AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1096330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1096330 MtrunA17_Chr7g0222171 86.607 336 19 3 1 311 1 335 0.0 585
Msa1096330 MtrunA17_Chr7g0221791 83.631 336 29 3 1 311 1 335 0.0 573
Msa1096330 MtrunA17_Chr7g0222121 75.946 370 29 4 1 311 1 369 0.0 554
Msa1096330 MtrunA17_Chr1g0204651 56.716 335 115 7 4 308 5 339 2.14e-126 364
Msa1096330 MtrunA17_Chr7g0274071 53.799 329 125 5 7 308 16 344 8.14e-123 355
Msa1096330 MtrunA17_Chr7g0274061 53.571 336 123 6 6 308 13 348 5.03e-122 353
Msa1096330 MtrunA17_Chr7g0274041 51.479 338 132 7 4 310 7 343 7.21e-114 332
Msa1096330 MtrunA17_Chr7g0221781 83.206 131 21 1 1 131 1 130 9.91e-75 226
Msa1096330 MtrunA17_Chr7g0274081 53.968 189 71 3 136 308 8 196 1.42e-71 219
Msa1096330 MtrunA17_Chr7g0221771 89.423 104 11 0 207 310 5 108 1.96e-65 201
Msa1096330 MtrunA17_Chr7g0222151 90.411 73 7 0 206 278 4 76 1.20e-45 149
Msa1096330 MtrunA17_Chr7g0222141 79.775 89 10 2 132 218 60 142 6.79e-42 142
Msa1096330 MtrunA17_Chr7g0221801 88.889 72 8 0 1 72 1 72 5.70e-41 137
Msa1096330 MtrunA17_Chr3g0095421 29.762 336 179 11 1 290 32 356 4.26e-30 117
Msa1096330 MtrunA17_Chr7g0274091 50.617 81 39 1 9 88 14 94 6.49e-20 83.6
Msa1096330 MtrunA17_Chr3g0127491 26.644 289 166 9 36 286 69 349 1.82e-19 87.8
Msa1096330 MtrunA17_Chr1g0169691 27.414 321 185 13 9 288 6 319 4.46e-17 80.5
Msa1096330 MtrunA17_Chr4g0018921 24.830 294 161 11 28 287 20 287 2.57e-13 69.3
Msa1096330 MtrunA17_Chr4g0049341 25.085 295 169 10 8 269 5 280 3.71e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1096330 AT1G60690.1 71.045 335 70 3 4 311 7 341 7.33e-174 485
Msa1096330 AT1G60730.1 71.642 335 68 3 4 311 7 341 8.00e-174 485
Msa1096330 AT1G60710.1 71.343 335 69 3 4 311 7 341 2.42e-173 484
Msa1096330 AT1G60680.1 70.238 336 72 4 4 311 7 342 1.01e-170 477
Msa1096330 AT1G60730.3 67.606 355 68 3 4 311 7 361 2.45e-170 477
Msa1096330 AT1G60750.1 70.462 325 73 4 4 311 8 326 1.02e-164 461
Msa1096330 AT1G10810.1 66.766 337 85 3 2 311 5 341 4.71e-161 452
Msa1096330 AT1G10810.3 65.672 268 65 3 71 311 22 289 1.04e-122 353
Msa1096330 AT1G60680.2 71.861 231 44 3 4 219 7 231 5.94e-115 332
Msa1096330 AT1G10810.2 62.846 253 70 2 2 240 5 247 3.70e-106 310
Msa1096330 AT1G60730.2 77.778 135 30 0 4 138 7 141 1.62e-72 222
Msa1096330 AT1G06690.1 28.485 330 181 11 6 290 48 367 5.68e-24 100
Msa1096330 AT1G04690.1 28.065 310 163 11 9 272 6 301 1.26e-21 93.6
Msa1096330 AT5G53580.1 29.433 282 147 10 46 286 80 350 5.34e-20 89.7
Msa1096330 AT1G04420.1 25.634 355 185 14 8 290 60 407 2.82e-17 82.0
Msa1096330 AT4G33670.1 25.552 317 200 11 1 290 1 308 3.27e-17 81.3

Find 103 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCCCTTCAATATATT+TGG 0.148190 7_3:+20655357 None:intergenic
TCCAATTGCACAGCTGTTAT+TGG 0.154954 7_3:+20654090 None:intergenic
TTGATTAAAGTTCTCCATTT+TGG 0.183974 7_3:+20652992 None:intergenic
ACCACTCTCTTCATGAAATA+AGG 0.206488 7_3:-20652844 Msa1096330:exon
TAAACGTTAGTTCTATTTAT+AGG 0.223507 7_3:+20652625 None:intergenic
AAGAAATAATTCCAACTTGC+AGG 0.276199 7_3:-20654038 Msa1096330:intron
CACACCAACGAACTCCTTCT+TGG 0.278970 7_3:-20655612 Msa1096330:CDS
CTTGATACCTCTGACATCTA+TGG 0.288925 7_3:-20655639 Msa1096330:CDS
GTTGAATTGGCTACAAAGTT+TGG 0.298080 7_3:-20655382 Msa1096330:CDS
TATCCTAATACTATGTAAAA+AGG 0.299919 7_3:+20655861 None:intergenic
GAAGGGAAATTTGAGATCTT+TGG 0.300695 7_3:-20655346 Msa1096330:CDS
AGACACACCAACGACGTATC+TGG 0.327505 7_3:+20652560 None:intergenic
TTGACTCGTCGCTCAATTAC+CGG 0.334229 7_3:-20652517 Msa1096330:three_prime_UTR
TTACATAGTATTAGGATAGA+AGG 0.335265 7_3:-20655856 Msa1096330:five_prime_UTR
TAAGAGTAAATAATTATGTT+AGG 0.358783 7_3:-20652691 Msa1096330:three_prime_UTR
