AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1117320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1117320 MtrunA17_Chr7g0260011 96.183 262 10 0 10 271 1 262 0.0 520
Msa1117320 MtrunA17_Chr1g0185331 56.977 258 88 9 10 266 1 236 3.77e-82 246
Msa1117320 MtrunA17_Chr1g0205211 36.806 288 113 11 40 265 6 286 5.96e-38 135
Msa1117320 MtrunA17_Chr3g0138651 41.259 143 55 4 155 270 258 398 1.50e-21 93.2
Msa1117320 MtrunA17_Chr1g0148411 42.373 118 46 3 173 270 204 319 1.91e-19 86.7
Msa1117320 MtrunA17_Chr2g0331941 43.011 93 41 3 173 256 261 350 3.97e-17 80.5
Msa1117320 MtrunA17_Chr8g0344991 56.522 46 20 0 224 269 39 84 1.17e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1117320 AT3G22550.1 36.879 282 126 11 11 262 1 260 2.49e-42 146
Msa1117320 AT3G63210.1 39.350 277 134 13 11 267 1 263 4.59e-42 145
Msa1117320 AT5G11460.1 26.394 269 117 6 68 260 44 307 5.20e-22 94.0
Msa1117320 AT2G25690.2 45.833 96 43 4 182 270 218 311 7.10e-17 79.3
Msa1117320 AT2G25690.1 45.833 96 43 4 182 270 218 311 7.10e-17 79.3
Msa1117320 AT1G53885.1 45.588 68 37 0 202 269 20 87 2.61e-13 65.9
Msa1117320 AT1G53903.1 45.588 68 37 0 202 269 20 87 2.61e-13 65.9

Find 67 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTAGTATCTAGCTATTT+TGG 0.059941 7_3:-65674874 Msa1117320:five_prime_UTR
AAAACTATCCCTTCTTTATT+TGG 0.094272 7_3:-65674643 Msa1117320:CDS
CCAAGTAATGACTCTTAATT+TGG 0.147550 7_3:-65673390 Msa1117320:three_prime_UTR
TTGAAATCTCTAATAAATTT+TGG 0.167799 7_3:+65674616 None:intergenic
CAAAAGATATATTTATTTAT+AGG 0.182500 7_3:-65674054 Msa1117320:intron
GATAAATCAAAGTGGTTTAT+TGG 0.185687 7_3:-65674913 Msa1117320:five_prime_UTR
CCAAAAGGCTTGACTATTAT+AGG 0.223407 7_3:-65674950 Msa1117320:five_prime_UTR
CTGTGATCAGCCATTAAATT+TGG 0.239971 7_3:+65674703 None:intergenic
ACTCTTAATTTGGTTGCTTC+TGG 0.254056 7_3:-65673380 Msa1117320:three_prime_UTR
CCTATAATAGTCAAGCCTTT+TGG 0.291226 7_3:+65674950 None:intergenic
GGCATTGGTCTTGCTCTTAT+AGG 0.291963 7_3:-65674442 Msa1117320:CDS
AACAATTCTAACTCATCTTA+TGG 0.293044 7_3:-65674124 Msa1117320:CDS
ATGTTATCCCCTTATGGTTT+TGG 0.298858 7_3:-65674526 Msa1117320:CDS
AGTGACCAAGCCAAATTTAA+TGG 0.303564 7_3:-65674713 Msa1117320:CDS
ACTAGAATGTTATCCCCTTA+TGG 0.303568 7_3:-65674532 Msa1117320:CDS
TCGAATATATGAGTGGTTCT+TGG 0.324933 7_3:+65674187 None:intergenic
ATATGAGTGGTTCTTGGATT+AGG 0.342533 7_3:+65674193 None:intergenic
CCAAATTAAGAGTCATTACT+TGG 0.351609 7_3:+65673390 None:intergenic
CTCACTCAAAGAAAGAAGTT+TGG 0.359826 7_3:+65674258 None:intergenic
CAATTGAATCATCATCCTTA+AGG 0.370108 7_3:+65674416 None:intergenic
TTCTGAGAGAGAACTATTGT+TGG 0.407584 7_3:+65674585 None:intergenic
