Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1155560 | CAA56253.1 | 96.460 | 113 | 3 | 1 | 1 | 112 | 1 | 113 | 5.91e-72 | 219 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1155560 | sp|Q41066|IBB2_PEA | 63.393 | 112 | 33 | 3 | 1 | 112 | 1 | 104 | 7.71e-42 | 136 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1155560 | Q40329 | 96.460 | 113 | 3 | 1 | 1 | 112 | 1 | 113 | 2.82e-72 | 219 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0754680 | Msa1155560 | 0.804465 | 2.132793e-49 | -8.615850e-47 |
| Msa0797350 | Msa1155560 | 0.812806 | 3.521782e-51 | -8.615850e-47 |
| Msa0256510 | Msa1155560 | 0.812565 | 3.976480e-51 | -8.615850e-47 |
| Msa0334350 | Msa1155560 | 0.802913 | 4.476770e-49 | -8.615850e-47 |
| Msa0350620 | Msa1155560 | 0.802570 | 5.268865e-49 | -8.615850e-47 |
| Msa1112660 | Msa1155560 | 0.878824 | 2.054190e-69 | -8.615850e-47 |
| Msa1112690 | Msa1155560 | 0.872225 | 3.746398e-67 | -8.615850e-47 |
| Msa1155560 | Msa1267600 | 0.805056 | 1.604704e-49 | -8.615850e-47 |
| Msa0483650 | Msa1155560 | 0.816309 | 5.909776e-52 | -8.615850e-47 |
| Msa0948290 | Msa1155560 | 0.803928 | 2.757674e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1155560 | MtrunA17_Chr7g0247571 | 84.071 | 113 | 16 | 2 | 1 | 112 | 39 | 150 | 3.13e-63 | 189 |
| Msa1155560 | MtrunA17_Chr7g0247471 | 82.000 | 100 | 15 | 1 | 13 | 112 | 14 | 110 | 5.66e-54 | 164 |
| Msa1155560 | MtrunA17_Chr7g0247481 | 73.000 | 100 | 25 | 2 | 10 | 108 | 1 | 99 | 1.49e-44 | 140 |
| Msa1155560 | MtrunA17_Chr7g0247461 | 68.067 | 119 | 28 | 6 | 1 | 112 | 17 | 132 | 5.83e-44 | 139 |
| Msa1155560 | MtrunA17_Chr7g0247511 | 58.772 | 114 | 44 | 2 | 1 | 112 | 1 | 113 | 1.24e-40 | 130 |
| Msa1155560 | MtrunA17_Chr7g0247531 | 59.615 | 104 | 39 | 2 | 11 | 112 | 1 | 103 | 3.07e-36 | 119 |
| Msa1155560 | MtrunA17_Chr7g0247521 | 55.752 | 113 | 38 | 4 | 1 | 112 | 1 | 102 | 2.91e-31 | 107 |
| Msa1155560 | MtrunA17_Chr7g0247451 | 57.843 | 102 | 37 | 1 | 11 | 112 | 5 | 100 | 5.35e-28 | 98.6 |
| Msa1155560 | MtrunA17_Chr7g0247541 | 47.170 | 106 | 46 | 4 | 4 | 107 | 3 | 100 | 1.81e-22 | 84.3 |
| Msa1155560 | MtrunA17_Chr7g0247581 | 48.246 | 114 | 48 | 6 | 6 | 112 | 23 | 132 | 6.29e-18 | 73.6 |
| Msa1155560 | MtrunA17_Chr7g0247491 | 43.011 | 93 | 40 | 2 | 11 | 102 | 1 | 81 | 1.46e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACATAGTTAATTACATTT+TGG | 0.209853 | 7_4:-43483076 | None:intergenic |
| AATGATGAAGTTAGCTTTGT+TGG | 0.320307 | 7_4:+43482778 | Msa1155560:CDS |
| CAGTGTCGTTGTACTGATAT+TGG | 0.334872 | 7_4:+43482962 | Msa1155560:CDS |
| GTGGTTGATGCTCGTTTCGA+TGG | 0.506219 | 7_4:+43482827 | Msa1155560:CDS |
| TCTGGGCTTCACTTCAACAG+TGG | 0.510225 | 7_4:+43482808 | Msa1155560:CDS |
| ATTACAGCATGCTGTTGTTG+TGG | 0.545734 | 7_4:-43482909 | None:intergenic |
| ATCACTCAACTGCTCTTCAA+CGG | 0.556499 | 7_4:+43482860 | Msa1155560:CDS |
| CTGTTGAAGTGAAGCCCAGA+AGG | 0.562746 | 7_4:-43482805 | None:intergenic |
| GAAGAGCAGTTGAGTGATGA+AGG | 0.573032 | 7_4:-43482855 | None:intergenic |