AGTGAGAGAGAAGGTTGAAT+TGG 0.360017 7_3:-20655395 Msa1096330:CDS
AATCAAACCGTGATTTCAAT+TGG 0.361716 7_3:+20655223 None:intergenic
GATACCTGGAACCACCAAAA+TGG 0.362093 7_3:-20653006 Msa1096330:CDS
TCCAATAACAGCTGTGCAAT+TGG 0.363190 7_3:-20654091 Msa1096330:CDS
CAAAGTCAGAAAACTAACTA+TGG 0.364734 7_3:-20652760 Msa1096330:three_prime_UTR
TTTCTTAGGGAACTTGGTAT+TGG 0.374176 7_3:-20653662 Msa1096330:intron
GCTGTCAAGGGTGGTAGATA+TGG 0.379414 7_3:-20652903 Msa1096330:CDS
GTCGTTGGTGTGTCTCTGAT+TGG 0.392595 7_3:-20652552 Msa1096330:three_prime_UTR
CCACTCTCTTCATGAAATAA+GGG 0.410155 7_3:-20652843 Msa1096330:exon
ATTTGAGAGGGTTAATGAAC+TGG 0.426318 7_3:-20653102 Msa1096330:CDS
CTGTCAAGGGTGGTAGATAT+GGG 0.429429 7_3:-20652902 Msa1096330:CDS
TAAACTAACACAAGAAGAAA+TGG 0.436815 7_3:-20652949 Msa1096330:CDS
AGAGCAATCATGTCAGGTTC+AGG 0.437605 7_3:+20655696 None:intergenic
CTTGGTTGCATGAGCATGTC+AGG 0.442441 7_3:-20655738 Msa1096330:CDS
GCAGTAGGAAGAATGAAGTT+AGG 0.442452 7_3:-20655792 Msa1096330:CDS
AAAAGTGACACCAGATTGTA+TGG 0.442868 7_3:+20655662 None:intergenic
ATATAGTCTGATTCTGCTGC+AGG 0.450000 7_3:+20653123 None:intergenic
TCGTCGCTCAATTACCGGTT+GGG 0.450519 7_3:-20652512 Msa1096330:three_prime_UTR
CAAGCTTCTCTCACGTAAGC+TGG 0.451335 7_3:+20655319 None:intergenic
GAAGTTGGTAGCATATTGAT+TGG 0.462888 7_3:+20652582 None:intergenic
ATTGTTGCATATAGTCCTCT+TGG 0.471189 7_3:-20653638 Msa1096330:CDS
AATTGTGGACAGGCTTTAAG+AGG 0.474129 7_3:-20655421 Msa1096330:intron
TTTGGAGCCAAATATATTGA+AGG 0.475808 7_3:-20655364 Msa1096330:CDS
CTCGTCGCTCAATTACCGGT+TGG 0.477440 7_3:-20652513 Msa1096330:three_prime_UTR
TAATGAACTGGCTACAAAGA+AGG 0.479658 7_3:-20653090 Msa1096330:CDS
TCGGGCACACGTCGTTTCCT+TGG 0.481328 7_3:+20653027 None:intergenic
ATTCACCATGCCATACAATC+TGG 0.483546 7_3:-20655672 Msa1096330:CDS
AAGAGGAGGAGTGAGAGAGA+AGG 0.485439 7_3:-20655404 Msa1096330:CDS
ATAAATAAACCTGGTGGTGG+CGG 0.497611 7_3:-20655912 Msa1096330:five_prime_UTR
CGTCTCAGCTTGCATTGGCA+TGG 0.497948 7_3:-20653058 Msa1096330:CDS
ACTATGGAGATTTGCATCAA+TGG 0.497987 7_3:-20652744 Msa1096330:three_prime_UTR
ATGAGCATGTCAGGTGTCTA+TGG 0.498898 7_3:-20655729 Msa1096330:CDS
TGCAATTGGAGTGGTCACTA+TGG 0.500317 7_3:-20654077 Msa1096330:CDS
ATCATGTCAGGTTCAGGCTT+AGG 0.505151 7_3:+20655702 None:intergenic
GAAGTGTCCTTGCAAGGACT+TGG 0.508887 7_3:-20655756 Msa1096330:CDS
AACGACGTGTGCCCGATACC+TGG 0.512377 7_3:-20653020 Msa1096330:CDS
GTAGATATGTGGAAAATGCT+TGG 0.524452 7_3:-20652818 Msa1096330:three_prime_UTR
GTCTTTAGCAGACGCTGTCA+AGG 0.529986 7_3:-20652916 Msa1096330:CDS
TCGTCTTCCAATTGAAATCA+CGG 0.530494 7_3:-20655230 Msa1096330:intron
GAAGTTAGGATCACAAGGAA+TGG 0.532873 7_3:-20655778 Msa1096330:CDS
TCTTCTGATTGTTGAAGCTG+AGG 0.532875 7_3:+20654128 None:intergenic
AAATAAGGGTGGTAGATATG+TGG 0.535749 7_3:-20652829 Msa1096330:three_prime_UTR
ACTAAATCAAACCTGCAAGT+TGG 0.537019 7_3:+20654027 None:intergenic
AGAAGAACAATGACAGCAGT+AGG 0.537429 7_3:-20655807 Msa1096330:exon
TACTCCGTCTCAGCTTGCAT+TGG 0.539631 7_3:-20653063 Msa1096330:CDS
TGAATATCTCCGCCACCACC+AGG 0.540225 7_3:+20655903 None:intergenic
ATATGTGGAAAATGCTTGGA+AGG 0.540917 7_3:-20652814 Msa1096330:three_prime_UTR
TAATTGAGCGACGAGTCAAC+GGG 0.542980 7_3:+20652521 None:intergenic
AAGTAAGAACCTTTCCAAGA+AGG 0.544038 7_3:+20655598 None:intergenic
GAGAACTTTAATCAAAACAT+TGG 0.545978 7_3:-20652984 Msa1096330:CDS
GAAGGAGTTCGTTGGTGTGA+GGG 0.546165 7_3:+20655616 None:intergenic
TCGTTGGTGTGTCTCTGATT+GGG 0.546299 7_3:-20652551 Msa1096330:three_prime_UTR
AATGAACTGGCTACAAAGAA+GGG 0.546567 7_3:-20653089 Msa1096330:CDS
CTTTCCAAGAAGGAGTTCGT+TGG 0.553887 7_3:+20655608 None:intergenic
CATGCCAATGCAAGCTGAGA+CGG 0.554180 7_3:+20653059 None:intergenic
CAACGAACTCCTTCTTGGAA+AGG 0.555048 7_3:-20655607 Msa1096330:intron