CTGCTTCATCCTAAAACAAT+GGG 0.411662 7_3:+65673348 None:intergenic
TAGAGTGCATATTTGAAATC+AGG 0.419096 7_3:-65673656 Msa1117320:three_prime_UTR
AATATAGGAGAGGGCAAAGT+TGG 0.433798 7_3:+65674325 None:intergenic
GTGGATAACCAAAACCATAA+GGG 0.435130 7_3:+65674518 None:intergenic
TGCTCTTATAGGTAACCTTA+AGG 0.442219 7_3:-65674431 Msa1117320:CDS
ACTTCTTTCTTTGAGTGAGA+TGG 0.446621 7_3:-65674254 Msa1117320:CDS
TTTGGTGAACCAAATAAAGA+AGG 0.452111 7_3:+65674634 None:intergenic
CACAGAGAAATGGTGTTAGA+TGG 0.461833 7_3:-65673801 Msa1117320:CDS
TGTGGATAACCAAAACCATA+AGG 0.468014 7_3:+65674517 None:intergenic
TAGTGTCAAGAATTGAAGTT+GGG 0.468986 7_3:+65674551 None:intergenic
ACCTTCTCATCAAGAGGCAT+TGG 0.472949 7_3:-65674457 Msa1117320:CDS
ATCAGCCATTAAATTTGGCT+TGG 0.474809 7_3:+65674708 None:intergenic
AGTATTAATGCTTATTCTTG+AGG 0.478975 7_3:-65673752 Msa1117320:three_prime_UTR
TTTGTCTTTGAGCTCTCCAA+TGG 0.480930 7_3:-65673447 Msa1117320:three_prime_UTR
TCTCTTATCTCTATATCCAT+TGG 0.489719 7_3:+65673431 None:intergenic
CAAATTCCGTAAGTGTGGTT+CGG 0.500194 7_3:-65673590 Msa1117320:three_prime_UTR
GACTCAAATGGAGAAAATAT+AGG 0.506068 7_3:+65674310 None:intergenic
TATACTTGTGTGATCTCTCA+TGG 0.508508 7_3:-65674217 Msa1117320:CDS
AGAATGCAGGCACAGAGAAA+TGG 0.509379 7_3:-65673811 Msa1117320:CDS
ACCAATGCCTCTTGATGAGA+AGG 0.509626 7_3:+65674456 None:intergenic
CTAGTGTCAAGAATTGAAGT+TGG 0.516669 7_3:+65674550 None:intergenic
CCAAAATAGCTAGATACTAG+AGG 0.518062 7_3:+65674874 None:intergenic
TCTGCTTCATCCTAAAACAA+TGG 0.518966 7_3:+65673347 None:intergenic
GAGAGAACTATTGTTGGTTG+TGG 0.537065 7_3:+65674591 None:intergenic
AACAAAACCTTCTCATCAAG+AGG 0.557222 7_3:-65674463 Msa1117320:CDS
AAGTACATAAACATTCCAAA+AGG 0.561300 7_3:-65674965 Msa1117320:five_prime_UTR
TTCATCAAATTCCGTAAGTG+TGG 0.570593 7_3:-65673595 Msa1117320:three_prime_UTR
GGATATAGAGATAAGAGAGA+GGG 0.577792 7_3:-65673426 Msa1117320:three_prime_UTR
TTGGTGAACCAAATAAAGAA+GGG 0.578160 7_3:+65674635 None:intergenic
CAAAATAGCTAGATACTAGA+GGG 0.581790 7_3:+65674875 None:intergenic
CTTTCTGCAGTAGAGAATGC+AGG 0.590215 7_3:-65673824 Msa1117320:CDS
TGGATAACCAAAACCATAAG+GGG 0.590882 7_3:+65674519 None:intergenic
AATGGAGAAAATATAGGAGA+GGG 0.592606 7_3:+65674316 None:intergenic
TTTGATTTATCATCTATGCA+AGG 0.594681 7_3:+65674925 None:intergenic
TGGATATAGAGATAAGAGAG+AGG 0.618666 7_3:-65673427 Msa1117320:three_prime_UTR
AAATGGAGAAAATATAGGAG+AGG 0.628734 7_3:+65674315 None:intergenic
AATTCTGTTGAACAAAACAA+AGG 0.636708 7_3:-65674385 Msa1117320:CDS
GCTTCATCCTAAAACAATGG+GGG 0.637850 7_3:+65673350 None:intergenic
GTGCATGTTTAGATTAACCG+TGG 0.647662 7_3:+65673561 None:intergenic
TGCTTCATCCTAAAACAATG+GGG 0.650681 7_3:+65673349 None:intergenic
