AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1035850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1035850 MtrunA17_Chr7g0260011 96.565 262 9 0 10 271 1 262 0.0 525
Msa1035850 MtrunA17_Chr1g0185331 56.977 258 88 9 10 266 1 236 5.86e-84 251
Msa1035850 MtrunA17_Chr1g0205211 36.552 290 111 12 40 265 6 286 9.17e-37 132
Msa1035850 MtrunA17_Chr3g0138651 42.657 143 53 5 155 270 258 398 9.10e-22 94.0
Msa1035850 MtrunA17_Chr1g0148411 42.373 118 46 3 173 270 204 319 1.56e-19 86.7
Msa1035850 MtrunA17_Chr2g0331941 43.333 90 45 3 173 256 261 350 1.70e-17 81.3
Msa1035850 MtrunA17_Chr8g0344991 56.522 46 20 0 224 269 39 84 1.13e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1035850 AT3G63210.1 38.710 279 133 12 11 267 1 263 1.81e-42 146
Msa1035850 AT3G22550.1 36.879 282 126 11 11 262 1 260 4.63e-42 145
Msa1035850 AT5G11460.1 26.119 268 119 6 68 260 44 307 8.14e-21 90.9
Msa1035850 AT2G25690.2 45.833 96 43 4 182 270 218 311 5.04e-17 79.7
Msa1035850 AT2G25690.1 45.833 96 43 4 182 270 218 311 5.04e-17 79.7
Msa1035850 AT1G53885.1 44.286 70 38 1 200 269 19 87 2.22e-13 65.9
Msa1035850 AT1G53903.1 44.286 70 38 1 200 269 19 87 2.22e-13 65.9

Find 65 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTAGTATCTAGCTATTT+TGG 0.059941 7_1:-71846029 Msa1035850:five_prime_UTR
AAAACTATCCCTTCTTTATT+TGG 0.094272 7_1:-71845798 Msa1035850:CDS
CCAAGTAATGACTCTTAATT+TGG 0.147550 7_1:-71844544 Msa1035850:three_prime_UTR
TTGAAATCTCTAATAAATTT+TGG 0.167799 7_1:+71845771 None:intergenic
CAAAAGATATATTTATTTAT+AGG 0.182500 7_1:-71845209 Msa1035850:intron
GATAAATCAAAGTGGTTTAT+TGG 0.185687 7_1:-71846068 Msa1035850:five_prime_UTR
CCAAAAGGCTTGACTATTAT+AGG 0.223407 7_1:-71846105 Msa1035850:five_prime_UTR
CTGTGATCAGCCATTAAATT+TGG 0.239971 7_1:+71845858 None:intergenic
ACTCTTAATTTGGTTGCTTC+TGG 0.254056 7_1:-71844534 Msa1035850:three_prime_UTR
CCTATAATAGTCAAGCCTTT+TGG 0.291226 7_1:+71846105 None:intergenic
GGCATTGGTCTTGCTCTTAT+AGG 0.291963 7_1:-71845597 Msa1035850:CDS
AACAATTCTAACTCATCTTA+TGG 0.293044 7_1:-71845279 Msa1035850:CDS
ATGTTATCCCCTTATGGTTT+TGG 0.298858 7_1:-71845681 Msa1035850:CDS
AGTGACCAAGCCAAATTTAA+TGG 0.303564 7_1:-71845868 Msa1035850:CDS
ACTAGAATGTTATCCCCTTA+TGG 0.303568 7_1:-71845687 Msa1035850:CDS
TCGAATATATGAGTGGTTCT+TGG 0.324933 7_1:+71845342 None:intergenic
ATATGAGTGGTTCTTGGATT+AGG 0.342533 7_1:+71845348 None:intergenic
CCAAATTAAGAGTCATTACT+TGG 0.351609 7_1:+71844544 None:intergenic
CTCACTCAAAGAAAGAAGTT+TGG 0.359826 7_1:+71845413 None:intergenic
CAATTGAATCATCATCCTTA+AGG 0.370108 7_1:+71845571 None:intergenic
TTCTGAGAGAGAACTATTGT+TGG 0.407584 7_1:+71845740 None:intergenic