GTAATTGAGCGACGAGTCAA+CGG 0.556257 7_3:+20652520 None:intergenic
ATGTCAGGTTCAGGCTTAGG+AGG 0.557823 7_3:+20655705 None:intergenic
TGAATGAGAGCAATCATGTC+AGG 0.558885 7_3:+20655690 None:intergenic
TGGTGTGTCTCTGATTGGGC+AGG 0.560605 7_3:-20652547 Msa1096330:three_prime_UTR
CATTCAGAAATTTCTAAGTG+TGG 0.561408 7_3:-20652721 Msa1096330:three_prime_UTR
TTGGAGCCAAATATATTGAA+GGG 0.564347 7_3:-20655363 Msa1096330:CDS
GCATATAGTCCTCTTGGACG+AGG 0.577164 7_3:-20653632 Msa1096330:CDS
CCAACGACGTATCTGGAAGT+TGG 0.580472 7_3:+20652567 None:intergenic
AGAATCAGACTATATTTGAG+AGG 0.585843 7_3:-20653115 Msa1096330:CDS
CATGCAACCAAGTCCTTGCA+AGG 0.590784 7_3:+20655749 None:intergenic
GGAATGGAAGTGTCCTTGCA+AGG 0.592055 7_3:-20655762 Msa1096330:CDS
CTTATAAATAAACCTGGTGG+TGG 0.594211 7_3:-20655915 Msa1096330:five_prime_UTR
AGAAGGAGTTCGTTGGTGTG+AGG 0.603074 7_3:+20655615 None:intergenic
TCTTTAGCAGACGCTGTCAA+GGG 0.604096 7_3:-20652915 Msa1096330:CDS
GAATCAGACTATATTTGAGA+GGG 0.604511 7_3:-20653114 Msa1096330:CDS
ATTGCTCTTATAAATAAACC+TGG 0.610224 7_3:-20655921 Msa1096330:five_prime_UTR
GAAAAGAATCCTCGTCCAAG+AGG 0.611487 7_3:+20653623 None:intergenic
ACTATGGTCAAGAGATGTTG+AGG 0.614034 7_3:-20654061 Msa1096330:CDS
TGTGGACAGGCTTTAAGAGG+AGG 0.614236 7_3:-20655418 Msa1096330:intron
ATGGGGATGAAATAAGTACA+TGG 0.614624 7_3:-20652884 Msa1096330:CDS
CCCTTATTTCATGAAGAGAG+TGG 0.618669 7_3:+20652843 None:intergenic
TTATTTCATGAAGAGAGTGG+TGG 0.633297 7_3:+20652846 None:intergenic
CTCTCTTCATGAAATAAGGG+TGG 0.633642 7_3:-20652840 Msa1096330:exon
TTAGCAGACGCTGTCAAGGG+TGG 0.635070 7_3:-20652912 Msa1096330:CDS
AGAATGAAGTTAGGATCACA+AGG 0.639560 7_3:-20655783 Msa1096330:CDS
TAACAGCTGTGCAATTGGAG+TGG 0.661184 7_3:-20654086 Msa1096330:CDS
TGTCAAGGGTGGTAGATATG+GGG 0.665647 7_3:-20652901 Msa1096330:CDS
GTGAGGGCCATAGATGTCAG+AGG 0.676572 7_3:+20655632 None:intergenic
TTGGCATGGCTTCATCACCA+AGG 0.680145 7_3:-20653044 Msa1096330:CDS
CCAACTTCCAGATACGTCGT+TGG 0.689547 7_3:-20652567 Msa1096330:three_prime_UTR
GCTCTTATAAATAAACCTGG+TGG 0.694693 7_3:-20655918 Msa1096330:five_prime_UTR
TGACACCAGATTGTATGGCA+TGG 0.709987 7_3:+20655667 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGATTTTTTTTTTCTTA+GGG - chr7_3:20654773-20654792 Msa1096330:intron 10.0%
!!! TAATTAAAATAGAACATTTT+GGG + chr7_3:20655188-20655207 None:intergenic 10.0%
!! TATAAAAATGTGTTTCAATA+TGG + chr7_3:20653333-20653352 None:intergenic 15.0%
!! TATTACCTAAAATTAATGTT+TGG - chr7_3:20653355-20653374 Msa1096330:intron 15.0%
!!! TTTATCCAAACATTAATTTT+AGG + chr7_3:20653363-20653382 None:intergenic 15.0%
!!! TGGATAAATTATTTTCAAAA+AGG - chr7_3:20653375-20653394 Msa1096330:intron 15.0%
!! CAATTAAAATTTGAATCTAA+AGG - chr7_3:20653773-20653792 Msa1096330:intron 15.0%
!! TTTAATATAATATTGGCATT+GGG + chr7_3:20654017-20654036 None:intergenic 15.0%
!! ATTTAATATAATATTGGCAT+TGG + chr7_3:20654018-20654037 None:intergenic 15.0%
!! ATGATTGTAATTTAAGAAAA+TGG - chr7_3:20654530-20654549 Msa1096330:intron 15.0%
!!! GTTATGATTTTTTTTTTCTT+AGG - chr7_3:20654772-20654791 Msa1096330:intron 15.0%
!!! CTAATTAAAATAGAACATTT+TGG + chr7_3:20655189-20655208 None:intergenic 15.0%
!! AGATATTTAATAATTGATGT+AGG + chr7_3:20655213-20655232 None:intergenic 15.0%
!! ACAAATAGAAGATTATATTT+AGG + chr7_3:20655267-20655286 None:intergenic 15.0%
!! TAAGAGTAAATAATTATGTT+AGG - chr7_3:20655757-20655776 Msa1096330:CDS 15.0%
!! TATCCTAATACTATGTAAAA+AGG + chr7_3:20652590-20652609 None:intergenic 20.0%
!! AAATTTCCCTTCAATATATT+TGG + chr7_3:20653094-20653113 None:intergenic 20.0%
!!! AAAATTCTTTGATTCCATTT+TGG - chr7_3:20653254-20653273 Msa1096330:intron 20.0%
!!! CAAATGATTTGATTTTTGTT+TGG - chr7_3:20654058-20654077 Msa1096330:CDS 20.0%
!! TTAAAATTGCAATGATATGA+AGG - chr7_3:20654493-20654512 Msa1096330:intron 20.0%
