intergenic 30.0%
GAACCAAAATTGAGAATTAC+CGG + chr7_2:83546486-83546505 None:intergenic 30.0%
! TTTGGTTCATATTTCCGATT+AGG - chr7_2:83546498-83546517 Msa1083260:CDS 30.0%
TAAGCGCGTATCATATATTA+TGG + chr7_2:83546626-83546645 None:intergenic 30.0%
CATTCAATAACTGATCATAG+TGG + chr7_2:83546892-83546911 None:intergenic 30.0%
AGAGAATTAGTAGTAGTAGA+GGG + chr7_2:83545541-83545560 None:intergenic 30.0%
AAGAGAATTAGTAGTAGTAG+AGG + chr7_2:83545542-83545561 None:intergenic 30.0%
ATCATTCGTTTCTTGTGATA+AGG + chr7_2:83545570-83545589 None:intergenic 30.0%
TTCATCTTGAATTGTGATCA+TGG + chr7_2:83545721-83545740 None:intergenic 30.0%
! AAAATCACCATAACTTGTCA+AGG - chr7_2:83545799-83545818 Msa1083260:CDS 30.0%
ATCAGCTTCAAAACTTTCAA+TGG + chr7_2:83545898-83545917 None:intergenic 30.0%
!!! ATTGAAAGTTTTGAAGCTGA+TGG - chr7_2:83545897-83545916 Msa1083260:intron 30.0%
ATGATCAGTTATTACAACCA+GGG - chr7_2:83545961-83545980 Msa1083260:intron 30.0%
TGAAGCAAGAAAAGAAACAA+AGG - chr7_2:83546103-83546122 Msa1083260:CDS 30.0%
AGAAAAGAAACAAAGGAAAG+TGG - chr7_2:83546110-83546129 Msa1083260:CDS 30.0%
! AATGAGAAGTGTGTTTGAAA+GGG - chr7_2:83546166-83546185 Msa1083260:CDS 30.0%
GAAATAGTTGGGAGAATTTA+TGG - chr7_2:83546189-83546208 Msa1083260:CDS 30.0%
GAGAAAGAGAAATTAGAGAA+TGG - chr7_2:83546236-83546255 Msa1083260:CDS 30.0%
GTAATTCACCTATTGCTAAT+TGG - chr7_2:83546447-83546466 Msa1083260:CDS 30.0%
AACCAAAATTGAGAATTACC+GGG + chr7_2:83546485-83546504 None:intergenic 30.0%
GAACCAAAATTGAGAATTAC+CGG + chr7_2:83546486-83546505 None:intergenic 30.0%
! TTTGGTTCATATTTCCGATT+AGG - chr7_2:83546498-83546517 Msa1083260:CDS 30.0%
TAAGCGCGTATCATATATTA+TGG + chr7_2:83546626-83546645 None:intergenic 30.0%
CATTCAATAACTGATCATAG+TGG + chr7_2:83546892-83546911 None:intergenic 30.0%
GAGAATTAGTAGTAGTAGAG+GGG + chr7_2:83545540-83545559 None:intergenic 35.0%
GATGATCAGTTATTACAACC+AGG - chr7_2:83545960-83545979 Msa1083260:intron 35.0%
! AGGTAGTTGTTATTCGTTAC+CGG - chr7_2:83546010-83546029 Msa1083260:intron 35.0%
AGAAACAAAGGAAAGTGGTT+TGG - chr7_2:83546115-83546134 Msa1083260:CDS 35.0%
GAAACAAAGGAAAGTGGTTT+GGG - chr7_2:83546116-83546135 Msa1083260:CDS 35.0%
! GAATGAGAAGTGTGTTTGAA+AGG - chr7_2:83546165-83546184 Msa1083260:CDS 35.0%
! ATGAGAAGTGTGTTTGAAAG+GGG - chr7_2:83546167-83546186 Msa1083260:CDS 35.0%
TGTTTGAAAGGGGAAATAGT+TGG - chr7_2:83546177-83546196 Msa1083260:CDS 35.0%
GTTTGAAAGGGGAAATAGTT+GGG - chr7_2:83546178-83546197 Msa1083260:CDS 35.0%
TTAGAGAATGGTGTGAATAG+TGG - chr7_2:83546248-83546267 Msa1083260:CDS 35.0%