CTGCTTCATCCTAAAACAAT+GGG 0.411662 7_1:+71844502 None:intergenic
AATATAGGAGATGGCAAAGT+TGG 0.431921 7_1:+71845480 None:intergenic
TGCTCTTATAGGTAACCTTA+AGG 0.442219 7_1:-71845586 Msa1035850:CDS
ACTTCTTTCTTTGAGTGAGA+TGG 0.446621 7_1:-71845409 Msa1035850:CDS
TTTGGTGAACCAAATAAAGA+AGG 0.452111 7_1:+71845789 None:intergenic
GTGGATAACCAAAACCATAA+GGG 0.454650 7_1:+71845673 None:intergenic
CACAGAGAAATGGTGTTAGA+TGG 0.461833 7_1:-71844956 Msa1035850:CDS
TAGTGTCAAGAATTGAAGTT+GGG 0.468986 7_1:+71845706 None:intergenic
AGTGGATAACCAAAACCATA+AGG 0.471666 7_1:+71845672 None:intergenic
ACCTTCTCATCAAGAGGCAT+TGG 0.472949 7_1:-71845612 Msa1035850:CDS
ATCAGCCATTAAATTTGGCT+TGG 0.474809 7_1:+71845863 None:intergenic
TTTGTCTTTGAGCTCTCCAA+TGG 0.480930 7_1:-71844601 Msa1035850:three_prime_UTR
TCTCTTATCTCTATATCCAT+TGG 0.489719 7_1:+71844585 None:intergenic
CAAATTCCGTAAGTGTGGTT+CGG 0.500194 7_1:-71844745 Msa1035850:three_prime_UTR
GACTCAAATGGAGAAAATAT+AGG 0.506068 7_1:+71845465 None:intergenic
TATACTTGTGTGATCTCTCA+TGG 0.508508 7_1:-71845372 Msa1035850:CDS
AGAATGCAGGCACAGAGAAA+TGG 0.509379 7_1:-71844966 Msa1035850:CDS
ACCAATGCCTCTTGATGAGA+AGG 0.509626 7_1:+71845611 None:intergenic
AGTACTAATGCTTATTCTTG+AGG 0.509882 7_1:-71844907 Msa1035850:three_prime_UTR
CTAGTGTCAAGAATTGAAGT+TGG 0.516669 7_1:+71845705 None:intergenic
CCAAAATAGCTAGATACTAG+AGG 0.518062 7_1:+71846029 None:intergenic
TCTGCTTCATCCTAAAACAA+TGG 0.518966 7_1:+71844501 None:intergenic
GAGAGAACTATTGTTGGTTG+TGG 0.537065 7_1:+71845746 None:intergenic
AATGGAGAAAATATAGGAGA+TGG 0.552366 7_1:+71845471 None:intergenic
AACAAAACCTTCTCATCAAG+AGG 0.557222 7_1:-71845618 Msa1035850:CDS
AAGTACATAAACATTCCAAA+AGG 0.561300 7_1:-71846120 Msa1035850:five_prime_UTR
TTCATCAAATTCCGTAAGTG+TGG 0.570593 7_1:-71844750 Msa1035850:three_prime_UTR
GGATATAGAGATAAGAGAGA+GGG 0.577792 7_1:-71844580 Msa1035850:three_prime_UTR
TTGGTGAACCAAATAAAGAA+GGG 0.578160 7_1:+71845790 None:intergenic
CAAAATAGCTAGATACTAGA+GGG 0.581790 7_1:+71846030 None:intergenic
CTTTCTGCAGTAGAGAATGC+AGG 0.590215 7_1:-71844979 Msa1035850:CDS
CTAGTCATGATATCATAATG+AGG 0.592164 7_1:+71844831 None:intergenic
TGGATAACCAAAACCATAAG+GGG 0.594533 7_1:+71845674 None:intergenic
TTTGATTTATCATCTATGCA+AGG 0.594681 7_1:+71846080 None:intergenic
TGGATATAGAGATAAGAGAG+AGG 0.618666 7_1:-71844581 Msa1035850:three_prime_UTR
AATTCTGTTGAACAAAACAA+AGG 0.636708 7_1:-71845540 Msa1035850:CDS
GCTTCATCCTAAAACAATGG+GGG 0.637850 7_1:+71844504 None:intergenic
GTGCATGTTTAGATTAACCG+TGG 0.647662 7_1:+71844716 None:intergenic
TGCTTCATCCTAAAACAATG+GGG 0.650681 7_1:+71844503 None:intergenic
