Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1112690 | CAA56254.1 | 97.345 | 113 | 3 | 0 | 1 | 113 | 1 | 113 | 1.62e-74 | 226 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1112690 | sp|Q41066|IBB2_PEA | 65.487 | 113 | 30 | 4 | 1 | 113 | 1 | 104 | 9.55e-42 | 136 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1112690 | Q40330 | 97.345 | 113 | 3 | 0 | 1 | 113 | 1 | 113 | 7.76e-75 | 226 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0781750 | Msa1112690 | 0.830666 | 2.582780e-55 | -8.615850e-47 |
| Msa1112660 | Msa1112690 | 0.806424 | 8.281014e-50 | -8.615850e-47 |
| Msa1112690 | Msa1155560 | 0.872225 | 3.746398e-67 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1112690 | MtrunA17_Chr7g0247571 | 83.186 | 113 | 18 | 1 | 1 | 113 | 39 | 150 | 1.14e-64 | 192 |
| Msa1112690 | MtrunA17_Chr7g0247471 | 80.531 | 113 | 19 | 1 | 1 | 113 | 1 | 110 | 1.15e-53 | 163 |
| Msa1112690 | MtrunA17_Chr7g0247461 | 68.067 | 119 | 29 | 5 | 1 | 113 | 17 | 132 | 3.65e-46 | 145 |
| Msa1112690 | MtrunA17_Chr7g0247481 | 70.000 | 100 | 28 | 2 | 11 | 109 | 1 | 99 | 2.51e-43 | 137 |
| Msa1112690 | MtrunA17_Chr7g0247511 | 61.739 | 115 | 40 | 3 | 1 | 113 | 1 | 113 | 3.97e-41 | 132 |
| Msa1112690 | MtrunA17_Chr7g0247531 | 60.577 | 104 | 38 | 2 | 12 | 113 | 1 | 103 | 8.30e-37 | 120 |
| Msa1112690 | MtrunA17_Chr7g0247521 | 52.727 | 110 | 43 | 3 | 5 | 113 | 1 | 102 | 9.42e-30 | 103 |
| Msa1112690 | MtrunA17_Chr7g0247451 | 56.731 | 104 | 39 | 1 | 10 | 113 | 3 | 100 | 1.71e-28 | 99.8 |
| Msa1112690 | MtrunA17_Chr7g0247541 | 46.729 | 107 | 46 | 5 | 4 | 108 | 3 | 100 | 2.35e-20 | 79.0 |
| Msa1112690 | MtrunA17_Chr7g0247581 | 46.218 | 119 | 53 | 6 | 2 | 113 | 18 | 132 | 5.30e-18 | 73.9 |
| Msa1112690 | MtrunA17_Chr7g0247491 | 43.011 | 93 | 40 | 2 | 12 | 103 | 1 | 81 | 8.89e-12 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACATAGTTAATTACATTT+TGG | 0.209853 | 7_3:+58641240 | None:intergenic |
| CAGTGTCGTTGTTCCGATAT+TGG | 0.300447 | 7_3:-58641354 | Msa1112690:CDS |
| GATGATGAAGTTAGCTTTGC+TGG | 0.342946 | 7_3:-58641538 | Msa1112690:CDS |
| GTGACATGTTTCTCCAATAT+CGG | 0.417647 | 7_3:+58641341 | None:intergenic |
| CTGTTGAAGTGAAGCCCAAA+AGG | 0.468122 | 7_3:+58641511 | None:intergenic |
| TTTGGGCTTCACTTCAACAG+TGG | 0.510225 | 7_3:-58641508 | Msa1112690:CDS |
| ATTACAGCATGCTGTTGTTG+TGG | 0.545734 | 7_3:+58641407 | None:intergenic |
| ATCACTCAACTGCTCTTCAA+CGG | 0.556499 | 7_3:-58641456 | Msa1112690:CDS |
| GCAAAAGTTGCATTTGCACA+AGG | 0.566983 | 7_3:-58641311 | Msa1112690:CDS |
| GAAGAGCAGTTGAGTGATGA+AGG | 0.573032 | 7_3:+58641461 | None:intergenic |
| GGAGTTGATGATGAACAAGA+AGG | 0.605203 | 7_3:-58641565 | Msa1112690:CDS |
| TCAGTACAACGGCACTGTGG+AGG | 0.624338 | 7_3:+58641282 | None:intergenic |