TAGGAGTTTGTGTATGGATT+TGG - chr7_2:83546325-83546344 Msa1083260:CDS 35.0%
GTTAAAGCTTGTAGAGATCT+TGG - chr7_2:83546353-83546372 Msa1083260:CDS 35.0%
TAGAGATCTTGGATTTGAGT+TGG - chr7_2:83546364-83546383 Msa1083260:CDS 35.0%
! CTCGAATAAGTTGACCTAAT+CGG + chr7_2:83546515-83546534 None:intergenic 35.0%
ATCGCCAAAAAAAAGGAAGT+AGG + chr7_2:83546815-83546834 None:intergenic 35.0%
TGTCTCAATCGCCAAAAAAA+AGG + chr7_2:83546822-83546841 None:intergenic 35.0%
GAGAATTAGTAGTAGTAGAG+GGG + chr7_2:83545540-83545559 None:intergenic 35.0%
GATGATCAGTTATTACAACC+AGG - chr7_2:83545960-83545979 Msa1083260:intron 35.0%
! AGGTAGTTGTTATTCGTTAC+CGG - chr7_2:83546010-83546029 Msa1083260:intron 35.0%
AGAAACAAAGGAAAGTGGTT+TGG - chr7_2:83546115-83546134 Msa1083260:CDS 35.0%
GAAACAAAGGAAAGTGGTTT+GGG - chr7_2:83546116-83546135 Msa1083260:CDS 35.0%
! GAATGAGAAGTGTGTTTGAA+AGG - chr7_2:83546165-83546184 Msa1083260:CDS 35.0%
! ATGAGAAGTGTGTTTGAAAG+GGG - chr7_2:83546167-83546186 Msa1083260:CDS 35.0%
TGTTTGAAAGGGGAAATAGT+TGG - chr7_2:83546177-83546196 Msa1083260:CDS 35.0%
GTTTGAAAGGGGAAATAGTT+GGG - chr7_2:83546178-83546197 Msa1083260:CDS 35.0%
TTAGAGAATGGTGTGAATAG+TGG - chr7_2:83546248-83546267 Msa1083260:CDS 35.0%
TAGGAGTTTGTGTATGGATT+TGG - chr7_2:83546325-83546344 Msa1083260:CDS 35.0%
GTTAAAGCTTGTAGAGATCT+TGG - chr7_2:83546353-83546372 Msa1083260:CDS 35.0%
TAGAGATCTTGGATTTGAGT+TGG - chr7_2:83546364-83546383 Msa1083260:CDS 35.0%
! CTCGAATAAGTTGACCTAAT+CGG + chr7_2:83546515-83546534 None:intergenic 35.0%
ATCGCCAAAAAAAAGGAAGT+AGG + chr7_2:83546815-83546834 None:intergenic 35.0%
TGTCTCAATCGCCAAAAAAA+AGG + chr7_2:83546822-83546841 None:intergenic 35.0%
CTGAAAGCCTTGACAAGTTA+TGG + chr7_2:83545809-83545828 None:intergenic 40.0%
GGACAAGTTTGACATACACA+TGG + chr7_2:83545877-83545896 None:intergenic 40.0%
!!! GAAAGTTTTGAAGCTGATGG+TGG - chr7_2:83545900-83545919 Msa1083260:intron 40.0%
! GATGGTGAATTTTCTGCTGA+TGG - chr7_2:83545933-83545952 Msa1083260:intron 40.0%
!! TCTGGCTCTGAAAATGAATC+AGG - chr7_2:83545990-83546009 Msa1083260:intron 40.0%
TTCACTCCAAAAACACTCAC+CGG + chr7_2:83546065-83546084 None:intergenic 40.0%
ATGGTGATTACTAGGCCTAA+AGG - chr7_2:83546296-83546315 Msa1083260:CDS 40.0%
TGATTACTAGGCCTAAAGGT+GGG - chr7_2:83546300-83546319 Msa1083260:CDS 40.0%
AAACTCCTATTCCCACCTTT+AGG + chr7_2:83546314-83546333 None:intergenic 40.0%
TGGGAATAGGAGTTTGTGTA+TGG - chr7_2:83546319-83546338 Msa1083260:CDS 40.0%