GCATAGATGATAAATCAAAG+TGG 0.653145 7_3:-65674921 Msa1117320:five_prime_UTR
CTTCATCCTAAAACAATGGG+GGG 0.654680 7_3:+65673351 None:intergenic
GAGTGAGATGGAGCTTTGTG+AGG 0.659407 7_3:-65674242 Msa1117320:CDS
GCAATTATCGAATATATGAG+TGG 0.666245 7_3:+65674180 None:intergenic
GTGTGGTTCGGTTAAAACCA+CGG 0.667731 7_3:-65673578 Msa1117320:three_prime_UTR
CTAGTCATGATATCATAACG+AGG 0.716962 7_3:+65673676 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAGATATATTTATTTAT+AGG - chr7_3:65674228-65674247 Msa1117320:CDS 10.0%
!! CAAAAGATATATTTATTTAT+AGG - chr7_3:65674228-65674247 Msa1117320:CDS 10.0%
!!! TTGAAATCTCTAATAAATTT+TGG + chr7_3:65673669-65673688 None:intergenic 15.0%
!!! TTTATTTCTATTTTTCCTTT+TGG - chr7_3:65674798-65674817 Msa1117320:intron 15.0%
!!! TTGAAATCTCTAATAAATTT+TGG + chr7_3:65673669-65673688 None:intergenic 15.0%
!!! TTTATTTCTATTTTTCCTTT+TGG - chr7_3:65674798-65674817 Msa1117320:intron 15.0%
!!! TTTAGCACAATTTTGATTAT+TGG + chr7_3:65673619-65673638 None:intergenic 20.0%
!!! ATTTTGTTGTCATAAAAATG+TGG + chr7_3:65673786-65673805 None:intergenic 20.0%
!!! TTTAGCACAATTTTGATTAT+TGG + chr7_3:65673619-65673638 None:intergenic 20.0%
!!! ATTTTGTTGTCATAAAAATG+TGG + chr7_3:65673786-65673805 None:intergenic 20.0%
!! TTTGATTTATCATCTATGCA+AGG + chr7_3:65673360-65673379 None:intergenic 25.0%
! GATAAATCAAAGTGGTTTAT+TGG - chr7_3:65673369-65673388 Msa1117320:three_prime_UTR 25.0%
!! TGCAGATGATTTTTCTTTTT+GGG - chr7_3:65673511-65673530 Msa1117320:three_prime_UTR 25.0%
! AAAACTATCCCTTCTTTATT+TGG - chr7_3:65673639-65673658 Msa1117320:three_prime_UTR 25.0%
! AATTCTGTTGAACAAAACAA+AGG - chr7_3:65673897-65673916 Msa1117320:intron 25.0%
!!! AACAAAGGAAATGTTTTGTT+TGG - chr7_3:65673912-65673931 Msa1117320:intron 25.0%
!!! ATTTTGTGTATCTTTGGTTT+TGG + chr7_3:65674003-65674022 None:intergenic 25.0%
!!! TTTGGAATTTTGTGTATCTT+TGG + chr7_3:65674009-65674028 None:intergenic 25.0%
! AACAATTCTAACTCATCTTA+TGG - chr7_3:65674158-65674177 Msa1117320:CDS 25.0%
!! TCATATAACTAGATTTTTGC+TGG - chr7_3:65674381-65674400 Msa1117320:CDS 25.0%
! AGTATTAATGCTTATTCTTG+AGG - chr7_3:65674530-65674549 Msa1117320:CDS 25.0%
!!! TTTCTATTTTTCCTTTTGGA+AGG - chr7_3:65674802-65674821 Msa1117320:intron 25.0%
!!! TTCTATTTTTCCTTTTGGAA+GGG - chr7_3:65674803-65674822 Msa1117320:intron 25.0%
!! TTTGATTTATCATCTATGCA+AGG + chr7_3:65673360-65673379 None:intergenic 25.0%
! GATAAATCAAAGTGGTTTAT+TGG - chr7_3:65673369-65673388 Msa1117320:three_prime_UTR 25.0%
!! TGCAGATGATTTTTCTTTTT+GGG - chr7_3:65673511-65673530 Msa1117320:three_prime_UTR 25.0%
! AAAACTATCCCTTCTTTATT+TGG - chr7_3:65673639-65673658 Msa1117320:three_prime_UTR 25.0%
! AATTCTGTTGAACAAAACAA+AGG - chr7_3:65673897-65673916 Msa1117320:intron 25.0%