GCATAGATGATAAATCAAAG+TGG 0.653145 7_1:-71846076 Msa1035850:five_prime_UTR
CTTCATCCTAAAACAATGGG+GGG 0.654680 7_1:+71844505 None:intergenic
GAGTGAGATGGAGCTTTGTG+AGG 0.659407 7_1:-71845397 Msa1035850:CDS
GCAATTATCGAATATATGAG+TGG 0.666245 7_1:+71845335 None:intergenic
GTGTGGTTCGGTTAAAACCA+CGG 0.667731 7_1:-71844733 Msa1035850:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAGATATATTTATTTAT+AGG - chr7_1:71845382-71845401 Msa1035850:CDS 10.0%
!! CAAAAGATATATTTATTTAT+AGG - chr7_1:71845382-71845401 Msa1035850:CDS 10.0%
!!! TTGAAATCTCTAATAAATTT+TGG + chr7_1:71844823-71844842 None:intergenic 15.0%
!!! TTTATTTCTATTTTTCCTTT+TGG - chr7_1:71845953-71845972 Msa1035850:intron 15.0%
!!! TTGAAATCTCTAATAAATTT+TGG + chr7_1:71844823-71844842 None:intergenic 15.0%
!!! TTTATTTCTATTTTTCCTTT+TGG - chr7_1:71845953-71845972 Msa1035850:intron 15.0%
!!! TTTAGCACAATTTTGATTAT+TGG + chr7_1:71844773-71844792 None:intergenic 20.0%
!!! ATTTTGTTGTCATAAAAAAG+TGG + chr7_1:71844940-71844959 None:intergenic 20.0%
!!! TTTAGCACAATTTTGATTAT+TGG + chr7_1:71844773-71844792 None:intergenic 20.0%
!!! ATTTTGTTGTCATAAAAAAG+TGG + chr7_1:71844940-71844959 None:intergenic 20.0%
!! TTTGATTTATCATCTATGCA+AGG + chr7_1:71844514-71844533 None:intergenic 25.0%
! GATAAATCAAAGTGGTTTAT+TGG - chr7_1:71844523-71844542 Msa1035850:three_prime_UTR 25.0%
!! TGCAGATGATTTTTCTTTTT+GGG - chr7_1:71844665-71844684 Msa1035850:three_prime_UTR 25.0%
! AAAACTATCCCTTCTTTATT+TGG - chr7_1:71844793-71844812 Msa1035850:three_prime_UTR 25.0%
! AATTCTGTTGAACAAAACAA+AGG - chr7_1:71845051-71845070 Msa1035850:intron 25.0%
!!! AACAAAGGAAATGTTTTGTT+TGG - chr7_1:71845066-71845085 Msa1035850:intron 25.0%
!!! ATTTTGTGTATCTTTGGTTT+TGG + chr7_1:71845157-71845176 None:intergenic 25.0%
!!! TTTGGAATTTTGTGTATCTT+TGG + chr7_1:71845163-71845182 None:intergenic 25.0%
! AACAATTCTAACTCATCTTA+TGG - chr7_1:71845312-71845331 Msa1035850:CDS 25.0%
!! TCATATAACTAGATTTTTGC+TGG - chr7_1:71845535-71845554 Msa1035850:CDS 25.0%
!!! TTTCTATTTTTCCTTTTGGA+AGG - chr7_1:71845957-71845976 Msa1035850:intron 25.0%
!!! TTCTATTTTTCCTTTTGGAA+GGG - chr7_1:71845958-71845977 Msa1035850:intron 25.0%
!! TTTGATTTATCATCTATGCA+AGG + chr7_1:71844514-71844533 None:intergenic 25.0%
! GATAAATCAAAGTGGTTTAT+TGG - chr7_1:71844523-71844542 Msa1035850:three_prime_UTR 25.0%
!! TGCAGATGATTTTTCTTTTT+GGG - chr7_1:71844665-71844684 Msa1035850:three_prime_UTR 25.0%
! AAAACTATCCCTTCTTTATT+TGG - chr7_1:71844793-71844812 Msa1035850:three_prime_UTR 25.0%
! AATTCTGTTGAACAAAACAA+AGG - chr7_1:71845051-71845070 Msa1035850:intron 25.0%