TCAACACTTGATACTAGCAG+TGG - chr7_2:83546422-83546441 Msa1083260:CDS 40.0%
GAGATTCGCCAATTAGCAAT+AGG + chr7_2:83546458-83546477 None:intergenic 40.0%
ATTGCTAATTGGCGAATCTC+CGG - chr7_2:83546458-83546477 Msa1083260:CDS 40.0%
AAATTGAGAATTACCGGGAC+CGG + chr7_2:83546480-83546499 None:intergenic 40.0%
! GTCCCGGTAATTCTCAATTT+TGG - chr7_2:83546480-83546499 Msa1083260:CDS 40.0%
TGATGATCCAAGAGATGTGA+AGG - chr7_2:83546702-83546721 Msa1083260:CDS 40.0%
ATAGCATCTGCCACCAAATA+CGG - chr7_2:83546757-83546776 Msa1083260:CDS 40.0%
!!! AAATTTTTTCCCAAGGGTGC+TGG - chr7_2:83546949-83546968 Msa1083260:five_prime_UTR 40.0%
TATACACTTCCACTGTACAG+AGG + chr7_2:83547002-83547021 None:intergenic 40.0%
CTGAAAGCCTTGACAAGTTA+TGG + chr7_2:83545809-83545828 None:intergenic 40.0%
GGACAAGTTTGACATACACA+TGG + chr7_2:83545877-83545896 None:intergenic 40.0%
!!! GAAAGTTTTGAAGCTGATGG+TGG - chr7_2:83545900-83545919 Msa1083260:intron 40.0%
! GATGGTGAATTTTCTGCTGA+TGG - chr7_2:83545933-83545952 Msa1083260:intron 40.0%
!! TCTGGCTCTGAAAATGAATC+AGG - chr7_2:83545990-83546009 Msa1083260:intron 40.0%
TTCACTCCAAAAACACTCAC+CGG + chr7_2:83546065-83546084 None:intergenic 40.0%
ATGGTGATTACTAGGCCTAA+AGG - chr7_2:83546296-83546315 Msa1083260:CDS 40.0%
TGATTACTAGGCCTAAAGGT+GGG - chr7_2:83546300-83546319 Msa1083260:CDS 40.0%
AAACTCCTATTCCCACCTTT+AGG + chr7_2:83546314-83546333 None:intergenic 40.0%
TGGGAATAGGAGTTTGTGTA+TGG - chr7_2:83546319-83546338 Msa1083260:CDS 40.0%
TCAACACTTGATACTAGCAG+TGG - chr7_2:83546422-83546441 Msa1083260:CDS 40.0%
GAGATTCGCCAATTAGCAAT+AGG + chr7_2:83546458-83546477 None:intergenic 40.0%
ATTGCTAATTGGCGAATCTC+CGG - chr7_2:83546458-83546477 Msa1083260:CDS 40.0%
AAATTGAGAATTACCGGGAC+CGG + chr7_2:83546480-83546499 None:intergenic 40.0%
! GTCCCGGTAATTCTCAATTT+TGG - chr7_2:83546480-83546499 Msa1083260:CDS 40.0%
TGATGATCCAAGAGATGTGA+AGG - chr7_2:83546702-83546721 Msa1083260:CDS 40.0%
ATAGCATCTGCCACCAAATA+CGG - chr7_2:83546757-83546776 Msa1083260:CDS 40.0%
!!! AAATTTTTTCCCAAGGGTGC+TGG - chr7_2:83546949-83546968 Msa1083260:five_prime_UTR 40.0%
TATACACTTCCACTGTACAG+AGG + chr7_2:83547002-83547021 None:intergenic 40.0%
TTACAACCAGGGTTGTCTTC+TGG - chr7_2:83545972-83545991 Msa1083260:intron 45.0%
! TTGGTTCCGGTGAGTGTTTT+TGG - chr7_2:83546056-83546075 Msa1083260:intron 45.0%
! ACAAAGGAAAGTGGTTTGGG+AGG - chr7_2:83546119-83546138 Msa1083260:CDS 45.0%
! GAGAGAGTGATCAGAGTACT+GGG - chr7_2:83546270-83546289 Msa1083260:CDS 45.0%
!! AGAGAGTGATCAGAGTACTG+GGG - chr7_2:83546271-83546290 Msa1083260:CDS 45.0%