| GGAGGGATTGATCTTGTGCA+AGG | 0.602655 | 7_4:-43482938 | None:intergenic |
| GGAGTTGATGATGAACAAGA+AGG | 0.605203 | 7_4:+43482754 | Msa1155560:CDS |
| TCAGTACAACGGCACTGTGG+AGG | 0.619150 | 7_4:-43483034 | None:intergenic |
| GAGGGATTGATCTTGTGCAA+GGG | 0.625473 | 7_4:-43482937 | None:intergenic |
| TCAGTACAACGACACTGAGG+AGG | 0.627030 | 7_4:-43482956 | None:intergenic |
| CAGTACAACGACACTGAGGA+GGG | 0.637399 | 7_4:-43482955 | None:intergenic |
| GAGGAATAGATCTTGTGCAA+AGG | 0.648501 | 7_4:-43483015 | None:intergenic |
| AGTTTGTGATATCAGTACAA+CGG | 0.716192 | 7_4:-43483045 | None:intergenic |
| ATATCAGTACAACGGCACTG+TGG | 0.782672 | 7_4:-43483037 | None:intergenic |
| ATATCAGTACAACGACACTG+AGG | 0.802196 | 7_4:-43482959 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGATGAAGTTAGCTTTGT+TGG | + | chr7_4:43482778-43482797 | Msa1155560:CDS | 30.0% |
| AGTTTGTGATATCAGTACAA+CGG | - | chr7_4:43483048-43483067 | None:intergenic | 30.0% | |
| !! | GGAGTTGATGATGAACAAGA+AGG | + | chr7_4:43482754-43482773 | Msa1155560:CDS | 40.0% |
| !!! | AGCTTTGTTGGTTTTCCTTC+TGG | + | chr7_4:43482790-43482809 | Msa1155560:CDS | 40.0% |
| !!! | GCTTTGTTGGTTTTCCTTCT+GGG | + | chr7_4:43482791-43482810 | Msa1155560:CDS | 40.0% |
| ATCACTCAACTGCTCTTCAA+CGG | + | chr7_4:43482860-43482879 | Msa1155560:CDS | 40.0% | |
| ATTACAGCATGCTGTTGTTG+TGG | - | chr7_4:43482912-43482931 | None:intergenic | 40.0% | |
| ATATCAGTACAACGACACTG+AGG | - | chr7_4:43482962-43482981 | None:intergenic | 40.0% | |
| ! | CAGTGTCGTTGTACTGATAT+TGG | + | chr7_4:43482962-43482981 | Msa1155560:CDS | 40.0% |
| GAGGAATAGATCTTGTGCAA+AGG | - | chr7_4:43483018-43483037 | None:intergenic | 40.0% | |
| GAAGAGCAGTTGAGTGATGA+AGG | - | chr7_4:43482858-43482877 | None:intergenic | 45.0% | |
| GAGGGATTGATCTTGTGCAA+GGG | - | chr7_4:43482940-43482959 | None:intergenic | 45.0% | |
| ATATCAGTACAACGGCACTG+TGG | - | chr7_4:43483040-43483059 | None:intergenic | 45.0% | |
| CTGTTGAAGTGAAGCCCAGA+AGG | - | chr7_4:43482808-43482827 | None:intergenic | 50.0% | |
| TCTGGGCTTCACTTCAACAG+TGG | + | chr7_4:43482808-43482827 | Msa1155560:CDS | 50.0% | |
| ! | GTGGTTGATGCTCGTTTCGA+TGG | + | chr7_4:43482827-43482846 | Msa1155560:CDS | 50.0% |
| GGAGGGATTGATCTTGTGCA+AGG | - | chr7_4:43482941-43482960 | None:intergenic | 50.0% | |
| CAGTACAACGACACTGAGGA+GGG | - | chr7_4:43482958-43482977 | None:intergenic | 50.0% | |
| TCAGTACAACGACACTGAGG+AGG | - | chr7_4:43482959-43482978 | None:intergenic | 50.0% | |
| TCAGTACAACGGCACTGTGG+AGG | - | chr7_4:43483037-43483056 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 43482752 | 43483090 | 43482752 | ID=Msa1155560;Name=Msa1155560 |
| chr7_4 | mRNA | 43482752 | 43483090 | 43482752 | ID=Msa1155560-mRNA-1;Parent=Msa1155560;Name=Msa1155560-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|112 |
| chr7_4 | exon | 43482752 | 43483090 | 43482752 | ID=Msa1155560-mRNA-1:exon:10996;Parent=Msa1155560-mRNA-1 |
| chr7_4 | CDS | 43482752 | 43483090 | 43482752 | ID=Msa1155560-mRNA-1:cds;Parent=Msa1155560-mRNA-1 |
| Gene Sequence |
| Protein sequence |