!!! AACAAAGGAAATGTTTTGTT+TGG - chr7_1:71845066-71845085 Msa1035850:intron 25.0%
!!! ATTTTGTGTATCTTTGGTTT+TGG + chr7_1:71845157-71845176 None:intergenic 25.0%
!!! TTTGGAATTTTGTGTATCTT+TGG + chr7_1:71845163-71845182 None:intergenic 25.0%
! AACAATTCTAACTCATCTTA+TGG - chr7_1:71845312-71845331 Msa1035850:CDS 25.0%
!! TCATATAACTAGATTTTTGC+TGG - chr7_1:71845535-71845554 Msa1035850:CDS 25.0%
!!! TTTCTATTTTTCCTTTTGGA+AGG - chr7_1:71845957-71845976 Msa1035850:intron 25.0%
!!! TTCTATTTTTCCTTTTGGAA+GGG - chr7_1:71845958-71845977 Msa1035850:intron 25.0%
GCATAGATGATAAATCAAAG+TGG - chr7_1:71844515-71844534 Msa1035850:three_prime_UTR 30.0%
CAAAATAGCTAGATACTAGA+GGG + chr7_1:71844564-71844583 None:intergenic 30.0%
!! CTCTAGTATCTAGCTATTTT+GGG - chr7_1:71844563-71844582 Msa1035850:three_prime_UTR 30.0%
! GTGCAGATGATTTTTCTTTT+TGG - chr7_1:71844664-71844683 Msa1035850:three_prime_UTR 30.0%
TTGGTGAACCAAATAAAGAA+GGG + chr7_1:71844804-71844823 None:intergenic 30.0%
TTTGGTGAACCAAATAAAGA+AGG + chr7_1:71844805-71844824 None:intergenic 30.0%
TAGTGTCAAGAATTGAAGTT+GGG + chr7_1:71844888-71844907 None:intergenic 30.0%
CAATTGAATCATCATCCTTA+AGG + chr7_1:71845023-71845042 None:intergenic 30.0%
AATGGAGAAAATATAGGAGA+TGG + chr7_1:71845123-71845142 None:intergenic 30.0%
GACTCAAATGGAGAAAATAT+AGG + chr7_1:71845129-71845148 None:intergenic 30.0%
GCAATTATCGAATATATGAG+TGG + chr7_1:71845259-71845278 None:intergenic 30.0%
!! GATGAGTTAGAATTGTTTTG+TGG + chr7_1:71845309-71845328 None:intergenic 30.0%
AGTACTAATGCTTATTCTTG+AGG - chr7_1:71845684-71845703 Msa1035850:CDS 30.0%
CTAGTCATGATATCATAATG+AGG + chr7_1:71845763-71845782 None:intergenic 30.0%
AACAAAAAAACCCTTCCAAA+AGG + chr7_1:71845971-71845990 None:intergenic 30.0%
TCTCTTATCTCTATATCCAT+TGG + chr7_1:71846009-71846028 None:intergenic 30.0%
CCAAATTAAGAGTCATTACT+TGG + chr7_1:71846050-71846069 None:intergenic 30.0%
CCAAGTAATGACTCTTAATT+TGG - chr7_1:71846047-71846066 Msa1035850:five_prime_UTR 30.0%
GCATAGATGATAAATCAAAG+TGG - chr7_1:71844515-71844534 Msa1035850:three_prime_UTR 30.0%
CAAAATAGCTAGATACTAGA+GGG + chr7_1:71844564-71844583 None:intergenic 30.0%
!! CTCTAGTATCTAGCTATTTT+GGG - chr7_1:71844563-71844582 Msa1035850:three_prime_UTR 30.0%
! GTGCAGATGATTTTTCTTTT+TGG - chr7_1:71844664-71844683 Msa1035850:three_prime_UTR 30.0%
TTGGTGAACCAAATAAAGAA+GGG + chr7_1:71844804-71844823 None:intergenic 30.0%
TTTGGTGAACCAAATAAAGA+AGG + chr7_1:71844805-71844824 None:intergenic 30.0%
TAGTGTCAAGAATTGAAGTT+GGG + chr7_1:71844888-71844907 None:intergenic 30.0%
CAATTGAATCATCATCCTTA+AGG + chr7_1:71845023-71845042 None:intergenic 30.0%
AATGGAGAAAATATAGGAGA+TGG + chr7_1:71845123-71845142 None:intergenic 30.0%