| TCGGAACAACGACACTGAGG+AGG | 0.626620 | 7_3:+58641360 | None:intergenic |
| CGGAACAACGACACTGAGGA+GGG | 0.643147 | 7_3:+58641361 | None:intergenic |
| AGTTTGTGATATCAGTACAA+CGG | 0.716192 | 7_3:+58641271 | None:intergenic |
| ATATCGGAACAACGACACTG+AGG | 0.778363 | 7_3:+58641357 | None:intergenic |
| ATATCAGTACAACGGCACTG+TGG | 0.782672 | 7_3:+58641279 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTTTGTGATATCAGTACAA+CGG | + | chr7_3:58641547-58641566 | None:intergenic | 30.0% | |
| GTGACATGTTTCTCCAATAT+CGG | + | chr7_3:58641477-58641496 | None:intergenic | 35.0% | |
| !! | GGAGTTGATGATGAACAAGA+AGG | - | chr7_3:58641250-58641269 | Msa1112690:CDS | 40.0% |
| !! | GATGATGAAGTTAGCTTTGC+TGG | - | chr7_3:58641277-58641296 | Msa1112690:CDS | 40.0% |
| !!! | AGCTTTGCTGGTTTTCCTTT+TGG | - | chr7_3:58641289-58641308 | Msa1112690:CDS | 40.0% |
| !!! | GCTTTGCTGGTTTTCCTTTT+GGG | - | chr7_3:58641290-58641309 | Msa1112690:CDS | 40.0% |
| ATCACTCAACTGCTCTTCAA+CGG | - | chr7_3:58641359-58641378 | Msa1112690:CDS | 40.0% | |
| ATTACAGCATGCTGTTGTTG+TGG | + | chr7_3:58641411-58641430 | None:intergenic | 40.0% | |
| !! | GAGGGATTGATTTTGTGCAA+GGG | + | chr7_3:58641439-58641458 | None:intergenic | 40.0% |
| GCAAAAGTTGCATTTGCACA+AGG | - | chr7_3:58641504-58641523 | Msa1112690:CDS | 40.0% | |
| CTGTTGAAGTGAAGCCCAAA+AGG | + | chr7_3:58641307-58641326 | None:intergenic | 45.0% | |
| TTTGGGCTTCACTTCAACAG+TGG | - | chr7_3:58641307-58641326 | Msa1112690:CDS | 45.0% | |
| GAAGAGCAGTTGAGTGATGA+AGG | + | chr7_3:58641357-58641376 | None:intergenic | 45.0% | |
| !! | GGAGGGATTGATTTTGTGCA+AGG | + | chr7_3:58641440-58641459 | None:intergenic | 45.0% |
| ATATCGGAACAACGACACTG+AGG | + | chr7_3:58641461-58641480 | None:intergenic | 45.0% | |
| ! | CAGTGTCGTTGTTCCGATAT+TGG | - | chr7_3:58641461-58641480 | Msa1112690:CDS | 45.0% |
| ATATCAGTACAACGGCACTG+TGG | + | chr7_3:58641539-58641558 | None:intergenic | 45.0% | |
| CGGAACAACGACACTGAGGA+GGG | + | chr7_3:58641457-58641476 | None:intergenic | 55.0% | |
| TCGGAACAACGACACTGAGG+AGG | + | chr7_3:58641458-58641477 | None:intergenic | 55.0% | |
| TCAGTACAACGGCACTGTGG+AGG | + | chr7_3:58641536-58641555 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 58641248 | 58641589 | 58641248 | ID=Msa1112690;Name=Msa1112690 |
| chr7_3 | mRNA | 58641248 | 58641589 | 58641248 | ID=Msa1112690-mRNA-1;Parent=Msa1112690;Name=Msa1112690-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|113 |
| chr7_3 | exon | 58641248 | 58641589 | 58641248 | ID=Msa1112690-mRNA-1:exon:12918;Parent=Msa1112690-mRNA-1 |
| chr7_3 | CDS | 58641248 | 58641589 | 58641248 | ID=Msa1112690-mRNA-1:cds;Parent=Msa1112690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
