AlfalfaGEDB | Msa1116620

M. sativa cultivar zhongmu-4 / Msa1116620

KEGG: -
GO: -
PFAM: PF03018.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1116620	RYR51614.1	38.462	117	54	3	7	114	759	866	2.16e-13	83.2
Msa1116620	RYR51614.1	46.632	193	84	5	2	177	533	723	4.48e-41	164
Msa1116620	RYR51614.1	48.553	311	127	6	24	317	426	720	3.32e-85	285
Msa1116620	RYR51614.1	53.261	184	67	4	34	198	209	392	3.99e-51	192
Msa1116620	RYR51614.1	53.371	356	109	6	23	323	16	369	5.65e-118	370

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1116620	sp\|A0A1V1FH01\|DIR_GLYEC	76.190	189	25	5	1	174	1	184	4.10e-93	277
Msa1116620	sp\|A0A1V1FH01\|DIR_GLYEC	86.486	148	20	0	172	319	39	186	4.57e-91	272

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1116620	A0A445CL16	38.462	117	54	3	7	114	759	866	1.03e-13	83.2
Msa1116620	A0A445CL16	46.632	193	84	5	2	177	533	723	2.14e-41	164
Msa1116620	A0A445CL16	48.553	311	127	6	24	317	426	720	1.59e-85	285
Msa1116620	A0A445CL16	53.261	184	67	4	34	198	209	392	1.91e-51	192
Msa1116620	A0A445CL16	53.371	356	109	6	23	323	16	369	2.70e-118	370

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0151220	Msa1116620	0.803481	3.415982e-49	-8.615850e-47
Msa0225840	Msa1116620	0.801943	7.094280e-49	-8.615850e-47
Msa0260530	Msa1116620	0.808761	2.642484e-50	-8.615850e-47
Msa1075500	Msa1116620	0.878099	3.694253e-69	-8.615850e-47
Msa1116620	Msa1116630	0.805770	1.137272e-49	-8.615850e-47
Msa1116620	Msa1162900	0.929737	4.519548e-93	-8.615850e-47
Msa1116620	Msa1420930	0.804378	2.222762e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1116620	MtrunA17_Chr7g0259091	84.737	190	13	3	1	174	1	190	1.10e-109	317
Msa1116620	MtrunA17_Chr7g0259091	98.582	141	2	0	172	312	45	185	8.56e-100	291
Msa1116620	MtrunA17_Chr7g0259101	86.243	189	11	2	1	174	201	389	1.30e-109	324
Msa1116620	MtrunA17_Chr7g0259101	82.143	196	18	4	1	180	1	195	5.95e-106	315
Msa1116620	MtrunA17_Chr7g0259101	92.568	148	11	0	172	319	45	192	1.18e-95	288
Msa1116620	MtrunA17_Chr7g0259101	92.568	148	11	0	172	319	244	391	1.04e-94	286
Msa1116620	MtrunA17_Chr7g0259071	68.254	189	43	3	1	174	1	187	2.68e-85	255
Msa1116620	MtrunA17_Chr7g0259071	75.000	148	37	0	172	319	42	189	3.23e-80	242
Msa1116620	MtrunA17_Chr7g0259121	56.209	153	61	3	172	319	40	191	5.90e-54	176
Msa1116620	MtrunA17_Chr7g0259121	52.326	172	60	5	23	174	20	189	1.31e-52	172

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1116620	AT5G42500.1	56.818	132	56	1	172	303	40	170	2.18e-46	155
Msa1116620	AT5G42500.1	49.697	165	64	5	25	174	22	182	1.03e-42	146
Msa1116620	AT1G65870.1	53.333	150	65	3	172	319	42	188	1.92e-44	150
Msa1116620	AT1G65870.1	50.000	150	59	3	40	174	38	186	1.06e-39	138
Msa1116620	AT1G22900.1	54.545	132	59	1	172	303	48	178	8.58e-43	146
Msa1116620	AT1G22900.1	45.087	173	76	5	17	174	22	190	2.43e-37	132
Msa1116620	AT5G42510.1	53.030	132	61	1	172	303	37	167	1.15e-42	145
Msa1116620	AT5G42510.1	48.428	159	63	5	31	174	25	179	2.91e-39	137
Msa1116620	AT5G49040.1	45.205	146	79	1	172	317	43	187	1.30e-39	138
Msa1116620	AT5G49040.1	38.298	188	90	6	16	179	6	191	1.02e-33	123

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Find 92 sgRNAs with CRISPR-Local