GACTCAAATGGAGAAAATAT+AGG + chr7_1:71845129-71845148 None:intergenic 30.0%
GCAATTATCGAATATATGAG+TGG + chr7_1:71845259-71845278 None:intergenic 30.0%
!! GATGAGTTAGAATTGTTTTG+TGG + chr7_1:71845309-71845328 None:intergenic 30.0%
AGTACTAATGCTTATTCTTG+AGG - chr7_1:71845684-71845703 Msa1035850:CDS 30.0%
CTAGTCATGATATCATAATG+AGG + chr7_1:71845763-71845782 None:intergenic 30.0%
AACAAAAAAACCCTTCCAAA+AGG + chr7_1:71845971-71845990 None:intergenic 30.0%
TCTCTTATCTCTATATCCAT+TGG + chr7_1:71846009-71846028 None:intergenic 30.0%
CCAAATTAAGAGTCATTACT+TGG + chr7_1:71846050-71846069 None:intergenic 30.0%
CCAAGTAATGACTCTTAATT+TGG - chr7_1:71846047-71846066 Msa1035850:five_prime_UTR 30.0%
! CCTATAATAGTCAAGCCTTT+TGG + chr7_1:71844489-71844508 None:intergenic 35.0%
CCAAAAGGCTTGACTATTAT+AGG - chr7_1:71844486-71844505 Msa1035850:three_prime_UTR 35.0%
CCAAAATAGCTAGATACTAG+AGG + chr7_1:71844565-71844584 None:intergenic 35.0%
! CCTCTAGTATCTAGCTATTT+TGG - chr7_1:71844562-71844581 Msa1035850:three_prime_UTR 35.0%
AGTGACCAAGCCAAATTTAA+TGG - chr7_1:71844723-71844742 Msa1035850:three_prime_UTR 35.0%
ATCAGCCATTAAATTTGGCT+TGG + chr7_1:71844731-71844750 None:intergenic 35.0%
CTGTGATCAGCCATTAAATT+TGG + chr7_1:71844736-71844755 None:intergenic 35.0%
! TTCTGAGAGAGAACTATTGT+TGG + chr7_1:71844854-71844873 None:intergenic 35.0%
CTAGTGTCAAGAATTGAAGT+TGG + chr7_1:71844889-71844908 None:intergenic 35.0%
ACTAGAATGTTATCCCCTTA+TGG - chr7_1:71844904-71844923 Msa1035850:three_prime_UTR 35.0%
! ATGTTATCCCCTTATGGTTT+TGG - chr7_1:71844910-71844929 Msa1035850:three_prime_UTR 35.0%
TGGATAACCAAAACCATAAG+GGG + chr7_1:71844920-71844939 None:intergenic 35.0%
GTGGATAACCAAAACCATAA+GGG + chr7_1:71844921-71844940 None:intergenic 35.0%
AGTGGATAACCAAAACCATA+AGG + chr7_1:71844922-71844941 None:intergenic 35.0%
!!! AGGTTTTGTTTGGAACAGTT+TGG + chr7_1:71844963-71844982 None:intergenic 35.0%
!!! CTTGATGAGAAGGTTTTGTT+TGG + chr7_1:71844973-71844992 None:intergenic 35.0%
AACAAAACCTTCTCATCAAG+AGG - chr7_1:71844973-71844992 Msa1035850:CDS 35.0%
TGCTCTTATAGGTAACCTTA+AGG - chr7_1:71845005-71845024 Msa1035850:intron 35.0%
AATATAGGAGATGGCAAAGT+TGG + chr7_1:71845114-71845133 None:intergenic 35.0%
!!! GTTTTGGTTTGAGACTCAAA+TGG + chr7_1:71845141-71845160 None:intergenic 35.0%
CTCACTCAAAGAAAGAAGTT+TGG + chr7_1:71845181-71845200 None:intergenic 35.0%
ACTTCTTTCTTTGAGTGAGA+TGG - chr7_1:71845182-71845201 Msa1035850:intron 35.0%
TATACTTGTGTGATCTCTCA+TGG - chr7_1:71845219-71845238 Msa1035850:CDS 35.0%
ATATGAGTGGTTCTTGGATT+AGG + chr7_1:71845246-71845265 None:intergenic 35.0%