!!! AACAAAGGAAATGTTTTGTT+TGG - chr7_3:65673912-65673931 Msa1117320:intron 25.0%
!!! ATTTTGTGTATCTTTGGTTT+TGG + chr7_3:65674003-65674022 None:intergenic 25.0%
!!! TTTGGAATTTTGTGTATCTT+TGG + chr7_3:65674009-65674028 None:intergenic 25.0%
! AACAATTCTAACTCATCTTA+TGG - chr7_3:65674158-65674177 Msa1117320:CDS 25.0%
!! TCATATAACTAGATTTTTGC+TGG - chr7_3:65674381-65674400 Msa1117320:CDS 25.0%
! AGTATTAATGCTTATTCTTG+AGG - chr7_3:65674530-65674549 Msa1117320:CDS 25.0%
!!! TTTCTATTTTTCCTTTTGGA+AGG - chr7_3:65674802-65674821 Msa1117320:intron 25.0%
!!! TTCTATTTTTCCTTTTGGAA+GGG - chr7_3:65674803-65674822 Msa1117320:intron 25.0%
GCATAGATGATAAATCAAAG+TGG - chr7_3:65673361-65673380 Msa1117320:three_prime_UTR 30.0%
CAAAATAGCTAGATACTAGA+GGG + chr7_3:65673410-65673429 None:intergenic 30.0%
!! CTCTAGTATCTAGCTATTTT+GGG - chr7_3:65673409-65673428 Msa1117320:three_prime_UTR 30.0%
! GTGCAGATGATTTTTCTTTT+TGG - chr7_3:65673510-65673529 Msa1117320:three_prime_UTR 30.0%
TTGGTGAACCAAATAAAGAA+GGG + chr7_3:65673650-65673669 None:intergenic 30.0%
TTTGGTGAACCAAATAAAGA+AGG + chr7_3:65673651-65673670 None:intergenic 30.0%
TAGTGTCAAGAATTGAAGTT+GGG + chr7_3:65673734-65673753 None:intergenic 30.0%
CAATTGAATCATCATCCTTA+AGG + chr7_3:65673869-65673888 None:intergenic 30.0%
AATGGAGAAAATATAGGAGA+GGG + chr7_3:65673969-65673988 None:intergenic 30.0%
AAATGGAGAAAATATAGGAG+AGG + chr7_3:65673970-65673989 None:intergenic 30.0%
GACTCAAATGGAGAAAATAT+AGG + chr7_3:65673975-65673994 None:intergenic 30.0%
GCAATTATCGAATATATGAG+TGG + chr7_3:65674105-65674124 None:intergenic 30.0%
!! GATGAGTTAGAATTGTTTTG+TGG + chr7_3:65674155-65674174 None:intergenic 30.0%
! TAGAGTGCATATTTGAAATC+AGG - chr7_3:65674626-65674645 Msa1117320:CDS 30.0%
AACAAAAAAACCCTTCCAAA+AGG + chr7_3:65674816-65674835 None:intergenic 30.0%
TCTCTTATCTCTATATCCAT+TGG + chr7_3:65674854-65674873 None:intergenic 30.0%
CCAAATTAAGAGTCATTACT+TGG + chr7_3:65674895-65674914 None:intergenic 30.0%
CCAAGTAATGACTCTTAATT+TGG - chr7_3:65674892-65674911 Msa1117320:five_prime_UTR 30.0%
GCATAGATGATAAATCAAAG+TGG - chr7_3:65673361-65673380 Msa1117320:three_prime_UTR 30.0%
CAAAATAGCTAGATACTAGA+GGG + chr7_3:65673410-65673429 None:intergenic 30.0%
!! CTCTAGTATCTAGCTATTTT+GGG - chr7_3:65673409-65673428 Msa1117320:three_prime_UTR 30.0%
! GTGCAGATGATTTTTCTTTT+TGG - chr7_3:65673510-65673529 Msa1117320:three_prime_UTR 30.0%
TTGGTGAACCAAATAAAGAA+GGG + chr7_3:65673650-65673669 None:intergenic 30.0%
TTTGGTGAACCAAATAAAGA+AGG + chr7_3:65673651-65673670 None:intergenic 30.0%
TAGTGTCAAGAATTGAAGTT+GGG + chr7_3:65673734-65673753 None:intergenic 30.0%
CAATTGAATCATCATCCTTA+AGG + chr7_3:65673869-65673888 None:intergenic 30.0%