!! TGATCAGAGTACTGGGGTTA+TGG - chr7_2:83546277-83546296 Msa1083260:CDS 45.0%
CTGGGGTTATGGTGATTACT+AGG - chr7_2:83546288-83546307 Msa1083260:CDS 45.0%
GTGATTACTAGGCCTAAAGG+TGG - chr7_2:83546299-83546318 Msa1083260:CDS 45.0%
ACACTTGATACTAGCAGTGG+TGG - chr7_2:83546425-83546444 Msa1083260:CDS 45.0%
!!! CTGGATTTTCGGGTATGTGA+TGG - chr7_2:83546968-83546987 Msa1083260:five_prime_UTR 45.0%
! GTAGCTTTTCCTCTGTACAG+TGG - chr7_2:83546990-83547009 Msa1083260:five_prime_UTR 45.0%
TTACAACCAGGGTTGTCTTC+TGG - chr7_2:83545972-83545991 Msa1083260:intron 45.0%
! TTGGTTCCGGTGAGTGTTTT+TGG - chr7_2:83546056-83546075 Msa1083260:intron 45.0%
! ACAAAGGAAAGTGGTTTGGG+AGG - chr7_2:83546119-83546138 Msa1083260:CDS 45.0%
! GAGAGAGTGATCAGAGTACT+GGG - chr7_2:83546270-83546289 Msa1083260:CDS 45.0%
!! AGAGAGTGATCAGAGTACTG+GGG - chr7_2:83546271-83546290 Msa1083260:CDS 45.0%
!! TGATCAGAGTACTGGGGTTA+TGG - chr7_2:83546277-83546296 Msa1083260:CDS 45.0%
CTGGGGTTATGGTGATTACT+AGG - chr7_2:83546288-83546307 Msa1083260:CDS 45.0%
GTGATTACTAGGCCTAAAGG+TGG - chr7_2:83546299-83546318 Msa1083260:CDS 45.0%
ACACTTGATACTAGCAGTGG+TGG - chr7_2:83546425-83546444 Msa1083260:CDS 45.0%
!!! CTGGATTTTCGGGTATGTGA+TGG - chr7_2:83546968-83546987 Msa1083260:five_prime_UTR 45.0%
! GTAGCTTTTCCTCTGTACAG+TGG - chr7_2:83546990-83547009 Msa1083260:five_prime_UTR 45.0%
! GATGGTGGTGATGAAGCAGA+TGG - chr7_2:83545915-83545934 Msa1083260:intron 50.0%
! AAGACGGAGGAGTTTGGTTC+CGG - chr7_2:83546043-83546062 Msa1083260:intron 50.0%
! AGGAAAGTGGTTTGGGAGGT+AGG - chr7_2:83546123-83546142 Msa1083260:CDS 50.0%
GTTTGGGAGGTAGGAGAAGA+AGG - chr7_2:83546132-83546151 Msa1083260:CDS 50.0%
GTAGGAGAAGAAGGAGGAGA+AGG - chr7_2:83546141-83546160 Msa1083260:CDS 50.0%
GGAGAGAGTGATCAGAGTAC+TGG - chr7_2:83546269-83546288 Msa1083260:CDS 50.0%
CTAGGCCTAAAGGTGGGAAT+AGG - chr7_2:83546306-83546325 Msa1083260:CDS 50.0%
AGTCGTGCCTTCACATCTCT+TGG + chr7_2:83546712-83546731 None:intergenic 50.0%
! TGAAGGCACGACTCAAAGTG+TGG - chr7_2:83546719-83546738 Msa1083260:CDS 50.0%
! GAAGGCACGACTCAAAGTGT+GGG - chr7_2:83546720-83546739 Msa1083260:CDS 50.0%
GTGTCATGAGCCGTATTTGG+TGG + chr7_2:83546770-83546789 None:intergenic 50.0%
! TGGGTGTCATGAGCCGTATT+TGG + chr7_2:83546773-83546792 None:intergenic 50.0%
!!! TCCCAAGGGTGCTGGATTTT+CGG - chr7_2:83546957-83546976 Msa1083260:five_prime_UTR 50.0%
! GATGGTGGTGATGAAGCAGA+TGG - chr7_2:83545915-83545934 Msa1083260:intron 50.0%
! AAGACGGAGGAGTTTGGTTC+CGG - chr7_2:83546043-83546062 Msa1083260:intron 50.0%