TCGAATATATGAGTGGTTCT+TGG + chr7_1:71845252-71845271 None:intergenic 35.0%
! AGTTTGATTGTGTTTTGCAG+AGG - chr7_1:71845581-71845600 Msa1035850:CDS 35.0%
TTCATCAAATTCCGTAAGTG+TGG - chr7_1:71845841-71845860 Msa1035850:CDS 35.0%
! TTTTAACCGAACCACACTTA+CGG + chr7_1:71845855-71845874 None:intergenic 35.0%
TGGATATAGAGATAAGAGAG+AGG - chr7_1:71846010-71846029 Msa1035850:intron 35.0%
GGATATAGAGATAAGAGAGA+GGG - chr7_1:71846011-71846030 Msa1035850:intron 35.0%
! ACTCTTAATTTGGTTGCTTC+TGG - chr7_1:71846057-71846076 Msa1035850:five_prime_UTR 35.0%
TGCTTCATCCTAAAACAATG+GGG + chr7_1:71846091-71846110 None:intergenic 35.0%
CTGCTTCATCCTAAAACAAT+GGG + chr7_1:71846092-71846111 None:intergenic 35.0%
TCTGCTTCATCCTAAAACAA+TGG + chr7_1:71846093-71846112 None:intergenic 35.0%
! CCTATAATAGTCAAGCCTTT+TGG + chr7_1:71844489-71844508 None:intergenic 35.0%
CCAAAAGGCTTGACTATTAT+AGG - chr7_1:71844486-71844505 Msa1035850:three_prime_UTR 35.0%
CCAAAATAGCTAGATACTAG+AGG + chr7_1:71844565-71844584 None:intergenic 35.0%
! CCTCTAGTATCTAGCTATTT+TGG - chr7_1:71844562-71844581 Msa1035850:three_prime_UTR 35.0%
AGTGACCAAGCCAAATTTAA+TGG - chr7_1:71844723-71844742 Msa1035850:three_prime_UTR 35.0%
ATCAGCCATTAAATTTGGCT+TGG + chr7_1:71844731-71844750 None:intergenic 35.0%
CTGTGATCAGCCATTAAATT+TGG + chr7_1:71844736-71844755 None:intergenic 35.0%
! TTCTGAGAGAGAACTATTGT+TGG + chr7_1:71844854-71844873 None:intergenic 35.0%
CTAGTGTCAAGAATTGAAGT+TGG + chr7_1:71844889-71844908 None:intergenic 35.0%
ACTAGAATGTTATCCCCTTA+TGG - chr7_1:71844904-71844923 Msa1035850:three_prime_UTR 35.0%
! ATGTTATCCCCTTATGGTTT+TGG - chr7_1:71844910-71844929 Msa1035850:three_prime_UTR 35.0%
TGGATAACCAAAACCATAAG+GGG + chr7_1:71844920-71844939 None:intergenic 35.0%
GTGGATAACCAAAACCATAA+GGG + chr7_1:71844921-71844940 None:intergenic 35.0%
AGTGGATAACCAAAACCATA+AGG + chr7_1:71844922-71844941 None:intergenic 35.0%
!!! AGGTTTTGTTTGGAACAGTT+TGG + chr7_1:71844963-71844982 None:intergenic 35.0%
!!! CTTGATGAGAAGGTTTTGTT+TGG + chr7_1:71844973-71844992 None:intergenic 35.0%
AACAAAACCTTCTCATCAAG+AGG - chr7_1:71844973-71844992 Msa1035850:CDS 35.0%
TGCTCTTATAGGTAACCTTA+AGG - chr7_1:71845005-71845024 Msa1035850:intron 35.0%
AATATAGGAGATGGCAAAGT+TGG + chr7_1:71845114-71845133 None:intergenic 35.0%
!!! GTTTTGGTTTGAGACTCAAA+TGG + chr7_1:71845141-71845160 None:intergenic 35.0%
CTCACTCAAAGAAAGAAGTT+TGG + chr7_1:71845181-71845200 None:intergenic 35.0%
ACTTCTTTCTTTGAGTGAGA+TGG - chr7_1:71845182-71845201 Msa1035850:intron 35.0%
TATACTTGTGTGATCTCTCA+TGG - chr7_1:71845219-71845238 Msa1035850:CDS 35.0%
ATATGAGTGGTTCTTGGATT+AGG + chr7_1:71845246-71845265 None:intergenic 35.0%