Find 362 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTGTGGGTAAGGCTCA+AGG	0.545821	7_3:-64337896	Msa1116620:CDS
AAACTTGTGGGTAAGGCTCA+AGG	0.547993	7_3:-64343429	Msa1116620:CDS
AAATGCGTTGCCATTCGGGA+CGG	0.561926	7_3:-64337966	Msa1116620:CDS
AACCCAGCATTGTTGGGGAT+AGG	0.607331	7_3:+64343625	None:intergenic
AAGCCTATCCCCAACAATGC+TGG	0.431336	7_3:-64343628	Msa1116620:CDS
ACAAATACCATGCTAGGTTT+TGG	0.370876	7_3:+64338013	None:intergenic
ACAAATACCATGCTAGGTTT+TGG	0.370876	7_3:+64343546	None:intergenic
ACAAGTTTCGAGTCACGTTC+AGG	0.491447	7_3:+64337911	None:intergenic
ACAATGGCCATTTCTCTAAC+AGG	0.358166	7_3:+64337749	None:intergenic
ACTAATGATGGTGATGACCA+TGG	0.702330	7_3:-64337840	Msa1116620:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTACACTTCTATATCACAAG+AGG	-	chr7_3:64337945-64337964	Msa1116620:CDS	30.0%
ATCTCCTTTGGTATAATCAA+CGG	+	chr7_3:64338148-64338167	None:intergenic	30.0%
GAATACATCATATTCCACAA+TGG	+	chr7_3:64338172-64338191	None:intergenic	30.0%
TTCGTATTCCATTATTAGCT+AGG	-	chr7_3:64338189-64338208	Msa1116620:intron	30.0%
AAACTACACCTAGCTAATAA+TGG	+	chr7_3:64338200-64338219	None:intergenic	30.0%
ACCTAAAGTGAAAGTCAAAT+AGG	-	chr7_3:64338273-64338292	Msa1116620:intron	30.0%
ATTGTCCAGTTAACAATTCA+AGG	-	chr7_3:64338409-64338428	Msa1116620:intron	30.0%
TTGTCCAGTTAACAATTCAA+GGG	-	chr7_3:64338410-64338429	Msa1116620:intron	30.0%
TTTACCCTTGAATTGTTAAC+TGG	+	chr7_3:64338417-64338436	None:intergenic	30.0%
AATTGATCATGCTGAATCTA+TGG	+	chr7_3:64338585-64338604	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	64337625	64343745	64337625	ID=Msa1116620;Name=Msa1116620
chr7_3	mRNA	64337625	64343745	64337625	ID=Msa1116620-mRNA-1;Parent=Msa1116620;Name=Msa1116620-mRNA-1;_AED=0.12;_eAED=0.13;_QI=0\|0\|0\|1\|0\|0\|4\|0\|323
chr7_3	exon	64343554	64343745	64343554	ID=Msa1116620-mRNA-1:exon:15244;Parent=Msa1116620-mRNA-1
chr7_3	exon	64343346	64343526	64343346	ID=Msa1116620-mRNA-1:exon:15243;Parent=Msa1116620-mRNA-1
chr7_3	exon	64343185	64343327	64343185	ID=Msa1116620-mRNA-1:exon:15242;Parent=Msa1116620-mRNA-1
chr7_3	exon	64337625	64338080	64337625	ID=Msa1116620-mRNA-1:exon:15241;Parent=Msa1116620-mRNA-1
chr7_3	CDS	64343554	64343745	64343554	ID=Msa1116620-mRNA-1:cds;Parent=Msa1116620-mRNA-1
chr7_3	CDS	64343346	64343526	64343346	ID=Msa1116620-mRNA-1:cds;Parent=Msa1116620-mRNA-1
chr7_3	CDS	64343185	64343327	64343185	ID=Msa1116620-mRNA-1:cds;Parent=Msa1116620-mRNA-1
chr7_3	CDS	64337625	64338080	64337625	ID=Msa1116620-mRNA-1:cds;Parent=Msa1116620-mRNA-1

Gene Sequence

>Msa1116620

ATGGCAAAATCCTCATCATGCTCCACAATTCTCATTTCTTTTAACCTTACTTTCCTCGCCGTAATATCCCTTGTCACTGCCACTAATTACTATAAAAGCCTATCCCCAACAATGCTGGGTTTTCAAGAAGAGAAATTCACTCACCTTCACTTTTACTTTCACGACATTGTGTCAGGACCAAAACCTAGCATGGTAAAAAATGCGTTGCCATTCGGGACAGTTGTGGCGATGGATGACCCTTTGACGGCTGGGCCCGAACGTGAATCAAAACTTGTGGGTAAGGCTCAAGGAATTTACACTTCTATATCACAAGAGGAGATGGGACTAATGATGGTGATGACCATGGCATTCACTGATGGAGATTTCAATGGAAGCAGAAACATGATCATGAGTGAACCTGTTAGAGAAATGGCCATTATTGGTGGGACTGAAGCTTTTCGTTTCGTACGTGGTTATGCTCAAGCTAAATTTTATTCCGTTGATTATACCAAAGGAGATGCCATTGTGGAATATGATAAATTCACTCACCTTCACTTTTACTTTCACGACATTGTGTCAGGACCAAAACCTAGCATGGTATTTGTAGCTGAGCCTAATGGCAAGGTAAAAAATGCGTTGCCATTCGGGACGGTTGTGGCGATGGATGACCCATTGACGGCTGGTCCTGAACGTGACTCGAAACTTGTGGGTAAGGCTCAAGGAATTTACACTTCTATATCACAAGAGGAGATGGGACTAATGATGGTGATGACCATGGCATTCACTGATGGAGATTTCAATGGAAGTACACTTAGCATATTGGGCAGAAACATGATCATGAGTGAACCTGTTAGAGAAATGGCCATTGTTGGCGGGACTGGGGCTTTTCGTTTTGTACGTGGGTATGCTCAAGCTAAATTTTATTCCGTTGATTACACTAAAGGAGATGCCATATTGTGGAATATGATATATTCGTATTCCATTATTAGCTAG

Protein sequence

>Msa1116620

MAKSSSCSTILISFNLTFLAVISLVTATNYYKSLSPTMLGFQEEKFTHLHFYFHDIVSGPKPSMVKNALPFGTVVAMDDPLTAGPERESKLVGKAQGIYTSISQEEMGLMMVMTMAFTDGDFNGSRNMIMSEPVREMAIIGGTEAFRFVRGYAQAKFYSVDYTKGDAIVEYDKFTHLHFYFHDIVSGPKPSMVFVAEPNGKVKNALPFGTVVAMDDPLTAGPERDSKLVGKAQGIYTSISQEEMGLMMVMTMAFTDGDFNGSTLSILGRNMIMSEPVREMAIVGGTGAFRFVRGYAQAKFYSVDYTKGDAILWNMIYSYSIIS*