!! AAAAAAAATCATAACTCATG+AGG + chr7_3:20654767-20654786 None:intergenic 20.0%
!!! TAAACGTTAGTTCTATTTAT+AGG + chr7_3:20655826-20655845 None:intergenic 20.0%
! ATTGCTCTTATAAATAAACC+TGG - chr7_3:20652527-20652546 Msa1096330:three_prime_UTR 25.0%
!! TTCACAACTTGAATTTTGTT+TGG - chr7_3:20652564-20652583 Msa1096330:three_prime_UTR 25.0%
! TTACATAGTATTAGGATAGA+AGG - chr7_3:20652592-20652611 Msa1096330:three_prime_UTR 25.0%
! AACTTTCTCTCTTAATCTAA+CGG + chr7_3:20653435-20653454 None:intergenic 25.0%
! TACTAGACAATCATAAGAAA+AGG - chr7_3:20653861-20653880 Msa1096330:intron 25.0%
! TTAATAAAGGTGATATCCAT+TGG + chr7_3:20653981-20654000 None:intergenic 25.0%
! AAGAGACATCACTTTAATAA+AGG + chr7_3:20653994-20654013 None:intergenic 25.0%
! CTTAAAAAACTAGTTGAAGA+GGG - chr7_3:20654268-20654287 Msa1096330:intron 25.0%
! TTGAATGTACTTACAAGTAT+TGG - chr7_3:20654559-20654578 Msa1096330:intron 25.0%
!!! TTTTTTTTTCTTAGGGAACT+TGG - chr7_3:20654780-20654799 Msa1096330:intron 25.0%
! TATTAATGATGTCTATGTGT+CGG + chr7_3:20655021-20655040 None:intergenic 25.0%
! TGTTATATAGATTCTAACCT+TGG - chr7_3:20655159-20655178 Msa1096330:intron 25.0%
!! TTGATTAAAGTTCTCCATTT+TGG + chr7_3:20655459-20655478 None:intergenic 25.0%
! GAGAACTTTAATCAAAACAT+TGG - chr7_3:20655464-20655483 Msa1096330:intron 25.0%
! TAAACTAACACAAGAAGAAA+TGG - chr7_3:20655499-20655518 Msa1096330:intron 25.0%
! TGGCCTTTTTACATAGTATT+AGG - chr7_3:20652584-20652603 Msa1096330:three_prime_UTR 30.0%
AATGTGTATGTGTGAAATTG+TGG - chr7_3:20653012-20653031 Msa1096330:CDS 30.0%
! TTTGGAGCCAAATATATTGA+AGG - chr7_3:20653084-20653103 Msa1096330:CDS 30.0%
TTGGAGCCAAATATATTGAA+GGG - chr7_3:20653085-20653104 Msa1096330:CDS 30.0%
AATCAAACCGTGATTTCAAT+TGG + chr7_3:20653228-20653247 None:intergenic 30.0%
CTCAATTTAAGCTACCAAAA+TGG + chr7_3:20653271-20653290 None:intergenic 30.0%
ATAATTGAACCATAGTCACA+TGG - chr7_3:20653595-20653614 Msa1096330:intron 30.0%
GGTACGCTCAAATATAATAT+TGG - chr7_3:20653725-20653744 Msa1096330:intron 30.0%
!! TAATATTGGTGCACTAGTTT+TGG - chr7_3:20653739-20653758 Msa1096330:intron 30.0%
AATTTGAATCTAAAGGCTGA+TGG - chr7_3:20653780-20653799 Msa1096330:intron 30.0%
! TTTAGAAACATGATGGAAAC+AGG - chr7_3:20653810-20653829 Msa1096330:intron 30.0%
TTTACAAATAAGTCCCACAT+CGG - chr7_3:20653833-20653852 Msa1096330:intron 30.0%
ATAATACTAGTACAAGACGT+AGG + chr7_3:20653928-20653947 None:intergenic 30.0%
!! TTGATTTTTGTTTGGTACGT+TGG - chr7_3:20654066-20654085 Msa1096330:CDS 30.0%
!! TGATTTTTGTTTGGTACGTT+GGG - chr7_3:20654067-20654086 Msa1096330:CDS 30.0%
CTCTACAGTTATCGAATTAT+AGG - chr7_3:20654092-20654111 Msa1096330:CDS 30.0%
TCGAATTATAGGAGAATTAC+TGG - chr7_3:20654103-20654122 Msa1096330:CDS 30.0%
! TCCCTTTTACTATGTAAGAT+TGG - chr7_3:20654241-20654260 Msa1096330:intron 30.0%
CCCAATCTTACATAGTAAAA+GGG + chr7_3:20654245-20654264 None:intergenic 30.0%
! CCCTTTTACTATGTAAGATT+GGG - chr7_3:20654242-20654261 Msa1096330:intron 30.0%
! CCTTTTACTATGTAAGATTG+GGG - chr7_3:20654243-20654262 Msa1096330:intron 30.0%
GCTTAAAAAACTAGTTGAAG+AGG - chr7_3:20654267-20654286 Msa1096330:intron 30.0%
GAGGGAAAAATAAAGTACAT+TGG - chr7_3:20654286-20654305 Msa1096330:intron 30.0%
AAGAAATAATTCCAACTTGC+AGG - chr7_3:20654410-20654429 Msa1096330:intron 30.0%
GATTGTTGAGAACTTAACAA+AGG - chr7_3:20654860-20654879 Msa1096330:intron 30.0%
AACTGACAAGTATGAAATAC+TGG - chr7_3:20654963-20654982 Msa1096330:intron 30.0%
AGAATCAGACTATATTTGAG+AGG - chr7_3:20655333-20655352 Msa1096330:CDS 30.0%
GAATCAGACTATATTTGAGA+GGG - chr7_3:20655334-20655353 Msa1096330:CDS 30.0%
! ATTAAAGTTCTCCATTTTGG+TGG + chr7_3:20655456-20655475 None:intergenic 30.0%
CAAAGTCAGAAAACTAACTA+TGG - chr7_3:20655688-20655707 Msa1096330:CDS 30.0%