AATGGAGAAAATATAGGAGA+GGG + chr7_3:65673969-65673988 None:intergenic 30.0%
AAATGGAGAAAATATAGGAG+AGG + chr7_3:65673970-65673989 None:intergenic 30.0%
GACTCAAATGGAGAAAATAT+AGG + chr7_3:65673975-65673994 None:intergenic 30.0%
GCAATTATCGAATATATGAG+TGG + chr7_3:65674105-65674124 None:intergenic 30.0%
!! GATGAGTTAGAATTGTTTTG+TGG + chr7_3:65674155-65674174 None:intergenic 30.0%
! TAGAGTGCATATTTGAAATC+AGG - chr7_3:65674626-65674645 Msa1117320:CDS 30.0%
AACAAAAAAACCCTTCCAAA+AGG + chr7_3:65674816-65674835 None:intergenic 30.0%
TCTCTTATCTCTATATCCAT+TGG + chr7_3:65674854-65674873 None:intergenic 30.0%
CCAAATTAAGAGTCATTACT+TGG + chr7_3:65674895-65674914 None:intergenic 30.0%
CCAAGTAATGACTCTTAATT+TGG - chr7_3:65674892-65674911 Msa1117320:five_prime_UTR 30.0%
! CCTATAATAGTCAAGCCTTT+TGG + chr7_3:65673335-65673354 None:intergenic 35.0%
CCAAAAGGCTTGACTATTAT+AGG - chr7_3:65673332-65673351 Msa1117320:three_prime_UTR 35.0%
CCAAAATAGCTAGATACTAG+AGG + chr7_3:65673411-65673430 None:intergenic 35.0%
! CCTCTAGTATCTAGCTATTT+TGG - chr7_3:65673408-65673427 Msa1117320:three_prime_UTR 35.0%
AGTGACCAAGCCAAATTTAA+TGG - chr7_3:65673569-65673588 Msa1117320:three_prime_UTR 35.0%
ATCAGCCATTAAATTTGGCT+TGG + chr7_3:65673577-65673596 None:intergenic 35.0%
CTGTGATCAGCCATTAAATT+TGG + chr7_3:65673582-65673601 None:intergenic 35.0%
! TTCTGAGAGAGAACTATTGT+TGG + chr7_3:65673700-65673719 None:intergenic 35.0%
CTAGTGTCAAGAATTGAAGT+TGG + chr7_3:65673735-65673754 None:intergenic 35.0%
ACTAGAATGTTATCCCCTTA+TGG - chr7_3:65673750-65673769 Msa1117320:three_prime_UTR 35.0%
! ATGTTATCCCCTTATGGTTT+TGG - chr7_3:65673756-65673775 Msa1117320:three_prime_UTR 35.0%
TGGATAACCAAAACCATAAG+GGG + chr7_3:65673766-65673785 None:intergenic 35.0%
GTGGATAACCAAAACCATAA+GGG + chr7_3:65673767-65673786 None:intergenic 35.0%
TGTGGATAACCAAAACCATA+AGG + chr7_3:65673768-65673787 None:intergenic 35.0%
!!! AGGTTTTGTTTGGAACAGTT+TGG + chr7_3:65673809-65673828 None:intergenic 35.0%
!!! CTTGATGAGAAGGTTTTGTT+TGG + chr7_3:65673819-65673838 None:intergenic 35.0%
AACAAAACCTTCTCATCAAG+AGG - chr7_3:65673819-65673838 Msa1117320:CDS 35.0%
TGCTCTTATAGGTAACCTTA+AGG - chr7_3:65673851-65673870 Msa1117320:intron 35.0%
!!! GTTTTGGTTTGAGACTCAAA+TGG + chr7_3:65673987-65674006 None:intergenic 35.0%
CTCACTCAAAGAAAGAAGTT+TGG + chr7_3:65674027-65674046 None:intergenic 35.0%
ACTTCTTTCTTTGAGTGAGA+TGG - chr7_3:65674028-65674047 Msa1117320:intron 35.0%
TATACTTGTGTGATCTCTCA+TGG - chr7_3:65674065-65674084 Msa1117320:CDS 35.0%
ATATGAGTGGTTCTTGGATT+AGG + chr7_3:65674092-65674111 None:intergenic 35.0%
TCGAATATATGAGTGGTTCT+TGG + chr7_3:65674098-65674117 None:intergenic 35.0%