TCGAATATATGAGTGGTTCT+TGG + chr7_1:71845252-71845271 None:intergenic 35.0%
! AGTTTGATTGTGTTTTGCAG+AGG - chr7_1:71845581-71845600 Msa1035850:CDS 35.0%
TTCATCAAATTCCGTAAGTG+TGG - chr7_1:71845841-71845860 Msa1035850:CDS 35.0%
! TTTTAACCGAACCACACTTA+CGG + chr7_1:71845855-71845874 None:intergenic 35.0%
TGGATATAGAGATAAGAGAG+AGG - chr7_1:71846010-71846029 Msa1035850:intron 35.0%
GGATATAGAGATAAGAGAGA+GGG - chr7_1:71846011-71846030 Msa1035850:intron 35.0%
! ACTCTTAATTTGGTTGCTTC+TGG - chr7_1:71846057-71846076 Msa1035850:five_prime_UTR 35.0%
TGCTTCATCCTAAAACAATG+GGG + chr7_1:71846091-71846110 None:intergenic 35.0%
CTGCTTCATCCTAAAACAAT+GGG + chr7_1:71846092-71846111 None:intergenic 35.0%
TCTGCTTCATCCTAAAACAA+TGG + chr7_1:71846093-71846112 None:intergenic 35.0%
! GAGAGAACTATTGTTGGTTG+TGG + chr7_1:71844848-71844867 None:intergenic 40.0%
! CACAGAGAAATGGTGTTAGA+TGG - chr7_1:71845635-71845654 Msa1035850:CDS 40.0%
CAAATTCCGTAAGTGTGGTT+CGG - chr7_1:71845846-71845865 Msa1035850:CDS 40.0%
GTGCATGTTTAGATTAACCG+TGG + chr7_1:71845878-71845897 None:intergenic 40.0%
TTTGTCTTTGAGCTCTCCAA+TGG - chr7_1:71845990-71846009 Msa1035850:intron 40.0%
! TTTTTGCCCCCCATTGTTTT+AGG - chr7_1:71846080-71846099 Msa1035850:five_prime_UTR 40.0%
CTTCATCCTAAAACAATGGG+GGG + chr7_1:71846089-71846108 None:intergenic 40.0%
GCTTCATCCTAAAACAATGG+GGG + chr7_1:71846090-71846109 None:intergenic 40.0%
! GAGAGAACTATTGTTGGTTG+TGG + chr7_1:71844848-71844867 None:intergenic 40.0%
! CACAGAGAAATGGTGTTAGA+TGG - chr7_1:71845635-71845654 Msa1035850:CDS 40.0%
CAAATTCCGTAAGTGTGGTT+CGG - chr7_1:71845846-71845865 Msa1035850:CDS 40.0%
GTGCATGTTTAGATTAACCG+TGG + chr7_1:71845878-71845897 None:intergenic 40.0%
TTTGTCTTTGAGCTCTCCAA+TGG - chr7_1:71845990-71846009 Msa1035850:intron 40.0%
! TTTTTGCCCCCCATTGTTTT+AGG - chr7_1:71846080-71846099 Msa1035850:five_prime_UTR 40.0%
CTTCATCCTAAAACAATGGG+GGG + chr7_1:71846089-71846108 None:intergenic 40.0%
GCTTCATCCTAAAACAATGG+GGG + chr7_1:71846090-71846109 None:intergenic 40.0%
! ACCTTCTCATCAAGAGGCAT+TGG - chr7_1:71844979-71844998 Msa1035850:CDS 45.0%
ACCAATGCCTCTTGATGAGA+AGG + chr7_1:71844983-71845002 None:intergenic 45.0%
!! GGCATTGGTCTTGCTCTTAT+AGG - chr7_1:71844994-71845013 Msa1035850:intron 45.0%
CTTTCTGCAGTAGAGAATGC+AGG - chr7_1:71845612-71845631 Msa1035850:CDS 45.0%
AGAATGCAGGCACAGAGAAA+TGG - chr7_1:71845625-71845644 Msa1035850:CDS 45.0%
!! GTGTGGTTCGGTTAAAACCA+CGG - chr7_1:71845858-71845877 Msa1035850:CDS 45.0%
! ACCTTCTCATCAAGAGGCAT+TGG - chr7_1:71844979-71844998 Msa1035850:CDS 45.0%
ACCAATGCCTCTTGATGAGA+AGG + chr7_1:71844983-71845002 None:intergenic 45.0%