CATTCAGAAATTTCTAAGTG+TGG - chr7_3:20655727-20655746 Msa1096330:CDS 30.0%
GCTCTTATAAATAAACCTGG+TGG - chr7_3:20652530-20652549 Msa1096330:three_prime_UTR 35.0%
CTTATAAATAAACCTGGTGG+TGG - chr7_3:20652533-20652552 Msa1096330:three_prime_UTR 35.0%
AGAATGAAGTTAGGATCACA+AGG - chr7_3:20652665-20652684 Msa1096330:three_prime_UTR 35.0%
AAAAGTGACACCAGATTGTA+TGG + chr7_3:20652789-20652808 None:intergenic 35.0%
AAGTAAGAACCTTTCCAAGA+AGG + chr7_3:20652853-20652872 None:intergenic 35.0%
GTTGAATTGGCTACAAAGTT+TGG - chr7_3:20653066-20653085 Msa1096330:CDS 35.0%
! GAAGGGAAATTTGAGATCTT+TGG - chr7_3:20653102-20653121 Msa1096330:CDS 35.0%
TCGTCTTCCAATTGAAATCA+CGG - chr7_3:20653218-20653237 Msa1096330:intron 35.0%
CTGAAGCAAACATATGCTTA+TGG + chr7_3:20653296-20653315 None:intergenic 35.0%
TTCGTCAGTCTCAAAGAATT+CGG - chr7_3:20653661-20653680 Msa1096330:intron 35.0%
! AAGCTGGTTTAGAAACATGA+TGG - chr7_3:20653803-20653822 Msa1096330:intron 35.0%
GTCTAGTAAATAACCGATGT+GGG + chr7_3:20653849-20653868 None:intergenic 35.0%
TGTCTAGTAAATAACCGATG+TGG + chr7_3:20653850-20653869 None:intergenic 35.0%
CCCCAATCTTACATAGTAAA+AGG + chr7_3:20654246-20654265 None:intergenic 35.0%
ACTAAATCAAACCTGCAAGT+TGG + chr7_3:20654424-20654443 None:intergenic 35.0%
TTATCTAGCATTTCTCTCTG+TGG - chr7_3:20654463-20654482 Msa1096330:intron 35.0%
AACGATTAAAGACGTGTGTT+TGG + chr7_3:20654719-20654738 None:intergenic 35.0%
AAACACACGTCTTTAATCGT+TGG - chr7_3:20654718-20654737 Msa1096330:intron 35.0%
TTTCTTAGGGAACTTGGTAT+TGG - chr7_3:20654786-20654805 Msa1096330:intron 35.0%
ATTGTTGCATATAGTCCTCT+TGG - chr7_3:20654810-20654829 Msa1096330:intron 35.0%
TTTCATACTTGTCAGTTGAG+TGG + chr7_3:20654960-20654979 None:intergenic 35.0%
ATGAAATACTGGCACAAACA+TGG - chr7_3:20654974-20654993 Msa1096330:intron 35.0%
TCACATGTTCAGTATTGTGT+TGG - chr7_3:20655081-20655100 Msa1096330:intron 35.0%
! ACACGAAACCTTCAAGTTTA+AGG + chr7_3:20655142-20655161 None:intergenic 35.0%
!!! AGGTTTTCAGGTTGAAATCT+AGG + chr7_3:20655308-20655327 None:intergenic 35.0%
ATTTGAGAGGGTTAATGAAC+TGG - chr7_3:20655346-20655365 Msa1096330:CDS 35.0%
TAATGAACTGGCTACAAAGA+AGG - chr7_3:20655358-20655377 Msa1096330:CDS 35.0%
AATGAACTGGCTACAAAGAA+GGG - chr7_3:20655359-20655378 Msa1096330:CDS 35.0%
ATGGGGATGAAATAAGTACA+TGG - chr7_3:20655564-20655583 Msa1096330:intron 35.0%
TTATTTCATGAAGAGAGTGG+TGG + chr7_3:20655605-20655624 None:intergenic 35.0%
ACCACTCTCTTCATGAAATA+AGG - chr7_3:20655604-20655623 Msa1096330:intron 35.0%
CCACTCTCTTCATGAAATAA+GGG - chr7_3:20655605-20655624 Msa1096330:intron 35.0%
AAATAAGGGTGGTAGATATG+TGG - chr7_3:20655619-20655638 Msa1096330:CDS 35.0%
GTAGATATGTGGAAAATGCT+TGG - chr7_3:20655630-20655649 Msa1096330:CDS 35.0%
ATATGTGGAAAATGCTTGGA+AGG - chr7_3:20655634-20655653 Msa1096330:CDS 35.0%
ACTTTGCTGAAGGCAAAAAT+AGG + chr7_3:20655674-20655693 None:intergenic 35.0%
! TAGTTTTCTGACTTTGCTGA+AGG + chr7_3:20655684-20655703 None:intergenic 35.0%
ACTATGGAGATTTGCATCAA+TGG - chr7_3:20655704-20655723 Msa1096330:CDS 35.0%
!! GAAGTTGGTAGCATATTGAT+TGG + chr7_3:20655869-20655888 None:intergenic 35.0%
!! ATAAATAAACCTGGTGGTGG+CGG - chr7_3:20652536-20652555 Msa1096330:three_prime_UTR 40.0%
AGAAGAACAATGACAGCAGT+AGG - chr7_3:20652641-20652660 Msa1096330:three_prime_UTR 40.0%
GCAGTAGGAAGAATGAAGTT+AGG - chr7_3:20652656-20652675 Msa1096330:three_prime_UTR 40.0%
GAAGTTAGGATCACAAGGAA+TGG - chr7_3:20652670-20652689 Msa1096330:three_prime_UTR 40.0%
TGAATGAGAGCAATCATGTC+AGG + chr7_3:20652761-20652780 None:intergenic 40.0%
ATTCACCATGCCATACAATC+TGG - chr7_3:20652776-20652795 Msa1096330:three_prime_UTR 40.0%
CTTGATACCTCTGACATCTA+TGG - chr7_3:20652809-20652828 Msa1096330:three_prime_UTR 40.0%
GTATGTGTGAAATTGTGGAC+AGG - chr7_3:20653017-20653036 Msa1096330:CDS 40.0%