! AGTTTGATTGTGTTTTGCAG+AGG - chr7_3:65674427-65674446 Msa1117320:CDS 35.0%
CTAGTCATGATATCATAACG+AGG + chr7_3:65674609-65674628 None:intergenic 35.0%
TTCATCAAATTCCGTAAGTG+TGG - chr7_3:65674687-65674706 Msa1117320:CDS 35.0%
! TTTTAACCGAACCACACTTA+CGG + chr7_3:65674701-65674720 None:intergenic 35.0%
TGGATATAGAGATAAGAGAG+AGG - chr7_3:65674855-65674874 Msa1117320:intron 35.0%
GGATATAGAGATAAGAGAGA+GGG - chr7_3:65674856-65674875 Msa1117320:intron 35.0%
! ACTCTTAATTTGGTTGCTTC+TGG - chr7_3:65674902-65674921 Msa1117320:five_prime_UTR 35.0%
TGCTTCATCCTAAAACAATG+GGG + chr7_3:65674936-65674955 None:intergenic 35.0%
CTGCTTCATCCTAAAACAAT+GGG + chr7_3:65674937-65674956 None:intergenic 35.0%
TCTGCTTCATCCTAAAACAA+TGG + chr7_3:65674938-65674957 None:intergenic 35.0%
! CCTATAATAGTCAAGCCTTT+TGG + chr7_3:65673335-65673354 None:intergenic 35.0%
CCAAAAGGCTTGACTATTAT+AGG - chr7_3:65673332-65673351 Msa1117320:three_prime_UTR 35.0%
CCAAAATAGCTAGATACTAG+AGG + chr7_3:65673411-65673430 None:intergenic 35.0%
! CCTCTAGTATCTAGCTATTT+TGG - chr7_3:65673408-65673427 Msa1117320:three_prime_UTR 35.0%
AGTGACCAAGCCAAATTTAA+TGG - chr7_3:65673569-65673588 Msa1117320:three_prime_UTR 35.0%
ATCAGCCATTAAATTTGGCT+TGG + chr7_3:65673577-65673596 None:intergenic 35.0%
CTGTGATCAGCCATTAAATT+TGG + chr7_3:65673582-65673601 None:intergenic 35.0%
! TTCTGAGAGAGAACTATTGT+TGG + chr7_3:65673700-65673719 None:intergenic 35.0%
CTAGTGTCAAGAATTGAAGT+TGG + chr7_3:65673735-65673754 None:intergenic 35.0%
ACTAGAATGTTATCCCCTTA+TGG - chr7_3:65673750-65673769 Msa1117320:three_prime_UTR 35.0%
! ATGTTATCCCCTTATGGTTT+TGG - chr7_3:65673756-65673775 Msa1117320:three_prime_UTR 35.0%
TGGATAACCAAAACCATAAG+GGG + chr7_3:65673766-65673785 None:intergenic 35.0%
GTGGATAACCAAAACCATAA+GGG + chr7_3:65673767-65673786 None:intergenic 35.0%
TGTGGATAACCAAAACCATA+AGG + chr7_3:65673768-65673787 None:intergenic 35.0%
!!! AGGTTTTGTTTGGAACAGTT+TGG + chr7_3:65673809-65673828 None:intergenic 35.0%
!!! CTTGATGAGAAGGTTTTGTT+TGG + chr7_3:65673819-65673838 None:intergenic 35.0%
AACAAAACCTTCTCATCAAG+AGG - chr7_3:65673819-65673838 Msa1117320:CDS 35.0%
TGCTCTTATAGGTAACCTTA+AGG - chr7_3:65673851-65673870 Msa1117320:intron 35.0%
!!! GTTTTGGTTTGAGACTCAAA+TGG + chr7_3:65673987-65674006 None:intergenic 35.0%
CTCACTCAAAGAAAGAAGTT+TGG + chr7_3:65674027-65674046 None:intergenic 35.0%
ACTTCTTTCTTTGAGTGAGA+TGG - chr7_3:65674028-65674047 Msa1117320:intron 35.0%
TATACTTGTGTGATCTCTCA+TGG - chr7_3:65674065-65674084 Msa1117320:CDS 35.0%
ATATGAGTGGTTCTTGGATT+AGG + chr7_3:65674092-65674111 None:intergenic 35.0%
TCGAATATATGAGTGGTTCT+TGG + chr7_3:65674098-65674117 None:intergenic 35.0%