!! GGCATTGGTCTTGCTCTTAT+AGG - chr7_1:71844994-71845013 Msa1035850:intron 45.0%
CTTTCTGCAGTAGAGAATGC+AGG - chr7_1:71845612-71845631 Msa1035850:CDS 45.0%
AGAATGCAGGCACAGAGAAA+TGG - chr7_1:71845625-71845644 Msa1035850:CDS 45.0%
!! GTGTGGTTCGGTTAAAACCA+CGG - chr7_1:71845858-71845877 Msa1035850:CDS 45.0%
! GAGTGAGATGGAGCTTTGTG+AGG - chr7_1:71845194-71845213 Msa1035850:intron 50.0%
! GAGTGAGATGGAGCTTTGTG+AGG - chr7_1:71845194-71845213 Msa1035850:intron 50.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 71844473 71846140 71844473 ID=Msa1035850;Name=Msa1035850
chr7_1 mRNA 71844473 71846140 71844473 ID=Msa1035850-mRNA-1;Parent=Msa1035850;Name=Msa1035850-mRNA-1;_AED=0.02;_eAED=0.02;_QI=112|1|1|1|0.5|0.33|3|458|271
chr7_1 exon 71844473 71845012 71844473 ID=Msa1035850-mRNA-1:exon:17432;Parent=Msa1035850-mRNA-1
chr7_1 exon 71845210 71845943 71845210 ID=Msa1035850-mRNA-1:exon:17431;Parent=Msa1035850-mRNA-1
chr7_1 exon 71846029 71846140 71846029 ID=Msa1035850-mRNA-1:exon:17430;Parent=Msa1035850-mRNA-1
chr7_1 five_prime_UTR 71846029 71846140 71846029 ID=Msa1035850-mRNA-1:five_prime_utr;Parent=Msa1035850-mRNA-1
chr7_1 CDS 71845210 71845943 71845210 ID=Msa1035850-mRNA-1:cds;Parent=Msa1035850-mRNA-1
chr7_1 CDS 71844931 71845012 71844931 ID=Msa1035850-mRNA-1:cds;Parent=Msa1035850-mRNA-1
chr7_1 three_prime_UTR 71844473 71844930 71844473 ID=Msa1035850-mRNA-1:three_prime_utr;Parent=Msa1035850-mRNA-1
Gene Sequence

>Msa1035850

ATGATTTTTCTTTTTGGGAGAGTACATATGCTTCTAAGAAATAGATCAAGAGCAGTGACCAAGCCAAATTTAATGGCTGATCACAGTTCTCAACAATCTTCCAATAATCAAAATTGTGCTAAAAAAACTATCCCTTCTTTATTTGGTTCACCAAAATTTATTAGAGATTTCAACACCACAACCAACAATAGTTCTCTCTCAGAAGCTTTGAAAAGCCCAACTTCAATTCTTGACACTAGAATGTTATCCCCTTATGGTTTTGGTTATCCACTTTTTTATGACAACAAAATCCAAACTGTTCCAAACAAAACCTTCTCATCAAGAGGCATTGGTCTTGCTCTTATAGGTAACCTTAAGGATGATGATTCAATTGATGAGAATAATTCTGTTGAACAAAACAAAGGAAATGTTTTGTTTGGAACACAGCTTAGAGTTAAAGTACCAACTTTGCCATCTCCTATATTTTCTCCATTTGAGTCTCAAACCAAAACCAAAGATACACAAAATTCCAAACTTCTTTCTTTGAGTGAGATGGAGCTTTGTGAGGAATATACTTGTGTGATCTCTCATGGACCTAATCCAAGAACCACTCATATATTCGATAATTGCGTTGTCGAAGAGAGTTTCTGTTCTCTGCCACAAAACAATTCTAACTCATCTTATGGAAATTTTCTTAGAGTTTGTTACACTTGCAAGAAACATCTTGAGCAGACAAAAGATATATTTATTTATAGAGGAGACAAAGCTTTCTGCAGTAGAGAATGCAGGCACAGAGAAATGGTGTTAGATGGTGTAGAAAGTTTAGAAGAATTTTGA
Protein sequence

>Msa1035850

MIFLFGRVHMLLRNRSRAVTKPNLMADHSSQQSSNNQNCAKKTIPSLFGSPKFIRDFNTTTNNSSLSEALKSPTSILDTRMLSPYGFGYPLFYDNKIQTVPNKTFSSRGIGLALIGNLKDDDSIDENNSVEQNKGNVLFGTQLRVKVPTLPSPIFSPFESQTKTKDTQNSKLLSLSEMELCEEYTCVISHGPNPRTTHIFDNCVVEESFCSLPQNNSNSSYGNFLRVCYTCKKHLEQTKDIFIYRGDKAFCSRECRHREMVLDGVESLEEF*