!! AATTGTGGACAGGCTTTAAG+AGG - chr7_3:20653027-20653046 Msa1096330:CDS 40.0%
AGTGAGAGAGAAGGTTGAAT+TGG - chr7_3:20653053-20653072 Msa1096330:CDS 40.0%
CTTTCTCAATCTACGATCAG+CGG - chr7_3:20653459-20653478 Msa1096330:intron 40.0%
AATTGCGAACCATGTGACTA+TGG + chr7_3:20653607-20653626 None:intergenic 40.0%
TCAAAGAATTCGGTACGTGA+GGG - chr7_3:20653671-20653690 Msa1096330:intron 40.0%
GCATATGCTTTAGCATATGC+TGG - chr7_3:20653950-20653969 Msa1096330:intron 40.0%
TATGCTTTAGCATATGCTGG+TGG - chr7_3:20653953-20653972 Msa1096330:intron 40.0%
ATGCTTTAGCATATGCTGGT+GGG - chr7_3:20653954-20653973 Msa1096330:intron 40.0%
TCTTCTGATTGTTGAAGCTG+AGG + chr7_3:20654323-20654342 None:intergenic 40.0%
TCCAATAACAGCTGTGCAAT+TGG - chr7_3:20654357-20654376 Msa1096330:intron 40.0%
TCCAATTGCACAGCTGTTAT+TGG + chr7_3:20654361-20654380 None:intergenic 40.0%
ACTATGGTCAAGAGATGTTG+AGG - chr7_3:20654387-20654406 Msa1096330:intron 40.0%
CGAACACACCTTAAACTTGA+AGG - chr7_3:20655131-20655150 Msa1096330:intron 40.0%
!!! TAGAACATTTTGGGATGCCA+AGG + chr7_3:20655179-20655198 None:intergenic 40.0%
ATATAGTCTGATTCTGCTGC+AGG + chr7_3:20655328-20655347 None:intergenic 40.0%
!! ATTTTGGTGGTTCCAGGTAT+CGG + chr7_3:20655443-20655462 None:intergenic 40.0%
! CCCTTATTTCATGAAGAGAG+TGG + chr7_3:20655608-20655627 None:intergenic 40.0%
CTCTCTTCATGAAATAAGGG+TGG - chr7_3:20655608-20655627 Msa1096330:CDS 40.0%
ATGAGCATGTCAGGTGTCTA+TGG - chr7_3:20652719-20652738 Msa1096330:three_prime_UTR 45.0%
ATCATGTCAGGTTCAGGCTT+AGG + chr7_3:20652749-20652768 None:intergenic 45.0%
AGAGCAATCATGTCAGGTTC+AGG + chr7_3:20652755-20652774 None:intergenic 45.0%
TGACACCAGATTGTATGGCA+TGG + chr7_3:20652784-20652803 None:intergenic 45.0%
CTTTCCAAGAAGGAGTTCGT+TGG + chr7_3:20652843-20652862 None:intergenic 45.0%
CAACGAACTCCTTCTTGGAA+AGG - chr7_3:20652841-20652860 Msa1096330:exon 45.0%
AAGTCACTTAGCTCGACGTT+AGG + chr7_3:20652976-20652995 None:intergenic 45.0%
ACATGGTTCGCAATTCGCTT+CGG - chr7_3:20653612-20653631 Msa1096330:CDS 45.0%
CTCAAAGAATTCGGTACGTG+AGG - chr7_3:20653670-20653689 Msa1096330:intron 45.0%
CAAAGAATTCGGTACGTGAG+GGG - chr7_3:20653672-20653691 Msa1096330:intron 45.0%
AAAGAATTCGGTACGTGAGG+GGG - chr7_3:20653673-20653692 Msa1096330:intron 45.0%
TATACAGAGTCGGTACGCTA+TGG - chr7_3:20653696-20653715 Msa1096330:intron 45.0%
ATACAGAGTCGGTACGCTAT+GGG - chr7_3:20653697-20653716 Msa1096330:intron 45.0%
ATCTAAAGGCTGATGGAAGC+TGG - chr7_3:20653787-20653806 Msa1096330:intron 45.0%
ACTAGTACAAGACGTAGGCT+TGG + chr7_3:20653923-20653942 None:intergenic 45.0%
TGCTTTAGCATATGCTGGTG+GGG - chr7_3:20653955-20653974 Msa1096330:intron 45.0%
TAGGAGAATTACTGGCGAAC+CGG - chr7_3:20654111-20654130 Msa1096330:CDS 45.0%
TAACAGCTGTGCAATTGGAG+TGG - chr7_3:20654362-20654381 Msa1096330:intron 45.0%
TGCAATTGGAGTGGTCACTA+TGG - chr7_3:20654371-20654390 Msa1096330:intron 45.0%
!! CTTTAATCGTTGGTGCTGCA+TGG - chr7_3:20654728-20654747 Msa1096330:intron 45.0%
GAAAAGAATCCTCGTCCAAG+AGG + chr7_3:20654828-20654847 None:intergenic 45.0%
! GGACGAGGATTCTTTTCATC+AGG - chr7_3:20654831-20654850 Msa1096330:intron 45.0%
AACAAAGGATGACTAACGCC+AGG - chr7_3:20654875-20654894 Msa1096330:intron 45.0%
! AGCATGCTTTTCGACAAACC+TGG + chr7_3:20654896-20654915 None:intergenic 45.0%
! TGATTCTGCTGCAGGTTTTC+AGG + chr7_3:20655320-20655339 None:intergenic 45.0%
!! TTTTGGTGGTTCCAGGTATC+GGG + chr7_3:20655442-20655461 None:intergenic 45.0%
GATACCTGGAACCACCAAAA+TGG - chr7_3:20655442-20655461 Msa1096330:intron 45.0%
!! TTCTCCATTTTGGTGGTTCC+AGG + chr7_3:20655449-20655468 None:intergenic 45.0%
TCTTTAGCAGACGCTGTCAA+GGG - chr7_3:20655533-20655552 Msa1096330:intron 45.0%
CTGTCAAGGGTGGTAGATAT+GGG - chr7_3:20655546-20655565 Msa1096330:intron 45.0%