! AGGAAAGTGGTTTGGGAGGT+AGG - chr7_2:83546123-83546142 Msa1083260:CDS 50.0%
GTTTGGGAGGTAGGAGAAGA+AGG - chr7_2:83546132-83546151 Msa1083260:CDS 50.0%
GTAGGAGAAGAAGGAGGAGA+AGG - chr7_2:83546141-83546160 Msa1083260:CDS 50.0%
GGAGAGAGTGATCAGAGTAC+TGG - chr7_2:83546269-83546288 Msa1083260:CDS 50.0%
CTAGGCCTAAAGGTGGGAAT+AGG - chr7_2:83546306-83546325 Msa1083260:CDS 50.0%
AGTCGTGCCTTCACATCTCT+TGG + chr7_2:83546712-83546731 None:intergenic 50.0%
! TGAAGGCACGACTCAAAGTG+TGG - chr7_2:83546719-83546738 Msa1083260:CDS 50.0%
! GAAGGCACGACTCAAAGTGT+GGG - chr7_2:83546720-83546739 Msa1083260:CDS 50.0%
GTGTCATGAGCCGTATTTGG+TGG + chr7_2:83546770-83546789 None:intergenic 50.0%
! TGGGTGTCATGAGCCGTATT+TGG + chr7_2:83546773-83546792 None:intergenic 50.0%
!!! TCCCAAGGGTGCTGGATTTT+CGG - chr7_2:83546957-83546976 Msa1083260:five_prime_UTR 50.0%
TCAGAGCCAGAAGACAACCC+TGG + chr7_2:83545981-83546000 None:intergenic 55.0%
TACCGGTGACACCACCAAGA+CGG - chr7_2:83546027-83546046 Msa1083260:intron 55.0%
ACCACCAAGACGGAGGAGTT+TGG - chr7_2:83546037-83546056 Msa1083260:intron 55.0%
ACCAAACTCCTCCGTCTTGG+TGG + chr7_2:83546041-83546060 None:intergenic 55.0%
GGAACCAAACTCCTCCGTCT+TGG + chr7_2:83546044-83546063 None:intergenic 55.0%
TGGGAGGTAGGAGAAGAAGG+AGG - chr7_2:83546135-83546154 Msa1083260:CDS 55.0%
AATTGGCGAATCTCCGGTCC+CGG - chr7_2:83546464-83546483 Msa1083260:CDS 55.0%
ACGACTCAAAGTGTGGGCAC+AGG - chr7_2:83546726-83546745 Msa1083260:CDS 55.0%
CCCGAAAATCCAGCACCCTT+GGG + chr7_2:83546961-83546980 None:intergenic 55.0%
!!! CCCAAGGGTGCTGGATTTTC+GGG - chr7_2:83546958-83546977 Msa1083260:five_prime_UTR 55.0%
ACCCGAAAATCCAGCACCCT+TGG + chr7_2:83546962-83546981 None:intergenic 55.0%
TCAGAGCCAGAAGACAACCC+TGG + chr7_2:83545981-83546000 None:intergenic 55.0%
TACCGGTGACACCACCAAGA+CGG - chr7_2:83546027-83546046 Msa1083260:intron 55.0%
ACCACCAAGACGGAGGAGTT+TGG - chr7_2:83546037-83546056 Msa1083260:intron 55.0%
ACCAAACTCCTCCGTCTTGG+TGG + chr7_2:83546041-83546060 None:intergenic 55.0%
GGAACCAAACTCCTCCGTCT+TGG + chr7_2:83546044-83546063 None:intergenic 55.0%
TGGGAGGTAGGAGAAGAAGG+AGG - chr7_2:83546135-83546154 Msa1083260:CDS 55.0%
AATTGGCGAATCTCCGGTCC+CGG - chr7_2:83546464-83546483 Msa1083260:CDS 55.0%
ACGACTCAAAGTGTGGGCAC+AGG - chr7_2:83546726-83546745 Msa1083260:CDS 55.0%
CCCGAAAATCCAGCACCCTT+GGG + chr7_2:83546961-83546980 None:intergenic 55.0%
!!! CCCAAGGGTGCTGGATTTTC+GGG - chr7_2:83546958-83546977 Msa1083260:five_prime_UTR 55.0%