! AGTTTGATTGTGTTTTGCAG+AGG - chr7_3:65674427-65674446 Msa1117320:CDS 35.0%
CTAGTCATGATATCATAACG+AGG + chr7_3:65674609-65674628 None:intergenic 35.0%
TTCATCAAATTCCGTAAGTG+TGG - chr7_3:65674687-65674706 Msa1117320:CDS 35.0%
! TTTTAACCGAACCACACTTA+CGG + chr7_3:65674701-65674720 None:intergenic 35.0%
TGGATATAGAGATAAGAGAG+AGG - chr7_3:65674855-65674874 Msa1117320:intron 35.0%
GGATATAGAGATAAGAGAGA+GGG - chr7_3:65674856-65674875 Msa1117320:intron 35.0%
! ACTCTTAATTTGGTTGCTTC+TGG - chr7_3:65674902-65674921 Msa1117320:five_prime_UTR 35.0%
TGCTTCATCCTAAAACAATG+GGG + chr7_3:65674936-65674955 None:intergenic 35.0%
CTGCTTCATCCTAAAACAAT+GGG + chr7_3:65674937-65674956 None:intergenic 35.0%
TCTGCTTCATCCTAAAACAA+TGG + chr7_3:65674938-65674957 None:intergenic 35.0%
! GAGAGAACTATTGTTGGTTG+TGG + chr7_3:65673694-65673713 None:intergenic 40.0%
AATATAGGAGAGGGCAAAGT+TGG + chr7_3:65673960-65673979 None:intergenic 40.0%
! CACAGAGAAATGGTGTTAGA+TGG - chr7_3:65674481-65674500 Msa1117320:CDS 40.0%
CAAATTCCGTAAGTGTGGTT+CGG - chr7_3:65674692-65674711 Msa1117320:CDS 40.0%
GTGCATGTTTAGATTAACCG+TGG + chr7_3:65674724-65674743 None:intergenic 40.0%
TTTGTCTTTGAGCTCTCCAA+TGG - chr7_3:65674835-65674854 Msa1117320:intron 40.0%
! TTTTTGCCCCCCATTGTTTT+AGG - chr7_3:65674925-65674944 Msa1117320:five_prime_UTR 40.0%
CTTCATCCTAAAACAATGGG+GGG + chr7_3:65674934-65674953 None:intergenic 40.0%
GCTTCATCCTAAAACAATGG+GGG + chr7_3:65674935-65674954 None:intergenic 40.0%
! GAGAGAACTATTGTTGGTTG+TGG + chr7_3:65673694-65673713 None:intergenic 40.0%
AATATAGGAGAGGGCAAAGT+TGG + chr7_3:65673960-65673979 None:intergenic 40.0%
! CACAGAGAAATGGTGTTAGA+TGG - chr7_3:65674481-65674500 Msa1117320:CDS 40.0%
CAAATTCCGTAAGTGTGGTT+CGG - chr7_3:65674692-65674711 Msa1117320:CDS 40.0%
GTGCATGTTTAGATTAACCG+TGG + chr7_3:65674724-65674743 None:intergenic 40.0%
TTTGTCTTTGAGCTCTCCAA+TGG - chr7_3:65674835-65674854 Msa1117320:intron 40.0%
! TTTTTGCCCCCCATTGTTTT+AGG - chr7_3:65674925-65674944 Msa1117320:five_prime_UTR 40.0%
CTTCATCCTAAAACAATGGG+GGG + chr7_3:65674934-65674953 None:intergenic 40.0%
GCTTCATCCTAAAACAATGG+GGG + chr7_3:65674935-65674954 None:intergenic 40.0%
! ACCTTCTCATCAAGAGGCAT+TGG - chr7_3:65673825-65673844 Msa1117320:CDS 45.0%
ACCAATGCCTCTTGATGAGA+AGG + chr7_3:65673829-65673848 None:intergenic 45.0%
!! GGCATTGGTCTTGCTCTTAT+AGG - chr7_3:65673840-65673859 Msa1117320:intron 45.0%
CTTTCTGCAGTAGAGAATGC+AGG - chr7_3:65674458-65674477 Msa1117320:CDS 45.0%
AGAATGCAGGCACAGAGAAA+TGG - chr7_3:65674471-65674490 Msa1117320:CDS 45.0%
!! GTGTGGTTCGGTTAAAACCA+CGG - chr7_3:65674704-65674723 Msa1117320:CDS 45.0%
! ACCTTCTCATCAAGAGGCAT+TGG - chr7_3:65673825-65673844 Msa1117320:CDS 45.0%