TGTCAAGGGTGGTAGATATG+GGG - chr7_3:20655547-20655566 Msa1096330:intron 45.0%
TCGTTGGTGTGTCTCTGATT+GGG - chr7_3:20655897-20655916 Msa1096330:five_prime_UTR 45.0%
TAATTGAGCGACGAGTCAAC+GGG + chr7_3:20655930-20655949 None:intergenic 45.0%
GTAATTGAGCGACGAGTCAA+CGG + chr7_3:20655931-20655950 None:intergenic 45.0%
! TTGACTCGTCGCTCAATTAC+CGG - chr7_3:20655931-20655950 Msa1096330:five_prime_UTR 45.0%
!! TACCGGTTGGGTTGTGTTTT+TGG - chr7_3:20655948-20655967 Msa1096330:five_prime_UTR 45.0%
AGCCAAAAACACAACCCAAC+CGG + chr7_3:20655953-20655972 None:intergenic 45.0%
!! AAACTAATTTAATATAATAT+TGG + chr7_3:20654024-20654043 None:intergenic 5.0%
!! GGAATGGAAGTGTCCTTGCA+AGG - chr7_3:20652686-20652705 Msa1096330:three_prime_UTR 50.0%
! GAAGTGTCCTTGCAAGGACT+TGG - chr7_3:20652692-20652711 Msa1096330:three_prime_UTR 50.0%
CATGCAACCAAGTCCTTGCA+AGG + chr7_3:20652702-20652721 None:intergenic 50.0%
CTTGGTTGCATGAGCATGTC+AGG - chr7_3:20652710-20652729 Msa1096330:three_prime_UTR 50.0%
ATGTCAGGTTCAGGCTTAGG+AGG + chr7_3:20652746-20652765 None:intergenic 50.0%
GAAGGAGTTCGTTGGTGTGA+GGG + chr7_3:20652835-20652854 None:intergenic 50.0%
AGAAGGAGTTCGTTGGTGTG+AGG + chr7_3:20652836-20652855 None:intergenic 50.0%
CACACCAACGAACTCCTTCT+TGG - chr7_3:20652836-20652855 Msa1096330:exon 50.0%
!! TGTGGACAGGCTTTAAGAGG+AGG - chr7_3:20653030-20653049 Msa1096330:CDS 50.0%
AAGAGGAGGAGTGAGAGAGA+AGG - chr7_3:20653044-20653063 Msa1096330:CDS 50.0%
CAAGCTTCTCTCACGTAAGC+TGG + chr7_3:20653132-20653151 None:intergenic 50.0%
TCAATCTACGATCAGCGGCA+TGG - chr7_3:20653464-20653483 Msa1096330:intron 50.0%
ATTCGCTTCGGTACGTGGTA+CGG - chr7_3:20653624-20653643 Msa1096330:CDS 50.0%
AGGAGAATTACTGGCGAACC+GGG - chr7_3:20654112-20654131 Msa1096330:CDS 50.0%
GCATATAGTCCTCTTGGACG+AGG - chr7_3:20654816-20654835 Msa1096330:intron 50.0%
GTCGGTGACACTATCATGTC+AGG + chr7_3:20655003-20655022 None:intergenic 50.0%
TACTCCGTCTCAGCTTGCAT+TGG - chr7_3:20655385-20655404 Msa1096330:CDS 50.0%
CATGCCAATGCAAGCTGAGA+CGG + chr7_3:20655392-20655411 None:intergenic 50.0%
TTGGCATGGCTTCATCACCA+AGG - chr7_3:20655404-20655423 Msa1096330:CDS 50.0%
GTCTTTAGCAGACGCTGTCA+AGG - chr7_3:20655532-20655551 Msa1096330:intron 50.0%
GCTGTCAAGGGTGGTAGATA+TGG - chr7_3:20655545-20655564 Msa1096330:intron 50.0%
CCAACGACGTATCTGGAAGT+TGG + chr7_3:20655884-20655903 None:intergenic 50.0%
CCAACTTCCAGATACGTCGT+TGG - chr7_3:20655881-20655900 Msa1096330:five_prime_UTR 50.0%
AGACACACCAACGACGTATC+TGG + chr7_3:20655891-20655910 None:intergenic 50.0%
GTCGTTGGTGTGTCTCTGAT+TGG - chr7_3:20655896-20655915 Msa1096330:five_prime_UTR 50.0%
! TCGTCGCTCAATTACCGGTT+GGG - chr7_3:20655936-20655955 Msa1096330:five_prime_UTR 50.0%
TGAATATCTCCGCCACCACC+AGG + chr7_3:20652548-20652567 None:intergenic 55.0%
GTGAGGGCCATAGATGTCAG+AGG + chr7_3:20652819-20652838 None:intergenic 55.0%
TCGCAATTCGCTTCGGTACG+TGG - chr7_3:20653619-20653638 Msa1096330:CDS 55.0%
TTCGCTTCGGTACGTGGTAC+GGG - chr7_3:20653625-20653644 Msa1096330:CDS 55.0%
TTCGGTACGTGGTACGGGTT+CGG - chr7_3:20653630-20653649 Msa1096330:CDS 55.0%
! CGTGAGGGGGTATACAGAGT+CGG - chr7_3:20653686-20653705 Msa1096330:intron 55.0%
GTACAAGACGTAGGCTTGGC+TGG + chr7_3:20653919-20653938 None:intergenic 55.0%
TTCGCGGTACTGTTACGACC+CGG + chr7_3:20654133-20654152 None:intergenic 55.0%
GTCGTAACAGTACCGCGAAC+CGG - chr7_3:20654134-20654153 Msa1096330:CDS 55.0%
TTACCTGAGAACCGCGAACC+CGG + chr7_3:20654169-20654188 None:intergenic 55.0%
! CGTCTCAGCTTGCATTGGCA+TGG - chr7_3:20655390-20655409 Msa1096330:CDS 55.0%
TTAGCAGACGCTGTCAAGGG+TGG - chr7_3:20655536-20655555 Msa1096330:intron 55.0%
TGGTGTGTCTCTGATTGGGC+AGG - chr7_3:20655901-20655920 Msa1096330:five_prime_UTR 55.0%
CTCGTCGCTCAATTACCGGT+TGG - chr7_3:20655935-20655954 Msa1096330:five_prime_UTR 55.0%