ACCCGAAAATCCAGCACCCT+TGG + chr7_2:83546962-83546981 None:intergenic 55.0%
!! CTCCGTCTTGGTGGTGTCAC+CGG + chr7_2:83546032-83546051 None:intergenic 60.0%
!! CTCCGTCTTGGTGGTGTCAC+CGG + chr7_2:83546032-83546051 None:intergenic 60.0%
CGGTGACACCACCAAGACGG+AGG - chr7_2:83546030-83546049 Msa1083260:intron 65.0%
CGGTGACACCACCAAGACGG+AGG - chr7_2:83546030-83546049 Msa1083260:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 83545501 83547072 83545501 ID=Msa1083260;Name=Msa1083260
chr7_2 mRNA 83545501 83547072 83545501 ID=Msa1083260-mRNA-1;Parent=Msa1083260;Name=Msa1083260-mRNA-1;_AED=0.05;_eAED=0.05;_QI=201|1|1|1|1|1|2|286|289
chr7_2 exon 83545501 83545872 83545501 ID=Msa1083260-mRNA-1:exon:21972;Parent=Msa1083260-mRNA-1
chr7_2 exon 83546088 83547072 83546088 ID=Msa1083260-mRNA-1:exon:21971;Parent=Msa1083260-mRNA-1
chr7_2 five_prime_UTR 83546872 83547072 83546872 ID=Msa1083260-mRNA-1:five_prime_utr;Parent=Msa1083260-mRNA-1
chr7_2 CDS 83546088 83546871 83546088 ID=Msa1083260-mRNA-1:cds;Parent=Msa1083260-mRNA-1
chr7_2 CDS 83545787 83545872 83545787 ID=Msa1083260-mRNA-1:cds;Parent=Msa1083260-mRNA-1
chr7_2 three_prime_UTR 83545501 83545786 83545501 ID=Msa1083260-mRNA-1:three_prime_utr;Parent=Msa1083260-mRNA-1
Gene Sequence

>Msa1083260

ATGGCTTCAGATGAGACCATGATCACAATTCAAGATGAAGACTTTGAAGAAGATGATGATGATTATATTAATGATGGGTTTGATCATCATCATAATCAAAATCACCATAACTTGTCAAGGCTTTCAGTTTGTACAAACAGCACTATATGTGATGATGTTGATAATCTAGCTTCCATGTGTATGTCAAACTTGTCCATTGAAAGTTTTGAAGCTGATGGTGGTGATGAAGCAGATGGTGAATTTTCTGCTGATGGAAAAGATGATCAGTTATTACAACCAGGGTTGTCTTCTGGCTCTGAAAATGAATCAGGTAGTTGTTATTCGTTACCGGTGACACCACCAAGACGGAGGAGTTTGGTTCCGGTGAGTGTTTTTGGAGTGAAAGATTATGCAAGTGAGAATGAAGCAAGAAAAGAAACAAAGGAAAGTGGTTTGGGAGGTAGGAGAAGAAGGAGGAGAAGGAGAATGAGAAGTGTGTTTGAAAGGGGAAATAGTTGGGAGAATTTATGGGATCAGAAGAAGAAAATGATGAAAGAGAAAGAGAAATTAGAGAATGGTGTGAATAGTGGAGAGAGTGATCAGAGTACTGGGGTTATGGTGATTACTAGGCCTAAAGGTGGGAATAGGAGTTTGTGTATGGATTTGGAAGAAGTTAAAGCTTGTAGAGATCTTGGATTTGAGTTGGAACATGAAAGAATTTCTGCTGTTTCTTTCTCTAATTCAACACTTGATACTAGCAGTGGTGGTAATTCACCTATTGCTAATTGGCGAATCTCCGGTCCCGGTGATGATCCAAGAGATGTGAAGGCACGACTCAAAGTGTGGGCACAGGCAGTAGCAATAGCATCTGCCACCAAATACGGCTCATGA
Protein sequence

>Msa1083260

MASDETMITIQDEDFEEDDDDYINDGFDHHHNQNHHNLSRLSVCTNSTICDDVDNLASMCMSNLSIESFEADGGDEADGEFSADGKDDQLLQPGLSSGSENESGSCYSLPVTPPRRRSLVPVSVFGVKDYASENEARKETKESGLGGRRRRRRRRMRSVFERGNSWENLWDQKKKMMKEKEKLENGVNSGESDQSTGVMVITRPKGGNRSLCMDLEEVKACRDLGFELEHERISAVSFSNSTLDTSSGGNSPIANWRISGPGDDPRDVKARLKVWAQAVAIASATKYGS*