ACCAATGCCTCTTGATGAGA+AGG + chr7_3:65673829-65673848 None:intergenic 45.0%
!! GGCATTGGTCTTGCTCTTAT+AGG - chr7_3:65673840-65673859 Msa1117320:intron 45.0%
CTTTCTGCAGTAGAGAATGC+AGG - chr7_3:65674458-65674477 Msa1117320:CDS 45.0%
AGAATGCAGGCACAGAGAAA+TGG - chr7_3:65674471-65674490 Msa1117320:CDS 45.0%
!! GTGTGGTTCGGTTAAAACCA+CGG - chr7_3:65674704-65674723 Msa1117320:CDS 45.0%
! GAGTGAGATGGAGCTTTGTG+AGG - chr7_3:65674040-65674059 Msa1117320:intron 50.0%
! GAGTGAGATGGAGCTTTGTG+AGG - chr7_3:65674040-65674059 Msa1117320:intron 50.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 65673319 65674985 65673319 ID=Msa1117320;Name=Msa1117320
chr7_3 mRNA 65673319 65674985 65673319 ID=Msa1117320-mRNA-1;Parent=Msa1117320;Name=Msa1117320-mRNA-1;_AED=0.02;_eAED=0.02;_QI=112|1|1|1|0.5|0.33|3|457|271
chr7_3 exon 65673319 65673857 65673319 ID=Msa1117320-mRNA-1:exon:15684;Parent=Msa1117320-mRNA-1
chr7_3 exon 65674055 65674788 65674055 ID=Msa1117320-mRNA-1:exon:15683;Parent=Msa1117320-mRNA-1
chr7_3 exon 65674874 65674985 65674874 ID=Msa1117320-mRNA-1:exon:15682;Parent=Msa1117320-mRNA-1
chr7_3 five_prime_UTR 65674874 65674985 65674874 ID=Msa1117320-mRNA-1:five_prime_utr;Parent=Msa1117320-mRNA-1
chr7_3 CDS 65674055 65674788 65674055 ID=Msa1117320-mRNA-1:cds;Parent=Msa1117320-mRNA-1
chr7_3 CDS 65673776 65673857 65673776 ID=Msa1117320-mRNA-1:cds;Parent=Msa1117320-mRNA-1
chr7_3 three_prime_UTR 65673319 65673775 65673319 ID=Msa1117320-mRNA-1:three_prime_utr;Parent=Msa1117320-mRNA-1
Gene Sequence

>Msa1117320

ATGATTTTTCTTTTTGGGAGAGTACATATGCTTCTAAGAAATAGATCAAGAGCAGTGACCAAGCCAAATTTAATGGCTGATCACAGTTCTCAACAATCTTCCAATAATCAAAATTGTGCTAAAAAAACTATCCCTTCTTTATTTGGTTCACCAAAATTTATTAGAGATTTCAACACCACAACCAACAATAGTTCTCTCTCAGAAGCTTTGAAAAGCCCAACTTCAATTCTTGACACTAGAATGTTATCCCCTTATGGTTTTGGTTATCCACATTTTTATGACAACAAAATCCAAACTGTTCCAAACAAAACCTTCTCATCAAGAGGCATTGGTCTTGCTCTTATAGGTAACCTTAAGGATGATGATTCAATTGATGAGAATAATTCTGTTGAACAAAACAAAGGAAATGTTTTGTTTGGAACACAGCTTAGAGTTAAAGTGCCAACTTTGCCCTCTCCTATATTTTCTCCATTTGAGTCTCAAACCAAAACCAAAGATACACAAAATTCCAAACTTCTTTCTTTGAGTGAGATGGAGCTTTGTGAGGAATATACTTGTGTGATCTCTCATGGACCTAATCCAAGAACCACTCATATATTCGATAATTGCGTTGTCGAAGAGAGTTGCTGTTCTCTGCCACAAAACAATTCTAACTCATCTTATGGAAATTTTCTTAGAGTTTGTTACACTTGCAAGAAACATCTTGAGCAGACAAAAGATATATTTATTTATAGAGGAGACAAAGCTTTCTGCAGTAGAGAATGCAGGCACAGAGAAATGGTGTTAGATGGTGTAGAAAGTTTAGAAGAATTTTGA
Protein sequence

>Msa1117320

MIFLFGRVHMLLRNRSRAVTKPNLMADHSSQQSSNNQNCAKKTIPSLFGSPKFIRDFNTTTNNSSLSEALKSPTSILDTRMLSPYGFGYPHFYDNKIQTVPNKTFSSRGIGLALIGNLKDDDSIDENNSVEQNKGNVLFGTQLRVKVPTLPSPIFSPFESQTKTKDTQNSKLLSLSEMELCEEYTCVISHGPNPRTTHIFDNCVVEESCCSLPQNNSNSSYGNFLRVCYTCKKHLEQTKDIFIYRGDKAFCSRECRHREMVLDGVESLEEF*