TCGGTACGTGGTACGGGTTC+GGG - chr7_3:20653631-20653650 Msa1096330:CDS 60.0%
GTCGGTACGCTATGGGTTCG+TGG - chr7_3:20653704-20653723 Msa1096330:intron 60.0%
GCATATGCTGGTGGGGCCAA+TGG - chr7_3:20653962-20653981 Msa1096330:intron 60.0%
TGAGAACCGCGAACCCGGTT+CGG + chr7_3:20654164-20654183 None:intergenic 60.0%
TCGGGCACACGTCGTTTCCT+TGG + chr7_3:20655424-20655443 None:intergenic 60.0%
AACGACGTGTGCCCGATACC+TGG - chr7_3:20655428-20655447 Msa1096330:intron 60.0%
GTGGTACGGGTTCGGGTACG+CGG - chr7_3:20653638-20653657 Msa1096330:CDS 65.0%
GAACCGGGTTCGCGGTTCTC+AGG - chr7_3:20654163-20654182 Msa1096330:intron 65.0%
!! CGGTTCGGCGAACCGGTTCG+CGG + chr7_3:20654149-20654168 None:intergenic 70.0%
!! CGCGAACCGGTTCGCCGAAC+CGG - chr7_3:20654147-20654166 Msa1096330:CDS 70.0%
!! GCGAACCGGTTCGCCGAACC+GGG - chr7_3:20654148-20654167 Msa1096330:CDS 70.0%
!! GCGAACCCGGTTCGGCGAAC+CGG + chr7_3:20654156-20654175 None:intergenic 70.0%
GGTTCGCCGAACCGGGTTCG+CGG - chr7_3:20654155-20654174 Msa1096330:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 20652490 20655980 20652490 ID=Msa1096330;Name=Msa1096330
chr7_3 mRNA 20652490 20655980 20652490 ID=Msa1096330-mRNA-1;Parent=Msa1096330;Name=Msa1096330-mRNA-1;_AED=0.29;_eAED=0.29;_QI=160|0.5|0.6|1|0.75|0.8|5|361|311
chr7_3 exon 20652490 20653171 20652490 ID=Msa1096330-mRNA-1:exon:4472;Parent=Msa1096330-mRNA-1
chr7_3 exon 20653607 20653676 20653607 ID=Msa1096330-mRNA-1:exon:4471;Parent=Msa1096330-mRNA-1
chr7_3 exon 20654039 20654169 20654039 ID=Msa1096330-mRNA-1:exon:4470;Parent=Msa1096330-mRNA-1
chr7_3 exon 20655231 20655431 20655231 ID=Msa1096330-mRNA-1:exon:4469;Parent=Msa1096330-mRNA-1
chr7_3 exon 20655608 20655980 20655608 ID=Msa1096330-mRNA-1:exon:4468;Parent=Msa1096330-mRNA-1
chr7_3 five_prime_UTR 20655821 20655980 20655821 ID=Msa1096330-mRNA-1:five_prime_utr;Parent=Msa1096330-mRNA-1
chr7_3 CDS 20655608 20655820 20655608 ID=Msa1096330-mRNA-1:cds;Parent=Msa1096330-mRNA-1
chr7_3 CDS 20655231 20655431 20655231 ID=Msa1096330-mRNA-1:cds;Parent=Msa1096330-mRNA-1
chr7_3 CDS 20654039 20654169 20654039 ID=Msa1096330-mRNA-1:cds;Parent=Msa1096330-mRNA-1
chr7_3 CDS 20653607 20653676 20653607 ID=Msa1096330-mRNA-1:cds;Parent=Msa1096330-mRNA-1
chr7_3 CDS 20652851 20653171 20652851 ID=Msa1096330-mRNA-1:cds;Parent=Msa1096330-mRNA-1
chr7_3 three_prime_UTR 20652490 20652850 20652490 ID=Msa1096330-mRNA-1:three_prime_utr;Parent=Msa1096330-mRNA-1
Gene Sequence

>Msa1096330

ATGACAGCAGTAGGAAGAATGAAGTTAGGATCACAAGGAATGGAAGTGTCCTTGCAAGGACTTGGTTGCATGAGCATGTCAGGTGTCTATGGTCCTCCTAAGCCTGAACCTGACATGATTGCTCTCATTCACCATGCCATACAATCTGGTGTCACTTTTCTTGATACCTCTGACATCTATGGCCCTCACACCAACGAACTCCTTCTTGGAAAGGCTTTAAGAGGAGGAGTGAGAGAGAAGGTTGAATTGGCTACAAAGTTTGGAGCCAAATATATTGAAGGGAAATTTGAGATCTTTGGTGATCCAGCTTACGTGAGAGAAGCTTGTGAAGCTAGCTTGAAGAGACTTGATATTGATTGCATTGATCTCTATTATCAACATCGTATTGATACTCGTCTTCCAATTGAAATCACGTACATTGGTTTGTCTGAAGCCTCAGCTTCAACAATCAGAAGAGCACATGCAGTTCATCCAATAACAGCTGTGCAATTGGAGTGGTCACTATGGTCAAGAGATGTTGAGGAAGAAATAATTCCAACTTGCAGGGAACTTGGTATTGGAATTGTTGCATATAGTCCTCTTGGACGAGGATTCTTTTCATCAGGAACAAAGATTTATATGCCTAGATTTCAACCTGAAAACCTGCAGCAGAATCAGACTATATTTGAGAGGGTTAATGAACTGGCTACAAAGAAGGGATGTACTCCGTCTCAGCTTGCATTGGCATGGCTTCATCACCAAGGAAACGACGTGTGCCCGATACCTGGAACCACCAAAATGGAGAACTTTAATCAAAACATTGGTGCTTTATCTGTTAAACTAACACAAGAAGAAATGGCAGAACTCGAGTCTTTAGCAGACGCTGTCAAGGGTGGTAGATATGGGGATGAAATAAGTACATGGAAGAATTCTGATACTCCACCACTCTCTTCATGA
Protein sequence

>Msa1096330

MTAVGRMKLGSQGMEVSLQGLGCMSMSGVYGPPKPEPDMIALIHHAIQSGVTFLDTSDIYGPHTNELLLGKALRGGVREKVELATKFGAKYIEGKFEIFGDPAYVREACEASLKRLDIDCIDLYYQHRIDTRLPIEITYIGLSEASASTIRRAHAVHPITAVQLEWSLWSRDVEEEIIPTCRELGIGIVAYSPLGRGFFSSGTKIYMPRFQPENLQQNQTIFERVNELATKKGCTPSQLALAWLHHQGNDVCPIPGTTKMENFNQNIGALSVKLTQEEMAELESLADAVKGGRYGDEISTWKNSDTPPLSS*